DNA replication ATP-dependent helicase/nuclease DNA2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014833 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Human
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939]) HSP 1 Score: 403.675 bits (1036), Expect = 1.484e-121 Identity = 318/977 (32.55%), Postives = 492/977 (50.36%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQA---MKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIV------QNSKKSIRN-VTFESSSL-SGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS------ELDRSVIFLNY----QPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM---STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQII 3381
W V GDI + S+ W I+ G L++ P L+S TS+ S+ C R+ VL F S + + M+IG+ +H +FQ+ +++E+A + ++ L Y + +EI +E+E Y P KW + K + +MQ+ +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH QV+LY L+ S + + + GLLLY+K G + + N +K+RN++ + I K + Q T LP +I + C+ C + C ++SR + DCS M I++ T HL + E+F W ++ LE S+ + +N +NI + + S I N + E + +L F P + GD +IVS ++ A+ GYV E T + + + + T R+D N + P+ NLS LM+ + + +KLRD I+ + P F +++ L K I+K N QR+ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V ++ T I K ++L L + +V TC+ NH SR FD I+DEA QI+ P+ + PL +F+L GD QLPPLV N AR G+ +SLF +L + + ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A I + H + LEF+ + E + V FLN P VE + N EAK+ + L F+K G SDIG+I PY QQ ++I ++ S VE+NT+D+YQGRDKSIV++SFV + + +L D RRLNVAI+RAKHKLI++G + L+ Y P++ + + L + ++I
Sbjct: 86 WCSVPVEPGDIIHLEGDCTSDT-WIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRS-SDPATRQMLIGTVLHEVFQKAIN-NSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLL-SQERRADPEAGLLLYLKTG-QMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNH-QNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLS---KLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLF----FSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQ--NKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVS-NVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNK------DGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLN-CYPPLEKLLNHLNSEKLI 1039
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Human
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939]) HSP 1 Score: 214.157 bits (544), Expect = 1.111e-57 Identity = 170/587 (28.96%), Postives = 277/587 (47.19%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQA---MKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNII------VQNSKKSIRN-VTFESSSL-SGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIR 2259
W V GDI + S+ W I+ G L++ P L+S TS+ S+ C R+ VL F S + + M+IG+ +H +FQ+ +++E+A + ++ L Y + +EI +E+E Y P KW + K + +MQ+ +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH QV+LY L+ S + + + GLLLY+K G + + N +K+RN++ + I K + Q T LP +I + C+ C + C ++SR + DCS M I++ T HL + E+F W ++ LE S+ + +N +NI ++ S I N + E + +L F P + GD +IVS ++ A+ GYV E T + + + + T R+D N + P+ NLS LM+ + + +KLRD I+ + P F +++ L K I+K N QR+ + + SKDY LI G PG+GKT+ I ++R
Sbjct: 86 WCSVPVEPGDIIHLEGDCTSDT-WIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRS-SDPATRQMLIGTVLHEVFQKAIN-NSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLL-SQERRADPEAGLLLYLKTG-QMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNH-QNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLS---KLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVR 663
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Human
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939]) HSP 1 Score: 214.157 bits (544), Expect = 1.111e-57 Identity = 170/587 (28.96%), Postives = 277/587 (47.19%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQA---MKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNII------VQNSKKSIRN-VTFESSSL-SGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIR 2259
W V GDI + S+ W I+ G L++ P L+S TS+ S+ C R+ VL F S + + M+IG+ +H +FQ+ +++E+A + ++ L Y + +EI +E+E Y P KW + K + +MQ+ +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH QV+LY L+ S + + + GLLLY+K G + + N +K+RN++ + I K + Q T LP +I + C+ C + C ++SR + DCS M I++ T HL + E+F W ++ LE S+ + +N +NI ++ S I N + E + +L F P + GD +IVS ++ A+ GYV E T + + + + T R+D N + P+ NLS LM+ + + +KLRD I+ + P F +++ L K I+K N QR+ + + SKDY LI G PG+GKT+ I ++R
Sbjct: 86 WCSVPVEPGDIIHLEGDCTSDT-WIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRS-SDPATRQMLIGTVLHEVFQKAIN-NSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLL-SQERRADPEAGLLLYLKTG-QMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNH-QNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLS---KLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVR 663
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Human
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939]) HSP 1 Score: 211.075 bits (536), Expect = 1.320e-55 Identity = 169/586 (28.84%), Postives = 276/586 (47.10%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQA---MKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIV------QNSKKSIRN-VTFESSSL-SGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMI 2256
W V GDI + S+ W I+ G L++ P L+S TS+ S+ C R+ VL F S + + M+IG+ +H +FQ+ +++E+A + ++ L Y + +EI +E+E Y P KW + K + +MQ+ +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH QV+LY L+ S + + + GLLLY+K G + + N +K+RN++ + I K + Q T LP +I + C+ C + C ++SR + DCS M I++ T HL + E+F W ++ LE S+ + +N +NI + + S I N + E + +L F P + GD +IVS ++ A+ GYV E T + + + + T R+D N + P+ NLS LM+ + + +KLRD I+ + P F +++ L K I+K N QR+ + + SKDY LI G PG+GKT+ I ++
Sbjct: 86 WCSVPVEPGDIIHLEGDCTSDT-WIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRS-SDPATRQMLIGTVLHEVFQKAIN-NSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLL-SQERRADPEAGLLLYLKTG-QMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNH-QNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLS---KLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLV 662 HSP 2 Score: 80.1073 bits (196), Expect = 1.995e-14 Identity = 59/136 (43.38%), Postives = 88/136 (64.71%), Query Frame = 1
Query: 2989 NKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM---STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 3387
N EAK+ + L F+K G SDIG+I PY QQ ++I ++ S VE+NT+D+YQGRDKSIV++SFV + + +L D RRLNVAI+RAKHKLI++G + L+ Y P++ + + L + ++IS+
Sbjct: 675 NVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNK------DGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLN-CYPPLEKLLNHLNSEKLISL 803
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Human
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939]) HSP 1 Score: 179.104 bits (453), Expect = 1.523e-48 Identity = 143/375 (38.13%), Postives = 207/375 (55.20%), Query Frame = 1
Query: 2308 SAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSSEL------DRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM---STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
SAV NIL KL +I FL L V ++ T I K ++L L + +V TC+ NH SR FD I+DEA QI+ P+ + PL +F+L GD QLPPLV N AR G+ +SLF +L + + ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A I + H + LEF+ + L + V FLN P VE + N EAK+ + L F+K G SDIG+I PY QQ ++I ++ S VE+NT+D+YQGRDKSIV++SFV + G E +L D RRLNVAI+RAKHKLI++G + L+ Y P++ + + L + ++IS
Sbjct: 2 SAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLF----FSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQ--NKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVS-NVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNKD-GTVGE-----LLKDWRRLNVAITRAKHKLILLGCVPSLN-CYPPLEKLLNHLNSEKLIS 362
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Celegans
Match: dna-2 (Yeast DNA helicase/endonuclease family [Source:UniProtKB/TrEMBL;Acc:Q9U3F2]) HSP 1 Score: 342.813 bits (878), Expect = 5.543e-100 Identity = 282/945 (29.84%), Postives = 472/945 (49.95%), Query Frame = 1
Query: 643 LHLDGMWEDTVVHVGDICSIVDPIK-SNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQ-PSQLITFYESGWTDEEILREIEVYFPRILKWINRNL--------SKKPEISK---MQVIDVEENIWSTKYGIKGKIDVTLQTNE--STIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSK---KSIRNVTFESSSLSGIN-------------------FLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVE-SPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLF--DCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREI---NFLYLNGNHSVCEKLKNRTLSYILPEKKFENLF-NFHNLIENIDVVGVTCLSANHAAL-SRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEHESINSLEFWK---CQCCSSELDRSVIFLNYQPSF-----VEADE--IYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN-MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIG 3306
++L W + + G +++ + + +N+ GI+++NP LV T+V + FC RK VL F SNKAM++G+ +H IFQ + + E ++ K +KQ P +T + E++ YF I WIN++ ++P SK ++V D+EENIW +K G+KGKIDVT++T LELKTG+++ S EHT QVLLY +M S + E I G +LY+KDGA + + ++ ++ RN+L+ + F +N LP + R C C++ ++C+ + + +N S + MK+ ++ HL + E+ +WI I E+ E + + QN KS++ E + LS ++ FL N P F GD ++S + K +AI + E S I+ IS++K + +D +S+ + L L L+ EIG++LR+ +V PP+ + A+K I N +QR+ ++ A+ ++D+++++G PGSGKT+ I +I+ V KVL++++THSAV NIL KL +E+ L L + S+ + +K TL L + E + +++ +V TC L S FDVVI+DEA + P+ + + KF+L GD QL PLV + A+ G S KL + +HP + L QYRMN EI L + + Y N++ C + V+ +++ + I +++ + S ++ S +FL+ Q + E E N EAK+ EL +QF+ G +IGV+ Y +Q + IR +++ E+E+NTID YQGR+K ++I S ++ K+ +L D+RR+NVA++RA+ KL+++G
Sbjct: 131 VYLQDHWSEMSIEAGSTINLIGAQAWGDRDFLVNDDVGIVILNPDALVPCTAVASATFCARKTVLNDKFK-FGNASNKAMLLGTIMHEIFQTAITSKKRPVME-NDLMKIWNKQAPKYAEELVALSFTPSCLDAELQPYFSIICGWINKHYPLSNSFFSKREPLPSKSELLEVYDIEENIWDSKLGLKGKIDVTMRTKSRGGMESLELKTGKSSCSSEHTGQVLLYCMMQSSRYEQPIGPGNILYLKDGASHCV-TPRAADLLGVLQQRNKLS---VHFEDPTTSN---LPDPRQDSRFCDKCDHKTMCSFYQKTEENFSKSNDKMKNFAENEMAHLEQNHIEYAANWIRWISAEWKCER------ERMTSQNKDLWLKSVQERVEEGTCLSDLHPVSEEMSNSQKIIISFSRKFLKSSNTPT-----FRPGDVCLLS--NKKHIAIGFALIDEVSDDQQIVKISTDKTIKSRYEAPFHLDKYTSMSTHSTTLGNLVYLLQNDEIGKRLRNILVDLLPPIIMDATGFNLTPAIKKIIIKAKLNNEQRKAVVHALATEDFMMVEGLPGSGKTTLISVLIQCLVATNKKVLLAAFTHSAVDNILTKL-TKEVAAEKILRLGSSSSIKDDIKKMTLKAKLENETSEEYYAAVRKVMKTTPIVACTCHHVPRELLFSYRHFDVVIVDEASMVLEPLLLP----VLATSNKFVLVGDCKQLTPLVVSRKAKQEGAGISTMEKL-QQSHPGVVVSLTSQYRMNREISVLSSKLFYENRLICGNESVSRSSLDRTGDYIVAMDDGSDHIRKALSGDIKDSCVFLDTQSTINSKMQCEDGEGGGMCNDGEAKLISELCQQFVMSGVKPHEIGVMSAYRRQVDHIRGILNSDELEVNTIDSYQGREKRVIIWSLTWTNNSTKKS------ELLKDERRVNVALTRARQKLVVVG 1041
