Low density lipoprotein-receptor, class A,domain-containing protein

Overview
NameLow density lipoprotein-receptor, class A,domain-containing protein
Smed IDSMED30014866
Length (bp)714
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014866) t-SNE clustered cells

Violin plots show distribution of expression levels for Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014866) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014866) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014866) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30014866

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30014866SMESG000033681.1 SmedASXL_005282SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30014866SMESG000033681.1 dd_Smed_v4_5576_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014866SMESG000033681.1 dd_Smed_v4_5576_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014866SMESG000033681.1 dd_Smed_v4_5576_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014866SMESG000033681.1 dd_Smed_v4_5576_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014866SMESG000033681.1 Contig41452newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014866SMESG000033681.1 Contig41452uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30014866SMESG000033681.1 dd_Smed_v6_5576_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30014866SMESG000033681.1 dd_Smed_v4_5576_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30014866SMESG000033681.1 dd_Smed_v4_5576_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Celegans
Match: F58H1.7 (NMDA receptor auxiliary protein [Source:UniProtKB/TrEMBL;Acc:G5EEF9])

HSP 1 Score: 77.0258 bits (188), Expect = 2.500e-17
Identity = 49/184 (26.63%), Postives = 88/184 (47.83%), Query Frame = 3
Query:  123 IVFVYVYATEC-FNIFE----KRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPA----IIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGF 620
            IV +    T C F+I +    KR + +   CP   P+ C + G C+   ++CDG PDC ++YDE+ ++C A  RPP       ++  ++   + F+  +F  K          +  +A  L ++PT D FA  + L+  + +++ +VME + +G   +L   E       D      ++ +TGF
Sbjct:    5 IVLMCAIHTACSFSIRDIINGKRGADAATPCPTWHPFACPS-GECVPIKYLCDGSPDCSDEYDENKSMCTAATRPPVEETQAFLKALMSAHGKDFLVKVFGPKAKAELSGMGGVDKVAVALSQTPTADLFASEMKLDDGETQHMLEVMEGILNGSTDELTSNEA-----ADFRFFVQKLQETGF 182          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Celegans
Match: F58H1.7 (NMDA receptor auxiliary protein [Source:UniProtKB/TrEMBL;Acc:G5EEF9])

HSP 1 Score: 73.559 bits (179), Expect = 6.798e-16
Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 3
Query:  198 CPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPA----IIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGF 620
            CP   P+ C + G C+   ++CDG PDC ++YDE+ ++C A  RPP       ++  ++   + F+  +F  K          +  +A  L ++PT D FA  + L+  + +++ +VME + +G   +L   E       D      ++ +TGF
Sbjct:   37 CPTWHPFACPS-GECVPIKYLCDGSPDCSDEYDENKSMCTAATRPPVEETQAFLKALMSAHGKDFLVKVFGPKAKAELSGMGGVDKVAVALSQTPTADLFASEMKLDDGETQHMLEVMEGILNGSTDELTSNEA-----ADFRFFVQKLQETGF 184          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Match: CG31221 (gene:FBgn0051221 transcript:FBtr0083762)

HSP 1 Score: 88.5817 bits (218), Expect = 3.121e-21
Identity = 47/160 (29.38%), Postives = 76/160 (47.50%), Query Frame = 3
Query:  183 RSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPP----AIIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            +    C    P+ C   GNC+S  ++CDG PDC + YDED  LC A  RPP    A  ++  I      ++  +F  K          ++ +A  L ES TI+DF  ++ L +   +++R V  AV +G    L  + + +    D+     ++V TGF+
Sbjct:   59 KRSEACHPYEPFKCPGDGNCISIQYLCDGAPDCSDGYDEDMRLCTAAKRPPVEETASFLQSLIASHGPNYLEKLFGSKARDALSPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSLGIKDSELGDVKFFLEKLVNTGFL 218          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Match: CG31221 (gene:FBgn0051221 transcript:FBtr0083763)

