Spinster-like protein 1

Overview
NameSpinster-like protein 1
Smed IDSMED30015055
Length (bp)1594
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Spinster-like protein 1 (SMED30015055) t-SNE clustered cells

Violin plots show distribution of expression levels for Spinster-like protein 1 (SMED30015055) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Spinster-like protein 1 (SMED30015055) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Spinster-like protein 1 (SMED30015055) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30015055

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30015055h1SMcG0017548 dd_Smed_v4_4119_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Spinster-like protein 1 vs. Ensembl Human
Match: SPNS1 (sphingolipid transporter 1 (putative) [Source:HGNC Symbol;Acc:HGNC:30621])

HSP 1 Score: 362.844 bits (930), Expect = 2.795e-119
Identity = 200/455 (43.96%), Postives = 288/455 (63.30%), Query Frame = 2
Query:  194 KDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            +  ++V V+  INLLNYMDR+T+ G+LPDI+ ++NI D  SGL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP + FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GVV + +LF +V EP RG+ +    +    PTSW  D++ L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLR   P ADPLVCA GL+  AP   LS+  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I   L   +     S  S + ++Q+++++ A +  +GG  F+  + +I  D+ +   +V        S +D
Sbjct:   57 RSALIVAVLCYINLLNYMDRFTVAGVLPDIEQFFNIGDSSSGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVERHSDLPPLNPTSWWADLRALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCATGLLGSAPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRNW---PPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRRRAQLHVQGLLHEAGSTDD 508          
BLAST of Spinster-like protein 1 vs. Ensembl Human
Match: SPNS1 (sphingolipid transporter 1 (putative) [Source:HGNC Symbol;Acc:HGNC:30621])

HSP 1 Score: 363.229 bits (931), Expect = 9.775e-119
Identity = 200/455 (43.96%), Postives = 288/455 (63.30%), Query Frame = 2
Query:  194 KDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            +  ++V V+  INLLNYMDR+T+ G+LPDI+ ++NI D  SGL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP + FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GVV + +LF +V EP RG+ +    +    PTSW  D++ L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLR   P ADPLVCA GL+  AP   LS+  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I   L   +     S  S + ++Q+++++ A +  +GG  F+  + +I  D+ +   +V        S +D
Sbjct:  102 RSALIVAVLCYINLLNYMDRFTVAGVLPDIEQFFNIGDSSSGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVERHSDLPPLNPTSWWADLRALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCATGLLGSAPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRNW---PPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRRRAQLHVQGLLHEAGSTDD 553          
BLAST of Spinster-like protein 1 vs. Ensembl Human
Match: SPNS1 (sphingolipid transporter 1 (putative) [Source:HGNC Symbol;Acc:HGNC:30621])

HSP 1 Score: 348.206 bits (892), Expect = 1.871e-114
Identity = 192/438 (43.84%), Postives = 276/438 (63.01%), Query Frame = 2
Query:  245 MDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            MDR+T+ G+LPDI+ ++NI D  SGL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP + FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GVV + +LF +V EP RG+ +    +    PTSW  D++ L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLR   P ADPLVCA GL+  AP   LS+  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I   L   +     S  S + ++Q+++++ A +  +GG  F+  + +I  D+ +   +V        S +D
Sbjct:    1 MDRFTVAGVLPDIEQFFNIGDSSSGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVERHSDLPPLNPTSWWADLRALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCATGLLGSAPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRNW---PPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRRRAQLHVQGLLHEAGSTDD 435          
BLAST of Spinster-like protein 1 vs. Ensembl Human
Match: SPNS1 (sphingolipid transporter 1 (putative) [Source:HGNC Symbol;Acc:HGNC:30621])

HSP 1 Score: 337.806 bits (865), Expect = 2.420e-109
Identity = 186/389 (47.81%), Postives = 256/389 (65.81%), Query Frame = 2
Query:  194 KDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALR-----FTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGI 1324
            +  ++V V+  INLLNYMDR+T+ G+LPDI+ ++NI D  SGL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP + FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR      TP +GVV + +LF +V EP RG+ +    +    PTSW  D++ L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLR   P ADPLVCA GL+  AP   LS+  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+
Sbjct:   46 RSALIVAVLCYINLLNYMDRFTVAGVLPDIEQFFNIGDSSSGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDMAGDWHWALRPSVSQVTPGLGVVAVLLLFLVVREPPRGAVERHSDLPPLNPTSWWADLRALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCATGLLGSAPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGL 434          
BLAST of Spinster-like protein 1 vs. Ensembl Human
Match: SPNS2 (sphingolipid transporter 2 [Source:HGNC Symbol;Acc:HGNC:26992])

HSP 1 Score: 334.724 bits (857), Expect = 3.973e-108
Identity = 182/432 (42.13%), Postives = 262/432 (60.65%), Query Frame = 2
Query:  230 NLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD--GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADS---NYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENV 1501
            N+LNY+DRYT+ G+L DI+ ++ + D  +GLLQ+ FI S+MV   +FGYLGDR NRK+I+ CG+ FWS +T +SSFIP+  FWL V+ R L+G+GEASYST+AP II DLFTK  RT +++ F FAIP+GSGLG+I G ++K   G W  ALR +P++G++   ++  +V    RG AD  G +    TSW+ D+K L+ N S++F +L  + V F  G+L  +  +Y+  A   +K              + IFG++T   G  GVV GA A R  R     ADPLVCA G++  A    L  V AK     + + IF+ +TLL  NWA+  D+ M VV P RR +A A Q    H LGDAGSPY +G I    +D  R   S+ DS    + S+ YA+++   ++V+GG FF+  + +  +D+ +  + V
Sbjct:  112 NVLNYLDRYTVAGVLLDIQQHFGVKDRGAGLLQSVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILILVPATKRGHADQLGDQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTAETCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLKTQRADPLVCAVGMLGSAIFICLIFVAAKSSIVGAYICIFVGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFI----SDLIR--QSTKDSPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFVSDRARAEQQV 537          
BLAST of Spinster-like protein 1 vs. Ensembl Celegans
Match: spin-1 (SPINster (Drosophila lysosomal permease) homolog [Source:UniProtKB/TrEMBL;Acc:O01927])

HSP 1 Score: 331.643 bits (849), Expect = 1.395e-107
Identity = 174/463 (37.58%), Postives = 282/463 (60.91%), Query Frame = 2
Query:  203 IVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI-----PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKA-YNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRR-IFPY-------ADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARIL 1549
            +VV ++FIINLLNYMDRYTI G+L D++ YYNI D  +GL+QT+F++ +++   + G+LGDR NRK I + G+  W     AS+FIP + FWLF++ R ++G+GEASY+ ++P +I+D+FT   R++++  F FAIP G GLGF++G A+ +W G WQ  +R T ++G+VCL ++   V EP RG A+  K  I      TS+++D+K LL N +++  +LG+T   F++G+L+W+A I + YA+ A  N  +      NIN +FG++T + G+ GV IG        R + P+       AD LVCA G  +  P  +L++   + + N++  ++FI      FNWA   D+ +SVV P RR SA+++Q+++ H  GDA  PY +G+I    +D  R +  +A ++Y S+  +  +    LV+    F  ++  +  DK + +E  I  +++N  N  +  L
Sbjct:   57 VVVAILFIINLLNYMDRYTIAGVLNDVQTYYNISDAWAGLIQTTFMVFFIIFSPICGFLGDRYNRKWIFVVGIAIWVSAVFASTFIPSNQFWLFLLFRGIVGIGEASYAIISPTVIADMFTGVLRSRMLMVFYFAIPFGCGLGFVVGSAVASWTGHWQWGVRVTGVLGIVCLLLIIVFVREPERGKAEREKGEIAASTEATSYLDDMKDLLSNATYVTSSLGYTATVFMVGTLAWWAPITIQYADSARRNGTITEDQKANINLVFGALTCVGGVLGVAIGTLVSNMWSRGVGPFKHIQTVRADALVCAIGAAICIPTLILAIQNIESNMNFAWGMLFICIVASSFNWATNVDLLLSVVVPQRRSSASSWQILISHMFGDASGPYILGLI----SDAIRGNEDTAQAHYKSLVTSFWLCVGTLVLSVILFGISAITVVKDKARFNE--IMLAQANKDNTSSGTL 513          
BLAST of Spinster-like protein 1 vs. Ensembl Celegans
Match: spin-4 (SPINster (Drosophila lysosomal permease) homolog [Source:UniProtKB/TrEMBL;Acc:A0A3B1DQ22])

HSP 1 Score: 314.694 bits (805), Expect = 3.008e-101
Identity = 169/455 (37.14%), Postives = 273/455 (60.00%), Query Frame = 2
Query:  152 SNGKSFRAAFKLQ-KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKS-IPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQY--LDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLR---RIF-----PYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDK 1480
              G     A +L  KK ++   ++ ++NLLNYMDRYTI G++  +  +++IDD+  GLLQT FI+ YM    LFGYLGDR NRK++++ G+  W L   ASSF  +  ++LF++ R ++G+GEASYST+AP ++SDLF+   R++++  F FAIP+GSGLGFI G ++      WQ  +RF+PIIG+ CL ++ ++++EPVRG+ DG +++      I DIK L+   +F   +       F +G++SW+   Y+G++    +    +  +    IN IFG +T +AGL GV  G+   R  R    IF       AD  +CA  + +  P    ++ +A+Y  N  L++I+ A   +C NW++  D+ M VV   RR +A A Q +V H  GDA SPY +G+    L+D  R   +SA  +++++Q A+ +   +LV+ G F++ A+F++ +D+
Sbjct:    6 EEGSGLDVASRLAGKKKWVTCSILLLVNLLNYMDRYTIVGVMSRLATFFDIDDKGQGLLQTVFIVFYMFFAPLFGYLGDRYNRKMLMITGICIWILAVFASSFCGEGHYYLFLLCRGIVGIGEASYSTIAPTVLSDLFSGGLRSRVLMMFYFAIPVGSGLGFISGSSISQATDSWQWGVRFSPIIGIACLGLMLWLLDEPVRGACDGARQNGDEADLIGDIKYLMSIKTFYLASAASIASFFSIGTMSWWTPQYVGFSYAVIHNVPKVPETELTQINLIFGIITCMAGLLGVATGSILSRAWRDGSSIFRNHATEKADVYICALSMFVALPFLFFAIFIAEYSTNGCLILIYFAIMSMCLNWSVNVDVLMYVVVANRRATALAVQTMVAHLFGDAASPYIIGV----LSDMLRGDDASAVGHFFALQKALYVPTFMLVVAGAFYLAATFFVEDDR 456          
BLAST of Spinster-like protein 1 vs. Ensembl Celegans
Match: spin-4 (SPINster (Drosophila lysosomal permease) homolog [Source:UniProtKB/TrEMBL;Acc:A0A3B1DQ22])

HSP 1 Score: 314.694 bits (805), Expect = 3.008e-101
Identity = 169/455 (37.14%), Postives = 273/455 (60.00%), Query Frame = 2
Query:  152 SNGKSFRAAFKLQ-KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKS-IPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQY--LDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLR---RIF-----PYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDK 1480
              G     A +L  KK ++   ++ ++NLLNYMDRYTI G++  +  +++IDD+  GLLQT FI+ YM    LFGYLGDR NRK++++ G+  W L   ASSF  +  ++LF++ R ++G+GEASYST+AP ++SDLF+   R++++  F FAIP+GSGLGFI G ++      WQ  +RF+PIIG+ CL ++ ++++EPVRG+ DG +++      I DIK L+   +F   +       F +G++SW+   Y+G++    +    +  +    IN IFG +T +AGL GV  G+   R  R    IF       AD  +CA  + +  P    ++ +A+Y  N  L++I+ A   +C NW++  D+ M VV   RR +A A Q +V H  GDA SPY +G+    L+D  R   +SA  +++++Q A+ +   +LV+ G F++ A+F++ +D+
Sbjct:    6 EEGSGLDVASRLAGKKKWVTCSILLLVNLLNYMDRYTIVGVMSRLATFFDIDDKGQGLLQTVFIVFYMFFAPLFGYLGDRYNRKMLMITGICIWILAVFASSFCGEGHYYLFLLCRGIVGIGEASYSTIAPTVLSDLFSGGLRSRVLMMFYFAIPVGSGLGFISGSSISQATDSWQWGVRFSPIIGIACLGLMLWLLDEPVRGACDGARQNGDEADLIGDIKYLMSIKTFYLASAASIASFFSIGTMSWWTPQYVGFSYAVIHNVPKVPETELTQINLIFGIITCMAGLLGVATGSILSRAWRDGSSIFRNHATEKADVYICALSMFVALPFLFFAIFIAEYSTNGCLILIYFAIMSMCLNWSVNVDVLMYVVVANRRATALAVQTMVAHLFGDAASPYIIGV----LSDMLRGDDASAVGHFFALQKALYVPTFMLVVAGAFYLAATFFVEDDR 456          
BLAST of Spinster-like protein 1 vs. Ensembl Celegans
Match: spin-2 (SPINster (Drosophila lysosomal permease) homolog [Source:UniProtKB/TrEMBL;Acc:Q18545])

