SMED30015004

Overview
NameSMED30015004
Smed IDSMED30015004
Length (bp)2145
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30015004 (SMED30015004) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30015004 (SMED30015004) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30015004 (SMED30015004) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30015004 (SMED30015004) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30015004

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30015004h1SMcG0015749 SmedASXL_015288SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30015004h1SMcG0015749 Contig48405newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30015004h1SMcG0015749 Contig48405uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30015004h1SMcG0015749 dd_Smed_v4_13840_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30015004h1SMcG0015749 dd_Smed_v4_13840_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30015004h1SMcG0015749 dd_Smed_v4_13840_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30015004h1SMcG0015749 dd_Smed_v6_13840_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30015004 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000650:90313..97287 -10620
Homology
[Close]
BLAST of SMED30015004 vs. TrEMBL
Match: A0A267FF45 (Death domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016879g1 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 3.421e-26
Identity = 127/561 (22.64%), Postives = 266/561 (47.42%), Query Frame = 2 
Query:  326 SHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFEKFQINR--FEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVAS-------VRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLE------QKTIKSELSIKQRQTKIINLE---KQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTK---FQIEDNQAVMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKEL 1945
            SHR  K    ++  V +AA  ++  L++++E + +K++      +Q   + +++ F++E  K+I        +F         P     + + L    D  F+ +++ + +  +   +++ GL+KYC+ L + C ++ID V  YEK   + + ++ K++ D+         FT ++++   +  + + S       +++IRA+INK+YY V EWVD +R Y  +++D  +  +  L  L+      +  ++ E  +K+ Q +I  LE   KQLKI +  +S           SQ+  +++L  +E E   +     +++ +   LE  +Q++  Q   R    +   ++     Q+R+      R +D   ++ R+ + L          +++T     ++  NQ  M +  E  QE +     + E    L+ +  ++N + + K S  +L++L   G +++  K        C  ++ ++  DW++LY +LPM P+R+   R  D+E +D+I  R D    +     L++WR+    + PE L++ L+K    E+A+ +
Sbjct:   41 SHRGGKGPPDSLDSVQDAAVSVIGTLNSRVETLHDKYKWEEPLTKQTQQLQQLTAFIAE--KRIEGRFRTFKEFNALFDASSLPQDLRPLYDQLGALYDS-FNSKVIRNAVF-RLTESLVNGLVKYCLPLGELCATEIDIVRQYEKTHARLDSELNKDLHDL---------FTTTNKMEKLNFVKFLKSPEKSNDFLKKIRAAINKMYYIVNEWVDCDRTYIEDITDRLQEGRQRLEELDSEIEKARSNLQDERQMKKTQDEIDKLEEKAKQLKIKQNKYSA----------SQQSNTTRLSSLEAELQEKTKRKSKLEEQRSKLE--EQQRSGQGTSR---SRTQQDLEWVTLQEREAGQDLERHKDTIASVQRYQKHLESSRAANDTDLRSTNEKLAKLRSNQDSMRRRHEKAQEQLAKLLAEQE---ALEPQVAELNLLRSKKLSSGSLKELHASGHINSPRKVQKPIAVACGDVAQQIGADWRNLYAHLPMEPDRTRSTRRRDVEKLDIIGNRRDLPEPELAAQSLERWRMMNPQSKPEGLLQALRKIERDELAQRV 570
[Close]
BLAST of SMED30015004 vs. TrEMBL
Match: A0A267ERT6 (Death domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016879g2 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 4.250e-26
Identity = 132/566 (23.32%), Postives = 267/566 (47.17%), Query Frame = 2 