HSP 1 Score: 88.5817 bits (218), Expect = 3.121e-21
Identity = 47/160 (29.38%), Postives = 76/160 (47.50%), Query Frame = 3
Query:  183 RSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPP----AIIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            +    C    P+ C   GNC+S  ++CDG PDC + YDED  LC A  RPP    A  ++  I      ++  +F  K          ++ +A  L ES TI+DF  ++ L +   +++R V  AV +G    L  + + +    D+     ++V TGF+
Sbjct:   59 KRSEACHPYEPFKCPGDGNCISIQYLCDGAPDCSDGYDEDMRLCTAAKRPPVEETASFLQSLIASHGPNYLEKLFGSKARDALSPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSLGIKDSELGDVKFFLEKLVNTGFL 218          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Match: CG31221 (gene:FBgn0051221 transcript:FBtr0330030)

HSP 1 Score: 88.5817 bits (218), Expect = 7.830e-21
Identity = 47/160 (29.38%), Postives = 76/160 (47.50%), Query Frame = 3
Query:  183 RSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPP----AIIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            +    C    P+ C   GNC+S  ++CDG PDC + YDED  LC A  RPP    A  ++  I      ++  +F  K          ++ +A  L ES TI+DF  ++ L +   +++R V  AV +G    L  + + +    D+     ++V TGF+
Sbjct:   59 KRSEACHPYEPFKCPGDGNCISIQYLCDGAPDCSDGYDEDMRLCTAAKRPPVEETASFLQSLIASHGPNYLEKLFGSKARDALSPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSLGIKDSELGDVKFFLEKLVNTGFL 218          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Match: sp|P85831|PROH4_APIME (Prohormone-4 OS=Apis mellifera OX=7460 PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 3.844e-21
Identity = 48/160 (30.00%), Postives = 78/160 (48.75%), Query Frame = 3
Query:  183 RSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPP----AIIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            RSG+ C    P+ C     C+S  ++CDG PDC + YDED+ LC A  RPP    A  ++  +      ++  +F  K          +  +A  L ES TI+DF  ++ L +   +++R V  AV +G    L  + + +    D+     ++VKTGF+
Sbjct:   42 RSGDACRPYEPFKCPGDDTCISIQYLCDGAPDCQDGYDEDSRLCTAAKRPPVEETASFLQSLLASHGPNYLEKLFGTKARDTLKPLGGVNTVAIALSESQTIEDFGAALHLLRTDLEHLRSVFMAVENGDLGMLKSIGIKDSELGDVKFFLEKLVKTGFL 201          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Match: sp|E2AX35|PROH4_CAMFO (IDLSRF-like peptide OS=Camponotus floridanus OX=104421 GN=EAG_01737 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.500e-20
Identity = 47/175 (26.86%), Postives = 84/175 (48.00%), Query Frame = 3
Query:  138 VYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPP----AIIMEQFITKWKEWFIPNIFSDKP---------IKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            + A +    +    ++  + C    P+ C   G C+S  ++CDG PDC + YDED+ LC A  RPP    A  ++  +      ++  +F +K          ++ +A  L ES TI+DF  ++ L +   +++R V  AV +G    L  + + +    D+     ++VKTGF+
Sbjct:   26 IMAIDLSRFYGHINTKRSDACHPYEPFKCPGDGLCISIQYLCDGAPDCQDGYDEDSRLCTAAKRPPVEETATFLQSLLASHGPNYLEKLFGNKARDTLKPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSIGIKDSELGDVKFFLEKLVKTGFL 200          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Match: sp|A0A0F7YYX3|CPROH_CONVC (Neuropeptide prohormone-4 OS=Conus victoriae OX=319920 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.018e-18
Identity = 42/145 (28.97%), Postives = 71/145 (48.97%), Query Frame = 3
Query:  198 CPE----SAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGF 620
            CPE    ++P+ C+    CL    +C+G  DC    DED ALC A  RP   ++ +F+   ++W +PN+F+D    ++A  L E+ ++ D    + L     +N+R        G    L+ + MP+RAW ++  L   +   G 