HSP 1 Score: 304.679 bits (779), Expect = 1.309e-97
Identity = 169/454 (37.22%), Postives = 263/454 (57.93%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSI----PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSM------NINYIFGSMTVLAGLFGVVIGAEAGRRL-------RRI-FPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFK 1486
             ++D+I V  +F++NLLNY+DRYT+ G+L  ++ YYNI D   GL+QT F++S+MV   + GYLGDR NRK I++ GV  W    L SSF+P + FWLF+V+RS +G+GEASYS VAP++ISD+F  + R+ +   F FAIP+GSGLGFI+G  + T  G WQ  +R + I G++ +  L     EP RG+AD   G  K +     T+++ED+  LL  P+ +  T G+T + FV G+LSW+    + +   A++Q L+++  +       +   FG++T   GL GV+ G+   + L       RR+    A PLV   G +L AP  L+ M+          ++IF   T +CFNW L  DM  +V++P RR +A ++ ++V H  GDA  PY +G+I    +D  R  ++     Y+S+  A      +L++    +  +S  + +D+ K
Sbjct:    4 SQQDYISVTALFVVNLLNYVDRYTVAGVLTQVQTYYNISDSLGGLIQTVFLISFMVFSPVCGYLGDRFNRKWIMIIGVGIWLGAVLGSSFVPANHFWLFLVLRSFVGIGEASYSNVAPSLISDMFNGQKRSTVFMIFYFAIPVGSGLGFIVGSNVATLTGHWQWGIRVSAIAGLIVMIALVLFTYEPERGAADKAMGESKDVVVTTNTTYLEDLVILLKTPTLVACTWGYTALVFVSGTLSWWEPTVIQHL-TAWHQGLNDTKDLASTDKDRVALYFGAITTAGGLIGVIFGSMLSKWLVAGWGPFRRLQTDRAQPLVAGGGALLAAPFLLIGMIFGDKSLVLLYIMIFFGITFMCFNWGLNIDMLTTVIHPNRRSTAFSYFVLVSHLFGDASGPYLIGLI----SDAIRHGSTYPKDQYHSLVSATYCCVALLLLSAGLYFVSSLTLVSDRKK 452          
BLAST of Spinster-like protein 1 vs. Ensembl Celegans
Match: spin-3 (SPINster (Drosophila lysosomal permease) homolog [Source:UniProtKB/TrEMBL;Acc:Q19235])

HSP 1 Score: 282.337 bits (721), Expect = 4.234e-89
Identity = 155/476 (32.56%), Postives = 264/476 (55.46%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKS-----IPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQY-----LDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPY-----------ADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILT 1552
            ++KD+I + ++F++NL+N +DRYTI G+LPD++ YYNI+D   G++QT F++S+M+   + GYLGDR NRK +++ G+  W +   AS+FIP+++F LF+  RSL+G+GEASY  + P +ISD+FT + RT++   F  A+P+GSGLG+II   + +  G WQ  +R T I G+V L  L F+V+EP RG+A+ ++         TS+ +D+K LL  P+++  TL +T + FV G+L+W+    + Y+      Y     L  S       IFG +T   G+ G ++    G  L + F Y           A  +    G ++  PC ++  V        + +++ ++ T LCFNW+L  ++   +V P RR +A ++  ++ H  GDA  PY +G I  ++         S + +Y S+ YA ++   ++ I    +  A+     D    +  +++    N S++  +  T
Sbjct:    4 ERKDYISIVILFVVNLINNVDRYTIAGVLPDVQSYYNINDSMGGMIQTVFLISFMIGSPICGYLGDRFNRKYVMLVGIVIWLICVCASTFIPRNLFPLFLFFRSLVGIGEASYVNICPTMISDMFTSDKRTRVYMLFYLAVPVGSGLGYIISSNVSSLTGSWQWGVRVTGIGGIVALLALLFMVHEPERGAAEKLEGKDTTVRPSTSYWKDLKVLLKCPTYVVTTLAYTALIFVSGTLTWWMPTIIEYSAAWTRGYKSIKELPLSFKNETGLIFGLLTTACGIIGTLL----GNLLAQCFLYGWLGAWSKTKRAHLVAAGCGALISTPCLVVVFVFGHSSELLTWIMVGVSITGLCFNWSLNVEVFNQIVAPERRSTAFSYVTLISHLCGDASGPYIIGAISDAIKSNH---LDSPEWDYKSLAYASMLAPVMMGISTVLYFVAAIIFKRD----ARRLVREMTRNESDDQEKSYT 468          
BLAST of Spinster-like protein 1 vs. Ensembl Fly
Match: spin (gene:FBgn0086676 transcript:FBtr0089593)

HSP 1 Score: 346.28 bits (887), Expect = 4.112e-112
Identity = 194/498 (38.96%), Postives = 290/498 (58.23%), Query Frame = 2
Query:  191 KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF--AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIK------------------------------------------SSLNDKFRISASSADSN---YYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDAR 1543
            +  +  V V+  +NL+NYMDR+TI G+L D++  ++I ++ +GLLQT F++SYMVC  +FGYLGDR +R  I+  GV  WS  TL  SF+ +  F  F+  R+L+G+GEASYST+AP IISDLF  + R+K++A F FAIP+GSGLG+I+G         W+ ALR TPI+G+V +F++  I  +PVRG ++G      T++ +DIK L+ N SFM  T GFT V FV G+L+W+  + IY+G   +  N+   N    ++ + FG +T+LAGL GV +G+   + L + +P ADP++CAFGL++ AP    + +L   +   +  +IF  Q  L  NWA+V D+ + VV P RR +A AFQ+++ HALGDAGSPY VG I                                           +SL +  R SAS   S+   +  +QYA+   + + V+GG FFIF + +I  DK+  +   ++ + +     D R
Sbjct:  111 RSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQ--FGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLI-KDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNE---NIVQDDVAFNFGVITMLAGLLGVPLGSFLSQYLVKRYPTADPVICAFGLLVSAPLLTGACLLVNSNSVGTYALIFFGQLALNLNWAIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRG-LQDATAQQQQRDER 601          
BLAST of Spinster-like protein 1 vs. Ensembl Fly
Match: spin (gene:FBgn0086676 transcript:FBtr0089590)

HSP 1 Score: 343.584 bits (880), Expect = 7.755e-111
Identity = 192/484 (39.67%), Postives = 285/484 (58.88%), Query Frame = 2
Query:  191 KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF--AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIK------------------------------------------SSLNDKFRISASSADSN---YYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENV 1501
            +  +  V V+  +NL+NYMDR+TI G+L D++  ++I ++ +GLLQT F++SYMVC  +FGYLGDR +R  I+  GV  WS  TL  SF+ +  F  F+  R+L+G+GEASYST+AP IISDLF  + R+K++A F FAIP+GSGLG+I+G         W+ ALR TPI+G+V +F++  I  +PVRG ++G      T++ +DIK L+ N SFM  T GFT V FV G+L+W+  + IY+G   +  N+   N    ++ + FG +T+LAGL GV +G+   + L + +P ADP++CAFGL++ AP    + +L   +   +  +IF  Q  L  NWA+V D+ + VV P RR +A AFQ+++ HALGDAGSPY VG I                                           +SL +  R SAS   S+   +  +QYA+   + + V+GG FFIF + +I  DK+  +  +
Sbjct:  111 RSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQ--FGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLI-KDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNE---NIVQDDVAFNFGVITMLAGLLGVPLGSFLSQYLVKRYPTADPVICAFGLLVSAPLLTGACLLVNSNSVGTYALIFFGQLALNLNWAIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRGL 588          
BLAST of Spinster-like protein 1 vs. Ensembl Fly
Match: spin (gene:FBgn0086676 transcript:FBtr0089591)

HSP 1 Score: 342.043 bits (876), Expect = 1.734e-110
Identity = 192/498 (38.55%), Postives = 287/498 (57.63%), Query Frame = 2
Query:  191 KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF--AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIK------------------------------------------SSLNDKFRISASSADSN---YYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDAR 1543
            +  +  V V+  +NL+NYMDR+TI G+L D++  ++I ++ +GLLQT F++SYMVC  +FGYLGDR +R  I+  GV  WS  TL  SF+ +  F  F+  R+L+G+GEASYST+AP IISDLF  + R+K++A F FAIP+GSGLG+I+G         W+ ALR TPI+G+V +F++  I  +PVRG ++G      T++ +DIK L+ N SFM  T GFT V FV G+L+W+  + IY+G   +  N+   N    +I+Y FG + +LAGL GV +G+   +RLR  +   DP +CA GL + AP    ++V+ +   +     +F+AQ  L   W++V D+ + VV P RR +A AFQ+++ HALGDAGSPY VG I                                           +SL +  R SAS   S+   +  +QYA+   + + V+GG FFIF + +I  DK+  +   ++ + +     D R
Sbjct:  111 RSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQ--FGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLI-KDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNE---NIVQDDISYKFGLVAMLAGLIGVPLGSFLAQRLRGRYENCDPYICAVGLFISAPMVFAALVVPQTSESLCFFFVFVAQVALNLCWSIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRG-LQDATAQQQQRDER 601          
BLAST of Spinster-like protein 1 vs. Ensembl Fly
Match: spin (gene:FBgn0086676 transcript:FBtr0089592)

HSP 1 Score: 339.732 bits (870), Expect = 2.652e-109
Identity = 190/484 (39.26%), Postives = 282/484 (58.26%), Query Frame = 2
Query:  191 KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF--AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIK------------------------------------------SSLNDKFRISASSADSN---YYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENV 1501
            +  +  V V+  +NL+NYMDR+TI G+L D++  ++I ++ +GLLQT F++SYMVC  +FGYLGDR +R  I+  GV  WS  TL  SF+ +  F  F+  R+L+G+GEASYST+AP IISDLF  + R+K++A F FAIP+GSGLG+I+G         W+ ALR TPI+G+V +F++  I  +PVRG ++G      T++ +DIK L+ N SFM  T GFT V FV G+L+W+  + IY+G   +  N+   N    +I+Y FG + +LAGL GV +G+   +RLR  +   DP +CA GL + AP    ++V+ +   +     +F+AQ  L   W++V D+ + VV P RR +A AFQ+++ HALGDAGSPY VG I                                           +SL +  R SAS   S+   +  +QYA+   + + V+GG FFIF + +I  DK+  +  +
Sbjct:  111 RSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFMKQ--FGWFIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVFLILLI-KDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNE---NIVQDDISYKFGLVAMLAGLIGVPLGSFLAQRLRGRYENCDPYICAVGLFISAPMVFAALVVPQTSESLCFFFVFVAQVALNLCWSIVADILLYVVVPTRRSTAEAFQILISHALGDAGSPYLVGAISEAIMKHLHKNPSDSGLTTELRSMSQVAGSAISNATQVIAEATTSLMETARSSASQEYSDVEQFEGLQYALFSTSFVEVLGGIFFIFTACFIIKDKYNATRGL 588          
BLAST of Spinster-like protein 1 vs. Ensembl Fly
Match: spin (gene:FBgn0086676 transcript:FBtr0089589)

HSP 1 Score: 233.032 bits (593), Expect = 5.371e-71
Identity = 118/251 (47.01%), Postives = 170/251 (67.73%), Query Frame = 2
Query:  191 KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF--AVIYMGYAEKAYNQ 937
            +  +  V V+  +NL+NYMDR+TI G+L D++  ++I ++ +GLLQT F++SYMVC  +FGYLGDR +R  I+  GV  WS  TL  SF+ K   W F+  R+L+G+GEASYST+AP IISDLF  + R+K++A F FAIP+GSGLG+I+G         W+ ALR TPI+G+V +F L  ++ +PVRG ++G      T++ +DIK L+ N SFM  T GFT V FV G+L+W+  + IY+G   +  N+
Sbjct:  111 RSQWFTVTVLCFVNLINYMDRFTIAGVLTDVRNDFDIGNDSAGLLQTVFVISYMVCAPIFGYLGDRYSRPWIMAVGVGLWSTTTLLGSFM-KQFGW-FIAFRALVGIGEASYSTIAPTIISDLFVHDMRSKMLALFYFAIPVGSGLGYIVGSKTAHLANDWRWALRVTPILGIVAVF-LILLIKDPVRGHSEGSHNLEATTYKQDIKALVRNRSFMLSTAGFTCVAFVAGALAWWGPSFIYLGMKMQPGNE 358          
BLAST of Spinster-like protein 1 vs. Ensembl Zebrafish
Match: spns1 (spinster homolog 1 (Drosophila) [Source:NCBI gene;Acc:799873])

HSP 1 Score: 380.563 bits (976), Expect = 1.040e-126
Identity = 207/468 (44.23%), Postives = 296/468 (63.25%), Query Frame = 2
Query:  131 LSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAV-------IYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIK 1507
            L E + S +G + R A        + V V+  INLLNYMDR+T+ G+LPDI+ ++ I D  SGLLQT FI SYM    LFGYLGDR NRKLI+  G+ FWS++TLASSFI KD FW  ++ R L+GVGEASYST+AP II+DLF KE RT +++ F FAIP+GSG+G+I+G  + T    W  ALR TP +G++ +F+L  +V EP RG+ +   +     TSW+ D+K L  NPSF+  T GFT V FV GSL+ +A        ++ G  +  +    D+S S+    IFG++TV+ G+ GV  G +A + LR   P ADPLVCA GL+L AP   LS++ A+     + V IF+ +T L  NWA+V D+ + VV P RR +A AFQ+++ H LGDA SPY +G++  S+ +     ++S    + S+Q ++L+ + + V GG FF+  + +I  D+  ++EN + 
Sbjct:   30 LPEDEESPSGVTDRRAI-------MTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSL----IFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKE-----SNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDR-DLAENYVP 480          
BLAST of Spinster-like protein 1 vs. Ensembl Zebrafish
Match: spns1 (spinster homolog 1 (Drosophila) [Source:NCBI gene;Acc:799873])

HSP 1 Score: 380.563 bits (976), Expect = 1.040e-126
Identity = 207/468 (44.23%), Postives = 296/468 (63.25%), Query Frame = 2
Query:  131 LSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAV-------IYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIK 1507
            L E + S +G + R A        + V V+  INLLNYMDR+T+ G+LPDI+ ++ I D  SGLLQT FI SYM    LFGYLGDR NRKLI+  G+ FWS++TLASSFI KD FW  ++ R L+GVGEASYST+AP II+DLF KE RT +++ F FAIP+GSG+G+I+G  + T    W  ALR TP +G++ +F+L  +V EP RG+ +   +     TSW+ D+K L  NPSF+  T GFT V FV GSL+ +A        ++ G  +  +    D+S S+    IFG++TV+ G+ GV  G +A + LR   P ADPLVCA GL+L AP   LS++ A+     + V IF+ +T L  NWA+V D+ + VV P RR +A AFQ+++ H LGDA SPY +G++  S+ +     ++S    + S+Q ++L+ + + V GG FF+  + +I  D+  ++EN + 
Sbjct:   30 LPEDEESPSGVTDRRAI-------MTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSL----IFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKE-----SNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDR-DLAENYVP 480          
BLAST of Spinster-like protein 1 vs. Ensembl Zebrafish
Match: spns1 (spinster homolog 1 (Drosophila) [Source:NCBI gene;Acc:799873])

HSP 1 Score: 379.407 bits (973), Expect = 3.194e-126
Identity = 201/448 (44.87%), Postives = 289/448 (64.51%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAV-------IYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVI 1504
             ++  + V V+  INLLNYMDR+T+ G+LPDI+ ++ I D  SGLLQT FI SYM    LFGYLGDR NRKLI+  G+ FWS++TLASSFI KD FW  ++ R L+GVGEASYST+AP II+DLF KE RT +++ F FAIP+GSG+G+I+G  + T    W  ALR TP +G++ +F+L  +V EP RG+ +   +     TSW+ D+K L  NPSF+  T GFT V FV GSL+ +A        ++ G  +  +    D+S S+    IFG++TV+ G+ GV  G +A + LR   P ADPLVCA GL+L AP   LS++ A+     + V IF+ +T L  NWA+V D+ + VV P RR +A AFQ+++ H LGDA SPY +G++  S+ +     ++S    + S+Q ++L+ + + V GG FF+  + +I  D+  ++EN +
Sbjct:   44 DRRAIMTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSL----IFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKE-----SNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDR-DLAENYV 481          
BLAST of Spinster-like protein 1 vs. Ensembl Zebrafish
Match: spns2 (spinster homolog 2 (Drosophila) [Source:NCBI gene;Acc:568117])

HSP 1 Score: 345.895 bits (886), Expect = 3.739e-113
Identity = 188/458 (41.05%), Postives = 276/458 (60.26%), Query Frame = 2
Query:  158 GKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD--GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSK 1516
            G+ F AA    +   +   ++   N+LNYMDRYT+ G+L DI+  + + D  +GLLQT FI S+MV   +FGYLGDR NRK+I+ CG+ FWS +TL SSFI K+ +WL V+ R L+G+GE+SYS+++P II DLFT   RT +++ F  AIP+GSGLG+I+G   K   G W  ALR +P++G+    ++   V+EP RGSAD  G +    TSW+ D+K L  N S++F +L    V F  G+   +   Y+  A   +K+         S   + IFG++T + GL GVVIGA   R  R+    ADPLVCA  ++  A    L  V+AK     + + IFI +TLL  NWA+  D+ M VV P RR +A AFQ    H LGDAGSPY +G+I  SL + +   A+S    + S+ YA+++   ++V+GG FF+  + +  +D+ K ++ V + ++
Sbjct:   41 GQKFHAALLRCRTPLVAAGILSFGNVLNYMDRYTVAGVLLDIQKQFKVGDSSAGLLQTVFICSFMVAAPIFGYLGDRFNRKIILSCGIFFWSAVTLLSSFITKEYYWLLVLSRCLVGIGESSYSSISPTIIGDLFTNNKRTVMLSVFYLAIPLGSGLGYILGSIAKDAGGHWYWALRVSPMLGLTAGTLILIFVSEPKRGSADQPGGRLKTRTSWVCDMKALAKNRSYVFSSLASAAVSFATGAFGIWIPQYLVRAQVVQKSAESCTYQPCSSRDSLIFGAITCVTGLLGVVIGAVTTRLCRQKTERADPLVCAVSMLGSAIFICLIFVVAKKSIVGAYICIFIGETLLFLNWAITADILMYVVIPTRRATAVAFQGFTSHLLGDAGSPYLIGLISDSLQESY---ATSEIWQFLSLGYALMLCPFVIVLGGMFFLATALFFLDDRDKAAKQVNQLAR 495          
BLAST of Spinster-like protein 1 vs. Ensembl Zebrafish
Match: spns2 (spinster homolog 2 (Drosophila) [Source:NCBI gene;Acc:568117])

HSP 1 Score: 343.584 bits (880), Expect = 2.138e-112
Identity = 187/452 (41.37%), Postives = 272/452 (60.18%), Query Frame = 2
Query:  158 GKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD--GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSEN 1498
            G+ F AA    +   +   ++   N+LNYMDRYT+ G+L DI+  + + D  +GLLQT FI S+MV   +FGYLGDR NRK+I+ CG+ FWS +TL SSFI K+ +WL V+ R L+G+GE+SYS+++P II DLFT   RT +++ F  AIP+GSGLG+I+G   K   G W  ALR +P++G+    ++   V+EP RGSAD  G +    TSW+ D+K L  N S++F +L    V F  G+   +   Y+  A   +K+         S   + IFG++T + GL GVVIGA   R  R+    ADPLVCA  ++  A    L  V+AK     + + IFI +TLL  NWA+  D+ M VV P RR +A AFQ    H LGDAGSPY +G+I  SL + +   A+S    + S+ YA+++   ++V+GG FF+  + +  +D+ K ++ 
Sbjct:   41 GQKFHAALLRCRTPLVAAGILSFGNVLNYMDRYTVAGVLLDIQKQFKVGDSSAGLLQTVFICSFMVAAPIFGYLGDRFNRKIILSCGIFFWSAVTLLSSFITKEYYWLLVLSRCLVGIGESSYSSISPTIIGDLFTNNKRTVMLSVFYLAIPLGSGLGYILGSIAKDAGGHWYWALRVSPMLGLTAGTLILIFVSEPKRGSADQPGGRLKTRTSWVCDMKALAKNRSYVFSSLASAAVSFATGAFGIWIPQYLVRAQVVQKSAESCTYQPCSSRDSLIFGAITCVTGLLGVVIGAVTTRLCRQKTERADPLVCAVSMLGSAIFICLIFVVAKKSIVGAYICIFIGETLLFLNWAITADILMYVVIPTRRATAVAFQGFTSHLLGDAGSPYLIGLISDSLQESY---ATSEIWQFLSLGYALMLCPFVIVLGGMFFLATALFFLDDRDKAAKQ 489          
BLAST of Spinster-like protein 1 vs. Ensembl Xenopus
Match: spns2 (spinster homolog 2 [Source:NCBI gene;Acc:100145187])

HSP 1 Score: 341.273 bits (874), Expect = 4.024e-111
Identity = 188/456 (41.23%), Postives = 272/456 (59.65%), Query Frame = 2
Query:  143 DLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENV 1501
            +L S+      A+  ++       ++ + N+LNY+DRYT+ G+L DI+ ++ + D  +GLLQT FI S+MV   +FGYLGDR NRK+I+  G+ FWS IT +SSFIPK  FWL V+ R L+G+GEASYST+AP II DLFTK  RT +++ F FAIP+GSGLG+I G ++K   G W+ ALR +P++GV+   +L   V    RG A+ +K S   SWI D++ L+ N S++F +L  + V F  G+L  +  +Y+  A   +K+         S   + IFG++T L G  GV+IGA A +  RR    ADPLVCA G++  A    L  V AK     + + IF  +TLL  NWA+  DM M VV P RR +A A Q    H LGDAGSPY +G I   +      +  S+   + S+ YA+++   ++V+GG FF+  + +   D+ K  + V
Sbjct:   45 ELESSSSKPDKAYNWKRASVAAAGILSVGNVLNYLDRYTVAGVLLDIQQHFEVKDSGAGLLQTVFICSFMVAAPIFGYLGDRFNRKVILSSGIFFWSAITFSSSFIPKKYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQVAGDWRWALRVSPVLGVITGTLLLIFVPTAKRGHAEQLKGS---SWIRDMRGLIKNRSYVFSSLATSTVSFATGALGMWIPLYLYRAQVVQKSVEPCNIPPCSTKDSLIFGAITCLTGFLGVIIGAGATKWCRRKTQRADPLVCAVGMLGSAIFICLVFVAAKSSIIAAYICIFAGETLLFSNWAITADMLMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIQQS---TTKSSLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLEDREKAEKLV 494          
BLAST of Spinster-like protein 1 vs. Ensembl Xenopus
Match: spns2 (spinster homolog 2 [Source:NCBI gene;Acc:100145187])

HSP 1 Score: 340.117 bits (871), Expect = 1.195e-110
Identity = 187/454 (41.19%), Postives = 271/454 (59.69%), Query Frame = 2
Query:  143 DLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSE 1495
            +L S+      A+  ++       ++ + N+LNY+DRYT+ G+L DI+ ++ + D  +GLLQT FI S+MV   +FGYLGDR NRK+I+  G+ FWS IT +SSFIPK  FWL V+ R L+G+GEASYST+AP II DLFTK  RT +++ F FAIP+GSGLG+I G ++K   G W+ ALR +P++GV+   +L   V    RG A+ +K S   SWI D++ L+ N S++F +L  + V F  G+L  +  +Y+  A   +K+         S   + IFG++T L G  GV+IGA A +  RR    ADPLVCA G++  A    L  V AK     + + IF  +TLL  NWA+  DM M VV P RR +A A Q    H LGDAGSPY +G I   +      +  S+   + S+ YA+++   ++V+GG FF+  + +   D+ K  +
Sbjct:   45 ELESSSSKPDKAYNWKRASVAAAGILSVGNVLNYLDRYTVAGVLLDIQQHFEVKDSGAGLLQTVFICSFMVAAPIFGYLGDRFNRKVILSSGIFFWSAITFSSSFIPKKYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQVAGDWRWALRVSPVLGVITGTLLLIFVPTAKRGHAEQLKGS---SWIRDMRGLIKNRSYVFSSLATSTVSFATGALGMWIPLYLYRAQVVQKSVEPCNIPPCSTKDSLIFGAITCLTGFLGVIIGAGATKWCRRKTQRADPLVCAVGMLGSAIFICLVFVAAKSSIIAAYICIFAGETLLFSNWAITADMLMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIQQS---TTKSSLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLEDREKAEK 492          
BLAST of Spinster-like protein 1 vs. Ensembl Xenopus
Match: spns2 (spinster homolog 2 [Source:NCBI gene;Acc:100145187])

HSP 1 Score: 340.117 bits (871), Expect = 1.455e-110
Identity = 187/454 (41.19%), Postives = 271/454 (59.69%), Query Frame = 2
Query:  143 DLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSE 1495
            +L S+      A+  ++       ++ + N+LNY+DRYT+ G+L DI+ ++ + D  +GLLQT FI S+MV   +FGYLGDR NRK+I+  G+ FWS IT +SSFIPK  FWL V+ R L+G+GEASYST+AP II DLFTK  RT +++ F FAIP+GSGLG+I G ++K   G W+ ALR +P++GV+   +L   V    RG A+ +K S   SWI D++ L+ N S++F +L  + V F  G+L  +  +Y+  A   +K+         S   + IFG++T L G  GV+IGA A +  RR    ADPLVCA G++  A    L  V AK     + + IF  +TLL  NWA+  DM M VV P RR +A A Q    H LGDAGSPY +G I   +      +  S+   + S+ YA+++   ++V+GG FF+  + +   D+ K  +
Sbjct:   77 ELESSSSKPDKAYNWKRASVAAAGILSVGNVLNYLDRYTVAGVLLDIQQHFEVKDSGAGLLQTVFICSFMVAAPIFGYLGDRFNRKVILSSGIFFWSAITFSSSFIPKKYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQVAGDWRWALRVSPVLGVITGTLLLIFVPTAKRGHAEQLKGS---SWIRDMRGLIKNRSYVFSSLATSTVSFATGALGMWIPLYLYRAQVVQKSVEPCNIPPCSTKDSLIFGAITCLTGFLGVIIGAGATKWCRRKTQRADPLVCAVGMLGSAIFICLVFVAAKSSIIAAYICIFAGETLLFSNWAITADMLMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIQQS---TTKSSLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLEDREKAEK 524          
BLAST of Spinster-like protein 1 vs. Ensembl Xenopus
Match: cpne5 (copine V [Source:Xenbase;Acc:XB-GENE-956274])

HSP 1 Score: 312.383 bits (799), Expect = 1.117e-100
Identity = 176/397 (44.33%), Postives = 242/397 (60.96%), Query Frame = 2
Query:  323 LQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIP---TSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINY----IFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVS 1492
            L   FI SYM    +FGYLGDR NRKLI+  G++FWSL+TL SSF+ K  FWLF++ R L+GVGEASYST+AP II+DLF  + R+++++FF FA P+G GLG+I G  + +  G W  ALR TP +G+V + +L F+  EP RG+ +  K   P   TSW  D+K LL NPSF+  T GFT V FV G+L+ +   Y+  + +   +       +  NY    IFG +T + G+ GV+ G E  +R R+  P ADPLVCA G+I  AP   LS+  A      +   IFI +TLL  NWALV D+ + VV P RR +A A Q++V H LGDAGSPY +G+I   +       A S      S++YA++I A + VIGG FF+  + +I  D+ +  
Sbjct:   22 LPAVFICSYMFLAPVFGYLGDRYNRKLIMCIGISFWSLVTLLSSFVSKQYFWLFLLTRGLVGVGEASYSTIAPTIIADLFLADQRSRMLSFFYFATPVGCGLGYIAGSKVTSTAGDWHWALRVTPGLGLVAVLLLIFVAKEPPRGALER-KSDRPLTNTSWFSDVKALLKNPSFILSTFGFTTVAFVTGALALWGPTYLMRSRRVIYKTEPCQGGI-CNYDDSMIFGGITCVTGVLGVLTGVEISKRYRKTNPRADPLVCAVGMISSAPFLYLSLAFADTSLVATYAFIFIGETLLSLNWALVADILLYVVIPTRRSTAEALQIVVSHLLGDAGSPYLIGVISDQIQKG---KAPSFLIQMRSLEYALMICAFVGVIGGGFFLATALFIEKDRKRAE 413          
BLAST of Spinster-like protein 1 vs. Ensembl Xenopus
Match: spns2 (spinster homolog 2 [Source:NCBI gene;Acc:100145187])