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Celegans
Match: smg-2 (Regulator of nonsense transcripts 1 [Source:UniProtKB/Swiss-Prot;Acc:O76512]) HSP 1 Score: 117.087 bits (292), Expect = 3.491e-26 Identity = 127/432 (29.40%), Postives = 196/432 (45.37%), Query Frame = 1
Query: 2182 IFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGM---KVLVSSYTHSAVSNILQKL---------------DAREINFLYLNGNHSV-----CEKLKNRTLSYILPEKKFENLFNF--------HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLP---VSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYN----NQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN-MSTM---------EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQN 3330
+ ++ LI+G PG+GKT +V+ ++ L+ VLV S ++ AV ++ +K+ + E YL H + E K L E +F++ + H L+ DV+ TC SA A LS+++ V+IDE+ Q T P VSIM ++ + +L GD QL P+V A I GL QSLF +L P L++QYRM+ + + + + Y+ N +T + + FW C S EL S + N+ EA +LV + +K G IGVI PY Q+ I N M T VEI ++D +QGR+K +I++ V +D LG L+D RRLNVAI+RAK+ L+++GN L+++
Sbjct: 457 VLTRPLSLIQGPPGTGKT--VVSATIVYHLVQKTEGNVLVCSPSNIAVDHLAEKIHKTGLKVVRLCARSREHSETTVPYLTLQHQLKVMGGAELQKLIQLKDEAGELEFKDDLRYMQLKRVKEHELLAAADVICCTCSSAADARLSKIRTRTVLIDESTQATEPEILVSIMRGVR------QLVLVGDHCQLGPVVICKKAAIAGLSQSLFERLVLLGIRP---FRLQVQYRMHPVLSEFPSNVFYDGSLQNGVTENDRHMTGVDWHWPKPNKPAFFWHC-SGSEELSASGTS-------------FLNRTEAANVEKLVSKLIKAGVQPHQIGVITPYEGQRSFIVNYMHTQGTLNSKLYENVEIASVDAFQGREKDYIIVTCVRSNDILG-------IGFLSDPRRLNVAITRAKYGLVLVGNAKVLARH 856
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Celegans
Match: smg-2 (Regulator of nonsense transcripts 1 [Source:UniProtKB/Swiss-Prot;Acc:O76512]) HSP 1 Score: 117.087 bits (292), Expect = 3.491e-26 Identity = 127/432 (29.40%), Postives = 196/432 (45.37%), Query Frame = 1
Query: 2182 IFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGM---KVLVSSYTHSAVSNILQKL---------------DAREINFLYLNGNHSV-----CEKLKNRTLSYILPEKKFENLFNF--------HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLP---VSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYN----NQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN-MSTM---------EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQN 3330
+ ++ LI+G PG+GKT +V+ ++ L+ VLV S ++ AV ++ +K+ + E YL H + E K L E +F++ + H L+ DV+ TC SA A LS+++ V+IDE+ Q T P VSIM ++ + +L GD QL P+V A I GL QSLF +L P L++QYRM+ + + + + Y+ N +T + + FW C S EL S + N+ EA +LV + +K G IGVI PY Q+ I N M T VEI ++D +QGR+K +I++ V +D LG L+D RRLNVAI+RAK+ L+++GN L+++
Sbjct: 457 VLTRPLSLIQGPPGTGKT--VVSATIVYHLVQKTEGNVLVCSPSNIAVDHLAEKIHKTGLKVVRLCARSREHSETTVPYLTLQHQLKVMGGAELQKLIQLKDEAGELEFKDDLRYMQLKRVKEHELLAAADVICCTCSSAADARLSKIRTRTVLIDESTQATEPEILVSIMRGVR------QLVLVGDHCQLGPVVICKKAAIAGLSQSLFERLVLLGIRP---FRLQVQYRMHPVLSEFPSNVFYDGSLQNGVTENDRHMTGVDWHWPKPNKPAFFWHC-SGSEELSASGTS-------------FLNRTEAANVEKLVSKLIKAGVQPHQIGVITPYEGQRSFIVNYMHTQGTLNSKLYENVEIASVDAFQGREKDYIIVTCVRSNDILG-------IGFLSDPRRLNVAITRAKYGLVLVGNAKVLARH 856
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Celegans
Match: smg-2 (Regulator of nonsense transcripts 1 [Source:UniProtKB/Swiss-Prot;Acc:O76512]) HSP 1 Score: 117.087 bits (292), Expect = 3.491e-26 Identity = 127/432 (29.40%), Postives = 196/432 (45.37%), Query Frame = 1
Query: 2182 IFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGM---KVLVSSYTHSAVSNILQKL---------------DAREINFLYLNGNHSV-----CEKLKNRTLSYILPEKKFENLFNF--------HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLP---VSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYN----NQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN-MSTM---------EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQN 3330
+ ++ LI+G PG+GKT +V+ ++ L+ VLV S ++ AV ++ +K+ + E YL H + E K L E +F++ + H L+ DV+ TC SA A LS+++ V+IDE+ Q T P VSIM ++ + +L GD QL P+V A I GL QSLF +L P L++QYRM+ + + + + Y+ N +T + + FW C S EL S + N+ EA +LV + +K G IGVI PY Q+ I N M T VEI ++D +QGR+K +I++ V +D LG L+D RRLNVAI+RAK+ L+++GN L+++
Sbjct: 457 VLTRPLSLIQGPPGTGKT--VVSATIVYHLVQKTEGNVLVCSPSNIAVDHLAEKIHKTGLKVVRLCARSREHSETTVPYLTLQHQLKVMGGAELQKLIQLKDEAGELEFKDDLRYMQLKRVKEHELLAAADVICCTCSSAADARLSKIRTRTVLIDESTQATEPEILVSIMRGVR------QLVLVGDHCQLGPVVICKKAAIAGLSQSLFERLVLLGIRP---FRLQVQYRMHPVLSEFPSNVFYDGSLQNGVTENDRHMTGVDWHWPKPNKPAFFWHC-SGSEELSASGTS-------------FLNRTEAANVEKLVSKLIKAGVQPHQIGVITPYEGQRSFIVNYMHTQGTLNSKLYENVEIASVDAFQGREKDYIIVTCVRSNDILG-------IGFLSDPRRLNVAITRAKYGLVLVGNAKVLARH 856
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Celegans
Match: Y106G6D.5 (pep chromosome:WBcel235:I:10119209:10121815:-1 gene:WBGene00014965.1 transcript:Y106G6D.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y106G6D.5) HSP 1 Score: 112.849 bits (281), Expect = 2.338e-25 Identity = 129/476 (27.10%), Postives = 211/476 (44.33%), Query Frame = 1
Query: 2122 KIKHIMKICNPDQRRVIMSAIFSKDYLL-IKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNG----------------NHSVCEKLKN-----RTLSYILPEKK---FENLFNFHNLIENIDVVGVTCLSA-NHAALSRLK-----FDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCE-FNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWK-CQCCSSE--LDRSVIFLNYQ----PSFVEADEIYF-NKFEAKITMELVEQFL-KIGALESDIGVIIPYNQQKEMIRNMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI*CNFGNICLSSLN 3426
KI ++ + NP Q + +A+ ++ LL I+G PG+GKT I ++ + KVLV + TH AV N + R I + NH KL+ T+S P K +E +++ +I + S +++ +L DV+I+DEA Q T P + +P+L K K IL GD QLP +V + A SL KL E F+ + L QYRMN +I+ N + Y NQ+T H +++ + C + L+ ++ +N + S E + F N E + E V + + +G I VI PY Q E +R V++NT+D +QG ++ +VI L + + LN+ RRLNVA++RA+ + ++IGN + N +K ++ L++ ++I FG L + N
Sbjct: 123 KIGNLPETLNPSQVAAVSAAMNTQRNLLCIQGPPGTGKTRVIAEIVHQLMKKKKKVLVCAPTHVAVRNAMDATTGRMIEEMPEEKAEQQLCLLANTKDEFQNHKSASKLEEIKKQLSTMSTSDPLYKKMSYEYYLIRNSIFRSIFYPKLAVFSTLGTSSIQKLPEYHWNADVMIVDEAAQCTEPATWVPVLTTPSCK-KLILVGDQKQLPAVVLSDKAMKGNFKLSLMEKLAEEFSSNNINILLNEQYRMNKKIMNWSNEVFYENQLTA------------HSTVSDITLRDICPNLPEDHVLNNPIMMINMENVKDRSHEEFESHSFTNTDELNLVTEYVNRLVVDLGINPKAIAVISPYYAQIEKLRRSIPFRVDVNTVDAFQGHERQVVIFC-------LVRDNDEGQIGFLNETRRLNVAVTRARRQFVLIGNGRMMKGNKHLLK-LYKHLQSEKVI-----FGPSILETFN 572
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Fly
Match: CG2990 (gene:FBgn0030170 transcript:FBtr0071415) HSP 1 Score: 380.563 bits (976), Expect = 3.684e-113 Identity = 301/986 (30.53%), Postives = 478/986 (48.48%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIV---DPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKP------EISKMQV---IDVEENIWSTKYGIKGKIDVTLQT-----NESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCL-----PSVITNIRLCQSCEYLSLCTMFSRLSQNDSDC----SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLE------------FLSESSNENPIKNIIVQNSK-KSIRNVTFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVV--ESPGTDILIIS------SEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGN---HSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNY------------------------QPSFVE-ADEI-----------YFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMST---MEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIK 3345
W + VGD S++ DP S G + ++ +G V +P +L+S T+VTGSLFC+RK VLQ F G+ N MVIG+ +H + Q+ R + D ++ + + S Y S E+ + + I+ ++ + + +P E+ + Q+ D+EEN+W + G+KGK+DV+++ E IPLELKTGR +FS EH Q+LLY LM S + + GLLLY+K+G R + + + + +RN+L ++ + + + D L P + + C +C Y ++C+ F+ Q DS S +K L+ HL E D+ + HW L+ LE F ++ E + I +++ K + V E L + P + F G+ +++SS ++A+ G++V E+ D+ + SE+ I ID + S N + LG L+ + E Q+LRD IV KPP + K + K I+ N Q+ + A+ + +LLIKG PG+GKT +VA++R+ L+G VL+++ THSAV N++ +L + + L H E++ L+ K + + + +VGVTCL H R +FD I+DEA Q+ P + PL+ KF+L GD QLPP+VR+ AR G D++LF +L + + L LQYRMN I +L N ++Y + CA+D+V+ A E E +N W + ++ L+++V +N + SF E DEI Y N EA I M L+ LK G S IGVI PY Q E+ + +++ ++E NT+DQ+QGRDK+++I S + IL DQRRL VAI+RAK+KLI++G++ L Q Y P +
Sbjct: 111 WNCMPMQVGDTVSLLGKWDP--SAGCYVVDKEQGYCVSHPDFLISGTTVTGSLFCKRKSVLQERFRGLDS-GNSVMVIGTLVHELLQKVLRQKLFDKKHIQSALQEMLASSSLAHLLYASNLIQVEMEDHLMKFIDPIVSFVAQYVKGEPPSVLLPEVYRGQIHEICDIEENLWVPQLGLKGKVDVSVKVKNQRQREEIIPLELKTGRASFSMEHKGQLLLYQLMHSAQGR-DTQSGLLLYLKEGLLREVASGRNEQR-DLLMLRNDLAYWLTREVAIPASKEDPLEQLPLPEPVYHHSACGNCAYNTICSSFA---QKDSSLQLSDSHPLKKLMPQLLEHLSEADHAYVQHWCGLLALEEQHNRQSSHVRSFWTKDPAEREKEGIAIRHLKLVKGQEVILEEGRYRQTLELGEEADPSRDLSLSGFDLGEYVVISSTS--RLAVAAGFIVSIEARRLDLRLERDLSQRYSEETFI--------IDKHDSQSFATFNFTNLGMLLSEGERFQELRDIIVAKKPPEQHKVLPKIILTKGAPILLNLNKVQQNAALRALTTSSHLLIKGLPGTGKTQTLVALVRLLHLLGKSVLITAQTHSAVDNLIMRLLPFGLPMMRLGSGSRIHKQLEEISEERLT-----KDCKTVEELEKALGQPSIVGVTCLGCGHPLFQRRQFDYCIVDEATQVLQPTVLRPLIHC----SKFVLVGDPEQLPPIVRSKEARQRGADETLFQRL---DSEKATAVLSLQYRMNKTITRLANELTYGGDLKCASDEVSGARF-EVELLNEAPRWVQRALTTHLEQAVTLINTGDCLERCQEFVYASQRLVDTCSSIEQSFSEDKDEIRKLTSKKRVSKYTNYCEAGIVMHLLRYLLKSGYEASRIGVIAPYRAQVELFKKLASKLDTDLECNTVDQFQGRDKNLIIYSCSKTGGDFSDMERSREAEILEDQRRLTVAITRAKNKLILLGDIKCLEQ-YGPFR 1064
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Fly
Match: CG2990 (gene:FBgn0030170 transcript:FBtr0112971) HSP 1 Score: 380.563 bits (976), Expect = 3.804e-113 Identity = 301/986 (30.53%), Postives = 479/986 (48.58%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIV---DPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKP------EISKMQV---IDVEENIWSTKYGIKGKIDVTLQT-----NESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCL-----PSVITNIRLCQSCEYLSLCTMFSRLSQNDSDC----SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLE------------FLSESSNENPIKNIIVQNSK-KSIRNVTFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVV--ESPGTDILIIS------SEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGN---HSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNY------------------------QPSFVE-ADEI-----------YFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMST---MEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIK 3345