Query:  326 SHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFEKFQINR--FEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFH--GISN---KFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVAS-------VRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLE------QKTIKSELSIKQRQTKIINLE---KQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTK---FQIEDNQAVMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKEL 1945
            SHR  K    ++  V +AA  ++  L++++E + +K++      +Q   + +++ F++E  K+I        +F         P      Q+L  +Y D + +   LF+   I N   +   +++ GL+KYC+ L + C ++ID V  YEK   + + ++ K++ D+         FT  +++   +  + + S       +++IRA+INK+YY V EWVD +R Y  +++D  +  +  L  L+      +  ++ E  +K+ Q +I  LE   KQLKI +  +S           SQ+  +++L  +E E   +     +++ +   LE  +Q++  Q   R    +   ++     Q+R+      R +D   ++ R+ + L          +++T     ++  NQ  M +  E  QE +     + E    L+ +  ++N + + K S  +L++L   G +++  K        C  ++ ++  DW++LY +LPM P+R+   R  D+E +D+I  R D    +     L++WR+    + PE L++ L+K    E+A+ +
Sbjct:   41 SHRGGKGPPDSLDSVQDAAVSVIGTLNSRVETLHDKYKWEEPLTKQTQQLQQLTAFIAE--KRIEGRFRTFKEFNALFDASSLP------QDLRPLY-DQLGALYDLFNSKAIRNAVFRLTESLVNGLVKYCLPLGELCATEIDIVRQYEKTHARLDSELNKDLHDL---------FTTINKMEKLNFVKFLKSPEKSNDFLKKIRAAINKMYYIVNEWVDCDRTYIEDITDRLQEGRQRLEELDSEIEKARSNLQDERQMKKTQDEIDKLEEKAKQLKIKQNKYSA----------SQQSNTTRLSSLEAELQEKTKRKSKLEEQRSKLE--EQQRSGQGTSR---SRTQQDLEWVTLQEREAGQDLERHKDTIASVQRYQKHLESSRAANDTDLRSTNEKLAKLRSNQDSMRRRHEKAQEQLAKLLAEQE---ALETQVAELNLLRSKKLSSGSLKELHASGHINSPRKVQKPIAVACGDVAQQIGADWRNLYAHLPMEPDRTRSTRRRDVEKLDIIGNRRDLPEPELAAQSLERWRMMNPQSKPEGLLQALRKIERDELAQRV 570
[Close]
BLAST of SMED30015004 vs. TrEMBL
Match: A0A1I8G8F1 (Death domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 3.526e-25
Identity = 128/562 (22.78%), Postives = 267/562 (47.51%), Query Frame = 2 
Query:  326 SHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFEKFQINR--FEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVAS-------VRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLE------QKTIKSELSIKQRQTKIINLE---KQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTK---FQIEDNQAVMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKELK 1948
            SHR  K    ++  V +AA  ++  L++++E + +K++      +Q   + +++ F++E  K+I        +F         P     + + L    D  F+ +++ + +  +   +++ GL+KYC+ L + C ++ID V  YEK   + + ++ K++ D+         FT ++++   +  + + S       +++IRA+INK+YY V EWVD +R Y  +++D  +  +  L  L+      +  ++ E  +K+ Q +I  LE   KQLKI          K N+   SQ+  +++L  +E E   +     +++ +   LE  +Q++  Q   R    +   ++     Q+R+      R +D   ++ R+ + L          +++T     ++  NQ  M +  E  QE +     + E    L+ +  ++N + + K S  +L++L   G +++  K        C  ++ ++  DW++LY +LPM P+R+   R  D+E +D+I  R D    +     L++WR+    + PE L++ L+K    E+A+ ++
Sbjct:   75 SHRGGKGPPDSLDSVQDAAVSVIGTLNSRVETLHDKYKWEEPLTKQTQQLQQLTAFIAE--KRIEGRFRTFKEFNALFDASSLPQDLRPLYDQLGALYDS-FNSKVIRNAVF-RLTESLVNGLVKYCLPLGELCATEIDIVRQYEKTHARLDSELNKDLHDL---------FTTTNKMEKLNFVKFLKSPEKSNDFLKKIRAAINKMYYIVNEWVDCDRTYIEDITDRLQEGRQRLEELDSEIEKARSNLQDERQMKKTQDEIDKLEEKAKQLKI----------KQNKYSASQQSNTTRLSSLEAELQEKTKRKSKLEEQRSKLE--EQQRSGQGTSR---SRTQQDLEWVTLQEREAGQDLERHKDTIASVQRYQKHLESSRAANDTDLRSTNEKLAKLRSNQDSMRRRHEKAQEQLAKLLAEQE---ALEPQVAELNLLRSKKLSSGSLKELHASGHINSPRKVQKPIAVACGDVAQQIGADWRNLYAHLPMEPDRTRSTRRRDVEKLDIIGNRRDLPEPELAAQSLERWRMMNPQSKPEGLLQALRKIERDELAQRVE 605
[Close]
BLAST of SMED30015004 vs. TrEMBL
Match: K1PG20 (Death domain-containing protein OS=Crassostrea gigas OX=29159 GN=CGI_10005887 PE=4 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 6.636e-16
Identity = 116/515 (22.52%), Postives = 220/515 (42.72%), Query Frame = 2 
Query:  461 LSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQI------LFHGISNKFQHTVIGGLLKYCVSLAK--YCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQ-------NKQFKKFTNSSRLIVYHVTELVASVRQIRASINKLYYFVTEWVDEERNYPLNLSD--LFKTIKSELFTLEQKTIKSELSIKQRQTKIINLEKQLKI---YEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTKFQIEDNQAVMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKEL 1945
            L  D  +  L+E+     +D+   S  K+        L+  DI++  + Y + +F C+       +   +  +        L KYC S  +  Y    I  +I YE+LI  ++ +I +++  + N           F +F +   +    + E+     +I  +I K       W+ ++ +YP  L     F     E      K ++ E     +Q  + NL+K+ ++     ++ + +K++ ++++NS E ++ K++++E +  N+  E+ ++K  V                + P      + L  K ++ +  A   R  + K  + R   RL K  + + D+    K ++  N+    +M++ +       +   E L  +  K + +  +   K SPDTLR++    +   E       D  C  ++  +  DWK LY  LP  P R   +R  D+E+ID ISAR D TP++     L+KWR F        LI  L+K N  E+A++L
Sbjct:   49 LKYDSCATCLNELLASWQMDSSQFSTSKEFWEKHKIKLVDEDIRKKKE-YKELVFICERARTFERMIPNLRERLVECARDHLYKYCRSFIEETYDEVQIRPIIEYEELITTSKKEIDQKIDSLNNNILKYGEMKSPFSEFISKGHIHTALMEEICVLNIEIAHAIKK-------WIADDSSYPERLLQEVFFNNSYKENLVENIKKLEEE-----KQVVVKNLDKKHRVNYAVMRDHAYHKKEKHKLKNSLETVNLKIEKLEKQIENKNEEISELKEAVAD--------------KTPMAPRDRQELRRKLEKAE--ADLDRFHERKDVMERQHGRLDKELKQVSDRTYELKVEVVTNRHDQEEMRQGILGIEIEMKSILERLSSIDEKQEILKRVRELKLSPDTLRRINTRRQ---EVTTKVQLDDACRYVAFHIGKDWKKLYDRLPFVPPRDPDRRQKDVEVIDNISARQDRTPEESALRSLEKWRSFNRQGDIIQLIRGLRKLNKVELAQKL 531
[Close]
BLAST of SMED30015004 vs. TrEMBL
Match: A0A0R3W2V6 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS4195 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 9.936e-15
Identity = 125/569 (21.97%), Postives = 242/569 (42.53%), Query Frame = 2 
Query:  281 MSSRSHTKSLDRDIQS------HRKPKAYYLNIQQVNNAAEHLVCHLHNKIEN-----IFEKFQINRFEQELSIDKISEFLSEI---SKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVASVRQIRASINKLYYFVTEWVDEERNYPLNLSDLFK----TIKSELFTLE-------QKTIKSELSIKQRQTKIINLEKQLKIYEKNFSIYKR--KLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSL----EHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTKFQIEDNQAVMVKMKENLQEDINSQREDNENLKILQIK--SKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDI------EIIDLISARNDSTPKKQTRMCLDKWRLF 1870
            M+ RS  +S +R  Q       ++  + +  +IQ+  +A       LH   E+     + E+  IN F+ +L++D +S+   ++     +++ID  V  +  + L          D++ L +   DF       F  ++ +    +  G+  +C SL +    +++ VI Y   I K   ++ + V  I+   + +   T  ++  + H+ EL A +  IR   NK+     EW++++R YP  L++  K    T++  L  +E       QKT +     + R+ ++  L+K+ +    N  + +R  + N         ++KL   + ++    G+ ++  S   S      +   + KPQK++    Y+                              R   +L K    ++  ++    +I D Q      ++  + D N +R      +I QIK  S D   IL  +    TL QL+  G ++   +G    +++C  ++ +V H+W  LY++LPM+P RS+ QR  DI      E ++++  RN  T  +     L+KWRL 
