Vacuolar protein sorting-associated protein 45

Overview
NameVacuolar protein sorting-associated protein 45
Smed IDSMED30015161
Length (bp)1793
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vacuolar protein sorting-associated protein 45 (SMED30015161) t-SNE clustered cells

Violin plots show distribution of expression levels for Vacuolar protein sorting-associated protein 45 (SMED30015161) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vacuolar protein sorting-associated protein 45 (SMED30015161) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vacuolar protein sorting-associated protein 45 (SMED30015161) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015161

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30015161h1SMcG0005128 Contig1766GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30015161h1SMcG0005128 Contig1766GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30015161h1SMcG0005128 Contig1766GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30015161h1SMcG0021637 Contig1766GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30015161h1SMcG0021637 Contig1766GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30015161h1SMcG0021637 Contig1766GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Human
Match: VPS45 (vacuolar protein sorting 45 homolog [Source:HGNC Symbol;Acc:HGNC:14579])

HSP 1 Score: 417.157 bits (1071), Expect = 6.319e-139
Identity = 244/553 (44.12%), Postives = 361/553 (65.28%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV+ + +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RLAE ++  I++E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q IK LL N ++ + DA RLV+LYAL Y+RH       LM + L+  GVSE   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+FV+GG T+EE+L ++N+N+   G  ++LGGT + N  SFL EV
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLM-MDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEV 547          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Human
Match: VPS45 (vacuolar protein sorting 45 homolog [Source:HGNC Symbol;Acc:HGNC:14579])

HSP 1 Score: 407.912 bits (1047), Expect = 2.691e-134
Identity = 239/545 (43.85%), Postives = 356/545 (65.32%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILN 1682
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV+ + +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RLAE ++  I++E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q IK LL N ++ + DA RLV+LYAL Y+RH       LM + L+  GVSE   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+FV+GG T+EE+L ++N+N+   G  ++LGGT + N
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLM-MDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHN 539          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Human
Match: VPS45 (vacuolar protein sorting 45 homolog [Source:HGNC Symbol;Acc:HGNC:14579])

HSP 1 Score: 383.645 bits (984), Expect = 2.268e-126
Identity = 227/516 (43.99%), Postives = 335/516 (64.92%), Query Frame = 3
Query:  189 VFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV+ + +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RLAE ++  I++E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q IK LL N ++ + DA RLV+LYAL Y+RH       LM + L+  GVSE   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+FV+GG T+EE+L ++N+N+   G  ++LGGT + N  SFL EV
Sbjct:    2 VYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLM-MDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEV 511          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Human
Match: VPS45 (vacuolar protein sorting 45 homolog [Source:HGNC Symbol;Acc:HGNC:14579])

HSP 1 Score: 326.25 bits (835), Expect = 6.100e-105
Identity = 206/496 (41.53%), Postives = 307/496 (61.90%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAK-IDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYIS 1535
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV+ + +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+                        L   +  P IRYQ SSE A+RLAE ++  I++E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+ Q K   + + ++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q IK LL N ++ + DA RLV+LYAL Y+RH       LM + L+  GVSE   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ 
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNI------------------------LGCCQKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLM-MDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYLG 467          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Human
Match: VPS45 (vacuolar protein sorting 45 homolog [Source:HGNC Symbol;Acc:HGNC:14579])

HSP 1 Score: 305.834 bits (782), Expect = 2.770e-97
Identity = 183/420 (43.57%), Postives = 271/420 (64.52%), Query Frame = 3
Query:  474 PFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RLAE ++  I++E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q IK LL N ++ + DA RLV+LYAL Y+RH       LM + L+  GVSE   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+FV+GG T+EE+L ++N+N+   G  ++LGGT + N  SFL EV
Sbjct:   11 PHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLM-MDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEV 424          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Celegans
Match: vps-45 (Related to yeast Vacuolar Protein Sorting factor [Source:UniProtKB/TrEMBL;Acc:H2KYN0])

HSP 1 Score: 325.094 bits (832), Expect = 6.286e-104
Identity = 209/552 (37.86%), Postives = 331/552 (59.96%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERI--TSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINR-EWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSI--KSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISG-CSPSNHKYTDLIIFVVGGVTFEESLAIHNIN----QQISGKNVILGGTQILNYHSFLLE 1703
            M+L ++ +  I +MI+   + MK+L++D ET   V+  F+ SE+M+ +VY+ +RI   +  EN+  L C+ ++RPT +N+  L KEL+ P +  Y ++F+++++K  +K LAEAD  E V+ +QE F D  P    LF LNL ++  +    +  +  R   GI +LLL LK  P +RYQ SS   +++A+ +   I RE  LFE+ +   + LLII+R  D VTPLLNQWTY+AMI+E+L + NN     +     I LS  HDEF+ +N+  NFGEI  NIK  + E Q K      ++SI+DMK F+E+YP F+K+S  VS+HV ++ E+S ++ K+ LL VSE EQ IV+     +H  C+ +++ LL N++ ++ D IRLVLLYAL+++  P  E ++L++ L  Q        Q +  L++YG +  +  +LF  + +++ I     K +KGVENIYTQH P + N+       +L+   +P +S  C     +  ++I+F+VGG T+EE+  + ++N    Q   G  V+L G+ +LN  SFL E
Sbjct:    1 MDLVQSSRKLIQDMIQLAGSQMKLLLMDGETTPTVSCAFAQSEVMQKEVYIFDRIENKTSSENIKNLKCVVFVRPTPKNIERLVKELQEPRFSQYYLYFTNTINKYDVKRLAEADKNETVREVQEVFLDGVPIRKDLFTLNLTHIFDSSFTLKEEAAERIKCGIIALLLQLKKAPAVRYQKSSPSCKKVADDVAQFIRRENGLFENSR-ADTTLLIIERSQDAVTPLLNQWTYEAMIHEMLTLTNNRCTCTD---QNIVLSELHDEFFARNITANFGEIGQNIKTLISEFQEKKHINKNLESIQDMKKFVEDYPQFKKISGTVSKHVSLVGELSNLVQKHNLLGVSEVEQAIVSD---GDHGKCINLVRGLLKNTKTREVDIIRLVLLYALRFENAPGNELNSLISQLRPQHPKIH---QTVSTLLKYGGLSRRPADLFGGE-STIDITKRFIKGLKGVENIYTQHSPYLKNMIESCQRGRLDN--YPLLSNECD----RMDNIIVFIVGGATYEEAAYVRSLNEKRMQGYGGPAVVLAGSSMLNTKSFLEE 535          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Celegans
Match: T07A9.10 (pep chromosome:WBcel235:IV:390512:393158:1 gene:WBGene00020298.1 transcript:T07A9.10.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T07A9.10)

HSP 1 Score: 103.219 bits (256), Expect = 6.368e-23
Identity = 135/583 (23.16%), Postives = 264/583 (45.28%), Query Frame = 3
Query:   84 LKEAIKSYIINMI---KNDDNGM-----KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLL-----EKELRNP--HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQN----SSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQA---KIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIV-----NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFP-----------------YISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            L+E +K  II  I   +N D+ +      VL+LD+  + VVNS  S++E+ +  V L E +T  RE +  ++ I  + P   ++++L      K   NP   Y+S  I F      +  + L+++   + +K ++E   +  P +  +F        +N ++    +++T DGI SL   L I P +R+Q+    SSE+ +R+ +KL+                 + L+++DR  D V+PLL++ T QAM+ ++    +      E G+  EI L  + D+ +    +    E+  ++   +++ +N +  +    K  S +D    +     + KM AK++ ++  ++E  +    + L ++   EQ++       H  I +  +  ++   +L    I     +RL+L++ L   +    +     N LL  + + E E QII+ ++ +      + F+ +      P +          I ++  P I N+  ++ E++L+E  F                  Y SG +    +   +IIFVVGG+T+ E    + ++++ +   VILG  +IL   SFL
Sbjct:    2 LRELVKKQIIENILRPQNYDSKLGHRKFSVLVLDKSAMVVVNSCLSLNEVFEEGVTLVEDLTRNREPMPSMDAIYIISPVAESIDILINDFSRKTKFNPGNSYRSAHIFFLDPCCDELFEKLSKSPAVKWIKTLKELNLNLKPVESQIF-------TVNSQFR-GDMTKTADGIVSLCATLNIHPTLRFQSDFAQSSEICQRVEQKLK-------EFGNEGMGTDAELVVLDRSFDLVSPLLHEVTLQAMVVDVTAFKDGVYRYTEAGDSKEIVLD-EKDQNWLDLRHKLLPEVMKSVNKMVKDFKNTNKTEPENIKNQSSKDFSTTVRTLQPYLKMKAKMAAYI-SLTEECRSKYFDSLEKIIALEQDMAVEHTPEHVRITDSQAVGRLSTFIL--PAIPTETRLRLILIFMLTIGKD---KDEQYFNRLLHHTDIPESEFQIIKRMLIWRDKTQKSQFQHRRPP---PED-------ERFIASRWDPKIKNLIEEIYERRLDEREFKVAGKKSTSDFRPAASARYGSGLAGKPREKRKIIIFVVGGITYSEMRVAYELSKK-TNTTVILGSDEILTPSSFL 551          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Celegans
Match: unc-18 (Putative acetylcholine regulator unc-18 [Source:UniProtKB/Swiss-Prot;Acc:P34815])

HSP 1 Score: 100.523 bits (249), Expect = 5.710e-22
Identity = 138/607 (22.73%), Postives = 261/607 (43.00%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMI-----KNDD-NGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKE--LRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVII------NREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISE-----INLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKE----------------TSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQ--PLISNIANQVNEQKLNEVLFPYISGC-------SPSNHKYTD----------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            M+LK+ +   ++N +     K D  +   VL++D   +++++S   M  IM+  + + E +   RE L  L  I  + PT  +++ L ++   RN  YK   + F+ + S Q    L+++     +K ++E    F P +  +F+L+  +          +    S+L R  + I ++   L  +P +RY    + + E+   + +KL  + + + ++ E      S L+IIDR  D +TPLL++ T QAM  +LL ++N+      G   E     + L  + D   E  H+++     E+  N+K F E   NK +  +K  SI+D+ M I+  P  +K   K S H+ +                 EC +     Q +D      Q +   +     +  DA++L++   L  D   R E                T   +N LL+ + +S  + + I      G     +  ++K  +   P+   K  +  E +Y   +  P+I +I     +++L+   FP+++G        +P++ +Y                  LII+++GGVTF E  A + +        V++G  +I+    FL
Sbjct:    1 MSLKQIVGHKLLNDVIRPLKKGDGRSAWNVLIVDTLAMRMLSSCCKMHNIMEEGITIVEDLNKRREPLPTLEAIYLIAPTAESIDKLIQDYCARN-LYKCAHVFFTEACSDQLFSTLSKSAAARFIKTLKEINIAFTPYESQVFNLDSPDTFFLYYNAQKQGGLTSNLERIAEQIATVCATLGEYPSLRYRADFERNVELGHLVEQKLDAYKADDPSMGEGADKARSQLIIIDRGYDAITPLLHELTLQAMCYDLLGIENDVYKYETGGSDENLEKEVLLDENDDLWVEMRHKHIAVVSQEVTKNLKKFSESKGNKGTMDSK--SIKDLSMLIKRMPQHKKELNKFSTHISL---------------AEECMKQY--QQGVDKLCKVEQDLSTGIDAEGERVRDAMKLMV--PLLIDPAVRCEDRLRLILLYILSKNGITDENLNKLLQHANISMADKETITNAAYLGLNIVTDTGRKKTWT---PT---KKERPHEQVYQSSRWVPVIKDIIEDAIDERLDTKHFPFLAGRQVNQGYRAPASARYGQWHKERGQQSNYRSGPRLIIYIIGGVTFSEMRACYEVTAARKPWEVVIGSDRIITPDKFL 579          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Celegans
Match: vps-45 (Related to yeast Vacuolar Protein Sorting factor [Source:UniProtKB/TrEMBL;Acc:H2KYN0])

HSP 1 Score: 85.5001 bits (210), Expect = 1.371e-19
Identity = 43/123 (34.96%), Postives = 79/123 (64.23%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERI--TSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNI 440
            M+L ++ +  I +MI+   + MK+L++D ET   V+  F+ SE+M+ +VY+ +RI   +  EN+  L C+ ++RPT +N+  L KEL+ P +  Y ++F+++++K  +K LAEAD  E +  +
Sbjct:    1 MDLVQSSRKLIQDMIQLAGSQMKLLLMDGETTPTVSCAFAQSEVMQKEVYIFDRIENKTSSENIKNLKCVVFVRPTPKNIERLVKELQEPRFSQYYLYFTNTINKYDVKRLAEADKNETIPKV 123          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Celegans
Match: sly-1 (SLY1 homolog [Source:UniProtKB/TrEMBL;Acc:Q20364])

HSP 1 Score: 66.2402 bits (160), Expect = 4.439e-11
Identity = 72/317 (22.71%), Postives = 145/317 (45.74%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEAD-HFELVKNIQ---ESFADFYPCDPFLFHL-----------------NLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIR--YQNSSE-VARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLL--------------NQWTYQAMINELLEVDNNCVNI---HEGEISEINLSPDH-DEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKI 968
            KVL+LD     +++ +  + ++ +  V L   I + RE L  +  + ++ P   N++L+ ++L+   Y S+  +F   L++  L+ LA A  H   V  +Q   + + +F   +  LF L                    +V +N     + L    DG+ ++   + I P IR    N++E VA++L +KLR       NL +S+ N  ++  +    L    PLL              + WTYQA+++++LE+D N V I     G+  + +++    D+ ++ +  + F  +   ++  +++ ++K  +  KI
Sbjct:   37 KVLVLDRTGQDIISPLLPVKQLRELGVTLHLLIGARREPLTDVPAVYFVSPNDENIDLMSEDLKKAMYDSFYCNFISPLARPRLESLASAAVHGGAVSQVQKVVDQYLNFISLEDDLFVLRRYSENSTFSYHAMNSPGTSDVAVN-----AMLDSIADGLFAVCATMGIVPIIRCPKGNAAEMVAKKLDQKLR------DNLRDSRNNLFTMDGVRMGLLQTSRPLLVIGDRSADLATMLHHTWTYQALMHDVLELDQNRVTISTSSTGKKKDYDMATGGSDKLWNNHKGSAFPTVAEAVQENLDDYRSKEDDIKKI 342          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Fly
Match: Vps45 (gene:FBgn0261049 transcript:FBtr0082070)

HSP 1 Score: 388.652 bits (997), Expect = 5.103e-128
Identity = 230/560 (41.07%), Postives = 349/560 (62.32%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPREN--LLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXX----XXXXXXXXPVTPLLNQWTYQAMINELLEVDN------NCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFK--EKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYI-SGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MNL   IK YI  M      GMK+++LD+ET  +++  FS S++++ +VYL ER+ S R N  L YL CI ++RPT +N+ LL  ELRNP Y +Y I+FS+ + +  +K LAE D  E V+ ++E +AD+   +P LF L + N + N  W   +L+R++ GIT++LL+LK+ P IRY+  S+ A+ LA+ +   I++E++LF+ + N         L++DRR DPVTPLL+QWTYQAM++ELL + N      NC N+ + +  E+ LS D D+FY  N+Y N+GEI S IK  MEE Q K+++  K++SI DMK FIE+YP F+KMS  V +H+ +I E+S + NK  L  VSE EQ I        H++ +Q IK L+ +  +   DA++LV LYAL+Y+RH   +TS L+ ++  + G +     I+  L+EY    ++  +LF      +++ +  NL K +KGVEN++TQH PL+      V + +  + LFP I S   P      ++++F++GG T+EE+LA+H +N   +G  VILGGT I N  SF+ EV
Sbjct:    1 MNLISGIKLYIEKMCSESGPGMKIILLDKETTSIISMAFSQSDMLQREVYLFERLDSGRSNERLKYLKCIVFIRPTKQNIQLLANELRNPKYSAYYIYFSNIIPRTDIKYLAECDESESVREVKELYADYLCVNPNLFSLGIPNCMANLNWLPDALNRSMQGITAVLLSLKLNPVIRYRAGSQAAQLLAKLIYEQITKESSLFDFRSNMDGAAPPLLLVLDRRDDPVTPLLHQWTYQAMVHELLHIKNNRLDLSNCPNVPK-DFKELVLSGDQDDFYGNNMYANYGEIGSTIKQLMEEFQRKANDHKKVESIADMKNFIESYPQFKKMSGTVQKHLCVIGELSALSNKRNLFEVSELEQEIACKA---EHSAQLQRIKKLIADERVSIDDALKLVALYALRYERHANCDTSGLLQIIKTRGGRA----AIVPSLIEYAGTHVRQGDLFNMVRITDAVKLTRNLIKGLKGVENVFTQHTPLLKETLEDVFKGRELDPLFPAINSELVPFRRPPQEVVVFIIGGATYEEALAVHQLNN--AGYKVILGGTTIHNSQSFIQEV 550          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Fly
Match: Rop (gene:FBgn0004574 transcript:FBtr0310632)