W + VGD S++ DP S G + ++ +G V +P +L+S T+VTGSLFC+RK VLQ F G+ S +MVIG+ +H + Q+ R + D ++ + + S Y S E+ + + I+ ++ + + +P E+ + Q+ D+EEN+W + G+KGK+DV+++ E IPLELKTGR +FS EH Q+LLY LM S + + GLLLY+K+G R + + + + +RN+L ++ + + + D L P + + C +C Y ++C+ F+ Q DS S +K L+ HL E D+ + HW L+ LE F ++ E + I +++ K + V E L + P + F G+ +++SS ++A+ G++V E+ D+ + SE+ I ID + S N + LG L+ + E Q+LRD IV KPP + K + K I+ N Q+ + A+ + +LLIKG PG+GKT +VA++R+ L+G VL+++ THSAV N++ +L + + L H E++ L+ K + + + +VGVTCL H R +FD I+DEA Q+ P + PL+ KF+L GD QLPP+VR+ AR G D++LF +L + + L LQYRMN I +L N ++Y + CA+D+V+ A E E +N W + ++ L+++V +N + SF E DEI Y N EA I M L+ LK G S IGVI PY Q E+ + +++ ++E NT+DQ+QGRDK+++I S + IL DQRRL VAI+RAK+KLI++G++ L Q Y P +
Sbjct: 111 WNCMPMQVGDTVSLLGKWDP--SAGCYVVDKEQGYCVSHPDFLISGTTVTGSLFCKRKSVLQERFRGLD--SGNSMVIGTLVHELLQKVLRQKLFDKKHIQSALQEMLASSSLAHLLYASNLIQVEMEDHLMKFIDPIVSFVAQYVKGEPPSVLLPEVYRGQIHEICDIEENLWVPQLGLKGKVDVSVKVKNQRQREEIIPLELKTGRASFSMEHKGQLLLYQLMHSAQGR-DTQSGLLLYLKEGLLREVASGRNEQR-DLLMLRNDLAYWLTREVAIPASKEDPLEQLPLPEPVYHHSACGNCAYNTICSSFA---QKDSSLQLSDSHPLKKLMPQLLEHLSEADHAYVQHWCGLLALEEQHNRQSSHVRSFWTKDPAEREKEGIAIRHLKLVKGQEVILEEGRYRQTLELGEEADPSRDLSLSGFDLGEYVVISSTS--RLAVAAGFIVSIEARRLDLRLERDLSQRYSEETFI--------IDKHDSQSFATFNFTNLGMLLSEGERFQELRDIIVAKKPPEQHKVLPKIILTKGAPILLNLNKVQQNAALRALTTSSHLLIKGLPGTGKTQTLVALVRLLHLLGKSVLITAQTHSAVDNLIMRLLPFGLPMMRLGSGSRIHKQLEEISEERLT-----KDCKTVEELEKALGQPSIVGVTCLGCGHPLFQRRQFDYCIVDEATQVLQPTVLRPLIHC----SKFVLVGDPEQLPPIVRSKEARQRGADETLFQRL---DSEKATAVLSLQYRMNKTITRLANELTYGGDLKCASDEVSGARF-EVELLNEAPRWVQRALTTHLEQAVTLINTGDCLERCQEFVYASQRLVDTCSSIEQSFSEDKDEIRKLTSKKRVSKYTNYCEAGIVMHLLRYLLKSGYEASRIGVIAPYRAQVELFKKLASKLDTDLECNTVDQFQGRDKNLIIYSCSKTGGDFSDMERSREAEILEDQRRLTVAITRAKNKLILLGDIKCLEQ-YGPFR 1063
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Fly
Match: Upf1 (gene:FBgn0030354 transcript:FBtr0342744) HSP 1 Score: 129.028 bits (323), Expect = 9.734e-30 Identity = 132/443 (29.80%), Postives = 208/443 (46.95%), Query Frame = 1
Query: 2152 PDQRRVIMSAI---FSKDYLLIKGYPGSGKTSCIVAMIRIFV-LMGMKVLVSSYTHSAVSNILQKL------------DARE-----INFLYL---------NGNHSVCEKLKNRT--LSYILPEKKFENLFNF--HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQI---TCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
PD R + A+ + LI+G PG+GKT ++ V L G VLV + +++AV + +K+ +RE ++FL L N ++LK+ T LS EK++ NL + L+E DV+ TC+ A LSR+KF ++IDE+ Q T P ++P++ + IL GD QL P+V A GL QSLF +L P L++QYRM+ E+ + + Y + CA D+ L+F W + +R + FL Q + + N+ EA ++ +FLK G IG+I PY Q+ +++ M E+EI ++D +QGR+K I+I+S V ++ E LND RRLNVA++RAK +I++GN L++
Sbjct: 450 PDLNRSQVYAVKHALQRPLSLIQGPPGTGKTVTSATIVYQLVKLHGGTVLVCAPSNTAVDQLTEKIHRTNLKVVRVCAKSREAIDSPVSFLALHNQIRNMETNSELKKLQQLKDETGELSSA-DEKRYRNLKRAAENQLLEAADVICCTCVGAGDGRLSRVKFTSILIDESMQSTEPECMVPVVL---GAKQLILVGDHCQLGPVVMCKKAARAGLSQSLFERLVVLGIRP---FRLEVQYRMHPELSQFPSNFFYEGSLQNGVCAEDRRL-----------KLDFPW------PQPERPMFFLVTQGQEEIAGSGTSFLNRTEAANVEKITTRFLKAGIKPEQIGIITPYEGQRAYLVQYMQYQGSLHSRLYQEIEIASVDAFQGREKDIIIMSCV-------RSNERQGIGFLNDPRRLNVALTRAKFGIIIVGNPKVLAK 861
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Fly
Match: Upf1 (gene:FBgn0030354 transcript:FBtr0073596) HSP 1 Score: 129.028 bits (323), Expect = 9.734e-30 Identity = 132/443 (29.80%), Postives = 208/443 (46.95%), Query Frame = 1
Query: 2152 PDQRRVIMSAI---FSKDYLLIKGYPGSGKTSCIVAMIRIFV-LMGMKVLVSSYTHSAVSNILQKL------------DARE-----INFLYL---------NGNHSVCEKLKNRT--LSYILPEKKFENLFNF--HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQI---TCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
PD R + A+ + LI+G PG+GKT ++ V L G VLV + +++AV + +K+ +RE ++FL L N ++LK+ T LS EK++ NL + L+E DV+ TC+ A LSR+KF ++IDE+ Q T P ++P++ + IL GD QL P+V A GL QSLF +L P L++QYRM+ E+ + + Y + CA D+ L+F W + +R + FL Q + + N+ EA ++ +FLK G IG+I PY Q+ +++ M E+EI ++D +QGR+K I+I+S V ++ E LND RRLNVA++RAK +I++GN L++
Sbjct: 450 PDLNRSQVYAVKHALQRPLSLIQGPPGTGKTVTSATIVYQLVKLHGGTVLVCAPSNTAVDQLTEKIHRTNLKVVRVCAKSREAIDSPVSFLALHNQIRNMETNSELKKLQQLKDETGELSSA-DEKRYRNLKRAAENQLLEAADVICCTCVGAGDGRLSRVKFTSILIDESMQSTEPECMVPVVL---GAKQLILVGDHCQLGPVVMCKKAARAGLSQSLFERLVVLGIRP---FRLEVQYRMHPELSQFPSNFFYEGSLQNGVCAEDRRL-----------KLDFPW------PQPERPMFFLVTQGQEEIAGSGTSFLNRTEAANVEKITTRFLKAGIKPEQIGIITPYEGQRAYLVQYMQYQGSLHSRLYQEIEIASVDAFQGREKDIIIMSCV-------RSNERQGIGFLNDPRRLNVALTRAKFGIIIVGNPKVLAK 861
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Fly
Match: CG6967 (gene:FBgn0034187 transcript:FBtr0300276) HSP 1 Score: 74.7146 bits (182), Expect = 3.442e-13 Identity = 107/437 (24.49%), Postives = 193/437 (44.16%), Query Frame = 1
Query: 2161 RRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLM--GMKVLVSSYTHSAVSNILQKLDAREINFLYLNGN-----HSVCEKLKN------RTLSYILPEKKFENLFNF-----------HNLIENIDVVGVTCLSANHAALSRLKFDV---------VIIDEAGQITLPVSIMPLLKMDQNKG-KFILTGDLLQLPPLVRNSTARINGLDQSLFSKLC--------EFNHPDSICELKL--QYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSF-----VEADEIYFNKFEAKITMELVEQFLKIGAL-ESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGN 3309
+R++ Y+L G PG+GKT+ IV I L ++LV++ ++SA I KL Y+ N H +KL R S + EK F ++ + ++ I+ +V + A ++RL D + IDEAG T P +++ ++ + Q IL+GD QL +++++ A GL +SL +L E + D +++L YR + +I++L N + YN ++ A A M+ +N W S L + +Q + + +N EA++ V++ + + + D+G++ PY Q +++ + VE+ +++ YQGR+K+I+I S V +G + + RR+NV +SRAK LI++GN
Sbjct: 259 QRIVAGPSTQGPYILF-GPPGTGKTTTIVEAILQLRLQQPQSRILVTAGSNSACDTIALKLCE------YIESNIRLQEHFAQQKLPEPDHQLIRVYSRSIYEKGFASVPSLLLKNSNCSKSIYDHIKASRIVKYGIIVATLCTVARLVTDTLGRYNFFTHIFIDEAGASTEPEALIGIMGIKQTADCHVILSGDHKQLGAVIKSNRAASLGLSRSLMERLLQSDCYKSDENGNYDRNRQMRLCRNYRSHPQIVRLFNELYYNGELK------AQAPAMD---VNLAANW------SVLTNPQFPIIFQATHGVTNREQNSTSSYNNLEAEVICWYVKRLINDRVVGQEDVGIVAPYTAQGKLVTKLLQSKGYPNVEVGSVETYQGREKTIIIASLVKSFTNMG---------FMCNPRRVNVLLSRAKALLILVGN 664
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Zebrafish
Match: dna2 (DNA replication helicase/nuclease 2 [Source:ZFIN;Acc:ZDB-GENE-090313-71]) HSP 1 Score: 433.721 bits (1114), Expect = 3.017e-130 Identity = 326/1010 (32.28%), Postives = 494/1010 (48.91%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQV------------------------IDVEENIWSTKYGIKGKIDVTLQTN---------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT-----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQND--SDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNS--KKSIRNVTFESSSLSGINFL----IKFNLPQK----PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLI-CSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP-------EKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQC-------CSSELDRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
K LT+T + L WE T+V VGDI + S GLWTI+ L++ P L+S TS+ S+ C R+ VL F G S K M+ G+ +H IFQ+ +++ A + P+ L Y T ++ +E+E Y P I +W + L P+ + Q+ +D+EENIW ++G+KGKIDVT E +PLELKTG+ + S EH QV+LY LM S + + + G L+Y+K G+ + N IKIRN L N I SQ+ LP+++++ + C+ C C +++R + D CS+ + ++ + HL + ++F HW+ L LE L+ N+ +NI +Q + ++ I G++ L K + +K + GD I+VS D + + GYV E + + S +K L S T R+D + ++L L LM+ + ++LR+ IV +PP F ++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V + ILP K + L L +V TC+ H SR +FD I+DEA QI+ PV I PL +F+L GD QLPP+V+N+ AR G+D+SLF +L +H D++ +L +QYRMN I+ L N + Y ++ C +++ A A + ++ LE + CQ + E + V FL+ P VE I N EA + LV LK G DIGVI PY QQ ++I ++ + +E+NT+D+YQGRDKS++I+SFV + E N +L D RRLNVAI+RAKHKL+M+G+ L + Y P++ + L+ +IS
Sbjct: 381 KHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVS-GLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGS-KQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSAR-RCDPEAGFLVYLKTGSLHPVVG-NHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQ---LPAIVSDQQTCKYCPQKRNCAIYNRAVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALT-MENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEI-AVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHE--------ILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQ----RFVLVGDHQQLPPIVQNAEARSLGMDESLFKRL--EHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGIS-NHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNA------EGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTL-RRYAPLEKLLCHLQQEDMIS 1359
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Zebrafish
Match: dna2 (DNA replication helicase/nuclease 2 [Source:ZFIN;Acc:ZDB-GENE-090313-71]) HSP 1 Score: 433.335 bits (1113), Expect = 4.029e-130 Identity = 324/997 (32.50%), Postives = 488/997 (48.95%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQV------------------------IDVEENIWSTKYGIKGKIDVTLQTN---------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT-----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQND--SDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNS--KKSIRNVTFESSSLSGINFL----IKFNLPQK----PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLI-CSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP-------EKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSS-------ELDRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIK 3345
K LT+T + L WE T+V VGDI + S GLWTI+ L++ P L+S TS+ S+ C R+ VL F G S K M+ G+ +H IFQ+ +++ A + P+ L Y T ++ +E+E Y P I +W + L P+ + Q+ +D+EENIW ++G+KGKIDVT E +PLELKTG+ + S EH QV+LY LM S + + + G L+Y+K G+ + N IKIRN L N I SQ+ LP+++++ + C+ C C +++R + D CS+ + ++ + HL + ++F HW+ L LE L+ N+ +NI +Q + ++ I G++ L K + +K + GD I+VS D + + GYV E + + S +K L S T R+D + ++L L LM+ + ++LR+ IV +PP F ++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V + ILP K + L L +V TC+ H SR +FD I+DEA QI+ PV I PL +F+L GD QLPP+V+N+ AR G+D+SLF +L +H D++ +L +QYRMN I+ L N + Y ++ C +++ A A + ++ LE + CQ S E + V FL+ P VE I N EA + LV LK G DIGVI PY QQ ++I ++ + +E+NT+D+YQGRDKS++I+SFV + E N +L D RRLNVAI+RAKHKL+M+G+ L + Y P++
Sbjct: 381 KHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVS-GLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGS-KQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSAR-RCDPEAGFLVYLKTGSLHPVVG-NHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQ---LPAIVSDQQTCKYCPQKRNCAIYNRAVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALT-MENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEI-AVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHE--------ILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQ----RFVLVGDHQQLPPIVQNAEARSLGMDESLFKRL--EHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGIS-NHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNA------EGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTL-RRYAPLE 1346
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Zebrafish
Match: BX530068.2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:559535]) HSP 1 Score: 432.565 bits (1111), Expect = 6.571e-130 Identity = 327/1010 (32.38%), Postives = 494/1010 (48.91%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQV------------------------IDVEENIWSTKYGIKGKIDVTLQTN---------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT-----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQND--SDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNS--KKSIRNVTFESSSLSGINFL----IKFNLPQK----PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLI-CSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP-------EKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSS-------ELDRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
K LT+T + L WE T+V VGDI + S GLWTI+ L++ P L+S TS+ S+ C R+ VL F G S K M+ G+ +H IFQ+ +++ A + P+ L Y T ++ +E+E Y P I +W + L P+ + Q+ +D+EENIW ++G+KGKIDVT E +PLELKTG+ + S EH QV+LY LM S + + + G L+Y+K G+ + N IKIRN L N I SQ+ LP+++++ + C+ C C +++R + D CS+ + ++ + HL + ++F HW+ L LE L+ N+ +NI +Q + ++ I G++ L K + +K + GD I+VS D + + GYV E + + S +K L S T R+D + ++L L LM+ + ++LR+ IV +PP F ++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V + ILP K + L L +V TC+ H SR +FD I+DEA QI+ PV I PL +F+L GD QLPP+V+N+ AR G+D+SLF +L +H D++ +L +QYRMN I+ L N + Y ++ C +++ A A + ++ LE + CQ S E + V FL+ P VE I N EA + LV LK G DIGVI PY QQ ++I ++ + +E+NT+D+YQGRDKS++I+SFV + E N +L D RRLNVAI+RAKHKL+M+G+ L + Y P++ + L+ +IS
Sbjct: 378 KHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVS-GLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGS-KQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSAR-RCDPEAGFLVYLKTGSLHPVVG-NHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQ---LPAIVSDQQTCKYCPQKRNCAIYNRSVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALT-MENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEI-AVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHE--------ILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQ----RFVLVGDHQQLPPIVQNAEARSLGMDESLFKRL--EHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGIS-NHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNA------EGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTL-RRYAPLEKLLCHLQQEDMIS 1356
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Zebrafish
Match: BX530068.2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:559535]) HSP 1 Score: 432.18 bits (1110), Expect = 8.271e-130 Identity = 327/1010 (32.38%), Postives = 494/1010 (48.91%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQV------------------------IDVEENIWSTKYGIKGKIDVTLQTN---------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT-----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQND--SDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNS--KKSIRNVTFESSSLSGINFL----IKFNLPQK----PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLI-CSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP-------EKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSS-------ELDRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
K LT+T + L WE T+V VGDI + S GLWTI+ L++ P L+S TS+ S+ C R+ VL F G S K M+ G+ +H IFQ+ +++ A + P+ L Y T ++ +E+E Y P I +W + L P+ + Q+ +D+EENIW ++G+KGKIDVT E +PLELKTG+ + S EH QV+LY LM S + + + G L+Y+K G+ + N IKIRN L N I SQ+ LP+++++ + C+ C C +++R + D CS+ + ++ + HL + ++F HW+ L LE L+ N+ +NI +Q + ++ I G++ L K + +K + GD I+VS D + + GYV E + + S +K L S T R+D + ++L L LM+ + ++LR+ IV +PP F ++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V + ILP K + L L +V TC+ H SR +FD I+DEA QI+ PV I PL +F+L GD QLPP+V+N+ AR G+D+SLF +L +H D++ +L +QYRMN I+ L N + Y ++ C +++ A A + ++ LE + CQ S E + V FL+ P VE I N EA + LV LK G DIGVI PY QQ ++I ++ + +E+NT+D+YQGRDKS++I+SFV + E N +L D RRLNVAI+RAKHKL+M+G+ L + Y P++ + L+ +IS
Sbjct: 378 KHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVS-GLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGS-KQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSAR-RCDPEAGFLVYLKTGSLHPVVG-NHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQ---LPAIVSDQQTCKYCPQKRNCAIYNRSVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALT-MENKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEI-AVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVSNILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHE--------ILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQ----RFVLVGDHQQLPPIVQNAEARSLGMDESLFKRL--EHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVPAPETVEKSGIS-NHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNA------EGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTL-RRYAPLEKLLCHLQQEDMIS 1356
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Zebrafish
Match: BX890597.2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:559535]) HSP 1 Score: 431.024 bits (1107), Expect = 2.532e-129 Identity = 321/1009 (31.81%), Postives = 492/1009 (48.76%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQV------------------------IDVEENIWSTKYGIKGKIDVTLQTN---------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT-----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQND--SDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNS--KKSIRNVTFESSSLSGINFL----IKFNLPQK----PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLI-CSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP-------EKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYF----------NKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
K LT+T + L WE T+V VGDI + S GLWTI+ L++ P L+S TS+ S+ C R+ VL F G S K M+ G+ +H IFQ+ +++ A + P+ L Y T ++ +E+E Y P I +W + L P+ + Q+ +D+EENIW ++G+KGKIDVT E +PLELKTG+ + S EH QV+LY LM S + + + G L+Y+K G+ + N IKIRN L N I SQ+ LP+++++ + C+ C C +++R + D CS+ + ++ + HL + ++F HW+ L LE L+ + +NI +Q + ++ I G++ L K + +K + GD I+VS D + + GYV E + + S +K L S T R+D + ++L L LM+ + ++LR+ IV +PP F ++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V + ILP K + L L +V TC+ H SR +FD I+DEA QI+ PV I PL +F+L GD QLPP+V+N+ AR G+D+SLF +L +H D++ +L +QYRMN I+ L N + Y ++ C +++ A A + ++ LE + CQ S ++ + N F++ E+ N EA + LV LK G DIGVI PY QQ ++I ++ + +E+NT+D+YQGRDKS++I+SFV + E N +L D RRLNVAI+RAKHKL+M+G+ L + Y P++ + L+ +IS
Sbjct: 378 KHLTITCSKTSQATETCILKDGWESTLVTVGDIIHLEGKCVS-GLWTIDRDSAFLILLPDLLISGTSIASSIRCMRRGVLGEMFKAFDGGS-KQMLNGTIVHDIFQKAAMSSDFSSEKIQTFATEALRSPNYLGQMYSLKLTQADMRQEVEEYLPSISEWAKQYLHTSPQAGQKQLTLKLPSDGALSKQDASSSVMVTDFVDIEENIWCPRFGLKGKIDVTAGVKIHRRGRKPMERIVPLELKTGKESNSIEHRSQVILYTLMSSAR-RCDPEAGFLVYLKTGSLHPVVG-NHMDRRELIKIRNSLAHHIGNNLIKVDGKSQMAQ---LPAIVSDQQTCKYCPQKRNCAIYNRSVERDPVEKCSENPQAFVQSESEHLSQVHLQYFSHWLLLCSLEALT-MEKKGGRRNIWLQTAQQREKIGGCAGNMRLAGGVSILSDGVYKHSFERKEGEQTLTGLIVGDRIVVSDQDSVLIGLSTGYVTEI-AVNRVTCSLDKNLSKYSSNTVFRLDQDEGAGGLSTHLGNLSMLMENTPTSERLRELIVEFRPPQFIDSLSSVLPRDAKDIVANILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFKIGFLRLGRPQKVHHE--------ILPFTEEACRAKGIQTLEELEQLYSRELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCIGPLFYAQ----RFVLVGDHQQLPPIVQNAEARSLGMDESLFKRL--EHHRDAVVQLNVQYRMNSAIMSLSNALMYEGRLECGSEKTANAVLQLPSRAQAEEELELYVCQPQYSAWVQAALEPNSPVCFLDTSEVAAPETVEKSGISNHTEAVLVQGLVTLLLKAGCRACDIGVIAPYRQQLKVISSLLQGDAFKALEVNTVDKYQGRDKSVIIVSFVRSNA------EGNLGELLQDWRRLNVAITRAKHKLLMLGSAPTL-RRYAPLEKLLCHLQQEDMIS 1356
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Xenopus
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:100170166]) HSP 1 Score: 403.29 bits (1035), Expect = 6.625e-122 Identity = 310/943 (32.87%), Postives = 483/943 (51.22%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKW--------INRNLSKKPEISKMQVIDVEENIWSTKYGIKGKIDVTLQ--------TNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFT------SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDC--SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSK------KSIRNV--TFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS-----ELDRSVIFLNYQ--PSF-VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN----MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
W + GDI + + WTI+ G L++ P L+S TS+ + C R+ VL F V + ++ M+IG+ +H IFQ D + ++E+A P L Y+ +++ E++ Y P KW +N++ ++S + +D+EENIWS ++G+KGKIDVT + ++ +PLELKTG+ + S EH QV+LY L+ ++ E + + GLLLY+K G + N +KIRNEL+ ++ S ++T A LP++I + + C+ C + C ++SR + Q +C M +++ T HL + ++F W + LE S+ S KNI + +S + I N+ T ++S L F P + GD ++VS +D K +A+ GY+ E +I I + R+D S+L L LM+ S + KLR I+ P F H++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL ++ FL L + ++ + I K ++L L + VV TC+ NH +R +FD I+DEA QI+ P+ + PL D +F+L GD QLPPLV+++ AR G+ +SLF +L E N +++ +L +QYRMN +I+ L N + Y ++ CA+D+V+ A + + H + LEF + Q E V FLN + P+ E N EAK+ L + FLK G SDIG+I PY QQ ++I N +S VE+NT+D+YQGRDKS++I+SFV + + ++L D RRLNVA++RAKHKLIM+G + LS+
Sbjct: 79 WVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFK-VCDKGSRQMLIGTMLHDIFQRATTRGFTD-SVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINATKVS--EFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGLLLYLKTG-NMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLAS-LPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGR-KNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGED-KFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFAD----RFVLVGDHQQLPPLVQSAEARELGMSESLFKRL-ERNQ-EAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNI------DGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSR 1000
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Xenopus
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:100170166]) HSP 1 Score: 402.905 bits (1034), Expect = 1.937e-121 Identity = 312/952 (32.77%), Postives = 484/952 (50.84%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKW---------------INRNLSKKP-EISKM-QVIDVEENIWSTKYGIKGKIDVTLQ--------TNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFT------SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDC--SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSK------KSIRNV--TFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS-----ELDRSVIFLNYQ--PSF-VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN----MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
W + GDI + + WTI+ G L++ P L+S TS+ + C R+ VL F V + ++ M+IG+ +H IFQ D + ++E+A P L Y+ +++ E++ Y P KW + R + P E +K+ + +D+EENIWS ++G+KGKIDVT + ++ +PLELKTG+ + S EH QV+LY L+ ++ E + + GLLLY+K G + N +KIRNEL+ ++ S ++T A LP++I + + C+ C + C ++SR + Q +C M +++ T HL + ++F W + LE S+ S KNI + +S + I N+ T ++S L F P + GD ++VS +D K +A+ GY+ E +I I + R+D S+L L LM+ S + KLR I+ P F H++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL ++ FL L + ++ + I K ++L L + VV TC+ NH +R +FD I+DEA QI+ P+ + PL D +F+L GD QLPPLV+++ AR G+ +SLF +L E N +++ +L +QYRMN +I+ L N + Y ++ CA+D+V+ A + + H + LEF + Q E V FLN + P+ E N EAK+ L + FLK G SDIG+I PY QQ ++I N +S VE+NT+D+YQGRDKS++I+SFV + + ++L D RRLNVA++RAKHKLIM+G + LS+
Sbjct: 91 WVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFK-VCDKGSRQMLIGTMLHDIFQRATTRGFTD-SVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINVTRPTAGDPTEATKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGLLLYLKTG-NMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLAS-LPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGR-KNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGED-KFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFAD----RFVLVGDHQQLPPLVQSAEARELGMSESLFKRL-ERNQ-EAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNI------DGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSR 1023
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Xenopus
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:100170166]) HSP 1 Score: 402.134 bits (1032), Expect = 2.256e-121 Identity = 310/951 (32.60%), Postives = 483/951 (50.79%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKW---------------INRNLSKKPEISKM-QVIDVEENIWSTKYGIKGKIDVTLQ--------TNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFT------SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDC--SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSK------KSIRNV--TFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS-----ELDRSVIFLNYQ--PSF-VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN----MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
W + GDI + + WTI+ G L++ P L+S TS+ + C R+ VL F V + ++ M+IG+ +H IFQ D + ++E+A P L Y+ +++ E++ Y P KW + R+ + +K+ + +D+EENIWS ++G+KGKIDVT + ++ +PLELKTG+ + S EH QV+LY L+ ++ E + + GLLLY+K G + N +KIRNEL+ ++ S ++T A LP++I + + C+ C + C ++SR + Q +C M +++ T HL + ++F W + LE S+ S KNI + +S + I N+ T ++S L F P + GD ++VS +D K +A+ GY+ E +I I + R+D S+L L LM+ S + KLR I+ P F H++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL ++ FL L + ++ + I K ++L L + VV TC+ NH +R +FD I+DEA QI+ P+ + PL D +F+L GD QLPPLV+++ AR G+ +SLF +L E N +++ +L +QYRMN +I+ L N + Y ++ CA+D+V+ A + + H + LEF + Q E V FLN + P+ E N EAK+ L + FLK G SDIG+I PY QQ ++I N +S VE+NT+D+YQGRDKS++I+SFV + + ++L D RRLNVA++RAKHKLIM+G + LS+
Sbjct: 79 WVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFK-VCDKGSRQMLIGTMLHDIFQRATTRGFTD-SVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINVTRDGGNESGATKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGLLLYLKTG-NMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLAS-LPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGR-KNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGED-KFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFAD----RFVLVGDHQQLPPLVQSAEARELGMSESLFKRL-ERNQ-EAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNI------DGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSR 1010
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Xenopus
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:100170166]) HSP 1 Score: 402.905 bits (1034), Expect = 5.414e-121 Identity = 312/952 (32.77%), Postives = 484/952 (50.84%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKW---------------INRNLSKKP-EISKM-QVIDVEENIWSTKYGIKGKIDVTLQ--------TNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFT------SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDC--SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSK------KSIRNV--TFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS-----ELDRSVIFLNYQ--PSF-VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN----MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
W + GDI + + WTI+ G L++ P L+S TS+ + C R+ VL F V + ++ M+IG+ +H IFQ D + ++E+A P L Y+ +++ E++ Y P KW + R + P E +K+ + +D+EENIWS ++G+KGKIDVT + ++ +PLELKTG+ + S EH QV+LY L+ ++ E + + GLLLY+K G + N +KIRNEL+ ++ S ++T A LP++I + + C+ C + C ++SR + Q +C M +++ T HL + ++F W + LE S+ S KNI + +S + I N+ T ++S L F P + GD ++VS +D K +A+ GY+ E +I I + R+D S+L L LM+ S + KLR I+ P F H++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL ++ FL L + ++ + I K ++L L + VV TC+ NH +R +FD I+DEA QI+ P+ + PL D +F+L GD QLPPLV+++ AR G+ +SLF +L E N +++ +L +QYRMN +I+ L N + Y ++ CA+D+V+ A + + H + LEF + Q E V FLN + P+ E N EAK+ L + FLK G SDIG+I PY QQ ++I N +S VE+NT+D+YQGRDKS++I+SFV + + ++L D RRLNVA++RAKHKLIM+G + LS+
Sbjct: 147 WVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFK-VCDKGSRQMLIGTMLHDIFQRATTRGFTD-SVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINVTRPTAGDPTEATKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGLLLYLKTG-NMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLAS-LPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGR-KNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGED-KFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFAD----RFVLVGDHQQLPPLVQSAEARELGMSESLFKRL-ERNQ-EAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFCLTKLFLKAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNI------DGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSR 1079
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Xenopus
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:100170166]) HSP 1 Score: 384.8 bits (987), Expect = 1.597e-115 Identity = 306/944 (32.42%), Postives = 479/944 (50.74%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKW--------INR---NLSKKP-EISKMQVI-----DVEENIWSTKYGIKGKIDVTLQ--------TNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFT------SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDC--SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSK------KSIRNV--TFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN----MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
W + GDI + + WTI+ G L++ P L+S TS+ + C R+ VL F V + ++ M+IG+ +H IFQ D + ++E+A P L Y+ +++ E++ Y P KW +N+ N+ K P ++K +I D+EENIWS ++G+KGKIDVT + ++ +PLELKTG+ + S EH QV+LY L+ ++ E + + GLLLY+K G + N +KIRNEL+ ++ S ++T A LP++I + + C+ C + C ++SR + Q +C M +++ T HL + ++F W + LE S+ S KNI + +S + I N+ T ++S L F P + GD ++VS +D K +A+ GY+ E +I I + R+D S+L L LM+ S + KLR I+ P F H++ L K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL ++ FL L + ++ + I K ++L L + VV TC+ NH +R +FD I+DEA QI+ P+ + PL D +F+L GD QLPPLV+++ AR G+ +SLF +L E N +++ +L +QYRMN +I+ L N + Y ++ CA+D+V+ A + + H + LEF + Q S + V+ +PS + + F E K G SDIG+I PY QQ ++I N +S VE+NT+D+YQGRDKS++I+SFV + + ++L D RRLNVA++RAKHKLIM+G + LS+
Sbjct: 55 WVALHIKPGDIIHLEGNCAFDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFK-VCDKGSRQMLIGTMLHDIFQRATTRGFTD-SVLQELAHHTVHGPKYLKEMYQLKLNQTDVMGEVQEYLPSFAKWAIDFMTHPLNQHQINIPKMPFPLNKTGIIFSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKSHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGLLLYLKTG-NMYSVPGNRLDRRELLKIRNELSYYLTNVVHKSDNGSKEITLAS-LPALIADRQACKFCSQMRNCALYSRSVEQQIENCYIPAEMIPVVQKETEHLNKDHLQYFRLWYLMCTLEGNSKDSKMGR-KNIWMMSSSEREEDGQCIGNLIRTGPVQTISDGQHLHSFQRKSGTVPATNLMSGDRVVVSGED-KFLALSSGYIKEVKHNNITCILDRSLGKLPEDLLFRLDHEEGGGGLESHLGNLSRLMENSPVSDKLRKLIIDFSKPNFVQHLSSVLPSDAKDIVANILKGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGYSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFAD----RFVLVGDHQQLPPLVQSAEARELGMSESLFKRL-ERNQ-EAVVQLTVQYRMNSQIMALSNKLVYEGRLECASDRVSNAVVKLPHIKTLLLELEFRESQ--ESMWIKDVL----EPS----NPVCFLNTE------------KAGCRPSDIGIIAPYRQQLKVISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNI------DGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLSR 957
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Mouse
Match: Dna2 (DNA replication helicase/nuclease 2 [Source:MGI Symbol;Acc:MGI:2443732]) HSP 1 Score: 421.394 bits (1082), Expect = 2.518e-128 Identity = 325/1010 (32.18%), Postives = 503/1010 (49.80%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECC-------RLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKP--EISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADC----LPSVITNIRLCQSCEYLSLCTMFSRLSQ---NDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNII-------VQNSKKSIRNV--TFESSSLSGINFLIKF---NLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSS------ELDRSVIFLNY----QPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFN-ILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQII 3381
K+L VT + + +V L W V GDI + S W I++ G ++ P ++S TSV S+ C R+ VL F G S + + M+IG+ +H +FQ+ RL+ L L +RE+ L Y + +EIL E+E Y P KW + K P E +MQ+ +D+EE+IWS ++G+KGKIDVT+ + +PLELKTG+ + S EH QV+LY L+ ++ E + + G LLY+K G + + N +K+RN L +L S + LP +I + C+ C + C ++SR + +D+ +AM I++ T HL ++F W ++ LE S+S + I ++ S + N+ T S + +L F N P P + GD II+S ++ K A+ GYV + + + T R+D + + P+ NLS LM+ ++ ++LR+ I+ + P F +++ L K +I+K N QR+ + + SKDY LI G PG+GKT+ I A++RI G VL++SYTHSAV NIL KL ++ FL L +H V ++ T I + +L + L + +V TC+ NH SR FD I+DEA QI+ PV + PL +F+L GD QLPPLV N AR G+ +SLF +L E N ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A + ++ SL+ + S E D V FLN P VE + N EA++ + L F+K G SDIGVI PY QQ +I ++ S VE+NT+D+YQGRDKS++++SFV ++ E+ + +L D RRLNVA++RAKHKLI++G++ L + + P+ + D L A Q+I
Sbjct: 65 KRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEP-WIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRG-SDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREV--------RHLKEMYRLNLSQDEILCEVEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGWLLYLKTG-QMYPVPANHLDKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLE--SQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPM-PATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTVFRLDREERHGDISTPLGNLS---KLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLF----FSRRFVLVGDHQQLPPLVVNREARALGMSESLFKRL-ERNE-SAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVANAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENGGVS-NVTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLLARSSVGMVEVNTVDKYQGRDKSLILVSFV-------RSNEDGTLGELLKDWRRLNVALTRAKHKLILLGSVSSL-KRFPPLGTLFDHLNAEQLI 1041
