Voltage-dependent calcium channel type A subunit alpha-1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30015099 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Human
Match: CACNA1S (calcium voltage-gated channel subunit alpha1 S [Source:HGNC Symbol;Acc:HGNC:1397]) HSP 1 Score: 1067.37 bits (2759), Expect = 0.000e+0 Identity = 672/1546 (43.47%), Postives = 966/1546 (62.48%), Query Frame = 2
Query: 176 PNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV---------------DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS-------------------EAKIQDIDDSIIEELNMY---DKPKDKKQPIKESPVIVENPPAEGIIVRKKILP---YSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P P R+LF + N +R++C ++E + FE +++LTI ANCV+LA+ + +P+ D + + +E FL +F+ EA +KIIA GF+ ++AYL+S WN+LDF +V +G+ TV L+ V D++ RA RVLRP +LVSGVPSLQVVL SI KAM PL I LLVLF V+I+AIIGLE+++G H TC+ + + + P + T G C+ W GP G+ +FDN G++MLTV+QCITMEGWTDVLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R F KLRE Q+ + L GYM+W+ T E++ E+ + E K L + T+ E +K I +W ++ R +K++ FYW VI +V LNT +A EH+ QP +L R A V LS+F TE+ +K++G G+R YF S FN FD VV ++E + G S LRCIRLLR+ K+T+YW+SL NLV SLL+S+RSI SLLLLL LFI I LLGMQLFGG ++FE + NFD FP A+I+VF +LTGE+W +MYNGI M M+ YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++++ R+ ++ + S + KI + + ++ E + Y D P D ++ E P+ P + +++K +P SS FIF P N IR +CH + N +F FI++ I SS ALAAEDP+ +S+RN++L+ FD+ FTSVF +E++LK+ G LH GS+ R+ +N++D++VV +++ S S ++ +K LRV+RVLRPL+ INR LK V + + ++ + NI++V L QF+F I VQLF GKFF C DLSK +E C+GY+Y + D M I+ + R W + F++DN++++M++LFTV+T EGWP +L +I + + GPI N + E+AIF+I++ I+ FF +NIFV +IVTFQEQGE++ + ELDKN+ +C+ +A+ +P Y+P KN Y+VW +V S FEYL+ +I L++I L ++ + QS Q + + LN+AFTIIFT E ILK++A++ + +F DPWN+FDF+ V+GS+ DV LSE+ + + I + F RLFRVMRLIK+L R +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I L D TQIN+NNNF+ F +LLLFRCATGE+W I++ GK C + G ++ CG++F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ +WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + + ++ELR + K+W + + P+ +TVGK YA LI +++R F K
Sbjct: 25 PRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWI-TQGEVMDVEDFR--EGKLSLDEGGSDTESLYE---------------IAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGI---MAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIP--KNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYN----------------QSEQMNHISDI--LNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDESARISS-AFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPG-----EEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIK--TEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDE--VTVGKFYATFLIQEHFRKFMK 1519 HSP 2 Score: 100.523 bits (249), Expect = 2.482e-20 Identity = 87/314 (27.71%), Postives = 150/314 (47.77%), Query Frame = 2
Query: 2234 IEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIF--NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFF---------YSKNLNT------IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGK-----FFYCNDLSKRIK--------ETCKGYFYTFNDQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
+E + D+ KKQP K P I+ PP +LF N +R+ C + + F+ I++ IFA+ +ALA P+ N+ N LE + F VF +E +K+I G + H +Y+R WN++D +V + VF+ ++ +++ +K+LR RVLRPL+ ++ VP L+ V +I ++ +F+I ++ + I+ +I ++LF GK +F D+ ++ T G T N G + + N+ ++DN SM+T++ T EGW DVL
Sbjct: 1 MEPSSPQDEGLRKKQPKKPVPEILPRPP-------------RALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTIN---GSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVL 298
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Human
Match: CACNA1S (calcium voltage-gated channel subunit alpha1 S [Source:HGNC Symbol;Acc:HGNC:1397]) HSP 1 Score: 1055.82 bits (2729), Expect = 0.000e+0 Identity = 672/1565 (42.94%), Postives = 967/1565 (61.79%), Query Frame = 2
Query: 176 PNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV---------------DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS-------------------EAKIQDIDDSIIEELNMY---DKPKDKKQPIKESPVIVENPPAEGIIVRKKILP---YSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKS-------------------EESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P P R+LF + N +R++C ++E + FE +++LTI ANCV+LA+ + +P+ D + + +E FL +F+ EA +KIIA GF+ ++AYL+S WN+LDF +V +G+ TV L+ V D++ RA RVLRP +LVSGVPSLQVVL SI KAM PL I LLVLF V+I+AIIGLE+++G H TC+ + + + P + T G C+ W GP G+ +FDN G++MLTV+QCITMEGWTDVLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R F KLRE Q+ + L GYM+W+ T E++ E+ + E K L + T+ E +K I +W ++ R +K++ FYW VI +V LNT +A EH+ QP +L R A V LS+F TE+ +K++G G+R YF S FN FD VV ++E + G S LRCIRLLR+ K+T+YW+SL NLV SLL+S+RSI SLLLLL LFI I LLGMQLFGG ++FE + NFD FP A+I+VF +LTGE+W +MYNGI M M+ YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++++ R+ ++ + S + KI + + ++ E + Y D P D ++ E P+ P + +++K +P SS FIF P N IR +CH + N +F FI++ I SS ALAAEDP+ +S+RN++L+ FD+ FTSVF +E++LK+ G LH GS+ R+ +N++D++VV +++ S S ++ +K LRV+RVLRPL+ INR LK V + + ++ + NI++V L QF+F I VQLF GKFF C DLSK +E C+GY+Y + D M I+ + R W + F++DN++++M++LFTV+T EGWP +L +I + + GPI N + E+AIF+I++ I+ FF +NIFV +IVTFQEQGE++ + ELDKN+ +C+ +A+ +P Y+P KN Y+VW +V S FEYL+ +I L++I L ++ + QS Q + + LN+AFTIIFT E ILK++A++ + +F DPWN+FDF+ V+GS+ DV LSE+ + + + I + F RLFRVMRLIK+L R +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I L D TQIN+NNNF+ F +LLLFRCATGE+W I++ GK C + G ++ CG++F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ +WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + + ++ELR + K+W + + P+ +TVGK YA LI +++R F K
Sbjct: 25 PRPPRALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLDEDLRGYMSWI-TQGEVMDVEDFR--EGKLSLDEGGSDTESLYE---------------IAGLNKIIQFIRHWRQWNRIFRWKCHDIVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTRLQDIANRVLLSLFTTEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRIFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIVIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWTSMMYNGI---MAYGGPSYPGMLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEEKKRRKMSKGLPDKSEEEKSTMAKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPLSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILKHFDIGFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNMLDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCMFVAISTIGNIVLVTTLLQFMFACIGVQLFKGKFFRCTDLSKMTEEECRGYYYVYKDGDPMQIELRHREWVHSDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNAEDVGPIYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIP--KNPYQYQVWYIVTSSYFEYLMFALIMLNTICLGMQHYN----------------QSEQMNHISDI--LNVAFTIIFTLEMILKLMAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISS-AFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPG-----EEYTCGTNFAYYYFISFYMLCAFLVINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIK--TEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDE--VTVGKFYATFLIQEHFRKFMK 1538 HSP 2 Score: 100.523 bits (249), Expect = 2.873e-20 Identity = 89/314 (28.34%), Postives = 147/314 (46.82%), Query Frame = 2
Query: 2234 IEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIF--NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY--------------SKNLN-TIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGK-----FFYCNDLSKRIK--------ETCKGYFYTFNDQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
+E + D+ KKQP K P I+ PP +LF N +R+ C + + F+ I++ IFA+ +ALA P+ N+ N LE + F VF +E +K+I G + H +Y+R WN++D +V + VF+ SK +K+LR RVLRPL+ ++ VP L+ V +I ++ +F+I ++ + I+ +I ++LF GK +F D+ ++ T G T N G + + N+ ++DN SM+T++ T EGW DVL
Sbjct: 1 MEPSSPQDEGLRKKQPKKPVPEILPRPP-------------RALFCLTLENPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFTIVFLGVFTVILEQVNVIQSHTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEEPSPCARTGSGRRCTIN---GSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVL 298
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Human
Match: CACNA1C (calcium voltage-gated channel subunit alpha1 C [Source:HGNC Symbol;Acc:HGNC:1390]) HSP 1 Score: 1053.12 bits (2722), Expect = 0.000e+0 Identity = 677/1572 (43.07%), Postives = 946/1572 (60.18%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWK----TDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVF-------LAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS--EAKIQ------DIDDSIIEELNMYD-KPKDK--KQPIKES-------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY N A W +YFV LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD VV ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVF LA A + E +E + E+ + + G E KI+ D + ++NM D +P + K P PV P + +++K +P S+ FIF +N R CH + N F I+ I SS++LAAEDPV S RN +L +FD+ FT++F +E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1633 HSP 2 Score: 104.375 bits (259), Expect = 1.716e-21 Identity = 74/260 (28.46%), Postives = 135/260 (51.92%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E LK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 873 PMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1124
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Human
Match: CACNA1C (calcium voltage-gated channel subunit alpha1 C [Source:HGNC Symbol;Acc:HGNC:1390]) HSP 1 Score: 1053.12 bits (2722), Expect = 0.000e+0 Identity = 677/1572 (43.07%), Postives = 946/1572 (60.18%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWK----TDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVF-------LAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS--EAKIQ------DIDDSIIEELNMYD-KPKDK--KQPIKES-------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY N A W +YFV LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD VV ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVF LA A + E +E + E+ + + G E KI+ D + ++NM D +P + K P PV P + +++K +P S+ FIF +N R CH + N F I+ I SS++LAAEDPV S RN +L +FD+ FT++F +E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1633 HSP 2 Score: 104.375 bits (259), Expect = 1.716e-21 Identity = 74/260 (28.46%), Postives = 135/260 (51.92%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E LK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 873 PMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1124
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Human
Match: CACNA1C (calcium voltage-gated channel subunit alpha1 C [Source:HGNC Symbol;Acc:HGNC:1390]) HSP 1 Score: 1051.58 bits (2718), Expect = 0.000e+0 Identity = 679/1572 (43.19%), Postives = 944/1572 (60.05%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWK----TDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVF-------LAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS--EAKIQ------DIDDSIIEELNMYD-KPKDK--KQPIKES-------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY N A W +YFV LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD VV ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVF LA A + E +E + E+ + + G E KI+ D + ++NM D +P + K P PV P + +++K +P S+ FIF +N R CH + N F I+ I SS++LAAEDPV S RN +L D FTS+F LE+ILK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1633 HSP 2 Score: 109.768 bits (273), Expect = 4.824e-23 Identity = 75/260 (28.85%), Postives = 136/260 (52.31%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E ILK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 873 PMPEASAFFIFSSNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1124
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45]) HSP 1 Score: 1231.85 bits (3186), Expect = 0.000e+0 Identity = 773/1489 (51.91%), Postives = 1022/1489 (68.64%), Query Frame = 2
Query: 311 LEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV----------NLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTS-------CNA-GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKT-KEWLERKMRQDE-----------YICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYS-------KNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGI-QAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLS-PQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNK--ASYPLTVGKIYAGMLIMKNWR 4654
+E HLP+ D+ + + EP F+GIF E +LK+IA GF + K +YL+S WNI+DFIVV G++T+ N D+VDLRT RA+RVLRP KLVSG+PSLQVVL SI+ AMAPLLQIGLLVLFA++IFAIIGLE Y G FH+ C+ + + P +N + C+ G TC W GP +G+ +FDNIG+AM+TVFQCITMEGWT V+Y TN + S +N YF+ LIVLGSFFMLNLVLGVLSGEFAKERERVE RR FLKLR Q+ E L+GY+ W+ TAEE+I E++ EEK +M+ R++ + L++ +Q E E D+ + K + + K+I ++R ++ +KTQ FYW VITLV LNT CVA EHYGQP++ FL YAE+VFL IF+ E+ +K+F G R YF SKFN FD +V+ S E I+ + SFG S +R +RLLR+ KLT YW SLRNLV SL++SMRSIISLL LL LFI I LLGMQLFGG FNF P +FDTFP A+ITVF ILTGE+WNEVMY I SQ + YS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEE + K E+ E E +E+ + G I D++ ++ + D + E P G K ++PYSS+F P N R + H + ++F++ +M VI SS++LAAEDPV + RN++L++ D FT VF EM+LK+ID G++LHPGSY RD WNI+D IVV A+F+F ++ KNLNTIKSLRV+RVLRPLKTI R+PKLKAVF+ +++S+KNVFNILIV+ LFQFIF VIAVQLF+GKFF+C D +++ TC G F+ +++Q + ++R W+ FNYDN +N+M+TLF VTTGEGWP + +NS+ T+ ++GP ++ E+A+FY++FFIVFPFFFVNIFVALII+TFQEQGE++ S+ +LDKN+ +CIDFA+N +P S +MP +KNS Y++W LV SPPFEY IM +I +++ L++K + +N F E L+ N A T +FT E ILK++A+ + +F+D WN FDFVTV+GS+TD ++E +LGFLRLFR RLI++L +GY+IRILLWTF+QSFKALP+VCLLIG+LFFIY I+GMQ+FGNI LN T+IN++NNF++F ++LLFRCATGE W IM+ GK CA G ++ + + CGS+ SY YF +F+ +++FLMLNLFVAVIMDNFDYLTRD SILGPHHLDE++R+WA++DP TG +++ +MY+M++ + PPVGFGK CPYR A LIRMNM + V+F +TLFALIRESL IKM V E+D D+ELR+ + K+WP K+N +D +P N LTVGKIYAG+LI++N+R
Sbjct: 1 MEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEI--ENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVY-GGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSG---GWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEADEKANEIEEESEELDEQYQE-GDHCTI-DMEGKTAGDMCAVARAMDDL----DEECEEEESPFGG---PKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMK---------YYNNPLFYEE---------ILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGH-----FVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCA----RAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYR 1447 HSP 2 Score: 97.8265 bits (242), Expect = 5.836e-20 Identity = 78/271 (28.78%), Postives = 127/271 (46.86%), Query Frame = 2
Query: 161 FVGQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGD-RTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQA-------DTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDV 934
F G KP P S+F S N R ++ ++FE +V+ I + VSLA E + + + R ++ M + F G+F E +LK+I G ++ +Y + WNILD IVVT L + +++ R +RVLRP K + +P L+ V ++ ++ + I ++ IFA+I ++++ G F + TD F T C+ Q N + + + PF N+DN AMLT+F T EGW +
Sbjct: 688 FGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYM---DYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFF---FCTDKNRKFANT---CHGQFFVYDNQNDPPRVEQREWRLRPF----NYDNTINAMLTLFVVTTGEGWPGI 945 HSP 3 Score: 72.0182 bits (175), Expect = 4.246e-12 Identity = 73/310 (23.55%), Postives = 129/310 (41.61%), Query Frame = 2
Query: 188 RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVL------------------YQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
RSLF+ + N + L+ FEY ++ I N + L ++ + + F + +FT E+ILKI+A G Y + WN DF+ V +G IT L + LR FRA R++R L+ +++++L + +++ L + LL+ I+AI+G++V+ I+ N + + + N NF + A++ +F+C T EGW D++ ++ + S + YF + L SF MLNL + V+
Sbjct: 1024 RSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYY---NNPLFYEEILRLFNTALTAVFTVESILKILAFGV----RNYFRDGWNRFDFVTV-VGSITDALVTEFGGHFVSLGFLRLFRAARLIR---LLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEIN--------------RHN---------------------NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVI 1287
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Celegans
Match: egl-19 (Voltage-dependent L-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:W6RY76]) HSP 1 Score: 1026.54 bits (2653), Expect = 0.000e+0 Identity = 662/1535 (43.13%), Postives = 940/1535 (61.24%), Query Frame = 2
Query: 185 DRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV---DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPY-----------------WDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN-----WIKYIHEMRPV-------VKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNF--EQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYF--YTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLT----TGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
+RSL S NN IR+ C ++E + FE+L++ I ANC++LA+ P D + T + IE +F+ +FT E +LKI+A+GF+ +AYL++ WNILDFI+V IGL++ L ++ D++ RA RVLRP +LVSGVPSLQVVL +I++AM PLL I LLVLF +LI+AIIGLE++ G H+TC P Q+ P + + F C+P W GP G+ NFDN G AMLTVFQC+++EGWTDV+Y N A +W +YFV L++LGSFF+LNLVLGVLSGEF+KERE+ R F K RE Q+ E L GY++W+ AE++ E + ++DE + + ++ D++ + +E+ W + + + + +K+Q FYW VI LVLLNT + EHYGQ ++LD F A F+ +F E+ +K++ G Y S+FN FD VV S++E + + + G S LR RLLR+ K+T+YW+SLRNLV SLL+S+RSIISLLLLL LFI I LLGMQ+FGG FNF +Q KP NFDTF A++TVF ILTGE+WN VMY+GI S +I Y++++ I GNY LLNVFLAIAVDNLA+A L+ AE+EE ++E + + E + D+P+ ++ P + PA + K I SSLFI N R C+ V N +F ++ I SS LAAEDP+ NS RN +L +FD FTSVF +E+ LKVI GLV H GS+ R+ +N++D++VV +++ SF + ++ +K LRV+RVLRPL+ INR LK V + +I +VK + NI++V + QF+F +I VQLF G FF CNDLSK + C+G + Y D +KKR+W N FN+DN+ ++MI+LF V+T EGWP +L +I + ++GPI N +Q +A+F+I F IV FF +NIFV +IVTFQ +GE + + ELDKN+ KCI+FA+ KP RY+P +N + Y+VW V S FEY+I +II +++++L K + R F+ DF L N+ FT +F E +LK++A K + D WN+FD + V+GS D+ ++ T + ++ F RLFRVMRL+K+L RG IR LLWTF++SF+ALP+V LLI LLFFIY +IGMQ FG + L+D T I++NNNF +F +L+LFR ATGE+W IM+ + C ++ GG+ + CG++F+YPYFI+F ++ +FL++NLFVAVIMDNFDYLTRD SILGPHHL+E+VRLW+E+DP G + D+ +++K+ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+I + ID +++LR + ++W +K + +D +P +TVGK YA LI +R F K
Sbjct: 65 ERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCI---DPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDI-----------------------EPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGT---LGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEI----------------EGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPRRMSEVPAASTV--KPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIP--RNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPS--SRGFE-------------DF---LDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNP--GGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGL----NESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIY--TEGNIDEANEQLRSAIKRIWKRTHK-DLLDEVVPPAGKEDDVTVGKFYATFLIQDYFRRFKK 1523 HSP 2 Score: 153.68 bits (387), Expect = 7.414e-37 Identity = 167/671 (24.89%), Postives = 302/671 (45.01%), Query Frame = 2
Query: 173 KPNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHL-PQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ-SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY------QTNKA---TDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQS--FGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYN-----GIRSQMKTSN--------SRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
KP P SLFI S N R C ++ +F V+ I+ + LA E L R I+ + F +FT E LK+I G + K ++ ++ +N+LD +VV + L + L+ D + ++ R +RVLRP + ++ L+ V+ +I A+ + I L+ +FAIIG+++++G F C D + +A + Y + + K W F NFDN+G AM+++F T EGW +LY + +K Q L+F+ I++ +FFM+N+ +G + ++ F+N+ E E EL KN++K E+ + YI N ++Y V F+ ++ F + + ++++NT +A +HY + + FL +F +F E +KI ++Y ++NVFD +VV S ++ + +NP + + R R++RL+KL +R L+++ + S +++ + LL+ L FI ++GMQ FG D + + NF +FP AI+ +F TGE W ++M + +R + + SR + YF+ + ++ ++N+F+A+ +D
Sbjct: 770 KPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYF---DYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFF-LC--NDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDF---NFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFV------------------------------------------IVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIP----------------RNRLQY------RVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNN-NFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMD 1366 HSP 3 Score: 108.227 bits (269), Expect = 5.228e-23 Identity = 85/296 (28.72%), Postives = 149/296 (50.34%), Query Frame = 2
Query: 2270 KKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDP--VIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSK---NLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFY-CND-----LSKRIKETC----KGYFYTFNDQ-----MGIQ---AKKRIW---QRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
KK+P + P+ N ++ R + SL +N IR++C + + F+ I+ +I A+ +ALA P + +N LLE + F VF +E +LK++ +G + HP +Y+R+ WNI+D I+V+I + S SK +K+LR RVLRPL+ ++ VP L+ V I+ ++ + +I ++ + I+ +I ++LF GK C D L+++ C +G + MG++ + W N+DN +M+T+F + EGW DV+
Sbjct: 46 KKRPAQRKPLRQTN-----VVERSE----RSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVM 332
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Celegans
Match: egl-19 (Voltage-dependent L-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:W6RY76]) HSP 1 Score: 1026.16 bits (2652), Expect = 0.000e+0 Identity = 661/1535 (43.06%), Postives = 941/1535 (61.30%), Query Frame = 2
Query: 185 DRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV---DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPY-----------------WDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN-----WIKYIHEMRPV-------VKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNF--EQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQ--AKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLT----TGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
+RSL S NN IR+ C ++E + FE+L++ I ANC++LA+ P D + T + IE +F+ +FT E +LKI+A+GF+ +AYL++ WNILDFI+V IGL++ L ++ D++ RA RVLRP +LVSGVPSLQVVL +I++AM PLL I LLVLF +LI+AIIGLE++ G H+TC P Q+ P + + F C+P W GP G+ NFDN G AMLTVFQC+++EGWTDV+Y N A +W +YFV L++LGSFF+LNLVLGVLSGEF+KERE+ R F K RE Q+ E L GY++W+ AE++ E + ++DE + + ++ D++ + +E+ W + + + + +K+Q FYW VI LVLLNT + EHYGQ ++LD F A F+ +F E+ +K++ G Y S+FN FD VV S++E + + + G S LR RLLR+ K+T+YW+SLRNLV SLL+S+RSIISLLLLL LFI I LLGMQ+FGG FNF +Q KP NFDTF A++TVF ILTGE+WN VMY+GI S +I Y++++ I GNY LLNVFLAIAVDNLA+A L+ AE+EE ++E + + E + D+P+ ++ P + PA + K I SSLFI N R C+ V N +F ++ I SS LAAEDP+ NS RN +L +FD FTSVF +E+ LKVI GLV H GS+ R+ +N++D++VV +++ SF + ++ +K LRV+RVLRPL+ INR LK V + +I +VK + NI++V + QF+F +I VQLF G FF CNDLSK + C+G + + D + +KKR+W N FN+DN+ ++MI+LF V+T EGWP +L +I + ++GPI N +Q +A+F+I F IV FF +NIFV +IVTFQ +GE + + ELDKN+ KCI+FA+ KP RY+P +N + Y+VW V S FEY+I +II +++++L K + R F+ DF L N+ FT +F E +LK++A K + D WN+FD + V+GS D+ ++ T + ++ F RLFRVMRL+K+L RG IR LLWTF++SF+ALP+V LLI LLFFIY +IGMQ FG + L+D T I++NNNF +F +L+LFR ATGE+W IM+ + C ++ GG+ + CG++F+YPYFI+F ++ +FL++NLFVAVIMDNFDYLTRD SILGPHHL+E+VRLW+E+DP G + D+ +++K+ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+I + ID +++LR + ++W +K + +D +P +TVGK YA LI +R F K
Sbjct: 65 ERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCI---DPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDI-----------------------EPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGT---LGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEI----------------EGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPRRMSEVPAASTV--KPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIP--RNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPS--SRGFE-------------DF---LDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNP--GGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGL----NESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIY--TEGNIDEANEQLRSAIKRIWKRTHK-DLLDEVVPPAGKEDDVTVGKFYATFLIQDYFRRFKK 1523 HSP 2 Score: 153.68 bits (387), Expect = 6.715e-37 Identity = 167/671 (24.89%), Postives = 302/671 (45.01%), Query Frame = 2
Query: 173 KPNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHL-PQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ-SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY------QTNKA---TDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQS--FGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYN-----GIRSQMKTSN--------SRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
KP P SLFI S N R C ++ +F V+ I+ + LA E L R I+ + F +FT E LK+I G + K ++ ++ +N+LD +VV + L + L+ D + ++ R +RVLRP + ++ L+ V+ +I A+ + I L+ +FAIIG+++++G F C D + +A + Y + + K W F NFDN+G AM+++F T EGW +LY + +K Q L+F+ I++ +FFM+N+ +G + ++ F+N+ E E EL KN++K E+ + YI N ++Y V F+ ++ F + + ++++NT +A +HY + + FL +F +F E +KI ++Y ++NVFD +VV S ++ + +NP + + R R++RL+KL +R L+++ + S +++ + LL+ L FI ++GMQ FG D + + NF +FP AI+ +F TGE W ++M + +R + + SR + YF+ + ++ ++N+F+A+ +D
Sbjct: 770 KPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYF---DYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFF-LC--NDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDF---NFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFV------------------------------------------IVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIP----------------RNRLQY------RVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNN-NFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAVIMD 1366 HSP 3 Score: 108.227 bits (269), Expect = 4.965e-23 Identity = 85/296 (28.72%), Postives = 149/296 (50.34%), Query Frame = 2
Query: 2270 KKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDP--VIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSK---NLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFY-CND-----LSKRIKETC----KGYFYTFN-----DQMGIQ---AKKRIW---QRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
KK+P + P+ N ++ R + SL +N IR++C + + F+ I+ +I A+ +ALA P + +N LLE + F VF +E +LK++ +G + HP +Y+R+ WNI+D I+V+I + S SK +K+LR RVLRPL+ ++ VP L+ V I+ ++ + +I ++ + I+ +I ++LF GK C D L+++ C +G + MG++ + W N+DN +M+T+F + EGW DV+
Sbjct: 46 KKRPAQRKPLRQTN-----VVERSE----RSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVM 332
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45]) HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0 Identity = 418/783 (53.38%), Postives = 569/783 (72.67%), Query Frame = 2
Query: 2339 KKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYS-------KNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGI-QAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLS-PQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNK--ASYPLTVGKIYAGMLIMKNWR 4654
K ++PYSS+F P N R + H + ++F++ +M VI SS++LAAEDPV + RN++L++ D FT VF EM+LK+ID G++LHPGSY RD WNI+D IVV A+F+F ++ KNLNTIKSLRV+RVLRPLKTI R+PKLKAVF+ +++S+KNVFNILIV+ LFQFIF VIAVQLF+GKFF+C D +++ TC G F+ +++Q + ++R W+ FNYDN +N+M+TLF VTTGEGWP + +NS+ T+ ++GP ++ E+A+FY++FFIVFPFFFVNIFVALII+TFQEQGE++ S+ +LDKN+ +CIDFA+N +P S +MP +KNS Y++W LV SPPFEY IM +I +++ L++K + +N F E L+ N A T +FT E ILK++A+ + +F+D WN FDFVTV+GS+TD ++E +LGFLRLFR RLI++L +GY+IRILLWTF+QSFKALP+VCLLIG+LFFIY I+GMQ+FGNI LN T+IN++NNF++F ++LLFRCATGE W IM+ GK CA G ++ + + CGS+ SY YF +F+ +++FLMLNLFVAVIMDNFDYLTRD SILGPHHLDE++R+WA++DP TG +++ +MY+M++ + PPVGFGK CPYR A LIRMNM + V+F +TLFALIRESL IKM V E+D D+ELR+ + K+WP K+N +D +P N LTVGKIYAG+LI++N+R
Sbjct: 970 KPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMK---------YYNNPLFYEE---------ILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGH-----FVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCA----RAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYR 1725 HSP 2 Score: 541.191 bits (1393), Expect = 6.667e-160 Identity = 358/691 (51.81%), Postives = 460/691 (66.57%), Query Frame = 2
Query: 131 YLARQTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV----------NLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTS-------CNA-GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQK-TKEWLERKMRQDE------YICXXXXXXXXXXXXXXXIENWI-------------KYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
++ + TLS + ++ P SLFIF+E+NIIRR+ K +IE FEY ++LTII NCV L++E HLP+ D+ + + EP F+GIF E +LK+IA GF + K +YL+S WNI+DFIVV G++T+ N D+VDLRT RA+RVLRP KLVSG+PSLQVVL SI+ AMAPLLQIGLLVLFA++IFAIIGLE Y G FH+ C+ + + P +N + C+ G TC W GP +G+ +FDNIG+AM+TVFQCITMEGWT V+Y TN + S +N YF+ LIVLGSFFMLNLVLGVLSGEFAKERERVE RR FLKLR Q+ E L+GY+ W+ TAEE+I E++ EEK +M+ R++ + L++ +Q + + + ++ +E+ K+I ++R ++ +KTQ FYW VITLV LNT CVA EHYGQP++ FL YAE+VFL IF+ E+ +K+F G R YF SKFN FD +V+ S E I+ + SFG S +R +RLLR+ KLT YW SLRNLV SL++SMRSIISLL LL LFI I LLGMQLFGG FNF P +FDTFP A+ITVF ILTGE+WNEVMY I SQ + YS YF+++V+FGNYTLLNVFLAIAVD
Sbjct: 212 FVRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEI--ENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVY-GGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSG---GWPYSIYFIVLVLFGNYTLLNVFLAIAVD 895 HSP 3 Score: 109.383 bits (272), Expect = 2.316e-23 Identity = 100/342 (29.24%), Postives = 158/342 (46.20%), Query Frame = 2
Query: 2207 KIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAE-----GIIVRKKILPY---------SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLR--NRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFF-YSKNLNT---------IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFF-YCNDLSKRIKETCKGYFYTFN----------DQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL---KNSIYATYS 3106
++ D+ D L M + +Q S + E E G VRK L SSLFIF N IRR + F+ FI++ I + + L+ E + N + + LE + F +F LE +LKVI G LH GSY+R WNIMD IVV+ V + +S T +++LR +RVLRPLK ++ +P L+ V ++I+ ++ + I ++ + IF +I ++ +SG F C + I+ + N D G ++ NY ++DNI +MIT+F T EGW V+ +S+ +TY+
Sbjct: 166 RVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYN 507 HSP 4 Score: 98.2117 bits (243), Expect = 5.988e-20 Identity = 78/271 (28.78%), Postives = 126/271 (46.49%), Query Frame = 2
Query: 161 FVGQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGD-RTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDV 934
F G KP P S+F S N R ++ ++FE +V+ I + VSLA E + + + R ++ M + F G+F E +LK+I G ++ +Y + WNILD IVVT L +++ R +RVLRP K + +P L+ V ++ ++ + I ++ IFA+I ++++ G F + TD F T C+ Q N + + + PF N+DN AMLT+F T EGW +
Sbjct: 966 FGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYM---DYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFF---FCTDKNRKFANT---CHGQFFVYDNQNDPPRVEQREWRLRPF----NYDNTINAMLTLFVVTTGEGWPGI 1223 HSP 5 Score: 73.9442 bits (180), Expect = 1.404e-12 Identity = 69/252 (27.38%), Postives = 127/252 (50.40%), Query Frame = 2
Query: 1376 HFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPK-QSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQ-MKTSNSRDF---------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
+ + PF + ++T++ NT + +++Y P F + L ++F E +KI FG+R+YFR +N FDF+ V S+ +A+ S GF L L L Y ++R L+++ + S +++ + LL+G+ FI ++GMQ+FG + E + NF +F NA+I +F TGE W ++M ++ + + S + S + +YF V ++ +LN+F+A+ +D
Sbjct: 1319 RLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGY--TIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHN-NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMD 1567 HSP 6 Score: 73.1738 bits (178), Expect = 1.869e-12 Identity = 73/310 (23.55%), Postives = 129/310 (41.61%), Query Frame = 2
Query: 188 RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVL------------------YQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
RSLF+ + N + L+ FEY ++ I N + L ++ + + F + +FT E+ILKI+A G Y + WN DF+ V +G IT L + LR FRA R++R L+ +++++L + +++ L + LL+ I+AI+G++V+ I+ N + + + N NF + A++ +F+C T EGW D++ ++ + S + YF + L SF MLNL + V+
Sbjct: 1302 RSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYY---NNPLFYEEILRLFNTALTAVFTVESILKILAFGV----RNYFRDGWNRFDFVTV-VGSITDALVTEFGGHFVSLGFLRLFRAARLIR---LLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEIN--------------RHN---------------------NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVI 1565 HSP 7 Score: 71.633 bits (174), Expect = 6.547e-12 Identity = 62/223 (27.80%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 3527 LKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIM-IDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
LKY F IF E +LK+ A + +F +N FD V ++GS +V +E+ + +R R++R+ K+ S+R L+ + + S +++ + L+ L I+ ++GMQ+FG N T ++ +F F + L+ +F+ TGE W+ +M + ES G + +GG S YFI +L + +LN+F+A+ +DN
Sbjct: 699 LKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSF-----GISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGG-RFNFPT-MHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQ-----GGIYSGGWPYS------------IYFIVLVLFGNYTLLNVFLAIAVDNL 897 HSP 8 Score: 70.0922 bits (170), Expect = 1.988e-11 Identity = 83/342 (24.27%), Postives = 144/342 (42.11%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDV--FLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF------------GNIE------------------LNDDTQ--------INQN---NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
P+S ++ A N + ++ PFEY I++ I + + L ++ H LP +++ K L E L+ F IF EC+LK+IA+ F L + + WNI DF+ V+ + + F ++ + +L LR RV+R +K++ S++++L + + + L + LL+ I+ IIG++ + G IE N D + I N +F N ++ +F+C T E W +M T D L GS +++ YFI I++ +F MLNL + V+ F
Sbjct: 229 PSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQH--LP----KNDKKALSEW---------LERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYT--------------------NDSL-GSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEF 534
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45]) HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0 Identity = 418/783 (53.38%), Postives = 569/783 (72.67%), Query Frame = 2
Query: 2339 KKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYS-------KNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGI-QAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLS-PQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNK--ASYPLTVGKIYAGMLIMKNWR 4654
K ++PYSS+F P N R + H + ++F++ +M VI SS++LAAEDPV + RN++L++ D FT VF EM+LK+ID G++LHPGSY RD WNI+D IVV A+F+F ++ KNLNTIKSLRV+RVLRPLKTI R+PKLKAVF+ +++S+KNVFNILIV+ LFQFIF VIAVQLF+GKFF+C D +++ TC G F+ +++Q + ++R W+ FNYDN +N+M+TLF VTTGEGWP + +NS+ T+ ++GP ++ E+A+FY++FFIVFPFFFVNIFVALII+TFQEQGE++ S+ +LDKN+ +CIDFA+N +P S +MP +KNS Y++W LV SPPFEY IM +I +++ L++K + +N F E L+ N A T +FT E ILK++A+ + +F+D WN FDFVTV+GS+TD ++E +LGFLRLFR RLI++L +GY+IRILLWTF+QSFKALP+VCLLIG+LFFIY I+GMQ+FGNI LN T+IN++NNF++F ++LLFRCATGE W IM+ GK CA G ++ + + CGS+ SY YF +F+ +++FLMLNLFVAVIMDNFDYLTRD SILGPHHLDE++R+WA++DP TG +++ +MY+M++ + PPVGFGK CPYR A LIRMNM + V+F +TLFALIRESL IKM V E+D D+ELR+ + K+WP K+N +D +P N LTVGKIYAG+LI++N+R
