SMED30015160

Overview
NameSMED30015160
Smed IDSMED30015160
Length (bp)2544
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015160 (SMED30015160) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015160 (SMED30015160) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015160 (SMED30015160) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015160 (SMED30015160) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015160

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30015160h1SMcG0013497 Contig49820uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015160h1SMcG0013497 Contig49820newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015160h1SMcG0013498 Contig49820uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015160h1SMcG0013498 Contig49820newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30015160h1SMcG0013497 dd_Smed_v4_2618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015160h1SMcG0013497 dd_Smed_v4_2618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015160h1SMcG0013497 dd_Smed_v4_2618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30015160h1SMcG0013497 dd_Smed_v4_2618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30015160h1SMcG0013497 dd_Smed_v4_2618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30015160h1SMcG0013497 dd_Smed_v4_2618_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015160 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002190:47181..54521 +12119
Homology
[Close]
BLAST of SMED30015160 vs. TrEMBL
Match: R7V6U0 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_226983 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 5.906e-8
Identity = 33/61 (54.10%), Postives = 46/61 (75.41%), Query Frame = 2 
Query: 1742 NSDLESGIVE-GYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLK 1921
             SD+E   V+ GY SD D L+++     NSGYMSEGG+ LYA++MQQRFREG++AV+E ++
Sbjct:  710 GSDVEGFDVQAGYMSDGDILRSNQSDDMNSGYMSEGGVSLYAKRMQQRFREGMMAVKECMQ 770
[Close]
BLAST of SMED30015160 vs. TrEMBL
Match: A0A5B7EY73 (Uncharacterized protein OS=Portunus trituberculatus OX=210409 GN=E2C01_031633 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 6.887e-8
Identity = 37/73 (50.68%), Postives = 48/73 (65.75%), Query Frame = 2 
Query: 1715 GKDEVVETPNSDLES-GIVEGYCSDMDNLKNSGYCSDNS----GYMSEGGLGLYAQKMQQRFREGLLAVQESL 1918
            G ++V++   SDLES  +  GY SD D L+N GY S NS    GYMSEGG  LYA ++ QRF+EG+  V ES+
Sbjct:  597 GNNQVLDGDYSDLESVELTNGYMSDGDVLRNVGYRSGNSSDLDGYMSEGGASLYAHRLNQRFKEGMRQVHESM 669
[Close]
BLAST of SMED30015160 vs. TrEMBL
Match: A0A267G5F6 (AAA domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023389g1 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 7.198e-7
Identity = 43/96 (44.79%), Postives = 60/96 (62.50%), Query Frame = 2 
Query: 1766 VEGYCSDMD-NLKNS-GYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKI-----INRKFD---VGRDDLSSTSSEEIEDYLKEL 2023
              GY SD D +++NS GY SD  GYMSE G+G  A++MQQRFREG+ AVQE  + T++  +     ++ + D   V  +D +S SS EI D + EL
Sbjct:  485 ASGYASDCDPHMRNSSGYASDG-GYMSESGIGSCARRMQQRFREGMQAVQEYNRKTTDRYVHKSNELDSETDGPEVDGEDSNSVSSGEISDAVAEL 579
[Close]
BLAST of SMED30015160 vs. TrEMBL
Match: A0A267EEQ0 (AAA domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023844g1 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 4.366e-6
Identity = 46/113 (40.71%), Postives = 62/113 (54.87%), Query Frame = 2 
Query: 1715 GKDEVVETPNSDLESGIVEGYCSDMD--NLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKI-----INRKFD---VGRDDLSSTSSEEIEDYLKEL 2023
            G   +V   N+D       GY SD D     +SGY SD  GYMSE G+G  A++MQQRFREG+ AVQE  + T++        ++ + D   V  +D +S SS EI D + EL
Sbjct:  467 GMAHLVGQQNAD-----ASGYASDCDPHMRSSSGYTSDG-GYMSESGIGSCARRMQQRFREGMQAVQEYNRKTTDRYAHKSNELDSETDMPEVDGEDSNSVSSGEISDAVAEL 573
[Close]
BLAST of SMED30015160 vs. TrEMBL
Match: V3ZKR2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_168331 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 7.354e-6
Identity = 30/56 (53.57%), Postives = 41/56 (73.21%), Query Frame = 2 
Query: 1766 VEGYCSDMDNLKNSGYCSD-NSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTS 1930
            V GY SD D LK +    D +SGYMSEGG  LYA+++QQRFREG+ AV++ ++ +S
Sbjct:  739 VSGYMSDGDILKTNNRVDDWSSGYMSEGGASLYARRLQQRFREGMQAVKDCMQKSS 794
[Close]
BLAST of SMED30015160 vs. Planmine SMEST
Match: SMESG000058374.1 (SMESG000058374.1)