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Mouse
Match: Dna2 (DNA replication helicase/nuclease 2 [Source:MGI Symbol;Acc:MGI:2443732]) HSP 1 Score: 350.517 bits (898), Expect = 2.871e-103 Identity = 274/897 (30.55%), Postives = 428/897 (47.71%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECC-------RLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKP--EISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADC----LPSVITNIRLCQSCEYLSLCTMFSRLSQ---NDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNII-------VQNSKKSIRNV--TFESSSLSGINFLIKF---NLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSS------ELDRSVIFLNY----QPSFVEADEIYFNKFEAKITMELVEQFLKIGALES 3057
K+L VT + + +V L W V GDI + S W I++ G ++ P ++S TSV S+ C R+ VL F G S + + M+IG+ +H +FQ+ RL+ L L +RE+ L Y + +EIL E+E Y P KW + K P E +MQ+ +D+EE+IWS ++G+KGKIDVT+ + +PLELKTG+ + S EH QV+LY L+ ++ E + + G LLY+K G + + N +K+RN L +L S + LP +I + C+ C + C ++SR + +D+ +AM I++ T HL ++F W ++ LE S+S + I ++ S + N+ T S + +L F N P P + GD II+S ++ K A+ GYV + + + T R+D + + P+ NLS LM+ ++ ++LR+ I+ + P F +++ L K +I+K N QR+ + + SKDY LI G PG+GKT+ I A++RI G VL++SYTHSAV NIL KL ++ FL L +H V ++ T I + +L + L + +V TC+ NH SR FD I+DEA QI+ PV + PL +F+L GD QLPPLV N AR G+ +SLF +L E N ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A + ++ SL+ + S E D V FLN P VE + N EA++ + L F+K A ++
Sbjct: 65 KRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEP-WIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRG-SDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREV--------RHLKEMYRLNLSQDEILCEVEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGWLLYLKTG-QMYPVPANHLDKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLE--SQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPM-PATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTVFRLDREERHGDISTPLGNLS---KLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLF----FSRRFVLVGDHQQLPPLVVNREARALGMSESLFKRL-ERNE-SAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVANAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENGGVS-NVTEARLIVFLTSTFIKAAAPQT 936
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Mouse
Match: Upf1 (UPF1 regulator of nonsense transcripts homolog (yeast) [Source:MGI Symbol;Acc:MGI:107995]) HSP 1 Score: 123.635 bits (309), Expect = 6.519e-28 Identity = 155/584 (26.54%), Postives = 258/584 (44.18%), Query Frame = 1
Query: 1759 LSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLK-IPISNLSVLGSLMDKSEIGQKLRDFIVHCKPP-------LFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMG-MKVLVSSYTHSAVSNILQKL------------DARE-----INFLYLNGN----HSVCE-----KLKNRT--LSYILPEKKFENLFNF--HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQI---TCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPI 3342
+ G +++ P +K + G I V + G ++AI L V +P + + + + K T + S+LK + SV G + K +G ++ D ++ C+ P L D + ++ AVK + + LI+G PG+GKT ++ G VLV + ++ AV + +K+ +RE ++FL L+ S+ E +LK+ T LS EK++ L L+ N DV+ TC+ A L++++F ++IDE+ Q T P ++P++ + IL GD QL P+V A GL QSLF +L P L++QYRM+ + + I Y + A D+V +F W + D+ + F Q + Y N+ EA ++ + LK GA IG+I PY Q+ +++ M EVEI ++D +QGR+K +I+S V +A E+ LND RRLNVA++RA++ +I++GN LS+ QP+
Sbjct: 352 MQGDEICLRYKGDLAPLWKGI-GHVIKVPDNYGDEIAIELRSSVGAP---VEVTHNFQVDFVWKSTSFD-RMQSALKTFAVDETSVSGYIYHKL-LGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKT------------------VLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSA-DEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVL---GAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRP---IRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVK----------KGFDFQW------PQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCV-------RANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSK--QPL 879
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Mouse
Match: Upf1 (UPF1 regulator of nonsense transcripts homolog (yeast) [Source:MGI Symbol;Acc:MGI:107995]) HSP 1 Score: 123.25 bits (308), Expect = 9.334e-28 Identity = 155/584 (26.54%), Postives = 258/584 (44.18%), Query Frame = 1
Query: 1759 LSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLK-IPISNLSVLGSLMDKSEIGQKLRDFIVHCKPP-------LFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMG-MKVLVSSYTHSAVSNILQKL------------DARE-----INFLYLNGN----HSVCE-----KLKNRT--LSYILPEKKFENLFNF--HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQI---TCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPI 3342
+ G +++ P +K + G I V + G ++AI L V +P + + + + K T + S+LK + SV G + K +G ++ D ++ C+ P L D + ++ AVK + + LI+G PG+GKT ++ G VLV + ++ AV + +K+ +RE ++FL L+ S+ E +LK+ T LS EK++ L L+ N DV+ TC+ A L++++F ++IDE+ Q T P ++P++ + IL GD QL P+V A GL QSLF +L P L++QYRM+ + + I Y + A D+V +F W + D+ + F Q + Y N+ EA ++ + LK GA IG+I PY Q+ +++ M EVEI ++D +QGR+K +I+S V +A E+ LND RRLNVA++RA++ +I++GN LS+ QP+
Sbjct: 363 MQGDEICLRYKGDLAPLWKGI-GHVIKVPDNYGDEIAIELRSSVGAP---VEVTHNFQVDFVWKSTSFD-RMQSALKTFAVDETSVSGYIYHKL-LGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKT------------------VLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSA-DEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVL---GAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRP---IRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVK----------KGFDFQW------PQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCV-------RANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSK--QPL 890
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Mouse
Match: Zgrf1 (zinc finger, GRF-type containing 1 [Source:MGI Symbol;Acc:MGI:1918893]) HSP 1 Score: 114.39 bits (285), Expect = 6.571e-25 Identity = 93/308 (30.19%), Postives = 147/308 (47.73%), Query Frame = 1
Query: 2464 LIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNS-TARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSF-VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM---------STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIH 3354
L++ + VVGVTC + L+ LKF VV++DE Q+T P S++P+ + K IL GD QLPP ++ S A NGL+Q+LF +LC H + L+ QYR + I + N + Y + I E E LE+ C F N + VE + + N EA T++L++ + G IGVI Y Q I N+ V+++T+D +QG +K I I+S V ++ ++R+NVA++R + L+++G+L L +N +VI
Sbjct: 1540 LLKQVRVVGVTCAACAFPCLNDLKFPVVVLDECSQMTEPASLLPIARFQCE--KLILVGDPKQLPPTIQGSDAAHENGLEQTLFDRLCLMGHKPVL--LRTQYRCHPAISAIANDLFYEGSLVNG--------ISERERSPVLEWLPTLC----------FYNVTGAEQVERENSFVNVAEATFTLKLIQSLMASGVESCMIGVITLYKSQMYKICNLLSAVDVGHPDVKAVQVSTVDAFQGAEKEITILSCV----------RTRQVGFIDSEKRMNVALTRGRRHLLIVGSLSCLRKNRLWGRVIQ 1815
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. UniProt/SwissProt
Match: sp|Q6ZQJ5|DNA2_MOUSE (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Mus musculus OX=10090 GN=Dna2 PE=1 SV=2) HSP 1 Score: 421.394 bits (1082), Expect = 1.762e-127 Identity = 325/1010 (32.18%), Postives = 503/1010 (49.80%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECC-------RLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKP--EISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADC----LPSVITNIRLCQSCEYLSLCTMFSRLSQ---NDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNII-------VQNSKKSIRNV--TFESSSLSGINFLIKF---NLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQCCSS------ELDRSVIFLNY----QPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFN-ILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQII 3381
K+L VT + + +V L W V GDI + S W I++ G ++ P ++S TSV S+ C R+ VL F G S + + M+IG+ +H +FQ+ RL+ L L +RE+ L Y + +EIL E+E Y P KW + K P E +MQ+ +D+EE+IWS ++G+KGKIDVT+ + +PLELKTG+ + S EH QV+LY L+ ++ E + + G LLY+K G + + N +K+RN L +L S + LP +I + C+ C + C ++SR + +D+ +AM I++ T HL ++F W ++ LE S+S + I ++ S + N+ T S + +L F N P P + GD II+S ++ K A+ GYV + + + T R+D + + P+ NLS LM+ ++ ++LR+ I+ + P F +++ L K +I+K N QR+ + + SKDY LI G PG+GKT+ I A++RI G VL++SYTHSAV NIL KL ++ FL L +H V ++ T I + +L + L + +V TC+ NH SR FD I+DEA QI+ PV + PL +F+L GD QLPPLV N AR G+ +SLF +L E N ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A + ++ SL+ + S E D V FLN P VE + N EA++ + L F+K G SDIGVI PY QQ +I ++ S VE+NT+D+YQGRDKS++++SFV ++ E+ + +L D RRLNVA++RAKHKLI++G++ L + + P+ + D L A Q+I
Sbjct: 65 KRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEP-WIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRG-SDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREV--------RHLKEMYRLNLSQDEILCEVEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGWLLYLKTG-QMYPVPANHLDKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLE--SQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPM-PATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTVFRLDREERHGDISTPLGNLS---KLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLNKPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLF----FSRRFVLVGDHQQLPPLVVNREARALGMSESLFKRL-ERNE-SAVVQLTVQYRMNRKIMSLSNKLTYAGKLECGSDRVANAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENGGVS-NVTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLLARSSVGMVEVNTVDKYQGRDKSLILVSFV-------RSNEDGTLGELLKDWRRLNVALTRAKHKLILLGSVSSL-KRFPPLGTLFDHLNAEQLI 1041
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. UniProt/SwissProt
Match: sp|D3ZG52|DNA2_RAT (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Rattus norvegicus OX=10116 GN=Dna2 PE=3 SV=1) HSP 1 Score: 420.624 bits (1080), Expect = 3.524e-127 Identity = 319/1001 (31.87%), Postives = 512/1001 (51.15%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADC----LPSVITNIRLCQSCEYLSLCTMFSRLSQ--NDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNEN-------PIKNIIVQNSKKSIRNV--TFESSSLSGINFLIKF---NLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYV--VESPGTDILIISSEKPLICSKMTKL-RIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEHESINSLEFWK------CQCCSSELDRSVIFLNY----QPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFN-ILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