Sbjct: 918 KPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKNRKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQGEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMK---------YYNNPLFYEE---------ILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGH-----FVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCA----RAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTVHFTTTLFALIRESLSIKMRPVEEMDEADEELRLTLKKIWPLKAKKNMVDLVVPPNHELCFQKLTVGKIYAGLLILENYR 1673 HSP 2 Score: 541.191 bits (1393), Expect = 4.832e-160 Identity = 358/691 (51.81%), Postives = 460/691 (66.57%), Query Frame = 2
Query: 131 YLARQTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV----------NLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTS-------CNA-GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQK-TKEWLERKMRQDE------YICXXXXXXXXXXXXXXXIENWI-------------KYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
++ + TLS + ++ P SLFIF+E+NIIRR+ K +IE FEY ++LTII NCV L++E HLP+ D+ + + EP F+GIF E +LK+IA GF + K +YL+S WNI+DFIVV G++T+ N D+VDLRT RA+RVLRP KLVSG+PSLQVVL SI+ AMAPLLQIGLLVLFA++IFAIIGLE Y G FH+ C+ + + P +N + C+ G TC W GP +G+ +FDNIG+AM+TVFQCITMEGWT V+Y TN + S +N YF+ LIVLGSFFMLNLVLGVLSGEFAKERERVE RR FLKLR Q+ E L+GY+ W+ TAEE+I E++ EEK +M+ R++ + L++ +Q + + + ++ +E+ K+I ++R ++ +KTQ FYW VITLV LNT CVA EHYGQP++ FL YAE+VFL IF+ E+ +K+F G R YF SKFN FD +V+ S E I+ + SFG S +R +RLLR+ KLT YW SLRNLV SL++SMRSIISLL LL LFI I LLGMQLFGG FNF P +FDTFP A+ITVF ILTGE+WNEVMY I SQ + YS YF+++V+FGNYTLLNVFLAIAVD
Sbjct: 160 FVRKTTLSSNAPVKEKGPS-SLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEI--ENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEGGTVEDEFAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVY-GGSFGISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQGGIYSG---GWPYSIYFIVLVLFGNYTLLNVFLAIAVD 843 HSP 3 Score: 109.768 bits (273), Expect = 1.713e-23 Identity = 100/342 (29.24%), Postives = 158/342 (46.20%), Query Frame = 2
Query: 2207 KIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAE-----GIIVRKKILPY---------SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLR--NRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFF-YSKNLNT---------IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFF-YCNDLSKRIKETCKGYFYTFN----------DQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL---KNSIYATYS 3106
++ D+ D L M + +Q S + E E G VRK L SSLFIF N IRR + F+ FI++ I + + L+ E + N + + LE + F +F LE +LKVI G LH GSY+R WNIMD IVV+ V + +S T +++LR +RVLRPLK ++ +P L+ V ++I+ ++ + I ++ + IF +I ++ +SG F C + I+ + N D G ++ NY ++DNI +MIT+F T EGW V+ +S+ +TY+
Sbjct: 114 RVVDVSDHKTCALLMTRMKEASRQLPSPSQLAAEEARREQKAESGTFVRKTTLSSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYN 455 HSP 4 Score: 97.8265 bits (242), Expect = 6.053e-20 Identity = 78/271 (28.78%), Postives = 126/271 (46.49%), Query Frame = 2
Query: 161 FVGQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGD-RTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDV 934
F G KP P S+F S N R ++ ++FE +V+ I + VSLA E + + + R ++ M + F G+F E +LK+I G ++ +Y + WNILD IVVT L +++ R +RVLRP K + +P L+ V ++ ++ + I ++ IFA+I ++++ G F + TD F T C+ Q N + + + PF N+DN AMLT+F T EGW +
Sbjct: 914 FGGPKPMVPYSSMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYM---DYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFF---FCTDKNRKFANT---CHGQFFVYDNQNDPPRVEQREWRLRPF----NYDNTINAMLTLFVVTTGEGWPGI 1171 HSP 5 Score: 73.9442 bits (180), Expect = 1.133e-12 Identity = 69/252 (27.38%), Postives = 127/252 (50.40%), Query Frame = 2
Query: 1376 HFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPK-QSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQ-MKTSNSRDF---------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
+ + PF + ++T++ NT + +++Y P F + L ++F E +KI FG+R+YFR +N FDF+ V S+ +A+ S GF L L L Y ++R L+++ + S +++ + LL+G+ FI ++GMQ+FG + E + NF +F NA+I +F TGE W ++M ++ + + S + S + +YF V ++ +LN+F+A+ +D
Sbjct: 1267 RLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEFGGHFVSLGFLRLFRAARLIRLLQQGY--TIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHN-NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVIMD 1515 HSP 6 Score: 73.1738 bits (178), Expect = 2.094e-12 Identity = 73/310 (23.55%), Postives = 129/310 (41.61%), Query Frame = 2
Query: 188 RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVL------------------YQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
RSLF+ + N + L+ FEY ++ I N + L ++ + + F + +FT E+ILKI+A G Y + WN DF+ V +G IT L + LR FRA R++R L+ +++++L + +++ L + LL+ I+AI+G++V+ I+ N + + + N NF + A++ +F+C T EGW D++ ++ + S + YF + L SF MLNL + V+
Sbjct: 1250 RSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYY---NNPLFYEEILRLFNTALTAVFTVESILKILAFGV----RNYFRDGWNRFDFVTV-VGSITDALVTEFGGHFVSLGFLRLFRAARLIR---LLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEIN--------------RHN---------------------NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNVSYAYFTSFVFLSSFLMLNLFVAVI 1513 HSP 7 Score: 71.2478 bits (173), Expect = 7.588e-12 Identity = 62/223 (27.80%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 3527 LKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIM-IDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
LKY F IF E +LK+ A + +F +N FD V ++GS +V +E+ + +R R++R+ K+ S+R L+ + + S +++ + L+ L I+ ++GMQ+FG N T ++ +F F + L+ +F+ TGE W+ +M + ES G + +GG S YFI +L + +LN+F+A+ +DN
Sbjct: 647 LKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSF-----GISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGG-RFNFPT-MHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQ-----GGIYSGGWPYS------------IYFIVLVLFGNYTLLNVFLAIAVDNL 845 HSP 8 Score: 70.0922 bits (170), Expect = 1.947e-11 Identity = 83/342 (24.27%), Postives = 144/342 (42.11%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDV--FLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF------------GNIE------------------LNDDTQ--------INQN---NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
P+S ++ A N + ++ PFEY I++ I + + L ++ H LP +++ K L E L+ F IF EC+LK+IA+ F L + + WNI DF+ V+ + + F ++ + +L LR RV+R +K++ S++++L + + + L + LL+ I+ IIG++ + G IE N D + I N +F N ++ +F+C T E W +M T D L GS +++ YFI I++ +F MLNL + V+ F
Sbjct: 177 PSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQH--LP----KNDKKALSEW---------LERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYT--------------------NDSL-GSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEF 482
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0474241) HSP 1 Score: 1327.77 bits (3435), Expect = 0.000e+0 Identity = 818/1534 (53.32%), Postives = 1055/1534 (68.77%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN---------AGFTCK-------PYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN-DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQND-FCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELND-DTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P + +S T CN F C W+GP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L +++ D ++ + K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H Q D + SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E S N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + I ++NNF++F+ G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WR+
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP-----NKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG----MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENGDGAVAPSKSKGKK---KEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHN-------------------QGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSI-----NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWP-LQAKKMLNLLVPPSDQLNKGK--LSVGKIYAGFLILESWRS 1493 HSP 2 Score: 155.221 bits (391), Expect = 3.152e-37 Identity = 163/679 (24.01%), Postives = 300/679 (44.18%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ-SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG------KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD A Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG N + G NF +F ++ +F TGE W +M ++ + K S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG---NIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1335 HSP 3 Score: 101.293 bits (251), Expect = 7.829e-21 Identity = 83/284 (29.23%), Postives = 135/284 (47.54%), Query Frame = 2
Query: 2291 SPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIK--------------ETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
P ENPP G +SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +GLVLH SY+R+ WNIMD VV+ + + +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D +K +K E G F N K ++DNI +M+T+F T EGW +L
Sbjct: 3 GPKKEENPPGGGP---------TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAIL 277 HSP 4 Score: 67.3958 bits (163), Expect = 1.599e-10 Identity = 77/346 (22.25%), Postives = 134/346 (38.73%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFD-FVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI----------------ELNDDTQINQNN------------------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
PTS ++ N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A L + ++ WNI D FV V G +T + + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + E +T N +N +F N +L +F+C T E W I+ T + GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 15 PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------GGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMT-------QYPQIGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT---------------------NDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEF 316
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0474240) HSP 1 Score: 1325.84 bits (3430), Expect = 0.000e+0 Identity = 818/1534 (53.32%), Postives = 1055/1534 (68.77%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN---------AGFTCK-------PYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN-DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQND-FCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELND-DTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P + +S T CN F C W+GP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L +++ D ++ + K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H Q D + SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E S N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + I ++NNF++F+ G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WR+
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP-----NKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG----MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENGDGAVAPSKSKGKK---KEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHN-------------------QGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSI-----NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWP-LQAKKMLNLLVPPSDQLNKGK--LSVGKIYAGFLILESWRS 1493 HSP 2 Score: 155.992 bits (393), Expect = 1.836e-37 Identity = 163/679 (24.01%), Postives = 300/679 (44.18%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ-SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG------KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD A Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG N + G NF +F ++ +F TGE W +M ++ + K S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG---NIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1335 HSP 3 Score: 101.293 bits (251), Expect = 6.715e-21 Identity = 83/284 (29.23%), Postives = 135/284 (47.54%), Query Frame = 2
Query: 2291 SPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIK--------------ETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
P ENPP G +SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +GLVLH SY+R+ WNIMD VV+ + + +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D +K +K E G F N K ++DNI +M+T+F T EGW +L
Sbjct: 3 GPKKEENPPGGGP---------TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAIL 277 HSP 4 Score: 67.0106 bits (162), Expect = 1.734e-10 Identity = 77/346 (22.25%), Postives = 134/346 (38.73%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFD-FVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI----------------ELNDDTQINQNN------------------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
PTS ++ N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A L + ++ WNI D FV V G +T + + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + E +T N +N +F N +L +F+C T E W I+ T + GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 15 PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------GGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMT-------QYPQIGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT---------------------NDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEF 316
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0474239) HSP 1 Score: 1325.07 bits (3428), Expect = 0.000e+0 Identity = 818/1534 (53.32%), Postives = 1055/1534 (68.77%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN---------AGFTCK-------PYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN-DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQND-FCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELND-DTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P + +S T CN F C W+GP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L +++ D ++ + K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H Q D + SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E S N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + I ++NNF++F+ G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WR+
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP-----NKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG----MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENGDGAVAPSKSKGKK---KEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHN-------------------QGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSI-----NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWP-LQAKKMLNLLVPPSDQLNKGK--LSVGKIYAGFLILESWRS 1493 HSP 2 Score: 155.606 bits (392), Expect = 2.239e-37 Identity = 163/679 (24.01%), Postives = 300/679 (44.18%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ-SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG------KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD A Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG N + G NF +F ++ +F TGE W +M ++ + K S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG---NIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1335 HSP 3 Score: 101.293 bits (251), Expect = 6.930e-21 Identity = 83/284 (29.23%), Postives = 135/284 (47.54%), Query Frame = 2
Query: 2291 SPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIK--------------ETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
P ENPP G +SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +GLVLH SY+R+ WNIMD VV+ + + +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D +K +K E G F N K ++DNI +M+T+F T EGW +L
Sbjct: 3 GPKKEENPPGGGP---------TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAIL 277 HSP 4 Score: 67.0106 bits (162), Expect = 1.949e-10 Identity = 79/346 (22.83%), Postives = 135/346 (39.02%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFD-FVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI----------------ELNDDTQINQNN------------------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
PTS ++ N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A L + ++ WNI D FV V G +T + + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + E +T N +N +F N +L +F+C T E W I+ T D L GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 15 PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------GGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMT-------QYPQIGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT--------------------NDAL-GSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEF 316
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0307317) HSP 1 Score: 1325.07 bits (3428), Expect = 0.000e+0 Identity = 818/1534 (53.32%), Postives = 1055/1534 (68.77%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN---------AGFTCK-------PYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN-DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQND-FCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELND-DTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P + +S T CN F C W+GP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L +++ D ++ + K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H Q D + SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E S N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + I ++NNF++F+ G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WR+
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP-----NKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG----MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENGDGAVAPSKSKGKK---KEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHN-------------------QGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSI-----NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWP-LQAKKMLNLLVPPSDQLNKGK--LSVGKIYAGFLILESWRS 1493 HSP 2 Score: 155.606 bits (392), Expect = 2.274e-37 Identity = 163/679 (24.01%), Postives = 300/679 (44.18%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ-SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG------KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD A Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG N + G NF +F ++ +F TGE W +M ++ + K S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG---NIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1335 HSP 3 Score: 101.293 bits (251), Expect = 7.346e-21 Identity = 83/284 (29.23%), Postives = 135/284 (47.54%), Query Frame = 2
Query: 2291 SPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIK--------------ETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
P ENPP G +SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +GLVLH SY+R+ WNIMD VV+ + + +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D +K +K E G F N K ++DNI +M+T+F T EGW +L
Sbjct: 3 GPKKEENPPGGGP---------TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAIL 277 HSP 4 Score: 67.0106 bits (162), Expect = 1.948e-10 Identity = 77/346 (22.25%), Postives = 134/346 (38.73%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFD-FVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI----------------ELNDDTQINQNN------------------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
PTS ++ N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A L + ++ WNI D FV V G +T + + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + E +T N +N +F N +L +F+C T E W I+ T + GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 15 PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------GGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMT-------QYPQIGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT---------------------NDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEF 316
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Fly
Match: cac (gene:FBgn0263111 transcript:FBtr0332391) HSP 1 Score: 1323.92 bits (3425), Expect = 0.000e+0 Identity = 811/1533 (52.90%), Postives = 1052/1533 (68.62%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN---------AGFTCK-------PYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN-DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQND-FCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P + +S T CN F C W+GP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEF+ ER RVE+R F K R ++ + Y++W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L +++ D ++ + K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H Q D + SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E + ++ N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI + DTQ+N++NNF++F G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WR+
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP-----NKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFSNERNRVERRMEFQKCRFRAMFQTAMVSYLDWITQAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG----MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENGDGAVAPSKSKGKK---KEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHN-------------------QGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDSNSI---NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRYDPDTQLNRHNNFQSFSGGIMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWP-LQAKKMLNLLVPPSDQLNKGK--LSVGKIYAGFLILESWRS 1494 HSP 2 Score: 157.532 bits (397), Expect = 5.264e-38 Identity = 163/678 (24.04%), Postives = 301/678 (44.40%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ---SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD A Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG D + + + NF +F I+ +F TGE W +M ++ + K S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIRYDPDTQLNRHN-NFQSFSGGIMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1336 HSP 3 Score: 101.293 bits (251), Expect = 7.625e-21 Identity = 83/284 (29.23%), Postives = 135/284 (47.54%), Query Frame = 2
Query: 2291 SPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIK--------------ETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
P ENPP G +SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +GLVLH SY+R+ WNIMD VV+ + + +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D +K +K E G F N K ++DNI +M+T+F T EGW +L
Sbjct: 3 GPKKEENPPGGGP---------TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAIL 277 HSP 4 Score: 67.0106 bits (162), Expect = 1.735e-10 Identity = 77/347 (22.19%), Postives = 134/347 (38.62%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFD-FVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI----------------ELNDDTQINQNN------------------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFD 4195
PTS ++ N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A L + ++ WNI D FV V G +T + + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + E +T N +N +F N +L +F+C T E W I+ T + GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 15 PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------GGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMT-------QYPQIGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT---------------------NDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFS 317
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Zebrafish
Match: cacna1da (calcium channel, voltage-dependent, L type, alpha 1D subunit, a [Source:ZFIN;Acc:ZDB-GENE-030616-135]) HSP 1 Score: 1021.92 bits (2641), Expect = 0.000e+0 Identity = 665/1560 (42.63%), Postives = 944/1560 (60.51%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV------------------------DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN-AGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEEL------IRFENKQNAEEKKELMKNRQKTKEWLER---------------------KMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAI---FQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGI---IVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ V P+ D + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H +C+ + L D +C G CK W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ E+K+N LM+ ++K W + K ++ C + + S W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I F+I++P G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S+S MI YF+++ I GNY LLNVFLAIAVDNLA+A L+ + K D D I +E ++ + E V P I + ++KI P S+ FIF N +R CH + N F I++ I SS +LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ CND +K E CKG + + + Q +KR W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L++I L ++ + QS ++ + + LN+ FT +FT E +LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ + E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 54 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYVPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKEGGEKEEVGENLSAHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHASCYFQGTDILEDEPAPCAVNGHGRTCPINGTLCKEGWQGPNGGITNFDNFMFAMLTVFQCITMEGWTDVLYWMNDAMGLELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEEEESKRNRVTLASLMEKKKKGFGWFSQSSDTHASMPASETESMNTENEKGEDEKATCCGPTCQKISKSKFS--RRWRRWNRLCRRNCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPMWLTQVQDVANKVLLAMFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVEFEIMSP---LGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSS---GMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNT--------------------------DDTKKPDEIDEIEDEAKAGEEDEKDNAEEDEEEPDVPAGPRPKISELVKKEKITPIPEGSAFFIFSNTNPVRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIILGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYIMYKEGDVNQPIIQKRHWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENMGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLILLNTICLAVQHYG----------------QSELFNYVMDI--LNMVFTAVFTVEMVLKLIAFKPRHYFTDAWNTFDALIVVGSVVDIAITEVNNTEDSARI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDHTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACMPGKLCDPESDYNPG-----EEMTCGSSFAIIYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1549 HSP 2 Score: 107.457 bits (267), Expect = 1.387e-22 Identity = 76/265 (28.68%), Postives = 131/265 (49.43%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N +R +C LI F L+++ I+ + SLA E + I+ + + F IFT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S Y + + N K +W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 802 PIPEGSAFFIFSNTNPVRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNII--LGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYIMYKEGDV--NQPIIQKRHWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1056 HSP 3 Score: 72.4034 bits (176), Expect = 5.636e-12 Identity = 89/358 (24.86%), Postives = 145/358 (40.50%), Query Frame = 2
Query: 3293 AINTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMK-------------SEESTTEIPNLGF----LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-----------GNIELNDD---TQINQNN----------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ NT+P N N + LV PF+ I+I I + + L + + PE SN ST +D L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + S P GF LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G L D+ +N + NF NFM +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 49 SANTRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YVPFPED--DSN-------STNHD----LETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKEGGEKEEVGENLSAHGHGGKPG-GFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHASCYFQGTDILEDEPAPCAVNGHGRTCPINGTLCKEGWQGPNGGITNFDNFMFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGLELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 369
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Zebrafish
Match: cacna1da (calcium channel, voltage-dependent, L type, alpha 1D subunit, a [Source:ZFIN;Acc:ZDB-GENE-030616-135]) HSP 1 Score: 1011.52 bits (2614), Expect = 0.000e+0 Identity = 658/1543 (42.64%), Postives = 931/1543 (60.34%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV------------------------DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN-AGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAI---FQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGI---IVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMK-------SEESTTEIP---NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ V P+ D + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H +C+ + L D +C G CK W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ ++ E K+ +T+ + ++ W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I F+I++P G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S+S MI YF+++ I GNY LLNVFLAIAVDNLA+A L+ + K D D I +E ++ + E V P I + ++KI P S+ FIF N +R CH + N F I++ I SS +LAAEDP+ S RN +L + D FTS+F E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ CND +K E CKG + + + Q +KR W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L++I L ++ + QS ++ + + LN+ FT +FT E +LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ E TE ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 54 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYVPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKEGGEKEEVGENLSAHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHASCYFQGTDILEDEPAPCAVNGHGRTCPINGTLCKEGWQGPNGGITNFDNFMFAMLTVFQCITMEGWTDVLYWMNDAMGLELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEEEESKRNPSMPASETESMNTENEKGEDEKATCCGPTCQKISKSKFSRRWRRWNRLCRRNCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPMWLTQVQDVANKVLLAMFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVEFEIMSP---LGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSS---GMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNT--------------------------DDTKKPDEIDEIEDEAKAGEEDEKDNAEEDEEEPDVPAGPRPKISELVKKEKITPIPEGSAFFIFSNTNPVRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIVLGYADYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYIMYKEGDVNQPIIQKRHWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENMGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLILLNTICLAVQHYG----------------QSELFNYVMDI--LNMVFTAVFTVEMVLKLIAFKPRHYFTDAWNTFDALIVVGSVVDIAITEVNPTEAPQVDESGNTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDHTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACMPGKLCDPESDYNPG-----EEMTCGSSFAIIYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1535 HSP 2 Score: 107.457 bits (267), Expect = 1.294e-22 Identity = 77/265 (29.06%), Postives = 132/265 (49.81%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N +R +C LI F L+++ I+ + SLA E + IV A + +F +FT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S Y + + N K +W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 777 PIPEGSAFFIFSNTNPVRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIVLGYA--DYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYIMYKEGDV--NQPIIQKRHWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1031 HSP 3 Score: 72.4034 bits (176), Expect = 5.773e-12 Identity = 89/358 (24.86%), Postives = 145/358 (40.50%), Query Frame = 2
Query: 3293 AINTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMK-------------SEESTTEIPNLGF----LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-----------GNIELNDD---TQINQNN----------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ NT+P N N + LV PF+ I+I I + + L + + PE SN ST +D L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + S P GF LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G L D+ +N + NF NFM +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 49 SANTRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YVPFPED--DSN-------STNHD----LETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKEGGEKEEVGENLSAHGHGGKPG-GFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHASCYFQGTDILEDEPAPCAVNGHGRTCPINGTLCKEGWQGPNGGITNFDNFMFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGLELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 369
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Zebrafish
Match: cacna1fb (calcium channel, voltage-dependent, L type, alpha 1F subunit [Source:ZFIN;Acc:ZDB-GENE-031104-1]) HSP 1 Score: 961.059 bits (2483), Expect = 0.000e+0 Identity = 655/1567 (41.80%), Postives = 946/1567 (60.37%), Query Frame = 2
Query: 116 KDTIKYLARQTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT----------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCW--KTDS-------PFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTK-EWLE----------RKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAK---IQDIDDSIIEELNMYDKPKDKKQPIKESPVIVE----NPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFF-YSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYF--YTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
K +++ +Q + V + P R+L+ NN IRR+ L+E + F+ ++L I ANCV+L + P+ D + +E +FL IFT E LKI+A G ++ ++Y+++ WN+LDF++V +GL +V L+ T +D++ RA RVLRP +LVSGVPSLQ+VL SI+KAM PLL I LLVLF ++I+AIIGLE++ G H TC+ TD+ P F + CY G C+ W+GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GYM+W+ AE++ F+ N + ++++ K W + D + D + A+ W + I R + +K+ FYW V+ LV LNTA A EHY QP++L A V LS+F E+ +K++ G++ YF + FN FD VV ++E + + G S LRC+RLLR+ K+TR+W++L NLV SLL+SM+SI SLLLLL LF+ I LLGMQLFGG FNF++ + + FD+FP A++T F ILTGE+WN VMY+GI M MI YF+++ I GNY LLNVFLAIAVDNLA + + G + K + ++D +I +++Y P++E + + PP E KILP S+ F N IR CH + + F I++ I SS++LAAEDP+ +S RN +L + D AFTS+F +E++LK+ G LH GS+ R+ +N++D++VV +++ SFF +S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K E CKG F Y D ++RIW + FN+DN++ M+ LFTV+T EGWP +L +I A N GPI NY+ EI+IF+IV+ I+ FF +NIFV +I+TF+EQGE++ + ELDKN+ +C+++A+ +P Y+P KN V YK W ++ S FEY++ ++I L+++TL ++ + + +KF F + LN+ FT +FT E I+K++A + + +F D WN FD + V+GS+ D+ ++E S E ++ + ++ F RLFRVMRL+K+L +G IR LLWTF++S +ALP+V LLI ++FFIY +IGMQ FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ + GKRC + G ++ CGS+ + YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L+ V FN+TLFAL+R +L+IK + +N +ELRI + K+W K +D +P A +TVGK YA LI +R F K
Sbjct: 41 KKKSQHVKKQVQGSNQVQRAP---RALYCLKLNNPIRRAALHLVEWKPFDIFILLAIFANCVALGVSKPFPEDDSNATNHDLEQVEYVFLIIFTIETFLKILAYGLVMHPSSYIRNGWNLLDFVIVIVGLFSVVLETVTHKSGETTSHTPGKPGGLDVKALRAFRVLRPLRLVSGVPSLQIVLNSIMKAMVPLLHISLLVLFVIIIYAIIGLELFIGRMHRTCFFIGTDNYADDDPLPCAFAGHGRQCYVN-------GSECRGKWEGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMNDAIGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLCGYMDWITQAEDIDEFDEDGNRRVTLRDLADKKRGKFGWFSHSNETHGTANKNEPHDPFTPSTALYIDPLNIVCRALRRWNRCI---RRNCRTAVKSVTFYWLVLILVFLNTALSASEHYNQPEWLTDVQDIANKVLLSLFTVEMLLKMYSLGLQVYFVAFFNRFDCFVVCGGILETVLVEMEIMPPLGISVLRCVRLLRIFKVTRHWTALSNLVASLLNSMKSIASLLLLLFLFLIIFALLGMQLFGGKFNFDETQTKRSTFDSFPQALLTCFQILTGEDWNVVMYDGI---MAYGGPVFPGMIVCLYFVILFICGNYILLNVFLAIAVDNLAGGDGDNKKNDPSGVSRG--------------LGCQLKGTVLIELDCDLINSISLYFFLS--IFPLEEGKIQLNVADFAPPKE------KILPIPEGSAFFCLSKTNPIRVGCHTLIHHHIFTNLILVFIILSSISLAAEDPIRAHSFRNNVLGYADYAFTSIFTVEILLKMTVHGAFLHEGSFCRNWFNLLDLLVVSVSLVSFFLHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKNTPEQCKGTFVVYKDGDVSHPMVRERIWLNSDFNFDNVLMGMMALFTVSTFEGWPALLYKAIDANGENHGPIYNYRVEISIFFIVYIIIIAFFMMNIFVGFVIITFREQGEAEFKNCELDKNQRQCVEYALKAQPLKLYIP--KNPVQYKFWSIINSTGFEYVMFVLILLNTVTLAVQHYDQ---------SKF---------FSQVMDILNMVFTGLFTVEMIIKLMALRLRHYFVDAWNSFDALIVVGSVVDIVVTEFSSSEDSSRV-SITFFRLFRVMRLVKLLSKGEGIRTLLWTFVKSLQALPYVALLIAMIFFIYAVIGMQTFGKIAMQDHTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGKRCDAESDFEPG-----EEFSCGSNIAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNADGTVTFNATLFALVRTALKIKTDGNPDQEN--EELRIIIKKIWKR-TKPKVLDEVIP-PPAEEEVTVGKFYATFLIQDYFRKFRK 1539
