SMED30015148

Overview
NameSMED30015148
Smed IDSMED30015148
Length (bp)410
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015148 (SMED30015148) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015148 (SMED30015148) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015148 (SMED30015148) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015148 (SMED30015148) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Alignments
SMED30015148 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003047:5812..6371 +1036
Homology
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BLAST of SMED30015148 vs. Planmine SMEST
Match: SMESG000022832.1 (SMESG000022832.1)

HSP 1 Score: 108.997 bits (271), Expect = 1.821e-28
Identity = 52/58 (89.66%), Postives = 57/58 (98.28%), Query Frame = 2 
Query:   32 QKDEDKICKVCDSLMIDGECTGCQDTAEIISCNVNKIEKAFRDSESNMNSGDDFQKAD 205
            +KDEDKICKVCDS MIDGECTGCQDTAEIISCNVN+IEKAF+DS+S+MNSGDDFQKAD
Sbjct:  142 EKDEDKICKVCDSPMIDGECTGCQDTAEIISCNVNEIEKAFKDSKSDMNSGDDFQKAD 199
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BLAST of SMED30015148 vs. Planmine SMEST
Match: SMESG000022828.1 (SMESG000022828.1)

HSP 1 Score: 107.071 bits (266), Expect = 1.148e-27
Identity = 82/162 (50.62%), Postives = 93/162 (57.41%), Query Frame = 2 
Query:   29 LQKDEDKICKVCDSLMIDGECTGCQDT-----------------------------------AEIISCNVNKIEKAFRDSESNMNSGDDFQKADNIKYTDEMKRDQPSVKTTDYYSLNERKCGDQRADRFGKESKTNNIKYKDEMKKDKTNKGNSDQPSVKT 409
            L KD++ +C  CD+L+ DG C  C DT                                    EI   N++KI K+  + + N  +G    KA NIKYT EMKRDQPSVKTTDYYSLNE  C DQRADRFGKESKTNNIKYKDE KK K N  NSDQPSVKT
Sbjct:  142 LDKDDNMVCIYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESKQTIEEIDWENMDKITKS--NVKENKRNGKA-SKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRADRFGKESKTNNIKYKDEKKKHKINNDNSDQPSVKT 300
The following BLAST results are available for this feature:
BLAST of SMED30015148 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015148 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000022832.11.821e-2889.66SMESG000022832.1[more]
SMESG000022828.11.148e-2750.62SMESG000022828.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015148 ID=SMED30015148|Name=SMED30015148|organism=Schmidtea mediterranea sexual|type=transcript|length=410bp
TGATTATGAGTTTTTTACCGCAGCCTCTCTACAAAAAGATGAAGATAAAA
TATGCAAAGTTTGCGACAGTCTCATGATTGATGGAGAATGTACAGGTTGC
CAAGATACGGCAGAAATTATTTCATGCAATGTTAATAAAATTGAGAAGGC
ATTTAGAGATTCGGAGAGTAATATGAATTCGGGAGATGATTTTCAGAAAG
CCGATAATATAAAATATACAGATGAAATGAAAAGAGATCAACCATCCGTT
AAAACAACCGACTATTACAGTTTAAATGAGAGGAAATGTGGGGATCAGAG
AGCTGATAGATTCGGTAAAGAATCAAAAACAAATAATATAAAATATAAAG
ATGAAATGAAAAAGGATAAAACTAATAAAGGTAACTCAGATCAACCATCC
GTTAAAACAC
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protein sequence of SMED30015148-orf-1

>SMED30015148-orf-1 ID=SMED30015148-orf-1|Name=SMED30015148-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp
DYEFFTAASLQKDEDKICKVCDSLMIDGECTGCQDTAEIISCNVNKIEKA
FRDSESNMNSGDDFQKADNIKYTDEMKRDQPSVKTTDYYSLNERKCGDQR
ADRFGKESKTNNIKYKDEMKKDKTNKGNSDQPSVKT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell