SMED30014946
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014946 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30014946 aligns in the following genomic locations:
Homology
BLAST of SMED30014946 vs. Planmine SMEST
Match: SMESG000013600.1 (SMESG000013600.1) HSP 1 Score: 524.242 bits (1349), Expect = 0.000e+0 Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 1 Query: 40 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQNDTRLSSMS 825 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQNDTRLSSMS Sbjct: 1 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQNDTRLSSMS 262 HSP 2 Score: 72.7886 bits (177), Expect = 1.744e-26 Identity = 30/33 (90.91%), Postives = 32/33 (96.97%), Query Frame = 2 Query: 1265 DFFVGNFIFPNPYLDKLWFCQKSHSGLFLKLVS 1363 DFFVGNFIFPNPYLDKLW CQKSHSGLFLKL++ Sbjct: 263 DFFVGNFIFPNPYLDKLWLCQKSHSGLFLKLIT 295 HSP 3 Score: 66.2402 bits (160), Expect = 1.744e-26 Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 3 Query: 1455 PVTDRFPLPIPWNCRHQRTRTIAVGCRL 1538 PVTDRFPLPIPWNCRHQRTRTIAVGCRL Sbjct: 306 PVTDRFPLPIPWNCRHQRTRTIAVGCRL 333
BLAST of SMED30014946 vs. Planmine SMEST
Match: SMESG000013600.1 (SMESG000013600.1) HSP 1 Score: 510.76 bits (1314), Expect = 0.000e+0 Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 1 Query: 40 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQND 804 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQND Sbjct: 1 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQND 255 HSP 2 Score: 66.2402 bits (160), Expect = 3.187e-17 Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 3 Query: 1455 PVTDRFPLPIPWNCRHQRTRTIAVGCRL 1538 PVTDRFPLPIPWNCRHQRTRTIAVGCRL Sbjct: 284 PVTDRFPLPIPWNCRHQRTRTIAVGCRL 311 HSP 3 Score: 41.5874 bits (96), Expect = 3.187e-17 Identity = 16/19 (84.21%), Postives = 18/19 (94.74%), Query Frame = 2 Query: 1307 DKLWFCQKSHSGLFLKLVS 1363 DKLW CQKSHSGLFLKL++ Sbjct: 255 DKLWLCQKSHSGLFLKLIT 273
BLAST of SMED30014946 vs. Planmine SMEST
Match: SMESG000013600.1 (SMESG000013600.1) HSP 1 Score: 421.779 bits (1083), Expect = 2.449e-147 Identity = 212/214 (99.07%), Postives = 214/214 (100.00%), Query Frame = 1 Query: 40 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMF 681 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSM++ Sbjct: 1 MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMIY 214
BLAST of SMED30014946 vs. Planmine SMEST
Match: SMESG000013600.1 (SMESG000013600.1) HSP 1 Score: 254.988 bits (650), Expect = 2.472e-82 Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 1 Query: 457 MFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQNDTRLSSMS 825 MFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQNDTRLSSMS Sbjct: 1 MFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTINFHNDLDIDPVVILEICDAQYKEYSLWIKLQNDTRLSSMS 123 HSP 2 Score: 71.633 bits (174), Expect = 1.020e-25 Identity = 30/33 (90.91%), Postives = 32/33 (96.97%), Query Frame = 2 Query: 1265 DFFVGNFIFPNPYLDKLWFCQKSHSGLFLKLVS 1363 DFFVGNFIFPNPYLDKLW CQKSHSGLFLKL++ Sbjct: 124 DFFVGNFIFPNPYLDKLWLCQKSHSGLFLKLIT 156 HSP 3 Score: 64.6994 bits (156), Expect = 1.020e-25 Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 3 Query: 1455 PVTDRFPLPIPWNCRHQRTRTIAVGCRL 1538 PVTDRFPLPIPWNCRHQRTRTIAVGCRL Sbjct: 167 PVTDRFPLPIPWNCRHQRTRTIAVGCRL 194
BLAST of SMED30014946 vs. Planmine SMEST
Match: SMESG000038450.1 (SMESG000038450.1) HSP 1 Score: 112.464 bits (280), Expect = 2.424e-26 Identity = 67/243 (27.57%), Postives = 132/243 (54.32%), Query Frame = 1 Query: 85 QNEVVYSLCSNFLCKNI-IINIRKESVAVEEYVYNIITYGSLTPQLRICTSIIIYAPQLFKYSEKSIVSEISSAYLKSTKQFITLYLFKDVIQKLRTELSLSKNSEDYFFQNIFNQLLLRLDIYDMFLVEYLSTFKCIKCNKSFTKSVKGDAILFPYIPQAFPKFTKNKMYYPVICLKCNESVNVELEFLKSGNVLSMMFKNGLSKTI---NFHNDLDIDPVVILEICDAQYKEYSLWIKLQN 801 +N+ Y LC + C N+ +I +RK ++ + Y +N ++ S L+I +++IY P +F+ S S + +I ++ KQ ++ FK ++ + L+ E +FF++ F+ ++ +LD+ F + + F C KC+ +F++S G++I IP FPK++ N + Y C KC+ + + + L++ K+G S + + HN ++I+P++ILE + ++ LWIK+ N Sbjct: 143 ENDKEYLLCKSNNCNNLYLITMRKNNIPIFGY-HNPSSFISGHMFLKININLVIYLPNIFQESSNSAIGDIQKSFTNYHKQLHSIQPFKQTVKLYKNHLAPEYKREQFFFKHYFDNMISKLDLSKQFSAVFTNKFSCEKCDINFSRSSVGNSISTSLIPSEFPKYSNNVVSYSEKCSKCSTNAQTSISMKSNSKFLAIFLKHGYSGSKLSDSLHN-IEIEPILILEKSEDSKTKFILWIKIDN 383 The following BLAST results are available for this feature:
BLAST of SMED30014946 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30014946 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30014946 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30014946 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30014946 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014946 ID=SMED30014946|Name=SMED30014946|organism=Schmidtea mediterranea sexual|type=transcript|length=1603bpback to top protein sequence of SMED30014946-orf-1 >SMED30014946-orf-1 ID=SMED30014946-orf-1|Name=SMED30014946-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=265bp MDCCKLCFKQLSYFNQNEVVYSLCSNFLCKNIIINIRKESVAVEEYVYNIback to top Annotated Terms
The following terms have been associated with this transcript:
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