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Zebrafish
Match: cacna1fb (calcium channel, voltage-dependent, L type, alpha 1F subunit [Source:ZFIN;Acc:ZDB-GENE-031104-1]) HSP 1 Score: 938.717 bits (2425), Expect = 0.000e+0 Identity = 641/1566 (40.93%), Postives = 925/1566 (59.07%), Query Frame = 2
Query: 116 KDTIKYLARQTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT----------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCW--KTDS-------PFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTK-EWLERKMR----------QDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAK---IQDIDDSIIEELNMYDKPKDKKQPIKESPVIVE----NPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYF--YTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
K +++ +Q + V + P R+L+ NN IRR+ L+E + F+ ++L I ANCV+L + P+ D + +E +FL IFT E LKI+A G ++ ++Y+++ WN+LDF++V +GL +V L+ T +D++ RA RVLRP +LVSGVPSLQ+VL SI+KAM PLL I LLVLF ++I+AIIGLE++ G H TC+ TD+ P F + CY G C+ W+GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GYM+W+ AE++ F+ N + ++++ K W D + D + A+ W + I R + +K+ FYW V+ LV LNTA A EHY QP++L A V LS+F E+ +K++ G++ YF + FN FD VV ++E + + G S LRC+RLLR+ K+TR+W++L NLV SLL+SM+SI SLLLLL LF+ I LLGMQLFGG FNF++ + + FD+FP A++T F ILTGE+WN VMY+GI M MI YF+++ I GNY LLNVFLAIAVD + ++ + + G + K + ++D +I + +Y P++E + + PP E KILP S+ F N IR CH + + F I++ I SS++LAAEDP+ +S RN +L + D AFTS+F +E++LKV+ + + D + S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K E CKG F Y D ++RIW + FN+DN++ M+ LFTV+T EGWP +L +I A N GPI NY+ EI+IF+IV+ I+ FF +NIFV +I+TF+EQGE++ + ELDKN+ +C+++A+ +P Y+P KN V YK W ++ S FEY++ ++I L+++TL ++ + + +KF F + LN+ FT +FT E I+K++A + + +F D WN FD + V+GS+ D+ ++E S E ++ + ++ F RLFRVMRL+K+L +G IR LLWTF++S +ALP+V LLI ++FFIY +IGMQ FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ + GKRC + G ++ CGS+ + YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L+ V FN+TLFAL+R +L+IK + +N +ELRI + K+W K +D +P A +TVGK YA LI +R F K
Sbjct: 41 KKKSQHVKKQVQGSNQVQRAP---RALYCLKLNNPIRRAALHLVEWKPFDIFILLAIFANCVALGVSKPFPEDDSNATNHDLEQVEYVFLIIFTIETFLKILAYGLVMHPSSYIRNGWNLLDFVIVIVGLFSVVLETVTHKSGETTSHTPGKPGGLDVKALRAFRVLRPLRLVSGVPSLQIVLNSIMKAMVPLLHISLLVLFVIIIYAIIGLELFIGRMHRTCFYIGTDNYADDDPLPCAFAGHGRQCYVN-------GSECRGKWEGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMNDAIGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLCGYMDWITQAEDIDEFDEDGNRRVTLRDLADKKRGKFGWFSHSNETHGTANQNEPHDPFTPSTALYIDPLNIVCRALRRWNRCI---RRNCRTAVKSVTFYWLVLILVFLNTALSASEHYNQPEWLTDVQDIANKVLLSLFTVEMLLKMYSLGLQVYFVAFFNRFDCFVVCGGILETVLVEMEIMPPLGISVLRCVRLLRIFKVTRHWTALSNLVASLLNSMKSIASLLLLLFLFLIIFALLGMQLFGGKFNFDETQTKRSTFDSFPQALLTCFQILTGEDWNVVMYDGI---MAYGGPVFPGMIVCLYFVILFICGNYILLNVFLAIAVD--------------NLAGGDGDNKKNDPSGVSRGLGCQLKGTVLIELDCDLINSIPLYFFL--SIFPLEEGKIQLNVADFAPPKE------KILPIPEGSAFFCLSKTNPIRVGCHTLIHHHIFTNLILVFIILSSISLAAEDPIRAHSFRNNVLGYADYAFTSIFTVEILLKVLRQKNIFAVSIFSSD-----------LFFSISLSSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKNTPEQCKGTFVVYKDGDVSHPMVRERIWLNSDFNFDNVLMGMMALFTVSTFEGWPALLYKAIDANGENHGPIYNYRVEISIFFIVYIIIIAFFMMNIFVGFVIITFREQGEAEFKNCELDKNQRQCVEYALKAQPLKLYIP--KNPVQYKFWSIINSTGFEYVMFVLILLNTVTLAVQHYDQ---------SKF---------FSQVMDILNMVFTGLFTVEMIIKLMALRLRHYFVDAWNSFDALIVVGSVVDIVVTEFSSSEDSSRV-SITFFRLFRVMRLVKLLSKGEGIRTLLWTFVKSLQALPYVALLIAMIFFIYAVIGMQTFGKIAMQDHTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGKRCDAESDFEPG-----EEFSCGSNIAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNADGTVTFNATLFALVRTALKIKTDGNPDQEN--EELRIIIKKIWKR-TKPKVLDEVIP-PPAEEEVTVGKFYATFLIQDYFRKFRK 1527
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Zebrafish
Match: cacna1fb (calcium channel, voltage-dependent, L type, alpha 1F subunit [Source:ZFIN;Acc:ZDB-GENE-031104-1]) HSP 1 Score: 912.138 bits (2356), Expect = 0.000e+0 Identity = 636/1566 (40.61%), Postives = 914/1566 (58.37%), Query Frame = 2
Query: 116 KDTIKYLARQTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT----------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCW--KTDS-------PFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTK-EWLE----------RKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAK---IQDIDDSIIEELNMYDKPKDKKQPIKESPVIVE----NPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYF--YTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
K +++ +Q + V + P R+L+ NN IRR+ L+E + F+ ++L I ANCV+L + P+ D + +E +FL IFT E LKI+A G ++ ++Y+++ WN+LDF++V +GL +V L+ T +D++ RA RVLRP +LVSGVPSLQ+VL SI+KAM PLL I LLVLF ++I+AIIGLE++ G H TC+ TD+ P F + CY G C+ W+GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GYM+W+ AE++ F+ N + ++++ K W + D + D + A+ W + I R + +K+ FYW V+ LV LNTA A EHY QP++L A V LS+F E+ +K++ G++ YF + FN FD VV ++E + + G S LRC+RLLR+ K+TR+W++L NLV SLL+SM+SI SLLLLL LF+ I LLGMQLFGG FNF++ + + FD+FP A++T F ILTGE+WN VMY+GI M MI YF+++ I GNY LLNVFLAIAVDNLA + + G + K + ++D +I +++Y P++E + + PP E KILP S+ F N IR CH + + F I++ I SS++LAAEDP+ +S RN + L F++ + P D + S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K E CKG F Y D ++RIW + FN+DN++ M+ LFTV+T EGWP +L +I A N GPI NY+ EI+IF+IV+ I+ FF +NIFV +I+TF+EQGE++ + ELDKN+ +C+++A+ +P Y+P KN V YK W ++ S FEY++ ++I L+++TL ++ + + +KF F + LN+ FT +FT E I+K++A + + +F D WN FD + V+GS+ D+ ++E S E ++ + ++ F RLFRVMRL+K+L +G IR LLWTF++S +ALP+V LLI ++FFIY +IGMQ FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ + GKRC + G ++ CGS+ + YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L+ V FN+TLFAL+R +L+IK + +N +ELRI + K+W K +D +P A +TVGK YA LI +R F K
Sbjct: 41 KKKSQHVKKQVQGSNQVQRAP---RALYCLKLNNPIRRAALHLVEWKPFDIFILLAIFANCVALGVSKPFPEDDSNATNHDLEQVEYVFLIIFTIETFLKILAYGLVMHPSSYIRNGWNLLDFVIVIVGLFSVVLETVTHKSGETTSHTPGKPGGLDVKALRAFRVLRPLRLVSGVPSLQIVLNSIMKAMVPLLHISLLVLFVIIIYAIIGLELFIGRMHRTCFFIGTDNYADDDPLPCAFAGHGRQCYVN-------GSECRGKWEGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMNDAIGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLCGYMDWITQAEDIDEFDEDGNRRVTLRDLADKKRGKFGWFSHSNETHGTANKNEPHDPFTPSTALYIDPLNIVCRALRRWNRCI---RRNCRTAVKSVTFYWLVLILVFLNTALSASEHYNQPEWLTDVQDIANKVLLSLFTVEMLLKMYSLGLQVYFVAFFNRFDCFVVCGGILETVLVEMEIMPPLGISVLRCVRLLRIFKVTRHWTALSNLVASLLNSMKSIASLLLLLFLFLIIFALLGMQLFGGKFNFDETQTKRSTFDSFPQALLTCFQILTGEDWNVVMYDGI---MAYGGPVFPGMIVCLYFVILFICGNYILLNVFLAIAVDNLAGGDGDNKKNDPSGVSRG--------------LGCQLKGTVLIELDCDLINSISLYFFLS--IFPLEEGKIQLNVADFAPPKE------KILPIPEGSAFFCLSKTNPIRVGCHTLIHHHIFTNLILVFIILSSISLAAEDPIRAHSFRNNI----SLCFSAD--------------LFKPTCTKSD-----------LFFSISLSSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKNTPEQCKGTFVVYKDGDVSHPMVRERIWLNSDFNFDNVLMGMMALFTVSTFEGWPALLYKAIDANGENHGPIYNYRVEISIFFIVYIIIIAFFMMNIFVGFVIITFREQGEAEFKNCELDKNQRQCVEYALKAQPLKLYIP--KNPVQYKFWSIINSTGFEYVMFVLILLNTVTLAVQHYDQ---------SKF---------FSQVMDILNMVFTGLFTVEMIIKLMALRLRHYFVDAWNSFDALIVVGSVVDIVVTEFSSSEDSSRV-SITFFRLFRVMRLVKLLSKGEGIRTLLWTFVKSLQALPYVALLIAMIFFIYAVIGMQTFGKIAMQDHTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGKRCDAESDFEPG-----EEFSCGSNIAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNADGTVTFNATLFALVRTALKIKTDGNPDQEN--EELRIIIKKIWKR-TKPKVLDEVIP-PPAEEEVTVGKFYATFLIQDYFRKFRK 1509
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Xenopus
Match: frrs1l (ferric-chelate reductase 1-like [Source:Xenbase;Acc:XB-GENE-6257538]) HSP 1 Score: 1044.26 bits (2699), Expect = 0.000e+0 Identity = 658/1508 (43.63%), Postives = 944/1508 (62.60%), Query Frame = 2
Query: 248 EQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ------------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMK-----------SEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
E R FE +++LTI ANC++LA+ + +P+ D + T + IE IFL IFT EA LKI+A GFI+ +AYL++ WNILDF +V++GL T+ L+ +++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AI+G E++ G H TC+ + + AT + P S T G C+ +W GP G+ +FDN G+AMLTV+QCITMEGWT+VLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R AF KLRE Q + L GY++W+ AE + + + + ++ + + ER C + W + +R + +K++ FYW VI ++L NT +A EH+ QP++L + A V L++F E+ +KI+ G + YF S FN FD VV ++E + N G S LRCIRLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LF+ I LLGMQ+FGG F+FE + + FD FP ++ITVF ILTGE+WN VMYNGI M M YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++ + ++ + + + KIQ ++++ ++ + + I SP P + +++K +P SS FIFGP N R +CH + N F FI++ I SS++LAAEDP+ S RN++L +FD FT++F E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + ++K + NI++V L QF+F I VQLF GKF+ C D +K +E C+GYF D IQ ++RIW + F++DN+++ M++LFT++T EGWP +L +I + + GPI NY+ EIA+F+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+ +A+ +P RY+P N++ YK+W LV S FEYL+ +I L++I+L ++ + + E + S+ LN+AFT IFT E +LK+ A++ K +F DPWN+FDF+ V+GS+ DV LSE+ E T+ I ++ F RLFRVMRL+K+L RG +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +L+LFRCATGE+W I++ GK C + G ++ CG+ F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLF+L+R +L+IK E N +ELR+ + K+W +D +P +TVGK YA LI +++R F K
Sbjct: 54 EYRPFEMIILLTIFANCIALAIYLPMPEDDTNSVNTILEKIEYIFLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQINKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVI--ATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQKLREQQAMDEDLRGYLDWITHAEVMDPDMEQGDEDPAQKNTRCGNGHDQSCER--------CGTG-------------KQWRHWHRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHHNQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGFQSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDTEIRRSTFDNFPQSLITVFQILTGEDWNSVMYNGI---MAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLKPMKK------SAAKKIQHTSKNLLQ-IHAWRNISSLEPEIPISP---RPRPLAELQLKEKAVPMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESFRNQILAYFDYVFTAIFTTEIALKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIPKNQHQ--YKIWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDI------------------LNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDVIWGTFNIVTPGSEDTSRI-SITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKSDFLPG-----EEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVSMNMPLNSDGTVTFNATLFSLVRTALKIKTEGNFEQSN--EELRMIIKKIWKR-TSMKLLDQVIP-PIGDDEVTVGKFYAIFLIQEHFRKFKK 1495 HSP 2 Score: 147.902 bits (372), Expect = 8.156e-35 Identity = 161/681 (23.64%), Postives = 299/681 (43.91%), Query Frame = 2
Query: 176 PNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQAC-----YPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLY---------FVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKK-ELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAI----------FQIINPKQS----FGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFG--GDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDF----------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
P PD S FIF N R C ++ F ++L I+ + +SLA E P +F +A + +F IFTTE LK+ G + K ++ ++ +NILD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I L+ +F+ IG+++++G F++ TD+ T++ C + S + + F +FDN+ M+++F T EGW +LY+ + +Y F++ I+L +FFM+N+ +G + ++ F+ + E K EL KN+++ ++ + YI K H+ + + + + + F + + L++LNT + ++HYGQ F IF E+ +K+ F + YF +NVFDF++V S+++ I F I+ P + R R++RL+KL +R L+++ + S +++ + LL+ + FI ++GMQ+FG + Q NF TFP A++ +F TGE W E++ ++ S DF S Y YF+ + + ++N+F+A+ +D
Sbjct: 737 PMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAED--PIEPESFRNQILAYFDYVFTAIFTTEIALKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSC---TDTT---KMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDF----HFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFV------------------------------------------IVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIP--------------------KNQHQYK--IWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDILNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDVIWGTFNIVTPGSEDTSRISITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKS-DFLPGEEYTCGTSFAY-FYFISFYMLCAFLIINLFVAVIMD 1339 HSP 3 Score: 95.1301 bits (235), Expect = 9.317e-19 Identity = 77/286 (26.92%), Postives = 139/286 (48.60%), Query Frame = 2
Query: 212 NNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGI-EPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV----DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD-----SQWNVLYFVLLIVLGSFFMLNLVLGV 1039
+ ++RR C+ L++ R+F + VIL I+ N +++A E H ++ +TK I + L +FT E +LKI ALGF +Y S++N D VV G++ + L + + + R IR+LR FK+ SL ++ S++ ++ + + LL+ ++IFA++G++++ G F F+ T+ FDN +++TVFQ +T E W V+Y A + +YF++L V G++ +LN+ L +
Sbjct: 415 HRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHH----NQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGF----QSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFD-----------FEDTE-------------------------IRRSTFDNFPQSLITVFQILTGEDWNSVMYNGIMAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAI 656
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Xenopus
Match: frrs1l (ferric-chelate reductase 1-like [Source:Xenbase;Acc:XB-GENE-6257538]) HSP 1 Score: 1041.18 bits (2691), Expect = 0.000e+0 Identity = 663/1509 (43.94%), Postives = 949/1509 (62.89%), Query Frame = 2
Query: 221 IRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ------------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEE---LNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
+RR KG E FE +++LTI ANC++LA+ + +P+ D + T + IE IFL IFT EA LKI+A GFI+ +AYL++ WNILDF +V++GL T+ L+ +++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AI+G E++ G H TC+ + + AT + P S T G C+ +W GP G+ +FDN G+AMLTV+QCITMEGWT+VLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R AF KLRE Q + L GY++W+ T E++ + +Q E E E L +++I S ++ W + +R + +K++ FYW VI ++L NT +A EH+ QP++L + A V L++F E+ +KI+ G + YF S FN FD VV ++E + N G S LRCIRLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LF+ I LLGMQ+FGG F+FE + + FD FP ++ITVF ILTGE+WN VMYNGI M M YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++ + ++ S I+ ++ + +++Y ++++ I SP P + +++K +P SS FIFGP N R +CH + N F FI++ I SS++LAAEDP+ S RN++L +FD FT++F E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + ++K + NI++V L QF+F I VQLF GKF+ C D +K +E C+GYF D IQ ++RIW + F++DN+++ M++LFT++T EGWP +L +I + + GPI NY+ EIA+F+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+ +A+ +P RY+P N++ YK+W LV S FEYL+ +I L++I+L ++ + + E + S+ LN+AFT IFT E +LK+ A++ K +F DPWN+FDF+ V+GS+ DV LSE+ T ++ F RLFRVMRL+K+L RG +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +L+LFRCATGE+W I++ GK C + G ++ CG+ F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLF+L+R +L+IK E N +ELR+ + K+W +D +P +TVGK YA LI +++R F K
Sbjct: 2 LRR--KGCTEP--FEMIILLTIFANCIALAIYLPMPEDDTNSVNTILEKIEYIFLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQINKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVI--ATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQKLREQQAMDEDLRGYLDWI-THAEVMDPDMEQGDEGCSE--------TESLYELEGINKFI--------------SFLKQWRHWHRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHHNQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGFQSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDTEIRRSTFDNFPQSLITVFQILTGEDWNSVMYNGI---MAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLAR--------LQSTPNIEQFLSTVTLKNAFVSIYCDDEEEEPEIPISP---RPRPLAELQLKEKAVPMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESFRNQILAYFDYVFTAIFTTEIALKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIPKNQHQ--YKIWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDI------------------LNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDVSSEDTSRISITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKSDFLPG-----EEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVSMNMPLNSDGTVTFNATLFSLVRTALKIKTEGNFEQSN--EELRMIIKKIWKR-TSMKLLDQVIP-PIGDDEVTVGKFYAIFLIQEHFRKFKK 1438 HSP 2 Score: 148.673 bits (374), Expect = 5.256e-35 Identity = 159/671 (23.70%), Postives = 297/671 (44.26%), Query Frame = 2
Query: 176 PNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQAC-----YPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLY---------FVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKK-ELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQS----FGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFG--GDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDF----------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
P PD S FIF N R C ++ F ++L I+ + +SLA E P +F +A + +F IFTTE LK+ G + K ++ ++ +NILD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I L+ +F+ IG+++++G F++ TD+ T++ C + S + + F +FDN+ M+++F T EGW +LY+ + +Y F++ I+L +FFM+N+ +G + ++ F+ + E K EL KN+++ ++ + YI K H+ + + + + + F + + L++LNT + ++HYGQ F IF E+ +K+ F + YF +NVFDF++V S+++ I I+ + R R++RL+KL +R L+++ + S +++ + LL+ + FI ++GMQ+FG + Q NF TFP A++ +F TGE W E++ ++ S DF S Y YF+ + + ++N+F+A+ +D
Sbjct: 690 PMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAED--PIEPESFRNQILAYFDYVFTAIFTTEIALKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSC---TDTT---KMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDF----HFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFV------------------------------------------IVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIP--------------------KNQHQYK--IWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDILNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDVSSEDTSRISITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKS-DFLPGEEYTCGTSFAY-FYFISFYMLCAFLIINLFVAVIMD 1282 HSP 3 Score: 95.1301 bits (235), Expect = 9.281e-19 Identity = 77/286 (26.92%), Postives = 139/286 (48.60%), Query Frame = 2
Query: 212 NNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIE-PIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV----DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD-----SQWNVLYFVLLIVLGSFFMLNLVLGV 1039
+ ++RR C+ L++ R+F + VIL I+ N +++A E H ++ +TK I + L +FT E +LKI ALGF +Y S++N D VV G++ + L + + + R IR+LR FK+ SL ++ S++ ++ + + LL+ ++IFA++G++++ G F F+ T+ FDN +++TVFQ +T E W V+Y A + +YF++L V G++ +LN+ L +
Sbjct: 366 HRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHH----NQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGF----QSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFD-----------FEDTE-------------------------IRRSTFDNFPQSLITVFQILTGEDWNSVMYNGIMAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAI 607
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Xenopus
Match: frrs1l (ferric-chelate reductase 1-like [Source:Xenbase;Acc:XB-GENE-6257538]) HSP 1 Score: 1033.09 bits (2670), Expect = 0.000e+0 Identity = 656/1500 (43.73%), Postives = 946/1500 (63.07%), Query Frame = 2
Query: 260 FEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ------------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKS-------EESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
FE +++LTI ANC++LA+ + +P+ D + T + IE IFL IFT EA LKI+A GFI+ +AYL++ WNILDF +V++GL T+ L+ +++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AI+G E++ G H TC+ + + AT + P S T G C+ +W GP G+ +FDN G+AMLTV+QCITMEGWT+VLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R AF KLRE Q + L GY++W+ AE + + + + +L + +T+ E + +++I S ++ W + +R + +K++ FYW VI ++L NT +A EH+ QP++L + A V L++F E+ +KI+ G + YF S FN FD VV ++E + N G S LRCIRLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LF+ I LLGMQ+FGG F+FE + + FD FP ++ITVF ILTGE+WN VMYNGI M M YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++ + ++ + F + I+E + ++ + + P+ AE + +++K +P SS FIFGP N R +CH + N F FI++ I SS++LAAEDP+ S RN++L +FD FT++F E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + ++K + NI++V L QF+F I VQLF KF+ C D +K +E C+GYF D IQ ++RIW + F++DN+++ M++LFT++T EGWP +L +I + + GPI NY+ EIA+F+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+ +A+ +P RY+P N++ YK+W LV S FEYL+ +I L++I+L ++ + + E + S+ LN+AFT IFT E +LK+ A++ K +F DPWN+FDF+ V+GS+ DV LSE+ + E T+ I ++ F RLFRVMRL+K+L RG +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +L+LFRCATGE+W I++ GK C + G ++ CG+ F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLF+L+R +L+IK E N +ELR+ + K+W +D +P +TVGK YA LI +++R F K
Sbjct: 5 FEMIILLTIFANCIALAIYLPMPEDDTNSVNTILEKIEYIFLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQINKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVI--ATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQKLREQQAMDEDLRGYLDWITHAE--VMDPDMEQGDGFSQLEEGCSETESLYELE-GINKFI--------------SFLKQWRHWHRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHHNQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGFQSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDTEIRRSTFDNFPQSLITVFQILTGEDWNSVMYNGI---MAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLARGRFTKALTFKKKKLKIKKKKKNIQECDDEEEEPEIPISPRPRPL------AE-LQLKEKAVPMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESFRNQILAYFDYVFTAIFTTEIALKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLF--KFYSCTDTTKMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIPKNQHQ--YKIWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDI------------------LNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDACPEVTPGSEDTSRI-SITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKSDFLPG-----EEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVSMNMPLNSDGTVTFNATLFSLVRTALKIKTEGNFEQSN--EELRMIIKKIWKR-TSMKLLDQVIP-PIGDDEVTVGKFYAIFLIQEHFRKFKK 1443 HSP 2 Score: 140.969 bits (354), Expect = 9.638e-33 Identity = 160/677 (23.63%), Postives = 299/677 (44.17%), Query Frame = 2
Query: 176 PNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQAC-----YPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLY---------FVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKK-ELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIIN--PKQSFG--------FSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFG--GDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDF----------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
P PD S FIF N R C ++ F ++L I+ + +SLA E P +F +A + +F IFTTE LK+ G + K ++ ++ +NILD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I L+ +F+ IG+++++ F+ +C T T++ C + S + + F +FDN+ M+++F T EGW +LY+ + +Y F++ I+L +FFM+N+ +G + ++ F+ + E K EL KN+++ ++ + YI K H+ + + + + + F + + L++LNT + ++HYGQ F IF E+ +K+ F + YF +NVFDF++V S+++ I I+ P+ + G + R R++RL+KL +R L+++ + S +++ + LL+ + FI ++GMQ+FG + Q NF TFP A++ +F TGE W E++ ++ S DF S Y YF+ + + ++N+F+A+ +D
Sbjct: 691 PMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAED--PIEPESFRNQILAYFDYVFTAIFTTEIALKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFK--FY-SCTDTTK-----MTEEECRGYFIVAKDGDSTQIQLRERIWTHSDF----HFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFV------------------------------------------IVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIP--------------------KNQHQYK--IWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDILNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDACPEVTPGSEDTSRISITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKS-DFLPGEEYTCGTSFAY-FYFISFYMLCAFLIINLFVAVIMD 1287 HSP 3 Score: 95.5153 bits (236), Expect = 7.461e-19 Identity = 77/286 (26.92%), Postives = 139/286 (48.60%), Query Frame = 2
Query: 212 NNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGI-EPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV----DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD-----SQWNVLYFVLLIVLGSFFMLNLVLGV 1039
+ ++RR C+ L++ R+F + VIL I+ N +++A E H ++ +TK I + L +FT E +LKI ALGF +Y S++N D VV G++ + L + + + R IR+LR FK+ SL ++ S++ ++ + + LL+ ++IFA++G++++ G F F+ T+ FDN +++TVFQ +T E W V+Y A + +YF++L V G++ +LN+ L +
Sbjct: 366 HRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHH----NQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGF----QSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFD-----------FEDTE-------------------------IRRSTFDNFPQSLITVFQILTGEDWNSVMYNGIMAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAI 607 HSP 4 Score: 72.0182 bits (175), Expect = 1.067e-11 Identity = 75/319 (23.51%), Postives = 135/319 (42.32%), Query Frame = 2
Query: 3374 SPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGF----LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF----------------GNIE----LNDDTQINQ---------------NN---NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ PFE +I++ I + I L I + +PE + ++ N ++Y+ F IFT E LK++AY F L + ++ WNI DF V L + L ++ + T GF LR FRV+R ++++ S++++L + I++ L + LL+ + IY I+G ++F +E + Q Q NN +F NF +L +++C T E W ++ G+++ + YF++ IL+ +F +LNL + V+ F
Sbjct: 2 TEPFEMIILLTIFANCIALAI--YLPMPED----------DTNSVNTILEKIEYI---FLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQINKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVIATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDA---------------------IGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEF 284
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Xenopus
Match: frrs1l (ferric-chelate reductase 1-like [Source:Xenbase;Acc:XB-GENE-6257538]) HSP 1 Score: 1022.69 bits (2643), Expect = 0.000e+0 Identity = 652/1522 (42.84%), Postives = 935/1522 (61.43%), Query Frame = 2
Query: 197 FIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ------------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKS--------EESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
F N R+SC ++E + FE +++LTI ANC++LA+ + +P+ D + T + IE IFL IFT EA LKI+A GFI+ +AYL++ WNILDF +V++GL T+ L+ +++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AI+G E++ G H TC+ + + AT + P S T G C+ +W GP G+ +FDN G+AMLTV+QCITMEGWT+VLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R AF KLRE Q + L GY++W+ AE + + + + +L + +T+ E + +++I S ++ W + +R + +K++ FYW VI ++L NT +A EH+ QP++L + A V L++F E+ +KI+ G + YF S FN FD VV ++E + N G S LRCIRLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LF+ I LLGMQ+FGG F+FE + + FD FP ++ITVF ILTGE+WN VMYNGI M M YF+++ + GNY LLNVFLAIAVDNL A+ +++ + ++ ++ + K +P E P+ P + +++K +P SS FIFGP N R +CH + N F FI++ I SS++LAAEDP+ S L+ + FL +M G LH GS+ R+ +NI+D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + ++K + NI++V L QF+F I VQLF GKF+ C D +K +E C+GYF D IQ ++RIW + F++DN+++ M++LFT++T EGWP +L +I + + GPI NY+ EIA+F+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+ +A+ +P RY+P N++ YK+W LV S FEYL+ +I L++I+L ++ + + E + S+ LN+AFT IFT E +LK+ A++ K +F DPWN+FDF+ V+GS+ DV LSE+ + E T+ I ++ F RLFRVMRL+K+L RG +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +L+LFRCATGE+W I++ GK C + G ++ CG+ F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLF+L+R +L+IK E N +ELR+ + K+W +D +P +TVGK YA LI +++R F K
Sbjct: 32 FCLGLQNPFRKSCINIVEWKPFEMIILLTIFANCIALAIYLPMPEDDTNSVNTILEKIEYIFLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQINKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVI--ATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQKLREQQAMDEDLRGYLDWITHAE--VMDPDMEQGDGFSQLEEGCSETESLYELE-GINKFI--------------SFLKQWRHWHRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHHNQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGFQSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDTEIRRSTFDNFPQSLITVFQILTGEDWNSVMYNGI---MAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAIAVDNL------------------------------------AEAENLTSA--QKAKAEERKRKKLEP--EIPISPRPRPLAELQLKEKAVPMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESFP--LIHWLLYRSFQCFLSQMT----TYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIPKNQHQ--YKIWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDI------------------LNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTPEPVVTPGSEDTSRI-SITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKSDFLPG-----EEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVSMNMPLNSDGTVTFNATLFSLVRTALKIKTEGNFEQSN--EELRMIIKKIWKR-TSMKLLDQVIP-PIGDDEVTVGKFYAIFLIQEHFRKFKK 1455 HSP 2 Score: 126.716 bits (317), Expect = 2.354e-28 Identity = 147/678 (21.68%), Postives = 281/678 (41.45%), Query Frame = 2
Query: 176 PNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDL-RTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQAC-----YPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLY---------FVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKK-ELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXY-----------WSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFG--GDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDF----------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
P PD S FIF N R C ++ F ++L I+ + +SLA E + I + FL TT G + K ++ ++ +NILD +VV + L++ +Q+ + + + R +RVLRP + ++ L+ V+ + A+ + I L+ +F+ IG+++++G F+ +C T T++ C + S + + F +FDN+ M+++F T EGW +LY+ + +Y F++ I+L +FFM+N+ +G + ++ F+ + E K EL KN+++ ++ + YI K H+ + + + + + F + + L++LNT + ++HYGQ F IF E+ +K+ F + YF +NVFDF++V S+++ I I+ + + + + +R L+++ + S +++ + LL+ + FI ++GMQ+FG + Q NF TFP A++ +F TGE W E++ ++ S DF S Y YF+ + + ++N+F+A+ +D
Sbjct: 706 PMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESFPLIHWLLYRSFQCFLSQMTT--------YGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFY-SCTDTTK-----MTEEECRGYFIVAKDGDSTQIQLRERIWTHSDF----HFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFV------------------------------------------IVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIP--------------------KNQHQYK--IWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDILNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDTPEPVVTPGSEDTSRISITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKS-DFLPGEEYTCGTSFAY-FYFISFYMLCAFLIINLFVAVIMD 1299
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Xenopus
Match: frrs1l (ferric-chelate reductase 1-like [Source:Xenbase;Acc:XB-GENE-6257538]) HSP 1 Score: 1003.82 bits (2594), Expect = 0.000e+0 Identity = 653/1513 (43.16%), Postives = 935/1513 (61.80%), Query Frame = 2
Query: 248 EQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ------------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSE-----------AKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSS-LFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKS-------EESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
E R FE +++LTI ANC++LA+ + +P+ D + T + IE IFL IFT EA LKI+A GFI+ +AYL++ WNILDF +V++GL T+ L+ +++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AI+G E++ G H TC+ + + AT + P S T G C+ +W GP G+ +FDN G+AMLTV+QCITMEGWT+VLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R AF KLRE Q + L GY++W+ AE + + + + +L + +T+ E + +++I S ++ W + +R + +K++ FYW VI ++L NT +A EH+ QP++L + A V L++F E+ +KI+ G + YF S FN FD VV ++E + N G S LRCIRLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LF+ I LLGMQ+FGG F+FE + + FD FP ++ITVF ILTGE+WN VMYNGI M M YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++ + ++ R F K ++I + EE + +P+ E + + P +P +S FIFGP N R +CH + N F FI++ I SS++LAAEDP+ S L+ + FL +M G LH GS+ R+ +NI+D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + ++K + NI++V L QF+F I VQLF GKF+ C D +K +E C+GYF D IQ ++RIW + F++DN+++ M++LFT++T EGWP +L +I + + GPI NY+ EIA+F+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+ +A+ +P RY+P N++ YK+W LV S FEYL+ +I L++I+L ++ + + E + S+ LN+AFT IFT E +LK+ A++ K +F DPWN+FDF+ V+GS+ DV LSE+ + E T+ I ++ F RLFRVMRL+K+L RG +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +L+LFRCATGE+W I++ GK C + G ++ CG+ F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLF+L+R +L+IK E N +ELR+ + K+W +D +P +TVGK YA LI +++R F K
Sbjct: 36 EYRPFEMIILLTIFANCIALAIYLPMPEDDTNSVNTILEKIEYIFLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQINKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVI--ATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVLYWVNDAIGNEWPWIYFVSLILLGSFFVLNLVLGVLSGEFTKEREKAKSRGAFQKLREQQAMDEDLRGYLDWITHAE--VMDPDMEQGDGFSQLEEGCSETESLYELE-GINKFI--------------SFLKQWRHWHRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHHNQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGFQSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFDFEDTEIRRSTFDNFPQSLITVFQILTGEDWNSVMYNGI---MAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAIAVDNLAEAENLTSAQKAKAEERKRKKLARLFCSSRGRFTKALTFKKKKLKIKKKKKNIQECDDEEEEPEIPISPRPRPLAELQLKEKAVP----------MPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESF--PLIHWLLYRSFQCFLSQM----TTYGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFYSCTDTTKMTEEECRGYFIVAKDGDSTQIQLRERIWTHSDFHFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIPKNQHQ--YKIWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDI------------------LNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDACPEVTPGSEDTSRI-SITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKSDFLPG-----EEYTCGTSFAYFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVSMNMPLNSDGTVTFNATLFSLVRTALKIKTEGNFEQSN--EELRMIIKKIWKR-TSMKLLDQVIP-PIGDDEVTVGKFYAIFLIQEHFRKFKK 1480 HSP 2 Score: 127.487 bits (319), Expect = 1.269e-28 Identity = 155/677 (22.90%), Postives = 291/677 (42.98%), Query Frame = 2
Query: 176 PNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDL-RTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQAC-----YPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLY---------FVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKK-ELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIIN--PKQSFG--------FSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFG--GDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDF----------SMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
P PD S FIF N R C ++ F ++L I+ + +SLA E + I + FL TT G + K ++ ++ +NILD +VV + L++ +Q+ + + + R +RVLRP + ++ L+ V+ + A+ + I L+ +F+ IG+++++G F+ +C T T++ C + S + + F +FDN+ M+++F T EGW +LY+ + +Y F++ I+L +FFM+N+ +G + ++ F+ + E K EL KN+++ ++ + YI K H+ + + + + + F + + L++LNT + ++HYGQ F IF E+ +K+ F + YF +NVFDF++V S+++ I I+ P+ + G + R R++RL+KL +R L+++ + S +++ + LL+ + FI ++GMQ+FG + Q NF TFP A++ +F TGE W E++ ++ S DF S Y YF+ + + ++N+F+A+ +D
Sbjct: 732 PMPDASSFFIFGPTNKFRVLCHRIVNATTFTNFILLFILLSSISLAAEDPIEPESFPLIHWLLYRSFQCFLSQMTT--------YGAFLHKGSFCRNYFNILDLLVVAVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIKTIGNIVLVTTLLQFMFSCIGVQLFKGKFY-SCTDTTK-----MTEEECRGYFIVAKDGDSTQIQLRERIWTHSDF----HFDNVLSGMMSLFTISTFEGWPQLLYRAIDSHSEDMGPIYNYRIEIAVFFIIYIILIAFFMMNIFVGFV------------------------------------------IVTFQEQGEQEYKNCELDKNQRQCVQYALKARPLRRYIP--------------------KNQHQYK--IWYLVTSSYFEYLMFALIMLNTISLGMQHYGQTAEFSHLSDILNVAFTGIFTVEMVLKLAAFKAKGYFGDPWNVFDFLIVIGSIIDVILSEIDACPEVTPGSEDTSRISITFFRLFRVMRLVKLLSRGEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLVLFRCATGEAWQEILLACSYGKLCDPKS-DFLPGEEYTCGTSFAY-FYFISFYMLCAFLIINLFVAVIMD 1324 HSP 3 Score: 95.5153 bits (236), Expect = 8.062e-19 Identity = 77/286 (26.92%), Postives = 139/286 (48.60%), Query Frame = 2
Query: 212 NNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIE-PIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV----DLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD-----SQWNVLYFVLLIVLGSFFMLNLVLGV 1039
+ ++RR C+ L++ R+F + VIL I+ N +++A E H ++ +TK I + L +FT E +LKI ALGF +Y S++N D VV G++ + L + + + R IR+LR FK+ SL ++ S++ ++ + + LL+ ++IFA++G++++ G F F+ T+ FDN +++TVFQ +T E W V+Y A + +YF++L V G++ +LN+ L +
Sbjct: 401 HRLLRRKCRDLVKSRFFYWFVILIILFNTITIATEHH----NQPEWLTKAQDIANQVLLAMFTLEMVLKIYALGF----QSYFMSLFNRFDCFVVCTGILEILLVSSNIMSPLGISVLRCIRLLRIFKITRYWTSLNNLVASLLNSVRSIASLLLLLFLFMIIFALLGMQMFGGKFD-----------FEDTE-------------------------IRRSTFDNFPQSLITVFQILTGEDWNSVMYNGIMAYGGPTFPGMFVCIYFIILFVCGNYILLNVFLAI 642 HSP 4 Score: 91.2781 bits (225), Expect = 1.665e-17 Identity = 71/258 (27.52%), Postives = 131/258 (50.78%), Query Frame = 2
Query: 2384 NFIRRVCHFV-ANLRFFDIFIMMVIFASSMALAAEDPVIFNSLR--NRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLN-------------TIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGK-----FFYCNDLSKRIKETCKGYFYTFNDQMGIQAKK-RIW----QRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
N +RR F+ + R F++ I++ IFA+ +ALA P+ + N +LE + F +F +E LK++ G +LH +Y+R+ WNI+D +V + +F+ + +N +K+LR RVLRPL+ ++ VP L+ V +II ++ + +I ++ + I+ ++ +LFSGK ++ D+ +++ Q I + R W ++DN +M+T++ T EGW +VL
Sbjct: 24 NPLRRKDPFLTSEYRPFEMIILLTIFANCIALAIYLPMPEDDTNSVNTILEKIEYIFLFIFTIEAALKIVAYGFILHSDAYLRNCWNILDFTIVSVGLFTIVLEQ-INKVQGVTAPGGTGFNVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFMIIIYAIVGKELFSGKMHKTCYYKATDVIATVEKPSPCSTTGQGRQCAINGSECRGWWPGPNNGITHFDNFGFAMLTVYQCITMEGWTEVL 280
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Mouse
Match: Cacna1s (calcium channel, voltage-dependent, L type, alpha 1S subunit [Source:MGI Symbol;Acc:MGI:88294]) HSP 1 Score: 1066.22 bits (2756), Expect = 0.000e+0 Identity = 670/1546 (43.34%), Postives = 968/1546 (62.61%), Query Frame = 2
Query: 176 PNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNL------QADT---------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXX-------------------XXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMY---DKPKDKKQPIKESPVIVENPPAEGIIVRKKILP---YSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P P R+LF + N +R++C ++E + FE +++LTI ANCV+LA+ + +P+ D + + +E FL +F+ EA +KIIA GF+ ++AYL+S WN+LDFI+V +G+ TV L Q +T +D++ RA RVLRP +LVSGVPSLQVVL SI KAM PL I LLVLF V+I+AIIGLE+++G H TC+ + + + P + T G C+ W GP G+ +FDN G++MLTV+QCI+MEGWTDVLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R F KLRE Q+ E L GYM+W+ T E++ ++ + E K L + T+ E + +K I +W ++ R +K++ FYW VI +V LNT +A EH+ QP +L A V L++F E+ +K++G G+R YF S FN FD VV ++E + G S LRCIRLLRL K+T+YW+SL NLV SLL+S+RSI SLLLLL LFI I LLGMQLFGG ++FE + NFD FP A+I+VF +LTGE+WN VMYNGI M ++ YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + + ++ E +++ ++ KI + + ++ E + Y D P D ++ E PV P + +++K +P SS FIF P N IR +CH + N +F FI++ I SS ALAAEDP+ +S+RN++LE+FD FT+VF +E++LK+ G LH GS+ R+ +NI+D++VV +++ S S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI++V L QF+F I VQLF GKF+ CNDLSK +E C+GY+Y + D I+ + R W N F++DN++++M++LFTV+T EGWP +L +I + + GP+ N + E+AIF+I++ I+ FF +NIFV +IVTFQEQGE++ + ELDKN+ +C+ +A+ +P Y+P KN Y+VW +V S FEYL+ +I L++I L ++ + QS Q + + LN+AFTIIFT E +LK+IA++ + +F DPWN+FDF+ V+GS+ DV LSE+ + + I + F RLFRVMRL+K+L R +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W I++ GK C + G ++ CG++F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ +WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + + ++ELR + K+W + + P+ +TVGK YA LI +++R F K
Sbjct: 25 PRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWI-TQGEVMDVDDLR--EGKLSLDEGGSDTESLYE---------------IEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGI---MAYGGPTYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERATVTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIP--KNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYN----------------QSEQMNHISDI--LNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDDPDESARISS-AFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPG-----EEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIK--TEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDE--VTVGKFYATFLIQEHFRKFMK 1519 HSP 2 Score: 102.449 bits (254), Expect = 4.793e-21 Identity = 89/314 (28.34%), Postives = 149/314 (47.45%), Query Frame = 2
Query: 2234 IEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIF--NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSK----NLNT-----------IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGK-----FFYCNDLSKRIK--------ETCKGYFYTFNDQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
+E + D+ KKQP K P I+ PP +LF N +R+ C + + F+ I++ IFA+ +ALA P+ N+ N LE + F VF +E +K+I G + H +Y+R WN++D I+V + VF+ + NT +K+LR RVLRPL+ ++ VP L+ V +I ++ +F+I ++ + I+ +I ++LF GK +F D+ ++ T G T N G + + N+ ++DN SM+T++ + EGW DVL
Sbjct: 1 MEPPSPQDEGLRKKQPKKPVPEILPRPP-------------RALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTIN---GSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVL 298
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Mouse
Match: Cacna1c (calcium channel, voltage-dependent, L type, alpha 1C subunit [Source:MGI Symbol;Acc:MGI:103013]) HSP 1 Score: 1065.83 bits (2755), Expect = 0.000e+0 Identity = 685/1587 (43.16%), Postives = 964/1587 (60.74%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKT----DSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN--------------------------------WIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXFGSEAKIQ------DIDDSIIEELNMYD-KPKDKKQPIKES---------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY N A W +YFV LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + ++ ++ S + S E+ W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD +V ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +++ E+ E KI+ D + ++NM D +P + + S PV P + +++K +P S+ FIF P+N R CH + N F I+ I SS++LAAEDPV S RN +L +FD+ FT++F +E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE S E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEDKPRNRGAPAGLHDQKKGKFAWFSHSTETHVSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNSAEENSRI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1647 HSP 2 Score: 104.375 bits (259), Expect = 1.132e-21 Identity = 74/260 (28.46%), Postives = 135/260 (51.92%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E LK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 898 PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1149
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Mouse
Match: Cacna1c (calcium channel, voltage-dependent, L type, alpha 1C subunit [Source:MGI Symbol;Acc:MGI:103013]) HSP 1 Score: 1065.06 bits (2753), Expect = 0.000e+0 Identity = 686/1572 (43.64%), Postives = 957/1572 (60.88%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKT----DSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXFGSEAKIQ------DIDDSIIEELNMYD-KPKDKKQPIKES---------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY N A W +YFV LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD +V ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +++ E+ E KI+ D + ++NM D +P + + S PV P + +++K +P S+ FIF P+N R CH + N F I+ I SS++LAAEDPV S RN +L D FTS+F LE+ILK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1633 HSP 2 Score: 109.383 bits (272), Expect = 3.604e-23 Identity = 75/260 (28.85%), Postives = 136/260 (52.31%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E ILK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 873 PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1124
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Mouse
Match: Cacna1c (calcium channel, voltage-dependent, L type, alpha 1C subunit [Source:MGI Symbol;Acc:MGI:103013]) HSP 1 Score: 1057.74 bits (2734), Expect = 0.000e+0 Identity = 683/1575 (43.37%), Postives = 962/1575 (61.08%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKT----DSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIF---QIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXFGSEAKIQ------DIDDSIIEELNMYD-KPKDKKQPIKES---------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD +V ++E I +I++P G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +++ E+ E KI+ D + ++NM D +P + + S PV P + +++K +P S+ FIF P+N R CH + N F I+ I SS++LAAEDPV S RN +L +FD+ FT++F +E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 130 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSP---LGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1663 HSP 2 Score: 104.375 bits (259), Expect = 1.365e-21 Identity = 74/260 (28.46%), Postives = 135/260 (51.92%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E LK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 903 PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1154
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Mouse
Match: Cacna1c (calcium channel, voltage-dependent, L type, alpha 1C subunit [Source:MGI Symbol;Acc:MGI:103013]) HSP 1 Score: 1057.36 bits (2733), Expect = 0.000e+0 Identity = 685/1575 (43.49%), Postives = 960/1575 (60.95%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKT----DSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIF---QIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXFGSEAKIQ------DIDDSIIEELNMYD-KPKDKKQPIKES---------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD +V ++E I +I++P G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +++ E+ E KI+ D + ++NM D +P + + S PV P + +++K +P S+ FIF P+N R CH + N F I+ I SS++LAAEDPV S RN +L D FTS+F LE+ILK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 130 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSP---LGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1663 HSP 2 Score: 109.768 bits (273), Expect = 3.354e-23 Identity = 75/260 (28.85%), Postives = 136/260 (52.31%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E ILK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 903 PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1154
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. UniProt/SwissProt
Match: sp|P91645|CAC1A_DROME (Voltage-dependent calcium channel type A subunit alpha-1 OS=Drosophila melanogaster OX=7227 GN=cac PE=1 SV=3) HSP 1 Score: 1316.6 bits (3406), Expect = 0.000e+0 Identity = 810/1534 (52.80%), Postives = 1052/1534 (68.58%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCN---------AGFTCK-------PYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN-DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQND-FCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELND-DTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P + +S T CN F C W+GP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEF+ ER RVE+R F K R ++ + Y++W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L +++ D ++ + K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H Q D + SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E ++S + N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + I ++NNF++F+ G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WR+
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDP-----NKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFSNERNRVERRMEFQKCRFRAMFQTAMVSYLDWITQAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPSFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAQKG----MIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQADGVHVENGDGAVAPSKSKGKK---KEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHN-------------------QGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNSI--NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADMELRETITNIWP-LQAKKMLNLLVPPSDQLNKGK--LSVGKIYAGFLILESWRS 1496 HSP 2 Score: 155.606 bits (392), Expect = 2.340e-36 Identity = 164/682 (24.05%), Postives = 300/682 (43.99%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQS----FGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG------KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD A Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ S GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG N + G NF +F ++ +F TGE W +M ++ + K S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSAECQGSYFKYEEDELLPKQELRVWKPRAF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGDMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG---NIKLGTVENSITRHNNFQSFIQGVMLLFRCATGEAWPNIMLACLKGKACDDDAEKAPGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1338 HSP 3 Score: 101.293 bits (251), Expect = 7.621e-20 Identity = 83/284 (29.23%), Postives = 135/284 (47.54%), Query Frame = 2
Query: 2291 SPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIK--------------ETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVL 3079
P ENPP G +SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +GLVLH SY+R+ WNIMD VV+ + + +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D +K +K E G F N K ++DNI +M+T+F T EGW +L
Sbjct: 3 GPKKEENPPGGGP---------TSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPGGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQIGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAIL 277 HSP 4 Score: 67.0106 bits (162), Expect = 1.953e-9 Identity = 79/347 (22.77%), Postives = 135/347 (38.90%), Query Frame = 2
Query: 3308 PTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFD-FVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI----------------ELNDDTQINQNN------------------------------NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFD 4195
PTS ++ N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A L + ++ WNI D FV V G +T + + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + E +T N +N +F N +L +F+C T E W I+ T D L GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 15 PTSLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------GGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMT-------QYPQIGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNILEKATGSFVCNNTTSMCLEKWEGPNSGITSFDNIGFAMLTVFQCITMEGWTAILYWT--------------------NDAL-GSAFNWIYFVPLIVIGSFFMLNLVLGVLSGEFS 317
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. UniProt/SwissProt
Match: sp|P07293|CAC1S_RABIT (Voltage-dependent L-type calcium channel subunit alpha-1S OS=Oryctolagus cuniculus OX=9986 GN=CACNA1S PE=1 SV=1) HSP 1 Score: 1061.6 bits (2744), Expect = 0.000e+0 Identity = 673/1565 (43.00%), Postives = 969/1565 (61.92%), Query Frame = 2
Query: 176 PNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNL------QADT---------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS-------------------EAKIQDIDDSIIEELNMY---DKPKDKKQPIKESPVIVENPPAEGIIVRKKILP---YSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKS-------------------EESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P P R+LF + N +R++C ++E + FE +++LTI ANCV+LA+ + +P+ D + + +E FL +F+ EA +KIIA GF+ ++AYL+S WN+LDFI+V +G+ T L Q++T +D++ RA RVLRP +LVSGVPSLQVVL SI KAM PL I LLVLF V+I+AIIGLE+++G H TC+ + + + P + T G C+ W GP G+ +FDN G++MLTV+QCITMEGWTDVLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R F KLRE Q+ E L GYM+W+ T E++ E+ + E K L + T+ E + +K I +W ++ R +K++ FYW VI +V LNT +A EH+ QP +L A V LS+F E+ +K++G G+R YF S FN FD VV ++E + G S LRCIRLLRL K+T+YW+SL NLV SLL+S+RSI SLLLLL LFI I LLGMQLFGG ++FE + NFD FP A+I+VF +LTGE+WN VMYNGI M ++ YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++ + R+ ++ + S + K+ + + ++ E + Y D P D ++ E PV P + +++K +P SS FIF P N +R +CH + N +F FI++ I SS ALAAEDP+ S+RN++L +FD+AFTSVF +E++LK+ G LH GS+ R+ +NI+D++VV +++ S S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI++V L QF+F I VQLF GKFF CNDLSK +E C+GY+Y + D ++ + R W N F++DN++++M++LFTV+T EGWP +L +I + + GP+ N + E+AIF+I++ I+ FF +NIFV +IVTFQEQGE++ + ELDKN+ +C+ +A+ +P Y+P KN Y+VW +V S FEYL+ +I L++I L ++ + + E S+ LN+AFTIIFT E ILK++A++ + +F DPWN+FDF+ V+GS+ DV LSE+ + + + I + F RLFRVMRLIK+L R +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I L D TQIN+NNNF+ F +LLLFRCATGE+W I++ GK C + G ++ CG++F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ +WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + + ++ELR + K+W + + P+ +TVGK YA LI +++R F K
Sbjct: 25 PRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLTVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNVIQSNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYYIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWI-TQGEVMDVEDLR--EGKLSLEEGGSDTESLYE---------------IEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSRVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDIANRVLLSLFTIEMLLKMYGLGLRQYFMSIFNRFDCFVVCSGILELLLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGI---MAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKTEEEKSVMAKKLEQKPKGEGIPTTAKLKVDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKVRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRAESVRNQILGYFDIAFTSVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSTISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFFSCNDLSKMTEEECRGYYYVYKDGDPTQMELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYRAIDSNEEDMGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIP--KNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYHQSEEMNHISDI------------------LNVAFTIIFTLEMILKLLAFKARGYFGDPWNVFDFLIVIGSIIDVILSEIDTFLASSGGLYCLGGGCGNVDPDESARISS-AFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPG-----EEYTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIK--TEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDE--VTVGKFYATFLIQEHFRKFMK 1538 HSP 2 Score: 102.064 bits (253), Expect = 3.832e-20 Identity = 88/314 (28.03%), Postives = 150/314 (47.77%), Query Frame = 2
Query: 2234 IEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIF--NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSK----NLNT-----------IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGK-----FFYCNDLSKRIK--------ETCKGYFYTFNDQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
+E + D+ KKQP K P ++ PP +LF N +R+ C + + F+ I++ IFA+ +ALA P+ N+ N LE + F +VF +E +K+I G + H +Y+R WN++D I+V + VF+ + NT +K+LR RVLRPL+ ++ VP L+ V +I ++ +F+I ++ + I+ +I ++LF GK ++ D+ ++ T G T N G + + N+ ++DN SM+T++ T EGW DVL
Sbjct: 1 MEPSSPQDEGLRKKQPKKPLPEVLPRPP-------------RALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPEDDNNSLNLGLEKLEYFFLTVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNVIQSNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYYIGTDIVATVENEKPSPCARTGSGRPCTIN---GSECRGGWPGPNHGITHFDNFGFSMLTVYQCITMEGWTDVL 298
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. UniProt/SwissProt
Match: sp|P15381|CAC1C_RABIT (Voltage-dependent L-type calcium channel subunit alpha-1C OS=Oryctolagus cuniculus OX=9986 GN=CACNA1C PE=1 SV=1) HSP 1 Score: 1056.97 bits (2732), Expect = 0.000e+0 Identity = 681/1572 (43.32%), Postives = 956/1572 (60.81%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWK----TDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXFGSEAKIQ------DIDDSIIEELNMYD-KPKDK--KQPIKES-------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD +V ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +++ + E KI+ D + ++NM D +P + K P PV P + +++K +P S+ FIF P+N R CH + N F I+ I SS++LAAEDPV S RN +L +FD+ FT++F +E+ LK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 130 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGVADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEEKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKVMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVGKPALEEAKEEKIELKSITADGESPPTTKINMDDLQPNESEDKSPYPNPETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPHNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1663 HSP 2 Score: 103.605 bits (257), Expect = 1.360e-20 Identity = 74/260 (28.46%), Postives = 135/260 (51.92%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E LK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 903 PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1154
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. UniProt/SwissProt
Match: sp|Q02485|CAC1S_RAT (Voltage-dependent L-type calcium channel subunit alpha-1S OS=Rattus norvegicus OX=10116 GN=Cacna1s PE=1 SV=2) HSP 1 Score: 1056.2 bits (2730), Expect = 0.000e+0 Identity = 671/1565 (42.88%), Postives = 969/1565 (61.92%), Query Frame = 2
Query: 176 PNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNL------QADT---------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNA-----GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGS-------------------EAKIQDIDDSIIEELNMY---DKPKDKKQPIKESPVIVENPPAEGIIVRKKILP---YSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF--NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKS-------------------EESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P P R+LF + N +R++C ++E + FE +++LTI ANCV+LA+ + +P+ D + + +E FL +F+ EA +KIIA GF+ ++AYL+S WN+LDFI+V +G+ T L Q +T +D++ RA RVLRP +LVSGVPSLQVVL SI KAM PL I LLVLF V+I+AIIGLE+++G H TC+ + + + P + T G C+ W GP G+ +FDN G++MLTV+QCI+MEGWTDVLY N A ++W +YFV LI+LGSFF+LNLVLGVLSGEF KERE+ + R F KLRE Q+ E L GYM+W+ T E++ ++ + E K L + T+ E + +K I +W ++ R +K++ FYW VI +V LNT +A EH+ QP +L A V L++F E+ +K++G G+R YF S FN FD VV ++E + G S LRCIRLLRL K+T+YW+SL NLV SLL+S+RSI SLLLLL LF+ I LLGMQLFGG ++FE + NFD FP A+I+VF +LTGE+WN VMYNGI M ++ YF+++ + GNY LLNVFLAIAVDNLA A L++A++ + ++ + R+ ++ + S + KI + + ++ E + Y D P D ++ E P P + +++K +P SS FIF P N IR +CH + N +F FI++ I SS ALAAEDP+ +S+RN++LE+FD FT+VF +E++LK+ G LH GS+ R+ +NI+D++VV +++ S S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI++V L QF+F I VQLF GKF+ CNDLSK +E C+GY+Y + D I+ + R W N F++DN++++M++LFTV+T EGWP +L +I + + GP+ N + E+AIF+I++ I+ FF +NIFV +IVTFQEQGE++ + ELDKN+ +C+ +A+ +P Y+P KN Y+VW +V S FEYL+ +I L++I L ++ + QS Q + + LN+AFTIIFT E ILK+IA++ + +F DPWN+FDF+ V+GS+ DV LSE+ + + + I + F RLFRVMRLIK+L R +R LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W I++ GKRC + G ++ CG++F+Y YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ +WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + + ++ELR + K+W + + P+ +TVGK YA LI +++R F K
Sbjct: 25 PRPPRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWI-TQGEVMDVDDLR--EGKLSLDEGGSDTESLYE---------------IEGLNKIIQFIRHWRQWNRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMTPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGI---MAYGGPSYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMTKKLEQKPKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPASPRPRPLAELQLKEKAVPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIP--KNPYQYQVWYVVTSSYFEYLMFALIMLNTICLGMQHYN----------------QSEQMNHISDI--LNVAFTIIFTLEMILKLIAFKPRGYFGDPWNVFDFLIVIGSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESARISS-AFFRLFRVMRLIKLLSRAEGVRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKRCDPESDYAPG-----EEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIK--TEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIGDDE--VTVGKFYATFLIQEHFRKFMK 1538 HSP 2 Score: 102.449 bits (254), Expect = 3.198e-20 Identity = 90/314 (28.66%), Postives = 149/314 (47.45%), Query Frame = 2
Query: 2234 IEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIF--NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSK----NLNT-----------IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGK-----FFYCNDLSKRIK--------ETCKGYFYTFNDQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
+E + D+ KKQP K P I+ PP +LF N +R+ C V + F+ I++ IFA+ +ALA P+ N+ N LE + F VF +E +K+I G + H +Y+R WN++D I+V + VF+ + NT +K+LR RVLRPL+ ++ VP L+ V +I ++ +F+I ++ + I+ +I ++LF GK +F D+ ++ T G T N G + + N+ ++DN SM+T++ + EGW DVL
Sbjct: 1 MEPSSPQDEGLRKKQPKKPVPEILPRPP-------------RALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTIN---GSECRGGWPGPNHGITHFDNFGFSMLTVYQCISMEGWTDVL 298
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. UniProt/SwissProt
Match: sp|Q01815|CAC1C_MOUSE (Voltage-dependent L-type calcium channel subunit alpha-1C OS=Mus musculus OX=10090 GN=Cacna1c PE=1 SV=1) HSP 1 Score: 1056.2 bits (2730), Expect = 0.000e+0 Identity = 683/1572 (43.45%), Postives = 955/1572 (60.75%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKT----DSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXX-------XXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXX---------XXXXXXXXXXXXXXXXXXXFGSEAKIQ------DIDDSIIEELNMYD-KPKDKKQPIKES---------------PVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG----------FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+L + N IRR+C ++E + FE +++LTI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ D P D + A C G CKP WDGP G+ NFDN +AMLTVFQCITMEGWTDVLY A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N +E + K R + E + E + + W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A L++F E+ +K++ G++ YF S FN FD +V ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +++ E+ E KI+ D + ++NM D +P + + S PV P + +++K +P S+ FIF P+N R CH + N F I+ I SS++LAAEDPV S RN +L D FTS+F LE+ILK+ G LH GS+ R+ +NI+D++VV +++ SF S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GK + C+D SK+ + CKG + T+ D + R W+ + F++DN++ +M+ LFTV+T EGWP++L SI + ++GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P N++ YKVW +V S FEYL+ ++I L++I L ++ + QS F +++ LN+ FT +FT E ILK+IA++ K +F DPWN+FDF+ V+GS+ DV LSE E T P++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I LND T+IN+NNNF+ F +LLLFRCATGE+W IM+ GK+CA N T G + CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 100 PPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIIDVPAEEDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEDEGMDEDKPRNMSMPTSETESVNTENVAGGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVMEKPAVEESKEEKIELKSITADGESPPTTKINMDDLQPSENEDKSPHSNPDTAGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQ--YKVWYVVNSTYFEYLMFVLILLNTICLAMQHYG----------------QSCL--FKIAMNILNMLFTGLFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNPAEHTQCSPSMSAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPSNSTEG-------ETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TEGNLEQANEELRAIIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1633 HSP 2 Score: 109.768 bits (273), Expect = 2.337e-22 Identity = 75/260 (28.85%), Postives = 136/260 (52.31%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY-PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
P P+ S FIFS NN R C ++ F L++ I+ + +SLA E P +F + + +F IFT E ILK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G + TC +DS +A + Y + + W+ F +FDN+ AM+ +F T EGW ++LY++
Sbjct: 873 PMPEASAFFIFSPNNRFRLQCHRIVNDTIFTNLILFFILLSSISLAAED--PVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLY-TC--SDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKF---DFDNVLAAMMALFTVSTFEGWPELLYRS 1124
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. TrEMBL
Match: A0A0X3P936 (EF-hand domain-containing protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR160159 PE=3 SV=1) HSP 1 Score: 1390.17 bits (3597), Expect = 0.000e+0 Identity = 843/1529 (55.13%), Postives = 1081/1529 (70.70%), Query Frame = 2
Query: 185 DRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT-VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDAT-------------QQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIE---NWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIE---ELNMYDKPKDKKQPIKESPVIVENPPAEGIIVR----KKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELND-DTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNK--ASYPLTVGKIYAGMLIMKNW 4651
+R+LF+FSE N++R++ K +I+ FEY+V++TI ANCV LA+EVHLP DR + T+M +E FLGIFT EAILKI+ALGF++ +YL++VWN LDF+VV G +TV T VDLRT RA+RVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLF V+IFAIIGLE Y G FH TC+ +P + AT + C G+ CK YW+GP +G+ +FDNIGYAMLTVFQCITMEGWTD++ T+ A +++N LYF+ LIVLGSFF+LNLVLGVLSGEFAKERERVEKRRAFLKLR Q+ E LDGY++W+ AEE+I E + ++ +++ R++ ++ R+ R Y +L D+ + + + +Y+ R + K+Q FY V+ +V LNT CVA+EHYGQP +L RFL+YAE++FL +FM E+ +K+F G YF+S FN+FDF VV S E I+Q+ P SFGFSALR IRLLR+ K TRYW+SLRNLV SLL+SMRSI+SLL LL LF+ I LLGMQLFGG F F G+P+ +FDTF A++TVF ILTGE+WN +MYNGIRSQ S +YS YF+L++IFGNYTLLNVFLAIAVDNLANA EL+A EE E K + E E ++ S A+ D++ I++ E + P+ +SP I ++ R K +LPYSS++IF P N RR CHFV NLR+FD+FIM+VI +SS++LAAEDPV NS RN +LE+FD AFT VF +EMILK++D+G+V+HP SY RD WNI+D +VV+ A+ +FF SKNL TIKSLRV+RVLRPLKTI RVPKLKAVF+ ++SS+KNVFNILIV++LFQFIFGVIAVQLF GKFFYCNDLSK +E C+GYF ++ D K RIW FNYDNI+ +M+TLFTVTTGEGWPDV+KNSI AT N GP +++Q++AIFY+ FFIVFPFFFVNIFVALII+TFQEQGE++ D ELDKN+ +CIDFAIN +P RYMP + S Y+VW LVVS PFEY IM +IAL+++ L++K H PER+ + +C +L YLN AFT +FT EC+LK++A+ K +F+D WNIFDF+TV+GS+TDV +S ++ + NLGFLRLFR RL+K+L +GY+IRILLWTFIQS KALP+VCLLI +LFFIY IIGMQ+FGNI+++D D+Q+N NFR+F LLLLFRCATGE+W +M+ ++ K CA + + CGS +Y YF+TFI + +F+MLNLFVAVIMDNFDYLTRD SILG HHLDE++R+WAE+DP TG ++ DM +M++KL PPVGFG CPYR A KLIRMN+ +D V F +TLFALIRESL IK+G + +D D ELR + +WP L + + +P + +T GKIYAG+LI +NW
Sbjct: 85 NRALFVFSEKNLLRKTAKTIIDWGPFEYMVLMTITANCVCLAMEVHLPNNDRRQLSTRMESLENYFLGIFTCEAILKIVALGFLLHPGSYLRNVWNFLDFVVVITGYVTVFAATGTGVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFGVVIFAIIGLEFYGGGFHLTCFDELNPHVLPATIPTIGKLVPCVITNGSVRTPGAFVCPEGYVCKGYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIMSVTDHAFGNRFNWLYFLPLIVLGSFFILNLVLGVLSGEFAKERERVEKRRAFLKLRRQQQTERELDGYIDWIHRAEEVILAEEQTTMADRIRILRARRRAEKLCGRRQRGHRYSEIDLQGLELGDRKRFRLGGSGSCCRYLKHFRLKCRTMAKSQTFYLIVLIVVGLNTVCVAIEHYGQPVWLSRFLYYAEFMFLGLFMAEMLLKLFSLGPTVYFQSSFNIFDFTVVIASFFEVIWQVFYPADSFGFSALRSIRLLRIFKFTRYWASLRNLVLSLLNSMRSIVSLLFLLFLFMLIFALLGMQLFGGGFAFMDGQPSQHFDTFAKALLTVFQILTGEDWNTIMYNGIRSQSGGST------VYSIYFVLLMIFGNYTLLNVFLAIAVDNLANAQELTAVEEAEMKIAAQKMENERQERLVQM--SLAEANDLNSDILKPEMESSTVGFNLTPSSPVHDSPPIDSVNDSDHSSGRLGYGKAMLPYSSMYIFSPTNPFRRFCHFVVNLRYFDLFIMIVIASSSISLAAEDPVNENSNRNIILEYFDHAFTCVFTIEMILKLVDLGVVMHPHSYFRDLWNIVDAVVVLAALVAFFSRQTDSSGIGRKTPSKNLGTIKSLRVLRVLRPLKTIRRVPKLKAVFDCVVSSLKNVFNILIVYLLFQFIFGVIAVQLFQGKFFYCNDLSKETREECQGYFISYEDNEP-AVKPRIWSSRSFNYDNIIFAMLTLFTVTTGEGWPDVMKNSIDATEVNRGPRTDHRQQMAIFYVFFFIVFPFFFVNIFVALIIITFQEQGENELVDHELDKNQKQCIDFAINARPLCRYMPEDVKSFQYRVWQLVVSGPFEYFIMTMIALNTLILMMKYHK--PERSITFPLVIDVNTRSYESYCSALMYLNTAFTCMFTVECLLKIMAFGPKNYFRDRWNIFDFITVIGSVTDVLVSGLQD----SSFLNLGFLRLFRAARLVKLLRQGYTIRILLWTFIQSIKALPYVCLLIAMLFFIYAIIGMQVFGNIDIDDPDSQLNDQTNFRSFANSLLLLFRCATGEAWQELMLSSDYPKPCA-----------NKPENACGSGIAYVYFVTFIFLCSFIMLNLFVAVIMDNFDYLTRDSSILGSHHLDEFIRVWAEYDPGATGRIHHTDMLEMLRKLEPPVGFGMKCPYRFAYRKLIRMNIPVDEAGTVPFTTTLFALIRESLSIKVGPASVMDIKDAELRETLRAMWP-LQAKKVANVLVPSDAELTCGHMTTGKIYAGLLIYENW 1586 HSP 2 Score: 110.153 bits (274), Expect = 4.995e-20 Identity = 81/281 (28.83%), Postives = 141/281 (50.18%), Query Frame = 2
Query: 167 GQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVT---------------IGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPY-WDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD 961
G+ P S++IFS N RR C ++ R+F+ +++ I ++ +SLA E + + I+ + + F +FT E ILK++ LG ++ ++Y + +WNI+D +VV IG T + T+ ++ R +RVLRP K + VP L+ V ++ ++ + I ++ L IF +I +++++G F + D L T++ C + + KP W F N+DNI +AMLT+F T EGW DV+ + AT+
Sbjct: 818 GKAMLPYSSMYIFSPTNPFRRFCHFVVNLRYFDLFIMIVIASSSISLAAEDPVNENSNRNIILEY--FDHAFTCVFTIEMILKLVDLGVVMHPHSYFRDLWNIVDAVVVLAALVAFFSRQTDSSGIGRKTPSKNLGTI--KSLRVLRVLRPLKTIRRVPKLKAVFDCVVSSLKNVFNILIVYLLFQFIFGVIAVQLFQGKFF---YCND---LSKETREECQGYFISYEDNEPAVKPRIWSSRSF---NYDNIIFAMLTLFTVTTGEGWPDVMKNSIDATE 1085
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. TrEMBL
Match: A0A4E0RMB3 (Voltage dependent calcium channel OS=Fasciola hepatica OX=6192 GN=D915_001184 PE=3 SV=1) HSP 1 Score: 1385.55 bits (3585), Expect = 0.000e+0 Identity = 849/1546 (54.92%), Postives = 1095/1546 (70.83%), Query Frame = 2
Query: 260 FEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT-VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQ------ACYPQSNT-------SCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXX-------IENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDID---DSIIEELNMYDKPKDKK-----------QPIKESPVIVENPP---------------AEGIIVR---KKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYS------------KNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIK----SHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDT-QINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYP---LTVGKIYAGMLIMKNWR----NFNK 4666