Sbjct:    1 MAQRSRNRSQNRTRQPQSLESLYKTTQNFCNSIQKTLDAVGQQCISLHIDKEDQYVMKVLER--INAFDDDLNMDALSDAQCQLLIEDLELDIDEGVALEIVKGL---------RDLELLRKSAADF-------FSTLNKRLSSMLDNGVYVFCASLIELTQENVNEVIEYNDFISKQRSELTQAVDQIKQYQEIWSNLTMDTQSTLNHLKELGACLEGIREEWNKIRAKTIEWINQDREYPTRLANRIKEHRETMQGLLLEIEAISETKQQKTGER----RHRELRMEALQKRRRELCSNLRLVQRAKRFN---------ATKLAAFDSDSAQTEGDREECNSAKPSTTSDGGNAINDSKPQKEL----YR------------------------------RRDTKLEKEQNDIKSSLKNVNKRITDLQRAA--GEDERESDTNERRLSEIETEIAQIKKISTDCREILLQRGKVQTLLQLQ-RGVVAETSRGKIPFERICKFVALRVRHEWPSLYLHLPMWPTRSLSQRQDDITTLLGFERMNVVLPRN-RTSVEDAAAALEKWRLL 500
[Close]
BLAST of SMED30015004 vs. Planmine SMEST
Match: SMESG000042350.1 (SMESG000042350.1)

HSP 1 Score: 1165.98 bits (3015), Expect = 0.000e+0
Identity = 577/579 (99.65%), Postives = 578/579 (99.83%), Query Frame = 2 
Query:  281 MSSRSHTKSLDRDIQSHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFEKFQINRFEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVASVRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLEQKTIKSELSIKQRQTKIINLEKQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTKFQIEDNQAVMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKELKDESLKTKNKFEDDEAIVDNPLIL 2017
            MSSRSHTKSLDRDIQSHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFEKFQINRFEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVASVRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLEQKT+KSELSIKQRQTKIINLEKQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTKFQIEDNQA MVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKELKDESLKTKNKFEDDEAIVDNPLIL
Sbjct:    1 MSSRSHTKSLDRDIQSHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFEKFQINRFEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLISSDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSDIDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELVASVRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLEQKTVKSELSIKQRQTKIINLEKQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKEIEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATKTQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTKFQIEDNQAAMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLRNHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHHDIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCEIAKELKDESLKTKNKFEDDEAIVDNPLIL 579
The following BLAST results are available for this feature:
BLAST of SMED30015004 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267FF453.421e-2622.64Death domain-containing protein OS=Macrostomum lig... [more]
A0A267ERT64.250e-2623.32Death domain-containing protein OS=Macrostomum lig... [more]
A0A1I8G8F13.526e-2522.78Death domain-containing protein OS=Macrostomum lig... [more]
K1PG206.636e-1622.52Death domain-containing protein OS=Crassostrea gig... [more]
A0A0R3W2V69.936e-1521.97Uncharacterized protein OS=Taenia asiatica OX=6051... [more]
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BLAST of SMED30015004 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30015004 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000042350.10.000e+099.65SMESG000042350.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30015004 ID=SMED30015004|Name=SMED30015004|organism=Schmidtea mediterranea sexual|type=transcript|length=2145bp
TTCCGATCTCAATAATTGTATGTTAATTGTTTTAATTGTACACTTGTGAT
GTATGCGACAAATCCTGTGCAGGTGTGCTTGCATTATTGTCAATATTTTC
TCGCTTATTTTGGAATTTTTGATACAAATTTAATCTATATATCTTCGCGA
TTCGATAATTTGTCTACCAAATTCTCTATTCCATTAACTTTAGTGGTTAT
