SMED30015148
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Alignments
SMED30015148 aligns in the following genomic locations:
Homology
BLAST of SMED30015148 vs. Planmine SMEST
Match: SMESG000022832.1 (SMESG000022832.1) HSP 1 Score: 108.997 bits (271), Expect = 1.821e-28 Identity = 52/58 (89.66%), Postives = 57/58 (98.28%), Query Frame = 2 Query: 32 QKDEDKICKVCDSLMIDGECTGCQDTAEIISCNVNKIEKAFRDSESNMNSGDDFQKAD 205 +KDEDKICKVCDS MIDGECTGCQDTAEIISCNVN+IEKAF+DS+S+MNSGDDFQKAD Sbjct: 142 EKDEDKICKVCDSPMIDGECTGCQDTAEIISCNVNEIEKAFKDSKSDMNSGDDFQKAD 199
BLAST of SMED30015148 vs. Planmine SMEST
Match: SMESG000022828.1 (SMESG000022828.1) HSP 1 Score: 107.071 bits (266), Expect = 1.148e-27 Identity = 82/162 (50.62%), Postives = 93/162 (57.41%), Query Frame = 2 Query: 29 LQKDEDKICKVCDSLMIDGECTGCQDT-----------------------------------AEIISCNVNKIEKAFRDSESNMNSGDDFQKADNIKYTDEMKRDQPSVKTTDYYSLNERKCGDQRADRFGKESKTNNIKYKDEMKKDKTNKGNSDQPSVKT 409 L KD++ +C CD+L+ DG C C DT EI N++KI K+ + + N +G KA NIKYT EMKRDQPSVKTTDYYSLNE C DQRADRFGKESKTNNIKYKDE KK K N NSDQPSVKT Sbjct: 142 LDKDDNMVCIYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESKQTIEEIDWENMDKITKS--NVKENKRNGKA-SKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRADRFGKESKTNNIKYKDEKKKHKINNDNSDQPSVKT 300 The following BLAST results are available for this feature:
BLAST of SMED30015148 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30015148 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30015148 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30015148 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30015148 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015148 ID=SMED30015148|Name=SMED30015148|organism=Schmidtea mediterranea sexual|type=transcript|length=410bpback to top protein sequence of SMED30015148-orf-1 >SMED30015148-orf-1 ID=SMED30015148-orf-1|Name=SMED30015148-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp DYEFFTAASLQKDEDKICKVCDSLMIDGECTGCQDTAEIISCNVNKIEKAback to top Annotated Terms
The following terms have been associated with this transcript:
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