FEY+V+ TIIANCV LA+EVHLP D+ + K+ E F+GIFT EA LKIIALGFI+ +YL++VWNILDF+VV G +T+ + + + V+LRT RA+RVLRP KLVSGVPSLQVVLTSI+KAMAPLLQIG+LVLFA+LIFAI+GLE Y G+FH TC++ D+P + C P+ ++ SC +TCK YW+GP +G+ +FDNIGYAMLTVFQCIT+EGWTDVLY TN+A + NV+YF+ LIV+G+F ++NLVLGVLSGEFAKERERVEKRRAFLKLR Q+ + L+GY++W++ AEE+I E + + EK ++K R++ E L R+ E ++ + + K + K R V++ +K+Q FY V+ LVLLNT CV+VE YGQP +L FL YAE+VFL++FM E+ +KI+G G YF+S FN+FDF+VV SL E I+QI P SFGFSALR IRLLR+ K+TRYW+SLRNLV SLL++MRSI+SLL LL LF+ I LLGMQLFGG+FNF+ GKP NFDTF A++TVF ILTGE+WN +MY+GIRSQ SN +Y YF+L+V+FGNYTLLNVFLAIAVDNLANA EL+AAEEEE + + EE N GS A ++ D S+I+EL K D+ V+V+N P E I + K +LPYSS+FIF N +RR CHFV NLRFFD+FIM+VI +SS+ALAAEDPV NS+RN LLE+FD AFT VF +EM+LK+ID+G+ LHP SY RD WN++D VVI A+ SFF S KNL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ ++SS+KNV IL+V+ILFQFIF V+AVQLF GKFFYC D SK E C+GY++++ + K R+W F+YDN++++M+TLFTVTTGEGWP +LKNS+ AT N GP E+Y+Q++AIFYIVFFIVFPFFFVNIFVALII+TFQEQGE++ + ELDKN+ +C++FAIN KP RYMP+N++S+ Y++W LVVS PFEY IM++IAL+++ L++K HT+ S T L Q QN +C +L YLN AFT +FT EC+LK++A+ K +F+D WN FDF+TV+GS+TDV +SE++ + +LGFLRLFR RLIK++ +GY+IRILLWTFIQSFKALP+V LLI +LFFIY ++GMQ+FG I +ND+ QI NNF +F+ LLLFRCATGESW +M+ AC T G C++ CGS SY YF++F + +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE++R+WAE+DP TG ++ +MY+M++KL PPVGFG+NCPYR A KLIRMNM +D+Q V+F +TLFAL+RE+L IKM V +D D ELR + +WP + + ++D P + + Y LTVGKIYAG+LI++NW+ N NK
Sbjct: 16 FEYMVLFTIIANCVCLAMEVHLPCKDKRPLSEKLEMTEKYFMGIFTCEASLKIIALGFILHSGSYLRNVWNILDFVVVITGFLTMFMTSGSGVNLRTLRAVRVLRPLKLVSGVPSLQVVLTSILKAMAPLLQIGILVLFAILIFAIVGLEFYSGVFHVTCFREDNPTELPTSIPDAPGLIPCQPKDSSTRPVGAFSCPPNYTCKGYWEGPNYGITSFDNIGYAMLTVFQCITLEGWTDVLYMTNRAYGPRVNVIYFIPLIVIGAFLLINLVLGVLSGEFAKERERVEKRRAFLKLRRQQQSDRELNGYLDWIQKAEEVILAEEQTSFAEKLRIIKARKRA-EKLRRRSNDAEKQAIAAELENFEAELKEKRRFRLPGTGRFFKCQKRFRFVIRRLVKSQKFYIAVVVLVLLNTLCVSVEFYGQPSWLTEFLSYAEFVFLAVFMCEMVLKIYGLGGVLYFQSSFNIFDFMVVIASLFEIIWQIFYPSDSFGFSALRSIRLLRIFKITRYWASLRNLVLSLLNAMRSILSLLFLLFLFMLIFALLGMQLFGGEFNFDDGKPGQNFDTFVKALLTVFQILTGEDWNAIMYDGIRSQGGVSNG---GFVYCIYFILLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEEKRLADQLAEEENNAV-----GSSASGENEDGPNKSVIQELEDNKKSTDEAFRRNLLNDLEAGNHSNHTVVVQNLPLQMGQTQADSEAANNPETIRPQTYGKPMLPYSSMFIFSSTNPVRRFCHFVVNLRFFDLFIMIVIASSSLALAAEDPVSENSVRNCLLEYFDHAFTCVFAIEMLLKLIDLGVFLHPNSYCRDTWNLLDAAVVIGALISFFRSYHFGGKASHAGGKNLSTIKSLRVLRVLRPLKTIRRVPKLKAVFDCVVSSLKNVGIILVVYILFQFIFSVVAVQLFQGKFFYCTDQSKYTVEQCQGYYFSYEEGPIPVVKPRVWSSRDFHYDNVISAMLTLFTVTTGEGWPGILKNSMDATEVNHGPREDYQQQMAIFYIVFFIVFPFFFVNIFVALIIITFQEQGENELVEHELDKNQKQCMEFAINAKPLCRYMPSNRDSIKYRIWRLVVSSPFEYYIMMMIALNTLILMMKYYRPDHTET-----GSTTPDLDTQKYQN-YCSTLVYLNTAFTAMFTMECLLKLVAFGPKSYFRDWWNTFDFITVVGSITDVLVSELQD----SSFLSLGFLRLFRAARLIKLIRQGYTIRILLWTFIQSFKALPYVLLLIAMLFFIYAVVGMQVFGTIAINDEVGQITTYNNFHSFLNAALLLFRCATGESWQEVML--------AC---TDGQECVNKTAASCGSSVSYAYFVSFNFLCSFLMLNLFVAVIMDNFDYLTRDSSILGSHHLDEFIRVWAEYDPGATGRIHHTEMYEMLRKLEPPVGFGQNCPYRLAYRKLIRMNMPVDSQGTVHFTTTLFALVREALCIKMAPVELMDQKDAELRETIRTLWP-VQAKRKLDLLQPPD-SEYTFTHLTVGKIYAGLLILENWQLNRVNVNK 1529 HSP 2 Score: 102.449 bits (254), Expect = 9.656e-18 Identity = 81/297 (27.27%), Postives = 140/297 (47.14%), Query Frame = 2
Query: 143 QTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV---------NLQADTVDLRT---FRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVL---IFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGL---------VNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD 961
+T+ G+ P S+FIFS N +RR C ++ R+F+ +++ I ++ SLAL P + + + + F +F E +LK+I LG + N+Y + WN+LD VV LI+ A +L T R +RVLRP K + VP L+ V ++ + L +G++++ +L IF+++ +++++G F F T Q+ Y + C + Y +GP + ++DN+ AMLT+F T EGW +L + AT+
Sbjct: 744 ETIRPQTYGKPMLPYSSMFIFSSTNPVRRFCHFVVNLRFFDLFIMIVIASS--SLALAAEDPVSENSVRNCLLEYFDHAFTCVFAIEMLLKLIDLGVFLHPNSYCRDTWNLLDAAVVIGALISFFRSYHFGGKASHAGGKNLSTIKSLRVLRVLRPLKTIRRVPKLKAVFDCVVSS---LKNVGIILVVYILFQFIFSVVAVQLFQGKF------------FYCTDQSKY--TVEQCQGYYFS--YEEGPIPVVKPRVWSSRDFHYDNVISAMLTLFTVTTGEGWPGILKNSMDATE 1019
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. TrEMBL
Match: A0A0Q9WQ19 (Uncharacterized protein, isoform X OS=Drosophila virilis OX=7244 GN=Dvir\GJ18738 PE=3 SV=1) HSP 1 Score: 1348.95 bits (3490), Expect = 0.000e+0 Identity = 823/1527 (53.90%), Postives = 1066/1527 (69.81%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLF---DATQQACYPQSNTS-------CNAGFT-CKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF-NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP+GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P ++ C ++T CN+ + C WDGP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L G +++ D ++ K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK E C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H N + E+ SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E ++S + N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+ + DTQ+N++NNF++F G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WRN
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPEGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQMGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNSTEKAGGSFVCNSTTSMCLEKWDGPNSGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSTFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPTFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAKK----GMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQGDGVHLENGDGTVATSKGKS---KKKEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSEECQGSYFKYEEDELLPKQEMRVWKPRSFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYH----------NQSEMYEK--------SLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNS--INVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKYDPDTQLNRHNNFQSFSGGIMLLFRCATGEAWPNIMLACLKGKACDDDAEKGAG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGRIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADIELRETITNIWP-LQAKKMLNLLVPPIDQLNKGK--LSVGKIYAGFLILESWRN 1495 HSP 2 Score: 157.532 bits (397), Expect = 1.932e-34 Identity = 163/679 (24.01%), Postives = 302/679 (44.48%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQS----FGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q + ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ S GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG D + + + NF +F I+ +F TGE W +M ++ + K + S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSEECQGSYFKYEEDELLPKQEMRVWKPRSF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQSEMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGHDDSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKYDPDTQLNRHN-NFQSFSGGIMLLFRCATGEAWPNIMLACLKGKACDDDAEKGAGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1337
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. TrEMBL
Match: A0A0Q9WPT4 (Uncharacterized protein, isoform AC OS=Drosophila virilis OX=7244 GN=Dvir\GJ18738 PE=3 SV=1) HSP 1 Score: 1345.87 bits (3482), Expect = 0.000e+0 Identity = 823/1527 (53.90%), Postives = 1063/1527 (69.61%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLF---DATQQACYPQSNTS-------CNAGFT-CKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHE--------------MRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTF-NDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP----LNKASYPLTVGKIYAGMLIMKNWRN 4657
SLFI +E+N IR+ + +IE FEY V+LTIIANCV LALE HLP+GD+T + K+ E FL IF EA LKI+ALG ++ K++YL+++WNI+DF VV G +T Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H TC+ + P ++ C ++T CN+ + C WDGP G+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIV+GSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EEK +M+ R++ +RK + + ++ + + + ++K + R ++H +KTQ FYW VI LV LNT CVAVEHYGQP FL FL+YAE++FL +FM+E+FIK++ G R YF S FN FD +V++ S+ E I+ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++++ ++ + +E L G +++ D ++ K K++ KE + E P K +LPYSS+FI P N IRR H+V NL +FD FIM+VI SS+ALAAEDPV NS RN++L +FD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VI VQLF+GKFFYC D SK E C+G ++ + D++ + + R+W+ F+YDN+ +M+TLF V TGEGWP VL++S+ ATY + GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP N+N+ YKVW +VVS PFEY IM++I +++ L++K H N + E+ SLKY+N+ FT +F+ E +LK+I + K FFKDPWNIFD +TVLGS+ D E S N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNI+ + DTQ+N++NNF++F G++LLFRCATGE+W IM+ GK C G + CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD++ RV F +TLFALIRE+L IKM + E+D D ELR + +WP L + ++ +P LNK L+VGKIYAG LI+++WRN
Sbjct: 17 SLFILTEDNPIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPEGDKTVLAQKLEKTEAYFLCIFCVEASLKILALGLVLHKHSYLRNIWNIMDFFVVVTGFMTQYPQMGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHKTCYSLEDPNKLVKEGESETPCNTDNSTEKAGGSFVCNSTTSMCLEKWDGPNSGITSFDNIGFAMLTVFQCITMEGWTSILYWTNDALGSTFNWIYFVPLIVIGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLERELNGYVEWICKAEEVILAEERTTEEEKMHIMEARRRNAA--KRKKLKSLGKSKSTDTEEEEAEEDYGDDGYLKTRSKPQGSCTGFWRAEKRFRFWIRHTVKTQWFYWFVIVLVFLNTVCVAVEHYGQPTFLTEFLYYAEFIFLGLFMSEMFIKMYALGPRIYFESSFNRFDCVVISGSIFEVIWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPGGTPETNFNTFPIALLTVFQILTGEDWNEVMYQGIISQGGAKK----GMIYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQVEEDKEKQLQELEKEMEALQGDGVHLENGDGTVATSKGKS---KKKEEEKKEEEEVTEGP--------KPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNYFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFFYCTDESKHTSEECQGSYFKYEEDELLPKQEMRVWKPRSFHYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKNRNTFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYH----------NQSEMYEK--------SLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSI-----NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKYDPDTQLNRHNNFQSFSGGIMLLFRCATGEAWPNIMLACLKGKACDDDAEKGAG-------EYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGRIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDDELRVQFTTTLFALIRENLSIKMRAPEEMDQADIELRETITNIWP-LQAKKMLNLLVPPIDQLNKGK--LSVGKIYAGFLILESWRN 1492 HSP 2 Score: 157.532 bits (397), Expect = 1.617e-34 Identity = 162/676 (23.96%), Postives = 302/676 (44.67%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ-SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQM------KTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+FI S N IRR ++ +F++ +++ I + ++LA E + + R + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IF++IG++++ G F + TD Q Y + W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M ++ N KY V + + PF + ++ L++ NT + ++++ Q + ++ L Y F +F E +KI GFG++++F+ +N+FD I V S+V+A++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG D + + + NF +F I+ +F TGE W +M ++ + K + S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 739 GPKPMLPYSSMFILSPTNPIRRGAHWVVNLPYFDFFIMVVISMSSIALAAEDPVRENSRRNKILNY--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMSGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVVNILIVYILFQFIFSVIGVQLFNGKFF---YCTDESKHTSEECQGSYFKYEEDELLPKQEMRVWKPRSF---HYDNVAAAMLTLFAVQTGEGWPQVLQHSMAATYEDRGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPKN--------------------RNTFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQSEMYEKSLKYINMGFTGMFSVETVLKIIGFGVKNFFKDPWNIFDLITVLGSIVDALWMEFGSNSINVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKYDPDTQLNRHN-NFQSFSGGIMLLFRCATGEAWPNIMLACLKGKACDDDAEKGAGEYCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1334
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. TrEMBL
Match: A0A182FNU6 (EF-hand domain-containing protein OS=Anopheles albimanus OX=7167 PE=3 SV=1) HSP 1 Score: 1345.1 bits (3480), Expect = 0.000e+0 Identity = 833/1529 (54.48%), Postives = 1059/1529 (69.26%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDS----------PFLFDATQQACYPQSNTSCNAG-FTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMK-------------NRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXX----XXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY-------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFN--DQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKA--SYPLTVGKIYAGMLIMKNWRN 4657
SLFI SE+NIIR+ + +IE FEY V+LTIIANCV LALE HLP GD+T + K+ E FLGIF EA LKI+ALGF++ K++YL+++WNI+DF VV G IT+ Q VDLRT RAIRVLRP KLVSG+PSLQVVL SIIKAMAPLLQIGLLVLFA++IFAIIGLE Y G H +C+ + P +A P CN+ TC W+GP FG+ +FDNIG+AMLTVFQCITMEGWT +LY TN A S +N +YFV LIVLGSFFMLNLVLGVLSGEFAKERE+VE R+ FLKLR Q+ E L+GY+ W+ AEE+I E + EE+ +M+ N K+K + DE + W + R ++H +KTQ FYW VI LV NT CVAVEHYGQP +L +FL+YAEYVFL +FM E++IK++ G R YF S FN FD +V++ S+ E ++ + SFG S LR +RLLR+ K+T+YWSSLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG FN G P NF+TFP A++TVF ILTGE+WNEVMY GI SQ MIYS YF+++V+FGNYTLLNVFLAIAVDNLANA EL+AAEEE+ ++ + +++ + + GS +++ S PK KK+ + E P K +LPYSS+F+ P N IR H+V NLR+FD FIM+VI SS+ALAAEDPV +S RN++L FFD AFT VF +EM+LK++D+G++LHPGSY+R+ WNIMD +VVI A SF + +NL+TIKSLRV+RVLRPLKTI RVPKLKAVF+ +++S+KNV NILIV+ILFQFIF VIAVQLF+GKFFYC D SK E CKG+F+ ++ DQ+ + + R W+ F+YDN+ +M+TLF V TGEGWP VL+NS+ ATY ++GPI+N++ E++IFYIV+FIVFPFFFVNIFVALII+TFQEQGE++ D E+DKN+ CIDF I +P RYMP +NS YKVW +VVS PFEY IM++I +++ L++K H + E F SLKYLN+ FT +F+ E ILK+I + K FFKDPWNIFDF+TV+GS+ D + E+ E S N+GFLRLFR RLIK+L +GY+IRILLWTF+QSFKALP+VCLLI +LFFIY IIGMQ+FGNIEL+ ++ I ++NNFR+F+ GL+LLFRCATGESW IM+ G+ C + ++ CGS +Y YF++FI +FLMLNLFVAVIMDNFDYLTRD SILG HHLDE+VR+WAE+DP TG +++ +MY M+K + PP+GFG CP R A KLIRMNM LD + +V F +TLFALIRE+L IKM + E+D D ELR + +WP L + +D +PL++ + LTVGKIYAG+LI+++WRN
Sbjct: 82 SLFILSEDNIIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHGDKTILAQKLEKTEAYFLGIFCVEASLKILALGFVMHKHSYLRNIWNIMDFFVVVTGFITLFPQKGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHRSCYSLEDITQIVKEGEFPTPCNADNDTIAPTGAYVCNSSDSTCVEQWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWTNDALGSTFNWIYFVPLIVLGSFFMLNLVLGVLSGEFAKEREKVENRQEFLKLRRQQQLEKELNGYVEWICKAEEVILAEERTTEEERLHIMEARRRAAAKRKKLKNLGKSKSTDTEEDDPDEDCGDDGYLKAKVKTQGKCKGFW-RAEKRFRFWIRHTVKTQWFYWFVIVLVFFNTVCVAVEHYGQPNWLTQFLYYAEYVFLGLFMMEMWIKMYALGPRIYFESSFNRFDCVVISGSIFEVVWSEVK-GGSFGLSVLRALRLLRIFKVTKYWSSLRNLVISLLNSMRSIISLLFLLFLFILIFALLGMQLFGGQFNLPDGTPPTNFNTFPIALLTVFQILTGEDWNEVMYQGIESQ----GGHKRGMIYSLYFIILVLFGNYTLLNVFLAIAVDNLANAQELTAAEEEQLEENKEKQQMELDKEMEALQLQNDGSPPRVEVSSPSPTRGNGSGGGPKAKKKDDDKEEDDDEIPDGP-----KPMLPYSSMFVLSPTNPIRCAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVEEDSPRNKILNFFDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKHTSEECKGHFFVYDGADQLP-RREAREWKTQSFHYDNVATAMLTLFAVQTGEGWPQVLQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVFPFFFVNIFVALIIITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPNKRNSFKYKVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGKE------------------FEKSLKYLNMGFTGMFSVETILKIIGFGVKNFFKDPWNIFDFITVIGSIIDALVLEL-GENSF----NVGFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIELDPESSITRHNNFRSFVQGLMLLFRCATGESWPNIMLACLKGRPCDPRA--------NKPNETCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMDNFDYLTRDSSILGAHHLDEFVRIWAEYDPNATGKIHYTEMYDMLKNMDPPLGFGNKCPNRLAYKKLIRMNMPLDAEGKVGFTTTLFALIRENLNIKMRTAEEMDQADMELRHTISHIWP-LQAKKMLDLLVPLSEELNAGKLTVGKIYAGLLILESWRN 1566 HSP 2 Score: 154.836 bits (390), Expect = 1.007e-33 Identity = 161/685 (23.50%), Postives = 303/685 (44.23%), Query Frame = 2
Query: 167 GQKPN-PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ-------ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIF--------HNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS----------QWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAE-EKKELMKNRQKTKEW------LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEA-IFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTL-----NFDTFPNAIITVFVILTGENWNEVMYNGIRS-----QMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
G KP P S+F+ S N IR + ++ R+F++ +++ I + ++LA E + + + + F G+FT E +LKI+ LG I+ +YL+ WNI+D +VV ++ + +++ R +RVLRP K + VP L+ V ++ ++ ++ I ++ + IFA+I ++++ G F H + F++D Q ++ W F ++DN+ AMLT+F T EGW VL + AT + ++ Y V IV FF +N+ + ++ +I F+ + AE + E+ KN++ ++ LER M N KY V + + PF + ++ L++ NT + ++++ Q K ++ L Y F +F E +KI GFG++++F+ +N+FDFI V S+++A + ++ + GF L L L Y ++R L+++ + S +++ + LL+ + FI ++GMQ+FG N E + NF +F ++ +F TGE+W +M ++ + N S + +YF+ + F ++ +LN+F+A+ +D
Sbjct: 814 GPKPMLPYSSMFVLSPTNPIRCAAHWVVNLRYFDFFIMVVISLSSIALAAEDPVEEDSPRNKILNF--FDYAFTGVFTIEMLLKIVDLGVILHPGSYLREFWNIMDAVVVICAAVSFGFDMTGSSAGQNLSTIKSLRVLRVLRPLKTIKRVPKLKAVFDCVVNSLKNVINILIVYILFQFIFAVIAVQLFNGKFFYCTDDSKHTSEECKGHFFVYDGADQLPRREARE-----------WKTQSF---HYDNVATAMLTLFAVQTGEGWPQVLQNSMAATYEDKGPIQNFRIEMSIFYIVYFIVF-PFFFVNIFVALI------------------------------------------IITFQEQGEAELQDGEIDKNQKSCIDFTIGARPLERYMPNK--------------------RNSFKY------KVWRIVVSTPFEYFIMMLIVFNTLLLMMKYHNQGKEFEKSLKYLNMGFTGMFSVETILKIIGFGVKNFFKDPWNIFDFITVIGSIIDALVLELGENSFNVGFLRLFRAARLIKLLRQGY--TIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFG---NIELDPESSITRHNNFRSFVQGLMLLFRCATGESWPNIMLACLKGRPCDPRANKPNETCGSTLAYAYFVSFIFFCSFLMLNLFVAVIMD 1408 HSP 3 Score: 102.449 bits (254), Expect = 8.666e-18 Identity = 76/266 (28.57%), Postives = 134/266 (50.38%), Query Frame = 2
Query: 2357 SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNT-IKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF----FYCNDLSKRIKE---------------TCKGYFYTFNDQMGIQAKKRIWQRNYF---NYDNIVNSMITLFTVTTGEGWPDVL 3079
+SLFI N IR+ F+ F+ +++ I A+ + LA E+ P ++ + LE + F +F +E LK++ +G V+H SY+R+ WNIMD VV+ + F K +++LR IRVLRPLK ++ +P L+ V ++II ++ + I ++ + IF +I ++ +SG + D+++ +KE Y +D ++ W+ F ++DNI +M+T+F T EGW +L
Sbjct: 81 TSLFILSEDNIIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEHLPHGDKTILAQKLEKTEAYFLGIFCVEASLKILALGFVMHKHSYLRNIWNIMDFFVVVTGFITLFPQKGPEVDLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHRSCYSLEDITQIVKEGEFPTPCNADNDTIAPTGAYVCNSSDSTCVEQ----WEGPNFGITSFDNIGFAMLTVFQCITMEGWTAIL 342 HSP 4 Score: 66.6254 bits (161), Expect = 6.535e-7 Identity = 79/344 (22.97%), Postives = 136/344 (39.53%), Query Frame = 2
Query: 3311 TSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNI------ELNDDTQI----------NQNNN------------------------------FRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
TS ++ + N + ++ PPFEY +++ I + + L ++ H LP + L+ F IF E LK++A F + + ++ WNI DF V+ +F + E+ +L LR RV+R +K++ S++++L + I++ L + LL+ I+ IIG++ + L D TQI N +N+ F N +L +F+C T E W I+ T D L GS F++ YF+ I++ +F MLNL + V+ F
Sbjct: 81 TSLFILSEDNIIRKYTRFIIEWPPFEYAVLLTIIANCVVLALEEH--LP-------------HGDKTILAQKLEKTEAYFLGIFCVEASLKILALGFVMHKHSYLRNIWNIMDFFVVVTGFITLF------PQKGPEV-DLRTLRAIRVLRPLKLVSGIPSLQVVLKSIIKAMAPLLQIGLLVLFAIVIFAIIGLEFYSGALHRSCYSLEDITQIVKEGEFPTPCNADNDTIAPTGAYVCNSSDSTCVEQWEGPNFGITSFDNIGFAMLTVFQCITMEGWTAILYWT--------------------NDAL-GSTFNWIYFVPLIVLGSFFMLNLVLGVLSGEF 381
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Cavefish
Match: cacna1da (calcium channel, voltage-dependent, L type, alpha 1D subunit, a [Source:ZFIN;Acc:ZDB-GENE-030616-135]) HSP 1 Score: 1025.39 bits (2650), Expect = 0.000e+0 Identity = 645/1507 (42.80%), Postives = 915/1507 (60.72%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT---VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY--PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V + D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ S + + C T G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ ++ + K+ +T+ + ++ W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S+S MI YF+++ I GNY LLNVFLAIAVD N+ D+ + + P P I E ++ ++KI P S+ FIF N IR CH + N F I++ I SS +LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ CND +K E CKG + + D Q +KR W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS T F + LN+AFT +FT E +LK+IA++ + +F D WN+FD + V+GS+ D+ LSE+ + E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQIFG + + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ +GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 66 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVGSEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDVLYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPDNEEEEEDSKRNPSVPASETESVNTENEKGEDEKATCCGPLCQKISKSKFSRRWRRWNRFCRRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPMWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNVVMYDGIMAYGGPSSS---GMIVCIYFIILFICGNYILLNVFLAIAVD----------------------------------------------------NLADEEDEPEVPSGPRPAISE------LVKKEKITPIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIILGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYILYKDGDVNQPTLQKRHWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNKENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG-------QSQT-----------FNYVMDILNMAFTAVFTVEMVLKLIAFKPRGYFGDAWNVFDALVVIGSIVDIVLSEIDNTEDSARI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQIFGKVAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACMAGKLCDPESDYNPG-----EEMTCGSSFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1481 HSP 2 Score: 106.301 bits (264), Expect = 2.259e-22 Identity = 77/269 (28.62%), Postives = 132/269 (49.07%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N IR +C LI F L+++ I+ + SLA E + I+ + + F IFT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S + + C + N K +W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 734 PIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNII--LGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKS------SPEECKGTYILYKDGDVNQPTLQKRHWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 988 HSP 3 Score: 72.7886 bits (177), Expect = 4.109e-12 Identity = 81/342 (23.68%), Postives = 143/342 (41.81%), Query Frame = 2
Query: 3293 AINTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-----------GNIELNDD------------------TQINQN--------NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ NT+P N N + LV PF+ I+I I + + L + + PE SN ST +D L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ + + ++ LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G+ +++D T+ + NF NFM +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 61 SANTRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPED--DSN-------STNHD----LETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVIP-----LAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVGSEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 361
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Cavefish
Match: cacna1db (calcium channel, voltage-dependent, L type, alpha 1D subunit, b [Source:ZFIN;Acc:ZDB-GENE-040525-3]) HSP 1 Score: 1022.31 bits (2642), Expect = 0.000e+0 Identity = 654/1491 (43.86%), Postives = 923/1491 (61.90%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSN-TSCNA-GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKK--------ELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG-KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPP-AEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYF--YTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIP---------NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVW 4540
P R+LF + NN IRR+C L+E + F+ ++L+I ANCV+LA+ V P+ D + +E FL IFT E LKIIA G ++ +N+Y+++ WN+LDF++V +GL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ S L D C + C G CK W GP G+ NFDN +AMLTVFQCITMEGWT+VLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ +++ +E K E + K R IC +W ++ R + +K+ FYW VI LV LNT ++ EHY QP +L A V L++F E+ IK++ G++ YF S FN FD VV +VE I + G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQLFGG FNF++ FD FP A++TVF ILTGE+WN VMY+GI + S+S M+ YF+++ I GNY LLNVFLAIAVDNLA+A L+ A++EE ++ + R++++ + R S +E P + Q + E + + PP EG S+ FIF N IR CH + N + F I++ I SS++LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K E CKG + Y D Q ++RIW + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L I+ H + + F ++ LN+ FT +FT E ILK++A++ + +F D WN+FD + V+GS+ D+ LS++ ES T ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D T IN+NNNF+ F +LLLFRCATGE+W IM+ GK C + G ++K CGS+F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W
Sbjct: 89 PPRALFCLTLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYVPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNSYVRNGWNMLDFVIVIVGLFSVVLEFLTKEGDSSQSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMNGSRTLADEESAPCSLSGHGRHCTVNGTLCKDGWQGPNNGITNFDNFLFAMLTVFQCITMEGWTEVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEGDQEGKRNPSMPTSETESVNTDNPNGEDDKARCCGPIC----------------RSWRRWNRFCRRKCRAAVKSTTFYWLVILLVFLNTLTISSEHYNQPDWLTEVQDVANKVLLAMFTCEMLIKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETVTKRSTFDNFPQALLTVFQILTGEDWNTVMYDGIMAYGGPSSS---GMVVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEEEQNKRRKKQSIKQADRNSHEVHDVAHHRHGSEDDEDEPEVPPGPRPQRLSELTIKEKTPPIPEG----------SAFFIFSNTNPIRVSCHRLINHQIFTNLILVFIMLSSVSLAAEDPIRSYSARNIILGYFDYAFTAIFTVEILLKMTAFGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSSPEECKGTYILYNIGDNALPQVRERIWFNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENMGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNRYQYKFWYVVNSAGFEYVMFVLIILNTLCLAIQHHGQ------------------SHLFNYAMDILNMVFTGVFTVEMILKLVAFKPRGYFGDAWNVFDALVVIGSVVDIILSQVDVIESWTATAGNTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTSINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDSESDYNPG-----EEKTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIW 1523 HSP 2 Score: 109.383 bits (272), Expect = 3.076e-23 Identity = 82/266 (30.83%), Postives = 134/266 (50.38%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPY-WDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N IR SC LI + F L+++ I+ + VSLA E + I+ + + F IFT E +LK+ A G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ TD + Y N NA + W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 806 PIPEGSAFFIFSNTNPIRVSCHRLINHQIFTNLILVFIMLSSVSLAAEDPIRSYSARNII--LGYFDYAFTAIFTVEILLKMTAFGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRC---TDEAKSSPEECKGTYILYNIGDNALPQVRERIW---FNSDFNFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1060
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Cavefish
Match: cacna1da (calcium channel, voltage-dependent, L type, alpha 1D subunit, a [Source:ZFIN;Acc:ZDB-GENE-030616-135]) HSP 1 Score: 1016.91 bits (2628), Expect = 0.000e+0 Identity = 654/1554 (42.08%), Postives = 927/1554 (59.65%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT---------------------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFE------NKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN-------------------WIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ + + A T G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ +K+N +LM ++K + + + E+ + K E W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S+S MI YF+++ I GNY LLNVFLAIAVD N+ D+ + + P P I E ++ ++KI P S+ FIF N IR CH + N F I++ I SS +LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ CND +K E CKG + + D Q +KR W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS T F + LN+AFT +FT E +LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ + E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQIFG + + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ +GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 56 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKDGGQEDEGVEIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDVLYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPDNEEEEEDSKRNRVTLADLMDKKKKGFSFFSQSSETHASVPASETESVNTENEKGEDEKATCCGPLCQKISKSKFSRRWRRWNRFCRRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPMWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNVVMYDGIMAYGGPSSS---GMIVCIYFIILFICGNYILLNVFLAIAVD----------------------------------------------------NLADEEDEPEVPSGPRPAISE------LVKKEKITPIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIILGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYILYKDGDVNQPTLQKRHWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNKENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG-------QSQT-----------FNYVMDILNMAFTAVFTVEMVLKLIAFKPRHYFTDAWNTFDALIVVGSVVDIAITEVNNTEDSARI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQIFGKVAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACMAGKLCDPESDYNPG-----EEMTCGSSFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1518 HSP 2 Score: 106.686 bits (265), Expect = 1.768e-22 Identity = 77/269 (28.62%), Postives = 132/269 (49.07%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N IR +C LI F L+++ I+ + SLA E + I+ + + F IFT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S + + C + N K +W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 771 PIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNII--LGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKS------SPEECKGTYILYKDGDVNQPTLQKRHWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1025 HSP 3 Score: 77.0258 bits (188), Expect = 1.817e-13 Identity = 87/359 (24.23%), Postives = 146/359 (40.67%), Query Frame = 2
Query: 3293 AINTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLS----EMKSEESTTEIP---------------NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF---------------------------GNIELNDDTQINQN--------NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ NT+P N N + LV PF+ I+I I + + L + + PE SN ST +D L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + E+ EIP ++ LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G + T+ + NF NFM +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 51 SANTRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPED--DSN-------STNHD----LETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKDGGQEDEGVEIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 373
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Cavefish
Match: cacna1da (calcium channel, voltage-dependent, L type, alpha 1D subunit, a [Source:ZFIN;Acc:ZDB-GENE-030616-135]) HSP 1 Score: 1010.75 bits (2612), Expect = 0.000e+0 Identity = 648/1539 (42.11%), Postives = 916/1539 (59.52%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT---------------------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMK-------SEESTTEIP---NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ + + A T G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ ++ + K+ +T+ + ++ W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S+S MI YF+++ I GNY LLNVFLAIAVD N+ D+ + + P P I E ++ ++KI P S+ FIF N IR CH + N F I++ I SS +LAAEDP+ S RN +L + D FTS+F E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ CND +K E CKG + + D Q +KR W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS T F + LN+AFT +FT E +LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ E TE ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQIFG + + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ +GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 56 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKDGGQEDEGVEIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDVLYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPDNEEEEEDSKRNPSVPASETESVNTENEKGEDEKATCCGPLCQKISKSKFSRRWRRWNRFCRRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPMWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNVVMYDGIMAYGGPSSS---GMIVCIYFIILFICGNYILLNVFLAIAVD----------------------------------------------------NLADEEDEPEVPSGPRPAISE------LVKKEKITPIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIVLGYADYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYILYKDGDVNQPTLQKRHWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNKENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG-------QSQT-----------FNYVMDILNMAFTAVFTVEMVLKLIAFKPRHYFTDAWNTFDALIVVGSVVDIAITEVNPTEAPQVDESGNTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQIFGKVAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACMAGKLCDPESDYNPG-----EEMTCGSSFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1504 HSP 2 Score: 107.457 bits (267), Expect = 1.281e-22 Identity = 78/269 (29.00%), Postives = 133/269 (49.44%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N IR +C LI F L+++ I+ + SLA E + IV A + +F +FT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S + + C + N K +W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 746 PIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIVLGYA--DYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKS------SPEECKGTYILYKDGDVNQPTLQKRHWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1000 HSP 3 Score: 77.411 bits (189), Expect = 1.446e-13 Identity = 87/359 (24.23%), Postives = 146/359 (40.67%), Query Frame = 2
Query: 3293 AINTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLS----EMKSEESTTEIP---------------NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF---------------------------GNIELNDDTQINQN--------NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ NT+P N N + LV PF+ I+I I + + L + + PE SN ST +D L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + E+ EIP ++ LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G + T+ + NF NFM +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 51 SANTRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPED--DSN-------STNHD----LETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKDGGQEDEGVEIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 373
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Cavefish