HSP 1 Score: 304.679 bits (779), Expect = 4.188e-97
Identity = 181/468 (38.68%), Postives = 264/468 (56.41%), Query Frame = 2
Query:  143 DLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNID----DEKSGLLQT----------SFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSE 1495
            +L S+      A+  ++       ++ + N+LNY+DRYT+ G +  I G           ++ ++ T           FI S+MV   +FGYLGDR NRK+I+  G+ FWS IT +SSFIPK  FWL V+ R L+G+GEASYST+AP II DLFTK  RT +++ F FAIP+GSGLG+I G ++K   G W+ ALR +P++GV+   +L   V    RG A+ +K S   SWI D++ L+ N S++F +L  + V F  G+L  +  +Y+  A   +K+         S   + IFG++T L G  GV+IGA A +  RR    ADPLVCA G++  A    L  V AK     + + IF  +TLL  NWA+  DM M VV P RR +A A Q    H LGDAGSPY +G I   +      +  S+   + S+ YA+++   ++V+GG FF+  + +   D+ K  +
Sbjct:   44 ELESSSSKPDKAYNWKRASVAAAGILSVGNVLNYLDRYTVAGKV--ISGGRKCTVFMLRCRANIISTLYLDRVIELMIFICSFMVAAPIFGYLGDRFNRKVILSSGIFFWSAITFSSSFIPKKYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQVAGDWRWALRVSPVLGVITGTLLLIFVPTAKRGHAEQLKGS---SWIRDMRGLIKNRSYVFSSLATSTVSFATGALGMWIPLYLYRAQVVQKSVEPCNIPPCSTKDSLIFGAITCLTGFLGVIIGAGATKWCRRKTQRADPLVCAVGMLGSAIFICLVFVAAKSSIIAAYICIFAGETLLFSNWAITADMLMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFISDLIQQS---TTKSSLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLEDREKAEK 503          
BLAST of Spinster-like protein 1 vs. Ensembl Mouse
Match: Spns1 (spinster homolog 1 [Source:MGI Symbol;Acc:MGI:1920908])

HSP 1 Score: 363.614 bits (932), Expect = 9.020e-120
Identity = 200/453 (44.15%), Postives = 293/453 (64.68%), Query Frame = 2
Query:  203 IVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRIS-ASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            ++V V+  INLLNYMDR+T+ G+L DI+ ++NI D  +GL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP++ FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GV+ + +LF +V EP RG+ +   G     PTSW  D+K L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLRR  P ADPLVCA GL+  AP   L++  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I    +D+ R S   S  S + ++Q+++++ A +  +GG  F+  + +I +D+ +   +V      +  ++D
Sbjct:   60 LIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDVAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLSSLGFTSVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSAPFLFLALACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLI----SDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIEDDRRRAQLHVQGLLHESGPSDD 508          
BLAST of Spinster-like protein 1 vs. Ensembl Mouse
Match: Spns1 (spinster homolog 1 [Source:MGI Symbol;Acc:MGI:1920908])

HSP 1 Score: 344.354 bits (882), Expect = 2.914e-113
Identity = 185/387 (47.80%), Postives = 258/387 (66.67%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGII 1327
            +    ++V V+  INLLNYMDR+T+ G+L DI+ ++NI D  +GL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP++ FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GV+ + +LF +V EP RG+ +   G     PTSW  D+K L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLRR  P ADPLVCA GL+  AP   L++  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G++
Sbjct:   55 RGHSTLIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDVAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLSSLGFTSVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSAPFLFLALACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLV 441          
BLAST of Spinster-like protein 1 vs. Ensembl Mouse
Match: Spns2 (spinster homolog 2 [Source:MGI Symbol;Acc:MGI:2384936])

HSP 1 Score: 334.339 bits (856), Expect = 3.972e-108
Identity = 183/432 (42.36%), Postives = 262/432 (60.65%), Query Frame = 2
Query:  230 NLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD--GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADS---NYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENV 1501
            N+LNY+DRYT+ G+L DI+ ++ + D  +GLLQ+ FI S+MV   +FGYLGDR NRK+I+ CG+ FWS +T +SSFIP+  FWL V+ R L+G+GEASYST+AP II DLFTK  RT +++ F FAIP+GSGLG+I G ++K   G W  ALR +P++G++   ++  +V    RG AD  G +    TSW+ D+K L+ N S++F +L  + V F  G+L  +  +Y+  A   +K              + IFG++T   G  GVV GA A R  R     ADPLVCA G++  A    L  V AK     + + IF+ +TLL  NWA+  D+ M VV P RR +A A Q    H LGDAGSPY +G I    +D  R   S+ DS    + S+ YA+++   ++V+GG FF+  + +  +D+ K  + V
Sbjct:  112 NVLNYLDRYTVAGVLLDIQQHFGVKDRGAGLLQSVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTFSSSFIPQQYFWLLVLSRGLVGIGEASYSTIAPTIIGDLFTKNTRTLMLSVFYFAIPLGSGLGYITGSSVKQAAGDWHWALRVSPVLGMITGTLILILVPATKRGHADQLGGQLKARTSWLRDMKALIRNRSYVFSSLATSAVSFATGALGMWIPLYLHRAQVVQKTAETCNSPPCGAKDSLIFGAITCFTGFLGVVTGAGATRWCRLRTQRADPLVCAVGMLGSAIFICLIFVAAKTSIVGAYICIFVGETLLFSNWAITADILMYVVIPTRRATAVALQSFTSHLLGDAGSPYLIGFI----SDLIR--QSTKDSPLWEFLSLGYALMLCPFVVVLGGMFFLATALFFLSDRAKAEQQV 537          
BLAST of Spinster-like protein 1 vs. Ensembl Mouse
Match: Spns1 (spinster homolog 1 [Source:MGI Symbol;Acc:MGI:1920908])

HSP 1 Score: 321.627 bits (823), Expect = 4.053e-104
Identity = 184/449 (40.98%), Postives = 266/449 (59.24%), Query Frame = 2
Query:  203 IVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRIS-ASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            ++V V+  INLLNYMDR+T+ G+L DI+ ++NI D  +GL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP++ FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GV+ + +LF +V EP RG+ +   G     PTSW  D+K L  N                                                 IFG +T L G+ GV +G E  RRLRR  P ADPLVCA GL+  AP   L++  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I    +D+ R S   S  S + ++Q+++++ A +  +GG  F+  + +I +D+ +   +V      +  ++D
Sbjct:   60 LIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDVAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARN------------------------------------------------LIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSAPFLFLALACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLI----SDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIEDDRRRAQLHVQGLLHESGPSDD 456          
BLAST of Spinster-like protein 1 vs. Ensembl Mouse
Match: Spns3 (spinster homolog 3 [Source:MGI Symbol;Acc:MGI:1924827])

HSP 1 Score: 308.145 bits (788), Expect = 1.991e-98
Identity = 177/458 (38.65%), Postives = 267/458 (58.30%), Query Frame = 2
Query:  194 KDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIK----KSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYL---DNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADS---NYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            + ++   V+  INLLNYM+ + I G+L D++ Y++I D  +GLLQT FI   +V   +FGYLGDR NRK I+  G+  WS   L+SSFI     WLF + R  +G G ASYST+AP ++ DLF K+ RT  +A F   IP+GSGLG+++G  +    G W+ ALR  P +  + L +L  +V +  RG+A+       ++  +SW ED++ L  N SF+F TLG T + FV G+L ++A  ++  A   +   L           + IFG++TV  G+ GV++GAEA RR +++ P A+PL+CA  L   APC  L+++LA      S V + + + LL  NWA+V D+ +SVV P  RG+A A Q+ V H LGDAGSPY  G+I S L       A   DS   ++ S+Q++ L  A  +V+GG FF+  + ++  D+ +  +      K  + ++D
Sbjct:   47 RAYLAAAVLCYINLLNYMNWFIIPGVLLDVQKYFHISDSHAGLLQTVFISCLLVSAPVFGYLGDRYNRKAILSFGILLWSGAGLSSSFISYQYSWLFFLSRGFVGTGAASYSTIAPTVLGDLFVKDQRTCALAVFYIFIPVGSGLGYVLGSTVAELTGNWRWALRLMPCLDAMALALLILLVPDVPRGAAEKQGEVAVRAPRSSWCEDVRYLGRNWSFVFSTLGVTAIAFVTGALGFWAPKFLFEARVVHGLQLPCFQEQCHSQDSLIFGALTVATGIIGVMLGAEASRRYKKVNPRAEPLICASSLFATAPCLYLALILASRTLLASYVFLALGELLLSCNWAVVADILLSVVVPRCRGTAEALQITVAHVLGDAGSPYLTGLISSVLQ------AERPDSYLQHFLSLQHSFLCCAFAIVLGGGFFLLTALHLEKDQARARQP----GKGTLDSKD 494          
BLAST of Spinster-like protein 1 vs. UniProt/SwissProt
Match: sp|Q7ZU13|SPNS1_DANRE (Protein spinster homolog 1 OS=Danio rerio OX=7955 GN=spns1 PE=2 SV=1)

HSP 1 Score: 379.407 bits (973), Expect = 2.411e-125
Identity = 201/448 (44.87%), Postives = 289/448 (64.51%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAV-------IYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVI 1504
             ++  + V V+  INLLNYMDR+T+ G+LPDI+ ++ I D  SGLLQT FI SYM    LFGYLGDR NRKLI+  G+ FWS++TLASSFI KD FW  ++ R L+GVGEASYST+AP II+DLF KE RT +++ F FAIP+GSG+G+I+G  + T    W  ALR TP +G++ +F+L  +V EP RG+ +   +     TSW+ D+K L  NPSF+  T GFT V FV GSL+ +A        ++ G  +  +    D+S S+    IFG++TV+ G+ GV  G +A + LR   P ADPLVCA GL+L AP   LS++ A+     + V IF+ +T L  NWA+V D+ + VV P RR +A AFQ+++ H LGDA SPY +G++  S+ +     ++S    + S+Q ++L+ + + V GG FF+  + +I  D+  ++EN +
Sbjct:   44 DRRAIMTVIVLCYINLLNYMDRFTVAGVLPDIEHFFGIGDGTSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMCVGIFFWSVVTLASSFIGKDHFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGMGYIVGSKVDTVAKDWHWALRVTPGLGLLAVFLLMLVVQEPKRGAIEAHPEHTLHRTSWLADMKALCRNPSFILSTFGFTAVAFVTGSLALWAPAFLFRAGVFTGVKQPCFKAPCDDSDSL----IFGAITVVTGILGVASGVQASKLLRTRTPRADPLVCAAGLLLAAPFLYLSIMFAQASTVATYVFIFLGETFLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAISPYLIGVVSDSIKE-----SNSYMWEFRSLQMSLLLCSFVAVAGGAFFLATAVFIEKDR-DLAENYV 481          
BLAST of Spinster-like protein 1 vs. UniProt/SwissProt
Match: sp|Q5XGK0|SPNS1_XENLA (Protein spinster homolog 1 OS=Xenopus laevis OX=8355 GN=spns1 PE=2 SV=1)

HSP 1 Score: 378.252 bits (970), Expect = 1.349e-124
Identity = 204/443 (46.05%), Postives = 280/443 (63.21%), Query Frame = 2
Query:  191 KKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIP---TSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMG------YAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVS 1492
            K+  I+V ++F INLLNYMDR+T+ G+LPDIK  +NI D  SGL+QT FI SYM    +FGYLGDR NRKLI+  G++FWSL+TL SSF+    FWLF++ R L+GVGEASYST+AP II+DLF  + RT++++FF FA P+G GLG+I+G  + +  G W  ALR TP +G++ + +L F+  EP RG+ +  K   P   TSW  D+K LL NPSF+  T GFT V FV G+L+ +   Y+       Y  K     + N    + + IFG +T + G+ GV+ G E  +R R+  P ADPLVCA G+I  AP   LS+  A      + V IFI +TLL  NWALV D+ + VV P RR +A A Q++V H LGDAGSPY +G+I   +    +   +S      S++YA++I A + VIGG FF+  + +I  D+ K  
Sbjct:   56 KRSVIIVIILFYINLLNYMDRFTVAGVLPDIKKAFNISDSNSGLVQTVFICSYMFLAPVFGYLGDRYNRKLIMCVGISFWSLVTLLSSFVSNQYFWLFLITRGLVGVGEASYSTIAPTIIADLFLADQRTRMLSFFYFATPVGCGLGYIVGSEMTSAAGDWHWALRVTPGLGLLAVLLLIFVAEEPPRGALER-KTDRPLTNTSWSSDMKALLKNPSFILSTFGFTTVAFVTGALALWGPTYLMRSRMVIYKSKPCEGGICN---YDDSMIFGGITCITGILGVLTGVEISKRYRKTNPRADPLVCAVGMISSAPFLFLSLAFADTSLVATYVFIFIGETLLSLNWALVADILLYVVIPTRRSTAEALQIVVSHLLGDAGSPYLIGVISDQIQ---KGKPASFLIQMRSLEYALMICAFVGVIGGGFFLTTALFIEKDRKKAE 491          
BLAST of Spinster-like protein 1 vs. UniProt/SwissProt
Match: sp|A8WGF7|SPNS1_XENTR (Protein spinster homolog 1 OS=Xenopus tropicalis OX=8364 GN=spns1 PE=2 SV=2)

HSP 1 Score: 369.777 bits (948), Expect = 2.501e-121
Identity = 203/466 (43.56%), Postives = 282/466 (60.52%), Query Frame = 2
Query:  128 GLSESDLSSNGKSFRAAFKL-----QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIP---TSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSM---NINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVS 1492
            G S  +    GK       L      +    +V ++F INLLNYMDR+T+ G+L DIK  Y+I D  SGL+QT FI SYM    +FGYLGDR NRKLI+  G++FWSL+TL SSF+ K  FWLF++ R L+GVGEASYST+AP II+DLF  + R+++++FF FA P+G GLG+I G  + +  G W  ALR TP +G+V + +L F+  EP RG+ +  K   P   TSW  D+K LL NPSF+  T GFT V FV G+L+ +   Y+  + +   +       +   + + IFG +T + G+ GV+ G E  +R R+  P ADPLVCA G+I  AP   LS+  A      +   IFI +TLL  NWALV D+ + VV P RR +A A Q++V H LGDAGSPY +G+I   +    +  A S      S++YA++I A + VIGG FF+  + +I  D+ +  
Sbjct:   30 GHSSDEEEEEGKDHGKEAHLLTGISYRHSVTIVIILFYINLLNYMDRFTVAGVLSDIKEDYHISDSNSGLVQTVFICSYMFLAPVFGYLGDRYNRKLIMCIGISFWSLVTLLSSFVSKQYFWLFLLTRGLVGVGEASYSTIAPTIIADLFLADQRSRMLSFFYFATPVGCGLGYIAGSKVTSTAGDWHWALRVTPGLGLVAVLLLIFVAKEPPRGALER-KSDRPLTNTSWFSDVKALLKNPSFILSTFGFTTVAFVTGALALWGPTYLMRSRRVIYKTEPCQGGICNYDDSMIFGGITCVTGVLGVLTGVEISKRYRKTNPRADPLVCAVGMISSAPFLYLSLAFADTSLVATYAFIFIGETLLSLNWALVADILLYVVIPTRRSTAEALQIVVSHLLGDAGSPYLIGVISDQIQ---KGKAPSFLIQMRSLEYALMICAFVGVIGGGFFLATALFIEKDRKRAE 491          
BLAST of Spinster-like protein 1 vs. UniProt/SwissProt
Match: sp|Q2YDU8|SPNS1_RAT (Protein spinster homolog 1 OS=Rattus norvegicus OX=10116 GN=Spns1 PE=2 SV=2)

