Family with sequence similarity 183 member A

Overview
NameFamily with sequence similarity 183 member A
Smed IDSMED30015096
Length (bp)513
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Family with sequence similarity 183 member A (SMED30015096) t-SNE clustered cells

Violin plots show distribution of expression levels for Family with sequence similarity 183 member A (SMED30015096) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Family with sequence similarity 183 member A (SMED30015096) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Family with sequence similarity 183 member A (SMED30015096) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015096

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30015096SMESG000024586.1 SmedASXL_001330SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015096SMESG000024586.1 Contig1848newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015096SMESG000024586.1 Contig1848uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015096SMESG000053050.1 Contig1848newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015096SMESG000053050.1 Contig1848uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30015096SMESG000024586.1 dd_Smed_v6_8725_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30015096SMESG000024586.1 dd_Smed_v4_8725_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Human
Match: FAM183A (family with sequence similarity 183 member A [Source:HGNC Symbol;Acc:HGNC:34347])

HSP 1 Score: 66.6254 bits (161), Expect = 5.200e-14
Identity = 44/119 (36.97%), Postives = 62/119 (52.10%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            MA    EK   + VHQ +IL E   KE ++  L+T + +NP  KI+ +  KP  + D   E  D  F   I+ A   P KK+ E  T NQE GW  +PL++ +R D RL   +   +IT
Sbjct:    1 MAGHPKEKVIPDEVHQNQILRELYLKELRTQKLYTQYHVNPLRKIHTVTRKPMSWHDNLEEPADARFLNLIHHAAQGPRKKYPETQTENQEVGWDLEPLINPERHDRRLNHFRVCSDIT 119
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Human
Match: FAM183A (family with sequence similarity 183 member A [Source:HGNC Symbol;Acc:HGNC:34347])

HSP 1 Score: 58.9214 bits (141), Expect = 5.625e-11
Identity = 39/102 (38.24%), Postives = 53/102 (51.96%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDL 363
            MA    EK   + VHQ +IL E   KE ++  L+T + +NP  KI+ +  KP  + D   E  D  F   I+ A   P KK+ E  T NQE GW  +PLV +
Sbjct:    1 MAGHPKEKVIPDEVHQNQILRELYLKELRTQKLYTQYHVNPLRKIHTVTRKPMSWHDNLEEPADARFLNLIHHAAQGPRKKYPETQTENQEVGWDLEPLVSV 102
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Zebrafish
Match: fam183a (family with sequence similarity 183 member A [Source:ZFIN;Acc:ZDB-GENE-111103-1])

HSP 1 Score: 100.138 bits (248), Expect = 3.675e-27
Identity = 49/113 (43.36%), Postives = 74/113 (65.49%), Query Frame = 1 
Query:   82 EKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            EK+ ++ VHQ  I  ET+ KE +   L+T F +NP+ K++ L +KP   +    E  D  F +A++ A++EPTKK++ P T +QE GW+S PL+  DRSD RL FP+++ EIT
Sbjct:    6 EKEPLDIVHQNAIHVETIMKELRHQKLYTEFNINPFKKLHVLTDKPMSSIAHKKEEGDPAFLQAVHNAHLEPTKKYTHPQTESQEIGWLSSPLLVTDRSDRRLNFPRQNSEIT 118
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Zebrafish
Match: fam183a (family with sequence similarity 183 member A [Source:ZFIN;Acc:ZDB-GENE-111103-1])

HSP 1 Score: 99.7525 bits (247), Expect = 6.095e-27
Identity = 49/113 (43.36%), Postives = 74/113 (65.49%), Query Frame = 1 
Query:   82 EKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            EK+ ++ VHQ  I  ET+ KE +   L+T F +NP+ K++ L +KP   +    E  D  F +A++ A++EPTKK++ P T +QE GW+S PL+  DRSD RL FP+++ EIT
Sbjct:   15 EKEPLDIVHQNAIHVETIMKELRHQKLYTEFNINPFKKLHVLTDKPMSSIAHKKEEGDPAFLQAVHNAHLEPTKKYTHPQTESQEIGWLSSPLLVTDRSDRRLNFPRQNSEIT 127
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Zebrafish
Match: fam183a (family with sequence similarity 183 member A [Source:ZFIN;Acc:ZDB-GENE-111103-1])

HSP 1 Score: 97.4413 bits (241), Expect = 5.141e-26
Identity = 49/113 (43.36%), Postives = 73/113 (64.60%), Query Frame = 1 
Query:   82 EKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            EK+ ++ VHQ  I  ET+ KE +   L+T F +NP+ K++ L +KP        E  D  F +A++ A++EPTKK++ P T +QE GW+S PL+  DRSD RL FP+++ EIT
Sbjct:   18 EKEPLDIVHQNAIHVETIMKELRHQKLYTEFNINPFKKLHVLTDKPMSSNAHKKEEGDPAFLQAVHNAHLEPTKKYTHPQTESQEIGWLSSPLLVTDRSDRRLNFPRQNSEIT 130
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Xenopus
Match: large1 (LARGE xylosyl- and glucuronyltransferase 1 [Source:Xenbase;Acc:XB-GENE-6250446])

HSP 1 Score: 100.908 bits (250), Expect = 3.116e-27
Identity = 50/109 (45.87%), Postives = 73/109 (66.97%), Query Frame = 1 
Query:   94 INFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            +N +H+E     T++KEN+   L T FG+NPY K++A+A KP  + D   E  D+ F K I+   +EPTKK++EP TT+QE GWI+ PL+  DR+D RL+F ++  EIT
Sbjct:   15 LNAIHRE-----TIQKENRCQKLVTEFGINPYHKVHAIARKPMSWHDNETETADDHFLKIIHHGALEPTKKYTEPQTTSQEIGWITTPLITSDRTDRRLHFFREKTEIT 118
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Xenopus
Match: akap11 (A-kinase anchoring protein 11 [Source:Xenbase;Acc:XB-GENE-968300])

HSP 1 Score: 100.908 bits (250), Expect = 3.116e-27
Identity = 50/109 (45.87%), Postives = 73/109 (66.97%), Query Frame = 1 
Query:   94 INFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            +N +H+E     T++KEN+   L T FG+NPY K++A+A KP  + D   E  D+ F K I+   +EPTKK++EP TT+QE GWI+ PL+  DR+D RL+F ++  EIT
Sbjct:   15 LNAIHRE-----TIQKENRCQKLVTEFGINPYHKVHAIARKPMSWHDNETETADDHFLKIIHHGALEPTKKYTEPQTTSQEIGWITTPLITSDRTDRRLHFFREKTEIT 118
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Mouse
Match: Fam183b (family with sequence similarity 183, member B [Source:MGI Symbol;Acc:MGI:1922679])

HSP 1 Score: 68.5514 bits (166), Expect = 6.635e-15
Identity = 41/109 (37.61%), Postives = 58/109 (53.21%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDF--SVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRL 384
            MA    + K  + VHQ +IL E   KE ++  L+T + +NP  K++ +  KP  + D     ED  F   I+ A   P KK+SE  T  QE GW   PL++ DR D+RL
Sbjct:    1 MAGRVGQMKNQDEVHQNQILRELFLKELRAQKLYTQYHVNPLRKVHTITRKPMSWHDNLEEPEDAKFLNLIHHAAQGPKKKYSETQTEAQEIGWDPNPLINPDRQDHRL 109
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Mouse
Match: Fam183b (family with sequence similarity 183, member B [Source:MGI Symbol;Acc:MGI:1922679])

HSP 1 Score: 68.5514 bits (166), Expect = 6.871e-15
Identity = 41/109 (37.61%), Postives = 58/109 (53.21%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDF--SVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRL 384
            MA    + K  + VHQ +IL E   KE ++  L+T + +NP  K++ +  KP  + D     ED  F   I+ A   P KK+SE  T  QE GW   PL++ DR D+RL
Sbjct:    3 MAGRVGQMKNQDEVHQNQILRELFLKELRAQKLYTQYHVNPLRKVHTITRKPMSWHDNLEEPEDAKFLNLIHHAAQGPKKKYSETQTEAQEIGWDPNPLINPDRQDHRL 111
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Mouse
Match: Fam183b (family with sequence similarity 183, member B [Source:MGI Symbol;Acc:MGI:1922679])

HSP 1 Score: 53.1434 bits (126), Expect = 3.091e-9
Identity = 36/107 (33.64%), Postives = 49/107 (45.79%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRL 384
            MA    + K  + VHQ +IL E   KE ++  L+T + +NP  K                    F   I+ A   P KK+SE  T  QE GW   PL++ DR D+RL
Sbjct:    3 MAGRVGQMKNQDEVHQNQILRELFLKELRAQKLYTQYHVNPLRKA------------------KFLNLIHHAAQGPKKKYSETQTEAQEIGWDPNPLINPDRQDHRL 91
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Mouse
Match: Fam183b (family with sequence similarity 183, member B [Source:MGI Symbol;Acc:MGI:1922679])

HSP 1 Score: 53.1434 bits (126), Expect = 3.466e-9
Identity = 36/107 (33.64%), Postives = 49/107 (45.79%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRL 384
            MA    + K  + VHQ +IL E   KE ++  L+T + +NP  K                    F   I+ A   P KK+SE  T  QE GW   PL++ DR D+RL
Sbjct:    1 MAGRVGQMKNQDEVHQNQILRELFLKELRAQKLYTQYHVNPLRKA------------------KFLNLIHHAAQGPKKKYSETQTEAQEIGWDPNPLINPDRQDHRL 89
[Close]
BLAST of Family with sequence similarity 183 member A vs. UniProt/SwissProt
Match: sp|Q6ZVS7|F183B_HUMAN (Protein FAM183BP OS=Homo sapiens OX=9606 GN=FAM183BP PE=5 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 1.320e-15
Identity = 45/119 (37.82%), Postives = 64/119 (53.78%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            MA    E+   + VHQ +IL E   KE ++  LHT + +NP  K++ +  KP  + D   E  D  F   I+ A   PTKK+ E  T NQE GW S+ LVD +R D+R+   +   +IT
Sbjct:    1 MAGHPKERVVTDEVHQNQILRELYLKELRTQKLHTQYHVNPLRKVHRITRKPMSWHDNLEEPADARFLNLIHHAAQGPTKKYPEAQTENQEIGWDSEALVDPERRDHRMNHFRVYSDIT 119
[Close]
BLAST of Family with sequence similarity 183 member A vs. UniProt/SwissProt
Match: sp|A8QW39|F183A_BOVIN (Protein FAM183A OS=Bos taurus OX=9913 GN=FAM183A PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 8.956e-15
Identity = 43/109 (39.45%), Postives = 58/109 (53.21%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRL 384
            MA    EK   + VHQ +IL E   KE ++  L+T + +NP  K++ +A KP  + D   E  D  F   I+ A   P KK+ E  T  QE GW S+PLV+  R D RL
Sbjct:    1 MAGHQKEKAIADEVHQNQILRELYLKELRTQKLYTQYHVNPLRKVHTIARKPMSWHDNLEEPADARFLNLIHHAAQGPRKKYPETQTEGQEIGWDSEPLVNPQRDDRRL 109
[Close]
BLAST of Family with sequence similarity 183 member A vs. UniProt/SwissProt
Match: sp|Q5NC57|F183B_MOUSE (Protein FAM183B OS=Mus musculus OX=10090 GN=Fam183b PE=1 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 4.641e-14
Identity = 41/109 (37.61%), Postives = 58/109 (53.21%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDF--SVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRL 384
            MA    + K  + VHQ +IL E   KE ++  L+T + +NP  K++ +  KP  + D     ED  F   I+ A   P KK+SE  T  QE GW   PL++ DR D+RL
Sbjct:    1 MAGRVGQMKNQDEVHQNQILRELFLKELRAQKLYTQYHVNPLRKVHTITRKPMSWHDNLEEPEDAKFLNLIHHAAQGPKKKYSETQTEAQEIGWDPNPLINPDRQDHRL 109
[Close]
BLAST of Family with sequence similarity 183 member A vs. UniProt/SwissProt
Match: sp|A6NL82|F183A_HUMAN (Protein FAM183A OS=Homo sapiens OX=9606 GN=FAM183A PE=3 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 2.497e-13
Identity = 44/119 (36.97%), Postives = 62/119 (52.10%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVE--DETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            MA    EK   + VHQ +IL E   KE ++  L+T + +NP  KI+ +  KP  + D   E  D  F   I+ A   P KK+ E  T NQE GW  +PL++ +R D RL   +   +IT
Sbjct:    1 MAGHPKEKVIPDEVHQNQILRELYLKELRTQKLYTQYHVNPLRKIHTVTRKPMSWHDNLEEPADARFLNLIHHAAQGPRKKYPETQTENQEVGWDLEPLINPERHDRRLNHFRVCSDIT 119
[Close]
BLAST of Family with sequence similarity 183 member A vs. TrEMBL
Match: A0A1S8X3L0 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=X801_02790 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 9.874e-35
Identity = 61/115 (53.04%), Postives = 82/115 (71.30%), Query Frame = 1 
Query:   70 TTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            TTN+  +EINFVHQ +I+ ET++KE +   L+T +G+NP+ K+  LA KPN       ED+ F + +NRA +EPTKK+ EP T NQEYGWIS PL+D+DRSD R Y P  +CE+T
Sbjct:    2 TTNA-SREINFVHQAEIMTETIKKELRHQKLYTQYGINPFKKLCPLASKPNAEQREEEEDKHFLEVLNRAALEPTKKYLEPETENQEYGWISLPLMDMDRSDARFYHPNVACEMT 115
[Close]
BLAST of Family with sequence similarity 183 member A vs. TrEMBL
Match: A0A074Z321 (Uncharacterized protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_15101 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 6.044e-34
Identity = 61/121 (50.41%), Postives = 82/121 (67.77%), Query Frame = 1 
Query:   52 LYNYMATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            L N    T +  +EINFVHQ +I+ ET++KE +   L+T +G+NP+ K+  LA KPN       ED+ F + +NRA +EPTKK+ EP T NQEYGWIS PL+D+DRSD R Y P  +CE+T
Sbjct:   68 LINKRKMTTNASREINFVHQAEIMTETIKKELRHQKLYTQYGINPFKKLCPLASKPNAEQREEEEDKHFLEVLNRAALEPTKKYLEPETENQEYGWISLPLMDMDRSDARFYHPNVACEMT 188
[Close]
BLAST of Family with sequence similarity 183 member A vs. TrEMBL
Match: A0A4E0R563 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_007868 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 1.048e-33
Identity = 56/109 (51.38%), Postives = 78/109 (71.56%), Query Frame = 1 
Query:   88 KEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            K IN+VHQ  I+ ET++KE K   L  N+G+NP+ K+  LA+ PN        D+ F + + RA +EPTK+F+EP T NQEYGWIS+PL+++DRSD R Y+PKKSCE++
Sbjct:    7 KSINYVHQNTIMTETIKKELKHQKLFVNYGINPFKKVCPLADNPNRAQSGDQGDKHFLEVLERAALEPTKRFTEPQTENQEYGWISEPLMEIDRSDMRFYYPKKSCEMS 115
[Close]
BLAST of Family with sequence similarity 183 member A vs. TrEMBL
Match: G4VTE9 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_165790 PE=4 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.212e-33
Identity = 56/115 (48.70%), Postives = 81/115 (70.43%), Query Frame = 1 
Query:   70 TTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            + +S  K +N+VHQ  I+ ET++KE K   + T + +NP+ K+Y LA+KPN   +   ED+ F + I RA++EP KK+ EP T NQEYGWISQPL+++DR+D R Y PK +CE+T
Sbjct:    2 SDSSNNKILNYVHQAVIMNETIKKELKHQKIFTEYSINPFKKMYPLADKPNRIQEADAEDKHFAEVIQRASLEPPKKYIEPQTENQEYGWISQPLMEIDRTDPRFYHPKVACEMT 116
[Close]
BLAST of Family with sequence similarity 183 member A vs. TrEMBL
Match: A0A4S2LZ67 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_003848 PE=4 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.972e-33
Identity = 57/109 (52.29%), Postives = 77/109 (70.64%), Query Frame = 1 
Query:   88 KEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            +EINFVHQ +I+ ET++KE +   L+T +G+NP+ K+  LA KPN       ED+ F + +NRA +EPTKK+ E  T NQEYGWIS PL+D+DRSD R Y P  +CE+T
Sbjct:    7 REINFVHQAEIMTETIKKELRHQKLYTQYGINPFKKLCPLAGKPNAEQREEEEDKHFLEVLNRAALEPTKKYLESETENQEYGWISLPLMDMDRSDARFYHPNVACEMT 115
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004222.1 (pep scaffold:Pmarinus_7.0:GL476676:715278:715976:-1 gene:ENSPMAG00000003861.1 transcript:ENSPMAT00000004222.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 3.484e-12
Identity = 27/53 (50.94%), Postives = 33/53 (62.26%), Query Frame = 1 
Query:  256 FKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            F  A  RA   P +KFS P T  QE GW + PL+D DR+D RL FP++  EIT
Sbjct:    1 FTAAFRRAAQGPREKFSFPQTEAQEVGWNNAPLIDTDRTDRRLNFPRQGSEIT 53
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Nematostella
Match: EDO45114 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTZ1])