HSP 1 Score: 93.2041 bits (230), Expect = 1.662e-19
Identity = 125/577 (21.66%), Postives = 257/577 (44.54%), Query Frame = 3
Query:  135 NGM--KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPH---YKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSE----VARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGE---ISEINLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIV-----NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKE-KINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCS-------PSNHKY---------------TDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGS 1712
             GM  +VL++D+  +++V++   M EI    + L E I   RE L  ++ I  + P+  +V  L ++  NP    Y+   + F+    ++    L ++     +K ++E    F P +  +F L+  +                 + R  + I +L   L  +P +RY++  +    +A  + +KL  + + +A + E  +   S LLI+DR  D V+PLL++ T QAM  +LL + N+      G      E+ L  + D   E  H+++     ++  N+K F +  +  S++++   S+ D+   I+  P ++K  +K S H+ +  +  K        ++   EQ++        + I +H   ++ I  +L ++ +  +D +R++ LY +  +    +  + L       + +S  +  ++R L   G     N+  + +     +P    +  +  E+ Y  ++  P+I +I     E KL+   FP++ G +       P++ +Y                 LI+F+VGGV+  E    + +   +    V++G + IL+   FL ++GS
Sbjct:   35 GGMEWRVLVVDKLGMRMVSACTKMHEISAEGITLVEDINKKREPLPTMDAIYLITPSDESVRGLIRDFENPARPMYRYAHVFFTEVCPEELFNDLCKSCAAGKIKTLKEINIAFLPYECQVFSLDSPDTFQCLYSPAFASIRSKHIERIAEQIATLCATLGEYPNVRYRSDWDRNIDLAASVQQKLDAYKADDATMGEGPEKARSQLLILDRGFDCVSPLLHELTLQAMAYDLLPIVNDVYRYTPGPNQPDKEVLLDENDDLWVELRHEHIAVVSTQVTQNLKKFTDSKRMGSADKS---SMRDLSQMIKKMPQYQKELSKYSTHLHLAEDCMKSYQNYVD-KLCRVEQDLAMGTDAEGEKIKDH---MRNIVPILLDANVSNYDKVRIIALYVMIKNGISEENLTKLFT----HAQLSPKDQDMVRNLSCLGI----NVIADSRKKQYSVP----RKERTTESTYQMSRWTPVIKDIMEDCIEDKLDLRHFPFLEGRAQNTNYHAPTSARYGHWHKDKGQAQVKNVPRLIVFIVGGVSMSEMRCAYEVTNAVRNWEVLVGSSHILSPEIFLSDLGS 592          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Fly
Match: Rop (gene:FBgn0004574 transcript:FBtr0073263)

HSP 1 Score: 93.2041 bits (230), Expect = 1.662e-19
Identity = 125/577 (21.66%), Postives = 257/577 (44.54%), Query Frame = 3
Query:  135 NGM--KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPH---YKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSE----VARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGE---ISEINLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIV-----NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKE-KINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCS-------PSNHKY---------------TDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGS 1712
             GM  +VL++D+  +++V++   M EI    + L E I   RE L  ++ I  + P+  +V  L ++  NP    Y+   + F+    ++    L ++     +K ++E    F P +  +F L+  +                 + R  + I +L   L  +P +RY++  +    +A  + +KL  + + +A + E  +   S LLI+DR  D V+PLL++ T QAM  +LL + N+      G      E+ L  + D   E  H+++     ++  N+K F +  +  S++++   S+ D+   I+  P ++K  +K S H+ +  +  K        ++   EQ++        + I +H   ++ I  +L ++ +  +D +R++ LY +  +    +  + L       + +S  +  ++R L   G     N+  + +     +P    +  +  E+ Y  ++  P+I +I     E KL+   FP++ G +       P++ +Y                 LI+F+VGGV+  E    + +   +    V++G + IL+   FL ++GS
Sbjct:   35 GGMEWRVLVVDKLGMRMVSACTKMHEISAEGITLVEDINKKREPLPTMDAIYLITPSDESVRGLIRDFENPARPMYRYAHVFFTEVCPEELFNDLCKSCAAGKIKTLKEINIAFLPYECQVFSLDSPDTFQCLYSPAFASIRSKHIERIAEQIATLCATLGEYPNVRYRSDWDRNIDLAASVQQKLDAYKADDATMGEGPEKARSQLLILDRGFDCVSPLLHELTLQAMAYDLLPIVNDVYRYTPGPNQPDKEVLLDENDDLWVELRHEHIAVVSTQVTQNLKKFTDSKRMGSADKS---SMRDLSQMIKKMPQYQKELSKYSTHLHLAEDCMKSYQNYVD-KLCRVEQDLAMGTDAEGEKIKDH---MRNIVPILLDANVSNYDKVRIIALYVMIKNGISEENLTKLFT----HAQLSPKDQDMVRNLSCLGI----NVIADSRKKQYSVP----RKERTTESTYQMSRWTPVIKDIMEDCIEDKLDLRHFPFLEGRAQNTNYHAPTSARYGHWHKDKGQAQVKNVPRLIVFIVGGVSMSEMRCAYEVTNAVRNWEVLVGSSHILSPEIFLSDLGS 592          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Fly
Match: Slh (gene:FBgn0264978 transcript:FBtr0335169)

HSP 1 Score: 63.929 bits (154), Expect = 2.269e-10
Identity = 132/613 (21.53%), Postives = 255/613 (41.60%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLK-LLAEADHFELVKNIQ---ESFADFYPCDPFLFHL------NLKNVIINR----EWEISSLSRTI-DGITSLLLALKIFPQIRYQNSSE---VARRLAEKLRIHI-SREANLFESKQNXXXXXXXXXXXXXPV-----------TPLLNQWTYQAMINELLEVDNNCVNIHEGEISE-INLSPD------HDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKI------------------DSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEME----IQIIRILM------------EYGSIKSPNLFKEKINSLGIPSNLFKSIKGVEN-IYTQHQPLISNIANQVNEQKLNEVLFPYI---------SGCSPSNHK-YTDLIIFVVGGVTFEESLAIHNINQQISGKNV----ILGGTQILNYHSFLLEVGSWIGE 1724
            K+L+ D     +++ + S+ E+ +  V L  ++ S R+++  +  I +  PT  N++ ++++  +  Y  Y ++F   +++  ++ L A A H   V NI    + + +F   +   F L       L    INR    + E+ +L  +I D + +L + L   P IR   +S    VAR+L +KLR ++    ANLF    + +     +     PV           TPL + W+YQA+++++L++  N V + +   S      P       +D F+  +  + F  +   I+  +E  +N      ++                  D+   +   + + P   +    +  H  I + I   I   +L    E E+ +++ Q +D     +    +  G +E    D +RL ++Y +   + P  E   L   L  Q+   ++     +Q  + +M            E G  K+ ++F  K+ S G  S+    ++GV+N +  +H   ++ I  QV E + N     Y+             P N   + D ++F+VGG  + E   + +  +Q    NV    I G + + N   FL E+ +  GE
Sbjct:   33 KILIYDRVGQDIISPIISIKELRELGVTLHVQLHSDRDSIPDVPAIYFCLPTDENLDRIQQDFSSGLYDVYHLNFLAPITRSKIENLAAAALHAGCVANIHRVYDQYVNFISLEDDFFILKHQQSDQLSYYAINRANTRDEEMEALMDSIVDSLFALFVTLGNVPIIRCPRNSAAEMVARKLEKKLRENLWDARANLFH--MDATQAGGGVFSFQRPVLLLLDRNMDLATPLHHTWSYQALVHDVLDLGLNLVYVEDETASAGARKKPKACDLDRNDRFWMTHKGSPFPTVAEAIQEELESYRNSEEEIKRLKTSMGIEGESDIAFSLVNDTTARLTNAVNSLPQLMEKKRLIDMHTKIATAILNFIKARRLDSFFEIEEKVMSKQTLDRPLLDLLRDGE-FGQAE----DKLRLYIIYFICAQQLPESEQERLKEAL--QAAGCDLTALAYVQRWKGIMNRSPSISQATQYEGGGTKTVSMFT-KLVSQG--SSFV--MEGVKNLVVKRHNLPVTKITEQVMECRSNAETDDYLYLDPKLLKGGEVLPKNRAPFQDAVVFMVGGGNYIEYQNLVDFIKQKQTSNVQRRIIYGASTLTNARQFLKELSALGGE 631          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Fly
Match: Slh (gene:FBgn0264978 transcript:FBtr0077782)

HSP 1 Score: 63.929 bits (154), Expect = 2.269e-10
Identity = 132/613 (21.53%), Postives = 255/613 (41.60%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLK-LLAEADHFELVKNIQ---ESFADFYPCDPFLFHL------NLKNVIINR----EWEISSLSRTI-DGITSLLLALKIFPQIRYQNSSE---VARRLAEKLRIHI-SREANLFESKQNXXXXXXXXXXXXXPV-----------TPLLNQWTYQAMINELLEVDNNCVNIHEGEISE-INLSPD------HDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKI------------------DSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEME----IQIIRILM------------EYGSIKSPNLFKEKINSLGIPSNLFKSIKGVEN-IYTQHQPLISNIANQVNEQKLNEVLFPYI---------SGCSPSNHK-YTDLIIFVVGGVTFEESLAIHNINQQISGKNV----ILGGTQILNYHSFLLEVGSWIGE 1724
            K+L+ D     +++ + S+ E+ +  V L  ++ S R+++  +  I +  PT  N++ ++++  +  Y  Y ++F   +++  ++ L A A H   V NI    + + +F   +   F L       L    INR    + E+ +L  +I D + +L + L   P IR   +S    VAR+L +KLR ++    ANLF    + +     +     PV           TPL + W+YQA+++++L++  N V + +   S      P       +D F+  +  + F  +   I+  +E  +N      ++                  D+   +   + + P   +    +  H  I + I   I   +L    E E+ +++ Q +D     +    +  G +E    D +RL ++Y +   + P  E   L   L  Q+   ++     +Q  + +M            E G  K+ ++F  K+ S G  S+    ++GV+N +  +H   ++ I  QV E + N     Y+             P N   + D ++F+VGG  + E   + +  +Q    NV    I G + + N   FL E+ +  GE
Sbjct:   33 KILIYDRVGQDIISPIISIKELRELGVTLHVQLHSDRDSIPDVPAIYFCLPTDENLDRIQQDFSSGLYDVYHLNFLAPITRSKIENLAAAALHAGCVANIHRVYDQYVNFISLEDDFFILKHQQSDQLSYYAINRANTRDEEMEALMDSIVDSLFALFVTLGNVPIIRCPRNSAAEMVARKLEKKLRENLWDARANLFH--MDATQAGGGVFSFQRPVLLLLDRNMDLATPLHHTWSYQALVHDVLDLGLNLVYVEDETASAGARKKPKACDLDRNDRFWMTHKGSPFPTVAEAIQEELESYRNSEEEIKRLKTSMGIEGESDIAFSLVNDTTARLTNAVNSLPQLMEKKRLIDMHTKIATAILNFIKARRLDSFFEIEEKVMSKQTLDRPLLDLLRDGE-FGQAE----DKLRLYIIYFICAQQLPESEQERLKEAL--QAAGCDLTALAYVQRWKGIMNRSPSISQATQYEGGGTKTVSMFT-KLVSQG--SSFV--MEGVKNLVVKRHNLPVTKITEQVMECRSNAETDDYLYLDPKLLKGGEVLPKNRAPFQDAVVFMVGGGNYIEYQNLVDFIKQKQTSNVQRRIIYGASTLTNARQFLKELSALGGE 631          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Zebrafish
Match: vps45 (vacuolar protein sorting 45 homolog [Source:ZFIN;Acc:ZDB-GENE-060503-526])

HSP 1 Score: 422.55 bits (1085), Expect = 3.295e-141
Identity = 246/558 (44.09%), Postives = 371/558 (66.49%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIG 1721
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  R+N+ +L  IC+LRPT  NV  L +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + DF   +P LF LNL+ V   R WE S L R   G+TS+LL+LK  P IRYQ SS++++RLAE ++  I++E  LF+  K     LLLI+DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  NLY NFGEI +NIK  ME+  + K  +Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++ +L+ VSE EQ +      ++H++  QM++ LL +  + + DA+RLV+LYAL+Y++H      +LM   L + GVSE   +++  ++EYG   I+  +L     +++ I    FK +KGVEN+YTQHQPL+ +  +Q+ + +L +  FPY+ G S    +  D+I+F++GG T+EE+L ++N+N+ + G  ++LGGT I N  SFL EV   +G
Sbjct:    1 MNVTLAVKQYISKMIESSGPGMKVLLMDKETTSIVSVVYTQSEILQKEVYLFERIDSQNRDNMKHLKAICFLRPTKENVEHLIQELRRPKYSVYFIYFSNVISKSEVKALAEADEQEVVAEVQEFYGDFIAVNPHLFSLNLQGVSRGRSWEPSVLPRVTQGLTSVLLSLKKCPMIRYQLSSDMSKRLAESVKQIITKEYELFDFRKTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGLNNNRIDLSRVPGISKDLREVVLSAENDEFYANNLYLNFGEIGTNIKNLMEDFQKKKPKDQQKLESISDMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERQLMEVSEVEQELACQ---NDHSNAQQMLRRLLQSPRLSEIDAVRLVMLYALRYEKHSSSILPSLME-ELNRKGVSERHRKMVHAVVEYGGKRIRGSDLIT-PTDAVSITKQFFKGLKGVENVYTQHQPLLHDTLDQLIKGRLKDSQFPYL-GPSSLRDRPQDIIVFIIGGATYEEALTVYNLNRTMPGVRIVLGGTHIHNTKSFLEEVMLSVG 552          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Zebrafish
Match: stxbp1b (syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-060531-166])

HSP 1 Score: 113.235 bits (282), Expect = 8.385e-26
Identity = 128/581 (22.03%), Postives = 264/581 (45.44%), Query Frame = 3
Query:   90 EAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLE---KELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNL--LLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD----------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            E I + +I  +K      KVL++D+ ++++V+S   M++IM   + + E IT  RE L  +  I  + P+  +V  L    ++ RNP Y+   + F+ ++      LL+++   + +K + E    F P +  +F ++  +           + +   L R  + I +L   LK +P +RY    ++++ +A+ L +KL  + + +  L E      S LLI+DR  DPV+PLL++ T+QAM  +LL ++N+        + +  + E+ L  D D+ +    + +  E+ + +   +++   +K  N  +  +++++   ++  P ++K  +K S H+  ++E      +  + ++   EQ++      +       ++ I  +L ++ +   D IR++LLY         K   +  NL  LL+ + +   +  II  +   G    P + ++     G  S+  + I       ++  PL+ +I     E KL+   +PYIS  + S+   T                       +I+F+VGGVT+ E   ++ + Q       ++G T  +    +L
Sbjct:   12 EKIMNDVIRKVKKKGE-WKVLVVDKLSMRMVSSCCKMTDIMSEGITIVEDITKKREPLPTMEAIFLITPSDESVEGLINDFRDPRNPIYRGAHVFFTDAIPDSLFNLLSKSRASKSIKTLTEIHIAFLPYESQVFSVDKSDAFQDFYSPFKADVKNQVLERLAEQIATLCATLKEYPAVRYRGEYKDNAVLAQMLQDKLDGYKADDPTLGEGPDKARSQLLILDRGFDPVSPLLHELTFQAMAYDLLPIENDVYMYETSGMGDTRMKEVLLDED-DDLWMTLRHKHIAEVSTAVTKSLKDFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHL-HLAEDCMKQYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDATVTIMDKIRIILLYIF------LKNGVSEENLCKLLQHAQIPPEDSDIISNMAHMG---VPIISEQATTRKGKKSDRKERISEQTYQLSRWTPLVKDIMEDAIEDKLDPKQYPYISTRTVSSKTTTATSARYGNWHKNKSPGEIRNGPRIIVFIVGGVTYSEMRCVYEVTQANGKWEALIGSTHTITPVKYL 580          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Zebrafish
Match: stxbp1b (syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-060531-166])

HSP 1 Score: 113.235 bits (282), Expect = 8.385e-26
Identity = 128/581 (22.03%), Postives = 264/581 (45.44%), Query Frame = 3
Query:   90 EAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLE---KELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNL--LLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD----------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            E I + +I  +K      KVL++D+ ++++V+S   M++IM   + + E IT  RE L  +  I  + P+  +V  L    ++ RNP Y+   + F+ ++      LL+++   + +K + E    F P +  +F ++  +           + +   L R  + I +L   LK +P +RY    ++++ +A+ L +KL  + + +  L E      S LLI+DR  DPV+PLL++ T+QAM  +LL ++N+        + +  + E+ L  D D+ +    + +  E+ + +   +++   +K  N  +  +++++   ++  P ++K  +K S H+  ++E      +  + ++   EQ++      +       ++ I  +L ++ +   D IR++LLY         K   +  NL  LL+ + +   +  II  +   G    P + ++     G  S+  + I       ++  PL+ +I     E KL+   +PYIS  + S+   T                       +I+F+VGGVT+ E   ++ + Q       ++G T  +    +L
Sbjct:   12 EKIMNDVIRKVKKKGE-WKVLVVDKLSMRMVSSCCKMTDIMSEGITIVEDITKKREPLPTMEAIFLITPSDESVEGLINDFRDPRNPIYRGAHVFFTDAIPDSLFNLLSKSRASKSIKTLTEIHIAFLPYESQVFSVDKSDAFQDFYSPFKADVKNQVLERLAEQIATLCATLKEYPAVRYRGEYKDNAVLAQMLQDKLDGYKADDPTLGEGPDKARSQLLILDRGFDPVSPLLHELTFQAMAYDLLPIENDVYMYETSGMGDTRMKEVLLDED-DDLWMTLRHKHIAEVSTAVTKSLKDFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHL-HLAEDCMKQYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDATVTIMDKIRIILLYIF------LKNGVSEENLCKLLQHAQIPPEDSDIISNMAHMG---VPIISEQATTRKGKKSDRKERISEQTYQLSRWTPLVKDIMEDAIEDKLDPKQYPYISTRTVSSKTTTATSARYGNWHKNKSPGEIRNGPRIIVFIVGGVTYSEMRCVYEVTQANGKWEALIGSTHTITPVKYL 580          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Zebrafish
Match: stxbp1b (syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-GENE-060531-166])