HSP 1 Score: 367.081 bits (941), Expect = 3.779e-120
Identity = 201/453 (44.37%), Postives = 292/453 (64.46%), Query Frame = 2
Query:  203 IVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRIS-ASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            ++V V+  INLLNYMDR+T+ G+L DI+ ++NI D  +GL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP++ FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GV+ + +LF +V EP RG+ +   G     PTSW  D+K L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLRR  P ADPLVCA GL+  +P   LS+  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I    +D+ R S   S  S + ++Q+++++ A +  +GG  F+  + +I ND+ +   +V         ++D
Sbjct:   60 LIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDLAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSSPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLI----SDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIENDRRRAQLHVQGLLHETEPSDD 508          
BLAST of Spinster-like protein 1 vs. UniProt/SwissProt
Match: sp|Q8R0G7|SPNS1_MOUSE (Protein spinster homolog 1 OS=Mus musculus OX=10090 GN=Spns1 PE=2 SV=1)

HSP 1 Score: 363.614 bits (932), Expect = 6.309e-119
Identity = 200/453 (44.15%), Postives = 293/453 (64.68%), Query Frame = 2
Query:  203 IVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLD----NSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRIS-ASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNED 1537
            ++V V+  INLLNYMDR+T+ G+L DI+ ++NI D  +GL+QT FI SYMV   +FGYLGDR NRK ++  G+ FWSL+TL SSFIP++ FWL ++ R L+GVGEASYST+AP +I+DLF  + R+++++ F FAIP+GSGLG+I G  +K   G W  ALR TP +GV+ + +LF +V EP RG+ +   G     PTSW  D+K L  NPSF+  +LGFT V FV GSL+ +A  ++  +     +       +S S + + IFG +T L G+ GV +G E  RRLRR  P ADPLVCA GL+  AP   L++  A+     + + IFI +TLL  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G+I    +D+ R S   S  S + ++Q+++++ A +  +GG  F+  + +I +D+ +   +V      +  ++D
Sbjct:   60 LIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDVAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLSSLGFTSVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSAPFLFLALACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLI----SDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIEDDRRRAQLHVQGLLHESGPSDD 508          
BLAST of Spinster-like protein 1 vs. TrEMBL
Match: A0A1S3JSB8 (protein spinster homolog 1 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106175696 PE=4 SV=1)

HSP 1 Score: 414.846 bits (1065), Expect = 2.578e-136
Identity = 223/504 (44.25%), Postives = 327/504 (64.88%), Query Frame = 2
Query:   98 NSLVLSRSWKGLSESDLSSNGK------------SFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYM-------GYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIF-PYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASS-ADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARI 1546
            N +    + K +S+  L  NG+            S R     + + +++V V+  INLLNYMDR+TI G+L  I+ YY +DD+ SGLLQT FI+SYM+   LFGYLGDR NRK+I++ G+ FWS ITLASSF+P +MF LF+ +R  +G+GEASYST+AP +I+DLF K+ RTK++A F FAIPIGSGLG+I+G  + T  G W+ ALR TP++GV+C+ ++  +V EP RG A+G      TS+++D+K LL N SF++ TLGFT V FV G+L+ +A  Y+          ++   Q  D SSS+++   FG +T LAG FGV IGAE+ RRLR  F   ADP VCAFGL+   P   +++V+++Y+   + V+IF+ +TLLC NW++V D+ + VV P RR +A A Q+++ H LGDAGSPY +G+    ++DK R +        Y+++QYA+ I A + V+GG  F+  + YI  DK K  + + +  ++ +S E+  +
Sbjct:   29 NGIFPDDTEKIISDGRLEGNGREDPDDSVEVKIPSERPHNITKARSYVIVAVLLFINLLNYMDRFTIAGVLTKIRPYYQLDDQLSGLLQTVFIVSYMLLSPLFGYLGDRYNRKIIMVAGICFWSGITLASSFVPANMFGLFLFLRGCVGIGEASYSTIAPTLIADLFVKDMRTKMLAIFYFAIPIGSGLGYIVGSEIATALGNWKWALRVTPVLGVICVILISTLVKEPPRGQAEGGTHLHSTSYLDDLKYLLKNKSFVWSTLGFTSVAFVTGALALWAPTYVYNSILVQDQVDRTPKQKDDLSSSVSLT--FGIITCLAGFFGVGIGAESARRLRSKFDARADPFVCAFGLLSCMPFLYVAIVVSQYNTIVTWVLIFLGETLLCLNWSIVADILLGVVIPTRRSTAEAVQIVMSHLLGDAGSPYLIGL----MSDKIRGNEPKYPQYEYFALQYALYITAFVAVLGGGAFLATAIYIVYDKKKTDKIIHEGLQAEVSTEEDTV 526          
BLAST of Spinster-like protein 1 vs. TrEMBL
Match: K1QTC7 (Spinster-like protein 1 OS=Crassostrea gigas OX=29159 GN=CGI_10010034 PE=4 SV=1)

HSP 1 Score: 414.846 bits (1065), Expect = 2.792e-136
Identity = 211/463 (45.57%), Postives = 308/463 (66.52%), Query Frame = 2
Query:  131 LSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKF----RISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIK 1507
            ++ES + +      A    + + + +VFV+  INLLNYMDR+TI G+L DI+ YY+I + ++GL+QT FI+SYM+   +FGYLGDR NRK I+  G+T WSL+TL+ SFI KD FW F+++R+ +G+GEASYST+AP II+DLF K+ RT+++  F FAIP+GSGLG+++G  +   FG WQ ALRFTP++G++C+ ++F ++ EP RG A+G      +S+++D+K+L    SF+  TLGFT V FV G+L+ +A  YM    KA  Q  D     NI +IFG +TV AG  GV +GAEA RR +RI P ADPLVCAFGL++  P     + L++ +   S  +IF  +T LC NW+++ D+ + VV P RR +A A Q+++ HALGDAGSPY +G+I  +L  K+    R+S S   S   +++ A  I   + VIGG FF+  + +I  D+ K +E + K
Sbjct:   33 IAESHVRTPDVEVVAGDISKHRAYFMVFVLLFINLLNYMDRFTIAGVLVDIQNYYHIGNSEAGLIQTVFIVSYMIFSPIFGYLGDRYNRKFIMGGGITLWSLLTLSGSFIGKDHFWAFILIRAAVGIGEASYSTIAPTIIADLFAKDLRTRMLMVFYFAIPVGSGLGYVVGANIAKAFGAWQWALRFTPVLGIICVILIFIVLKEPQRGHAEGGSHLRNSSYLQDLKELAKTKSFVLSTLGFTCVAFVTGALALWAPSYMFNVIKAQGQDADEG---NIAFIFGGITVAAGFIGVALGAEASRRYKRINPRADPLVCAFGLLMCTPFLFFGLWLSESNIPVSWALIFFGETFLCLNWSIIADILLYVVIPTRRSTAEAVQILISHALGDAGSPYLIGVISDALATKYPKDERLSPSVESS---TLRNAFYITPFVCVIGGGFFLATALFIQKDREK-AEKITK 488          
BLAST of Spinster-like protein 1 vs. TrEMBL
Match: A0A2R2MTX2 (protein spinster homolog 1 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106175696 PE=4 SV=1)

HSP 1 Score: 414.075 bits (1063), Expect = 1.590e-135
Identity = 225/502 (44.82%), Postives = 325/502 (64.74%), Query Frame = 2
Query:   98 NSLVLSRSWKGLSESDLSSNGK------------SFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYM-------GYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIF-PYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASS-ADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSE---NVIKYSKSNMSN 1531
            N +    + K +S+  L  NG+            S R     + + +++V V+  INLLNYMDR+TI G+L  I+ YY +DD+ SGLLQT FI+SYM+   LFGYLGDR NRK+I++ G+ FWS ITLASSF+P +MF LF+ +R  +G+GEASYST+AP +I+DLF K+ RTK++A F FAIPIGSGLG+I+G  + T  G W+ ALR TP++GV+C+ ++  +V EP RG A+G      TS+++D+K LL N SF++ TLGFT V FV G+L+ +A  Y+          ++   Q  D SSS+++   FG +T LAG FGV IGAE+ RRLR  F   ADP VCAFGL+   P   +++V+++Y+   + V+IF+ +TLLC NW++V D+ + VV P RR +A A Q+++ H LGDAGSPY +G+    ++DK R +        Y+++QYA+ I A + V+GG  F+  + YI  DK K  +     I+ S S+ +N
Sbjct:   29 NGIFPDDTEKIISDGRLEGNGREDPDDSVEVKIPSERPHNITKARSYVIVAVLLFINLLNYMDRFTIAGVLTKIRPYYQLDDQLSGLLQTVFIVSYMLLSPLFGYLGDRYNRKIIMVAGICFWSGITLASSFVPANMFGLFLFLRGCVGIGEASYSTIAPTLIADLFVKDMRTKMLAIFYFAIPIGSGLGYIVGSEIATALGNWKWALRVTPVLGVICVILISTLVKEPPRGQAEGGTHLHSTSYLDDLKYLLKNKSFVWSTLGFTSVAFVTGALALWAPTYVYNSILVQDQVDRTPKQKDDLSSSVSLT--FGIITCLAGFFGVGIGAESARRLRSKFDARADPFVCAFGLLSCMPFLYVAIVVSQYNTIVTWVLIFLGETLLCLNWSIVADILLGVVIPTRRSTAEAVQIVMSHLLGDAGSPYLIGL----MSDKIRGNEPKYPQYEYFALQYALYITAFVAVLGGGAFLATAIYIVYDKKKTDKIIHEAIQLSNSSPTN 524          
BLAST of Spinster-like protein 1 vs. TrEMBL
Match: A0A3P8VVS1 (Spinster homolog 1 (Drosophila) OS=Cynoglossus semilaevis OX=244447 PE=4 SV=1)

HSP 1 Score: 403.29 bits (1035), Expect = 4.484e-132
Identity = 211/472 (44.70%), Postives = 296/472 (62.71%), Query Frame = 2
Query:  134 SESDLSSNGKSFRAAFKLQKKD---------FIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADS---NYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSEN 1498
            SE++   +G S   A   Q++           + VF++  INLLNYMDR+T+ G+LPDI+ Y+ IDD KSGLLQT FI SYM    LFGYLGDR NRKLI+ CG+TFWSL+TLASS+ P+D FWL ++ R L+GVGEASYST+AP II+DL+ KE RT +++ F FAIP+GSGLG+I+G  +      W +ALR TP +G+V + +L F+V EP RG+ +   +     TSW+ D++ L  N SF+  TLGFT V FV GSL+ +A  ++  A          +D   S   + IFGS+T + G+ GV  G    R LR++ P ADPLVCA GL++ AP   L++V A+     + V IF+ +T L  NWA+V D+ + VV P RR +A A Q+++ H LGDAGSPY +G++  SL           DS    + S+Q ++L+ A + VIGG FF+  + +I  D+ +  + 
Sbjct:   23 SETEPQEDGGSSTVAQGPQEEQPSGVSKVRALLTVFILCYINLLNYMDRFTVAGVLPDIENYFQIDDGKSGLLQTVFICSYMFLAPLFGYLGDRYNRKLIMSCGITFWSLVTLASSYTPRDYFWLLLLTRGLVGVGEASYSTIAPTIIADLYVKEKRTNMLSIFYFAIPVGSGLGYIVGSQVGRIANDWHSALRVTPALGLVAVVLLIFVVQEPKRGAIEARPEHQLHRTSWMTDLQVLSGNCSFVLSTLGFTAVAFVTGSLALWAPTFLLRATIFSGVKAPCVDGHCSTTDSLIFGSITCVTGVLGVASGVLVSRHLRKVTPRADPLVCAAGLLMSAPFLYLAIVFAETSIVATYVFIFLGETFLSMNWAIVADILLYVVVPTRRSTAEALQIVISHLLGDAGSPYVIGVVSDSLR--------RTDSYLWQFRSLQLSLLLCAFVAVIGGAFFLATALFIERDRHRAEKT 486          
BLAST of Spinster-like protein 1 vs. TrEMBL
Match: T1J6E4 (MFS domain-containing protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 400.593 bits (1028), Expect = 1.239e-130
Identity = 198/440 (45.00%), Postives = 289/440 (65.68%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIK 1507
             K     V ++F +NLLNYMDR+TI G+LP+++ +YNID  ++GLLQTSF+ S+M+   +FGYLGDR NRK I+  G+ FWSL TL  SFIP   FW F+  R L+G+GEASYST+AP II+D++    R++++AFF FAIP+GSG+G+++G  +   F  W  ALR TP+ G++C+ +L F++ EP RG ++G     PT W ED+  LL NPSF++ T GFT V FV G+L+W+   ++ YA K   Q  D  +   I+ +FG +TV+AG+ GV  G+ A + L++    ADPLVCAFG+I   P      ++A+++   +  +IF  +  LC NWALV D+ + VV P RR SA AFQ+++ HALGDAGSPY +G I  ++ D    +   A   ++SMQ A+     + V+GG  FI  S+YI +DK K ++++I+
Sbjct:   89 SKSALATVLILFFVNLLNYMDRFTIAGVLPEVQTFYNIDHTEAGLLQTSFVCSFMILAPIFGYLGDRYNRKYIMAAGILFWSLTTLIGSFIPAGYFWWFLFFRGLVGIGEASYSTIAPTIIADMYKGTMRSRMLAFFYFAIPVGSGMGYMVGSNVAKAFQSWHWALRVTPVFGIICVLLLIFVLKEPPRGESEGGHDLAPTLWKEDMISLLHNPSFIWSTFGFTAVAFVAGALAWWGPSFLLYAVKVQKQTTDLGT---ISLVFGVITVVAGIIGVTAGSLAAQMLKKHDMTADPLVCAFGMITSMPFLFFGAIVAEHYTAAAYTLIFFGEVFLCLNWALVADILLYVVVPTRRSSAEAFQILLSHALGDAGSPYIIGTIADAIGDGKSPTNFMA---FHSMQCALFTTCFVSVLGGLCFIICSWYIIDDK-KKADDIIR 521          
BLAST of Spinster-like protein 1 vs. Ensembl Cavefish
Match: spns1 (sphingolipid transporter 1 (putative) [Source:NCBI gene;Acc:103032180])