HSP 1 Score: 100.908 bits (250), Expect = 5.697e-28
Identity = 50/114 (43.86%), Postives = 73/114 (64.04%), Query Frame = 1 
Query:   79 SEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFS--VEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            +E+ E+NFVHQ  IL ET++KE ++  L+T++ +NP+ K+Y L  KPN   D +   ED+ F K I RA+  P  KF  P T+ Q+ GW ++PL+D    D RL+ P+K  EIT
Sbjct:    7 AERPELNFVHQNAILCETIKKEQRNQQLYTSYSVNPFKKMYTLTGKPNSLHDSADGEEDDHFLKVIKRAHETPVDKFDFPQTSAQDIGWNTEPLIDKTWYDQRLHHPRKHSEIT 120
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Medaka
Match: fam183a (family with sequence similarity 183 member A [Source:NCBI gene;Acc:101156807])

HSP 1 Score: 76.2554 bits (186), Expect = 4.609e-18
Identity = 43/114 (37.72%), Postives = 67/114 (58.77%), Query Frame = 1 
Query:   79 SEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKP--NVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            +EK++ + V Q+ I  E ++KE K L  HT F +NP+ K++ L +KP      +   E+  F K ++RA + P KK+S P T +QE GW S PL+ L   D R +F + S ++T
Sbjct:    2 AEKEKPSVVAQDAIHIERIKKEEKLLKQHTKFHINPFRKLHILPDKPMSRRPPEEVSENSDFIKELHRAYLVPKKKYSTPQTESQEIGWESSPLIPLIHQDKRFHFHRVSTDVT 115
[Close]
BLAST of Family with sequence similarity 183 member A vs. Ensembl Medaka
Match: fam183a (family with sequence similarity 183 member A [Source:NCBI gene;Acc:101156807])

HSP 1 Score: 65.0846 bits (157), Expect = 1.114e-13
Identity = 38/96 (39.58%), Postives = 58/96 (60.42%), Query Frame = 1 
Query:   79 SEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVF--VDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVD 360
            +EK++ + V Q+ I  E ++KE K L  HT F +NP+ K++ L +KP      +   E+  F K ++RA + P KK+S P T +QE GW S PLV+
Sbjct:   18 AEKEKPSVVAQDAIHIERIKKEEKLLKQHTKFHINPFRKLHILPDKPMSRRPPEEVSENSDFIKELHRAYLVPKKKYSTPQTESQEIGWESSPLVN 113
[Close]
BLAST of Family with sequence similarity 183 member A vs. Planmine SMEST
Match: SMESG000024586.1 (SMESG000024586.1)

HSP 1 Score: 243.432 bits (620), Expect = 9.223e-84
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 1 
Query:   64 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT
Sbjct:    1 MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 117
[Close]
BLAST of Family with sequence similarity 183 member A vs. Planmine SMEST
Match: SMESG000052525.1 (SMESG000052525.1)