Match: cacna1da (calcium channel, voltage-dependent, L type, alpha 1D subunit, a [Source:ZFIN;Acc:ZDB-GENE-030616-135]) HSP 1 Score: 1010.75 bits (2612), Expect = 0.000e+0 Identity = 651/1541 (42.25%), Postives = 920/1541 (59.70%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKM---------AGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT---------------------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY--PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEW-LERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ S + + C T G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ ++ + K+ +T+ E + +DE W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S+S MI YF+++ I GNY LLNVFLAIAVD N+ D+ + + P P I E ++ ++KI P S+ FIF N IR CH + N F I++ I SS +LAAEDP+ S RN +L + D FTS+F E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ CND +K E CKG + + D Q +KR W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS T F + LN+AFT +FT E +LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ + E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQIFG + + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ +GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 56 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSTNHDLNCPSPQSIIETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKDGGQEDEGVEIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVGSEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDVLYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPDNEEEEEDSKRNPSVPASETESVNTENEKGEDE----------KATCCGPLCRRWRRWNRFCRRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPMWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNVVMYDGIMAYGGPSSS---GMIVCIYFIILFICGNYILLNVFLAIAVD----------------------------------------------------NLADEEDEPEVPSGPRPAISE------LVKKEKITPIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIVLGYADYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYILYKDGDVNQPTLQKRHWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNKENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG-------QSQT-----------FNYVMDILNMAFTAVFTVEMVLKLIAFKPRHYFTDAWNTFDALIVVGSVVDIAITEVNNTEDSARI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQIFGKVAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACMAGKLCDPESDYNPG-----EEMTCGSSFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1495 HSP 2 Score: 107.071 bits (266), Expect = 1.586e-22 Identity = 78/269 (29.00%), Postives = 133/269 (49.44%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N IR +C LI F L+++ I+ + SLA E + IV A + +F +FT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S + + C + N K +W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 748 PIPEGSAFFIFSNTNPIRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIVLGYA--DYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKS------SPEECKGTYILYKDGDVNQPTLQKRHWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1002 HSP 3 Score: 78.9518 bits (193), Expect = 4.973e-14 Identity = 86/361 (23.82%), Postives = 149/361 (41.27%), Query Frame = 2
Query: 3293 AINTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLS----EMKSEESTTEIP---------------NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-----------GNIELNDD------------------TQINQN--------NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ NT+P N N + LV PF+ I+I I + + L + + PE S L S Q+ ++ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + E+ EIP ++ LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G+ +++D T+ + NF NFM +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 51 SANTRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPEDDSNSTNHDLNCPSPQS----IIETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKDGGQEDEGVEIPLAAATHAHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCFLVGSEMISEDEPAPCAVNGHGRTCTINGTECREGWHGPNGGITNFDNFMFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGMELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 384
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005302.1 (pep scaffold:Pmarinus_7.0:GL483978:134:5921:-1 gene:ENSPMAG00000004828.1 transcript:ENSPMAT00000005302.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 303.138 bits (775), Expect = 3.633e-93 Identity = 148/289 (51.21%), Postives = 206/289 (71.28%), Query Frame = 2
Query: 3716 NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLP 4582
++ F RLFRV+RL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D +QIN+NNNF+ F +LLLFRCATGE+W IM+ G+RC + G ++ CGS F+ YF++F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R SL+IK + ++ ++ELR + K+W N + +D +P
Sbjct: 18 SITFFRLFRVLRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMQDGSQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGRRCDPDADASVG-----EEFSCGSSFAIFYFVSFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHVDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTSLKIK--TDGNLEQANEELRAVIKKIWKRTNMK-LLDQVVP 298
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010500.1 (pep scaffold:Pmarinus_7.0:GL477503:21958:35427:1 gene:ENSPMAG00000009511.1 transcript:ENSPMAT00000010500.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 278.87 bits (712), Expect = 2.456e-85 Identity = 134/269 (49.81%), Postives = 187/269 (69.52%), Query Frame = 2
Query: 3812 SFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSV-AEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVG 4615
S +ALP+VCLLI +LFFIY I+GMQ+FGN++L+D+ I ++NNF+ F L+LLFR ATGE+W IM+ S K C +L + +CGSDF+Y YF++FI + +FLMLNLFVAVIMDNF+YLTRD SILGPHHLDE+VR+W ++DP TG + + MY++++ + PP+G GK CP R A +L+RM+M + V+F +TL ALIR +L IK+ A+ D ELR M+ +WP+L+ Q +D +P ++A LTVG
Sbjct: 1 SLQALPYVCLLIAMLFFIYAIVGMQVFGNVKLDDEGGITEHNNFQTFFQALMLLFRSATGEAWQEIMLSCLSKKPCEQHSLN--------GENVCGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRIWGDYDPAATGRITYTKMYELLRHMSPPLGLGKKCPVRIAYKRLVRMDMPIAEDNTVHFTTTLMALIRTALDIKIAKAGADKFECDSELRKEMMAIWPTLS-QKTLDILVPPHRA-VDLTVG 259
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008630.1 (pep scaffold:Pmarinus_7.0:GL481395:151307:166184:-1 gene:ENSPMAG00000007818.1 transcript:ENSPMAT00000008630.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 247.669 bits (631), Expect = 1.064e-70 Identity = 207/495 (41.82%), Postives = 293/495 (59.19%), Query Frame = 2
Query: 1382 IKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVF-VILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQ--------DIDDSIIEELNMYDKPKDKKQPIK-----------------ESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFF--YSKNLNTIKSLRVIRVLRPLKTINRVPKLK 2770
+K+ FYW VI LV LNT +A EHY Q ++L A V L++F E+ +KI+ G++ YF S FN FD VV +VE I + G S LRC+RLLR+ K TRYW+SL NLV SLL+SMRSI SLLLLL LFI I +LLGMQLFGG FNF++ + +FD FP A++TVF ILTGE+WN VMY+GIR+ S M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A+ E +++E ++ R G+ + +DD E+ DK KD P + E P+ + +++K++P S+ F+F N R +CH + N F I++ I SS++LAAEDP+ S RN +L +FD AFT++F +E++LK+ G +H GS+ R +N++D++VV +++ SF +S ++ +K LRV+RVLRPL+ INR LK
Sbjct: 77 VKSTAFYWMVIVLVFLNTLTIASEHYDQMQWLTHVQDTANKVLLALFTLEMLVKIYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELKVMSPLGISVLRCVRLLRIFKATRYWNSLSNLVASLLNSMRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQVRRSSFDNFPTALLTVFQQILTGEDWNSVMYDGIRAYGGPSFP---GMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQREAEEEKERKKIARITPRERVWNGNPGLVSVCVSVWKIPLDDPDGED----DKEKDPYPPSEYPGWRRHDTGDDDESEPEVPMGPRPRRLSELQIKEKVVPMPESSAFFLFSHTNPFRILCHHIINHTIFTNLILVFIMLSSVSLAAEDPIRSTSFRNEILGYFDYAFTTIFTIEIMLKMTAYGAFVHKGSFCRSYFNLLDLLVVGVSLISFGIQWSSAISVVKILRVLRVLRPLRAINRAKGLK 564 HSP 2 Score: 68.1662 bits (165), Expect = 1.167e-11 Identity = 47/147 (31.97%), Postives = 77/147 (52.38%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV--DLRTFRAIRVLRPFKLVSGVPSLQ 607
P P+ S F+FS N R C +I F L+++ I+ + VSLA E P +F + + F IFT E +LK+ A G V K ++ +S +N+LD +VV + LI+ +Q + ++ R +RVLRP + ++ L+
Sbjct: 420 PMPESSAFFLFSHTNPFRILCHHIINHTIFTNLILVFIMLSSVSLAAED--PIRSTSFRNEILGYFDYAFTTIFTIEIMLKMTAYGAFVHKGSFCRSYFNLLDLLVVGVSLISFGIQWSSAISVVKILRVLRVLRPLRAINRAKGLK 564 HSP 3 Score: 64.6994 bits (156), Expect = 1.506e-10 Identity = 70/282 (24.82%), Postives = 132/282 (46.81%), Query Frame = 2
Query: 3353 KVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLF-RCATGESWHLIMIDTESGKRCACGNLTTGG-ICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
K V S F +++++++ L+++T+ + + ++ Q T N L+L FT E ++K+ + + +F +N FD V G + + L E+K + LR R++R+ K S+ L+ + + S +++ + LL+ L I+ ++GMQ+FG D+TQ+ + ++F NF LL +F + TGE W+ +M D G R G G +C+ YFI + +++LN+F+A+ +DN
Sbjct: 72 KCRAAVKSTAFYWMVIVLVFLNTLTIASEHYDQM--------QWLTHVQDTANKVLLAL----------FTLEMLVKIYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELKVMSPL----GISVLRCVRLLRIFKATRYWNSLSNLVASLLNSMRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQV-RRSSFDNFPTALLTVFQQILTGEDWNSVMYD---GIRAYGGPSFPGMLVCI--------------YFIILFICGNYILLNVFLAIAVDNL 313
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008632.1 (pep scaffold:Pmarinus_7.0:GL481395:151307:166184:-1 gene:ENSPMAG00000007818.1 transcript:ENSPMAT00000008632.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 246.899 bits (629), Expect = 1.077e-70 Identity = 207/495 (41.82%), Postives = 293/495 (59.19%), Query Frame = 2
Query: 1382 IKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGK-PTLNFDTFPNAIITVF-VILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQ--------DIDDSIIEELNMYDKPKDKKQPIK-----------------ESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFF--YSKNLNTIKSLRVIRVLRPLKTINRVPKLK 2770
+K+ FYW VI LV LNT +A EHY Q ++L A V L++F E+ +KI+ G++ YF S FN FD VV +VE I + G S LRC+RLLR+ K TRYW+SL NLV SLL+SMRSI SLLLLL LFI I +LLGMQLFGG FNF++ + +FD FP A++TVF ILTGE+WN VMY+GIR+ S M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A+ E +++E ++ R G+ + +DD E+ DK KD P + E P+ + +++K++P S+ F+F N R +CH + N F I++ I SS++LAAEDP+ S RN +L +FD AFT++F +E++LK+ G +H GS+ R +N++D++VV +++ SF +S ++ +K LRV+RVLRPL+ INR LK
Sbjct: 57 VKSTAFYWMVIVLVFLNTLTIASEHYDQMQWLTHVQDTANKVLLALFTLEMLVKIYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELKVMSPLGISVLRCVRLLRIFKATRYWNSLSNLVASLLNSMRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQVRRSSFDNFPTALLTVFQQILTGEDWNSVMYDGIRAYGGPSFP---GMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQREAEEEKERKKIARITPRERVWNGNPGLVSVCVSVWKIPLDDPDGED----DKEKDPYPPSEYPGWRRHDTGDDDESEPEVPMGPRPRRLSELQIKEKVVPMPESSAFFLFSHTNPFRILCHHIINHTIFTNLILVFIMLSSVSLAAEDPIRSTSFRNEILGYFDYAFTTIFTIEIMLKMTAYGAFVHKGSFCRSYFNLLDLLVVGVSLISFGIQWSSAISVVKILRVLRVLRPLRAINRAKGLK 544 HSP 2 Score: 68.5514 bits (166), Expect = 9.138e-12 Identity = 47/147 (31.97%), Postives = 77/147 (52.38%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTV--DLRTFRAIRVLRPFKLVSGVPSLQ 607
P P+ S F+FS N R C +I F L+++ I+ + VSLA E P +F + + F IFT E +LK+ A G V K ++ +S +N+LD +VV + LI+ +Q + ++ R +RVLRP + ++ L+
Sbjct: 400 PMPESSAFFLFSHTNPFRILCHHIINHTIFTNLILVFIMLSSVSLAAED--PIRSTSFRNEILGYFDYAFTTIFTIEIMLKMTAYGAFVHKGSFCRSYFNLLDLLVVGVSLISFGIQWSSAISVVKILRVLRVLRPLRAINRAKGLK 544 HSP 3 Score: 65.0846 bits (157), Expect = 1.024e-10 Identity = 70/282 (24.82%), Postives = 132/282 (46.81%), Query Frame = 2
Query: 3353 KVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLF-RCATGESWHLIMIDTESGKRCACGNLTTGG-ICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
K V S F +++++++ L+++T+ + + ++ Q T N L+L FT E ++K+ + + +F +N FD V G + + L E+K + LR R++R+ K S+ L+ + + S +++ + LL+ L I+ ++GMQ+FG D+TQ+ + ++F NF LL +F + TGE W+ +M D G R G G +C+ YFI + +++LN+F+A+ +DN
Sbjct: 52 KCRAAVKSTAFYWMVIVLVFLNTLTIASEHYDQM--------QWLTHVQDTANKVLLAL----------FTLEMLVKIYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELKVMSPL----GISVLRCVRLLRIFKATRYWNSLSNLVASLLNSMRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQV-RRSSFDNFPTALLTVFQQILTGEDWNSVMYD---GIRAYGGPSFPGMLVCI--------------YFIILFICGNYILLNVFLAIAVDNL 293
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003634.1 (pep scaffold:Pmarinus_7.0:GL483626:1829:10505:1 gene:ENSPMAG00000003326.1 transcript:ENSPMAT00000003634.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 213.386 bits (542), Expect = 2.025e-63 Identity = 106/185 (57.30%), Postives = 138/185 (74.59%), Query Frame = 2
Query: 3686 KSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLD 4240
+S E ++ I ++ F RLFRVMRL+K+L +G IR LLWTFI+SF+ALP+V LLI ++FFIY +IGMQIFG I + D QIN+NNNF+ F +LLLFRCATGE+W I++ SG+RC + G ++ CGS+F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLD
Sbjct: 20 QSSEDSSRI-SITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVMMFFIYAVIGMQIFGKIAMQDGMQINRNNNFQTFPQAVLLLFRCATGEAWQEILLGCMSGRRCDPESDYNVG-----EEFTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLD 198
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Yeast
Match: CCH1 (Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function [Source:SGD;Acc:S000003449]) HSP 1 Score: 87.4261 bits (215), Expect = 1.642e-17 Identity = 57/190 (30.00%), Postives = 99/190 (52.11%), Query Frame = 2
Query: 3791 LLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMID-TESGKRCACGNLTTGGICLSPQDKLCGSD-FSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDP---SILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGF 4345
LL T + S + + G+LF +Y I QIFG L +T N NFR + +++LFRC+ GE W+ IM D T S C+ + +T CGS+ ++Y +++ +++ ++ +N+FV++I+ NF Y+ R S + + +Y+ W++FD GTG L + ++M P+ F
Sbjct: 1646 LLETAMASLPPILSLTYTWGVLFLVYAIALNQIFGLTRLGSNT--TDNINFRTVIKSMIVLFRCSFGEGWNYIMADLTVSEPYCSSDDNSTY--------TDCGSETYAYLLLMSWNIISMYIFVNMFVSLIIGNFSYVYRSGGSRSGINRSEIKKYIEAWSKFDTDGTGELELSYLPRIMHSFDGPLSF 1825 HSP 2 Score: 74.7146 bits (182), Expect = 1.364e-13 Identity = 72/271 (26.57%), Postives = 120/271 (44.28%), Query Frame = 2
Query: 260 FEYLVILTIIANC-VSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVT---IGLITVNLQADTVDLRTFRAIRVLRPFK-LVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ-SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKAT---------DSQWNVLYFVLLIVLGSFFMLNL 1027
F IL I+ +C V+ +H G + + ++ F+G F+ E I+K +A GFI NAYL++ WN +DF V+ I LI L+ + R F+ + LR + L + Q + + + + GL+ L + F + GL +++G TC D + CY + SN+ + P+ L D+ A +++Q I++EGW D+L ++ S N L+ VL L F+LNL
Sbjct: 1212 FALATILLIVCSCYVTPLYRMHHKMGTWNW----SSALDCAFIGAFSIEFIVKTVADGFIYSPNAYLRNPWNFIDFCVLISMWINLIAY-LKNNGNLSRIFKGLTALRALRCLTISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGRL-GTC--NDGSL----GRADCYNEYSNSVFQWDIMSPRVYQQPYLHL---DSFASAFSSLYQIISLEGWVDLLENMMNSSGIGTPATVMGSAGNALFLVLFNFLSMVFILNL 1467 HSP 3 Score: 68.1662 bits (165), Expect = 1.352e-11 Identity = 63/274 (22.99%), Postives = 122/274 (44.53%), Query Frame = 2
Query: 449 AYLKSVWNILDFIVVT---IGLITVNLQADT-VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTC-W--KTDSPFLFDATQQAC----------------YPQSNTSCNAGFTCKPYWD-----GPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFEN 1186
A+ +S WN +D + +G+ DT +R F+ + +LR +LV+ + +L + + L+ + ++++ + F I+G+++++G F C W D + Q C S S + GF C Y P+ G ++FDNI +M VF ++ +TD++Y T +D L+F++ I + + ++LNL++ VL F E +K++ R+T ++ GY + + +F N
Sbjct: 488 AFARSSWNRIDLVSSVSFWLGMFLSIKSYDTKTGIRIFKPLAILRILRLVNVDTGMPSILRGLKYGIPQLVNVSSMLVYFWIFFGILGVQIFQGSFRRQCVWFNPEDPTDTYQYDMQFCGGYLDPVTKRKQNYIYEDGSEGSVSKGFLCPQYSKCVSNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYT-MDSDEMAACLFFIVCIFVLTIWLLNLLIAVLVSSFEIANEEYKKKKFIYGSRKTGYVARIVTGYWKYFKLKANQTKFPN 760 HSP 4 Score: 64.6994 bits (156), Expect = 1.646e-10 Identity = 55/198 (27.78%), Postives = 93/198 (46.97%), Query Frame = 2
Query: 2534 DLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVI---IAVFSFFYSK-NLNTI-KSLRVIRVLRPLKTINRVPK---------LKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKN 3085
D AF F +E I+K + G + P +Y+R+PWN +D V+I I + ++ + NL+ I K L +R LR L N + L +FE + S+ F F V + +F G+ CND S + C + Q I + R++Q+ Y + D+ ++ +L+ + + EGW D+L+N
Sbjct: 1246 DCAFIGAFSIEFIVKTVADGFIYSPNAYLRNPWNFIDFCVLISMWINLIAYLKNNGNLSRIFKGLTALRALRCLTISNTARQTFNLVMFDGLNKIFEAGLISLS-----------LLFPFTVWGLSIFKGRLGTCNDGSLG-RADCYNEYSNSVFQWDIMS-PRVYQQPYLHLDSFASAFSSLYQIISLEGWVDLLEN 1430
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Nematostella
Match: EDO46991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNU9]) HSP 1 Score: 609.372 bits (1570), Expect = 0.000e+0 Identity = 357/824 (43.33%), Postives = 515/824 (62.50%), Query Frame = 2
Query: 2270 KKQPI--KESPVIVENPPAE--------------GIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY---SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQ---AKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIK--SHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDK--LCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMG-SVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNF 4660
+K PI ESP + E+ A ++ + + P SSLFIF N R VCH +A +F FI+++I SS LAAEDP+ NS RN++L +FD FT+VF +E+ +K+I G++LH GS+ R +N++D +VV +++ S S ++ ++ LRV+RVLRPL+ INR LK V + + +VK + NI++V +LF F+F VI VQLF G FF C D K K C+G + F G+ K R WQ FN++++ +M+TLFTV T EGWP +L++S+ +T +EGP N + +AI+Y+++ I+ FF +NIFV +IVTFQ +GE + D ELDKN+ KC++FA+ +PT RY+P N+ + + VW +V S PFEYLI I ++I L+++ + KL R L NI FT +F ECILK+ A++ K +F D WN+FDFV V+GS+ D+ ++E+ SE+ GF RLFR +RL+K+L +G I+ LLWTFI+SF+ALP+V LLI + FFIY ++GMQ+FG I ++ +TQIN+NNNF+ F L++LFR ATGE+W LIM+ C PQD LCG+DF+Y YF +F + +FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+VR+W+E+DP +G + D+ ++K++ PP+GFGK CP+R AC +L+ MNM L+ VNFN+TLF L+R SL IK ++ ++E+R ++KVWP Q +D + +TVGK YA LI + +R F
Sbjct: 766 RKHPITDAESPSLAEDSKATLNRDHESVRSATSTEVMDHEPMPPESSLFIFSNTNCFRVVCHRIATNSYFVNFILLLIIVSSCMLAAEDPLNSNSKRNQVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLFKGTFFSCTDAEKITKRECQGQYIEFKGP-GLTNPVVKDREWQPQTFNFNDVPQAMLTLFTVMTFEGWPGILESSMDSTDVDEGPFLNNRPWVAIYYVIYIIIIAFFMINIFVGFVIVTFQNEGEEEFKDCELDKNQRKCVEFALKARPTRRYIPTNR--LQFHVWRVVTSQPFEYLIFAFITGNTILLMMQYYNEPKLYTRV--------------------LDGFNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQMFA----FGFFRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMFGRIAIDPETQINRNNNFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKCDPNAY------PQDTDGLCGTDFAYAYFCSFYAICSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFVRVWSEYDPEASGCVKHVDIVTVLKRIAPPLGFGKFCPHREACKRLVSMNMPLNRDGTVNFNATLFGLVRTSLSIKRPEGKGSLEKANEEMRAIIMKVWPK-TSQEFLDEIIQPPGGDDEVTVGKFYATYLIQEYFRRF 1555 HSP 2 Score: 463.381 bits (1191), Expect = 7.367e-135 Identity = 304/664 (45.78%), Postives = 416/664 (62.65%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGL-------IEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQ---ADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWK--TDSPFLFDATQQACYPQSNTSCN---AGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEEL----IRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWI--------KYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNF-EQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
P R+LF + N IR + L + + F+ ++++TI ANC +LA LP+ D + I ++ E +FLGIFT E++LKIIA GF++ AYL++ WNILDF++V +GL T+ ++ D+ D++ RA RVLRP +LVSGVPSLQVVL SIIKA+ PL I LLV+F V+I+AIIG+E++ G H+TC+ T P FD SC+ G C W+GP +G+ NFDNIG A LTVFQCIT+EGWTDV+Y N A + W LYFV LI+ GSFF+LNLVLGVLSGEFAKE+ R +K F KLRE Q ++ GY++W+ AE++ E+++ ++K + R++ + + K Q++ + WI ++ H +R + +KTQ FYW VI V LN+ +A+EHY QP+FL +FL A +FL++F E+ +K++ G YF S FN FD +VV SL+E ++ G S LRC+RLLR+ K+TRYWSSL NLV SLL+SMRSI+ LLLLL LF+ I +LLGMQ+FGG FN ++ P NFD+F A++TVF ILTGE+WN VMY GI+S + S S I YF+ +V+ GNY LLNVFLAIAVD
Sbjct: 43 PKRALFCLTLGNPIRSTAISLFTVFFLDVFRTPFDVMILITIFANCAALAAFQPLPEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQP-TFDEPHPCSTESEGYSCSNAGPGQVCLKKWEGPNYGITNFDNIGLACLTVFQCITLEGWTDVMYSINDAIGNSWPWLYFVTLIIWGSFFVLNLVLGVLSGEFAKEKARAQKSGEFQKLREKQLVDDAYHGYLDWISQAEDIEGDSSAGEDEEGKADRKPSFRRRKENDDISKNKENQEDSAASD--------------QGWIDRKKKILKRFHHRLRRSCRKAVKTQWFYWTVIVFVFLNSLTLALEHYNQPEFLTQFLDKANKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISSLLELGLTEAMDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGGKFNLGDEDVPRSNFDSFWRALVTVFQILTGEDWNAVMYTGIQSWGGITES--LSAIPILYFIFLVVVGNYILLNVFLAIAVD 689 HSP 3 Score: 117.087 bits (292), Expect = 5.434e-26 Identity = 84/283 (29.68%), Postives = 143/283 (50.53%), Query Frame = 2
Query: 140 RQTLSISFVGQKP-NPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDL---RTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPY-----WDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD 961
R S + +P P+ SLFIFS N R C + +F ++L II + LA E L + V + + F +FT E +KIIA G I+ K ++ +S +N+LDF+VV + ++++ L+ + + R R +RVLRP + ++ L+ V+ + A+ + I L+ + +FA+IG+++++G F +C TD+ + T++ C Q G T P W F NF+++ AMLT+F +T EGW +L + +TD
Sbjct: 794 RSATSTEVMDHEPMPPESSLFIFSNTNCFRVVCHRIATNSYFVNFILLLIIVSSCMLAAEDPLNSNSKRNQV--LNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLFKGTFF-SC--TDAEKI---TKRECQGQYIEFKGPGLT-NPVVKDREWQPQTF---NFNDVPQAMLTLFTVMTFEGWPGILESSMDSTD 1064 HSP 4 Score: 114.005 bits (284), Expect = 3.813e-25 Identity = 81/234 (34.62%), Postives = 130/234 (55.56%), Query Frame = 2
Query: 2429 FDIFIMMVIFASSMALAAEDPVIF--NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF---FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFF---YCN-------DLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
FD+ I++ IFA+ ALAA P+ +SL N LE + F +F +E +LK+I G V+HPG+Y+R+ WNI+D ++V++ + + Y+ + +K+LR RVLRPL+ ++ VP L+ V +II ++ +F+I ++ + I+ +I V+LF GK Y N D +GY + N G K+ NY N+DNI + +T+F T EGW DV+
Sbjct: 76 FDVMILITIFANCAALAAFQPLPEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQPTFDEPHPCSTESEGYSCS-NAGPGQVCLKKWEGPNYGITNFDNIGLACLTVFQCITLEGWTDVM 308 HSP 5 Score: 81.6481 bits (200), Expect = 2.821e-15 Identity = 74/270 (27.41%), Postives = 131/270 (48.52%), Query Frame = 2
Query: 3383 FEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
F + +++ + L+S+TL ++ H PE T+FL + N F +FT E ++KM F +F +N FD + V+ SL ++ L+E + + LR R++R+ KV S+ L+ + + S +++ + LL+ L I+ ++GMQIFG + N + +NF +F L+ +F+ TGE W+ +M G + GGI S S YFI ++V +++LN+F+A+ +DN
Sbjct: 460 FYWTVIVFVFLNSLTLALE-HYNQPEFL----TQFLDKA-------------NKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISSLLELGLTEAMDQRPI----GISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGG-KFNLGDEDVPRSNFDSFWRALVTVFQILTGEDWNAVMY---------TGIQSWGGITESL------SAIPILYFIFLVVVGNYILLNVFLAIAVDNL 691 HSP 6 Score: 69.3218 bits (168), Expect = 1.695e-11 Identity = 73/251 (29.08%), Postives = 128/251 (51.00%), Query Frame = 2
Query: 1382 IKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG---DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMY------------NGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
+ +QPF + + + NT + +++Y +PK R L F S+F+ E +K+F F ++YF ++N+FDF+VV S+V+ ++ +Q F F R R LRL+KL S ++ L+++ + S +++ + LL+ + FI ++GMQ+FG D + + NF TFP +++ +F TGENW +M N DF+ Y F I F ++N+F+A+ +D
Sbjct: 1151 VTSQPFEYLIFAFITGNTILLMMQYYNEPKLYTRVLDGFNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQMFAFGFFRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMFGRIAIDPETQINRNN-NFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKCDPNAYPQDTDGLCGTDFAYAYFCSFYAICSF---LIINLFVAVIMD 1397 HSP 7 Score: 68.5514 bits (166), Expect = 2.881e-11 Identity = 73/318 (22.96%), Postives = 131/318 (41.19%), Query Frame = 2
Query: 3368 VVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF--------------------------------------GNIELNDDTQINQN-NNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
V PF+ +MI+I + + + + LPE+ ++ + E+ L+ F IFT E +LK+IAY F + + ++ WNI DFV V+ L + + + T + ++ LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G + L N NF N + L +F+C T E W +M G+ + + YF+T I+ +F +LNL + V+ F
Sbjct: 71 VFRTPFD--VMILITIFANCAALAAFQPLPEQ----DSSLINEE---------LEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIV-----KLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQPTFDEPHPCSTESEGYSCSNAGPGQVCLKKWEGPNYGITNFDNIGLACLTVFQCITLEGWTDVMYSINDA---------------------IGNSWPWLYFVTLIIWGSFFVLNLVLGVLSGEF 347 HSP 8 Score: 50.8322 bits (120), Expect = 5.918e-6 Identity = 42/164 (25.61%), Postives = 80/164 (48.78%), Query Frame = 2
Query: 242 LIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD----LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGI 721
++ + FEYL+ I N + L ++ + + + G F +F E ILK+ A KN +L WN+ DF+VV ++ + + + + FR R LR KL++ ++ +L + IK+ L +GLL++ I+A++G++++ I
Sbjct: 1150 VVTSQPFEYLIFAFITGNTILLMMQYY---NEPKLYTRVLDGFNIGFTSVFLLECILKLFAFK---PKNYFLDR-WNLFDFVVVVGSVVDITMNEVSSEQMFAFGFFRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMFGRI 1306
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Nematostella
Match: EDO35497 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLD1]) HSP 1 Score: 192.2 bits (487), Expect = 7.419e-52 Identity = 161/556 (28.96%), Postives = 289/556 (51.98%), Query Frame = 2
Query: 2411 VANLRFFDIFIMMVIFASSMALAAEDPVIF-NSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVV-------IIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPAN---KNSVYY--------KVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDD---TQINQNNNFRNFMMGLLLLFRCATGESWHLIM 4012
V + R FD I++ I S LA E P I L+ ++++ L FT +F +EM++K++ +GLVL PG+Y+RD W+++D +V I+ S + L T++ R +R LRPL+ I R P LK V +T++ S+K + N +++ +F +FG++ VQLF GKF+Y C+G + + Q + + W +N+D+++ ++I+LF V+T +GW +++ + I A + PI NY + +++I F ++ F +N+ V +++ FQ E +D+ E +P + A ++S+YY K+ + ++ I II ++ I + ++ H K+P+ ++ F FT +F E ++K+IA F + KD WN+ D VL S+T + L + + + LR+ R+ R++K++ +R LL T ++ +P + LL LLFFIY +G+Q+FG++E + D N++ +FR+F +L LFR ATG++W+ I+
Sbjct: 15 VCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLKRQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYY-----------CEGDSHVISKQECANSTRGQWVNRRYNFDDLLQALISLFVVSTKDGWVEIMHHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVVVENFQRCRE------RMDEEE--------QARPHRKGKKARNQMQDSLYYESYGPLRLKIHFICTHRNWDITIAAIICINVICMSLE-HYKMPQVFVETTNYF--------------------FTSVFVIEVVVKVIALGFVRYPKDRWNLIDLAIVLLSVTGIVLELLVKVDHLFNPTVIRTLRVLRITRVLKLVKLAKGVRSLLDTLFEALPQVPNLGLLFFLLFFIYSCLGIQLFGSLECSHDYPCQGFNRHAHFRDFGTAMLTLFRIATGDNWNGIL 524 HSP 2 Score: 122.479 bits (306), Expect = 1.031e-28 Identity = 145/613 (23.65%), Postives = 284/613 (46.33%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALEVH--LPQG-DRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLI------TVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD---------SQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVI--TLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQII-------NPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGG-----DFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNG 1963
R+ + + R F+Y++++ II +C LA+E QG R I M +F IFT E ++K++A+G ++G YL+ W++LD +V + I T ++ + + LR FRA+R LRP +++ P L++V+ +++ ++ P+ L+ ++F I+G+++++G F+ + DS + ++Q C S + + Y NFD++ A++++F T +GW ++++ A D ++W ++YF+ ++LG F +LN+++GV+ E R + + EE+ + +K + ++ + + Y +R + HFI T W + ++ +N C+++EHY P+ F+ Y F S+F+ E+ +K+ G Y + ++N+ D +V +S+ + +++ NP LR +R+ R+LKL + +R+L+ +L ++ + +L LL L FI + LG+QLFG D+ + +F F A++T+F I TG+NWN ++ G
Sbjct: 9 RQRMRAVCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLKRQIIDISML----VFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYYC--EGDSHVI---SKQEC----ANSTRGQWVNRRY---------NFDDLLQALISLFVVSTKDGWVEIMHHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVV---------------------------------------VENFQRCRERMDEEEQARPHRKGKKARNQMQDSLYYESY-------------------------GPLRLKI-HFICTHR-NWDITIAAIICINVICMSLEHYKMPQV---FVETTNYFFTSVFVIEVVVKVIALGFVRYPKDRWNLIDLAIVLLSVTGIVLELLVKVDHLFNPTV---IRTLRVLRITRVLKLVKLAKGVRSLLDTLFEALPQVPNLGLLFFLLFFIYSCLGIQLFGSLECSHDYPCQGFNRHAHFRDFGTAMLTLFRIATGDNWNGILKVG 527 HSP 3 Score: 69.3218 bits (168), Expect = 9.773e-12 Identity = 70/291 (24.05%), Postives = 135/291 (46.39%), Query Frame = 2
Query: 3383 FEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF--------------GNIELNDDTQ---INQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
F+Y+I++ I S L I++ P+ A Q + + + S + FTIIFT E ++K++A L + +D W++ D V+ S D+ ++ S S + L R R +R ++V+ R +++++ T + S K + L+ + F ++ I+G+Q+F E + T+ +N+ NF + + L+ LF +T + W IM + + + Q + +++ YFI F+L+ FL+LN+ V V+++NF
Sbjct: 21 FDYVILVFIIFSCAVLAIEA----PDIAEQGLKRQIIDISM------------LVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVT-YTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYYCEGDSHVISKQECANSTRGQWVNRRYNFDDLLQALISLFVVSTKDGWVEIM------------HHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVVVENF 282 HSP 4 Score: 53.9138 bits (128), Expect = 5.293e-7 Identity = 64/248 (25.81%), Postives = 122/248 (49.19%), Query Frame = 2
Query: 2372 FGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED---PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSK--------NLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLK 3082
+GP +R HF+ R +DI I +I + + ++ E P +F +E + FTSVF++E+++KVI +G V +P +D WN++D+ +V+++V N I++LRV+R+ R LK + ++++ +T+ ++ V N+ ++F L FI+ + +QLF G +D C+G+ + ++ + +M+TLF + TG+ W +LK
Sbjct: 317 YGP---LRLKIHFICTHRNWDITIAAIICINVICMSLEHYKMPQVF-------VETTNYFFTSVFVIEVVVKVIALGFVRYP----KDRWNLIDLAIVLLSVTGIVLELLVKVDHLFNPTVIRTLRVLRITRVLKLVKLAKGVRSLLDTLFEALPQVPNLGLLFFLLFFIYSCLGIQLF-GSLECSHDYP------CQGF------------------NRHAHFRDFGTAMLTLFRIATGDNWNGILK 525
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Nematostella
Match: EDO46797 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RP56]) HSP 1 Score: 192.971 bits (489), Expect = 2.542e-49 Identity = 194/664 (29.22%), Postives = 320/664 (48.19%), Query Frame = 2
Query: 2396 RVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLR-NRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVV--IIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYC-NDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTF----QEQGESDKSDLELDKNETKCIDF----AINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCAC----GNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSI-LGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPP 4336
RV FV + R+F+ FI+ + SS L ED + R L F + F ++F LE ILKVI G V SY WN +D ++V ++V F + NL+ +S R +R LRPL+ I+R+ +K V + +++ + ++L+V +LF +F + VQLF+GKF+ C N +++ + N + W N+DN++N + L V T EGW +V+ +++ +T ++ P +++VF IV FF +N+FV +II F ++ E + + L +++ K + A P++R P + K++ LV S FE I+ +I + ++++ + + P L+ N IF E I+K+IA + +FK W+IFDFV V+ S+ ++L M +E + L LR+FRV RL++V+ IR LL I S AL + L+ L+ FIY IIGM FGNI+ +N NF F ++L+FR TG W+ I+ D + C G L G C P G+ F Y +T+I+ +++N+++AV++DN + + L Y +A F MG +L + + + L P
Sbjct: 785 RVRCFVEH-RYFEWFILFTVMVSSFTLVFEDIHLHQKPDLERALNFLNFLFAAIFSLEFILKVIGFGFV----SYFTSVWNCLDALIVSVCVSVACLFENPNLSVFRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGKFYKCVNAEDEKLSPSVIP-----NKTACLDHPDYRWVNANVNFDNVINGFLALLQVATYEGWMEVMADAVDSTKIDQQPSYEANLIAYAYFVVFIIVGSFFVLNLFVGVIIDNFNTLKKKYEEINSMGMLLTESQRKWVQILMAAAKRKPPSNRLRPTD--CFRGKLFDLVSSNQFEVFILTVIMCNMGMMMVQHYDQPP---------------------LTTVIGNYVLVGIFLLEAIIKIIAMRLH-YFKKAWSIFDFVVVVSSIVGMYLG-MSTESAVVSPGLLRVLRVFRVARLLRVVQFAKRIRQLLVAIIISMPALFNIGTLLFLVMFIYAIIGMSSFGNIK--KQGALNDLVNFETFSSSIILIFRLGTGTGWNDIL-DALVVQPPHCDPNFGGLPNGN-CGFP----IGAVF---YLVTYIVSVFLIIVNMYIAVLLDNVNRAHENDEFGLSKDKFKSYYHTFANFS-MGKQFLPLRVLPEFLAALDKP 1401 HSP 2 Score: 137.887 bits (346), Expect = 1.895e-32 Identity = 92/296 (31.08%), Postives = 150/296 (50.68%), Query Frame = 2
Query: 191 SLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCW----------KTDSPFLFDATQQAC-YPQSNTSCNAGFTCKPY-WDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
SL + +R C LI +FE +V L I+ NC+ L L + E IF I+T E I KII+ GF+ AYL+ WN LD +VV + ++ L D L R +RVLR F+ +S + L+ ++ S+++++ L + +L LF++ + A+IGL+++ G N C S + + C + + C A +TC + P G NFD+IG++++ FQ +TM+ W + LY + +W ++YFV+ I SF++LNLVL V+
Sbjct: 40 SLLCLGPVHPLRMFCILLITHPYFEIIVTLVILVNCIILGLNDPPEEA------------EYIFTAIYTLEMICKIISRGFVKDSYAYLRDTWNWLDSLVVFLSYLS--LAPDIASLSGIRTLRVLRAFRTISALKGLRAMVNSLLRSLKLLTDVLVLFLFSLCVLALIGLQLFTGELRNKCVLETANATTINNPSSWYHREGEPLLCGFSSTAGRCPANYTCVAHVGPNPDHGFTNFDDIGWSLVMAFQILTMDFWEN-LYDRVLRSMGEWYMMYFVVGIFSCSFYLLNLVLAVV 320 HSP 3 Score: 129.798 bits (325), Expect = 5.040e-30 Identity = 148/600 (24.67%), Postives = 275/600 (45.83%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALE-VHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNL--QADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFF----GLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKAT--DSQ----WNVL---YFVLLIVLGSFFMLNLVLG-VLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIF------QIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVM 1954
R + +E R+FE+ ++ T++ + +L E +HL Q + + + + +F IF+ E ILK+I GF+ +Y SVWN LD ++V++ + L + R+FR +R LRP + +S + ++VV+ ++ A+ + + ++ + L+F+I G++++ G F+ D + + P + T+C D P + VNFDN+ L + Q T EGW +V+ +T D Q N++ YFV+ I++GSFF+LNL +G ++ +++ E + L E+QR W++ A K++ NR + + K+ + + F ++T+++ N + V+HY QP YV + IF+ E IKI + YF+ +++FDF+VV S+V +++P LR R+ RLL++ ++ +R L+ +++ SM ++ ++ LL L +FI ++GM FG +NF+TF ++II +F + TG WN+++
Sbjct: 783 RCRVRCFVEHRYFEWFILFTVMVSSFTLVFEDIHLHQ--KPDLERALNFLNFLFAAIFSLEFILKVIGFGFV----SYFTSVWNCLDALIVSVCVSVACLFENPNLSVFRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGKFYKCVNAED-----EKLSPSVIP-NKTAC---------LDHPDYRWVNANVNFDNVINGFLALLQVATYEGWMEVMADAVDSTKIDQQPSYEANLIAYAYFVVFIIVGSFFVLNLFVGVIIDNFNTLKKKYEEINSMGMLLTESQR---------KWVQILM----------AAAKRKPPSNRLRPTDCFRGKLFD-------------------------------------LVSSNQFEVFILTVIMCNMGMMMVQHYDQPPLTT---VIGNYVLVGIFLLEAIIKIIAMRLH-YFKKAWSIFDFVVVVSSIVGMYLGMSTESAVVSPGL---LRVLRVFRVARLLRVVQFAKRIRQLLVAIIISMPALFNIGTLLFLVMFIYAIIGMSSFGNIKKQGALNDLVNFETFSSSIILIFRLGTGTGWNDIL 1298 HSP 4 Score: 121.709 bits (304), Expect = 1.797e-27 Identity = 153/656 (23.32%), Postives = 292/656 (44.51%), Query Frame = 2
Query: 221 IRRSCKGLIE--QR-WFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVE--HYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSL-VEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDF----NFEQGK----------------------PTLNFDTFPNAIITVFVILTGENWNEVMYNGIRS-QMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
+ R+C L+E QR F+ ++ I+ N V L + H D TF++ + IF G+F E I+KI A G + Y+ + WN+ D +++ + L T + FR +R+LR L ++ ++ +I ++ P+ I ++ + +FA++G+ V+ + T+ G P W NF + ++ + VF+ + E W + L+ + T++ +L+F+ +++G+F +LNL + +L FA K E E Y+N L R ++ + + N + TK ++ C + W+K+ R V+ F++ + F W ++ V++++ + E H Q L+R L + ++F +IF E +K+ GFG YF S +N D ++V+V + V +F+ NP S F + R +R LR L+ ++ ++ +L +++ I S+L++ LF + ++ G+QLF G F N E K +NFD N + + + T E W EVM + + S ++ S + ++I +YF++ +I G++ +LN+F+ + +D
Sbjct: 466 LSRACSALVEFIQRPLFDGIIAGLILLNTVVLTMYYHGMPDDLTFVLDIL---NAIFTGLFILEMIVKIFAFGLV----KYICNKWNLFDSVIIITSAVAYFLGTST-GVSIFRILRLLRVLYLAQSWETMAKLIRAIAHSVDPIFNITCILGVIIYVFAVVGMRVFGPHY-------------------------TAEAFGSRGIPRW--------NFTDFTHSFMMVFRILCSE-WVEPLWDCMRVTNTA-ALLFFLPALIVGNFIVLNLFVALLLNSFA-------------KKEEDLHREASEQPYVNPCSCLAHLTR--TRKTRMYYTQAVNNNKNTKRYIVEIDDCFPECCMNACGCTSGQQHFC----WLKF----RCRVRCFVEHRYFEWFILFTVMVSSFTLVFEDIHLHQKPDLERALNFLNFLFAAIFSLEFILKVIGFGFVSYFTSVWNCLDALIVSVCVSVACLFE--NPNLSV-FRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGKFYKCVNAEDEKLSPSVIPNKTACLDHPDYRWVNANVNFDNVINGFLALLQVATYEGWMEVMADAVDSTKIDQQPSYEANLIAYAYFVVFIIVGSFFVLNLFVGVIID 1052 HSP 5 Score: 80.4925 bits (197), Expect = 5.945e-15 Identity = 51/177 (28.81%), Postives = 99/177 (55.93%), Query Frame = 2
Query: 2357 SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF 2887