Sbjct:   47 CPEDCDIASPFKCEESPTCLRLFQVCNGRWDCEHGSDEDNALCAAVLRPLECMIWEFLEGQRDWILPNLFNDANTDLVAHALHEAYSMGDLQSYLNLTDQNIENIRNSTRGAIVGDPRPLMALGMPDRAWPEVMYLLKELYNLGL 191          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A158QJB1 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS11424 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 5.121e-54
Identity = 79/154 (51.30%), Postives = 108/154 (70.13%), Query Frame = 3
Query:  168 EKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKN 629
            E R     N CPE+ PWPC+T   CL+FDFICDGE DC + YDED  LCIAK RPP   M QFITK+ +W IP+I  +    I+A +L+ESPTI+D+A +  LNK+Q+ N+  V+E V   +++ L+L+ MP  AW++LY LF+RI+ +GF+KN
Sbjct:    1 EHRNVTLANTCPETRPWPCRTGHRCLAFDFICDGEKDCADGYDEDEELCIAKQRPPVEYMVQFITKYHDWLIPDILGEGSPFILAKMLVESPTIEDYASAAHLNKEQFSNLYSVLEGVYLRKEMQLVLLGMPTGAWSELYYLFNRIITSGFVKN 154          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A158QEM3 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS7631 PE=4 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 3.538e-52
Identity = 75/154 (48.70%), Postives = 108/154 (70.13%), Query Frame = 3
Query:  168 EKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKN 629
            E R     N CPE+ PWPC+T   CL+FDFICDGE DC + YDE+  LCIAK RPP   + QFITK+ +W IP+I  +    ++A +L++SPTI+D+A +V LNK+Q+ N+   +E V   +++ L+L+ MP  AW++LY LF+RI+ +GF+KN
Sbjct:    1 EHRNITLANTCPETRPWPCRTGNRCLAFDFICDGEKDCADGYDENEELCIAKQRPPVEYLVQFITKYHDWLIPDILGEGSPLVLAKMLVQSPTIEDYASAVHLNKEQFSNLYSALEGVYLRKEMQLVLLGMPLGAWSELYYLFNRIITSGFVKN 154          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A564Z7Z7 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS12934 PE=4 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 4.203e-52
Identity = 75/154 (48.70%), Postives = 108/154 (70.13%), Query Frame = 3
Query:  168 EKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKN 629
            E R     N CPE+ PWPC+T   CL+FDFICDGE DC + YDE+  LCIAK RPP   + QFITK+ +W IP+I  +    ++A +L++SPTI+D+A +V LNK+Q+ N+   +E V   +++ L+L+ MP  AW++LY LF+RI+ +GF+KN
Sbjct:   37 EHRNITLANTCPETRPWPCRTGNRCLAFDFICDGEKDCADGYDENEELCIAKQRPPVEYLVQFITKYHDWLIPDILGEGSPLVLAKMLVQSPTIEDYASAVHLNKEQFSNLYSALEGVYLRKEMQLVLLGMPLGAWSELYYLFNRIITSGFVKN 190          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A094ZWF8 (Uncharacterized protein OS=Schistosoma haematobium OX=6185 GN=MS3_05853 PE=4 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 3.011e-50
Identity = 76/167 (45.51%), Postives = 112/167 (67.07%), Query Frame = 3
Query:  129 FVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKN 629
            F+ ++AT  ++I+  +    GN CPES PWPC+TP  CLSFDFICDGEPDCP++YDED  +C AK RP    +  FI +++ W +P        + +A  L+ES T+ D+A++  LN+ Q KN+ K+ME V+ G++++L L+ MP  AW++LY LF RI  +GF+ N
Sbjct:    8 FMKLFATVQYSIYFIQAVERGNDCPESQPWPCRTPRICLSFDFICDGEPDCPDEYDEDPEMCTAKLRPAQEHLAGFIHRYQNWLVPKFLGYGTPEELAAKLVESKTVSDYARAAKLNETQLKNLIKLMEGVKSGKEMELFLLGMPLGAWSELYTLFHRIYISGFLDN 174          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A267EU33 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004733g1 PE=4 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 4.840e-50
Identity = 78/157 (49.68%), Postives = 105/157 (66.88%), Query Frame = 3
Query:  153 CFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            C+    ++ S  GN CP++APWPCK+ G CLSF FICDG  DC + YDED ALC AKDRP +II++ FI ++  W IPNI  +   + +A  L E   + D+A +VGLNK Q + +  VME  RDGR +DL+L  MPE A+ + Y LF RIV++GF+
Sbjct:   23 CYRQLVRKGSALGNDCPKTAPWPCKS-GQCLSFSFICDGRQDCEDGYDEDTALCTAKDRPASIILKSFIARFHNWLIPNILGEGTPEELAKQLTEQLNVRDYASAVGLNKQQLRKLVLVMEYARDGRILDLILDGMPEEAYREAYALFGRIVESGFL 178          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000033681.1 (SMESG000033681.1)