HSP 1 Score: 370.163 bits (949), Expect = 6.545e-123
Identity = 204/451 (45.23%), Postives = 281/451 (62.31%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF-------AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAP---ILVIGGFFFIFASFYITNDKFKVSENVI 1504
            + + F+ V V+  INLLNYMDR+T+ G+LPDI+ +++I D KSGLLQT FI SYM+   LFGYLGDR NRK+I+  G+ FWS+ITLASSFI KD FW  ++ R L+GVGEASYST+AP II+DLF KE RT +++ F FAIP+GSGLG+I+G A+      W  ALR TP +GVV +F+L  +V EP RG+ +   +     TSW+ D+K L  N SF+  T GFT V FV GSL+ +       A ++ G       +  D S S+     FG +T ++G+ GV +G    + LR+  P ADPLVCA GL+L AP   LS++ A+     +   IF+ +T L  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G++  SL          ADS  +  +   L       + V GG FF+  + +I  D+ + +EN I
Sbjct:   47 KTRAFLTVGVLCYINLLNYMDRFTVAGVLPDIEHFFSISDGKSGLLQTVFICSYMLLAPLFGYLGDRYNRKMIMCFGIAFWSIITLASSFIAKDYFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGLGYIVGSAVNDRAKDWHWALRVTPALGVVAVFLLVCVVKEPKRGAIEARPEHTLHRTSWLADMKALCQNKSFILSTFGFTAVAFVTGSLALWAPAFLFRAAVFTGEKVPCVKEPCDTSDSL----YFGVITCISGVLGVSMGVGISQYLRKRTPRADPLVCAAGLLLAAPFLYLSIIFAQKSTIATYFFIFLGETFLSMNWAIVADILLYVVVPTRRSTAEAFQIVLSHLLGDAGSPYLIGVVSDSLKQ--------ADSYMWEFRSLQLSLLLCSFVAVGGGAFFLATALFIEKDRHR-AENYI 484          
BLAST of Spinster-like protein 1 vs. Ensembl Cavefish
Match: spns1 (sphingolipid transporter 1 (putative) [Source:NCBI gene;Acc:103032180])

HSP 1 Score: 370.548 bits (950), Expect = 1.025e-122
Identity = 204/451 (45.23%), Postives = 281/451 (62.31%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF-------AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAP---ILVIGGFFFIFASFYITNDKFKVSENVI 1504
            + + F+ V V+  INLLNYMDR+T+ G+LPDI+ +++I D KSGLLQT FI SYM+   LFGYLGDR NRK+I+  G+ FWS+ITLASSFI KD FW  ++ R L+GVGEASYST+AP II+DLF KE RT +++ F FAIP+GSGLG+I+G A+      W  ALR TP +GVV +F+L  +V EP RG+ +   +     TSW+ D+K L  N SF+  T GFT V FV GSL+ +       A ++ G       +  D S S+     FG +T ++G+ GV +G    + LR+  P ADPLVCA GL+L AP   LS++ A+     +   IF+ +T L  NWA+V D+ + VV P RR +A AFQ+++ H LGDAGSPY +G++  SL          ADS  +  +   L       + V GG FF+  + +I  D+ + +EN I
Sbjct:   47 KTRAFLTVGVLCYINLLNYMDRFTVAGVLPDIEHFFSISDGKSGLLQTVFICSYMLLAPLFGYLGDRYNRKMIMCFGIAFWSIITLASSFIAKDYFWALLLTRGLVGVGEASYSTIAPTIIADLFVKEKRTNMLSIFYFAIPVGSGLGYIVGSAVNDRAKDWHWALRVTPALGVVAVFLLVCVVKEPKRGAIEARPEHTLHRTSWLADMKALCQNKSFILSTFGFTAVAFVTGSLALWAPAFLFRAAVFTGEKVPCVKEPCDTSDSL----YFGVITCISGVLGVSMGVGISQYLRKRTPRADPLVCAAGLLLAAPFLYLSIIFAQKSTIATYFFIFLGETFLSMNWAIVADILLYVVVPTRRSTAEAFQIVLSHLLGDAGSPYLIGVVSDSLK--------QADSYMWEFRSLQLSLLLCSFVAVGGGAFFLATALFIEKDRHR-AENYI 484          
BLAST of Spinster-like protein 1 vs. Ensembl Cavefish
Match: SPNS2 (sphingolipid transporter 2 [Source:NCBI gene;Acc:103026912])

HSP 1 Score: 335.109 bits (858), Expect = 5.712e-109
Identity = 182/425 (42.82%), Postives = 264/425 (62.12%), Query Frame = 2
Query:  230 NLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIP--TSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAE----KAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFK 1486
            N+LNY+DRYT+ G++ DIK +Y + D   GLLQT FI S+MV   +FGYLGDR NRK+I+ CG+ FWS +TL SSFI K+ +WLFV+ R L+G+GE+SYS+++P II DLF+   RT +++ F  AIP+GSGLG+I+G + K   G W  ALR +P++G++   ++   V EP RGS+D     +   TSW+ D+K L  N S++F +L    V F  G+   +   Y+  A+     A +  +   SS + + IFG+MT + GL GVVIGA   R  R+    ADPLVCA  ++  A    L  V+AK     + + IFI +TLL  NWA+  D+ M VV P RR +A AFQ    H LGDAGSPY +G+I  +L   +   A+S    + S+ YA+++   ++V+GG FF+  + +  +D+ K
Sbjct:   67 NVLNYLDRYTVAGVMLDIKQHYKVSDSGVGLLQTVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTLLSSFITKEYYWLFVLSRGLVGIGESSYSSISPTIIGDLFSSNSRTIMLSVFYLAIPLGSGLGYILGSSAKDAAGDWHWALRVSPLLGMIAGMLILLFVPEPKRGSSDQPSSRMKSRTSWVCDMKALGKNRSYVFSSLASAAVSFATGAFGIWIPQYLFRAQVVQKTAVSCTVQPCSSRD-SLIFGAMTCVTGLLGVVIGAATTRFCRQKTERADPLVCAVSMLGSAIFICLIFVVAKKSIVGAYICIFIGETLLFLNWAITADILMYVVIPTRRATAVAFQGFTSHLLGDAGSPYLIGLISDALQKSY---ATSVLWQFLSLGYALMLCPFVIVLGGMFFLATALFYLDDRDK 487          
BLAST of Spinster-like protein 1 vs. Ensembl Cavefish
Match: spns3 (spinster homolog 3 (Drosophila) [Source:NCBI gene;Acc:568387])

HSP 1 Score: 305.064 bits (780), Expect = 2.677e-98
Identity = 178/420 (42.38%), Postives = 267/420 (63.57%), Query Frame = 2
Query:  263 TGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD--GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIK 1507
            TG+L  I+ Y+NI D  SGLLQT FI S+M+   +FGYLGDR NRK+I++ G+  W + TL+SSF+ +  FWL +V+R+L+G GEASY T+AP II D+FT   RT +++FF   IP+GSGLG+I+G  + +  G W+ ALR  PI+G + L +L F++  P RG+++  G      T+++EDIK LL N SF++ +LG T + FV G+L+++   ++  A   +   N         + +YIFG++TV+ G+ GV IG    RRLR     ADPL+CA G++  +PC  +++VLA      +   I I +TLL  NWA++ D+ + VV P RR +A A Q++V H LGDAGSPY +G I  +L+    +   S D  + S++Y++L+   I V+GG FF+  + YI  DK K +E ++K
Sbjct:   10 TGVLLSIQKYFNISDSTSGLLQTVFICSFMLLAPVFGYLGDRYNRKVIMVAGMVVWIVTTLSSSFVTESYFWLLIVLRALVGTGEASYVTIAPTIIGDMFTGTKRTLMISFFYIFIPVGSGLGYILGATIASATGDWRWALRLNPILGGLGLVLLIFLIPNPPRGASETQGATCMERTTYLEDIKYLLRNRSFVWSSLGVTAMAFVTGALAFWTPTFLSRARVIQGLTNPCTKEPCDPSDSYIFGAITVVTGVVGVFIGTYISRRLRDTVANADPLICAVGMLSSSPCLFIAIVLASTSIPATYACIGIGETLLSLNWAILADILLCVVIPTRRATAEALQIMVCHLLGDAGSPYLLGAISDALST---VKPDSPDWRFRSLEYSVLLCPFIGVLGGLFFLLTALYIKADK-KAAEMLMK 425          
BLAST of Spinster-like protein 1 vs. Ensembl Cavefish
Match: SPNS2 (sphingolipid transporter 2 [Source:NCBI gene;Acc:103026912])

HSP 1 Score: 306.99 bits (785), Expect = 4.360e-98
Identity = 176/426 (41.31%), Postives = 257/426 (60.33%), Query Frame = 2
Query:  230 NLLNYMDRYTITGLLPDIKGYYNIDDEKSGL-LQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIP--TSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAE----KAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFK 1486
            N+LNY+DRYT+ G   +   +  ID   + L L   FI S+MV   +FGYLGDR NRK+I+ CG+ FWS +TL SSFI K+ +WLFV+ R L+G+GE+SYS+++P II DLF+   RT +++ F  AIP+GSGLG+I+G + K   G W  ALR +P++G++   ++   V EP RGS+D     +   TSW+ D+K L  N S++F +L    V F  G+   +   Y+  A+     A +  +   SS + + IFG+MT + GL GVVIGA   R  R+    ADPLVCA  ++  A    L  V+AK     + + IFI +TLL  NWA+  D+ M VV P RR +A AFQ    H LGDAGSPY +G+I  +L   +   A+S    + S+ YA+++   ++V+GG FF+  + +  +D+ K
Sbjct:   67 NVLNYLDRYTVAGKRDNT--HTLIDAAAASLFLCVVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSAVTLLSSFITKEYYWLFVLSRGLVGIGESSYSSISPTIIGDLFSSNSRTIMLSVFYLAIPLGSGLGYILGSSAKDAAGDWHWALRVSPLLGMIAGMLILLFVPEPKRGSSDQPSSRMKSRTSWVCDMKALGKNRSYVFSSLASAAVSFATGAFGIWIPQYLFRAQVVQKTAVSCTVQPCSSRD-SLIFGAMTCVTGLLGVVIGAATTRFCRQKTERADPLVCAVSMLGSAIFICLIFVVAKKSIVGAYICIFIGETLLFLNWAITADILMYVVIPTRRATAVAFQGFTSHLLGDAGSPYLIGLISDALQKSY---ATSVLWQFLSLGYALMLCPFVIVLGGMFFLATALFYLDDRDK 486          
BLAST of Spinster-like protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008246.1 (pep scaffold:Pmarinus_7.0:GL480278:6713:20715:1 gene:ENSPMAG00000007448.1 transcript:ENSPMAT00000008246.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 339.732 bits (870), Expect = 4.915e-112
Identity = 183/443 (41.31%), Postives = 271/443 (61.17%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSAD---GIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSS-----MNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVS 1492
            + +   VV ++F +NLLNYMDR+T+ G       Y  +    S      FI SYM+   +FGYLGDR NRK+I+  G+  WS+ TL SSFIPK+ FW F++MR L+G+GEASYST+AP +I+DL+T++ RT++++ F FAIP+GSGLG+I+G  L    G WQ  LR TP++GV  + ++FF+V EP RG+ +   G+  S  TSW+ D+K L  N S ++ +LGFT V FV G+L+ ++  ++  AE    +     +        ++ IFG +T ++G  GV +GAE  RR ++    ADPLVCAFGL+  AP   LS+V A+ +   +   IF  +TLLC NWA+V D+ + VV P RR +A + Q+++ H LGDA SPY +G I    +D  +    S    + S++YA+LI A +   GG  F+  +  I  D+ +  
Sbjct:    6 RHRSLAVVLILFYVNLLNYMDRFTVAGARQTKAQYCIVLQWSSSKSCAVFICSYMLLAPIFGYLGDRFNRKMIMSVGIAVWSVTTLGSSFIPKENFWAFLLMRGLVGIGEASYSTIAPTVIADLYTRDKRTRMLSIFYFAIPVGSGLGYILGANLAQLTGNWQWGLRVTPVLGVFAVLLIFFVVPEPPRGAIEQETGVHLS-RTSWLTDLKCLARNQSLLWSSLGFTSVSFVTGALALWSPEFLSLAEVVQGRLRPCVTEPCEGYSQVSLIFGVLTCVSGFIGVGVGAEIARRYKKYNARADPLVCAFGLLSSAPFLFLSLVTAESNTTATYFFIFCGETLLCLNWAIVADILLYVVIPTRRSTAESVQILMSHLLGDAFSPYLIGKI----SDSIKGDRDSYLWRFLSLEYALLICAFVCTFGGACFLKTATVIEQDRERAE 443          
BLAST of Spinster-like protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006804.1 (pep scaffold:Pmarinus_7.0:GL479580:1268:6755:1 gene:ENSPMAG00000006130.1 transcript:ENSPMAT00000006804.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 236.884 bits (603), Expect = 8.792e-74
Identity = 139/342 (40.64%), Postives = 207/342 (60.53%), Query Frame = 2
Query:  260 ITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRG------SADGIKKSIP-TSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYM-------GYAEKAYNQYLDN-SSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTM-SVVN 1237
            ITG+L +I+ ++NID+  SGL+   F+ SYM+  +LFG+LGDR  RK I+   +  WS  TL  SF+ +  F  F+V R+L+GVGEA YSTVA  I+ DLF    RT+++A +  AIPIGSGLG+I+G  +    G W+ ALR TP +G++   ++  +V EP RG      S  G   + P T+W  D++ L  N S +  +LGFT V FV G+L+ ++ +Y+       G  +   +Q     SS+ +++ +FG++T  AGL GV +G+EA RR RR  P A+PLVC  GL+   P    +++LAK     + V IF+ + LL  NWAL+ D+ +  V+N
Sbjct:    1 ITGVLAEIQTFFNIDNSLSGLIPAVFLCSYMILALLFGFLGDRYPRKFIMAASILVWSGTTLGGSFVREKEFTGFLVSRALVGVGEAGYSTVAATILGDLFVGSTRTRVLALYYSAIPIGSGLGYIVGSKIAEVTGDWRNALRVTPGLGLLACVLVVLVVVEPERGAVERHSSGGGGPAARPRTTWFTDVRMLCSNRSLVLSSLGFTCVSFVSGALALWSPLYLELAGVVRGSLQPCEHQPCPGFSSAGSVSMVFGALTCSAGLAGVALGSEAARRWRRCEPSAEPLVCGLGLLASGPFLYAALLLAKSSPVTTYVFIFLGEVLLFLNWALIADILLYGVIN 342          
BLAST of Spinster-like protein 1 vs. Ensembl Yeast
Match: HXT2 (High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose [Source:SGD;Acc:S000004613])