HSP 1 Score: 103.219 bits (256), Expect = 1.007e-28
Identity = 52/112 (46.43%), Postives = 74/112 (66.07%), Query Frame = 1 
Query:   82 EKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAEKPNVFVDFS-VEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDLDRSDNRLYFPKKSCEIT 414
            +K EIN+VHQ+  + E +++E K   L  +F +N +TK+YAL EKPN  ++F   EDE F K      + P +K+ EP T NQEYGWI++PL+D DR+D+R +F K   EIT
Sbjct:    6 DKVEINYVHQDLFVYEKIKQELKYQKLVEHFSINSHTKVYALTEKPNKCINFDEQEDERFIKLFKWNELSPQEKYCEPQTVNQEYGWINEPLMDRDRTDDRFFFLKPQTEIT 117
The following BLAST results are available for this feature:
BLAST of Family with sequence similarity 183 member A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
FAM183A5.200e-1436.97family with sequence similarity 183 member A [Sour... [more]
FAM183A5.625e-1138.24family with sequence similarity 183 member A [Sour... [more]
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
fam183a3.675e-2743.36family with sequence similarity 183 member A [Sour... [more]
fam183a6.095e-2743.36family with sequence similarity 183 member A [Sour... [more]
fam183a5.141e-2643.36family with sequence similarity 183 member A [Sour... [more]
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
large13.116e-2745.87LARGE xylosyl- and glucuronyltransferase 1 [Source... [more]
akap113.116e-2745.87A-kinase anchoring protein 11 [Source:Xenbase;Acc:... [more]
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Fam183b6.635e-1537.61family with sequence similarity 183, member B [Sou... [more]
Fam183b6.871e-1537.61family with sequence similarity 183, member B [Sou... [more]
Fam183b3.091e-933.64family with sequence similarity 183, member B [Sou... [more]
Fam183b3.466e-933.64family with sequence similarity 183, member B [Sou... [more]
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BLAST of Family with sequence similarity 183 member A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6ZVS7|F183B_HUMAN1.320e-1537.82Protein FAM183BP OS=Homo sapiens OX=9606 GN=FAM183... [more]
sp|A8QW39|F183A_BOVIN8.956e-1539.45Protein FAM183A OS=Bos taurus OX=9913 GN=FAM183A P... [more]
sp|Q5NC57|F183B_MOUSE4.641e-1437.61Protein FAM183B OS=Mus musculus OX=10090 GN=Fam183... [more]
sp|A6NL82|F183A_HUMAN2.497e-1336.97Protein FAM183A OS=Homo sapiens OX=9606 GN=FAM183A... [more]
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BLAST of Family with sequence similarity 183 member A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S8X3L09.874e-3553.04Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A074Z3216.044e-3450.41Uncharacterized protein (Fragment) OS=Opisthorchis... [more]
A0A4E0R5631.048e-3351.38Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
G4VTE99.212e-3348.70Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A4S2LZ679.972e-3352.29Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000004222.13.484e-1250.94pep scaffold:Pmarinus_7.0:GL476676:715278:715976:-... [more]
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO451145.697e-2843.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Family with sequence similarity 183 member A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
fam183a4.609e-1837.72family with sequence similarity 183 member A [Sour... [more]
fam183a1.114e-1339.58family with sequence similarity 183 member A [Sour... [more]
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BLAST of Family with sequence similarity 183 member A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000024586.19.223e-84100.00SMESG000024586.1[more]
SMESG000052525.11.007e-2846.43SMESG000052525.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015096 ID=SMED30015096|Name=Family with sequence similarity 183 member A|organism=Schmidtea mediterranea sexual|type=transcript|length=513bp
TAAAAATATTGCTGAAATTTAAATTTATTTATTATTAAATATTTTATTAA
TCTCTATAATTATATGGCTACAACAAATTCTGAGAAAAAAGAAATTAATT
TTGTCCACCAAGAAAAGATTTTAGCTGAAACTGTTCGCAAGGAAAATAAA
TCTTTAGTTTTGCATACGAATTTTGGCCTAAATCCCTACACAAAAATTTA
CGCGCTGGCGGAGAAGCCAAATGTTTTCGTCGATTTTTCAGTAGAAGATG
AAACATTCAAAAAAGCGATCAACAGGGCCAATATTGAACCGACCAAAAAA
TTCTCAGAGCCCATAACGACAAACCAAGAGTACGGGTGGATAAGTCAGCC
ATTGGTTGACCTTGACAGGAGTGACAATCGACTCTATTTCCCGAAAAAGA
GTTGTGAAATAACAAAACTGATGAAATATGAAAAATCAGAAAAATCATCA
AAGCCAGAAGCCACAAAATCATAGAATAATTTTTATAATAAAATTTTTTT
CGACTTAAAATAA
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protein sequence of SMED30015096-orf-1

>SMED30015096-orf-1 ID=SMED30015096-orf-1|Name=SMED30015096-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=137bp
MATTNSEKKEINFVHQEKILAETVRKENKSLVLHTNFGLNPYTKIYALAE
KPNVFVDFSVEDETFKKAINRANIEPTKKFSEPITTNQEYGWISQPLVDL
DRSDNRLYFPKKSCEITKLMKYEKSEKSSKPEATKS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR029214FAM183
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029214FAM183 familyPFAMPF14886FAM183coord: 18..118
e-value: 2.7E-25
score: 89.2
IPR029214FAM183 familyPANTHERPTHR33865FAMILY NOT NAMEDcoord: 2..135
NoneNo IPR availablePANTHERPTHR33865:SF3PROTEIN FAM183Bcoord: 2..135