HSP 1 Score: 373.244 bits (957), Expect = 2.185e-133
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 3
Query:   96 MSGYSYFILIVFVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKNNA 635
            MSGYSYFILIVFVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKNNA
Sbjct:    1 MSGYSYFILIVFVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIKNNA 180          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000021406.1 (SMESG000021406.1)

HSP 1 Score: 188.734 bits (478), Expect = 1.335e-60
Identity = 81/152 (53.29%), Postives = 109/152 (71.71%), Query Frame = 3
Query:  171 KRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIK 626
            K    SGNGCPES PWPCKTPG CLSFDFICDGEPDCP+KYDED +LC AK+RPP  ++++F+ K++ W IP          IAT LIES   ++++  + L + Q K++  ++ AV++GRQI+L++  MP RAW +LYLLF RI  +GF+K
Sbjct:   26 KTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 177          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000076971.1 (SMESG000076971.1)

HSP 1 Score: 189.119 bits (479), Expect = 1.447e-60
Identity = 89/168 (52.98%), Postives = 119/168 (70.83%), Query Frame = 3
Query:  120 LIVFVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            +I+ V++     + I +  +   GN CPES PWPCK+ G CLSFDFICD E DCP+KYDED ALCIAK+RPP +++EQF+ KWK+  IP +F +   K +A  L+ES TI D+ K+  L + Q+KN  KVMEAVR  RQID L+M +P+RAW D+Y LF R +K+GF+
Sbjct:   13 MILNVHIIILRAYEIEKHMKRNPGNVCPESKPWPCKS-GLCLSFDFICDEEFDCPDKYDEDPALCIAKNRPPVVLLEQFLMKWKDSLIPKVFGNGLPKDLADKLVESLTILDYKKAAHLTESQFKNFYKVMEAVRKNRQIDWLMMGLPDRAWNDMYNLFRRFLKSGFL 179          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000076971.1 (SMESG000076971.1)

HSP 1 Score: 188.348 bits (477), Expect = 1.542e-60
Identity = 89/168 (52.98%), Postives = 119/168 (70.83%), Query Frame = 3
Query:  120 LIVFVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFI 623
            +I+ V++     + I +  +   GN CPES PWPCK+ G CLSFDFICD E DCP+KYDED ALCIAK+RPP +++EQF+ KWK+  IP +F +   K +A  L+ES TI D+ K+  L + Q+KN  KVMEAVR  RQID L+M +P+RAW D+Y LF R +K+GF+
Sbjct:    1 MILNVHIIILRAYEIEKHMKRNPGNVCPESKPWPCKS-GLCLSFDFICDEEFDCPDKYDEDPALCIAKNRPPVVLLEQFLMKWKDSLIPKVFGNGLPKDLADKLVESLTILDYKKAAHLTESQFKNFYKVMEAVRKNRQIDWLMMGLPDRAWNDMYNLFRRFLKSGFL 167          
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000021406.1 (SMESG000021406.1)