K L +T+ + +V L W V GDI I S W +++ G +++P L+S TSV S+ C R+ VL F VS + + M+IG+ +H +FQ+ E +++E+A + ++ L Y + +E+ E+E Y P KW + + K K E +M + +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH QV+LY L+ ++ E + + G LLY+K G + + N +K+RN+L +L S + LP +I + C+ C + C ++SR + +D+ + M+ I++ T HL ++F W ++ LE S+ + ++ P + + S I ++ T + ++L F N P P + GD II+S ++ K A+ GYV +++ L+ + L + + +L R + + + P+ NLS LM+ ++ ++LR+ I+ K P F +++ L K +I+K N QR+ + + SKDY LI G PG+GKT+ I A++RI G VL++SYTHSAV NIL KL +I FL L +H V ++ T + + +L + L + VV TC+ +H SR FD I+DEA QI+ P+ + PL +F+L GD QLPPLV N AR G+ +SLF +L E N ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A I + + LEF+ E D V FLN P +E + N EA++ + L F+K G SDIG+I PY QQ I ++ S VE+NT+D+YQGRDKS++++SFV ++ E+ + +L D RRLNVAI+RAKHKLI++G++ L + + P++ + D L A Q+IS
Sbjct: 65 KHLLITVSQEGEQEVLCILRNGWSSVPVEPGDIIHIEGDCTSEP-WIVDDDFGYFILSPDMLISGTSVASSIRCLRRAVLSETFR-VSDTATRQMLIGTILHEVFQKAIS-ESFAPEKLQELALQTLREVRHLKEMYRLNLSQDEVRCEVEEYLPSFSKWADEFMHKGTKAEFPQMHLSLPSDSSDRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKTKYKIMPLELKTGKESNSIEHRGQVILYTLLSQERRE-DPEAGWLLYLKTG-QMYPVPANHLDKRELLKLRNQLAFSLLHRVSRAAAGEEARLLALPQIIEEEKTCKYCSQMGNCALYSRAVEQVHDTSIPEGMRSKIQEGTQHLTRAHLKYFSLWCLMLTLESQSKDTKKSHQSIWLTPASKL--EESGNCIGSLVRTEPVKRVCDGHYLHNFQRKNGPM-PATNLMAGDRIILSGEERKLFALSKGYVKRIDTAAVTCLLDRNLSTLPETTLFRLDREEKHGDINTPLGNLS---KLMENTDSSKRLRELIIDFKEPQFIAYLSSVLPHDAKDTVANILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQSHKVHPDIQKFTEEEMCRLRSIASLAHLEELYNSHPVVATTCMGISHPMFSRKTFDFCIVDEASQISQPICLGPLF----FSRRFVLVGDHKQLPPLVLNREARALGMSESLFKRL-ERNE-SAVVQLTIQYRMNRKIMSLSNKLTYEGKLECGSDRVANAVITLPNLKDVRLEFYADYSDNPWLAGVFEPDNPVCFLNTDKVPAPEQIENGGVS-NVTEARLIVFLTSTFIKAGCSPSDIGIIAPYRQQLRTITDLLARSSVGMVEVNTVDKYQGRDKSLILVSFV-------RSNEDGTLGELLKDWRRLNVAITRAKHKLILLGSVSSL-KRFPPLEKLFDHLNAEQLIS 1039
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. UniProt/SwissProt
Match: sp|Q8QHA5|DNA2_XENLA (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Xenopus laevis OX=8355 GN=dna2 PE=1 SV=1) HSP 1 Score: 408.297 bits (1048), Expect = 9.547e-123 Identity = 310/954 (32.49%), Postives = 485/954 (50.84%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKW-------------INRNLSKK---PEISKM-QVIDVEENIWSTKYGIKGKIDVTLQ--------TNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKF-----TSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDC--SQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKS------IRNV--TFESSSLSGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS-----ELDRSVIFLNYQ--PSF-VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRN----MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
W + GDI + + WTI+ G L++ P L+S TS+ + C R+ VL F V + ++ M+ G+ +H IFQ D + ++E+A P L Y+ +++ EI+ Y P + KW INR S E +K+ + +D+EENIWS ++G+KGKIDVT + + +PLELKTG+ + S EH QV+LY L+ ++ E + + GLLLY+K G + N +KIRNEL+ ++ S+ T LP++I + + C+ C + C +++R + Q +C M +++ T HL E ++F W + LE S+ S KNI + +S + I N+ T ++S + +L F P GD ++VS ++ + +A+ GY+ E +I I + + R+D L+ + NLS LM+ S + +KLR I+ P F H++ L K I++ N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL ++ FL L + ++ + I K ++L L + VV TC+ NH +R +FD I+DEA QI+ P+ + PL D +F+L GD QLPPLV+++ AR G+ +SLF +L E N +++ +L +QYRMN +I+ L N + Y ++ CA+D+V+ A + + H + LEF + Q E V FLN + P+ E N EAK+ L + +LK G SDIG+I PY QQ +MI N +S VE+NT+D+YQGRDKS++I+SFV + + ++L D RRLNVA++RAKHKLIM+G + L++
Sbjct: 91 WVALQIKPGDIIHLEGNCSVDNTWTISRDTGYLILYPDLLISGTSIANGIRCLRRSVLSEKFK-VCDKGSRQMLNGTMLHDIFQRATTCGFTD-SVLQELAHHTVHGPKYLKEMYQLKLNQADVMGEIQEYLPSLSKWATDFMTHPLNQQQINRTKSTAGDPTETTKVSEFLDIEENIWSPRFGLKGKIDVTARVKIHQKSKAHLKIMPLELKTGKESNSIEHRSQVVLYTLLSQERRE-DPEAGLLLYLKTG-NMYTVPGNRLDRRELLKIRNELSYYLTNVLHKSDNGSKETTLASLPAMIADRQACKYCSQMRNCALYNRSVEQQTENCYIPPEMIPVVQKETEHLTEDHLQYFRLWYLMCTLEANSKDSKMGR-KNIWMMSSSEREEDGQCIGNLIRTGHVQTISDVQYLHSFQRRSGSVPATNLASGDRVVVSGEE-RFLALSTGYIKEVKDENITCILDRSLVKLPEDLLFRLDHEEGGGGLEFHLGNLS---RLMENSSVSEKLRKLIIDFSKPNFVQHLSSILPPDAKDIVASILRGLNKPQKQAMKRVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLKKFQVGFLRLGRTQKLHPDVQEFSEEEICKAKSIKSLSALEELYNSQPVVATTCMGVNHPIFTRRRFDFCIVDEASQISQPICLGPLFFAD----RFVLVGDHQQLPPLVKSAEARELGMSESLFKRL-ERNQ-EAVVQLTVQYRMNSKIMALSNKLVYEGRLECASDRVSNAVVQLPHIKTLLLELEFRESQESMWIKDVLEPSNPVCFLNTEKIPALETEEKGGISNWIEAKLVFHLTKLYLKAGCRPSDIGIIAPYRQQLKMISNYFNSLSASAVEVNTVDKYQGRDKSVIIVSFVRSNI------DGKLGDLLKDWRRLNVALTRAKHKLIMLGCVPTLNR 1023
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. UniProt/SwissProt
Match: sp|P51530|DNA2_HUMAN (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Homo sapiens OX=9606 GN=DNA2 PE=1 SV=3) HSP 1 Score: 403.675 bits (1036), Expect = 7.126e-121 Identity = 318/977 (32.55%), Postives = 492/977 (50.36%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELT----NFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQA---MKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIV------QNSKKSIRN-VTFESSSL-SGINFLIKFNLPQK--PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEH--ESINSLEFWKCQCCSS------ELDRSVIFLNY----QPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM---STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQII 3381
W V GDI + S+ W I+ G L++ P L+S TS+ S+ C R+ VL F S + + M+IG+ +H +FQ+ +++E+A + ++ L Y + +EI +E+E Y P KW + K + +MQ+ +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH QV+LY L+ S + + + GLLLY+K G + + N +K+RN++ + I K + Q T LP +I + C+ C + C ++SR + DCS M I++ T HL + E+F W ++ LE S+ + +N +NI + + S I N + E + +L F P + GD +IVS ++ A+ GYV E T + + + + T R+D N + P+ NLS LM+ + + +KLRD I+ + P F +++ L K I+K N QR+ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V ++ T I K ++L L + +V TC+ NH SR FD I+DEA QI+ P+ + PL +F+L GD QLPPLV N AR G+ +SLF +L + + ++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A I + H + LEF+ + E + V FLN P VE + N EAK+ + L F+K G SDIG+I PY QQ ++I ++ S VE+NT+D+YQGRDKSIV++SFV + + +L D RRLNVAI+RAKHKLI++G + L+ Y P++ + + L + ++I
Sbjct: 86 WCSVPVEPGDIIHLEGDCTSDT-WIIDKDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRS-SDPATRQMLIGTVLHEVFQKAIN-NSFAPEKLQELAFQTIQEIRHLKEMYRLNLSQDEIKQEVEDYLPSFCKWAGDFMHKNTSTDFPQMQLSLPSDNSKDNSTCNIEVVKPMDIEESIWSPRFGLKGKIDVTVGVKIHRGYKTKYKIMPLELKTGKESNSIEHRSQVVLYTLL-SQERRADPEAGLLLYLKTG-QMYPVPANHLDKRELLKLRNQMAFSLFHRISKSATRQKTQLASLPQIIEEEKTCKYCSQIGNCALYSRAVEQQMDCSSVPIVMLPKIEEETQHLKQTHLEYFSLWCLMLTLESQSKDNKKNH-QNIWLMPASEMEKSGSCIGNLIRMEHVKIVCDGQYLHNFQCKHGAIPVTNLMAGDRVIVSGEERSLFALSRGYVKEINMTTVTCLLDRNLSVLPESTLFRLDQEEKNCDIDTPLGNLS---KLMENTFVSKKLRDLIIDFREPQFISYLSSVLPHDAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPAIQQFTEQEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKIFDFCIVDEASQISQPICLGPLF----FSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQ--NKSAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLRHFKDVKLELEFYADYSDNPWLMGVFEPNNPVCFLNTDKVPAPEQVEKGGVS-NVTEAKLIVFLTSIFVKAGCSPSDIGIIAPYRQQLKIINDLLARSIGMVEVNTVDKYQGRDKSIVLVSFVRSNK------DGTVGELLKDWRRLNVAITRAKHKLILLGCVPSLN-CYPPLEKLLNHLNSEKLI 1039
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. UniProt/SwissProt
Match: sp|E1BMP7|DNA2_BOVIN (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Bos taurus OX=9913 GN=DNA2 PE=3 SV=3) HSP 1 Score: 400.593 bits (1028), Expect = 7.245e-120 Identity = 316/985 (32.08%), Postives = 489/985 (49.64%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK--KPEISKMQV--------------------IDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFI---LKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQN---DSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESSSLSGINFLIKF-----------NLPQK----PFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDL---NSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-----------MEHESINSLEFWKCQCCSSELDRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFN-ILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQII 3381
W V GDI + SN W I+ G L++ P L+S TS+ S+ C R+ VL F S + + M+IG+ +H +FQ+ + +++E+A + ++ L Y +EI +E+E Y P KW + K + +MQ+ +D+EE+IWS ++G+KGKIDVT+ +T +PLELKTG+ + S EH Q++LY L+ S + + + GLLLY+K G + + +++RN++ + + ++ + T LP +I + C+ C + C ++SR + DS +M IK+ T HL E+F W ++ LE S+ + N ++ S+ ES S G I+ N +K P + GD II+S ++ A+ GYV E T + + T R+D N + P+ NLS LM+ + QKLRD I+ + P F +++ L + K I+K N QR+ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V ++ T I K ++L L + +V TC+ NH SR FD I+DEA QI+ PV + PL +F+L GD QLPPLV N AR G+ +SLF +L + + +++ +L +QYRMN +I+ L N ++Y ++ C +D+VA A I +E + S W + E + V FLN P VE + N EAK+ + L F+K G SDIG+I PY QQ ++I ++ S VE+NT+D+YQGRDKSIV++SFV ++ E+ + +L D RRLNVAI+RAKHKLI++G + LS+ Y P++ + + L + ++I
Sbjct: 87 WCSVPVEPGDIIHLEGDCISN-TWIIDEDFGYLILYPDMLISGTSIASSIRCMRRAVLSETFRS-SDPATRQMLIGTVLHEVFQKAVS-DSFAPEKLQELASQTIQEIRHLKEMYRLKLNQDEIKQEVEEYLPSFSKWAGDFMHKHTSTDFPQMQLSLPSDGSNSNSTCNIEVTNSLDIEESIWSPRFGLKGKIDVTVGVKIHRGCKTKYKIMPLELKTGKESNSIEHRSQLVLYTLL-SQERRADPEAGLLLYLKTG-QMYPVPAKHLDKRELLRLRNQMAFSLFHRINKSTGEKTELAPLPQIIEEQQTCKYCSQMGNCALYSRAVEQQMEDSSVPTSMWPKIKEETQHLKPIHLEYFSLWCLMLTLESQSKDNKRNYQHIWLMPASEME------ESGSCIGSLIRIEHVKTVCDGQYLHNFQRKNGAIPITNLMAGDRIILSGEERTLFALSRGYVKEINSTTVTCSLDRNLSGLPESTLFRLDQEEKNCDIDTPLGNLS---KLMENTRASQKLRDLIIDFREPQFISYLSSVLPHEAKDTVACILKGLNKPQRQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILYACGFSVLLTSYTHSAVDNILLKLAKFKIGFLRLGQIQKVHPDIQKFTEEEICRSKSIKSLALLEELYNSQLIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLF----FSRRFVLVGDHQQLPPLVLNREARALGMSESLFKRLEQ--NKNAVVQLTVQYRMNSKIMSLSNKLTYEGKLECGSDKVANAVINLPNFKDVKLELEFYADYSENPW--LIAAFEPNNPVCFLNTHKVPAPEQVEKGGVS-NIMEAKLVVFLTSVFIKAGCKPSDIGIIAPYRQQLKVISDLLAQSSVGMVEVNTVDRYQGRDKSIVVVSFV-------RSNEDGTLGELLKDWRRLNVAITRAKHKLILLGCVPSLSR-YPPLRKLLNHLNSEKLI 1040
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. TrEMBL
Match: A0A4Y2AMJ2 (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Araneus ventricosus OX=182803 GN=DNA2 PE=4 SV=1) HSP 1 Score: 491.115 bits (1263), Expect = 3.029e-148 Identity = 338/960 (35.21%), Postives = 511/960 (53.23%), Query Frame = 1