HSP 1 Score: 1390.94 bits (3599), Expect = 0.000e+0
Identity = 756/757 (99.87%), Postives = 756/757 (99.87%), Query Frame = 2 
Query:  158 MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCXXXXXXXXXXXXXXKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNXXXXXXXXXXXXXXXXXVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDXXXXXXXXXXXXXXXXXXEHKPAXXXXXXXXVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRLVG 2428
            MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNR VG
Sbjct:    1 MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRSVG 757
[Close]
BLAST of SMED30015160 vs. Planmine SMEST
Match: SMESG000058374.1 (SMESG000058374.1)

HSP 1 Score: 1377.46 bits (3564), Expect = 0.000e+0
Identity = 749/750 (99.87%), Postives = 749/750 (99.87%), Query Frame = 2 
Query:  179 NSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCXXXXXXXXXXXXXXKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNXXXXXXXXXXXXXXXXXVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDXXXXXXXXXXXXXXXXXXEHKPAXXXXXXXXVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRLVG 2428
            NSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNR VG
Sbjct:    8 NSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRSVG 757
[Close]
BLAST of SMED30015160 vs. Planmine SMEST
Match: SMESG000058374.1 (SMESG000058374.1)

HSP 1 Score: 1377.07 bits (3563), Expect = 0.000e+0
Identity = 750/751 (99.87%), Postives = 750/751 (99.87%), Query Frame = 2 
Query:  176 GNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCXXXXXXXXXXXXXXKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNXXXXXXXXXXXXXXXXXVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDXXXXXXXXXXXXXXXXXXEHKPAXXXXXXXXVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRLVG 2428
            GNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNR VG
Sbjct:   40 GNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRSVG 790
[Close]
BLAST of SMED30015160 vs. Planmine SMEST
Match: SMESG000058374.1 (SMESG000058374.1)