HSP 1 Score: 46.9802 bits (110), Expect = 5.008e-6
Identity = 29/125 (23.20%), Postives = 60/125 (48.00%), Query Frame = 2
Query:  287 GYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTW 661
            G Y + D ++GL+   F +      +  G LGD   R++ +MC V  + ++ +       D ++ + + R + G+G    + ++P +IS+   K  R   ++F+   I +G  LG+   +  K +
Sbjct:   97 GTYYLSDVRTGLIVGIFNIGCAFGGLTLGRLGDMYGRRIGLMCVVLVY-IVGIVIQIASSDKWYQYFIGRIISGMGVGGIAVLSPTLISETAPKHIRGTCVSFYQLMITLGIFLGYCTNYGTKDY 220          
BLAST of Spinster-like protein 1 vs. Ensembl Nematostella
Match: EDO39433 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SA53])

HSP 1 Score: 354.755 bits (909), Expect = 1.062e-116
Identity = 196/485 (40.41%), Postives = 290/485 (59.79%), Query Frame = 2
Query:  134 SESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEK-SGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRG-----------SADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILT 1552
            + S  S N     A+  +       V V+F INLLNYMDR+TI G+L  I+ Y++ ++   +GLLQT F+ SYMV   +FGYLGDR+ RK ++  G+  WS    AS+ + +D +W F+++R ++G+GEASYST+AP II DLFT + RTK+++ F FAIP+GSGLG+++G  +    G WQ  +R TP++GV C+ +  F+V+EP RG           SA  + +S  TS+ +D+K L    SF+++ LGFT V FV GSL+++A  ++ YA K  + +L      +I + FG +T  AG+ GV +GAE  RR R     AD L+CA GLI   P   L++V A      + V +FI + LLC NWA V DM + V+ P RR SA A Q+++ H  GDAGSP+ +G     ++D  R +  + + +  SM+YA+LIN  I V+GG  FI  +FYI  D+           + N  ++D  +L+
Sbjct:   22 TRSPTSGNSSEIMASRFVCGGPNFTVAVLFFINLLNYMDRFTIVGVLSSIQKYFDEEENSVAGLLQTVFVCSYMVFAPIFGYLGDRLRRKYLMALGILVWSGTVFASTLLDQDDYWYFLLLRGVVGIGEASYSTMAPTIIGDLFTGDMRTKMLSIFYFAIPVGSGLGYVVGSKVAHALGSWQWGMRVTPVLGVACVLLCLFVVHEPPRGAIERGFNPNLLSASTVHQS--TSYWDDLKYLFKVKSFIWLDLGFTCVAFVTGSLAFWAPKFLYYAAKMQDLHLRKD---DITFNFGLITCAAGIVGVWLGAELARRWRVYNKRADALICAIGLIGCTPFLYLAIVFAHKSLVSAYVTVFIGEVLLCMNWAPVGDMVLYVIIPPRRSSAGAVQILISHLFGDAGSPWLIG----EVSDLIRGARDTDEGHQTSMEYALLINTFIAVLGGLCFILCAFYIVADREAA-------DRHNHDDDDESLLS 490          
BLAST of Spinster-like protein 1 vs. Ensembl Medaka
Match: SPNS2 (sphingolipid transporter 2 [Source:NCBI gene;Acc:101168642])

HSP 1 Score: 355.525 bits (911), Expect = 5.263e-117
Identity = 202/473 (42.71%), Postives = 287/473 (60.68%), Query Frame = 2
Query:  134 SESDLS--SNGKSFRAAF-KLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA---EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMS 1528
            S  DL   + G+ FR A  K +      VF+ F  N+LNYMDRYT+TG+L DI+ ++ + D   GLLQT FI S+MV   +FGYLGDR NRK+I+ CG+ FWS++TL+SSFI K  +WLFV+ R L+G+GE+SYS+++P II DLFT   RT +++ F  AIP+GSGLG+I+G   K   G W  ALR +P +G+    ++   V EP RGSAD +  +I   TSWI D+K L  N S++F +L    V F  G+   +  IY+  A   +K  +    +  S   ++IFG++T + GL GVVIGA   R  R+    ADPLVCA  ++  A    L  V+AK +   + V IFI +TLL  NWA+  D+ M VV P RR +A AFQ  + H LGDAGSPY +G I  SL   +    +SA   + S+ YA+++   I+V+GG FF+  + +  +D+ K  + + K ++   S
Sbjct:   32 SSPDLHPCTPGQKFRTALLKCRSPIAAAVFLSFG-NVLNYMDRYTVTGVLLDIQRHFQVSDSGIGLLQTVFICSFMVAAPIFGYLGDRFNRKVILSCGIFFWSIVTLSSSFIGKKYYWLFVLSRGLVGIGESSYSSISPTIIGDLFTNNKRTIMLSVFYLAIPLGSGLGYILGAGAKDAAGDWHWALRVSPPLGITAGALILLFVPEPKRGSADQMGGTIMARTSWICDMKALAKNRSYVFSSLASASVSFATGAFGMWIPIYLTRAQVVQKTVDDCTTDVCSSTDSFIFGAITCVTGLLGVVIGAATTRFCRQRTERADPLVCAVSMLGSAIFICLIFVVAKKNIAGAYVCIFIGETLLFVNWAITADILMFVVIPTRRATAVAFQSFISHLLGDAGSPYLIGQISDSLQKSY---TTSALWKFLSLGYALMLCPFIIVLGGMFFLATALFFLDDREKAEKQLNKLTRPPSS 500          
BLAST of Spinster-like protein 1 vs. Ensembl Medaka
Match: spns1 (sphingolipid transporter 1 (putative) [Source:NCBI gene;Acc:101170817])

HSP 1 Score: 348.591 bits (893), Expect = 2.450e-114
Identity = 202/471 (42.89%), Postives = 286/471 (60.72%), Query Frame = 2
Query:  125 KGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGS--ADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYA-----EKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLND------KFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSEN 1498
             GL E    +N +   A+     +  + V V+  INLLNYMDR+T+ G+LP+I+ ++ IDD KSGLLQT FI SYM     FGYLGDR NRK I+  G+ FW+L+TLASSF PK+ FW+ ++ R L+GVGEASYST+AP II+DL+ K+ RT +++ F FAIP+GSGLG+I+G  + +    W  ALR TP +G+V + +L F+V EP RG+  A   ++   TSW+ D++ L  N SF+  T GFT V FV GSL+ +A  ++  A     EKA       SSS ++N  FG +T ++G+ GV  G    RRLR     ADPLVCA GL+L AP   L++V A+     + V IF+ +T L  NWA+V D+ + VV P RR +A A Q+++ H LGDAGSPY +G++   +        +FR    S     +           + V+GG FF+  + +I  D+ + +EN
Sbjct:   21 DGLEEHSRPTNEQEAPASGVSALRALLTVLVLCYINLLNYMDRFTVAGVLPEIENFFGIDDGKSGLLQTVFICSYMFLAPCFGYLGDRYNRKYIMSVGILFWALVTLASSFTPKEHFWVLLLTRGLVGVGEASYSTIAPTIIADLYVKDRRTNMLSCFYFAIPVGSGLGYIVGSQVSSAVKNWHWALRVTPGLGLVAVLLLLFVVQEPKRGAVEARAEEQLHRTSWLTDMRALSRNLSFVLSTFGFTAVAFVTGSLALWAPTFLFRAAVFTGEKAPCSETHCSSSDSLN--FGIITCVSGVLGVASGVAVSRRLRTKTARADPLVCAAGLLLSAPFLYLAIVFAESSTIATYVFIFLGETFLSMNWAIVADILLYVVVPTRRATAEAVQIVLSHLLGDAGSPYLIGVVSDYMRKTDSFLWQFRSLQLSLLLCSF-----------VAVVGGAFFLATALFIEKDRNR-AEN 477          
BLAST of Spinster-like protein 1 vs. Ensembl Medaka
Match: spns3 (protein spinster homolog 3 [Source:NCBI gene;Acc:101168888])

HSP 1 Score: 330.872 bits (847), Expect = 3.334e-107
Identity = 178/446 (39.91%), Postives = 270/446 (60.54%), Query Frame = 2
Query:  188 QKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSI--PTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWF-------AVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADS---NYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKV 1489
             ++ ++ + V+  INLLNYM+RYTI G+L +I+ ++ I D  + LLQT FI S+++   LFGYLGDR NRK I++ G+  W L   ASSF+ +  FWL  ++R L+G+GEASYST+AP II DLFT   R+ ++  F   IP+GSGLG+I G       G W  ALR TPI+GV+ L M+ F+   P RG+A+   + +   +S++EDIK LL N S+++ +LG T + F+ G+L+++       A +  G  +   N+  D++ S    YIFG++TV  G+ G  +G    R  R   PYADPL+C  G++   PC  + + +A      + V IF  + LL  NWA++ D+ + VV P RR +A A Q+   H LGDAGSPY +G+I  +      +S+S  D    +++S++ ++L+   I ++GG FF+F + YIT D   V
Sbjct:   50 NRQAYVTMAVLCYINLLNYMERYTIAGVLLNIQTFFKIRDSTAALLQTVFICSFLLLAPLFGYLGDRYNRKFIMIGGLCVWLLTAAASSFVTESYFWLLALLRGLVGIGEASYSTIAPTIIGDLFTGGKRSIMICIFYIFIPVGSGLGYIAGAGSAYLTGDWHWALRITPIMGVIGLIMMIFLCPNPARGAAETNGEGVVAQSSYLEDIKYLLKNKSYVWSSLGVTALAFLTGALAFWVPTFLSRARVTQGLHQPCTNEPCDSTDS----YIFGAVTVATGILGGAVGTTLSRTFRDKVPYADPLICGVGMLGSVPCIFIIIFVAASSIPATYVFIFCGELLLSLNWAVMADILLYVVVPTRRATAEALQISFAHLLGDAGSPYLIGVISDA------VSSSKPDDLNWSFHSLKVSLLVCPFIGILGGAFFLFTTKYITEDSKAV 485          
BLAST of Spinster-like protein 1 vs. Ensembl Medaka
Match: svopl (SVOP like [Source:NCBI gene;Acc:101157836])

HSP 1 Score: 60.4622 bits (145), Expect = 2.682e-9
Identity = 49/190 (25.79%), Postives = 93/190 (48.95%), Query Frame = 2
Query:  230 NLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVR-----GSADGIKKSI 784
            N++  M+   +  + P+I+  + +DD +  L+ T   L +MVC VL GY+ DR  R  ++  G  + +  +L +SF P   +  F+ +RS++G G A  S     + ++    ++R  L+   +    +GS L  ++G  +    G W+  +R +    +V +F+  FI  E  R     G+ D   K++
Sbjct:   76 NVVEAMEIMLLAVVSPEIRCEWKLDDWQVALVSTIVFLGFMVCGVLSGYMADRFGRWKVVFGGFVWSAYFSLLTSFAPS--YGWFIFLRSMVGCGVAGVSQ-GFVLKTEFIPAKYRAVLLPLATIFWMMGSMLIIVLGMLVVPTLG-WRWMIRISVTPSIVLIFLFKFI-PESARYNVSAGNVDAALKTL 260          
BLAST of Spinster-like protein 1 vs. Ensembl Medaka
Match: svopl (SVOP like [Source:NCBI gene;Acc:101157836])

HSP 1 Score: 60.4622 bits (145), Expect = 2.693e-9
Identity = 49/190 (25.79%), Postives = 93/190 (48.95%), Query Frame = 2
Query:  230 NLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVR-----GSADGIKKSI 784
            N++  M+   +  + P+I+  + +DD +  L+ T   L +MVC VL GY+ DR  R  ++  G  + +  +L +SF P   +  F+ +RS++G G A  S     + ++    ++R  L+   +    +GS L  ++G  +    G W+  +R +    +V +F+  FI  E  R     G+ D   K++
Sbjct:   79 NVVEAMEIMLLAVVSPEIRCEWKLDDWQVALVSTIVFLGFMVCGVLSGYMADRFGRWKVVFGGFVWSAYFSLLTSFAPS--YGWFIFLRSMVGCGVAGVSQ-GFVLKTEFIPAKYRAVLLPLATIFWMMGSMLIIVLGMLVVPTLG-WRWMIRISVTPSIVLIFLFKFI-PESARYNVSAGNVDAALKTL 263          
BLAST of Spinster-like protein 1 vs. Planmine SMEST
Match: SMESG000057652.1 (SMESG000057652.1)