HSP 1 Score: 111.694 bits (278), Expect = 1.857e-25
Identity = 123/564 (21.81%), Postives = 258/564 (45.74%), Query Frame = 3
Query:  141 MKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLL---EKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNL--LLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD----------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            ++VL++D+ ++++V+S   M++IM   + + E IT  RE L  +  I  + P+  +V  L    ++ RNP Y+   + F+ ++      LL+++   + +K + E    F P +  +F ++  +           + +   L R  + I +L   LK +P +RY    ++++ +A+ L +KL  + + +  L E      S LLI+DR  DPV+PLL++ T+QAM  +LL ++N+        + +  + E+ L  D D+ +    + +  E+ + +   +++   +K  N  +  +++++   ++  P ++K  +K S H+  ++E      +  + ++   EQ++      +       ++ I  +L ++ +   D IR++LLY         K   +  NL  LL+ + +   +  II  +   G    P + ++     G  S+  + I       ++  PL+ +I     E KL+   +PYIS  + S+   T                       +I+F+VGGVT+ E   ++ + Q       ++G T  +    +L
Sbjct:    6 LQVLVVDKLSMRMVSSCCKMTDIMSEGITIVEDITKKREPLPTMEAIFLITPSDESVEGLINDFRDPRNPIYRGAHVFFTDAIPDSLFNLLSKSRASKSIKTLTEIHIAFLPYESQVFSVDKSDAFQDFYSPFKADVKNQVLERLAEQIATLCATLKEYPAVRYRGEYKDNAVLAQMLQDKLDGYKADDPTLGEGPDKARSQLLILDRGFDPVSPLLHELTFQAMAYDLLPIENDVYMYETSGMGDTRMKEVLLDED-DDLWMTLRHKHIAEVSTAVTKSLKDFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHL-HLAEDCMKQYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDATVTIMDKIRIILLYIF------LKNGVSEENLCKLLQHAQIPPEDSDIISNMAHMG---VPIISEQATTRKGKKSDRKERISEQTYQLSRWTPLVKDIMEDAIEDKLDPKQYPYISTRTVSSKTTTATSARYGNWHKNKSPGEIRNGPRIIVFIVGGVTYSEMRCVYEVTQANGKWEALIGSTHTITPVKYL 558          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Zebrafish
Match: stxbp2 (syntaxin binding protein 2 [Source:ZFIN;Acc:ZDB-GENE-040426-2482])

HSP 1 Score: 110.153 bits (274), Expect = 6.352e-25
Identity = 134/592 (22.64%), Postives = 263/592 (44.43%), Query Frame = 3
Query:   90 EAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPH--YKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCD--------PFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSE----VARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIV--NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIP-----SNLFKSI----KGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCSP-----------------SNHKYTD------LIIFVVGGVTFEESLAIHNINQQISGK-NVILGGTQILNYHSFL 1697
            E I S +I  +K D +  KVL++D  ++++++S   MS+IM   V + E I+  RE +  L  I  + P  ++V+ L  + R     YK+  + F+ +        +  +   +++K ++E    F P +        P  FH        N + +   +    + I +L   L  +P IRY+   +    +A  + ++L  H +    + E      S LLI+DR  DPV+P+L++ T+QAM  +LL+++ +        I E    E+ L  D DE + Q  + +  ++   +   +    +         +I+D+   ++  P ++K  +  S H+  +++      KN L ++ E EQ++   +    +     ++ I  +L N+EIK +D IR++LLY                 +  K+ G+ E   + +  L+++ +++        +  LG P     +N  KS+    +  E+ Y  ++  P++ ++   + + K +   +P++S  +P                  N   T+      LIIFV+GGVT  E    + + +   GK  V++G + I+   SFL
Sbjct:   12 EKILSGVIKSVKKDGD-WKVLIVDHISMRILSSCCKMSDIMAEGVTIVEDISKRREPIPSLEAIYLISPVPKSVHALIADFRETAFTYKAAHVFFTDTCPDGLFAEIGRSRVAKVIKTLKEINVAFIPYESQVFTLDNPSSFHAFYSPTQTNLDDKSRMMEAMAEQIATLCDTLTEYPAIRYRLGPKENFSLAEMVMDRLNAHKADNPRMGEGTDKARSQLLIVDRGYDPVSPILHELTFQAMAYDLLDIEQDIYRYQTAGIGEARDKEVLLDED-DELWVQLRHMHIADVTKKVTELLRVFCDSKRMNTDKANIKDLSQMLKKMPQYQKELSLYSTHL-NLADACMKKFKNTLDKLCEVEQDLAMGSDAQGEPLKDAMKSIVPVLLNTEIKDYDKIRIILLY-----------------IFHKKKGIGE---ENLTKLIQHANVQQDKNIITNLQHLGCPIITGGANSGKSLPDRKERTESTYQLSRWIPVLKDVMENIIDDKCDRKQWPFLSEPAPITTTATTVSSARFGHWHKNKSATEYRSGPRLIIFVIGGVTHSEMRCAYEVTRATEGKWEVLIGSSHIITPTSFL 580          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Xenopus
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:779959])

HSP 1 Score: 419.468 bits (1077), Expect = 8.939e-140
Identity = 244/555 (43.96%), Postives = 368/555 (66.31%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXX-XXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGS 1712
            MN+  A+K Y+  MI++   GMKVL++D+ET  VV+ V++ SEI++ +VYL ERI S  RE++ +L  IC+LRPT  NV  L KELR P Y  Y ++FS+ +SK  +K LAEAD  E+V  +QE + D+   +P +F LN+      R W+   L RT  G+T+LLL+LK  P IRYQ SS++A+RLAE ++  I++E  LFE ++     LLLI+DR  D +TPLLNQWTYQAM++E+L ++NN +++        ++ E+ LS ++DEFY  N+Y NFGEI +NIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  L+ VSE EQ +      ++H++ +Q +K LL N  + + DA RLV+LYAL Y+RH     S+L+N  L+  GVSE   +++  ++EYG   ++  +LF  K +++ I    FK +KGVEN+YTQHQP + +  +Q+ + KL + L+PY+ G +    +  D+I+F+VGG T+EE+ A++N+N+   G  ++LGGT I N  SFL EV S
Sbjct:    1 MNVVLAVKQYVSKMIEDSGPGMKVLLMDKETTSVVSMVYTQSEILQKEVYLFERIDSTNRESMKHLKAICFLRPTKENVEYLIKELRRPKYSVYFLYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHVFSLNIVGCYQGRNWDAVHLYRTTQGLTALLLSLKKCPMIRYQLSSDMAKRLAEGVKQVITKEYELFEFRRTEVPPLLLILDRSDDAITPLLNQWTYQAMVHEMLGINNNRIDLSRVPGISKDLKEVVLSAENDEFYANNMYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERHLMEVSEVEQELACQ---NDHSNALQNVKRLLQNQRLAELDATRLVMLYALHYERHSSNALSSLLN-DLRVRGVSEKYRRLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFFKGLKGVENVYTQHQPFLLDTLDQLIKGKLKDNLYPYL-GPNTLRDRPQDIIVFMVGGATYEEAFAVYNLNRTTPGVRIVLGGTAIHNTKSFLEEVQS 549          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Xenopus
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:779959])

HSP 1 Score: 411.764 bits (1057), Expect = 6.116e-137
Identity = 239/548 (43.61%), Postives = 363/548 (66.24%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXX-XXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHS 1691
            MN+  A+K Y+  MI++   GMKVL++D+ET  VV+ V++ SEI++ +VYL ERI S  RE++ +L  IC+LRPT  NV  L KELR P Y  Y ++FS+ +SK  +K LAEAD  E+V  +QE + D+   +P +F LN+      R W+   L RT  G+T+LLL+LK  P IRYQ SS++A+RLAE ++  I++E  LFE ++     LLLI+DR  D +TPLLNQWTYQAM++E+L ++NN +++        ++ E+ LS ++DEFY  N+Y NFGEI +NIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  L+ VSE EQ +      ++H++ +Q +K LL N  + + DA RLV+LYAL Y+RH     S+L+N  L+  GVSE   +++  ++EYG   ++  +LF  K +++ I    FK +KGVEN+YTQHQP + +  +Q+ + KL + L+PY+ G +    +  D+I+F+VGG T+EE+ A++N+N+   G  ++LGGT I N  S
Sbjct:    1 MNVVLAVKQYVSKMIEDSGPGMKVLLMDKETTSVVSMVYTQSEILQKEVYLFERIDSTNRESMKHLKAICFLRPTKENVEYLIKELRRPKYSVYFLYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHVFSLNIVGCYQGRNWDAVHLYRTTQGLTALLLSLKKCPMIRYQLSSDMAKRLAEGVKQVITKEYELFEFRRTEVPPLLLILDRSDDAITPLLNQWTYQAMVHEMLGINNNRIDLSRVPGISKDLKEVVLSAENDEFYANNMYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERHLMEVSEVEQELACQ---NDHSNALQNVKRLLQNQRLAELDATRLVMLYALHYERHSSNALSSLLN-DLRVRGVSEKYRRLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFFKGLKGVENVYTQHQPFLLDTLDQLIKGKLKDNLYPYL-GPNTLRDRPQDIIVFMVGGATYEEAFAVYNLNRTTPGVRIVLGGTAIHNTKS 542          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Xenopus
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:779959])

HSP 1 Score: 386.726 bits (992), Expect = 1.715e-127
Identity = 232/545 (42.57%), Postives = 351/545 (64.40%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXX-XXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILN 1682
            MN+  A+K Y+  MI++   GMKVL++D+ET  VV+ V++ SEI++ +VYL ERI S  RE++ +L  IC+LRPT  NV  L KELR P Y  Y ++FS+ +SK  +K LAEAD  E+V  +Q                        R W+   L RT  G+T+LLL+LK  P IRYQ SS++A+RLAE ++  I++E  LFE ++     LLLI+DR  D +TPLLNQWTYQAM++E+L ++NN +++        ++ E+ LS ++DEFY  N+Y NFGEI +NIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  L+ VSE EQ +      ++H++ +Q +K LL N  + + DA RLV+LYAL Y+RH     S+L+N  L+  GVSE   +++  ++EYG   ++  +LF  K +++ I    FK +KGVEN+YTQHQP + +  +Q+ + KL + L+PY+ G +    +  D+I+F+VGG T+EE+ A++N+N+   G  ++LGGT I N
Sbjct:    1 MNVVLAVKQYVSKMIEDSGPGMKVLLMDKETTSVVSMVYTQSEILQKEVYLFERIDSTNRESMKHLKAICFLRPTKENVEYLIKELRRPKYSVYFLYFSNVISKSDVKSLAEADEQEVVAEVQ-----------------------GRNWDAVHLYRTTQGLTALLLSLKKCPMIRYQLSSDMAKRLAEGVKQVITKEYELFEFRRTEVPPLLLILDRSDDAITPLLNQWTYQAMVHEMLGINNNRIDLSRVPGISKDLKEVVLSAENDEFYANNMYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERHLMEVSEVEQELACQ---NDHSNALQNVKRLLQNQRLAELDATRLVMLYALHYERHSSNALSSLLN-DLRVRGVSEKYRRLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFFKGLKGVENVYTQHQPFLLDTLDQLIKGKLKDNLYPYL-GPNTLRDRPQDIIVFMVGGATYEEAFAVYNLNRTTPGVRIVLGGTAIHN 516          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Xenopus
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:779959])

HSP 1 Score: 382.874 bits (982), Expect = 1.664e-126
Identity = 219/544 (40.26%), Postives = 337/544 (61.95%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAK-IDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILN 1682
            MN+  A+K Y+  MI++   GMKVL++D+ET  VV+ V++ SEI++ +VYL ERI S  RE++ +L  IC+LRPT  NV  L KELR P Y  Y ++FS+ +SK  +K LAEAD  E+V  +QE + D+   +P +F LN+      R W+   L RT  G+T+LLL+LK  P IRYQ SS++A+RLAE ++                                    WTYQAM++E+L ++NN +++        ++ E+ LS ++DEFY  N+Y NFGEI +NIK  ME+ Q K     + ++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  L+ VSE EQ +      ++H++ +Q +K LL N  + + DA RLV+LYAL Y+RH     S+L+N L +  GVSE   +++  ++EYG   ++  +LF  K +++ I    FK +KGVEN+YTQHQP + +  +Q+ + KL + L+PY+ G +    +  D+I+F+VGG T+EE+ A++N+N+   G  ++LGGT I N
Sbjct:    1 MNVVLAVKQYVSKMIEDSGPGMKVLLMDKETTSVVSMVYTQSEILQKEVYLFERIDSTNRESMKHLKAICFLRPTKENVEYLIKELRRPKYSVYFLYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHVFSLNIVGCYQGRNWDAVHLYRTTQGLTALLLSLKKCPMIRYQLSSDMAKRLAEGVK------------------------------------WTYQAMVHEMLGINNNRIDLSRVPGISKDLKEVVLSAENDEFYANNMYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERHLMEVSEVEQELACQ---NDHSNALQNVKRLLQNQRLAELDATRLVMLYALHYERHSSNALSSLLNDL-RVRGVSEKYRRLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFFKGLKGVENVYTQHQPFLLDTLDQLIKGKLKDNLYPYL-GPNTLRDRPQDIIVFMVGGATYEEAFAVYNLNRTTPGVRIVLGGTAIHN 502          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Xenopus
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:779959])

HSP 1 Score: 357.836 bits (917), Expect = 3.732e-116
Identity = 224/545 (41.10%), Postives = 337/545 (61.83%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXX-XXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILN 1682
            MN+  A+K Y+  MI++   GMKVL++D+ET  VV+ V++ SEI++ +VYL ERI S  RE++ +L  IC+LRPT  NV  L KELR P Y  Y               L E               + Y C    F          R W+   L RT  G+T+LLL+LK  P IRYQ SS++A+RLAE ++  I++E  LFE ++     LLLI+DR  D +TPLLNQWTYQAM++E+L ++NN +++        ++ E+ LS ++DEFY  N+Y NFGEI +NIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  L+ VSE EQ +      ++H++ +Q +K LL N  + + DA RLV+LYAL Y+RH     S+L+N  L+  GVSE   +++  ++EYG   ++  +LF  K +++ I    FK +KGVEN+YTQHQP + +  +Q+ + KL + L+PY+ G +    +  D+I+F+VGG T+EE+ A++N+N+   G  ++LGGT I N
Sbjct:    1 MNVVLAVKQYVSKMIEDSGPGMKVLLMDKETTSVVSMVYTQSEILQKEVYLFERIDSTNRESMKHLKAICFLRPTKENVEYLIKELRRPKYSVY--------------FLCE---------------NMYICISTTFP-----TWRGRNWDAVHLYRTTQGLTALLLSLKKCPMIRYQLSSDMAKRLAEGVKQVITKEYELFEFRRTEVPPLLLILDRSDDAITPLLNQWTYQAMVHEMLGINNNRIDLSRVPGISKDLKEVVLSAENDEFYANNMYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERHLMEVSEVEQELACQ---NDHSNALQNVKRLLQNQRLAELDATRLVMLYALHYERHSSNALSSLLN-DLRVRGVSEKYRRLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFFKGLKGVENVYTQHQPFLLDTLDQLIKGKLKDNLYPYL-GPNTLRDRPQDIIVFMVGGATYEEAFAVYNLNRTTPGVRIVLGGTAIHN 505          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Mouse
Match: Vps45 (vacuolar protein sorting 45 [Source:MGI Symbol;Acc:MGI:891965])

HSP 1 Score: 424.476 bits (1090), Expect = 6.163e-142
Identity = 243/553 (43.94%), Postives = 360/553 (65.10%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSN-QAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV  L +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RL E ++  IS+E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+ Q K    Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q +K LL N ++ + DA+RLV+LYAL Y+RH       L+ + L+  GV+E   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+F++GG T+EE+L ++N+N+   G  ++LGGT I N  SFL EV
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVEYLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLGECVKQVISKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKRPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNVKRLLQNPKVTEFDAVRLVMLYALHYERHSSNSLPGLI-VDLRSKGVAEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFIIGGATYEEALTVYNLNRTTPGVRIVLGGTTIHNTKSFLEEV 547          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Mouse
Match: Stxbp2 (syntaxin binding protein 2 [Source:MGI Symbol;Acc:MGI:107370])

HSP 1 Score: 118.627 bits (296), Expect = 1.221e-27
Identity = 142/586 (24.23%), Postives = 267/586 (45.56%), Query Frame = 3
Query:   90 EAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNP---HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINR--EWEISSLSRTIDG----ITSLLLALKIFPQIRYQNSSEVARRLA----EKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISE-----INLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGI-PSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCSP--------SNHKY---------------TDLIIFVVGGVTFEESLAIHNINQQISGK-NVILGGTQILNYHSFL 1697
            E I S +I  +K D    KVL++D  ++++++S   MS+I+   + + E I   RE +  L  I  L PT ++V  L  + +      YK+  I F+ +  +     L  +   + VK ++E    F P +  +F L+  +   N    +      R +D     I +L   L+ +P IRY+   E   +LA     KL    +   +L E  +   S LLI+DR  DPV+PLL++ T+QAM  +LL+++ +        +SE     + L  D D   E  H ++ +   ++   +K F E  +  ++++A   +I+D+   ++  P ++K   K S H+  +++      K  + ++   EQ++      +       +++I  +L ++ +  +D IR++LLY L  +    +  + L+     QS  S     +IR L + G   +        NS G   S+  +  + +E  Y  ++  P+I ++   V E +L+  L+P++S  +P        S+ ++                 LI+++VGGV   E  A + + +   GK  V++G + IL    FL
Sbjct:   12 EKILSGVIRSVKKDGE-WKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFQGTPTFTYKAAHIFFTDTCPEPLFSELGRSRLAKAVKTLKEIHLAFLPYEAQVFSLDAPHSTYNLYCPFRAGERGRQLDALAQQIATLCATLQEYPSIRYRKGPEDTAQLAHAVLAKLNAFKADTPSLGEGPEKTRSQLLIMDRAADPVSPLLHELTFQAMAYDLLDIEQDTYRYETTGLSESREKAVLLDEDDDLWVELRHMHIADVSKKVTELLKTFCES-KRLTTDKA---NIKDLSHILKKMPQYQKELNKYSTHL-HLADDCMKHFKGSVEKLCSVEQDLAMGSDAEGEKIKDAMKLIVPVLLDASVPPYDKIRVLLLYILLRNGVSEENLAKLIQHANVQSYSS-----LIRNLEQLGGTVT--------NSAGSGTSSRLERRERMEPTYQLSRWSPVIKDVMEDVVEDRLDRKLWPFVSDPAPVPSSQAAVSSARFGHWHKNKAGVEARAGPRLIVYIVGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFL 578          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Mouse
Match: Stxbp2 (syntaxin binding protein 2 [Source:MGI Symbol;Acc:MGI:107370])