HSP 1 Score: 1347.03 bits (3485), Expect = 0.000e+0
Identity = 738/757 (97.49%), Postives = 738/757 (97.49%), Query Frame = 2 
Query:  158 MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCXXXXXXXXXXXXXXKNTNNNLQHHEISTNKFSTEVIESSARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNXXXXXXXXXXXXXXXXXVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDXXXXXXXXXXXXXXXXXXEHKPAXXXXXXXXVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRLVG 2428
            MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTE                  LSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNR VG
Sbjct:    1 MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGNRQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTE------------------LSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCISHTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIPNPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGKKSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHYTNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNETITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHKPANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYLLSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKDNLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNSGYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKFDVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNSFTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTGKVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSEIKNRSVG 739
The following BLAST results are available for this feature:
BLAST of SMED30015160 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7V6U05.906e-854.10Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A5B7EY736.887e-850.68Uncharacterized protein OS=Portunus trituberculatu... [more]
A0A267G5F67.198e-744.79AAA domain-containing protein OS=Macrostomum ligna... [more]
A0A267EEQ04.366e-640.71AAA domain-containing protein OS=Macrostomum ligna... [more]
V3ZKR27.354e-653.57Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of SMED30015160 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015160 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000058374.10.000e+099.87SMESG000058374.1[more]
SMESG000058374.10.000e+099.87SMESG000058374.1[more]
SMESG000058374.10.000e+099.87SMESG000058374.1[more]
SMESG000058374.10.000e+097.49SMESG000058374.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015160 ID=SMED30015160|Name=SMED30015160|organism=Schmidtea mediterranea sexual|type=transcript|length=2544bp
TGTAATTTGATTTCTTCGTTTTCATTTATTGTCTTCATTTTCTGAAGGAT
GAAATTCAGATAAAATTAATGGTGAAAAAATTAGAAATGCAACTTTATGA
ATTCCATGATAGTAATTATTATGTGAACGTTGATGTGGATTTTTTAACTA
CAATTTCATGTCTACATTAAAGAAAGGCAACAGCGGTATTCCTATATTCG
ATACAAAGCCACTCAGCATCGATAGTGATCTCGCATCGAAATCTGATTTA
ATTCCACAGGGCTCTCCACTAATTTCTCGCCTGCCTAGTTATAAACCTCA
AGGAAATAGACAAAAACTCTCTCTCACTACTAGAAAATGCGTTTCTAGCA
GTGTAACTCCAATTTCCAGTCCAGTGATTCCAAAGAATACTAATAACAAC
TTGCAACACCACGAGATATCAACAAATAAATTTAGCACTGAAGTAATCGA
ATCTTCTGCTCGATTAATTAATGAAATAGATGTTACTTTATCACAGCTAT
CTCGACAATATTACACTGACAATAACAATTTTATTGAAGCCGTTTATACT
CCAGTTAACGATGAAGACGTGACACCGACGTCTCAGCGCAATAACAATTG
TATTTCTCACACGTTCAATACCACACAAGACTTTTGCGACTGCAAAATGT
TAAATAAACTAAAGTTAAAAAAGCCTCAACCTATTGTTTCTTCCTCAGAG
GAATATTCATCACCTATATTAACACCGAAAACCTCTTCAAAACAAAGTCA
TATACCAAATCCTTACAGAGAAAGTGCTAAACAGAAATCCATACAATCTC
GACCTACTAAATTACAATTTACTTATGAACCCAATAAGCCTTTAGATATC
AATCATGAACAATCGCTGATGAATTCCTACAAATCGCTAAAGACTTCAAC
AGGTAAAAAATCTAAATCTTCCTCTGTCAATCGCGCAAAACCTCAAGATA
ACACCCTCAATTCTAATACAACATATACTGAGGCATGGATATACAGTTCT
AGATTACCAATAGTTAGTCATGAGCAAAGCAATCCTGCAAATAATATCAT
TCACTATACAAATAAAGAAGGTCATTGTGTACAAAACTACCCTAAAGTTT
ATTATCCTCTTTATCAAAATCACAGCTCATCTAATTCTGAATTATTTCAA
AAGGCTATCTTTAATCAACATCGTCACAGTAGCAAAACACCTGTTCCAAA
TGAAACCATTACTATTCATAAATATCCACAAAAACTTCCTTTGCCAAGTA
AAAAAGTTAAAAATAACAAAGATGAGCTAAATGCTAATCGTGACAGTGGT
ATTTCATCATCCTCTTGTTCAAAAGAAAGTTGGACAGAATTTTCGTCAGA
ACATAAACCAGCCAATAATAACAATAACAATAATAATGTCATATCGAATC
TTCCTTTACCCCCACCGAGAATATCTTCTCTGGTTCAAGGATCTCATAAT
GCCACAGACAAGTGTCCGACTCTCACAAATCAGTCAAATAATACCAGAGA
TTATCTTTTATCATCTAACGAAGTAAACAAACCAGCGAGTAAAGCTGACA
GGAGAAAAGCTAGAGAGCTGTTTGCATCTGTCAAACAACGAGATGAACAA
AGAATGAAACCAATCAAAGACGATGAGCCAAAACAAACATTAAATGAGAG
TAAAGATAATCTAAAAGAATATAATACTCTAAATGAGGAAATCAAACCAA
AATCAAAGGAAATTGGTAAAGATGAAGTAGTTGAAACGCCCAACAGCGAT
CTAGAAAGTGGCATTGTGGAAGGATACTGTTCTGATATGGATAATTTAAA
AAATAGTGGCTATTGTTCAGATAATAGTGGTTACATGTCAGAAGGAGGTC
TTGGTCTATATGCACAGAAAATGCAACAGAGATTTAGAGAAGGCCTCTTA
GCTGTACAAGAGTCTCTTAAATTGACATCAAACAATAAAATTATCAATCG
AAAATTCGACGTTGGTAGAGATGATTTGTCGTCAACAAGTAGTGAAGAGA
TCGAAGATTATTTGAAAGAACTCAGTAGTCGTTTGGTTTCTCCAGCGATG
TCTGAGTTATCAGAATCGAGGCAATCGAATGAGTTGTTTAATCAACGAAA
AAACAGTTTTACGAGAAATCAGACGAATGATCAAAATACCCAAATTCAAA
GAAAAAACCGTCTTTATCAAGAAAGACTTCAAAAGTATGCATGTGACGTT
GAACCTGCAGCGGACTTTGACATAACTTCAAGTTGTTCAATGGATTTATT
GACAGGGAAAGTAGTTTCTGATGTCGGATCGGAAGAAGACACATCGGAAA
TTAAAGATTATTTACTAAATCGGCGACGAACATCTGACTACCAAGGAATT
CCAAATCAGTATAATTACGGAATTCATTTAAAGAACCAACCTGAAAACAA
AAGCGAAATAAAAAATAGATTAGTTGGAAATAATAATAATAATAATATTA
GCTTTTGCCATTCGATGTCATTAAACAGTAACCATATTTTTATTGTTTGA
ATATTGTTTGGGCAAATACTGTTACTGTTACTTGAATAAAATGA
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protein sequence of SMED30015160-orf-1