HSP 1 Score: 1001.5 bits (2588), Expect = 0.000e+0
Identity = 495/496 (99.80%), Postives = 495/496 (99.80%), Query Frame = 2
Query:   68 MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM 1555
            MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRR SAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM
Sbjct:    1 MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRASAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM 496          
BLAST of Spinster-like protein 1 vs. Planmine SMEST
Match: SMESG000057652.1 (SMESG000057652.1)

HSP 1 Score: 981.474 bits (2536), Expect = 0.000e+0
Identity = 488/496 (98.39%), Postives = 488/496 (98.39%), Query Frame = 2
Query:   68 MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNINYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM 1555
            MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMN       MTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRR SAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM
Sbjct:    1 MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFVMFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMN-------MTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFLLSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRASAAAFQMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINAPILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM 489          
BLAST of Spinster-like protein 1 vs. Planmine SMEST
Match: SMESG000066785.1 (SMESG000066785.1)

HSP 1 Score: 50.447 bits (119), Expect = 2.547e-6
Identity = 54/176 (30.68%), Postives = 87/176 (49.43%), Query Frame = 2
Query:  317 GLLQTSFILSYMVCCVLFGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGEASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFGVWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQLLINPSFMFI 844
            GLL +SF L   +C  + G L D   RK +++   T   LI+ ++  + ++ F LF + R + GV +A+   VA A+I DL+ +  R   MA    A  +G  +G I+G    T+F V   A+  T +  +  +F+ FF      +   D       +S + DI Q LINP  +F+
Sbjct:   77 GLLGSSFSLCQFLCSPIVGALSDVFGRKSMLILTTT-GMLISYSAWCLSENNFTLFFLSRIIGGVCKANIG-VAIAVIGDLYGETNRNSGMAIIGIAFSLGFIIGPIVGVVAGTFFNV---AMISTGLTLLNLIFISFFFTESLEKSKRD----RNMSSKLSDIYQ-LINPISIFL 242          
The following BLAST results are available for this feature:
BLAST of Spinster-like protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SPNS12.795e-11943.96sphingolipid transporter 1 (putative) [Source:HGNC... [more]
SPNS19.775e-11943.96sphingolipid transporter 1 (putative) [Source:HGNC... [more]
SPNS11.871e-11443.84sphingolipid transporter 1 (putative) [Source:HGNC... [more]
SPNS12.420e-10947.81sphingolipid transporter 1 (putative) [Source:HGNC... [more]
SPNS23.973e-10842.13sphingolipid transporter 2 [Source:HGNC Symbol;Acc... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spin-11.395e-10737.58SPINster (Drosophila lysosomal permease) homolog ... [more]
spin-43.008e-10137.14SPINster (Drosophila lysosomal permease) homolog ... [more]
spin-43.008e-10137.14SPINster (Drosophila lysosomal permease) homolog ... [more]
spin-21.309e-9737.22SPINster (Drosophila lysosomal permease) homolog ... [more]
spin-34.234e-8932.56SPINster (Drosophila lysosomal permease) homolog ... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spin4.112e-11238.96gene:FBgn0086676 transcript:FBtr0089593[more]
spin7.755e-11139.67gene:FBgn0086676 transcript:FBtr0089590[more]
spin1.734e-11038.55gene:FBgn0086676 transcript:FBtr0089591[more]
spin2.652e-10939.26gene:FBgn0086676 transcript:FBtr0089592[more]
spin5.371e-7147.01gene:FBgn0086676 transcript:FBtr0089589[more]
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BLAST of Spinster-like protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spns11.040e-12644.23spinster homolog 1 (Drosophila) [Source:NCBI gene;... [more]
spns11.040e-12644.23spinster homolog 1 (Drosophila) [Source:NCBI gene;... [more]
spns13.194e-12644.87spinster homolog 1 (Drosophila) [Source:NCBI gene;... [more]
spns23.739e-11341.05spinster homolog 2 (Drosophila) [Source:NCBI gene;... [more]
spns22.138e-11241.37spinster homolog 2 (Drosophila) [Source:NCBI gene;... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spns24.024e-11141.23spinster homolog 2 [Source:NCBI gene;Acc:100145187... [more]
spns21.195e-11041.19spinster homolog 2 [Source:NCBI gene;Acc:100145187... [more]
spns21.455e-11041.19spinster homolog 2 [Source:NCBI gene;Acc:100145187... [more]
cpne51.117e-10044.33copine V [Source:Xenbase;Acc:XB-GENE-956274][more]
spns24.188e-9738.68spinster homolog 2 [Source:NCBI gene;Acc:100145187... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Spns19.020e-12044.15spinster homolog 1 [Source:MGI Symbol;Acc:MGI:1920... [more]
Spns12.914e-11347.80spinster homolog 1 [Source:MGI Symbol;Acc:MGI:1920... [more]
Spns23.972e-10842.36spinster homolog 2 [Source:MGI Symbol;Acc:MGI:2384... [more]
Spns14.053e-10440.98spinster homolog 1 [Source:MGI Symbol;Acc:MGI:1920... [more]
Spns31.991e-9838.65spinster homolog 3 [Source:MGI Symbol;Acc:MGI:1924... [more]
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BLAST of Spinster-like protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7ZU13|SPNS1_DANRE2.411e-12544.87Protein spinster homolog 1 OS=Danio rerio OX=7955 ... [more]
sp|Q5XGK0|SPNS1_XENLA1.349e-12446.05Protein spinster homolog 1 OS=Xenopus laevis OX=83... [more]
sp|A8WGF7|SPNS1_XENTR2.501e-12143.56Protein spinster homolog 1 OS=Xenopus tropicalis O... [more]
sp|Q2YDU8|SPNS1_RAT3.779e-12044.37Protein spinster homolog 1 OS=Rattus norvegicus OX... [more]
sp|Q8R0G7|SPNS1_MOUSE6.309e-11944.15Protein spinster homolog 1 OS=Mus musculus OX=1009... [more]
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BLAST of Spinster-like protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3JSB82.578e-13644.25protein spinster homolog 1 isoform X2 OS=Lingula u... [more]
K1QTC72.792e-13645.57Spinster-like protein 1 OS=Crassostrea gigas OX=29... [more]
A0A2R2MTX21.590e-13544.82protein spinster homolog 1 isoform X1 OS=Lingula u... [more]
A0A3P8VVS14.484e-13244.70Spinster homolog 1 (Drosophila) OS=Cynoglossus sem... [more]
T1J6E41.239e-13045.00MFS domain-containing protein OS=Strigamia maritim... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
spns16.545e-12345.23sphingolipid transporter 1 (putative) [Source:NCBI... [more]
spns11.025e-12245.23sphingolipid transporter 1 (putative) [Source:NCBI... [more]
SPNS25.712e-10942.82sphingolipid transporter 2 [Source:NCBI gene;Acc:1... [more]
spns32.677e-9842.38spinster homolog 3 (Drosophila) [Source:NCBI gene;... [more]
SPNS24.360e-9841.31sphingolipid transporter 2 [Source:NCBI gene;Acc:1... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000008246.14.915e-11241.31pep scaffold:Pmarinus_7.0:GL480278:6713:20715:1 ge... [more]
ENSPMAT00000006804.18.792e-7440.64pep scaffold:Pmarinus_7.0:GL479580:1268:6755:1 gen... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
HXT25.008e-623.20High-affinity glucose transporter of the major fac... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO394331.062e-11640.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Spinster-like protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SPNS25.263e-11742.71sphingolipid transporter 2 [Source:NCBI gene;Acc:1... [more]
spns12.450e-11442.89sphingolipid transporter 1 (putative) [Source:NCBI... [more]
spns33.334e-10739.91protein spinster homolog 3 [Source:NCBI gene;Acc:1... [more]
svopl2.682e-925.79SVOP like [Source:NCBI gene;Acc:101157836][more]
svopl2.693e-925.79SVOP like [Source:NCBI gene;Acc:101157836][more]
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BLAST of Spinster-like protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000057652.10.000e+099.80SMESG000057652.1[more]
SMESG000057652.10.000e+098.39SMESG000057652.1[more]
SMESG000066785.12.547e-630.68SMESG000066785.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015055 ID=SMED30015055|Name=Spinster-like protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1594bp
AATTTATTTTCACAATTGAAACCCACTTGAAATCATGAGCACATTAATTA
TATGTTTTTTTTAATTTATGCGTGAAAGATCTAGTTTAACGTGGAATAAT
TCTTTGGTCCTCTCACGGTCATGGAAAGGTTTGTCAGAAAGTGATTTGTC
ATCCAATGGAAAATCTTTTCGTGCTGCTTTCAAATTACAGAAGAAAGACT
TCATCGTTGTGTTTGTCATGTTTATTATTAATCTACTGAATTATATGGAC
AGATATACAATAACTGGTCTACTTCCTGATATCAAGGGTTATTATAACAT
AGATGACGAAAAGTCTGGACTGTTGCAAACGTCTTTTATTTTGAGCTATA
TGGTTTGTTGTGTGCTATTCGGTTATCTTGGGGATAGAATCAATAGGAAG
CTGATTATAATGTGTGGGGTAACCTTTTGGTCATTAATTACTTTAGCAAG
CTCCTTCATTCCTAAAGATATGTTTTGGTTGTTTGTTGTTATGCGTTCAT
TGCTTGGTGTCGGAGAAGCTAGTTACAGCACAGTTGCTCCAGCTATTATT
TCAGATCTCTTTACAAAAGAATGGCGGACTAAATTAATGGCGTTTTTTTC
ATTTGCTATTCCTATTGGAAGTGGTTTGGGTTTCATAATTGGATTTGCTT
TAAAAACTTGGTTTGGGGTGTGGCAAGCAGCGTTGCGTTTTACTCCAATA
ATTGGAGTTGTATGTTTGTTCATGTTATTCTTTATTGTAAATGAACCAGT
GAGGGGTAGTGCAGACGGAATCAAAAAGTCCATACCAACATCATGGATCG
AAGATATTAAACAGTTGCTTATCAATCCTAGTTTTATGTTTATTACACTT
GGATTTACAGTTGTATGCTTTGTTCTTGGATCTTTATCGTGGTTTGCTGT
AATATATATGGGATATGCTGAGAAAGCATATAATCAGTATTTGGATAATT
CTAGTTCTATGAATATAAACTATATTTTCGGAAGTATGACTGTCTTAGCG
GGACTGTTTGGAGTAGTTATTGGAGCAGAAGCTGGAAGGCGTTTGCGAAG
AATATTCCCGTATGCTGATCCATTAGTTTGTGCATTCGGTCTAATTTTAG
GAGCTCCGTGCTTTTTACTTAGCATGGTACTTGCTAAATATCATTTTAAT
TGGTCTTTGGTTGTTATATTCATTGCTCAAACTCTACTTTGCTTCAACTG
GGCATTAGTAACGGATATGACAATGAGTGTTGTCAATCCATGGCGACGAG
GTTCAGCAGCAGCCTTCCAAATGATAGTAGGTCATGCCCTAGGTGATGCT
GGGAGTCCATATTATGTTGGAATAATCAAAAGTTCTTTAAATGATAAATT
CCGAATCTCTGCCAGTTCAGCAGACTCAAATTATTACAGCATGCAATACG
CCATGTTAATTAATGCTCCGATTTTAGTTATTGGAGGTTTCTTTTTCATA
TTTGCTTCCTTCTATATCACAAATGACAAATTCAAAGTCTCGGAAAACGT
TATAAAGTATTCAAAAAGCAATATGTCAAATGAAGATGCACGAATTTTAA
CAATGTAATGATTATGAATCTATCTAATAAATTTTGAATTTACT
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protein sequence of SMED30015055-orf-1

>SMED30015055-orf-1 ID=SMED30015055-orf-1|Name=SMED30015055-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=497bp
MRERSSLTWNNSLVLSRSWKGLSESDLSSNGKSFRAAFKLQKKDFIVVFV
MFIINLLNYMDRYTITGLLPDIKGYYNIDDEKSGLLQTSFILSYMVCCVL
FGYLGDRINRKLIIMCGVTFWSLITLASSFIPKDMFWLFVVMRSLLGVGE
ASYSTVAPAIISDLFTKEWRTKLMAFFSFAIPIGSGLGFIIGFALKTWFG
VWQAALRFTPIIGVVCLFMLFFIVNEPVRGSADGIKKSIPTSWIEDIKQL
LINPSFMFITLGFTVVCFVLGSLSWFAVIYMGYAEKAYNQYLDNSSSMNI
NYIFGSMTVLAGLFGVVIGAEAGRRLRRIFPYADPLVCAFGLILGAPCFL
LSMVLAKYHFNWSLVVIFIAQTLLCFNWALVTDMTMSVVNPWRRGSAAAF
QMIVGHALGDAGSPYYVGIIKSSLNDKFRISASSADSNYYSMQYAMLINA
PILVIGGFFFIFASFYITNDKFKVSENVIKYSKSNMSNEDARILTM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 37..231
e-value: 3.4E-41
score: 142.9
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 246..470
e-value: 2.0E-12
score: 48.8
NoneNo IPR availablePANTHERPTHR23505FAMILY NOT NAMEDcoord: 36..485
NoneNo IPR availablePANTHERPTHR23505:SF3PROTEIN SPINSTER HOMOLOG 3coord: 36..485
NoneNo IPR availableCDDcd17328MFS_spinster_likecoord: 48..461
e-value: 5.02562E-150
score: 432.43
NoneNo IPR availableTMHMMTMhelixcoord: 202..224
NoneNo IPR availableTMHMMTMhelixcoord: 45..63
NoneNo IPR availableTMHMMTMhelixcoord: 83..105
NoneNo IPR availableTMHMMTMhelixcoord: 256..278
NoneNo IPR availableTMHMMTMhelixcoord: 445..467
NoneNo IPR availableTMHMMTMhelixcoord: 397..419
NoneNo IPR availableTMHMMTMhelixcoord: 333..355
NoneNo IPR availableTMHMMTMhelixcoord: 360..382
NoneNo IPR availableTMHMMTMhelixcoord: 173..195
NoneNo IPR availableTMHMMTMhelixcoord: 112..131
NoneNo IPR availableTMHMMTMhelixcoord: 299..318
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 52..406
e-value: 1.3E-39
score: 136.2
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 47..468
score: 27.69
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 37..463