HSP 1 Score: 117.857 bits (294), Expect = 2.211e-27
Identity = 142/585 (24.27%), Postives = 266/585 (45.47%), Query Frame = 3
Query:   90 EAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNP---HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINR--EWEISSLSRTIDG----ITSLLLALKIFPQIRYQNSSEVARRLA----EKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISE-----INLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGI-PSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCSPS-----------NHKYTD-----------LIIFVVGGVTFEESLAIHNINQQISGK-NVILGGTQILNYHSFL 1697
            E I S +I  +K D    KVL++D  ++++++S   MS+I+   + + E I   RE +  L  I  L PT ++V  L  + +      YK+  I F+ +  +     L  +   + VK ++E    F P +  +F L+  +   N    +      R +D     I +L   L+ +P IRY+   E   +LA     KL    +   +L E  +   S LLI+DR  DPV+PLL++ T+QAM  +LL+++ +        +SE     + L  D D   E  H ++ +   ++   +K F E  +  ++++A   +I+D+   ++  P ++K   K S H+  +++      K  + ++   EQ++      +       +++I  +L ++ +  +D IR++LLY L  +    +  + L+     QS  S     +IR L + G   +        NS G   S+  +  + +E  Y  ++  P+I ++   V E +L+  L+P++S  +P             H + +           LI+++VGGV   E  A + + +   GK  V++G + IL    FL
Sbjct:   12 EKILSGVIRSVKKDGE-WKVLIMDHPSMRILSSCCKMSDILAEGITIVEDINKRREPIPSLEAIYLLSPTEKSVQALIADFQGTPTFTYKAAHIFFTDTCPEPLFSELGRSRLAKAVKTLKEIHLAFLPYEAQVFSLDAPHSTYNLYCPFRAGERGRQLDALAQQIATLCATLQEYPSIRYRKGPEDTAQLAHAVLAKLNAFKADTPSLGEGPEKTRSQLLIMDRAADPVSPLLHELTFQAMAYDLLDIEQDTYRYETTGLSESREKAVLLDEDDDLWVELRHMHIADVSKKVTELLKTFCES-KRLTTDKA---NIKDLSHILKKMPQYQKELNKYSTHL-HLADDCMKHFKGSVEKLCSVEQDLAMGSDAEGEKIKDAMKLIVPVLLDASVPPYDKIRVLLLYILLRNGVSEENLAKLIQHANVQSYSS-----LIRNLEQLGGTVT--------NSAGSGTSSRLERRERMEPTYQLSRWSPVIKDVMEDVVEDRLDRKLWPFVSDPAPVPSSQAAVSARFGHWHKNKAGVEARAGPRLIVYIVGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFL 577          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Mouse
Match: Stxbp1 (syntaxin binding protein 1 [Source:MGI Symbol;Acc:MGI:107363])

HSP 1 Score: 108.612 bits (270), Expect = 2.524e-24
Identity = 127/577 (22.01%), Postives = 256/577 (44.37%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNP---HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLN---------------LKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHN--SCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD----------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            KVL++D+ ++++++S   M++IM   + + E I   RE L  L  +  + P+ ++V+ L  + ++P    Y++  + F+ S        L ++   +++K + E    F P +  ++ L+               +KN I         L R  + I +L   LK +P +RY    ++++ +A+ + +KL  + + +  + E      S LLI+DR  DP +P+L++ T+QAM  +LL ++N+        I E  + E+ L  D D      H+++     E+  ++K F     +K  N  +  ++ D+   ++  P ++K  +K S H+ +  +  K        ++   EQ++      +       ++ I  +L ++ +  +D IR++LLY    +      T   +N L++ + +   + +II  +   G    P +    +     P    +  +  E  Y  ++  P+I +I     E KL+   +PYIS  S ++   T                       LIIF++GGV+  E    + + Q      V++G T IL    FL+++
Sbjct:   29 KVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPI---------LERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPTMGEGPDKARSQLLILDRGFDPSSPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFS---SSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVD-KLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKN----GITEENLNKLIQHAQIPPEDSEIITNMAHLGV---PIVTDSTLRRRSKPE---RKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPTKFLMDL 582          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Mouse
Match: Stxbp1 (syntaxin binding protein 1 [Source:MGI Symbol;Acc:MGI:107363])

HSP 1 Score: 104.76 bits (260), Expect = 4.036e-23
Identity = 126/574 (21.95%), Postives = 254/574 (44.25%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNP---HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLN---------------LKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD----------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            KVL++D+ ++++++S   M++IM   + + E I   RE L  L  +  + P+ ++V+ L  + ++P    Y++  + F+ S        L ++   +++K + E    F P +  ++ L+               +KN I         L R  + I +L   LK +P +RY    ++++ +A+ + +KL  + + +  + E      S LLI+DR  DP +P+L++ T+QAM  +LL ++N+        I E  + E+ L  D D      H+++     E+  ++K F     +K  N  +  ++ D+   ++  P ++K  +K S H+  ++E      +  + ++   EQ++      +       ++ I  +L ++ +  +D IR++LLY    +      T   +N L++ + +   + +II  +   G    P +    +     P    +  +  E  Y  ++  P+I +I     E KL+   +PYIS  S ++   T                       LIIF++GGV+  E    + + Q      V++G T IL     L
Sbjct:   29 KVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPLPSLEAVYLITPSEKSVHSLISDFKDPPTAKYRAAHVFFTDSCPDALFNELVKSRAAKVIKTLTEINIAFLPYESQVYSLDSADSFQSFYSPHKAQMKNPI---------LERLAEQIATLCATLKEYPAVRYRGEYKDNALLAQLIQDKLDAYKADDPTMGEGPDKARSQLLILDRGFDPSSPVLHELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFS---SSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHL-HLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKN----GITEENLNKLIQHAQIPPEDSEIITNMAHLG---VPIVTDSTLRRRSKPE---RKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLL 579          
BLAST of Vacuolar protein sorting-associated protein 45 vs. UniProt/SwissProt
Match: sp|O08700|VPS45_RAT (Vacuolar protein sorting-associated protein 45 OS=Rattus norvegicus OX=10116 GN=Vps45 PE=1 SV=1)

HSP 1 Score: 426.017 bits (1094), Expect = 9.810e-142
Identity = 245/553 (44.30%), Postives = 361/553 (65.28%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNK-SSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV+ L +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RL E ++  IS+E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+ Q K    Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q +K LL N ++ + DA+RLV+LYAL Y+RH       L+ + L+  GV+E   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + KL E L+PY+ G S    +  D+I+FV+GG T+EE+L ++N+N+   G  ++LGGT I N  SFL EV
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDSLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLGECVKQVISKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKRPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNVKRLLQNPKVTEFDAVRLVMLYALHYERHSSNSLPGLI-VDLRSKGVAEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGKLKENLYPYL-GPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTIHNTKSFLEEV 547          
BLAST of Vacuolar protein sorting-associated protein 45 vs. UniProt/SwissProt
Match: sp|P97390|VPS45_MOUSE (Vacuolar protein sorting-associated protein 45 OS=Mus musculus OX=10090 GN=Vps45 PE=1 SV=1)

HSP 1 Score: 424.476 bits (1090), Expect = 4.311e-141
Identity = 243/553 (43.94%), Postives = 360/553 (65.10%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSN-QAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV  L +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RL E ++  IS+E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+ Q K    Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q +K LL N ++ + DA+RLV+LYAL Y+RH       L+ + L+  GV+E   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+F++GG T+EE+L ++N+N+   G  ++LGGT I N  SFL EV
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVEYLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLGECVKQVISKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKRPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNVKRLLQNPKVTEFDAVRLVMLYALHYERHSSNSLPGLI-VDLRSKGVAEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFIIGGATYEEALTVYNLNRTTPGVRIVLGGTTIHNTKSFLEEV 547          
BLAST of Vacuolar protein sorting-associated protein 45 vs. UniProt/SwissProt
Match: sp|Q9NRW7|VPS45_HUMAN (Vacuolar protein sorting-associated protein 45 OS=Homo sapiens OX=9606 GN=VPS45 PE=1 SV=1)

HSP 1 Score: 417.157 bits (1071), Expect = 3.034e-138
Identity = 244/553 (44.12%), Postives = 361/553 (65.28%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXX-XXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  RE + +L  IC+LRPT  NV+ + +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + D+   +P LF LN+      R W+ + LSRT  G+T+LLL+LK  P IRYQ SSE A+RLAE ++  I++E  LFE ++     LL+I  R  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  N+Y NF EI SNIK  ME+  + K   Q K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++++  LL VSE EQ +      ++H+S +Q IK LL N ++ + DA RLV+LYAL Y+RH       LM + L+  GVSE   +++  ++EYG   ++  +LF  K +++ I     K +KGVEN+YTQHQP +    + + + +L E L+PY+ G S    +  D+I+FV+GG T+EE+L ++N+N+   G  ++LGGT + N  SFL EV
Sbjct:    1 MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVDYIIQELRRPKYTIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWDPAQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRCDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKNLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQ---NDHSSALQNIKRLLQNPKVTEFDAARLVMLYALHYERHSSNSLPGLM-MDLRNKGVSEKYRKLVSAVVEYGGKRVRGSDLFSPK-DAVAITKQFLKGLKGVENVYTQHQPFLHETLDHLIKGRLKENLYPYL-GPSTLRDRPQDIIVFVIGGATYEEALTVYNLNRTTPGVRIVLGGTTVHNTKSFLEEV 547          
BLAST of Vacuolar protein sorting-associated protein 45 vs. UniProt/SwissProt
Match: sp|Q54GE3|VPS45_DICDI (Vacuolar protein sorting-associated protein 45 OS=Dictyostelium discoideum OX=44689 GN=vps45 PE=1 SV=1)

HSP 1 Score: 349.362 bits (895), Expect = 5.691e-112
Identity = 216/559 (38.64%), Postives = 354/559 (63.33%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINR--EWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEG-----EISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIV-NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQS--GVSEMEIQIIRILMEY--GSIKSPNLFKEKINSLGIPSNLFK-SIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGK-NVILGGTQILNYHSFLLEVGS 1712
            M++  +I+ YI N I  +  GMKVL+LD+ET  +V+ V++ SEI++ +V+L E+I + +E +L++  + ++RPT  N+  +  EL++P +  Y + F++++SK  L  +A+AD  ++V  IQE F DF+  +P  F LNL  ++  +   W+   + R +DG+ S LLALK  P IRY ++S+  R LAEK+   ++R+ +LF+ ++    LLLI+DR+ DP+TPLL+QWTYQAMI+ELL ++NN V++ +      E+ E+ LS DHD FY +NLY NFG++ ++IK  +++ Q+K +    I +I+DMK FIENYP+F+K S  VS+HV ++ E++++I+ + L+ VSE +Q +  NH    +HNS    + +++ +S+    D + LVLLY+++Y      E   +  L  K S  G+   EI +I  L  Y   S++  +L   K N      ++ K  ++GV NIYTQH+PL+ +I + + + KL E  +PY+S  + S  +  D+IIF+VGG+T+EE+L ++  N   +G   V+LGGT ILN   FL ++ S
Sbjct:    6 MDVIASIQEYI-NKILTNIQGMKVLVLDKETAGIVSMVYTQSEILQKEVFLFEKIENTKEKMLHMKGVYFIRPTQENIQSICDELKDPKFNKYHLFFTNTISKVSLDEIAKADEQDVVSEIQEYFGDFFAVNPDTFTLNLPGMLTKKSPRWQ-GDVGRVVDGLFSSLLALKKKPVIRYSSNSDTTRYLAEKITERMNRDRDLFDFRRQGEPLLLILDRKDDPITPLLHQWTYQAMIHELLTINNNRVSLAKAPGIKDELKEVVLSLDHDIFYKENLYKNFGDLGASIKDLVDQFQDKMNTNQNIQTIDDMKKFIENYPNFQKFSTTVSKHVSLMDELNRLISLDNLMEVSEVQQELACNH----DHNSIYNHVLEIVNDSKYTDRDKLVLVLLYSIRY------EDGRVWELKEKLSSIGIPPKEIGLIDTLRGYAGASLREGDLLGTK-NIFSFARSVVKRGLQGVSNIYTQHKPLLHDILDSILKNKLKETSYPYLS-TTQSRERPQDVIIFMVGGITYEEALTVYTFNSLNTGVCRVVLGGTSILNREQFLEDLSS 550          
BLAST of Vacuolar protein sorting-associated protein 45 vs. UniProt/SwissProt
Match: sp|O49048|VPS45_ARATH (Vacuolar protein sorting-associated protein 45 homolog OS=Arabidopsis thaliana OX=3702 GN=VPS45 PE=1 SV=2)

HSP 1 Score: 310.071 bits (793), Expect = 8.204e-97
Identity = 205/560 (36.61%), Postives = 339/560 (60.54%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITS---PRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKN---VIINREWEISSL----SRTIDGITSLLLALKIFPQIRYQNSSEVARRLA-EKLRIHISREANLFESKQNXXX-XXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-GEIS-----EINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSI--KSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            M L  +++ YI  M++ D +GMKVL+LD ET+  V+ V+S SE+++ +V+L E I S    +E++ +L  + ++RPT  N+  L  +L NP +  Y + FS+ L    + +LA++D  E+V+ +QE +ADF   DP+ F LN+ +    +I    + S L     R +DGI ++ LALK  P IRYQ +S+ A+R+A E  ++    E+ LF+ ++  SS LLL+IDRR DPVTPLLNQWTYQAM++EL+ + +N V++   G +      E+ LS + D F+  N+Y NFG+I  NIK  +++ Q  + +   I ++EDM  F++NYP ++KM   VS+HV +++E+SK++   KL+ VS+ EQ++  +       +  + + DLL N  +   D +RLV+LYAL+Y++    +   L N L  +S   + +  +++ L++   +  ++ +LF  + + L I  N+ + +KGVEN+YTQHQPL+      +   +L +V +P++ G      +  +++IF+VGG T+EES ++   N   SG   ILGGT +LN   FL
Sbjct:    1 MVLVTSVRDYINRMLQ-DISGMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDSISVSKESMSHLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQEYYADFVSGDPYHFTLNMASNHLYMIPAVVDPSGLQRFSDRVVDGIAAVFLALKRRPVIRYQRTSDTAKRIAHETAKLMYQHESALFDFRRTESSPLLLVIDRRDDPVTPLLNQWTYQAMVHELIGLQDNKVDLKSIGSLPKDQQVEVVLSSEQDAFFKSNMYENFGDIGMNIKRMVDDFQQVAKSNQNIQTVEDMARFVDNYPEYKKMQGNVSKHVTLVTEMSKLVEARKLMTVSQTEQDLACN---GGQGAAYEAVTDLLNNESVSDIDRLRLVMLYALRYEKENPVQLMQLFNKLASRS--PKYKPGLVQFLLKQAGVEKRTGDLFGNR-DLLNIARNMARGLKGVENVYTQHQPLLFQTMESITRGRLRDVDYPFV-GDHFQQGRPQEVVIFMVGGTTYEESRSVALQNATNSGVRFILGGTAVLNSKRFL 552          
BLAST of Vacuolar protein sorting-associated protein 45 vs. TrEMBL
Match: A0A210QI60 (Vacuolar protein sorting-associated protein 45 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT14788 PE=3 SV=1)

HSP 1 Score: 475.707 bits (1223), Expect = 9.441e-159
Identity = 262/557 (47.04%), Postives = 378/557 (67.86%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERI-TSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIH-----EGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKS--PNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIG 1721
            MN+  A+K YI  MI     GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI +S RE + +L CIC+LRPT  NV LL +ELR P Y  Y I+FS+ +SKQ +K+LAEAD  E+V+ +QE F DF   +P LF LN+        W  SSL+RT  G+T++LL+LK  P IRYQNSSE+ARRLAE +R  IS+EA LFE  K + + +LL++DRR DPVTPLLNQWTYQAM++ELL ++NN +N+        ++ E+ LS +HDEFY  N+YNNFGEI +NIK  MEE Q KS +QAK++SI DMK FIE YP F+KMS  V++HV ++ E+S++++K  LL VSE EQ +       +H++C+Q IK LL   +  + D +RLV+LYAL+Y+ H   + S L+   L++ G+SE    ++  +++YG  K+   +LF  + + + I     K +KGVEN+YTQH+PL+ +I +Q+ + KL E  +PY+ G      K  D+++F++GG T+EE+LA+H +N+   G  ++LGGT + N+ SFL EV   +G
Sbjct:    1 MNVILAVKQYISKMIDESGPGMKVLLMDKETTSIVSMVYAQSEILQKEVYLFERIDSSARETMKHLKCICFLRPTRDNVELLAQELRMPKYGLYYIYFSNVISKQDVKVLAEADDQEVVREVQEFFGDFIAVNPHLFTLNILGCSQALNWSPSSLTRTTQGLTAVLLSLKKCPMIRYQNSSEMARRLAENVRSTISKEAALFEFRKTDVAPVLLVLDRRDDPVTPLLNQWTYQAMVHELLGINNNRINLAHIPGVTKDLQEVVLSAEHDEFYANNMYNNFGEIGTNIKDLMEEFQKKSKSQAKVESIADMKAFIETYPQFKKMSGAVAKHVTVVGELSRLVSKINLLEVSETEQELACQ---GDHSACLQKIKGLLNGDKTPQLDMLRLVMLYALRYESHSNNDVSGLVE-ALRRKGLSEKHRSMVHAVLQYGGRKARGSDLFGNQ-DPVAITKRFLKGLKGVENVYTQHKPLMYSILDQLIKGKLKEGAYPYL-GTGQLRDKPQDIVVFMIGGATYEEALAVHTLNRSSPGVRIVLGGTTVHNFKSFLDEVSQAVG 551          
BLAST of Vacuolar protein sorting-associated protein 45 vs. TrEMBL
Match: R7TQD9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_126905 PE=3 SV=1)