CTAATATTTCTGTTTCGCTGCCAAGGTGATTAAAACAAATTCATTTATGT
AATTCGCCTTGAAAATTTATCGCCATTTCTATGAGTTCTCGAAGTCACAC
AAAATCATTGGATCGGGATATTCAGTCTCATCGAAAACCGAAGGCATATT
ATCTCAATATTCAACAGGTTAATAACGCAGCCGAACATTTGGTTTGCCAT
CTTCATAATAAAATCGAAAATATTTTTGAAAAATTCCAGATAAATCGATT
TGAACAAGAATTGTCAATCGATAAGATTTCAGAATTCCTCTCAGAGATTT
CCAAAAAAATCAATATCGATGCCATAGTATTGTCGAAATTTAAACAATCT
CTCGTGACGAAATTTTTTCCGTTAATTTCTTCTGATATTCAGGAATTATT
GCAAATGTATTTAGATTTTATATTCAGCTGTCAGATTCTTTTTCATGGAA
TATCTAACAAATTCCAACATACGGTTATCGGAGGTTTGTTAAAATATTGC
GTCAGTCTCGCGAAATATTGCAATTCTGATATAGATTTTGTAATCAACTA
TGAAAAACTCATTCAAAAAAACGAGCCTGACATCATTAAAGAAGTGAAGG
ATATAGAAAATCAAAACAAACAATTCAAAAAGTTTACAAATTCCTCTCGT
TTGATTGTTTATCACGTAACCGAGTTAGTCGCCTCAGTACGGCAGATTCG
GGCATCGATAAATAAACTTTATTATTTTGTCACAGAATGGGTCGATGAAG
AAAGAAATTATCCCCTGAATTTGTCAGATCTATTTAAAACAATAAAATCA
GAATTATTTACTTTAGAACAGAAAACCATCAAAAGCGAGCTTTCGATCAA
GCAAAGACAAACAAAAATTATAAATTTAGAGAAACAATTGAAAATCTACG
AAAAAAACTTTTCAATTTATAAACGAAAACTGAATCGTATTAGAAATAGT
CAAGAGCAAATATCAAGCAAGTTAAAAGAAATTGAAATTGAAACAATAAA
TCAAGCTGGTGAATTAAAACAAATAAAGAGTAAAGTTAAATCTCTTGAAC
ATTTAAAACAAGAGAAAAAACCACAAAAGAAAATTAGAATTCCTCATTAC
AAAGTCTCCATGGAAATTCTGGCAACGAAAACCCAACAAAGGGATTTTGT
GGCGAAAAATATGAGATTAGAAGACAATAAACATGCTCTGAGTCGGCACA
GTGAGAGATTGTCAAAAATTGCCGAGTTGTTACGGGATAAAATTCAAACA
ACAAAATTCCAAATTGAAGACAATCAAGCTGTAATGGTGAAAATGAAAGA
AAATTTACAAGAGGATATAAATTCCCAGAGAGAAGACAATGAGAATTTAA
AGATTTTACAGATTAAGTCAAAAGATATCAATTTCATTTTGACAACAAAA
AATTCTCCTGACACTTTAAGGCAGCTTCGTAACCACGGGAAATTGAGCAA
TGAATATAAAGGAATCAACCTTACAGACAAACTATGTGACATGCTATCAA
CAAAAGTAATTCATGACTGGAAGGACCTTTATATAAATCTTCCAATGTTT
CCCGAAAGATCTATTCAACAAAGACATCATGACATTGAAATAATTGACTT
AATCAGTGCTAGAAATGATAGCACTCCTAAAAAGCAAACGAGAATGTGTT
TAGATAAGTGGAGATTATTCTATCACAATGCCACTCCCGAGCATTTGATC
GAAACACTTCAGAAAACAAACAACTGTGAAATCGCCAAAGAATTGAAAGA
TGAAAGTTTAAAAACTAAAAATAAATTTGAAGATGATGAAGCAATTGTTG
ATAATCCTCTTATACTATAATTATTTACCGCGAGATTAATTTACCACTTT
ATTATGATATTTAGTCCATTTTTAAAACTATTTTTAATATTTCTTATATC
TTTATAAATATAATTTTTATTTAAAGTTTTTCTTCATATCCAAAC
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protein sequence of SMED30015004-orf-1

>SMED30015004-orf-1 ID=SMED30015004-orf-1|Name=SMED30015004-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=580bp
MSSRSHTKSLDRDIQSHRKPKAYYLNIQQVNNAAEHLVCHLHNKIENIFE
KFQINRFEQELSIDKISEFLSEISKKINIDAIVLSKFKQSLVTKFFPLIS
SDIQELLQMYLDFIFSCQILFHGISNKFQHTVIGGLLKYCVSLAKYCNSD
IDFVINYEKLIQKNEPDIIKEVKDIENQNKQFKKFTNSSRLIVYHVTELV
ASVRQIRASINKLYYFVTEWVDEERNYPLNLSDLFKTIKSELFTLEQKTI
KSELSIKQRQTKIINLEKQLKIYEKNFSIYKRKLNRIRNSQEQISSKLKE
IEIETINQAGELKQIKSKVKSLEHLKQEKKPQKKIRIPHYKVSMEILATK
TQQRDFVAKNMRLEDNKHALSRHSERLSKIAELLRDKIQTTKFQIEDNQA
VMVKMKENLQEDINSQREDNENLKILQIKSKDINFILTTKNSPDTLRQLR
NHGKLSNEYKGINLTDKLCDMLSTKVIHDWKDLYINLPMFPERSIQQRHH
DIEIIDLISARNDSTPKKQTRMCLDKWRLFYHNATPEHLIETLQKTNNCE
IAKELKDESLKTKNKFEDDEAIVDNPLIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR011029DEATH-like_dom_sf
IPR000488Death_domain
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 406..426
NoneNo IPR availableCOILSCoilCoilcoord: 256..304
NoneNo IPR availableGENE3DG3DSA:1.10.533.10coord: 464..567
e-value: 2.4E-10
score: 42.1
NoneNo IPR availableCDDcd01670Deathcoord: 472..556
e-value: 4.40275E-5
score: 39.9577
IPR000488Death domainPROSITEPS50017DEATH_DOMAINcoord: 505..559
score: 10.794