HSP 1 Score: 188.348 bits (477), Expect = 1.834e-60
Identity = 81/152 (53.29%), Postives = 109/152 (71.71%), Query Frame = 3
Query:  171 KRESRSGNGCPESAPWPCKTPGNCLSFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIFSDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDLLLMEMPERAWTDLYLLFSRIVKTGFIK 626
            K    SGNGCPES PWPCKTPG CLSFDFICDGEPDCP+KYDED +LC AK+RPP  ++++F+ K++ W IP          IAT LIES   ++++  + L + Q K++  ++ AV++GRQI+L++  MP RAW +LYLLF RI  +GF+K
Sbjct:   23 KTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 174          
The following BLAST results are available for this feature:
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
F58H1.72.500e-1726.63NMDA receptor auxiliary protein [Source:UniProtKB... [more]
F58H1.76.798e-1626.62NMDA receptor auxiliary protein [Source:UniProtKB... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CG312213.121e-2129.38gene:FBgn0051221 transcript:FBtr0083762[more]
CG312213.121e-2129.38gene:FBgn0051221 transcript:FBtr0083763[more]
CG312217.830e-2129.38gene:FBgn0051221 transcript:FBtr0330030[more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P85831|PROH4_APIME3.844e-2130.00Prohormone-4 OS=Apis mellifera OX=7460 PE=1 SV=1[more]
sp|E2AX35|PROH4_CAMFO1.500e-2026.86IDLSRF-like peptide OS=Camponotus floridanus OX=10... [more]
sp|A0A0F7YYX3|CPROH_CONVC1.018e-1828.97Neuropeptide prohormone-4 OS=Conus victoriae OX=31... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A158QJB15.121e-5451.30Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
A0A158QEM33.538e-5248.70Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A564Z7Z74.203e-5248.70Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A094ZWF83.011e-5045.51Uncharacterized protein OS=Schistosoma haematobium... [more]
A0A267EU334.840e-5049.68Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000033681.12.185e-133100.00SMESG000033681.1[more]
SMESG000021406.11.335e-6053.29SMESG000021406.1[more]
SMESG000076971.11.447e-6052.98SMESG000076971.1[more]
SMESG000076971.11.542e-6052.98SMESG000076971.1[more]
SMESG000021406.11.834e-6053.29SMESG000021406.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014866 ID=SMED30014866|Name=Low density lipoprotein-receptor, class A,domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=714bp
CGCGATTATCATAGAGTTTCCTCAACAGTCAAATTCTTTCACATTCATTT
TTTTTCAAATCGAGACAATTTCGTTCAATTAGCTCCTCGCAAAAAATGAG
TGGATACTCTTATTTTATTCTGATTGTATTCGTCTACGTATACGCAACAG
AATGTTTCAATATTTTTGAAAAAAGAGAAAGCCGATCGGGAAATGGATGT
CCAGAAAGTGCGCCATGGCCTTGCAAAACTCCTGGAAATTGTTTATCATT
TGACTTTATATGTGACGGTGAACCGGACTGTCCAGAAAAATATGATGAAG
ATGCAGCTTTATGCATTGCAAAAGATCGCCCGCCTGCAATCATTATGGAA
CAGTTTATTACAAAATGGAAAGAGTGGTTTATTCCGAACATCTTTTCCGA
CAAACCCATTAAAATAATTGCAACGCTTTTGATTGAGAGTCCAACCATTG
ATGACTTTGCTAAAAGTGTTGGCTTGAATAAAGATCAATATAAAAATGTA
AGAAAAGTCATGGAGGCCGTTCGTGACGGAAGACAAATTGATCTACTGTT
GATGGAAATGCCAGAGAGAGCTTGGACCGACTTATATCTGCTATTTTCAA
GAATCGTTAAAACCGGATTCATTAAAAATAATGCCTAACACTGCAAAAGT
GTAAAACATAAATTTGATGATGTAACTTGAATAAAATATTAAACATATTA
TGGCATAGTTTCCA
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protein sequence of SMED30014866-orf-1

>SMED30014866-orf-1 ID=SMED30014866-orf-1|Name=SMED30014866-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=181bp
MSGYSYFILIVFVYVYATECFNIFEKRESRSGNGCPESAPWPCKTPGNCL
SFDFICDGEPDCPEKYDEDAALCIAKDRPPAIIMEQFITKWKEWFIPNIF
SDKPIKIIATLLIESPTIDDFAKSVGLNKDQYKNVRKVMEAVRDGRQIDL
LLMEMPERAWTDLYLLFSRIVKTGFIKNNA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007218neuropeptide signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000028Category 2 cell
PLANA:0000099neuron
PLANA:0000240body wall musculature
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036055LDL_receptor-like_sf
IPR002172LDrepeatLR_classA_rpt
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.40.128.620coord: 34..118
e-value: 5.6E-7
score: 32.0
NoneNo IPR availablePANTHERPTHR20967UNCHARACTERIZEDcoord: 24..93
NoneNo IPR availableTMHMMTMhelixcoord: 5..24
IPR002172Low-density lipoprotein (LDL) receptor class A repeatPROSITEPS50068LDLRA_2coord: 34..74
score: 9.75
IPR002172Low-density lipoprotein (LDL) receptor class A repeatCDDcd00112LDLacoord: 35..69
e-value: 0.0013667
score: 32.949
IPR036055LDL receptor-like superfamilySUPERFAMILYSSF57424LDL receptor-like modulecoord: 40..73