HSP 1 Score: 461.84 bits (1187), Expect = 1.306e-153
Identity = 255/547 (46.62%), Postives = 373/547 (68.19%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITS-PRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIH-----EGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHS 1691
            MN+  A+K Y+  MI+    GMKVL++D+ET  +V+ V++ SEI++ +VYL ER+ S  RE++ +L CIC+LRPT  NV L+ +ELR+P Y  Y I+ S+ +SKQ +K LAEAD  E+V+ +QE + DF      LF LNL  +   R W    L+RT+ G+T+LLL+LK  P IRYQNSSE+A+RLA+ +R  ISREA LF+  + +   LLL++DRR DPVTPLLNQWTYQAM++ELL ++NN +N+        ++ E+ LS +HD+FY  N+Y NFGEI SNIK  MEE Q KS +QAK++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++ K+ L+ VSECEQ +V      +H+  +Q IK L+ N + ++ DA+RLV+LYAL+Y++H   + + LM   L++ GVSE   +++  ++EY    ++  +LF E    + +     K +KGVENIY QH+PL+ NI +QV   KL E  +PY+ G S    +  D+I+F++GG T EE++A+HN+N+Q  G  ++LGG+ I N +S
Sbjct:    1 MNVILAVKQYVSKMIEESGPGMKVLLMDKETTSIVSMVYAQSEILQKEVYLFERLDSVGRESMKHLKCICFLRPTRENVELMAQELRHPKYGLYYIYLSNVISKQDVKSLAEADDQEVVREVQEYYGDFVAVGVHLFSLNLSGICQGRSWVNPQLNRTVQGLTALLLSLKKCPMIRYQNSSEMAKRLADNVRQVISREAALFDFRRTDVPPLLLLLDRRDDPVTPLLNQWTYQAMVHELLGINNNRINLSSVPGVSRDLQEVVLSSEHDDFYSANMYLNFGEIGSNIKNLMEEFQRKSQSQAKVESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVGKHGLMDVSECEQELVCQ---SDHSQSLQKIKSLIANEKTREQDAVRLVMLYALRYEKHSSNDITALMG-ALQRKGVSERLRKMVPAVLEYAGQKVRGSDLF-ETETPIAMTKKFLKGLKGVENIYAQHKPLLHNILDQVIRSKLREASYPYL-GTSQLKDRPQDIIVFIIGGATHEEAIAVHNLNRQTPGVRIVLGGSCIHNMNS 541          
BLAST of Vacuolar protein sorting-associated protein 45 vs. TrEMBL
Match: A0A1S3GYE5 (vacuolar protein sorting-associated protein 45 OS=Lingula unguis OX=7574 GN=LOC106150430 PE=3 SV=1)

HSP 1 Score: 462.225 bits (1188), Expect = 1.695e-153
Identity = 245/552 (44.38%), Postives = 378/552 (68.48%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERI-TSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIH-----EGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MN+  A+K Y+  M+ +   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI +S RE + +L C+C+LRPT  NV+LL++ELR+P Y  Y I+FS+ +S+Q +K+LAEAD  E+V+ +QE + D+    P LF  N+        W   +L+R++ G+T++LL+LK  P IR+QNSSE+ARR+A+ +R  IS+EA+LF+  + +   +LLI+DRR DPVTPLLNQWTYQAM++ELLE+ NN +++        E+ E+ LS +HDEFY  N+Y NFGEI  NIK  MEE Q KS  Q+K++SI+DMK F+ENYP F+KMS  VS+HV ++ E+S+++  + LL VSE EQ +V      +H+  +Q I+ LL N  ++  D +RLV+LYAL++++H   + + L + L K+ GVSE   +++  +++Y    ++S +LF  K +++      FK +KGVEN+YTQH  L+  + +Q+ + +L E  +PY+ G +    +  D+I+F++GG T+EE+LA+HN+N+ I G  ++LGGT I N  SFL E+
Sbjct:    1 MNVVLAVKQYVTKMVDDSGQGMKVLLMDKETTSIVSMVYAQSEILQKEVYLFERIDSSARETMKHLKCVCFLRPTKENVDLLQQELRSPKYGLYFIYFSNVISRQDVKVLAEADDQEVVREVQEFYGDYVAVSPHLFSFNIVGHCQGNNWVTPALTRSVQGLTAVLLSLKKCPMIRFQNSSEMARRMADNVRQVISKEASLFDFRRTDVPPVLLILDRRDDPVTPLLNQWTYQAMVHELLEISNNRISLASVPGISKELKEVVLSAEHDEFYANNMYANFGEIGVNIKTLMEEFQKKSQGQSKVESIQDMKAFLENYPQFKKMSGTVSKHVTVVGELSRLVGAHNLLEVSETEQELVCQ---GDHSLALQKIRSLLANDNVRDIDKLRLVMLYALRFEKHSNNDVTGLADALAKK-GVSEKLRRMVGAVVDYAGSRVRSSDLFGSK-DAITFTKKFFKGLKGVENVYTQHNTLLQELLDQLIKGRLKEASYPYV-GQAQLRDRPQDIIVFMIGGATYEEALAVHNMNKTIPGVRIVLGGTHIHNMKSFLDEI 546          
BLAST of Vacuolar protein sorting-associated protein 45 vs. TrEMBL
Match: X1YZB1 (Uncharacterized protein OS=Capitella teleta OX=283909 PE=3 SV=1)

HSP 1 Score: 461.455 bits (1186), Expect = 2.246e-153
Identity = 255/547 (46.62%), Postives = 372/547 (68.01%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITS-PRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIH-----EGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHS 1691
            MN+  A+K Y+  MI+    GMKVL++D+ET  +V+ V++ SEI++ +VYL ER+ S  RE++ +L CIC+LRPT  NV L+ +ELR+P Y  Y I  S+ +SKQ +K LAEAD  E+V+ +QE + DF      LF LNL  +   R W    L+RT+ G+T+LLL+LK  P IRYQNSSE+A+RLA+ +R  ISREA LF+  + +   LLL++DRR DPVTPLLNQWTYQAM++ELL ++NN +N+        ++ E+ LS +HD+FY  N+Y NFGEI SNIK  MEE Q KS +QAK++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++ K+ L+ VSECEQ +V      +H+  +Q IK L+ N + ++ DA+RLV+LYAL+Y++H   + + LM   L++ GVSE   +++  ++EY    ++  +LF E    + +     K +KGVENIY QH+PL+ NI +QV   KL E  +PY+ G S    +  D+I+F++GG T EE++A+HN+N+Q  G  ++LGG+ I N +S
Sbjct:    1 MNVILAVKQYVSKMIEESGPGMKVLLMDKETTSIVSMVYAQSEILQKEVYLFERLDSVGRESMKHLKCICFLRPTRENVELMAQELRHPKYGLYYIDLSNVISKQDVKSLAEADDQEVVREVQEYYGDFVAVGVHLFSLNLSGICQGRSWVNPQLNRTVQGLTALLLSLKKCPMIRYQNSSEMAKRLADNVRQVISREAALFDFRRTDVPPLLLLLDRRDDPVTPLLNQWTYQAMVHELLGINNNRINLSSVPGVSRDLQEVVLSSEHDDFYSANMYLNFGEIGSNIKNLMEEFQRKSQSQAKVESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVGKHGLMDVSECEQELVCQ---SDHSQSLQKIKSLIANEKTREQDAVRLVMLYALRYEKHSSNDITALMG-ALQRKGVSERLRKMVPAVLEYAGQKVRGSDLF-ETETPIAMTKKFLKGLKGVENIYAQHKPLLHNILDQVIRSKLREASYPYL-GTSQLKDRPQDIIVFIIGGATHEEAIAVHNLNRQTPGVRIVLGGSCIHNMNS 541          
BLAST of Vacuolar protein sorting-associated protein 45 vs. TrEMBL
Match: A0A433T7Z4 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_014598 PE=3 SV=1)

HSP 1 Score: 461.84 bits (1187), Expect = 2.790e-153
Identity = 260/556 (46.76%), Postives = 373/556 (67.09%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERI-TSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIH-----EGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIK--SPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWI 1718
            MN+  A+K YI  MI+    GMKVL++D ET  +V+ V++ SEI++ +VYL ERI TS RE + +LNCI YLRPT  N++LL +ELR P Y  Y I+FS+ +SKQ +K+LAEAD  E+V+ +QE + D+   +P +F LNL     N  W    LSRTI G+TS+LL+LK  P IRYQNSSE+ARRLA+ +R  I +EA LFE  K +   +LLI+DRR D VTPLLNQWTYQAM++ELL ++NN +N+        ++ E+ LS +HDEFY  N+Y NFGEI SNIK  MEE Q KS + AK++SI DMK F+E YP F+KMS  VS+HV I+ E+S+++  + LL VSE EQ I       +H++ +Q I+ LL + +++  D +RLV+LYAL+Y+ H   + + L++  L+Q GVS     +++ +++YG  K    +LF  + +++ I   L K +KGVEN+YTQH+PL++ + + + + KL E  +PY+ G      +  D+I+F++GG T+EESLA+H  N+ + G  ++LGGT I N  SFL EV   I
Sbjct:    1 MNVILAVKQYISKMIEGCGGGMKVLLMDRETTSIVSMVYAQSEILQKEVYLFERIDTSGRETMKHLNCIVYLRPTRENIDLLAQELRMPKYGLYFIYFSNVISKQDVKILAEADDQEVVREVQEFYGDYIAVNPHVFSLNLVGCCQNLSWSPPVLSRTIQGLTSVLLSLKKCPMIRYQNSSEMARRLADSVRQVIGKEAALFEFRKTDVPPVLLILDRRDDAVTPLLNQWTYQAMVHELLSINNNRINLSNVPGITKDLQEVVLSAEHDEFYSNNMYMNFGEIGSNIKELMEEFQKKSKSHAKVESIADMKAFVETYPQFKKMSGTVSKHVTIVGELSRLVGAHLLLEVSETEQEIACQ---GDHSALLQRIRALLASDKVRHIDMLRLVMLYALRYESHSNNDLAGLVD-ALRQRGVSAEHRAMVQSILDYGGKKGRGSDLFGNQ-DAVAITKRLLKGLKGVENVYTQHKPLLTTLIDNLIKGKLKEASYPYL-GTGQLRDRPQDIIVFIIGGCTYEESLAVHTFNKTMPGVRIVLGGTCIHNSKSFLQEVSMAI 550          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Cavefish
Match: vps45 (pleckstrin homology domain-containing family O member 1-A-like [Source:NCBI gene;Acc:103034895])

HSP 1 Score: 429.483 bits (1103), Expect = 4.719e-144
Identity = 246/558 (44.09%), Postives = 371/558 (66.49%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIG 1721
            MN+  A+K Y+  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  REN+ +L  IC+LRPT  NV  L +EL  P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + DF   +P LF LNL+ V+  R WE + L R   G+TS+LLALK  P IRYQ SS++A+RLAE ++  I++E  LF+  K     LLLI+DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  NLY NFGEI +NIK  ME+  + K   Q K++SI DMK F++NYP F+KMS  VS+HV ++ E+S+++++ +L+ VSE EQ +      ++H+S  Q ++ LL N  + + DA+RLV+LYAL+Y+RH      +LM+  L + GVSE   ++++ +++YG   ++  +L     +++ I    FK +KGVEN+YTQHQPL+ +  +Q+ + +L +  FPY+ G S    +  D+I+F++GG T+EE+L ++N+N+ + G  ++LGGT I N  SFL EV S  G
Sbjct:    1 MNVTLAVKQYVSKMIESSGPGMKVLLMDKETTSIVSMVYTQSEILQKEVYLFERIDSQNRENMKHLKAICFLRPTKENVENLIQELHRPKYSVYFIYFSNVISKSEVKALAEADEQEVVAEVQEFYGDFIAVNPHLFSLNLQGVVRGRSWEPTILPRVTQGLTSVLLALKKCPMIRYQLSSDMAKRLAESVKQIITKEYELFDFRKTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGLNNNRIDLSRVPGISKDLREVVLSAENDEFYANNLYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVDNYPQFKKMSGTVSKHVTVVGELSRLVSERQLMEVSEVEQELACQ---NDHSSAQQNVRRLLQNPRVAELDAVRLVMLYALRYERHSSSMLPSLMD-ELNRRGVSEKHCKMVQAMIDYGGKRVRGTDLIT-ATDAVAITKQFFKGLKGVENVYTQHQPLLHDTLDQLIKGRLKDSQFPYL-GPSSLRDRPQDIIVFIIGGATYEEALTVYNLNRTMPGVRIVLGGTTIHNTKSFLEEVTSAAG 552          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Cavefish
Match: vps45 (pleckstrin homology domain-containing family O member 1-A-like [Source:NCBI gene;Acc:103034895])

HSP 1 Score: 385.185 bits (988), Expect = 5.741e-127
Identity = 229/545 (42.02%), Postives = 350/545 (64.22%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILN 1682
            MN+  A+K Y+  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  REN+ +L  IC+LRPT  NV  L +EL  P Y  Y I+FS+ +SK  +K LAEAD  E+V  +Q                        R WE + L R   G+TS+LLALK  P IRYQ SS++A+RLAE ++  I++E  LF+  K     LLLI+DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  NLY NFGEI +NIK  ME+  + K   Q K++SI DMK F++NYP F+KMS  VS+HV ++ E+S+++++ +L+ VSE EQ +      ++H+S  Q ++ LL N  + + DA+RLV+LYAL+Y+RH      +LM+  L + GVSE   ++++ +++YG   ++  +L     +++ I    FK +KGVEN+YTQHQPL+ +  +Q+ + +L +  FPY+ G S    +  D+I+F++GG T+EE+L ++N+N+ + G  ++LGGT I N
Sbjct:    1 MNVTLAVKQYVSKMIESSGPGMKVLLMDKETTSIVSMVYTQSEILQKEVYLFERIDSQNRENMKHLKAICFLRPTKENVENLIQELHRPKYSVYFIYFSNVISKSEVKALAEADEQEVVAEVQ-----------------------GRSWEPTILPRVTQGLTSVLLALKKCPMIRYQLSSDMAKRLAESVKQIITKEYELFDFRKTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGLNNNRIDLSRVPGISKDLREVVLSAENDEFYANNLYLNFGEIGTNIKNLMEDFQKKKPKGQQKLESISDMKAFVDNYPQFKKMSGTVSKHVTVVGELSRLVSERQLMEVSEVEQELACQ---NDHSSAQQNVRRLLQNPRVAELDAVRLVMLYALRYERHSSSMLPSLMD-ELNRRGVSEKHCKMVQAMIDYGGKRVRGTDLIT-ATDAVAITKQFFKGLKGVENVYTQHQPLLHDTLDQLIKGRLKDSQFPYL-GPSSLRDRPQDIIVFIIGGATYEEALTVYNLNRTMPGVRIVLGGTTIHN 516          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Cavefish
Match: vps45 (pleckstrin homology domain-containing family O member 1-A-like [Source:NCBI gene;Acc:103034895])

HSP 1 Score: 382.489 bits (981), Expect = 3.263e-126
Identity = 225/557 (40.39%), Postives = 345/557 (61.94%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIG 1721
            MN+  A+K Y+  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  REN+ +L  IC+LRPT  NV  L +EL  P Y  Y I+FS+ +SK  +K LAEAD  E+V  +QE + DF   +P LF LNL+ V+  R WE + L R   G+TS+LLALK  P IRYQ SS++A+RLAE ++  I++E  LF+  K     LLLI+DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY                                        F++NYP F+KMS  VS+HV ++ E+S+++++ +L+ VSE EQ +      ++H+S  Q ++ LL N  + + DA+RLV+LYAL+Y+RH      +LM+  L + GVSE   ++++ +++YG   ++  +L     +++ I    FK +KGVEN+YTQHQPL+ +  +Q+ + +L +  FPY+ G S    +  D+I+F++GG T+EE+L ++N+N+ + G  ++LGGT I N  SFL EV S  G
Sbjct:    1 MNVTLAVKQYVSKMIESSGPGMKVLLMDKETTSIVSMVYTQSEILQKEVYLFERIDSQNRENMKHLKAICFLRPTKENVENLIQELHRPKYSVYFIYFSNVISKSEVKALAEADEQEVVAEVQEFYGDFIAVNPHLFSLNLQGVVRGRSWEPTILPRVTQGLTSVLLALKKCPMIRYQLSSDMAKRLAESVKQIITKEYELFDFRKTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGLNNNRIDLSRVPGISKDLREVVLSAENDEFY-------------------------------------ANAFVDNYPQFKKMSGTVSKHVTVVGELSRLVSERQLMEVSEVEQELACQ---NDHSSAQQNVRRLLQNPRVAELDAVRLVMLYALRYERHSSSMLPSLMD-ELNRRGVSEKHCKMVQAMIDYGGKRVRGTDLIT-ATDAVAITKQFFKGLKGVENVYTQHQPLLHDTLDQLIKGRLKDSQFPYL-GPSSLRDRPQDIIVFIIGGATYEEALTVYNLNRTMPGVRIVLGGTTIHNTKSFLEEVTSAAG 514          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Cavefish
Match: stxbp2 (syntaxin binding protein 2 [Source:NCBI gene;Acc:103039197])