+SL GP + +R C + +F+I + +VI + + L DP E + FT+++ LEMI K+I G V +Y+RD WN +D +VV ++ S + ++ ++ +R +RVLR +TI+ + L+A+ +++ S+K + ++L++F+ + +I +QLF+G+
Sbjct: 39 ASLLCLGPVHPLRMFCILLITHPYFEIIVTLVILVNCIILGLNDPP----------EEAEYIFTAIYTLEMICKIISRGFVKDSYAYLRDTWNWLDSLVVFLSYLSL--APDIASLSGIRTLRVLRAFRTISALKGLRAMVNSLLRSLKLLTDVLVLFLFSLCVLALIGLQLFTGEL 203 HSP 6 Score: 69.707 bits (169), Expect = 1.270e-11 Identity = 64/263 (24.33%), Postives = 105/263 (39.92%), Query Frame = 2
Query: 170 QKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVL 937
+KP +R + R L+ FE ++ I+ N + + + H Q T ++ + +GIF EAI+KIIA+ + Y K W+I DF+VV ++ + L T LR R RV R ++V ++ +L +II +M L IG L+ + I+AIIG+ + I G LVNF+ +++ +F+ T GW D+L
Sbjct: 1088 RKPPSNR----LRPTDCFRGKLFDLVSSNQFEVFILTVIMCN-MGMMMVQHYDQPPLTTVIGNY-----VLVGIFLLEAIIKIIAM-----RLHYFKKAWSIFDFVVVVSSIVGMYLGMSTESAVVSPGLLRVLRVFRVARLLRVVQFAKRIRQLLVAIIISMPALFNIGTLLFLVMFIYAIIGMSSFGNIKK-------------------------------------QGALNDLVNFETFSSSIILIFRLGTGTGWNDIL 1298 HSP 7 Score: 60.4622 bits (145), Expect = 7.016e-9 Identity = 56/234 (23.93%), Postives = 105/234 (44.87%), Query Frame = 2
Query: 3509 NDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYS---IRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFG---NIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+D L LN FT +F E I+K+ A+ + + WN+FD V ++ S FL + +FR++RL++VL S + L+ S + + ++G++ +++ ++GM++FG E I + NF +F +++FR E W + D C +T L +F+ ++V F++LNLFVA+++++F
Sbjct: 506 DDLTFVLDILNAIFTGLFILEMIVKIFAFGLVKYICNKWNLFDSVIIITSAVAYFLGTSTG------------VSIFRILRLLRVLYLAQSWETMAKLIRAIAHSVDPIFNITCILGVIIYVFAVVGMRVFGPHYTAEAFGSRGIPR-WNFTDFTHSFMMVFRILCSE-WVEPLWD--------CMRVTNTAALL--------------FFLPALIVGNFIVLNLFVALLLNSF 703
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Nematostella
Match: EDO35661 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKR1]) HSP 1 Score: 189.119 bits (479), Expect = 4.611e-48 Identity = 187/705 (26.52%), Postives = 348/705 (49.36%), Query Frame = 2
Query: 2423 RFFDIFIMMVIFASSMALAAEDPVIFNS--LRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVF----SFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELD-------KNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSE--------ESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLI---MIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYP-YFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSI-LGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRE 4459
+ F+ I+ +I ASS++LA ED V +S ++L+ ++ F +F +EM+LK I +G +Y +PWNI+D ++VI+++ + F + + I+SLR +R RPL+ I+R +K V +++ ++ + N+L+V ++F IF ++ VQ+F GKF C D E C N + + R W+ + N+D + + L V T EGW +++++++ AT +E PIE +F++VF I+ FF +N+F+ +II F + + ++ +D +N + A KP R + +N +++ +V S FE IM+ I ++ + ++++ + L S + LN+ FT +F E IL++IA + K +F +PWN+FDFV V+ S+ + + +++ LR F R IR LL++ + S AL + L+ L+ FIY IIGM IFG+++ + +N NF F +LLFR T W+ I ++ +E L +G CG+ + P Y +F+++ +++N+++AVI++N++ + I + ++ + ++W FDP T ++ + D+ + ++ + K P + AC L N+ L +++ L AL++
Sbjct: 827 KVFEGIILFLIAASSISLAFED-VYLDSKPTLKQVLQILNILFAVIFTVEMLLKWIGLGF----KTYFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKFGKCVDEGG---EKCPASVVP-NKTVCLSNGYR-WENSNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKIDEQPIEENNVSAYLFFVVFIILGTFFTLNLFIGVIIDNFNQLKQQMEAVGSMDVFLTSTQRNWMNALKSAATKKPKKR-IRRPQNKFQGELYDMVQSRKFEVFIMLFITINMVVMMVQHYDHLA--------------------VWSKQILNLIFTSVFILEAILRIIALR-KGYFLNPWNVFDFVIVISSIIGIIVENLQTSLVINPSLLRVVRVFRVGRLLRFFEAAR---------GIRRLLFSLVISTPALFNIGALLFLIIFIYAIIGMSIFGHVKKTE--ALNDVVNFETFGSSFVLLFRLMTSAGWNDILDPLMISEPDCDPNYRGLPSGN---------CGNKYMAPIYLGSFVVIIFLILINMYIAVILENYNQVMEQEKIGITNEDIELFYQMWELFDPNATQYIPYADLSDFVHQMEGNLRIPK--PNKAACALL---NIPLVKGDKIHCLDLLQALVKR 1474 HSP 2 Score: 150.214 bits (378), Expect = 3.061e-36 Identity = 103/314 (32.80%), Postives = 164/314 (52.23%), Query Frame = 2
Query: 179 NPDRSLFIFSENNIIRRSCKGLIEQRW------FEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTC--------------WKTDSP--FLFDATQQACYPQSNT-SCNAGFTCKPYWDG-PFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSG 1048
N +SL++F N IR+ LI ++ V+LTI+ NCV LA+ + P+ E +F I+T E KIIA GF + + AYL+ WN LDFIVV +G +T++ D +L RV R + +S V L+ ++ +++ +M L + +L LF + IFA+IG++++ G N C + ++S +L D C + + +C +TC P+ G P FG +FD+ G+A+LT FQ IT++ W +V Y T W V+YF ++I G FF++NLVL V++
Sbjct: 19 NRSKSLYLFGPENPIRQFSLKLITNQYPLQLHNRHMFVLLTILVNCVFLAM-TNPPEQP-----------EYVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVTIS--PDVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGELRNKCALPVPENLSVPYRTYASNSSIWYLEDNEPILCGNTTGSRTCPTNYTCLPHAGGNPNFGYTSFDHFGWALLTGFQLITLDFWENV-YNNVIYTMGPWYVVYFAVVIFFGPFFLVNLVLAVVAA 317 HSP 3 Score: 123.635 bits (309), Expect = 4.827e-28 Identity = 162/658 (24.62%), Postives = 293/658 (44.53%), Query Frame = 2
Query: 218 IIRRSCKGLIEQRWFEYLVILTIIANCVSLALE-VHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVV----TIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD------SQWNV---LYFVLLIVLGSFFMLNLVLGV-LSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQ------IINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVM-------------YNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
++R + L+E + FE +++ I A+ +SLA E V+L V ++ I +F IFT E +LK I LGF Y + WNILDF++V + L T+ +R+ R +R RP + +S ++VV+TS++ A+ + + L+ L LIF+I+G++++ G F C + + P + G+ W+ +NFD + L + Q T EGW +++ AT + NV L+FV+ I+LG+FF LNL +GV + +++ + L TQR NWM ++A KK + R+ + K + + Y ++++ F ++ + +N + V+HY + + +F S+F+ E ++I + YF + +NVFDF++V S++ I + +INP R RLLR + +R L+FSL+ S ++ ++ LL L IFI ++GM +FG E +NF+TF ++ + +F ++T WN+++ Y G+ S ++ + IY F++I+ L+N+++A+ ++
Sbjct: 815 VMRYKVRRLVEHKVFEGIILFLIAASSISLAFEDVYLDSKPTLKQVLQILNI--LFAVIFTVEMLLKWIGLGF----KTYFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKF-GKCVDEGG----EKCPASVVPNKTVCLSNGYR----WEN---SNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKIDEQPIEENNVSAYLFFVVFIILGTFFTLNLFIGVIIDNFNQLKQQMEAVGSMDVFLTSTQR---------NWMNAL---------KSAATKKPKKRIRRP-----QNKFQGELY---------------------------------DMVQSRKFEVFIMLFITINMVVMMVQHYDHLAVWSKQIL--NLIFTSVFILEAILRIIALR-KGYFLNPWNVFDFVIVISSIIGIIVENLQTSLVINPSLLRVVRVFRVGRLLRFFEAA---RGIRRLLFSLVISTPALFNIGALLFLIIFIYAIIGMSIFGHVKKTEALNDVVNFETFGSSFVLLFRLMTSAGWNDILDPLMISEPDCDPNYRGLPS--GNCGNKYMAPIYLGSFVVIIFL---ILINMYIAVILE 1387 HSP 4 Score: 106.301 bits (264), Expect = 7.551e-23 Identity = 92/315 (29.21%), Postives = 144/315 (45.71%), Query Frame = 2
Query: 3350 YKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFG----------------NIELNDDTQI-------NQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILG 4225
YKV LV FE +I+ +IA SSI+L E + + L++ L+ LNI F +IFT E +LK I FK +F +PWNI DFV V+ S+ V L+ + + I +L LR FR +R I R +++++ + + + + V L+ + + I+ I+G+QIFG ++ N + N N NF G L L + AT E W IM D + + + +Y +F+ FI++ TF LNLF+ VI+DNF+ L + +G
Sbjct: 818 YKVRRLVEHKVFEGIILFLIAASSISLAF-------EDVYLDSKPTLKQ---------VLQILNILFAVIFTVEMLLKWIGLGFKTYFTNPWNILDFVIVIVSIM-VSLATIFGNDQIAFIRSLRTLRAFRPLRAIS---RFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKFGKCVDEGGEKCPASVVPNKTVCLSNGYRWENSNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKIDEQPIEENNVS------------AYLFFVVFIILGTFFTLNLFIGVIIDNFNQLKQQMEAVG 1100 HSP 5 Score: 75.0998 bits (183), Expect = 3.058e-13 Identity = 48/153 (31.37%), Postives = 83/153 (54.25%), Query Frame = 2
Query: 3539 NIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNN-NFRNFMMGLLLLFRCATGE 3994
N FT IF E ILK+IA F + + WNIFD + V+ S+ D +++ + T I LR FR++R++K+ ++ LL T +S AL + +++ ++ +I+ ++GMQ+ GN D + NF++F +++FR GE
Sbjct: 539 NEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGI---SVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSYTPDKFGGSIPRWNFKDFPHSFMMIFRVLCGE 688 HSP 6 Score: 72.7886 bits (177), Expect = 1.189e-12 Identity = 55/182 (30.22%), Postives = 98/182 (53.85%), Query Frame = 2
Query: 2360 SLFIFGPHNFIRRVC------HFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKF 2887
SL++FGP N IR+ + L +F+++ I + + LA +P E + F +++ +EM K+I G LH +Y+RD WN +D IVVI+ + S ++ + + RV R L+TI+ V LKA+ T++ S+K +++++++ + F IF +I +QLF G+
Sbjct: 23 SLYLFGPENPIRQFSLKLITNQYPLQLHNRHMFVLLTILVNCVFLAMTNPP----------EQPEYVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVTI--SPDVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGEL 192 HSP 7 Score: 67.0106 bits (162), Expect = 7.396e-11 Identity = 71/226 (31.42%), Postives = 123/226 (54.42%), Query Frame = 2
Query: 1409 VITLVLLNTACVAVEHYGQPKFLDRFLFY---AEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQ-SFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFN---FEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLN 2065
+I +LLNT +++EH P+ D L + VF IF+ E+ +K+ G Y R +N+FD IVV +S+V+ I P G S LR RLLR+LKL + WS++ +L+ ++ S+ ++ +L ++L + ++I ++GMQL G + F P NF FP++ + +F +L GE W E +++ + + + +M+ F+ I GN+ +LN
Sbjct: 509 IIVCILLNTLVMSIEH---PRLEDPLLTVVNISNEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSYTPDKFGGSIPRWNFKDFPHSFMMIFRVLCGE-WIEPLWDCMLA------TGPVAML---LFVPAFILGNFIILN 721 HSP 8 Score: 65.0846 bits (157), Expect = 3.100e-10 Identity = 67/259 (25.87%), Postives = 116/259 (44.79%), Query Frame = 2
Query: 263 EYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLI--TVNL---QADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLN 1024
E +I+ I+ N + +++E H D V ++ +F IF E ILK+IALGF+ Y++ WNI D IVV I ++ VN A + R R+LR KL ++ +L +I K++ L + +++ V IFA++G+++ + +P F + P W NF + ++ + +F+ + E W + L+ AT +L FV +LG+F +LN
Sbjct: 506 EIFIIVCILLNTLVMSIE-HPRLEDPLLTVVNIS--NEVFTFIFLLEMILKLIALGFL----GYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSY--------TPDKFGGS------------------IPRW--------NFKDFPHSFMMIFRVLCGE-WIEPLWDCMLATGPV-AMLLFVPAFILGNFIILN 721 HSP 9 Score: 61.6178 bits (148), Expect = 3.338e-9 Identity = 46/137 (33.58%), Postives = 73/137 (53.28%), Query Frame = 2
Query: 2414 ANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLA---FTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNL-----NTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSV 2800
N + +IFI++ I +++ ++ E P L + LL +++ FT +FLLEMILK+I +G + Y+R WNI D IVVII++ F +K + I LR R+LR LK + ++ TI S+
Sbjct: 500 TNGKVMEIFIIVCILLNTLVMSIEHP----RLEDPLLTVVNISNEVFTFIFLLEMILKLIALGFL----GYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSL 628 HSP 10 Score: 53.1434 bits (126), Expect = 1.392e-6 Identity = 61/222 (27.48%), Postives = 97/222 (43.69%), Query Frame = 2
Query: 3548 FTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTV-LGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFF-IYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGES--WHLIMIDTESGKRCACGNLTTGGICLSPQDKLC------GSDFSYPYFITF--ILVTTFLMLNL 4165
F I+T E K+IA F L + +D WN DF+ V LG++T + ++ NL + FRV R ++ + ++ ++ T + S K L W +++ L F I+ +IGMQ+F EL + + N L + +R S W+L E + CGN T C P + C +F Y F F L+T F ++ L
Sbjct: 80 FAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVT-----------ISPDVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKML-WDVMVLTLFFICIFALIGMQLFIG-ELRNKCALPVPEN-------LSVPYRTYASNSSIWYL-----EDNEPILCGNTTGSRTC--PTNYTCLPHAGGNPNFGYTSFDHFGWALLTGFQLITL 274
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Nematostella
Match: EDO36844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHI0]) HSP 1 Score: 159.073 bits (401), Expect = 7.578e-39 Identity = 102/305 (33.44%), Postives = 166/305 (54.43%), Query Frame = 2
Query: 185 DRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVH--LPQG-DRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKA---------TDSQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
D SL++F+ +N R K L + +WF+ V+L I+ NCV +ALE LP +R I M +FLGIFT E ++K+IALG +G +AYL+S WN++D +V I + V + A T LR FRA+R LRP +++S P L++V+ ++I ++ P+ I L+ +IF I+G+++++G FH C P T+ C + N + NFDN+ A+LT+F T +GW +++Y A +++NV+YFV ++L F +LN+++GV+
Sbjct: 908 DWSLYVFAPDNRFRMLNKELYQNKWFDRTVLLFILLNCVVMALEGPSVLPGSLERRVIDICMY----VFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGKFH-YCTDATVPV---TTKTECLENGGSWVNREY--------------NFDNLAKALLTLFVFSTKDGWVNIMYDGIDAVGIDKQPIRNHARYNVIYFVGFLLLAGFVVLNMLVGVV 1190 HSP 2 Score: 133.65 bits (335), Expect = 4.350e-31 Identity = 87/269 (32.34%), Postives = 146/269 (54.28%), Query Frame = 2
Query: 2360 SLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDP-VIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSL-------RVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRI--KETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQ 3136
SL++F P N R + + ++FD +++ I + + +A E P V+ SL R+++ F +F +EM++KVI +GL + P +Y+R WN+MD +V+I+ + N S+ R +R LRPL+ I+R P LK V ET+ISS+K + NI+++ F IFG++ VQLF GKF YC D + + K C W +N+DN+ +++TLF +T +GW +++ + I A ++ PI N+ +
Sbjct: 910 SLYVFAPDNRFRMLNKELYQNKWFDRTVLLFILLNCVVMALEGPSVLPGSLERRVIDICMYVFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGKFHYCTDATVPVTTKTEC-------------LENGGSWVNREYNFDNLAKALLTLFVFSTKDGWVNIMYDGIDAVGIDKQPIRNHAR 1165 HSP 3 Score: 103.605 bits (257), Expect = 6.837e-22 Identity = 77/274 (28.10%), Postives = 137/274 (50.00%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQ-IGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
R C ++ +WF Y+++ I N +++ +E + G + + IF IF E I+K+I LGF Y+K +NI D +V I ++ + D + R+ R+LR FKLV +P+L+ L +I M ++ + LLV+F + +I+G+ ++ G + + D + T +A NFD++ +A++TVFQ +T E W V+Y +AT S+W LYF+LL+ +G++ + NL++ +L
Sbjct: 401 RDGCASSVDSKWFMYVIMGAIFVNTLTMGIEYY---GQPQKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFY----GYIKDAFNIFDGTIVIISVVEL-FGDDDSGISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIF-MFTASILGMNLFGGKYR---FPNDEGVM--ETSRA---------------------------NFDDLFWAIVTVFQVLTQEDWNIVMYDGMRAT-SKWAGLYFILLMTIGNYILFNLLVAIL 632 HSP 4 Score: 101.293 bits (251), Expect = 3.041e-21 Identity = 83/236 (35.17%), Postives = 134/236 (56.78%), Query Frame = 2
Query: 1397 FYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTL-----NFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
F + ++ + +NT + +E+YGQP+ + L Y+F +IF E+ +K+ G G Y + FN+FD +V +S+VE G S LR RLLR+ KL R+ +LR + ++ +M ++++ L LL +F+F ++LGM LFGG + F + + NFD AI+TVF +LT E+WN VMY+G+R+ K + YF+L++ GNY L N+ +AI V+
Sbjct: 413 FMYVIMGAIFVNTLTMGIEYYGQPQKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGD-----DDSGISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKYRFPNDEGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMRATSKWAG---------LYFILLMTIGNYILFNLLVAILVE 634 HSP 5 Score: 85.1149 bits (209), Expect = 2.463e-16 Identity = 62/222 (27.93%), Postives = 102/222 (45.95%), Query Frame = 2
Query: 539 LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGI----------------------FHNTCWKTD---------------SPFLFDATQQA-----CYPQSNTSCNAGFTCKPYWD-----------GPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLS 1045
+ RAIRVLRP + ++ VPS+++++T ++ + L + + IF I+G+++++G+ F+ +D S F+ +T Y Q + N C W+ P + V+FDNI A + +FQ IT+EGWTD++Y A WN +YFV+LIV+GS+FM NL L V++
Sbjct: 1 MTAVRAIRVLRPLRAINRVPSIRILVTLLLDTLPMLGNVLAMCSLIFSIFGIVGVQMWQGVLRSRCVAQLPGNLSANQLNISAFYQPGGSSDLVCSLPENSGDKHCTSAFIPASTAHGRECGLTYEQYSNRSNYTNVCA-NWNQYYTLCADMGINPLYDAVSFDNILIAWVAIFQVITLEGWTDIMYYIQDA-HGFWNFIYFVVLIVIGSYFMTNLCLVVIT 220 HSP 6 Score: 80.4925 bits (197), Expect = 6.868e-15 Identity = 66/248 (26.61%), Postives = 110/248 (44.35%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDL-------RTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQT 946
R+ + +F+ + I N + +ALE H Q D K A +F +F EAILKI ALG Y+K WN LD ++V + ++ + L+ T++L R R +R+ R KL+ ++ +L ++ +A+ + +G+L L IF+ +G+E++ I C K G TC+ + NF + G AMLT+F+ T + W +L T
Sbjct: 1237 RKFIHAVCTHGYFDLGIAAVIALNVLCMALE-HYQQPDGLTAFLKCANY--VFTAVFILEAILKIFALGI----KRYIKDRWNQLDMLIVILSIVGIALEEMTMELPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELFGKI---DCEK-----------------------PGITCQGMDEH-----ANFRSFGIAMLTLFRISTGDNWNGILKDT 1446 HSP 7 Score: 77.411 bits (189), Expect = 5.015e-14 Identity = 75/243 (30.86%), Postives = 127/243 (52.26%), Query Frame = 2
Query: 1376 HFIKTQPFY-WGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIIN---PKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTL---------NFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSR-DFSM------IYSSYFLLIVIF 2044
H + T ++ G+ ++ LN C+A+EHY QP L FL A YVF ++F+ E +KIF GI+ Y + ++N D ++V +S+V + + P +R +R+ R+LKL + +R L+ ++ ++ + +L +L L FI + LG++LFG + KP + NF +F A++T+F I TG+NWN ++ + I + N D SM IY + F+L F
Sbjct: 1241 HAVCTHGYFDLGIAAVIALNVLCMALEHYQQPDGLTAFLKCANYVFTAVFILEAILKIFALGIKRYIKDRWNQLDMLIVILSIVGIALEEMTMELPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELFG---KIDCEKPGITCQGMDEHANFRSFGIAMLTLFRISTGDNWNGILKDTISPDVCRVNPEIDCSMLEHVAPIYFAVFVLATQF 1480 HSP 8 Score: 69.3218 bits (168), Expect = 1.594e-11 Identity = 56/225 (24.89%), Postives = 103/225 (45.78%), Query Frame = 2
Query: 3527 LKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGN-IELNDDTQINQNN--NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
L+ N FT IF E I+K+I F + KD +NIFD V+ S+ ++F + + LR FR++R+ K++ ++R L I + + L+ + F I+GM +FG +D + + + NF + ++ +F+ T E W+++M D G R S ++ YFI + + +++ NL VA++++ F
Sbjct: 443 LEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGDDDS---------GISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKYRFPNDEGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYD---GMRAT-------------------SKWAGLYFILLMTIGNYILFNLLVAILVEGF 636 HSP 9 Score: 68.5514 bits (166), Expect = 2.709e-11 Identity = 64/236 (27.12%), Postives = 116/236 (49.15%), Query Frame = 2
Query: 2393 RRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYAT 3100
R C + ++F IM IF +++ + E + + +LE F+ FT++F +EMI+K+I +G Y++D +NI D +VII+V F + + I LR R+LR K + +P L+ +I ++ NV L + ++F F ++ + LF GK+ + ND + + ET + N+D++ +++T+F V T E W V+ + + AT
Sbjct: 401 RDGCASSVDSKWFMYVIMGAIFVNTLTMGIEYYGQPQKMTD-VLEIFNYIFTAIFGIEMIMKLIGLGFY----GYIKDAFNIFDGTIVIISVVELFGDDD-SGISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKYRFPND--EGVMETSRA-----------------------NFDDLFWAIVTVFQVLTQEDWNIVMYDGMRAT 605
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Medaka
Match: cacna1c (voltage-dependent L-type calcium channel subunit alpha-1C [Source:NCBI gene;Acc:101173517]) HSP 1 Score: 1025.77 bits (2651), Expect = 0.000e+0 Identity = 670/1556 (43.06%), Postives = 948/1556 (60.93%), Query Frame = 2
Query: 194 LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQS--NTSCNA-GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKM----------RQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIE---------------------------ELNMY---DKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYS---SLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFND-QMGIQAKK-RIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLG--FLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
L + N IRR+C ++E + FE ++++TI ANCV+LA+ + P+ D + + +E +FL IFT EA LK+IA G + NAYL++ WN+LDFI+V +GL + L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC + + C P C G C+ W+GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A D +W +YFV LI++GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + +N EE + K R + E + + E C + K W + R + +K+Q FYW VI LV LNT +A EH+ QP++L A V L++F E+ +K++ G++ YF S FN FD VV ++E I G S LRC+RLLR+ K+TRYW+SL NLV SLL+S+RSI SLLLLL LFI I +LLGMQLFGG FNF++ + + FD FP +++TVF ILTGE+WN VMY+GI M M+ YF+++ I GNY LLNVFLAIAVDNLA+A L++A++EE + +E ++ ++ + +++ IE E N Y D D + E PV P I +++K +P + F+F N R +CH + N F I+ I SS++LAAEDPV +S RN++L + D FT +F +E+ILK+ G LH GS+ R+ +NI+D++VV +++ S S +N +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKFFYC D SK+ + C+G + + D +G + R+W+ + FN+D+++ M+ LF V+T EGWP +L +I + + GPI NY+ I+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YKVW +V S FEYL+ +I L++I L ++ H + T+N F ++ LN+ FT +FT E ILK+IA++ + +F DPWN+FDF+ V+GS+ DV LSE+ +++ E + F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I L D +QIN+NNNF+ F +LLLFRCATGE+W IM+ + C G+ S + CGS F+ YF++F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+WAE+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI + +R F K
Sbjct: 143 LLCLTLKNPIRRACISIVEWKPFEIIILMTIFANCVALAVYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKGDGATPIGGKAAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCVHNHTGLNAEEKPAPCAPDGAYGRHCTQNGTQCRVGWEGPNDGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVDYRWPWVYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPENEEEGLDDDKPRNLSMPASENESVNTDNPPGGDVEGETCCTRLAIRISKSKFSRYSRRWNRL---FRRKCRAAVKSQVFYWLVIFLVFLNTLTIASEHHRQPQWLTDVQDIANKVLLALFTGEMLLKMYSLGLQAYFVSLFNRFDSFVVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETRRS-TFDNFPQSLLTVFQILTGEDWNSVMYDGI---MAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEAKERKKLARLASPKKHEENEKPPLEEKKSEKIELKSITSDGETNPATKISMDDCCGDENEEKNPYPANDYIGDDDEEEPEMPVGPRPRPLSDIHLKEKAVPMPEARAFFVFSHTNKFRVLCHKIVNHNIFTNLILFFILLSSISLAAEDPVKNDSFRNQILGYADHVFTGLFTIEIILKMTAYGAFLHKGSFCRNYFNILDLVVVSVSLISSGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKFFYCTDSSKQTQAECRGSYIMYKDGDVGKPGRSPRVWENSDFNFDDVLQGMMALFAVSTFEGWPGLLYRAIDSHSEDVGPIYNYRVVISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKVWYVVNSTYFEYLMFTLILLNTICLAMQHHGQ-----------------TKN-FNDAMNILNMLFTGLFTVEMILKLIAFKPRGYFSDPWNVFDFLIVIGSIIDVILSEINGLQNSEENARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIALRDLSQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACSPNRPCEKGSTNEN----STASEECGSQFAIIYFVSFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIK--TDGNLEQANEELRAIVKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQEYFRKFKK 1659 HSP 2 Score: 98.9821 bits (245), Expect = 4.962e-20 Identity = 80/271 (29.52%), Postives = 138/271 (50.92%), Query Frame = 2
Query: 176 PNPD-RSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKP-----YWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQ 967
P P+ R+ F+FS N R C ++ F L++ I+ + +SLA E P + +F + + +F G+FT E ILK+ A G + K ++ ++ +NILD +VV++ LI+ +Q+ ++ ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F + TDS TQ C S G KP W+ F NFD++ M+ +F T EGW +LY +A DS
Sbjct: 908 PMPEARAFFVFSHTNKFRVLCHKIVNHNIFTNLILFFILLSSISLAAED--PVKNDSFRNQILGYADHVFTGLFTIEIILKMTAYGAFLHKGSFCRNYFNILDLVVVSVSLISSGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKFF---YCTDSS---KQTQAECR-GSYIMYKDGDVGKPGRSPRVWENSDF---NFDDVLQGMMALFAVSTFEGWPGLLY---RAIDSH 1163
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Medaka
Match: cacna1da (voltage-dependent L-type calcium channel subunit alpha-1D [Source:NCBI gene;Acc:101157531]) HSP 1 Score: 1019.22 bits (2634), Expect = 0.000e+0 Identity = 653/1538 (42.46%), Postives = 931/1538 (60.53%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNT-SCNA-GFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMK--------SEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D I + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ S + + C + C G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV LI+LGSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ + N +E DE + +AI W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S++ MI YF+++ I GNY LLNVFLAIAVDNLA+ ++ +D+ +EE +D P PVI + ++ ++KI P S+ FIF N +R CH + N F I++ I SS++LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K +E CKG + + D Q ++R+W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS ++ + + LN+ FT +FT E LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ S ++ + ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 75 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSINHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLELLTKEEPKVEGDGANNHPSMHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMPGSDLIAEEEPAPCAISGHGRQCPINGTECREGWHGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWVNDAIGFETPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDI----DPDNEDEA--------------------DEGSAAATLQSIKLINTLAAIRRWRRWNRFCRRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPLWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWQSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSST---GMIVCFYFIILFICGNYILLNVFLAIAVDNLADDE-----------------------------------EEFEDNAVEE-------EDPDVPSGPRPVISD------LVKKEKITPIPEGSAFFIFSNTNPVRVFCHKLINHHIFTNLILVFIMLSSVSLAAEDPIRNLSARNIILGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSSQEVCKGTYIVYKDGDVNQPTMQRRVWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG----------------QSALFNYVMDI--LNMVFTTVFTVEMFLKLIAFKPRHYFADAWNTFDALIVVGSVVDIAITEVNVSTCKNGGSLQADSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKLCDPESDYNIG-----EEMTCGSGFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1508 HSP 2 Score: 106.686 bits (265), Expect = 1.771e-22 Identity = 78/269 (29.00%), Postives = 133/269 (49.44%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N +R C LI F L+++ I+ + VSLA E + I+ + + F IFT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ TD ++Q+ C + N + W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 752 PIPEGSAFFIFSNTNPVRVFCHKLINHHIFTNLILVFIMLSSVSLAAEDPIRNLSARNII--LGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRC---TDEA---KSSQEVCKGTYIVYKDGDVNQPTMQRRVWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1006 HSP 3 Score: 72.4034 bits (176), Expect = 5.578e-12 Identity = 85/359 (23.68%), Postives = 144/359 (40.11%), Query Frame = 2
Query: 3299 NTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTE------IPNL--------GF----LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-GNIELN----------------------------DDTQINQ-----NN---NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFD 4195
N++P N N + LV PF+ I+I I + + L + + PE N L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + EE E P++ GF LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G + + T+ + NN NF NF+ +L +F+C T E W ++ G + + YF++ I++ +F +LNL + V+ F
Sbjct: 72 NSRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPE-------------DDSNSINHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLELLTKEEPKVEGDGANNHPSMHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMPGSDLIAEEEPAPCAISGHGRQCPINGTECREGWHGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWVNDA---------------------IGFETPWIYFVSLIILGSFFVLNLVLGVLSGEFS 394
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Medaka
Match: cacna1da (voltage-dependent L-type calcium channel subunit alpha-1D [Source:NCBI gene;Acc:101157531]) HSP 1 Score: 1018.45 bits (2632), Expect = 0.000e+0 Identity = 656/1540 (42.60%), Postives = 933/1540 (60.58%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSN-TSCN-AGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWL--ERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSE--------MKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D I + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ S + + C + C G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE+ I +N+ A+E K E + E + +DE W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S++ MI YF+++ I GNY LLNVFLAIAVDN E + + D+++ EE D P PVI + ++ ++KI P S+ FIF N +R CH + N F I++ I SS++LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K +E CKG + + D Q ++R+W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS ++ + + LN+ FT +FT E LK+IA++ + +F D WN+FD + V+GS+ D+ LSE +++ E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 123 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSINHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLELLTKEEPKVEGDGANNHPSMHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMPGSDLIAEEEPAPCAISGHGRQCPINGTECREGWHGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAED-IDPDNEDEADEGKRNPSVPASETESVNTENQNGEDE----------KTPCCGPLCRRWRRWNRFCRRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPLWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWQSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSST---GMIVCFYFIILFICGNYILLNVFLAIAVDN---------------------------------LADEDEEEFEDNAVEEE--------DPDVPSGPRPVISD------LVKKEKITPIPEGSAFFIFSNTNPVRVFCHKLINHHIFTNLILVFIMLSSVSLAAEDPIRNLSARNIILGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSSQEVCKGTYIVYKDGDVNQPTMQRRVWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG----------------QSALFNYVMDI--LNMVFTTVFTVEMFLKLIAFKPRGYFGDAWNVFDALVVIGSIVDIVLSEIDVSNPLSLQNTEDSARI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKLCDPESDYNIG-----EEMTCGSGFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1571 HSP 2 Score: 106.686 bits (265), Expect = 1.991e-22 Identity = 77/269 (28.62%), Postives = 132/269 (49.07%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N +R C LI F L+++ I+ + VSLA E + I+ + + F IFT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S +Q+ C + N + W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 816 PIPEGSAFFIFSNTNPVRVFCHKLINHHIFTNLILVFIMLSSVSLAAEDPIRNLSARNII--LGYFDYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKS------SQEVCKGTYIVYKDGDVNQPTMQRRVWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1070 HSP 3 Score: 72.4034 bits (176), Expect = 4.875e-12 Identity = 85/358 (23.74%), Postives = 144/358 (40.22%), Query Frame = 2
Query: 3299 NTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTE------IPNL--------GF----LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-GNIELN----------------------------DDTQINQ-----NN---NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
N++P N N + LV PF+ I+I I + + L + + PE N L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + EE E P++ GF LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G + + T+ + NN NF NF+ +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 120 NSRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPE-------------DDSNSINHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLELLTKEEPKVEGDGANNHPSMHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMPGSDLIAEEEPAPCAISGHGRQCPINGTECREGWHGPNNGITNFDNFLFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 441
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Medaka
Match: cacna1da (voltage-dependent L-type calcium channel subunit alpha-1D [Source:NCBI gene;Acc:101157531]) HSP 1 Score: 1015.76 bits (2625), Expect = 0.000e+0 Identity = 659/1534 (42.96%), Postives = 942/1534 (61.41%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT--------------------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSN-TSCN-AGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWL--ERKMRQDEYI--CXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLN-FDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQA--KKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ +++ I ANC++LA+ + P+ D I + +E FL IFT E LKIIA G ++ +NAY+++ WN+LDF++V IGL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ S + + C + C G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE+ I +N+ A+E K E + E + +DE C K W ++ R + +K+ PFYW VI LV LNT ++ EHY QP +L + A V L++F E+ +K++ G++ YF S FN FD VV + E I + G S RC+RLLR+ K+TR+W SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQ+FGG FNF++ + + FD FP A++TVF ILTGE+WN VMY+GI + S++ MI YF+++ I GNY LLNVFLAIAVDNLA+A L+ E ++ ++ ++ ++EE + E + D+ P PVI + ++ ++KI P S+ FIF N +R CH + N F I++ I SS++LAAEDP+ S RN +L + D FTS+F E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K +E CKG + + D Q ++R+W + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L ++ + QS ++ + + LN+ FT +FT E LK+IA++ + +F D WN FD + V+GS+ D+ ++E+ + E + I ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +LLLFRCATGE+W IM+ GK C + G ++ CGS F+ YFITF ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + ++ ++ELR + K+W + + +D +P +TVGK YA LI +R F K
Sbjct: 75 PQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAVYIPFPEDDSNSINHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLELLTKEEPKVEGDGANNHPSMHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMPGSDLIAEEEPAPCAISGHGRQCPINGTECREGWHGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAED-IDPDNEDEADEGKRNPSVPASETESVNTENQNGEDEKTPCCGPLCQKISKSKFSRRWRRWNRFC---RRKCRLAVKSVPFYWLVIILVFLNTLTISSEHYNQPLWLTQVQDVANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWQSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQVFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSST---GMIVCFYFIILFICGNYILLNVFLAIAVDNLADAESLNTDEGDKKGDKKDDEKDEKDEEEFEDNAVEEEDPDV-------------------PSGPRPVISD------LVKKEKITPIPEGSAFFIFSNTNPVRVFCHKLINHHIFTNLILVFIMLSSVSLAAEDPIRNLSARNIVLGYADYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSSQEVCKGTYIVYKDGDVNQPTMQRRVWHNSDFNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENFGPIYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAVQHYG----------------QSALFNYVMDI--LNMVFTTVFTVEMFLKLIAFKPRHYFADAWNTFDALIVVGSVVDIAITEVNNTEDSARI-SITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKLCDPESDYNIG-----EEMTCGSGFAIVYFITFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLEQANEELRAVIKKIWKRTSMK-LLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKK 1546 HSP 2 Score: 107.457 bits (267), Expect = 1.185e-22 Identity = 78/269 (29.00%), Postives = 133/269 (49.44%), Query Frame = 2
Query: 176 PNPDRS-LFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
P P+ S FIFS N +R C LI F L+++ I+ + VSLA E + IV A + +F +FT E +LK+ G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ + S +Q+ C + N + W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 799 PIPEGSAFFIFSNTNPVRVFCHKLINHHIFTNLILVFIMLSSVSLAAEDPIRNLSARNIVLGYA--DYVFTSMFTFEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKS------SQEVCKGTYIVYKDGDVNQPTMQRRVWHNSDF---NFDNVLMAMMALFTVSTFEGWPALLY---KAIDS 1053 HSP 3 Score: 72.7886 bits (177), Expect = 4.248e-12 Identity = 85/358 (23.74%), Postives = 144/358 (40.22%), Query Frame = 2
Query: 3299 NTKPTSRYMPAN-KNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKL----FFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTE------IPNL--------GF----LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIF-GNIELN----------------------------DDTQINQ-----NN---NFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
N++P N N + LV PF+ I+I I + + L + + PE N L+ + AF IIFT E LK+IAY + + ++ WN+ DFV V+ L V L + EE E P++ GF LR FRV+R ++++ S++++L + I++ L + LL+ + IY IIG+++F G + + T+ + NN NF NF+ +L +F+C T E W + L + G + + YF++ ++ +F +LNL + V+ F