>SMED30015160-orf-1 ID=SMED30015160-orf-1|Name=SMED30015160-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=781bp
MSTLKKGNSGIPIFDTKPLSIDSDLASKSDLIPQGSPLISRLPSYKPQGN
RQKLSLTTRKCVSSSVTPISSPVIPKNTNNNLQHHEISTNKFSTEVIESS
ARLINEIDVTLSQLSRQYYTDNNNFIEAVYTPVNDEDVTPTSQRNNNCIS
HTFNTTQDFCDCKMLNKLKLKKPQPIVSSSEEYSSPILTPKTSSKQSHIP
NPYRESAKQKSIQSRPTKLQFTYEPNKPLDINHEQSLMNSYKSLKTSTGK
KSKSSSVNRAKPQDNTLNSNTTYTEAWIYSSRLPIVSHEQSNPANNIIHY
TNKEGHCVQNYPKVYYPLYQNHSSSNSELFQKAIFNQHRHSSKTPVPNET
ITIHKYPQKLPLPSKKVKNNKDELNANRDSGISSSSCSKESWTEFSSEHK
PANNNNNNNNVISNLPLPPPRISSLVQGSHNATDKCPTLTNQSNNTRDYL
LSSNEVNKPASKADRRKARELFASVKQRDEQRMKPIKDDEPKQTLNESKD
NLKEYNTLNEEIKPKSKEIGKDEVVETPNSDLESGIVEGYCSDMDNLKNS
GYCSDNSGYMSEGGLGLYAQKMQQRFREGLLAVQESLKLTSNNKIINRKF
DVGRDDLSSTSSEEIEDYLKELSSRLVSPAMSELSESRQSNELFNQRKNS
FTRNQTNDQNTQIQRKNRLYQERLQKYACDVEPAADFDITSSCSMDLLTG
KVVSDVGSEEDTSEIKDYLLNRRRTSDYQGIPNQYNYGIHLKNQPENKSE
IKNRLVGNNNNNNISFCHSMSLNSNHIFIV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 653..673
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 177..217
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..268
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 426..500
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 459..500
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 426..458
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..217