HSP 1 Score: 129.028 bits (323), Expect = 3.697e-31
Identity = 142/601 (23.63%), Postives = 274/601 (45.59%), Query Frame = 3
Query:   81 NLKEAIKSYIINMIKND---DNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPH--YKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPC--------DPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAE----KLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHD---EFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIP-----SNLFKSI----KGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYISGCSP-----------------SNHKYTD------LIIFVVGGVTFEESLAIHNINQQISGK-NVILGGTQILNYHSFL 1697
             LK  +   IIN +      D   KVL++D  ++++++S   MS+IM   V + E I   RE +  L  I ++ P  ++V+ L  + ++    YK+  I F+ +        + ++    ++K ++E    F P         DP L H+       N +     +    + I +L   LK +P IRY+   +    LAE    +L  H +   ++ E      S LLI+DR  DP++PLL++ T+QAM+ +LLE+D +        + E ++ E+ L  D D   +  H ++ +   ++   ++ F E  +  S+++A   +I+D+   ++  P ++K  +    H+  +++      K  L ++ E EQ++      +       ++ I  +L N+EI+ +D IR++LL+                 +  K+ G++E   + +  L+++ +++  +     +  LG P     +N  KS+    +  E+ Y  ++  P I ++   + E KL+   +P++S  +P                  N   T+      LIIFV+GGVT  E  + + + +   GK  V++G + IL   SFL
Sbjct:    5 GLKAVVGEKIINGVLKSIKKDGEWKVLIVDHISMRILSSCCKMSDIMAEGVTIVEDINKRREPISSLEAIYFISPVSKSVDGLISDFKDSAFTYKAAHIFFTENCPDGLFAKIGKSRVARVIKTLKEINVAFLPYESQVYSLDDPSLLHVVYSPTGSNEDERTKKMEAVAEQIATLCDTLKEYPAIRYRQGPKENVALAEMVLDRLNAHKADNPSMGEGTDKARSQLLIVDRGYDPISPLLHELTFQAMVYDLLEIDQDIYRYETTGVGENKMKEVLLDEDDDLWVQLRHMHIADVTKKVTEMLRSFCES-KRMSTDKA---NIKDLSQMLKKMPQYQKELSLYGTHL-NLADACMKKFKTSLDKLCEVEQDLAMGCNAEGEPIKDAMKSIVPVLLNAEIQPYDKIRIILLF-----------------IFHKKKGIAE---ENLTKLIQHANVQQDSNIITNMQHLGCPIITGGANAGKSLPDRKERTESTYQLSRWTPTIKDVMESIIEDKLDRKQWPFLSDPAPISTTQTAVSSARFGQWHKNKTATEYRSGPRLIIFVIGGVTHSEMRSAYEVTRATEGKWEVLIGSSHILTPTSFL 580          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Cavefish
Match: stxbp3 (syntaxin binding protein 3 [Source:NCBI gene;Acc:103044348])

HSP 1 Score: 112.079 bits (279), Expect = 1.425e-25
Identity = 144/580 (24.83%), Postives = 262/580 (45.17%), Query Frame = 3
Query:   90 EAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELR-NPHYKSYDIHFSHSLSKQY---LKLLAEADHFELVKNIQESF----ADFYPCD-PFLFHLNLKNVIINREWEIS-SLSRTIDGITSLLLALKIFPQIRYQNSSEV--ARRLAE----KLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEIN-LSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGN------SEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPS-----------NHK------YTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            + IK  II   K  +   K+++LD  T ++++S   MS++M   + + E +   RE +L +  + ++ PT + V+    + +  P YK+  ++F+          +KL   A H ++ K I  SF    +  + CD P  F    K++   +  E   +L    D + +L   L  +P +RY+  S++   + LAE    KL  H   + N  + K+   + L+I+DR  DPV+P+L++ TYQAM  +L+ + N+       + SE   L  + DE + +  + +  E+   I   ++E+  NK   + KI SI  +K  ++  P+FRK  ++   H+ +  +       +   ++ + EQ++     ++      Q +KD +        +    HD IR VLLY    +    +   NL  L+       E E      L+    I SP+LF  +      P    +S +   N+ ++  P+I ++   V E KL+   +P+ S C  +            HK       + LIIFV+GG+++ E    + + Q      VI+G + IL   S L ++
Sbjct:   15 QKIKDTIIADCKKSEV-WKIMILDSFTTRLLSSCCKMSDLMAEGITIVEDLFKSREPVLEMKAVYFMSPTTKCVDAFINDFKLKPKYKAAYVYFTDYCPDDLFNKMKLFC-AKHIKVCKEINISFLPLESQVFTCDNPGAF----KSIYSPQSTERGQTLETFADQLVTLCATLDEYPGVRYKKDSQLDYTKVLAELVDNKLAQHYELD-NSGKKKEKTQAQLIIVDRGFDPVSPILHELTYQAMAYDLVPIKNDIYKYKGKDGSEKEALLNESDELWIRLRHLHIAEVTEQIPKLVKEISANKKQPEGKI-SIGGLKQLMKQMPAFRKQVSQKRNHLTLAEDCMNKFQSSVE-KLCKAEQDLAVGSDVEG-----QKVKDPMRTLLPVLLNPHSTHDKIRAVLLYIFSLNGTTEE---NLTKLIQHVKIEGESEYITNWNLLGVPIISSPSLFSSRK-----PPRRDRSQEETYNL-SRWTPIIKDVMEDVVENKLDTKDWPHQSECPAAWNGSRAVSARQKHKPSAQEAGSRLIIFVLGGISYSEMRCAYEVTQATKSCEVIIGSSHILTPTSLLEDI 571          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Sea Lamprey
Match: vps45 (vacuolar protein sorting 45 homolog [Source:ZFIN;Acc:ZDB-GENE-060503-526])

HSP 1 Score: 426.787 bits (1096), Expect = 1.232e-143
Identity = 237/552 (42.93%), Postives = 362/552 (65.58%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            M++  A+K Y+  MI     GMKVL++D++T  +V+ V+S SEI++ +VYL ER+ S  RE + +L  +C++RP   ++ LL +ELR P Y  Y I+FS+ +SK  +K LAEAD  E+V+ ++E +AD+    P +F LNL      R W+   L+R   G+T++LL+LK  P IRYQ+SSE+ARRLAE ++  IS++A LF+  K +   LLL++DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS +HD+FY  N+Y NFGEI +NIK  MEE Q KS +  K++SI DMK F+ENYP F+KMS  VS+HV ++ E+S+++    LL VSE EQ +       +H+S +Q ++ +L   ++ + DA RLV+LYAL+Y+RH     S L+  L ++  VS    Q++  ++EYG   ++  +LF  + + + I    FK +KG++N+YTQHQPL+++  +QV + +L E   PY+ G S    +  D+I+F+VGG T+EE+L ++N+N+   G  VILGGT + N  SFL EV
Sbjct:    1 MDVVSAVKQYVCKMIGETGPGMKVLLMDKDTTSIVSMVYSQSEILQKEVYLFERVDSVNREPMKHLKAVCFIRPVKESIELLVQELRRPKYGLYFIYFSNVVSKSDVKTLAEADEHEVVRELKEFYADYIAISPHIFSLNLTGCSQGRSWDPVLLTRATLGLTAVLLSLKKCPVIRYQHSSELARRLAECVKQMISKDAALFDFRKTDVPPLLLVLDRNDDAITPLLNQWTYQAMVHELLGINNNRIDLSSVPGVGRDLKEVVLSSEHDDFYASNMYLNFGEIGTNIKNLMEEFQKKSKSSQKVESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVASRSLLSVSELEQELACQ---SDHSSALQSLRRMLQQPKVSELDAARLVMLYALRYERHSSNNLSGLLEDLSRRQ-VSSQYRQLVNSVLEYGGLRVRGSDLFGSQ-DPMSITKQFFKGLKGIQNVYTQHQPLLADTLDQVLKGRLKEASHPYL-GSSSLRDRPQDVIVFMVGGATYEEALTVYNLNRTNPGVRVILGGTNVHNTRSFLEEV 546          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Sea Lamprey
Match: stxbp1a (syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-GENE-050626-106])

HSP 1 Score: 94.7449 bits (234), Expect = 1.103e-20
Identity = 131/590 (22.20%), Postives = 260/590 (44.07%), Query Frame = 3
Query:   84 LKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNP---HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIIN----REWEISS--LSRTIDGITSLLLALKIFPQIRYQ----NSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDN-----NCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEEL-QNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYA-LKYD---RHPRKETSNLMNLLLKQSGVSEMEI----------QIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD----------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILN 1682
            + E I   +I  +K      KVL++D+ ++++++S   M++IM   + + E I   RE +  L  +  + P+ ++V  L  + ++     YK   + F+ S  +   K L ++   +  K +QE    F P +  +F L+      +    ++  + +  + R  + I +L   L+ +P +RY+    N++ +A+ + +KL  + +    + +      S LLIIDR  DP +P+L++ T+QAM  +LL ++N     +   + E  + E+ L  D D+ + Q  + +  ++  ++   ++E   +K     +  ++ D+   ++  P ++K  +K S H+  ++E      +  + ++   EQ++ N    +       ++ I  +L ++ +  +D IR++LLY  LK D    H  K     MN  L  S     EI            + + M Y S    +L   K++              ++   T  QP +SN+     + KL+   +PYIS  S +    T                       LI+F++GGV+F E    + + Q      V++G T IL 
Sbjct:   11 VGEKIMKEVIQKVKKKGE-WKVLVVDQLSMRMLSSCCKMTDIMTEGITIVEDINKRREPIPSLEALYLITPSEKSVRALINDFKDASIFKYKGAHVFFTDSCPEALFKELGKSRVAKNTKALQEINIAFLPYESQVFSLDAPEAFQSFYSPQKASVRNHMMERLAEQIATLCATLQEYPAVRYRGDYANNTYLAQLVQDKLDAYKADNPTMGDGPDKGRSQLLIIDRGFDPTSPVLHELTFQAMAYDLLPIENDVYKYDTSGMGENHMKEVLLDED-DDLWMQLRHKHIADVSQDVTKSLKEFSSSKRMATGEKMNMRDVSQMLKKMPQYQKELSKYSTHL-HLAEDCMKHYQGTVNKLCRVEQDLANGTDAEGEKIKDPMRAIVPILLDTNVSTYDKIRIILLYIFLKNDIINCHLHK-----MNKELAGSQPRFCEIITGKKACFCDSTVTVAMLYLS----HLHTRKMD--------------IQCHLTLGQPNMSNVCADAVDDKLDAKHWPYISARSSATFGTTAVSARYGHWHKNKSPGEYRTGPRLIVFIIGGVSFSEMRCAYEVTQANGKWEVLIGSTHILT 574          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Yeast
Match: VPS45 (Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment [Source:SGD;Acc:S000003063])

HSP 1 Score: 250.366 bits (638), Expect = 8.395e-76
Identity = 177/553 (32.01%), Postives = 301/553 (54.43%), Query Frame = 3
Query:  126 NDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPC--DPFLFHLNLKNVIINR-EWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISR-EANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIH-----EGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEI--QIIRILMEYGSIKSPNLFKEKINS---LGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYIS-------GCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGK-NVILGGTQILNYHSFLLEVGS 1712
            N+   +KVL+LD+ T   ++   + SE++K ++YL ERI +  RE   +L C+ Y++PT   +  L +ELRNP Y  Y I FS+ +SK  L+ LAE+D  E V  ++E F DF+    D F F L  +  + N+  W    L++  + + S+LL+LKI P IRY+ +S++  RLA+++   I + E   F+    + + +LLI+DR  DP+TPLL  WTYQ+MINE + +  N V++      + ++ ++ LS   D F+   +Y NFGE+   +K ++   ++K+   ++I+SIED+K FIE YP FRK+S  V++H+ I+ E+ + +    +  +SE EQN+  H    N      +IK LL N  + K+  ++L  +Y+L  ++    +   L+ +L +Q    ++    +   +      +   N  K+ I +       S +       EN+Y QH P IS++   +++  L    F  I        G   S     D+I+FV+GGVT+EE+  +H+ N  ++ +  V+LGGT IL+   ++  + S
Sbjct:   27 NEHQRIKVLLLDKNTTPTISLCATQSELLKHEIYLVERIENEQREVSRHLRCLVYVKPTEETLQHLLRELRNPRYGEYQIFFSNIVSKSQLERLAESDDLEAVTKVEEIFQDFFILNQDLFSFDLQPREFLSNKLVWSEGGLTKCTNSLVSVLLSLKIKPDIRYEGASKICERLAKEVSYEIGKNERTFFDFPVMDSTPVLLILDRNTDPITPLLQPWTYQSMINEYIGIKRNIVDLSKVPRIDKDLEKVTLSSKQDAFFRDTMYLNFGELGDKVKQYVTTYKDKTQTNSQINSIEDIKNFIEKYPEFRKLSGNVAKHMAIVGELDRQLKIKNIWEISEIEQNLSAHDA--NEEDFSDLIK-LLQNEAVDKYYKLKLACIYSLN-NQTSSDKIRQLVEILSQQLPPEDVNFFHKFKSLFSRQDKMTQSNHDKDDILTELARRFNSRMNSKSNTAENVYMQHIPEISSLLTDLSKNALFRDRFKEIDTQGHRVIGNQQSKDIPQDVILFVIGGVTYEEARLVHDFNGTMNNRMRVVLGGTSILSTKEYMDSIRS 575          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Yeast
Match: SLY1 (Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex [Source:SGD;Acc:S000002597])

HSP 1 Score: 69.3218 bits (168), Expect = 8.855e-13
Identity = 68/294 (23.13%), Postives = 130/294 (44.22%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAE----ADHFELVKNIQESFADFYPCDPFLFHLNLKNVIIN------REWEISSLSRTI-DGITSLLLALKIFPQIRYQNSSE---VARRLAEKLRIHI----SREANLFESKQNXXXXXXXXXXXXXPVTPLLN-QWTYQAMINELLEVDNNCVNI----HEGEISEINLSP---------DHDEFYHQNLYNNFGEICSNIKCFM 929
            KVL+LD ++   ++SV  +++++K  + +   I   R  L  +  I ++ PT  N++++  +L++  Y  + I+F+ SL +  L+ LA+        + +K + + + DF   +P LF L + N  +        E EI+ L   I DG+ + +L +   P IR         +A +L  KLR  +    S   +  +   +    +LII  R      + +  W YQ M+ ++ ++  N V I     E         P          +D F+ +N +  F E   N++  +
Sbjct:   54 KVLILDIKSTATISSVLRVNDLLKAGITVHSLIKQDRSPLPDVPAIYFVSPTKENIDIIVNDLKSDKYSEFYINFTSSLPRNLLEDLAQQVSITGKSDKIKQVYDQYLDFIVTEPELFSLEISNAYLTLNDPKTTEEEITGLCANIADGLFNTVLTINSIPIIRAAKGGPAEIIAEKLGTKLRDFVINTNSSSTSTLQGNDSLERGVLIILDRNIDFASMFSHSWIYQCMVFDIFKLSRNTVTIPLESKENGTDNTTAKPLATKKYDIEPNDFFWMENSHLPFPEAAENVEAAL 347          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Yeast
Match: VPS33 (ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains [Source:SGD;Acc:S000004388])

HSP 1 Score: 52.373 bits (124), Expect = 1.539e-7
Identity = 29/105 (27.62%), Postives = 57/105 (54.29%), Query Frame = 3
Query:  735 PVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHV 1049
            P+TPLL Q TY  ++++L E  N+ + I E    ++N +   D+ ++   + NFG I   +    +ELQ +   + K +S+ ++K F+++  S ++  A +  H 
Sbjct:  285 PITPLLTQLTYAGILDDLYEF-NSGIKIKE---KDMNFNYKEDKIWNDLKFLNFGSIGPQLNKLAKELQTQYDTRHKAESVHEIKEFVDSLGSLQQRQAFLKNHT 385          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Nematostella
Match: EDO39542 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9U5])

HSP 1 Score: 436.032 bits (1120), Expect = 5.450e-147
Identity = 240/554 (43.32%), Postives = 372/554 (67.15%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSP-RENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQ-NXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYG--SIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGS 1712
            MN+  A+K Y+  MI+    GMKVL++D+ET  +V+ V+S +E+++ +VYL ER+ +P RE + +L  IC+LRPT  N++ L  EL++P Y  Y I+FS+ + K  ++ LAEAD  E+V+ +QE +AD++   P +F LN    +   +W+I SL R+ +G+ +LLL+LK  P IRYQ SSEVA RLAE++R  I+ EA LF+ ++ +   LLLI+DRR DPVTPLLNQWTYQAM++ELL + NN V++ +      ++ E+ +S +HDEFY +N+Y NFGEI  NIK  M++ Q    +  K++SI DMK F+ENYP F+KMS  VS+HV ++SE+S++++   LL VSE EQ +      ++H++ +Q I+ L+ N ++ + D +R+V++YAL+Y+RH   + S L+N+L ++ GV E   +++  +++Y   S++  +LF +    L +   + K +KGVENIYTQH PL+S   + + + +L +  FPY+ G +    +  D+I+F+VGG T+EE+ A++NIN+   G  VILGGT I N  SFL EV S
Sbjct:    1 MNVILAVKQYVTKMIEESGAGMKVLLMDKETTGIVSMVYSQTEVLQKEVYLFERVDTPGRETMKHLKAICFLRPTPENIDHLCSELKSPKYGVYYIYFSNFVPKASIRALAEADDQEVVREVQEYYADYFAISPHVFSLNSPASMKGGQWDIDSLDRSCEGVLALLLSLKKCPMIRYQQSSEVAHRLAERIRQKINGEAKLFDFRRPDVPPLLLILDRRDDPVTPLLNQWTYQAMVHELLTIRNNRVDLSKCPDVARDLQEVVMSAEHDEFYQKNMYLNFGEIGQNIKTLMDDFQQHVKSNQKLESISDMKNFVENYPQFKKMSGTVSKHVTMVSELSRLVSDRCLLDVSEIEQELACQ---NDHSAALQNIRRLMANDKVSELDLLRVVMIYALRYERHTNNDVSTLVNMLARR-GVGEQYKRLVPAIVQYAGRSVRGSDLFGQNKTPLSLTRKILKGLKGVENIYTQHAPLLSETIDGLIKGRLKDAQFPYM-GHAQLRDRPQDIIVFIVGGATYEEAQAVYNINKTQPGIKVILGGTTIHNCRSFLEEVSS 549          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Nematostella
Match: EDO43369 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYS2])