Sbjct: 72 NSRPQRALFCLNLNNPIRRACISLVEWKPFDIFILIAIFANCMALAV--YIPFPE-------------DDSNSINHDLETVEYAFLIIFTIETFLKIIAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLELLTKEEPKVEGDGANNHPSMHGHGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYMPGSDLIAEEEPAPCAISGHGRQCPINGTECREGWHGPNNGITNFDNFLFAMLTVFQCITMEGWTDV---------------------LYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEF 393
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Medaka
Match: CACNA1D (calcium voltage-gated channel subunit alpha1 D [Source:NCBI gene;Acc:101171831]) HSP 1 Score: 1013.06 bits (2618), Expect = 0.000e+0 Identity = 655/1524 (42.98%), Postives = 933/1524 (61.22%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADT---------------VDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACY--PQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQG-KPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDD--SIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPY---SSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSF-FYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYF--YTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIP---NLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNK 4666
P R+LF + NN IRR+C L+E + F+ ++L+I ANCV+LA+ + P D ++ +E FL IFT E +KIIA G ++ +N+Y+++ WN+LDF++V +GL +V L+ T D++ RA RVLRP +LVSGVPSLQVVL SIIKAM PLL I LLVLF ++I+AIIGLE++ G H TC+ + L + C G C+ W GP G+ NFDN +AMLTVFQCITMEGWTDVLY N A + +YFV L++ GSFF+LNLVLGVLSGEF+KERE+ + R F KLRE Q+ E L GY++W+ AE++ +++ EE K RK RQ W ++ R + +K+ FYW VI LV LNT +A EHY QP +L A V L++F E+ +K++ G++ YF S FN FD VV +VE I + G S RC+RLLR+ K+TR+W+SL NLV SLL+SM+SI SLLLLL LFI I +LLGMQLFGG FNF++ FD FP A++TVF ILTGE+WN VMY+GI + ++S M+ YF+++ I GNY LLNVFLAIAVDNLA+A L+ A+ EE + ++ R + +E ++ + + E +D + E P + +++K P S+ FIF N R +CH + N + F I++ I SS++LAAEDP+ S RN +L +FD AFT++F +E++LK+ G LH G++ R+ +N++D++VV +++ SF S ++ +K LRV+RVLRPL+ INR LK V + + +++ + NI+IV L QF+F I VQLF GKF+ C D +K E CKG + Y+ D K+RIW + FN+DN++ +M+ LFTV+T EGWP +L +I + N GPI NY+ EI+IF+I++ I+ FF +NIFV +IVTFQEQGE + + ELDKN+ +C+++A+ +P RY+P KN YK W +V S FEY++ ++I L+++ L I+ + + + F ++ LN+ FT +FT E ILK+IA++ + + D WN+FD + V+GS+ D+ LS++ E TE ++ F RLFRVMRL+K+L RG IR LLWTFI+SF+ALP+V LLI +LFFIY +IGMQ+FG I + D TQIN+NNNF+ F +L+LFRCATGE+W IM+ GK C + G +++ CGS F+ YFI+F ++ FL++NLFVAVIMDNFDYLTRD SILGPHHLDE+ R+W+E+DP G + D+ +++++ PP+GFGK CP+R AC +L+ MNM L++ V FN+TLFAL+R +L+IK + +D ++ELR+ + K+W +D +P +TVGK YA LI +R F +
Sbjct: 21 PPRALFCLTLNNPIRRACISLVEWKPFDIFILLSIFANCVALAIYIPFPGDDSNSTNQELEKVEYAFLIIFTIETFMKIIAYGLVMHQNSYVRNGWNMLDFVIVIVGLFSVVLEMITKDADHGGQSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHATCYVIQTGALAEEEPAPCAVSGHGRHCLFNGTICREGWQGPNNGITNFDNFLFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEGDEEGKR------------NRKFRQ-----------------------WRRWNRLCRRKCRAAVKSVTFYWLVIILVFLNTLTIASEHYNQPDWLTEVQDVANKVLLALFTLEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELAIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETVTKRSTFDNFPQALLTVFQILTGEDWNTVMYDGIMAYGGPASS---GMVVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQREEEEAKKRRNSTNVHPLLTVQLPTETLLEADKEPYAAAESPGDDVDDEDNNDILPEVPTGPRPQRLSELTIKEKTPPIPQGSAFFIFSSTNPFRVLCHKLINHQIFTNLILVFIMLSSVSLAAEDPIRNFSARNIILGYFDYAFTAIFTVEILLKMTAFGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDDAKSSPEECKGTYILYSNGDTALPMIKERIWYNSDFNFDNVLMAMMALFTVSTFEGWPTLLYKAIDSNRENMGPIYNYRIEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP--KNPYQYKFWYVVNSTGFEYVMFVLIILNTLCLAIQHYGQ------------------SHLFNYAMDILNMVFTGVFTVEMILKLIAFKPRGYVGDAWNVFDALVVIGSVVDIILSQVIVEPGNTEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDGTQINRNNNFQTFPQAVLMLFRCATGEAWQEIMLACLPGKLCDSESDYNPG-----EERTCGSGFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIK--TEGNLDQANEELRVVIKKIWKR-TSMKLLDQVVP-PAGDDEVTVGKFYATFLIQDYFRKFKR 1477 HSP 2 Score: 103.99 bits (258), Expect = 1.304e-21 Identity = 81/282 (28.72%), Postives = 137/282 (48.58%), Query Frame = 2
Query: 143 QTLSISFVGQKPNP---DRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD-LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQ----SNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
Q LS + +K P + FIFS N R C LI + F L+++ I+ + VSLA E + I+ + + F IFT E +LK+ A G + K A+ ++ +N+LD +VV + L++ +Q+ + ++ R +RVLRP + ++ L+ V+ + A+ + I ++ +FA IG+++++G F+ TD ++ + C SN + W F NFDN+ AM+ +F T EGW +LY KA DS
Sbjct: 713 QRLSELTIKEKTPPIPQGSAFFIFSSTNPFRVLCHKLINHQIFTNLILVFIMLSSVSLAAEDPIRNFSARNII--LGYFDYAFTAIFTVEILLKMTAFGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRC---TDDA---KSSPEECKGTYILYSNGDTALPMIKERIWYNSDF---NFDNVLMAMMALFTVSTFEGWPTLLY---KAIDS 980
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Planmine SMEST
Match: SMESG000019762.1 (SMESG000019762.1) HSP 1 Score: 2794.99 bits (7244), Expect = 0.000e+0 Identity = 1563/1563 (100.00%), Postives = 1563/1563 (100.00%), Query Frame = 2
Query: 359 MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNKWXXXXXXXXXXXXXXQYYFPSYKDYRIMDSERXXXXXXXXXXXXXXXXENEVQSSDDVFLGEDADQQDIKELVEDSDESPVIIDSIHDDVDASRRPGLFPNTVSEIFVKVQQLIVDSKNKWIGDNDK 5047
MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICELESADDLDDKSKSAIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSALRCIRLLRLLKLTRYWSSLRNLVFSLLSSMRSIISLLLLLGLFIFILTLLGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDNLANAAELSAAEEEETKKEELRREEAENEERRKLFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKNVFNILIVFILFQFIFGVIAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAIFYIVFFIVFPFFFVNIFVALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNKWRNLSTEERLSRRSSQYYFPSYKDYRIMDSERYYYSSSLLSNQYYHNYENEVQSSDDVFLGEDADQQDIKELVEDSDESPVIIDSIHDDVDASRRPGLFPNTVSEIFVKVQQLIVDSKNKWIGDNDK
Sbjct: 1 MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICELESADDLDDKSKSAIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSALRCIRLLRLLKLTRYWSSLRNLVFSLLSSMRSIISLLLLLGLFIFILTLLGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDNLANAAELSAAEEEETKKEELRREEAENEERRKLFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVIVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKNVFNILIVFILFQFIFGVIAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAIFYIVFFIVFPFFFVNIFVALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNKWRNLSTEERLSRRSSQYYFPSYKDYRIMDSERYYYSSSLLSNQYYHNYENEVQSSDDVFLGEDADQQDIKELVEDSDESPVIIDSIHDDVDASRRPGLFPNTVSEIFVKVQQLIVDSKNKWIGDNDK 1563 HSP 2 Score: 118.242 bits (295), Expect = 3.584e-26 Identity = 81/267 (30.34%), Postives = 134/267 (50.19%), Query Frame = 2
Query: 164 VGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
V +K P SLFIF +N IRR C + R+F+ +++ I A+ ++LA E P + + + F +F E ILK+I +G ++ +Y++ WNI+D IVV I + + + +++ R IRVLRP K ++ VP L+ V +II ++ + I ++ + IF +I ++++ G F + D T + + N K W +F N+DNI +M+T+F T EGW DVL + AT S
Sbjct: 659 VRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKNVFNILIVFILFQFIFGVIAVQLFSGKFF---YCNDLSKRIKETCKGYFYTFNDQMGIQ-AKKRIWQRNYF---NYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYS 916
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Planmine SMEST
Match: SMESG000019762.1 (SMESG000019762.1) HSP 1 Score: 2789.22 bits (7229), Expect = 0.000e+0 Identity = 1562/1564 (99.87%), Postives = 1563/1564 (99.94%), Query Frame = 2
Query: 359 MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPV-IVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNKWXXXXXXXXXXXXXXQYYFPSYKDYRIMDSERXXXXXXXXXXXXXXXXENEVQSSDDVFLGEDADQQDIKELVEDSDESPVIIDSIHDDVDASRRPGLFPNTVSEIFVKVQQLIVDSKNKWIGDNDK 5047
MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICELESADDLDDKSKSAIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSALRCIRLLRLLKLTRYWSSLRNLVFSLLSSMRSIISLLLLLGLFIFILTLLGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDNLANAAELSAAEEEETKKEELRREEAENEERRKLFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPV +VENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKNVFNILIVFILFQFIFGVIAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAIFYIVFFIVFPFFFVNIFVALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNKWRNLSTEERLSRRSSQYYFPSYKDYRIMDSERYYYSSSLLSNQYYHNYENEVQSSDDVFLGEDADQQDIKELVEDSDESPVIIDSIHDDVDASRRPGLFPNTVSEIFVKVQQLIVDSKNKWIGDNDK
Sbjct: 1 MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICELESADDLDDKSKSAIENWIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSALRCIRLLRLLKLTRYWSSLRNLVFSLLSSMRSIISLLLLLGLFIFILTLLGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVDNLANAAELSAAEEEETKKEELRREEAENEERRKLFGSEAKIQDIDDSIIEELNMYDKPKDKKQPIKESPVKVVENPPAEGIIVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKNVFNILIVFILFQFIFGVIAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAIFYIVFFIVFPFFFVNIFVALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKASYPLTVGKIYAGMLIMKNWRNFNKWRNLSTEERLSRRSSQYYFPSYKDYRIMDSERYYYSSSLLSNQYYHNYENEVQSSDDVFLGEDADQQDIKELVEDSDESPVIIDSIHDDVDASRRPGLFPNTVSEIFVKVQQLIVDSKNKWIGDNDK 1564 HSP 2 Score: 118.627 bits (296), Expect = 3.028e-26 Identity = 81/267 (30.34%), Postives = 134/267 (50.19%), Query Frame = 2
Query: 164 VGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDS 964
V +K P SLFIF +N IRR C + R+F+ +++ I A+ ++LA E P + + + F +F E ILK+I +G ++ +Y++ WNI+D IVV I + + + +++ R IRVLRP K ++ VP L+ V +II ++ + I ++ + IF +I ++++ G F + D T + + N K W +F N+DNI +M+T+F T EGW DVL + AT S
Sbjct: 660 VRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAED--PVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKNVFNILIVFILFQFIFGVIAVQLFSGKFF---YCNDLSKRIKETCKGYFYTFNDQMGIQ-AKKRIWQRNYF---NYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYS 917
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1) HSP 1 Score: 875.93 bits (2262), Expect = 0.000e+0 Identity = 474/794 (59.70%), Postives = 607/794 (76.45%), Query Frame = 2
Query: 2330 IVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY---------------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKAS--YPLTVGKIYAGMLIMKNWRNF 4660
I RK +LPYS++FIF P N +RR CHFV NLR+FD+FIM+VI ASS+ALAAEDPV SL+N +LEFFD AFT VF +EMILK+ID+G++LHPGSY RD WNI+D VVI A+ +F +KNLNTIKSLRV+RVLRPLKTINRVPKLKAVF+ ++SS+KNVFNILIV+ILFQFIF VIAVQLF GKFFYCND SK +ETC+GYF+ + + + KKR+WQ + F++DNI+ +M+TLFTVTTGEGWP VLKNS+ +T + GPI +Y+QE+AIFYIVFFIVFPFFFVNIFVALII+TFQEQGE++ DL+LDKN+ +CIDFAIN KP RYMP +K ++ Y+VW LVVS PFEY IM++IAL+++ L++K + + E+ S REQ ++C +L +LN AFT++F+ EC+LK++A+ K +F+DPWNIFDF+TV+GS+TDV +SE++ + +LGFLRLFR RLIK+L +GY+IRILLWTFIQSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + QIN++NNF+ F GLLLLFRCATGESW IM+ ++ K C T CGS+F+Y YF++FI + +FLMLNLFVAVIMDNFDYLTRD SILGPHHLDEY+R+WAE+DP G ++ +MY+M++ + PPVGFGK CPYR A KLIRMNM +D VNF +TLFALIRESL IKM + +D D ELR + K+WP + + +++ +P + + +TVGKIYAG LI++NWR +
Sbjct: 740 IQRKPVLPYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAEDPVKEKSLKNNILEFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKFFYCNDASKSSRETCRGYFFDYAGRSTPETKKRLWQHHDFHFDNILYAMLTLFTVTTGEGWPTVLKNSMESTNIDNGPIADYRQEMAIFYIVFFIVFPFFFVNIFVALIIITFQEQGENELIDLDLDKNQKQCIDFAINEKPLCRYMPKDKTTIKYRVWRLVVSTPFEYFIMVMIALNTLILMMKYYRE--EKKASSAATHDREQQAYLNYCNTLIFLNSAFTVMFSIECVLKIMAFGPKNYFRDPWNIFDFITVIGSITDVLVSELQD----SSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVFGNIKLISEYQINRHNNFQTFTNGLLLLFRCATGESWQEIMLACDNNKPCEKDIENTNNFD-------CGSNFTYIYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEYIRVWAEYDPGAIGRIHHSEMYEMLRNMEPPVGFGKKCPYRLAYRKLIRMNMPVDENGTVNFTTTLFALIRESLNIKMSTAEYMDIKDMELRQTICKMWP-VQAKKKLNLIVPPDSELVFHKMTVGKIYAGYLIVENWRAY 1519 HSP 2 Score: 543.117 bits (1398), Expect = 7.029e-161 Identity = 340/625 (54.40%), Postives = 433/625 (69.28%), Query Frame = 2
Query: 311 LEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLF--DATQQACYPQSNT---------SCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN---------------------WIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
+E HLP+ D+T + ++ E FLGIF EA+LKI+ALGF K AYL++VWNI+DF+VV GLI+ L + D R RA+RVLRP KLV+G SLQVVL SII+AMAPLLQIGLLVLFA+ IFAIIGLE Y G FH TC+K +S L T+++ P SN+ +C + CK YW+GP +G+ +FDNIGYAMLTVFQCITMEGWTD++Y TN A +++N LYFV LI+LGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLR Q+ E +GYM W++ AEE++ E +E+K ++ R++ ++ + + +++ I + E D +++ W H R V+ +K+QPFYW VI LV NT CVA+EHY Q K+L FL YAEYVFL +FM E+ IK++G IR YF+S FN+FD +V+ SL E I+ + SFG S LR +RLLR+ K+TRYW+SLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG+FNFE+GKP NFDTF A++TVF ILTGE+WN VMYNGI+S+ ++ ++S YF+L+V+FGNYTLLNVFLAIAVD
Sbjct: 1 MERHLPKKDKTLLAGQLELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPS--FDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCFKANSDKLPLPIPTEKSLVPCSNSKNGSGLGAYTCPEDYQCKAYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIMYHTNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNEATTTSEDKMRIIAARRRAEKQKYKLLGKNQSIPDFEYEDTELYSETKSMKGNIFIRSYAGIRRRRKERCTSFWRTEKH-FRFSVRRLVKSQPFYWAVIILVFFNTVCVAIEHYEQGKWLTLFLNYAEYVFLCLFMMEMMIKMYGLSIRVYFQSSFNIFDCVVIAGSLFEIIYGLFVQDSSFGLSVLRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFNFEEGKPPQNFDTFVKALLTVFQILTGEDWNTVMYNGIQSK---GGVKERGTVFSIYFVLLVVFGNYTLLNVFLAIAVD 619 HSP 3 Score: 123.635 bits (309), Expect = 9.345e-28 Identity = 83/281 (29.54%), Postives = 142/281 (50.53%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV-----------NLQADTVD----LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIF------HNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD 961
P ++FIFS N +RR C ++ R+F+ +++ I A+ ++LA E P +++ + + F G+FT E ILKII LG I+ +Y + +WNILD VV L+ L+ + +++ R +RVLRP K ++ VP L+ V ++ ++ + I ++ + IFA+I +++++G F + +T + FD ++ P+ T K W F +FDNI YAMLT+F T EGW VL + ++T+
Sbjct: 747 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAED--PVKEKSLKNNILEFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKFFYCNDASKSSRETCRGYFFDYAGRST-PE---------TKKRLWQHHDF---HFDNILYAMLTLFTVTTGEGWPTVLKNSMESTN 1012 HSP 4 Score: 83.1889 bits (204), Expect = 1.800e-15 Identity = 76/283 (26.86%), Postives = 136/283 (48.06%), Query Frame = 2
Query: 3350 YKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFG---NIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDN 4189
+ V LV S PF + ++I++ +++ + I+ + + K+L L L Y F +F E ++KM +++F+ +NIFD V + GSL ++ + S L LR R++R+ KV S+R L+ + + S +++ + L+ L I+ ++GMQ+FG N E Q NF F+ LL +F+ TGE W+ +M + G + GG+ K G+ FS YF+ ++ + +LN+F+A+ +DN
Sbjct: 381 FSVRRLVKSQPFYWAVIILVFFNTVCVAIEHYEQ---------GKWL---------TLFLNYAEYVFLCLFMMEMMIKMYGLSIRVYFQSSFNIFDCVVIAGSLFEIIYGLFVQDSSF----GLSVLRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFNFEEGKPPQ-----NFDTFVKALLTVFQILTGEDWNTVMYN---------GIQSKGGV------KERGTVFSI-YFVLLVVFGNYTLLNVFLAIAVDN 620 HSP 5 Score: 76.6406 bits (187), Expect = 2.183e-13 Identity = 78/281 (27.76%), Postives = 135/281 (48.04%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVN----LQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY---QTNKATDSQWNV--LYFVLLIVLGSFFMLNLVLGV 1039
R S + L++ + F + VI+ + N V +A+E H QG + A E +FL +F E ++K+ L V Y +S +NI D +V+ L + +Q + L RA+R+LR FK+ SL+ ++ S++ +M ++ + L+ +LIFA++G++++ G F+ ++ PQ NFD A+LTVFQ +T E W V+Y Q+ + V +YFVLL+V G++ +LN+ L +
Sbjct: 380 RFSVRRLVKSQPFYWAVIILVFFNTVCVAIE-HYEQGKWLTLFLNYA--EYVFLCLFMMEMMIKMYGLSIRV----YFQSSFNIFDCVVIAGSLFEIIYGLFVQDSSFGLSVLRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFN--------------FEEGKPPQ-----------------------NFDTFVKALLTVFQILTGEDWNTVMYNGIQSKGGVKERGTVFSIYFVLLVVFGNYTLLNVFLAI 616 HSP 6 Score: 75.0998 bits (183), Expect = 5.651e-13 Identity = 60/204 (29.41%), Postives = 99/204 (48.53%), Query Frame = 2
Query: 2522 LEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIK-----ETCKGYFYTFNDQMG-----------IQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
LE + F +F +E +LK++ +G H +Y+R+ WNIMD +VV+ + S ++LR +RVLRPLK + L+ V ++II ++ + I ++ + IF +I ++ +SG F Y + K T K N + G Q K NY ++DNI +M+T+F T EGW D++
Sbjct: 17 LELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPS--FDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCFKANSDKLPLPIPTEKSLVPCSNSKNGSGLGAYTCPEDYQCKAYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIM 218 HSP 7 Score: 68.1662 bits (165), Expect = 6.564e-11 Identity = 75/296 (25.34%), Postives = 124/296 (41.89%), Query Frame = 2
Query: 260 FEYLVILTIIANCVSLALEVHLPQGDRTFIVTK-------------MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD--------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY--QTNKATD------------SQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
FEY +++ I N + L ++ + + + T + + F +F+ E +LKI+A G KN Y + WNI DFI V IG IT L ++ D LR FRA R++ KL+ +++++L + I++ L + LL+ I++IIG++V+ I K S + + NF +L +F+C T E W +++ NK + S + +YFV I L SF MLNL + V+
Sbjct: 1106 FEYFIMVMIALNTLILMMKYYREEKKASSAATHDREQQAYLNYCNTLIFLNSAFTVMFSIECVLKIMAFG---PKN-YFRDPWNIFDFITV-IGSITDVLVSELQDSSFLSLGFLRLFRAARLI---KLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVFGNI------KLISEYQINRHN-----------------------------NFQTFTNGLLLLFRCATGESWQEIMLACDNNKPCEKDIENTNNFDCGSNFTYIYFVSFIFLCSFLMLNLFVAVI 1358
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1) HSP 1 Score: 875.93 bits (2262), Expect = 0.000e+0 Identity = 474/794 (59.70%), Postives = 607/794 (76.45%), Query Frame = 2
Query: 2330 IVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY---------------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKAS--YPLTVGKIYAGMLIMKNWRNF 4660
I RK +LPYS++FIF P N +RR CHFV NLR+FD+FIM+VI ASS+ALAAEDPV SL+N +LEFFD AFT VF +EMILK+ID+G++LHPGSY RD WNI+D VVI A+ +F +KNLNTIKSLRV+RVLRPLKTINRVPKLKAVF+ ++SS+KNVFNILIV+ILFQFIF VIAVQLF GKFFYCND SK +ETC+GYF+ + + + KKR+WQ + F++DNI+ +M+TLFTVTTGEGWP VLKNS+ +T + GPI +Y+QE+AIFYIVFFIVFPFFFVNIFVALII+TFQEQGE++ DL+LDKN+ +CIDFAIN KP RYMP +K ++ Y+VW LVVS PFEY IM++IAL+++ L++K + + E+ S REQ ++C +L +LN AFT++F+ EC+LK++A+ K +F+DPWNIFDF+TV+GS+TDV +SE++ + +LGFLRLFR RLIK+L +GY+IRILLWTFIQSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + QIN++NNF+ F GLLLLFRCATGESW IM+ ++ K C T CGS+F+Y YF++FI + +FLMLNLFVAVIMDNFDYLTRD SILGPHHLDEY+R+WAE+DP G ++ +MY+M++ + PPVGFGK CPYR A KLIRMNM +D VNF +TLFALIRESL IKM + +D D ELR + K+WP + + +++ +P + + +TVGKIYAG LI++NWR +
Sbjct: 813 IQRKPVLPYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAEDPVKEKSLKNNILEFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKFFYCNDASKSSRETCRGYFFDYAGRSTPETKKRLWQHHDFHFDNILYAMLTLFTVTTGEGWPTVLKNSMESTNIDNGPIADYRQEMAIFYIVFFIVFPFFFVNIFVALIIITFQEQGENELIDLDLDKNQKQCIDFAINEKPLCRYMPKDKTTIKYRVWRLVVSTPFEYFIMVMIALNTLILMMKYYRE--EKKASSAATHDREQQAYLNYCNTLIFLNSAFTVMFSIECVLKIMAFGPKNYFRDPWNIFDFITVIGSITDVLVSELQD----SSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVFGNIKLISEYQINRHNNFQTFTNGLLLLFRCATGESWQEIMLACDNNKPCEKDIENTNNFD-------CGSNFTYIYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEYIRVWAEYDPGAIGRIHHSEMYEMLRNMEPPVGFGKKCPYRLAYRKLIRMNMPVDENGTVNFTTTLFALIRESLNIKMSTAEYMDIKDMELRQTICKMWP-VQAKKKLNLIVPPDSELVFHKMTVGKIYAGYLIVENWRAY 1592 HSP 2 Score: 515.383 bits (1326), Expect = 6.630e-151 Identity = 327/612 (53.43%), Postives = 417/612 (68.14%), Query Frame = 2
Query: 311 LEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLF--DATQQACYPQSNT---------SCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN---------------------WIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGN 2050
+E HLP+ D+T + ++ E FLGIF EA+LKI+ALGF K AYL++VWNI+DF+VV GLI+ L + D R RA+RVLRP KLV+G SLQVVL SII+AMAPLLQIGLLVLFA+ IFAIIGLE Y G FH TC+K +S L T+++ P SN+ +C + CK YW+GP +G+ +FDNIGYAMLTVFQCITMEGWTD++Y TN A +++N LYFV LI+LGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLR Q+ E +GYM W++ AEE++ E +E+K ++ R++ ++ + + +++ I + E D +++ W H R V+ +K+QPFYW VI LV NT CVA+EHY Q K+L FL YAEYVFL +FM E+ IK++G IR YF+S FN+FD +V+ SL E I+ + SFG S LR +RLLR+ K+TRYW+SLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG+FNFE+GKP NFDTF A++TVF ILTGE+WN MY+GI+++ SM S+YF LI+IFGN
Sbjct: 1 MERHLPKKDKTLLAGQLELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLP--SFDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCFKANSDKLPLPIPTEKSLVPCSNSKNGSGLGAYTCPEDYQCKAYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIMYHTNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNEATTTSEDKMRIIAARRRAEKQKYKLLGKNQSIPDFEYEDTELYSETKSMKGNIFIRSYAGIRRRRKERCTSFWRTEKH-FRFSVRRLVKSQPFYWAVIILVFFNTVCVAIEHYEQGKWLTLFLNYAEYVFLCLFMMEMMIKMYGLSIRVYFQSSFNIFDCVVIAGSLFEIIYGLFVQDSSFGLSVLRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFNFEEGKPPQNFDTFVKALLTVFQILTGEDWNTAMYSGIKAKK--------SMAASAYFALIMIFGN 601 HSP 3 Score: 123.635 bits (309), Expect = 1.040e-27 Identity = 83/281 (29.54%), Postives = 142/281 (50.53%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV-----------NLQADTVD----LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIF------HNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD 961
P ++FIFS N +RR C ++ R+F+ +++ I A+ ++LA E P +++ + + F G+FT E ILKII LG I+ +Y + +WNILD VV L+ L+ + +++ R +RVLRP K ++ VP L+ V ++ ++ + I ++ + IFA+I +++++G F + +T + FD ++ P+ T K W F +FDNI YAMLT+F T EGW VL + ++T+
Sbjct: 820 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAED--PVKEKSLKNNILEFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKFFYCNDASKSSRETCRGYFFDYAGRST-PE---------TKKRLWQHHDF---HFDNILYAMLTLFTVTTGEGWPTVLKNSMESTN 1085 HSP 4 Score: 75.0998 bits (183), Expect = 5.458e-13 Identity = 60/204 (29.41%), Postives = 99/204 (48.53%), Query Frame = 2
Query: 2522 LEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIK-----ETCKGYFYTFNDQMG-----------IQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
LE + F +F +E +LK++ +G H +Y+R+ WNIMD +VV+ + S ++LR +RVLRPLK + L+ V ++II ++ + I ++ + IF +I ++ +SG F Y + K T K N + G Q K NY ++DNI +M+T+F T EGW D++
Sbjct: 17 LELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPS--FDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCFKANSDKLPLPIPTEKSLVPCSNSKNGSGLGAYTCPEDYQCKAYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIM 218 HSP 5 Score: 72.7886 bits (177), Expect = 3.332e-12 Identity = 38/66 (57.58%), Postives = 49/66 (74.24%), Query Frame = 2
Query: 1904 IITVFV----ILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
I +VF ILTGE+WN VMYNGI+S+ ++ ++S YF+L+V+FGNYTLLNVFLAIAVD
Sbjct: 630 IFSVFACHCKILTGEDWNTVMYNGIQSK---GGVKERGTVFSIYFVLLVVFGNYTLLNVFLAIAVD 692 HSP 6 Score: 70.8626 bits (172), Expect = 1.259e-11 Identity = 72/266 (27.07%), Postives = 124/266 (46.62%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVN----LQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGS 1009
R S + L++ + F + VI+ + N V +A+E H QG + A E +FL +F E ++K+ L V Y +S +NI D +V+ L + +Q + L RA+R+LR FK+ SL+ ++ S++ +M ++ + L+ +LIFA++G++++ G F+ ++ PQ NFD A+LTVFQ +T E W +Y KA S YF L+++ G+
Sbjct: 380 RFSVRRLVKSQPFYWAVIILVFFNTVCVAIE-HYEQGKWLTLFLNYA--EYVFLCLFMMEMMIKMYGLSIRV----YFQSSFNIFDCVVIAGSLFEIIYGLFVQDSSFGLSVLRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFN--------------FEEGKPPQ-----------------------NFDTFVKALLTVFQILTGEDWNTAMYSGIKAKKSMAASAYFALIMIFGN 601 HSP 7 Score: 68.9366 bits (167), Expect = 4.090e-11 Identity = 62/237 (26.16%), Postives = 110/237 (46.41%), Query Frame = 2
Query: 3350 YKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFG---NIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGN 4051
+ V LV S PF + ++I++ +++ + I+ + + K+L L L Y F +F E ++KM +++F+ +NIFD V + GSL ++ + S L LR R++R+ KV S+R L+ + + S +++ + L+ L I+ ++GMQ+FG N E Q NF F+ LL +F+ TGE W+ M K+ +
Sbjct: 381 FSVRRLVKSQPFYWAVIILVFFNTVCVAIEHYEQ---------GKWL---------TLFLNYAEYVFLCLFMMEMMIKMYGLSIRVYFQSSFNIFDCVVIAGSLFEIIYGLFVQDSSF----GLSVLRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFNFEEGKPPQ-----NFDTFVKALLTVFQILTGEDWNTAMYSGIKAKKSMAAS 590 HSP 8 Score: 68.1662 bits (165), Expect = 8.432e-11 Identity = 75/296 (25.34%), Postives = 124/296 (41.89%), Query Frame = 2
Query: 260 FEYLVILTIIANCVSLALEVHLPQGDRTFIVTK-------------MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD--------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY--QTNKATD------------SQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
FEY +++ I N + L ++ + + + T + + F +F+ E +LKI+A G KN Y + WNI DFI V IG IT L ++ D LR FRA R++ KL+ +++++L + I++ L + LL+ I++IIG++V+ I K S + + NF +L +F+C T E W +++ NK + S + +YFV I L SF MLNL + V+
Sbjct: 1179 FEYFIMVMIALNTLILMMKYYREEKKASSAATHDREQQAYLNYCNTLIFLNSAFTVMFSIECVLKIMAFG---PKN-YFRDPWNIFDFITV-IGSITDVLVSELQDSSFLSLGFLRLFRAARLI---KLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVFGNI------KLISEYQINRHN-----------------------------NFQTFTNGLLLLFRCATGESWQEIMLACDNNKPCEKDIENTNNFDCGSNFTYIYFVSFIFLCSFLMLNLFVAVI 1431
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Planmine SMEST
Match: SMESG000028279.1 (SMESG000028279.1) HSP 1 Score: 875.544 bits (2261), Expect = 0.000e+0 Identity = 474/794 (59.70%), Postives = 607/794 (76.45%), Query Frame = 2
Query: 2330 IVRKKILPYSSLFIFGPHNFIRRVCHFVANLRFFDIFIMMVIFASSMALAAEDPVIFNSLRNRLLEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFY---------------SKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIKETCKGYFYTFNDQMGIQAKKRIWQRNYFNYDNIVNSMITLFTVTTGEGWPDVLKNSIYATYSNEGPIENYKQEIAXXXXXXXXXXXXXXXXXXXALIIVTFQEQGESDKSDLELDKNETKCIDFAINTKPTSRYMPANKNSVYYKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGFLRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFGNIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNFDYLTRDPSILGPHHLDEYVRLWAEFDPMGTGWLYFEDMYKMMKKLGPPVGFGKNCPYRTACCKLIRMNMILDNQKRVNFNSTLFALIRESLQIKMGSVAEIDNLDQELRINMLKVWPSLNKQNRIDTYLPLNKAS--YPLTVGKIYAGMLIMKNWRNF 4660
I RK +LPYS++FIF P N +RR CHFV NLR+FD+FIM+VI ASS+ALAAEDPV SL+N +LEFFD AFT VF +EMILK+ID+G++LHPGSY RD WNI+D VVI A+ +F +KNLNTIKSLRV+RVLRPLKTINRVPKLKAVF+ ++SS+KNVFNILIV+ILFQFIF VIAVQLF GKFFYCND SK +ETC+GYF+ + + + KKR+WQ + F++DNI+ +M+TLFTVTTGEGWP VLKNS+ +T + GPI +Y+QE+AIFYIVFFIVFPFFFVNIFVALII+TFQEQGE++ DL+LDKN+ +CIDFAIN KP RYMP +K ++ Y+VW LVVS PFEY IM++IAL+++ L++K + + E+ S REQ ++C +L +LN AFT++F+ EC+LK++A+ K +F+DPWNIFDF+TV+GS+TDV +SE++ + +LGFLRLFR RLIK+L +GY+IRILLWTFIQSFKALP+VCLLI +LFFIY IIGMQ+FGNI+L + QIN++NNF+ F GLLLLFRCATGESW IM+ ++ K C T CGS+F+Y YF++FI + +FLMLNLFVAVIMDNFDYLTRD SILGPHHLDEY+R+WAE+DP G ++ +MY+M++ + PPVGFGK CPYR A KLIRMNM +D VNF +TLFALIRESL IKM + +D D ELR + K+WP + + +++ +P + + +TVGKIYAG LI++NWR +
Sbjct: 740 IQRKPVLPYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAEDPVKEKSLKNNILEFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKFFYCNDASKSSRETCRGYFFDYAGRSTPETKKRLWQHHDFHFDNILYAMLTLFTVTTGEGWPTVLKNSMESTNIDNGPIADYRQEMAIFYIVFFIVFPFFFVNIFVALIIITFQEQGENELIDLDLDKNQKQCIDFAINEKPLCRYMPKDKTTIKYRVWRLVVSTPFEYFIMVMIALNTLILMMKYYRE--EKKASSAATHDREQQAYLNYCNTLIFLNSAFTVMFSIECVLKIMAFGPKNYFRDPWNIFDFITVIGSITDVLVSELQD----SSFLSLGFLRLFRAARLIKLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVFGNIKLISEYQINRHNNFQTFTNGLLLLFRCATGESWQEIMLACDNNKPCEKDIENTNNFD-------CGSNFTYIYFVSFIFLCSFLMLNLFVAVIMDNFDYLTRDSSILGPHHLDEYIRVWAEYDPGAIGRIHHSEMYEMLRNMEPPVGFGKKCPYRLAYRKLIRMNMPVDENGTVNFTTTLFALIRESLNIKMSTAEYMDIKDMELRQTICKMWP-VQAKKKLNLIVPPDSELVFHKMTVGKIYAGYLIVENWRAY 1519 HSP 2 Score: 536.954 bits (1382), Expect = 1.111e-158 Identity = 337/625 (53.92%), Postives = 431/625 (68.96%), Query Frame = 2
Query: 311 LEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLF--DATQQACYPQSNT---------SCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATDSQWNVLYFVLLIVLGSFFMLNLVLGVLSGXXXXXXXXXXXXXXXLKLRETQRYENMLDGYMNWMETAEELIRFENKQNAEEKKELMKNRQKTKEWLERKMRQDEYICXXXXXXXXXXXXXXXIEN---------------------WIKYIHEMRPVVKHFIKTQPFYWGVITLVLLNTACVAVEHYGQPKFLDRFLFYAEYVFLSIFMTELFIKIFGFGIRDYFRSKFNVFDFIVVTVSLVEAIFQIINPKQSFGFSAXXXXXXXXXXXXXXYWSSLRNLVXXXXXXXXXXXXXXXXXXXXXXXXXXXGMQLFGGDFNFEQGKPTLNFDTFPNAIITVFVILTGENWNEVMYNGIRSQMKTSNSRDFSMIYSSYFLLIVIFGNYTLLNVFLAIAVD 2089
+E HLP+ D+T + ++ E FLGIF EA+LKI+ALGF K AYL++VWNI+DF+VV GLI+ L + D R RA+RVLRP KLV+G SLQVVL SII+AMAPLLQIGLLVLFA+ IFAIIGLE Y G FH TC+K +S L T+++ P SN+ +C + CK YW+GP +G+ +FDNIGYAMLTVFQCITMEGWTD++Y TN A +++N LYFV LI+LGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLR Q+ E +GYM W++ AEE++ E +E+K ++ R++ ++ + + +++ I + E D +++ W H R V+ +K+QPFYW VI LV NT CVA+EHY Q K+L FL YAEYVFL +FM E+ IK++G IR YF+S FN+FD +V+ SL E + S GF+ +R +RLLR+ K+TRYW+SLRNLV SLL+SMRSIISLL LL LFI I LLGMQLFGG+FNFE+GKP NFDTF A++TVF ILTGE+WN VMYNGI+S+ ++ ++S YF+L+V+FGNYTLLNVFLAIAVD
Sbjct: 1 MERHLPKKDKTLLAGQLELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPS--FDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCFKANSDKLPLPIPTEKSLVPCSNSKNGSGLGAYTCPEDYQCKAYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIMYHTNDAIGNRFNWLYFVPLIILGSFFMLNLVLGVLSGEFAKERERVEKRRAFLKLRRQQQTEKEFNGYMEWIQKAEEVLLNEATTTSEDKMRIIAARRRAEKQKYKLLGKNQSIPDFEYEDTELYSETKSMKGNIFIRSYAGIRRRRKERCTSFWRTEKH-FRFSVRRLVKSQPFYWAVIILVFFNTVCVAIEHYEQGKWLTLFLNYAEYVFLCLFMMEMMIKMYGLSIRVYFQSSFNIFDCVVIAGSLFELLGSHYGNFMSLGFTVMRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFNFEEGKPPQNFDTFVKALLTVFQILTGEDWNTVMYNGIQSK---GGVKERGTVFSIYFVLLVVFGNYTLLNVFLAIAVD 619 HSP 3 Score: 123.635 bits (309), Expect = 9.941e-28 Identity = 83/281 (29.54%), Postives = 142/281 (50.53%), Query Frame = 2
Query: 182 PDRSLFIFSENNIIRRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITV-----------NLQADTVD----LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIF------HNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLYQTNKATD 961
P ++FIFS N +RR C ++ R+F+ +++ I A+ ++LA E P +++ + + F G+FT E ILKII LG I+ +Y + +WNILD VV L+ L+ + +++ R +RVLRP K ++ VP L+ V ++ ++ + I ++ + IFA+I +++++G F + +T + FD ++ P+ T K W F +FDNI YAMLT+F T EGW VL + ++T+
Sbjct: 747 PYSAMFIFSPLNSLRRFCHFVVNLRYFDLFIMIVICASSIALAAED--PVKEKSLKNNILEFFDYAFTGVFTVEMILKIIDLGIILHPGSYCRDLWNILDATVVICALVAFIMGQKPSPTGNKLRGSSAKNLNTIKSLRVLRVLRPLKTINRVPKLKAVFDCVVSSLKNVFNILIVYILFQFIFAVIAVQLFQGKFFYCNDASKSSRETCRGYFFDYAGRST-PE---------TKKRLWQHHDF---HFDNILYAMLTLFTVTTGEGWPTVLKNSMESTN 1012 HSP 4 Score: 82.4185 bits (202), Expect = 3.223e-15 Identity = 78/286 (27.27%), Postives = 139/286 (48.60%), Query Frame = 2
Query: 3350 YKVWCLVVSPPFEYLIMIIIALSSITLVIKSHTKLPERAFQSNTKFLREQSTQNDFCLSLKYLNIAFTIIFTSECILKMIAYQFKLFFKDPWNIFDFVTVLGSLTDVFLSEMKSEESTTEIPNLGF--LRLFRVMRLIKVLMRGYSIRILLWTFIQSFKALPWVCLLIGLLFFIYCIIGMQIFG---NIELNDDTQINQNNNFRNFMMGLLLLFRCATGESWHLIMIDTESGKRCACGNLTTGGICLSPQDKLCGSDFSYPYFITFILVTTFLMLNLFVAVIMDNF 4192
+ V LV S PF + ++I++ +++ + I+ + + K+L L L Y F +F E ++KM +++F+ +NIFD V + GSL ++ S + S LGF +R R++R+ KV S+R L+ + + S +++ + L+ L I+ ++GMQ+FG N E Q NF F+ LL +F+ TGE W+ +M + G + GG+ K G+ FS YF+ ++ + +LN+F+A+ +DN
Sbjct: 381 FSVRRLVKSQPFYWAVIILVFFNTVCVAIEHYEQ---------GKWL---------TLFLNYAEYVFLCLFMMEMMIKMYGLSIRVYFQSSFNIFDCVVIAGSLFELLGSHYGNFMS------LGFTVMRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFNFEEGKPPQ-----NFDTFVKALLTVFQILTGEDWNTVMYN---------GIQSKGGV------KERGTVFSI-YFVLLVVFGNYTLLNVFLAIAVDNL 621 HSP 5 Score: 75.0998 bits (183), Expect = 6.420e-13 Identity = 60/204 (29.41%), Postives = 99/204 (48.53%), Query Frame = 2
Query: 2522 LEFFDLAFTSVFLLEMILKVIDVGLVLHPGSYMRDPWNIMDMIVVIIAVFSFFYSKNLNTIKSLRVIRVLRPLKTINRVPKLKAVFETIISSVKXXXXXXXXXXXXXXXXXXXAVQLFSGKFFYCNDLSKRIK-----ETCKGYFYTFNDQMG-----------IQAKKRIWQRNY--FNYDNIVNSMITLFTVTTGEGWPDVL 3079
LE + F +F +E +LK++ +G H +Y+R+ WNIMD +VV+ + S ++LR +RVLRPLK + L+ V ++II ++ + I ++ + IF +I ++ +SG F Y + K T K N + G Q K NY ++DNI +M+T+F T EGW D++
Sbjct: 17 LELTEKYFLGIFCVEALLKIVALGFCCHKEAYLRNVWNIMDFVVVVTGLISHVLPS--FDQRALRALRVLRPLKLVTGFESLQVVLKSIIRAMAPLLQIGLLVLFAITIFAIIGLEFYSGAFHYTCFKANSDKLPLPIPTEKSLVPCSNSKNGSGLGAYTCPEDYQCKAYWEGPNYGITSFDNIGYAMLTVFQCITMEGWTDIM 218 HSP 6 Score: 70.8626 bits (172), Expect = 1.181e-11 Identity = 77/282 (27.30%), Postives = 135/282 (47.87%), Query Frame = 2
Query: 224 RRSCKGLIEQRWFEYLVILTIIANCVSLALEVHLPQGDRTFIVTKMAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQAD-----TVDLRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY---QTNKATDSQWNV--LYFVLLIVLGSFFMLNLVLGV 1039
R S + L++ + F + VI+ + N V +A+E H QG + A E +FL +F E ++K+ L V Y +S +NI D +V+ L + L + ++ RA+R+LR FK+ SL+ ++ S++ +M ++ + L+ +LIFA++G++++ G F+ ++ PQ NFD A+LTVFQ +T E W V+Y Q+ + V +YFVLL+V G++ +LN+ L +
Sbjct: 380 RFSVRRLVKSQPFYWAVIILVFFNTVCVAIE-HYEQGKWLTLFLNYA--EYVFLCLFMMEMMIKMYGLSIRV----YFQSSFNIFDCVVIAGSLFEL-LGSHYGNFMSLGFTVMRALRLLRIFKVTRYWASLRNLVLSLLNSMRSIISLLFLLFLFILIFALLGMQLFGGEFN--------------FEEGKPPQ-----------------------NFDTFVKALLTVFQILTGEDWNTVMYNGIQSKGGVKERGTVFSIYFVLLVVFGNYTLLNVFLAI 616 HSP 7 Score: 68.1662 bits (165), Expect = 7.647e-11 Identity = 75/296 (25.34%), Postives = 124/296 (41.89%), Query Frame = 2
Query: 260 FEYLVILTIIANCVSLALEVHLPQGDRTFIVTK-------------MAGIEPIFLGIFTTEAILKIIALGFIVGKNAYLKSVWNILDFIVVTIGLITVNLQADTVD--------LRTFRAIRVLRPFKLVSGVPSLQVVLTSIIKAMAPLLQIGLLVLFAVLIFAIIGLEVYRGIFHNTCWKTDSPFLFDATQQACYPQSNTSCNAGFTCKPYWDGPFFGLVNFDNIGYAMLTVFQCITMEGWTDVLY--QTNKATD------------SQWNVLYFVLLIVLGSFFMLNLVLGVL 1042
FEY +++ I N + L ++ + + + T + + F +F+ E +LKI+A G KN Y + WNI DFI V IG IT L ++ D LR FRA R++ KL+ +++++L + I++ L + LL+ I++IIG++V+ I K S + + NF +L +F+C T E W +++ NK + S + +YFV I L SF MLNL + V+
Sbjct: 1106 FEYFIMVMIALNTLILMMKYYREEKKASSAATHDREQQAYLNYCNTLIFLNSAFTVMFSIECVLKIMAFG---PKN-YFRDPWNIFDFITV-IGSITDVLVSELQDSSFLSLGFLRLFRAARLI---KLLRQGYTIRILLWTFIQSFKALPYVCLLIAMLFFIYSIIGMQVFGNI------KLISEYQINRHN-----------------------------NFQTFTNGLLLLFRCATGESWQEIMLACDNNKPCEKDIENTNNFDCGSNFTYIYFVSFIFLCSFLMLNLFVAVI 1358 The following BLAST results are available for this feature:
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Voltage-dependent calcium channel type A subunit alpha-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30015099 ID=SMED30015099|Name=Voltage-dependent calcium channel type A subunit alpha-1|organism=Schmidtea mediterranea sexual|type=transcript|length=5403bpback to top protein sequence of SMED30015099-orf-1 >SMED30015099-orf-1 ID=SMED30015099-orf-1|Name=SMED30015099-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1647bp MGKDTIKYLARQTLSISFVGQKPNPDRSLFIFSENNIIRRSCKGLIEQRWback to top Annotated Terms
The following terms have been associated with this transcript:
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