HSP 1 Score: 67.781 bits (164), Expect = 5.515e-12
Identity = 117/550 (21.27%), Postives = 235/550 (42.73%), Query Frame = 3
Query:  144 KVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRN-----PHYKSYDIHFSHSLSKQYLKLLAEADHFE--LVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLS----RTIDGITSLLLALKIFPQIRYQ--NSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDN--HNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKI---NSLGIPSNLFKSIKG-------------VENIY--TQHQPLISNIANQVNEQKLNEVLFPYI------------SGCSPSNHK----------YTDLIIFVVGGVTFEESLAIHNI 1628
            KVL++D  ++++V+S   M ++++  + + E +   RE L  L  I  + P   +  +LE+ + +       YKS  I F+       +  L +    +  +VK ++E    F PC+  +F L+  +   +R +  +  S    +  + + +L  +L  FP IRY+  N   V      K R+ + +  +    +    + L+++DR  DP++PLL++ TYQAM  +L+ + N+    +      +    ++DE + +  + +  ++   I   ++E   K    +  + ++D++  ++  P ++    K   H   ++E           ++   EQ +      D+      V+ I  +L + +I+ HD ++++LLY                 +L K  G+SE  +  +      G  + P   + +I     LGIP      IKG             +E  Y  ++  P I +I     E  L+E LFP++            +  S +N +             LI+F+VGGVT+ E+ + + +
Sbjct:   21 KVLVVDHLSMRMVSSCLKMHDLLEVGITIVENVHIQREPLNRLEAIYLITP---DEKVLEEVIEDFDSFFLTYKSAHIFFTEVCPDHLMTKLHQIRKCKVIIVKTLKEIDIAFLPCEEQVFSLDNPSTF-DRFFSPAPTSLLPQKIAEQLATLCASLGEFPSIRYKPDNPKMVGFANQVKARLDVYKRDDPSIGEGRYPAQLILLDRGFDPISPLLHELTYQAMAYDLINIKNDVFKYN----FNLFFLLENDEMWVKLRHLHIADVSRKIPEEIKEFSEKEKLPSSQNKMKDLQAMLKKAPQYKDQVKKFMVHF-SLAEECMRHYNEVANKLCPVEQELATGFDKDDVKLKEPVKGIVPVLLDHDIRLHDKLKVILLY-----------------ILFKHEGISEENLDKL-----CGHAQIPVEDRAEIMNMEYLGIP-----VIKGSIQKKYSPVRKTRMETFYLLSRWVPYIKDIIEDAIEGTLSEQLFPFLVKRALPESSSLRTCLSDTNWRKRVTAAPVSLVPRLIVFIVGGVTYSETRSAYEV 534          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Nematostella
Match: EDO43368 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYS1])

HSP 1 Score: 62.7734 bits (151), Expect = 2.031e-10
Identity = 121/566 (21.38%), Postives = 240/566 (42.40%), Query Frame = 3
Query:  150 LMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRN-P-HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLN--------LKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSS----EVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNC--------------VNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQM----IKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYIS----GCSPS----------------NHKYTD------LIIFVVGGVTFEESLAIHNINQQISGKNVILG 1664
            L+LD  + ++++S   M +++   V + E +   RE L  L  I  L P   +++LL  + +  P  YK   + F+       L  L     F  +K ++E    F P +  +F L+            + N+E  I  L R  + + +L   L  +P IR+++ S    E A  +  +L  + + +  + E S     S L+I+DR  DPV+PLL++ T QAM  +LL++ N+               + I  G +S+ +  P   +  H ++ +   +I   IK F  + +  ++ ++   +++D+++ ++  P ++K   +   H   ++E      +    ++   EQ++     +D     ++     I  LL +  +  +D IR+++LY L         T   +  L + + + + +  II  +         NL    +   G      +  +  E  Y  ++  P + +I     E KL+   FP++S    G S S                +   TD      LI+F++GGV++ E+ A + +    +   V++G
Sbjct:    1 LVLDHLSTRMISSCVKMHDLIDNGVTVVENLEKRREPLPQLEAIYILTPEEDSISLLVADFKEYPLKYKGAHLFFTEVCPDSLLVQLQGIKRF--IKTLKEINIAFLPYESQVFSLDSTQGFGKFYAPGVENKE-RIQYLERMAEQLATLCATLGEYPSIRFRHESPKLTEFAHIVQGRLDAYKADDPTMGEGSAHKHRSQLIILDRAFDPVSPLLHELTLQAMAYDLLDITNDVYKYGCSARVTVPILIFIFSG-VSDADNDPMWVKLRHLHIADVSRKISDEIKEFAGKKRMSTTEKS---TLKDLQVMLKKMPQYQKELGQYILHF-HLAEDCMNHYQETADKLCGVEQDLATG--VDKTGESIRDPMKNIVPLLLDKNVNIYDKIRIIILYIL----FKNGITEENLTKLCQHAQIPQSDRTIITNMA--------NLGIPIVQDSGKKKPKPERKERDETFYQLSRWVPYVKDIMEDAIEDKLSSKAFPFLSQRAAGGSASVCFLFPSARYGNWYKHDKACTDSRSLPRLIVFIMGGVSYSETRAAYQVTAANANWEVLIG 544          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Medaka
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:101171176])

HSP 1 Score: 438.343 bits (1126), Expect = 2.003e-147
Identity = 250/565 (44.25%), Postives = 375/565 (66.37%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITS-PRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNK-SSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDK 1742
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  R+++ +L  IC+LRPT  NV  L +ELR P Y  Y I+FS+ +SK  +K+LAEAD  E+V  +QE + DF   +P LF LNL+ V   R WE S LSR   G+TS+LLALK  P IRYQ SS++++RLAE ++  I++E  LF+  K     LLLI+DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  NLY NFGEI +NIK  ME+ Q K    Q K++SI DMK F++NYP F+KMS  VS+HV ++ E+S+++++ KL+ VSE EQ +      ++H++  Q+++ LL N  I + DA+RLV+LYAL+Y+RH      +LM   L + GVSE   ++++ ++EYG   I+  +L     +++ I    FK +KGVEN+YTQHQPL+ +  +Q+ + +L +  FPY+ G S    +  D+++F++GG T+EE+L ++N+N+   G  ++LGG+ I N  SFL EV    G+S   D+
Sbjct:    1 MNVTLAVKQYISKMIESSGPGMKVLLMDKETTSIVSVVYTQSEILQKEVYLFERIDSQSRDSMKHLKAICFLRPTKENVQHLIQELRRPKYSIYFIYFSNVISKSEIKVLAEADEQEVVAEVQEFYGDFIAVNPHLFSLNLQGVARGRSWEPSMLSRCTQGLTSVLLALKKCPMIRYQLSSDMSKRLAESVKQIITKEYELFDFRKTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGLNNNRIDLSRVPGISKDLKEVVLSAENDEFYANNLYLNFGEIGTNIKNLMEDFQKKRPKGQEKLESISDMKAFVDNYPQFKKMSGTVSKHVTVVGELSRLVSERKLMEVSEVEQELACQ---NDHSNAQQVVRRLLQNPRISELDAVRLVMLYALRYERHSSSILPSLME-DLNRRGVSERHRRMVQAVVEYGGKRIRGSDLIT-PTDAVSITKQFFKGLKGVENVYTQHQPLLHDTLDQLIKGRLKDSQFPYL-GASSLRDRPQDIMVFIIGGATYEEALTVYNLNRNTPGVRIVLGGSTIQNTKSFLEEVMLATGQSSGSDR 559          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Medaka
Match: vps45 (vacuolar protein sorting 45 homolog [Source:NCBI gene;Acc:101171176])

HSP 1 Score: 405.986 bits (1042), Expect = 4.138e-135
Identity = 240/565 (42.48%), Postives = 362/565 (64.07%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITS-PRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFE-SKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHE-----GEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKS-SNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGS--IKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDK 1742
            MN+  A+K YI  MI++   GMKVL++D+ET  +V+ V++ SEI++ +VYL ERI S  R+++ +L  IC+LRPT  NV  L +ELR P Y  Y I+FS+ +SK  +K+LAEAD  E+V  +Q                        R WE S LSR   G+TS+LLALK  P IRYQ SS++++RLAE ++  I++E  LF+  K     LLLI+DR  D +TPLLNQWTYQAM++ELL ++NN +++        ++ E+ LS ++DEFY  NLY NFGEI +NIK  ME+ Q K    Q K++SI DMK F++NYP F+KMS  VS+HV ++ E+S+++++ KL+ VSE EQ +      ++H++  Q+++ LL N  I + DA+RLV+LYAL+Y+RH      +LM   L + GVSE   ++++ ++EYG   I+  +L     +++ I    FK +KGVEN+YTQHQPL+ +  +Q+ + +L +  FPY+ G S    +  D+++F++GG T+EE+L ++N+N+   G  ++LGG+ I N  SFL EV    G+S   D+
Sbjct:    1 MNVTLAVKQYISKMIESSGPGMKVLLMDKETTSIVSVVYTQSEILQKEVYLFERIDSQSRDSMKHLKAICFLRPTKENVQHLIQELRRPKYSIYFIYFSNVISKSEIKVLAEADEQEVVAEVQ-----------------------GRSWEPSMLSRCTQGLTSVLLALKKCPMIRYQLSSDMSKRLAESVKQIITKEYELFDFRKTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGLNNNRIDLSRVPGISKDLKEVVLSAENDEFYANNLYLNFGEIGTNIKNLMEDFQKKRPKGQEKLESISDMKAFVDNYPQFKKMSGTVSKHVTVVGELSRLVSERKLMEVSEVEQELACQ---NDHSNAQQVVRRLLQNPRISELDAVRLVMLYALRYERHSSSILPSLME-DLNRRGVSERHRRMVQAVVEYGGKRIRGSDLIT-PTDAVSITKQFFKGLKGVENVYTQHQPLLHDTLDQLIKGRLKDSQFPYL-GASSLRDRPQDIMVFIIGGATYEEALTVYNLNRNTPGVRIVLGGSTIQNTKSFLEEVMLATGQSSGSDR 536          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 118.627 bits (296), Expect = 9.178e-28
Identity = 136/599 (22.70%), Postives = 269/599 (44.91%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMI--KNDDNG-MKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELR---NPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHN--SCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYG----SIKSPNLFKEKINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYIS-----------GCSPSNHKYTD---------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            + LK  +   I+N +  K  + G  KVL++D+ ++K+V+S   M++IM   + + E IT  RE L  +  I  + P+  +V  L ++ R   NP Y++  + F+ ++      LL ++   + +K + E    F P +  +F L+              + + + L R  + I ++   LK +P +RY    ++ + +A+ L EKL  + + +  + E      + LLI+DR  DPV+PLL++ T QAM  +LL ++N+        + E  + E+ L  D D+ +    + +  E+ + +   ++E   +K  N  +  +++++   ++  P ++K  +K S H+ +  +           ++   EQ++      +       +++I  +L ++ +   D IR++LLY    +      T   +  LL+ + +   +  II  +   G    S  SP+  KE+++               E  Y  ++  PLI ++     E KL+   FPYIS              PS  +Y +               +I+F++GGVT+ E   ++ + Q       ++G T IL    +L E+
Sbjct:    4 LGLKAIVGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTDTIPDSLFGLLTKSRASKAMKALTEIHIAFLPYESQVFSLDKAEAFQDFYSPFKADVKSNMLERCAEQIATMCATLKEYPGVRYRGDYKDCAVLAQMLQEKLDGYKADDPTMGEGPDKCRTQLLIVDRGFDPVSPLLHELTLQAMAYDLLGIENDVYSFETSGMGETRMKEVVLDED-DDLWLSLRHKHIAEVSTAVTRSLKEFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHLHLAEDCMNRYQGTVD-KLCRVEQDLAMGTDAEGEKIKDPMRLIVPVLLDANVSVFDKIRIILLYIFLKN----GVTEENLCKLLQHASIPPEDSDIISNMAHMGVPIISEVSPDR-KERVS---------------EQTYQLSRWTPLIKDLIEDAIEDKLDPKQFPYISQRQVSAKAIASASDPSIARYGNWHKNRGPTEMKTGPRIIVFIIGGVTYSEMRCVYEVTQANGKWEALVGSTHILTPTKYLKEL 580          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 115.546 bits (288), Expect = 1.037e-26
Identity = 136/602 (22.59%), Postives = 274/602 (45.51%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMI--KNDDNG-MKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELR---NPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNL-FKEKINSLGIP----SNLFKSIKG------VENIY--TQHQPLISNIANQVNEQKLNEVLFPYIS----------------GCSPSNHKYTDL------IIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            + LK  +   I+N +  K  + G  KVL++D+ ++K+V+S   M++IM   + + E IT  RE L  +  I  + P+  +V  L ++ R   NP Y++  + F+ ++      LL ++   + +K + E    F P +  +F L+              + + + L R  + I ++   LK +P +RY    ++ + +A+ L EKL  + + +  + E      + LLI+DR  DPV+PLL++ T QAM  +LL ++N+        + E  + E+ L  D D+ +    + +  E+ + +   ++E   +K  N  +  +++++   ++  P ++K  +K S H+  ++E      +  + ++   EQ++      +       +++I  +L ++ +   D IR++LLY                  +  ++GV+E   + +  L+++ SI   +      +  +G+P      + K+ K        E  Y  ++  PLI ++     E KL+   FPYIS                G    N   T++      I+F++GGVT+ E   ++ + Q       ++G T IL    +L E+
Sbjct:    4 LGLKAIVGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTDTIPDSLFGLLTKSRASKAMKALTEIHIAFLPYESQVFSLDKAEAFQDFYSPFKADVKSNMLERCAEQIATMCATLKEYPGVRYRGDYKDCAVLAQMLQEKLDGYKADDPTMGEGPDKCRTQLLIVDRGFDPVSPLLHELTLQAMAYDLLGIENDVYSFETSGMGETRMKEVVLDED-DDLWLSLRHKHIAEVSTAVTRSLKEFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHL-HLAEDCMNRYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRLIVPVLLDANVSVFDKIRIILLY------------------IFLKNGVTE---ENLCKLLQHASIPPEDSDIISNMAHMGVPIISEGTVKKAKKADRKERVSEQTYQLSRWTPLIKDLIEDAIEDKLDPKQFPYISQRQVSAKASAPSSARYGNWHKNRGPTEMKTGPRIIVFIIGGVTYSEMRCVYEVTQANGKWEALVGSTHILTPTKYLKEL 582          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Medaka
Match: stxbp1b (syntaxin-binding protein 1 [Source:NCBI gene;Acc:101159753])

HSP 1 Score: 112.849 bits (281), Expect = 7.373e-26
Identity = 136/590 (23.05%), Postives = 269/590 (45.59%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMI--KNDDNG-MKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELR---NPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVI------INREWEISSLSRTIDGITSLLLALKIFPQIRY----QNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNN-----CVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQ-NKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNH--NSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYG----SIKSPNLFKEKINSLGIPSNLFKSIKGVENIY--TQHQPLISNIANQVNEQKLNEVLFPYI-----------SGCSPSNHKYTD---------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQIL 1679
            + LK  +   I+N +  K  + G  KVL++D+ ++K+V+S   M++IM   + + E IT  RE L  +  I  + P+  +V  L ++ R   NP Y++  + F+ ++      LL ++   + +K + E    F P +  +F L+              + + + L R  + I ++   LK +P +RY    ++ + +A+ L EKL  + + +  + E      + LLI+DR  DPV+PLL++ T QAM  +LL ++N+        + E  + E+ L  D D+ +    + +  E+ + +   ++E   +K  N  +  +++++   ++  P ++K  +K S H+  ++E      +  + ++   EQ++      +       +++I  +L ++ +   D IR++LLY    +      T   +  LL+ + +   +  II  +   G    S  SP+  KE+++               E  Y  ++  PLI ++     E KL+   FPYI           S   PS  +Y +               +I+F++GGVT+ E   ++ + Q  +GK   L G + L
Sbjct:    4 LGLKAIVGEKIMNNVIKKAREKGKWKVLVVDKLSMKMVSSCCKMTDIMSEGITIVEDITKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQNPRYRAAHVFFTDTIPDSLFGLLTKSRASKAMKALTEIHIAFLPYESQVFSLDKAEAFQDFYSPFKADVKSNMLERCAEQIATMCATLKEYPGVRYRGDYKDCAVLAQMLQEKLDGYKADDPTMGEGPDKCRTQLLIVDRGFDPVSPLLHELTLQAMAYDLLGIENDVYSFETSGMGETRMKEVVLDED-DDLWLSLRHKHIAEVSTAVTRSLKEFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHL-HLAEDCMNRYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRLIVPVLLDANVSVFDKIRIILLYIFLKN----GVTEENLCKLLQHASIPPEDSDIISNMAHMGVPIISEVSPDR-KERVS---------------EQTYQLSRWTPLIKDLIEDAIEDKLDPKQFPYISQRQVSAKAIASASDPSIARYGNWHKNRGPTEMKTGPRIIVFIIGGVTYSEMRCVYEVTQA-NGKWEALVGDRTL 570          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Planmine SMEST
Match: SMESG000046309.1 (SMESG000046309.1)

HSP 1 Score: 1041.57 bits (2692), Expect = 0.000e+0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 3
Query:   78 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 1706
            MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV
Sbjct:    1 MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEV 543          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Planmine SMEST
Match: SMESG000046314.1 (SMESG000046314.1)

HSP 1 Score: 1040.8 bits (2690), Expect = 0.000e+0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 3
Query:  117 MIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDKF 1745
            MIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDKF
Sbjct:    1 MIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDKF 543          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Planmine SMEST
Match: SMESG000028362.1 (SMESG000028362.1)

HSP 1 Score: 985.712 bits (2547), Expect = 0.000e+0
Identity = 515/516 (99.81%), Postives = 516/516 (100.00%), Query Frame = 3
Query:  198 MSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDKF 1745
            MSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSS+QAKIDSIEDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDKF
Sbjct:    1 MSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWEISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANLFESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSSQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIVNHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGESITIDKF 516          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Planmine SMEST
Match: SMESG000013817.1 (SMESG000013817.1)

HSP 1 Score: 100.138 bits (248), Expect = 8.124e-22
Identity = 133/593 (22.43%), Postives = 260/593 (43.84%), Query Frame = 3
Query:   84 LKEAIKSYI----INMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNP--HYKSYDIHFSHSLSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVII------NREWEISSLSRTIDGITSLLLALKIFPQIRYQ----NSSEVARRLAEKLRIHISREANLFESKQNXXXXXXXXXXXXXPVTPLLNQWTYQAMINELLEVDNNC---VNIHEGEISEINLSPDH-DEFY----HQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVSQHVXXXXXXXXXXXXXXXXRVSECEQNIV-----NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSNLMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKSIKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTD--------------------------LIIFVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFL 1697
            LKE++   I    I  IK +    KVL++D+ + ++++S   M +IM   + L E I   RE +L L  I  + PT +++  L  +       YK+  + F+ +   +    L ++   + +K ++E    F P +  +F L+             +      L R  + I +L   LK +P IRY+     ++E A+ + +KL  + + + ++ E      S L+I+DR  DP++PLL++ T+QAM  +L+ ++N+    +NI   E  E  +  D  DE +    H ++     ++ S +K F +E + + +      ++ D+   ++  P ++K  +  S H  +  +       +   ++ + EQ++        + I +H   ++ I  +L +  +  +D +R++LLY +    H        ++ L++   + E +  II+ L   G   S    ++K     + SN  +     +   ++  P + +I     E KL+   FP I G  P     T                           LI FVVGG+++ E  + + +  Q     V++GGT +L   +FL
Sbjct:    2 LKESVAKKILDDVIRTIKKEGE-WKVLIVDKLSTRMISSCCKMHDIMNEGITLVEDINKKRE-ILPLEAIYLITPTEKSIKSLMADFTGSKNQYKAAHVFFTEACPDESFNELCKSPCAKYIKTLREINIAFLPYESRVFSLDSPETTQFYFNPNAKTGRTLHLERIAEQIATLCATLKEYPPIRYRAQWDKNAEFAQLVQQKLDAYKADDPSMGEGPFKDRSQLVILDRGFDPISPLLHELTFQAMSYDLIAIENDVYRYLNISGPEEREKEVVLDENDELWVELRHLHIAVVSNQVTSRLKKFADEKRMRGTGDKT--TMRDLSQMVKKMPQYQKELSMYSTHFHLAEDCMHAYQGHVD-KLCKVEQDLAMGTDAEGEKIKDH---MRQIVPILLDQSVTAYDKLRIILLYII----HRGGIAEENIDKLVQHGQIPEPQTAIIKNLTMLGV--SSTAGRKKFTQPYLVSNRKEREGEQKYQMSRWTPYMKDIMEDACEDKLDVKQFP-IFGGGPVRANMTGRGGGQLSARYGQWHKDKAQQQRNGPRLIFFVVGGISYSEMRSAYEVANQYKNCEVMVGGTHLLTPEAFL 579          
BLAST of Vacuolar protein sorting-associated protein 45 vs. Planmine SMEST
Match: SMESG000044570.1 (SMESG000044570.1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.376e-15
Identity = 88/347 (25.36%), Postives = 163/347 (46.97%), Query Frame = 3
Query:  111 INMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVYLCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHSLSKQYLKLLA----EADHFELVKNIQESFADFYPCDPFLFHLNLK----------NVIINREWEISSLSRTI-DGITSLLLALKIFPQIRY--QNSSE-VARRLAEKLRIHISREAN-LF-----ESKQNXXXXXXXXXXXXXPV-----------TPLLNQWTYQAMINELLEVDNNCVNI--HEGEISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSIEDMKMFIENYPSFRKMSAKVS 1040
            I  + N D   KVL+ D+  L +++++F+   +    V +   I S RE +  +  I ++ PT  N+N L K++ N  Y+SY I+F   ++++ L+ LA    ++D    +  I + + +F   +  LF L+            N     + EI ++  +I DG+ S+ + +   P IR    N++E VA+RL +KL+ ++    N LF      +  + SS          P+           TP+ + WTYQA+++++L +  N VNI     + +   L  ++DEF+    +    EI   I+  +E +++K    A++ S   +    +   S  K+S  VS
Sbjct:   23 ITKVVNYDKLWKVLICDKHGLDIISTLFTQKSLRDLGVTISLNINSEREPIPDVPAIYFVLPTSENINKLCKDIDNGIYESYYINFISPINREKLEQLAYSTFQSDSVNQISKIFDQYTNFICLEDDLFVLHDDKTGSMSYSSLNGAGKSDQEIDAIINSIVDGLFSVCVTIGTIPVIRCPPGNAAEMVAKRLDKKLKDNLRDSRNSLFSGLGMSTLSDHSSGTQSHLSFERPLLVILDRSLDLATPINHPWTYQALVHDILNIKFNTVNIPSQTKKTNSYELWCENDEFWRDERHKPLPEIADVIQQGLEVIKSKEKEMAQLKSSMGLGGDSDITDSTNKLSDTVS 369          
The following BLAST results are available for this feature:
BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
VPS456.319e-13944.12vacuolar protein sorting 45 homolog [Source:HGNC S... [more]
VPS452.691e-13443.85vacuolar protein sorting 45 homolog [Source:HGNC S... [more]
VPS452.268e-12643.99vacuolar protein sorting 45 homolog [Source:HGNC S... [more]
VPS456.100e-10541.53vacuolar protein sorting 45 homolog [Source:HGNC S... [more]
VPS452.770e-9743.57vacuolar protein sorting 45 homolog [Source:HGNC S... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps-456.286e-10437.86Related to yeast Vacuolar Protein Sorting factor ... [more]
T07A9.106.368e-2323.16pep chromosome:WBcel235:IV:390512:393158:1 gene:WB... [more]
unc-185.710e-2222.73Putative acetylcholine regulator unc-18 [Source:U... [more]
vps-451.371e-1934.96Related to yeast Vacuolar Protein Sorting factor ... [more]
sly-14.439e-1122.71SLY1 homolog [Source:UniProtKB/TrEMBL;Acc:Q20364][more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Vps455.103e-12841.07gene:FBgn0261049 transcript:FBtr0082070[more]
Rop1.662e-1921.66gene:FBgn0004574 transcript:FBtr0310632[more]
Rop1.662e-1921.66gene:FBgn0004574 transcript:FBtr0073263[more]
Slh2.269e-1021.53gene:FBgn0264978 transcript:FBtr0335169[more]
Slh2.269e-1021.53gene:FBgn0264978 transcript:FBtr0077782[more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps453.295e-14144.09vacuolar protein sorting 45 homolog [Source:ZFIN;A... [more]
stxbp1b8.385e-2622.03syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-G... [more]
stxbp1b8.385e-2622.03syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-G... [more]
stxbp1b1.857e-2521.81syntaxin binding protein 1b [Source:ZFIN;Acc:ZDB-G... [more]
stxbp26.352e-2522.64syntaxin binding protein 2 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps458.939e-14043.96vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
vps456.116e-13743.61vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
vps451.715e-12742.57vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
vps451.664e-12640.26vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
vps453.732e-11641.10vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Vps456.163e-14243.94vacuolar protein sorting 45 [Source:MGI Symbol;Acc... [more]
Stxbp21.221e-2724.23syntaxin binding protein 2 [Source:MGI Symbol;Acc:... [more]
Stxbp22.211e-2724.27syntaxin binding protein 2 [Source:MGI Symbol;Acc:... [more]
Stxbp12.524e-2422.01syntaxin binding protein 1 [Source:MGI Symbol;Acc:... [more]
Stxbp14.036e-2321.95syntaxin binding protein 1 [Source:MGI Symbol;Acc:... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O08700|VPS45_RAT9.810e-14244.30Vacuolar protein sorting-associated protein 45 OS=... [more]
sp|P97390|VPS45_MOUSE4.311e-14143.94Vacuolar protein sorting-associated protein 45 OS=... [more]
sp|Q9NRW7|VPS45_HUMAN3.034e-13844.12Vacuolar protein sorting-associated protein 45 OS=... [more]
sp|Q54GE3|VPS45_DICDI5.691e-11238.64Vacuolar protein sorting-associated protein 45 OS=... [more]
sp|O49048|VPS45_ARATH8.204e-9736.61Vacuolar protein sorting-associated protein 45 hom... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QI609.441e-15947.04Vacuolar protein sorting-associated protein 45 OS=... [more]
R7TQD91.306e-15346.62Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A1S3GYE51.695e-15344.38vacuolar protein sorting-associated protein 45 OS=... [more]
X1YZB12.246e-15346.62Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A433T7Z42.790e-15346.76Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps454.719e-14444.09pleckstrin homology domain-containing family O mem... [more]
vps455.741e-12742.02pleckstrin homology domain-containing family O mem... [more]
vps453.263e-12640.39pleckstrin homology domain-containing family O mem... [more]
stxbp23.697e-3123.63syntaxin binding protein 2 [Source:NCBI gene;Acc:1... [more]
stxbp31.425e-2524.83syntaxin binding protein 3 [Source:NCBI gene;Acc:1... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
vps451.232e-14342.93vacuolar protein sorting 45 homolog [Source:ZFIN;A... [more]
stxbp1a1.103e-2022.20syntaxin binding protein 1a [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
VPS458.395e-7632.01Protein of the Sec1p/Munc-18 family; essential for... [more]
SLY18.855e-1323.13Hydrophilic protein involved in ER/Golgi vesicle t... [more]
VPS331.539e-727.62ATP-binding protein that is a subunit of the HOPS ... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO395425.450e-14743.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO433695.515e-1221.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO433682.031e-1021.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps452.003e-14744.25vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
vps454.138e-13542.48vacuolar protein sorting 45 homolog [Source:NCBI g... [more]
stxbp1b9.178e-2822.70syntaxin-binding protein 1 [Source:NCBI gene;Acc:1... [more]
stxbp1b1.037e-2622.59syntaxin-binding protein 1 [Source:NCBI gene;Acc:1... [more]
stxbp1b7.373e-2623.05syntaxin-binding protein 1 [Source:NCBI gene;Acc:1... [more]
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BLAST of Vacuolar protein sorting-associated protein 45 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000046309.10.000e+0100.00SMESG000046309.1[more]
SMESG000046314.10.000e+0100.00SMESG000046314.1[more]
SMESG000028362.10.000e+099.81SMESG000028362.1[more]
SMESG000013817.18.124e-2222.43SMESG000013817.1[more]
SMESG000044570.12.376e-1525.36SMESG000044570.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015161 ID=SMED30015161|Name=Vacuolar protein sorting-associated protein 45|organism=Schmidtea mediterranea sexual|type=transcript|length=1793bp
ATACTATTTATCACTTTATTTATCAGAATTTTTAATTATTAAAATTTTGA
TTCCACATAATATTAGTATTCTATTTGATGAACTTAAAGGAGGCAATAAA
ATCTTATATAATTAATATGATTAAAAATGATGATAATGGAATGAAAGTTT
TAATGTTAGACGAAGAAACCTTAAAAGTGGTCAACTCTGTTTTTTCAATG
TCAGAAATAATGAAATTTGATGTTTATTTATGTGAAAGAATTACTTCACC
TAGAGAAAATTTACTGTATTTAAATTGTATTTGTTATCTAAGGCCAACAG
TTCGAAATGTAAATCTTTTAGAAAAAGAATTAAGAAATCCTCACTACAAG
TCATATGATATTCATTTTTCTCATAGTCTTTCCAAGCAGTATTTAAAATT
ATTGGCTGAAGCTGATCATTTTGAACTTGTTAAAAATATTCAAGAATCAT
TCGCTGACTTCTATCCATGTGATCCATTTTTATTTCATTTAAATTTAAAA
AATGTTATTATTAATCGTGAATGGGAAATTAGTTCACTTAGTCGAACTAT
TGATGGAATAACTTCTTTATTATTAGCACTCAAAATTTTCCCACAAATCC
GATATCAGAATTCATCAGAAGTTGCAAGGAGATTGGCGGAAAAATTGCGA
ATTCACATATCAAGAGAAGCAAATTTATTTGAAAGTAAACAAAATCGGTC
ATCTTTACTTTTAATAATCGATAGACGGCTGGATCCAGTTACTCCATTAT
TGAATCAATGGACTTATCAAGCCATGATTAATGAACTGCTTGAAGTTGAC
AATAATTGTGTCAACATTCATGAAGGTGAAATTAGTGAAATTAATTTAAG
CCCAGATCACGATGAATTTTATCATCAAAATCTTTATAATAATTTTGGTG
AAATATGTTCTAATATAAAGTGTTTTATGGAAGAATTACAGAATAAATCC
TCTAACCAAGCTAAAATAGATTCGATTGAAGATATGAAAATGTTTATAGA
AAATTATCCTAGTTTTCGTAAAATGTCTGCTAAAGTATCCCAACACGTTT
TAATAATATCAGAAATATCCAAAATAATTAATAAAAATAAATTACTACGA
GTTAGTGAATGTGAGCAAAATATTGTTAATCATCAAATGATTGATAATCA
CAATTCATGCGTTCAAATGATAAAAGACTTGTTGGGTAATTCCGAAATAA
AAAAACACGATGCTATTAGATTAGTTTTATTGTACGCTCTTAAATATGAT
CGTCACCCAAGAAAAGAAACTTCGAATTTAATGAATTTATTATTAAAACA
AAGTGGTGTTTCAGAAATGGAAATTCAAATAATTCGTATACTAATGGAGT
ACGGATCAATAAAATCGCCAAATCTTTTCAAAGAAAAAATAAATTCCCTG
GGTATACCTAGTAATTTATTTAAAAGTATTAAAGGAGTTGAAAATATTTA
TACCCAACATCAGCCTCTTATTAGTAATATTGCTAATCAAGTAAATGAGC
AAAAATTGAATGAAGTTTTATTTCCATATATTAGTGGATGTAGCCCTAGT
AATCACAAGTATACTGATTTAATAATTTTCGTTGTAGGAGGTGTTACTTT
TGAAGAATCTTTAGCTATTCATAATATTAATCAACAAATTTCTGGAAAAA
ATGTGATATTAGGTGGCACACAAATTTTAAATTATCATTCGTTTCTTTTA
GAAGTGGGTTCTTGGATAGGCGAATCGATAACCATTGATAAATTTTAGTT
AATTTAACAGTAATTGCGGATTTTTGATCATGTTTACTCTATT
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protein sequence of SMED30015161-orf-1

>SMED30015161-orf-1 ID=SMED30015161-orf-1|Name=SMED30015161-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=557bp
MNLKEAIKSYIINMIKNDDNGMKVLMLDEETLKVVNSVFSMSEIMKFDVY
LCERITSPRENLLYLNCICYLRPTVRNVNLLEKELRNPHYKSYDIHFSHS
LSKQYLKLLAEADHFELVKNIQESFADFYPCDPFLFHLNLKNVIINREWE
ISSLSRTIDGITSLLLALKIFPQIRYQNSSEVARRLAEKLRIHISREANL
FESKQNRSSLLLIIDRRLDPVTPLLNQWTYQAMINELLEVDNNCVNIHEG
EISEINLSPDHDEFYHQNLYNNFGEICSNIKCFMEELQNKSSNQAKIDSI
EDMKMFIENYPSFRKMSAKVSQHVLIISEISKIINKNKLLRVSECEQNIV
NHQMIDNHNSCVQMIKDLLGNSEIKKHDAIRLVLLYALKYDRHPRKETSN
LMNLLLKQSGVSEMEIQIIRILMEYGSIKSPNLFKEKINSLGIPSNLFKS
IKGVENIYTQHQPLISNIANQVNEQKLNEVLFPYISGCSPSNHKYTDLII
FVVGGVTFEESLAIHNINQQISGKNVILGGTQILNYHSFLLEVGSWIGES
ITIDKF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR043154Sec-1-like_dom1
IPR043127Sec-1-like_dom3a
IPR036045Sec1-like_sf
IPR001619Sec1-like
IPR027482Sec-1-like_dom2
Vocabulary: biological process
TermDefinition
GO:0016192vesicle-mediated transport
GO:0006904vesicle docking involved in exocytosis
GO:0006810transport
GO:0006886intracellular protein transport
GO:0007596blood coagulation
GO:0015031protein transport
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
GO:0005768endosome
GO:0005794Golgi apparatus
GO:0010008endosome membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0000139Golgi membrane
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043154Sec1-like, domain 1GENE3DG3DSA:3.40.50.2060coord: 1..125
e-value: 1.4E-27
score: 97.7
IPR001619Sec1-like proteinPFAMPF00995Sec1coord: 23..540
e-value: 2.1E-111
score: 373.9
IPR001619Sec1-like proteinPIRSFPIRSF005715VPS45_Sec1coord: 1..549
e-value: 3.7E-135
score: 449.4
IPR001619Sec1-like proteinPANTHERPTHR11679VESICLE PROTEIN SORTING-ASSOCIATEDcoord: 1..542
NoneNo IPR availableGENE3DG3DSA:1.25.40.60coord: 337..462
e-value: 7.9E-101
score: 339.8
NoneNo IPR availablePANTHERPTHR11679:SF3VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 45coord: 1..542
IPR043127Sec1-like, domain 3aGENE3DG3DSA:3.90.830.10coord: 227..336
e-value: 7.9E-101
score: 339.8
IPR027482Sec1-like, domain 2GENE3DG3DSA:3.40.50.1910coord: 145..542
e-value: 7.9E-101
score: 339.8
IPR036045Sec1-like superfamilySUPERFAMILYSSF56815Sec1/munc18-like (SM) proteinscoord: 1..543