Query: 646 HLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISK-----MQVIDVEENIWSTKYGIKGKIDVTLQTN----ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADC----LPSVITNIRLCQSCEYLSLCTMFSRLSQNDS--DCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVL---------EFLSESSNENPIKNIIVQNSKKSIRNVTFESSSLSGINFLIKFNLPQKP-------FYKFVEGDPIIVSSDDG-KKVAIVLGYVVESPGTDILIISSEKPLICSKMTK-----LRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI---MEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIY--FNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMI----RNMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 3387
L G W D++V+VGD ++ ++N I+NH G LV+NP +L+S+T+V +LFC RK L H S N M+IGS +H IFQ+ E+ +I +++ K+ LI ++ EI +++ Y P I WI ++ + K ++ QV +E+NIWS KYGIKGKID+T++T+ +PLELKTGR+T+S EH QV LYILMM ++T+ + GLLLY++D A + + S I++RNEL + K+ D LP + + + C C YL CT++ R D + AM LI T HL E+FVHW NL++L F +E S K + + N S+ + + + ++I F+ + EGD + VS + +VAI +GY+ + + ++S++ L SK+ + RID S + L LM+ KLR+FI+ +PP F + K + + + I+K N Q+RV++ A+ ++DY +IKG PG+GKTS IVA++R+ MG+ V ++SYTHSA+ NIL KL ++F+ + + + LK S+ + F + F + VV TCL+ NHA KFDV I+DEA QI I PL + KFIL GD QLPPLV N AR G+ QSL +LC D+ EL +QYRMN EI++ CN ++YN + C ++ VA A + ++++S+ W Q SE+ +SVIF+N + E N FE + ++++ LK E DIGVI PY +Q ++I R ++ ++E+NT+DQYQGRDK ++IIS V + + I+ND++RLNVAISRAK KLI+IG L+ Y+P K + R+ QII +
Sbjct: 409 RLKGFWTDSLVNVGDSVHVLKEFENNHT-VIDNHSGFLVINPDFLLSSTAVVSTLFCMRKAALNHILPKWSS-GNNVMMIGSLVHDIFQQAVNNHISSSREINDILEKVLKKRENLICLSCQESSEAEIREKVQEYVPSIANWIQKHCTFKGKVPANSLQVTQVEGIEDNIWSPKYGIKGKIDMTVRTSFQQATKILPLELKTGRSTYSAEHIGQVNLYILMMEERTK-EMSDGLLLYLRDVANMKLVTADHNSRRGLIQLRNELAFYTSKWMQRNFDAPDIEHCHLPKPLNSKKFCGKCPYLLPCTVYQRALSEDKNLEADHAMSSLISSTTSHLNSNHLEYFVHWSNLLLLESSSAALTNAFWTEKSTYREKKGMCLSNL--SLDTNVKKIEEIGQVFYIITFHRSEDSDNSSTLSSIGLKEGDYVAVSKERSLTEVAITMGYIAKIYERSV-AVTSDRDL--SKVPQYEDCTFRIDKQVSNSATSCIRNNLMRLMENENEASKLREFIIEKQPPKFSKSLPKDVVLSGRPILKGLNKVQQRVVLKALMAEDYFIIKGMPGTGKTSTIVALVRLLSSMGISVFLTSYTHSAIDNILLKL-KDHMSFVRIGQEARIHQTLKK--FSFETLTRDFTTVDQFKAFMTQQMVVAATCLAVNHAVFKIKKFDVCIVDEASQINQIACIGPLF----HAKKFILVGDDKQLPPLVVNEKARERGMQQSLLERLCS---ADNCMELVIQYRMNKEIMRFCNELTYNGALQCGSEDVATAVLNLDGLKDNMDSIPNWIIQTLQSEIQKSVIFVNTDQVNISHSEKAQDMNDFEVNLVQDILQVLLKCKVSEWDIGVITPYRRQVDLIEDKLRKVNVNDIEVNTVDQYQGRDKDVIIISCVKHNKSTTDCEKKGI--IINDEKRLNVAISRAKRKLIIIGCKSSLAI-YEPFKNLIKCFRSEQIIDL 1347
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. TrEMBL
Match: T1IXE8 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=4 SV=1) HSP 1 Score: 468.003 bits (1203), Expect = 3.255e-140 Identity = 344/965 (35.65%), Postives = 534/965 (55.34%), Query Frame = 1
Query: 601 LTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINR---NLSKKPEISKM-QVIDVEENIWSTKYGIKGKIDVTLQTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILK-FTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLS-QNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLE--------FLS----ESSNENPIKNIIVQNSKKSIRNVTFESSSLSGINFLIKFNLPQKPFYKF----VE-GDPIIVSSDDGKKVAIVLGYVVESPGTDILII----SSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPE--KKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQ--PSFVEADEIYF-NKFEAKITMELVEQFLKIGALESD-IGVIIPYNQQKEMIR------NMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQI 3378
L+V E++ +C L W T V + DI +I+ SN + TI+N G+++VNP +L+S TSV SLFC RK VL F G S +N MVIGS IH IFQE + + I+K + S L Y +G + E++ EI+ + P+IL W + N K + K+ + D+EENIWS +YG+KGKIDV IP+ELKTGR++FS EH QV+LY +M SD+ + + GLL+Y++DG + + +++ I++RNEL ++I + +++ LP + + RLC C Y ++C ++S L +N M+ LI + GHL E ++F+ W +L+ LE F++ ++S+E K + K ++ E+S+ G+ F++KF+ VE G+ +IVS + K+ AI G V + T + + S +P S + R+D +S + L L+D +++ KLR I+ + P F +T S+ + K I+K N Q R ++ ++ ++DY LIKG PG+GKTS IVA++R+ L+G VL++S+THSAV NIL KL I+FL L C K+ + + +K + + E+ ++ TCL +N+ L R +FD IIDEA Q+ + PL + +FIL GD LQLPPLV+N AR NG+DQSLF L + DS +L +QYRMN I+ L N + YN ++ C +D++A +TI +N+L K + S+ L+ SV+F++ P+ +D + N EA+I +++V + LK A D IG+I Y+ Q + IR N+ST VE+NT+DQ+QGRDK ++++SFV + + K E IL D RRLNVAI+RAKHK I+IG++ L+ NY P+ + +++ N I
Sbjct: 347 LSVRSEED-ECVKTCKLKDFWVQTNVKLNDIVNILSTFDSNQVATIDNTNGLIIVNPDHLLSGTSVVSSLFCMRKSVLMEQFRG-SDPANFIMVIGSLIHQIFQEALCKQAFTAQAIGVISKNILMHNSNLHAIYSAGSNETEVMEEIQKFIPQILLWFQQYVPNTKKSGDFIKVTDIYDIEENIWSPRYGVKGKIDV--------IPIELKTGRSSFSPEHEGQVILYSMMCSDRRS-DPEGGLLVYLRDGKTKEVKAEHNQKR-GLIQLRNELIHYIDQPVNENEIGVVHSLPETLNSNRLCSRCPYATVCCVYSSLDDRNILPSDHEMRVLIPEFAGHLSEAHKKYFMKWQHLLRLESSLSRKFSFINGIYCQTSDERQKKGLAFSQMKVERKDSISETSN--GL-FMMKFSKTSDAQSSLDNTDVEIGEMVIVSCEATKEFAIASGTVRDVKKTYVQVEIDKNLSLRPDFSSAV--FRLDRYTSGNVTAIAAGSLIKLIDNNDMSAKLRRLIIDGERPQFIERLTDSVLIG-KKILKKLNSSQTRAVIKSLTAQDYFLIKGMPGTGKTSTIVALVRLLDLLGYSVLLTSHTHSAVDNILIKLKNYGIDFLRLGK----CSKIHPDIHPFAAEKLVQKLTTVKDLKEFYESKNIYATTCLGSNYTCLQRKRFDFCIIDEAAQVVQIACLGPLFYSN----RFILIGDPLQLPPLVQNRMARKNGMDQSLFELL---DSADSTIDLNIQYRMNKTIMSLANELVYNGRLKCGSDEIARSTI----KLNNLT-SKSKLFSTLLEDSVVFIDTDRIPAPELSDNLGIKNAVEAEIILQIV-KLLKKDAFNMDEIGIISIYSAQVKNIRELLLQNNIST--VEVNTVDQFQGRDKEVILVSFVRSTPNMNKLGE-----ILFDFRRLNVAITRAKHKAILIGSISTLT-NYPPVNKLIQIVKENTI 1268
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. TrEMBL
Match: A0A1S3GZW9 (DNA replication ATP-dependent helicase/nuclease DNA2 OS=Lingula unguis OX=7574 GN=LOC106150783 PE=4 SV=1) HSP 1 Score: 463.381 bits (1191), Expect = 5.419e-138 Identity = 324/977 (33.16%), Postives = 508/977 (52.00%), Query Frame = 1
Query: 649 LDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINR-------NLSKKPEISKM---QVIDVEENIWSTKYGIKGKIDVTLQTN-------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFI------LKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESS-----------SLSGINFLIKFN------LPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTK---LRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP------EKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-------MEHESINSLEFWKCQCCSSELDRSVIFLNYQ----PSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMI--RNMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPEN-NSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 3387
L G W DT+V GDI +++ +G+ I++++G+LV+NP L+S TSV G+++C RK +L G N AM+ GS IH +FQ + ++V I K + +Q LI Y T+ + EI+ Y P +L W + + +P S + +V+D+EENIWS +YG+KGKID+T+Q E +PLELKTG+ +FS EH QV LY LM SD+ E + GLLLY+KDG+ + I D + +++RNEL +++ ++ S + LP I N R C C L C ++ R + +D AM LI HL + E+F HW +L+ L + +K+I +++++ + S +L +F+ F+ P+ F +GD ++VS +A+ VV+ T+I +I ++ L + + R+D N S SN + L L+ + +LR+ I+ + P F+ + K K+KHI K N Q+ I+ + KDY+L+KGYPG+GKTS IVA++RI +G+ +L++SYTHSAV NIL KL ++ FL L ++R ILP K + + + +V TCL NH ++ +FD+ IIDEA Q+ P PL KF+L GD QLPP+V+++ AR G+D+SLF +L ++ + EL LQYRMN I++L N + Y + C + VA A + + E + W +C S + V+FL+ +A + N EA+I +L + FL+ G ++G+I PY Q +++ R + EVE+NT+DQYQGRDKS++I+SFV + K + + IL D RRLNVA++RAKHKL+ +G++ L + YQP++ + +L +I I
Sbjct: 390 LSGFWTDTLVQPGDIVNVLGDFDDSGVCHISDNQGLLVINPDVLISGTSVVGTVYCMRKTLLNEKLRGCD-TGNMAMLHGSIIHALFQAVVQQRAFSKDKVEAIVKEILQQKKFLIEMYGQDATEAVVRAEIDKYIPPMLAWTEKYMPPPVHSFGARPSTSDLHVTKVMDIEENIWSPRYGVKGKIDLTVQVKINGKQKCEKVMPLELKTGKPSFSIEHQGQVTLYSLMSSDRRE-DPGGGLLLYLKDGSMKTITADYAKKR-GLLQLRNELVHYLSLNTEKVESESGTTYHLPKLPRPINNPRSCSKCPQLLNCAVYQRSIDHHDLPDDHAMTSLIPATLAHLHQSHLEYFSHWC---LLQELEAKESYKSLKDIWCKSAEQREKEGNCLSDMVLMDRHDAVLALDNGSFIHTFHKQPSKQSPKISLAGFSKGDSLVVSGQTSGHIALCTASVVQVTQTEITVIL-DRNLQNTNLNSDAVYRLDKNDSFNSMASNYTNLSKLLSDNPHSLRLRELIIDARKPEFELTLAKGAIEKVKHIFKSLNKPQKTAILKILMCKDYVLMKGYPGTGKTSTIVALVRILHQLGLSILLTSYTHSAVDNILLKLKKLDLEFLRLG--------RESRIHPEILPYSEGTLTKNIKTVGDLAEFYTAKQIVATTCLGTNHVLFTKRRFDICIIDEASQVLQPTCFGPL----SCAAKFVLVGDPKQLPPVVQSTEARELGMDESLFMRL---DNTGATYELNLQYRMNSSIMRLSNELVYEGALKCGSQDVAEARLQLSNMEDVRAELSGMMPAW-LECALSP--QPVLFLDTDNISATESYDAQGLVKNDLEAEIVCQLAKCFLRGGLSPQELGIIAPYRNQVKVLHTRLGGSGEVEVNTVDQYQGRDKSVIILSFVRSNSKDDSSSKGRGGTEILCDWRRLNVAVTRAKHKLLFVGSVSTL-KKYQPLQKLIQILNPEEICQI 1340
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. TrEMBL
Match: A0A443SN15 (DNA replication ATP-dependent helicase/nuclease DNA2-like isoform X2 OS=Leptotrombidium deliense OX=299467 GN=B4U80_08236 PE=4 SV=1) HSP 1 Score: 440.654 bits (1132), Expect = 4.561e-131 Identity = 318/976 (32.58%), Postives = 505/976 (51.74%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEI-----SKMQVI---DVEENIWSTKYGIKGKIDVTLQTN--------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTM----FSRLSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESSSLSGINFLI-----------KFNLPQKPF----YKFVEGDPIIVSSDDG----KKVAIVLGYVVESPGTDILIISSEKPLICS-KMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPE-KKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQ--PSFVEADE--IYFNKFEAKITMELVEQFLKIGALE-SDIGVIIPYNQQKEMIRNM--STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQI 3378
+ L + L+ N K +K L G W T + VGD +++ + ++ + ++ + G+++VNP L+S+TS++ S+FC RK L F S +NK M IG +H +FQ C + E+ ++ + +Q L Y + + E+ + + VY P I++W+ + + P + K +V+ D+E+NIW T G+KGK+DV LQ +IPLELKTG+ +FS EH QVLLY +MM++ T+ + D GLLLY+K+ + S ++ RNEL + L + + P + N RLC SC+ L C+M F + DSD + +L+ HL + FF W+ L +E ++ +K+ +N+ V E + N +I + N +PF GD + +S D G ++A V+G++ + +I + + EK L S + T LR+DL +S KI L+ L LM + LR++I+ D I K + + I+ N Q++ ++ ++ ++D+ LI G PGSGKT+ IV+++R+ M ++LV+SYTHSA+ NIL K +INFL L + +L+ +L L E F++ NI VV TCLS NH KFD I+DE Q+ L S+ PL +FIL GD QLPP+V+N AR G DQS+FS LC+ N L +QYRMN EI+++ + + Y+ + C + ++A +T+++ + + W + + L +SVIFLN P+ E D + N+ EAKI M +V LK ++ SDIGVI PY +Q E++R M ++EINT+DQYQGR + + E + +L D+RRLNVAI+RA+ KLI++G+ LS Y+P + +L+ +QI
Sbjct: 231 RDLILQLQHN-KITLKCILRGFWSMTNIKVGDSVTVLSNVFADNIVFVDENNGVIIVNPEILISSTSLSSSVFCMRKTWLNEKFVK-SCSTNKEMFIGCIVHELFQNACMMRIKTEQELVKVLDSIIRQSYVLDGCYANKMSTEQFMSHLNVYIPSIVEWMQKYVDADPSLLTSRDEKFRVVKFHDIEDNIWCTDLGLKGKVDVNLQVEIHENKKVIRKSIPLELKTGKASFSFEHEGQVLLYSMMMNEITDSSCDTGLLLYLKEKPQMKFLKVKENVRSSLLQRRNELVQY--------LNDQEVGPPPLNNQRLCYSCDSLFPCSMMFKSFGEMKLTDSDVMRF--ELVPKILDHLSDNHINFFQKWVKLTHMEMSVRDTDRKSLKSFWKENA------VEREQKGFAFANVIIANNVDLEYTLKRINTDCRPFNVKNTCIRVGDRVALSEDKGISMKSRIAFVIGFISKVEEANIYL-TLEKTLNNSFEETTLRVDLLASDKIFSQYLANLLQLMAPEKRESNLRNYIIDGVKAKRDVDINKRILPLCQPIISSLNISQQKAVLKSVQAEDFTLICGTPGSGKTTTIVSIMRVLTAMKKRILVTSYTHSALDNILLKFKKFDINFLRLGFYSRIHPELRKYSLESQLGSLSSVEETKQFYS---NISVVASTCLSLNHPIFELTKFDYCIMDETSQVNLLASLRPLFIT----KRFILVGDSKQLPPVVKNHEARRQGFDQSIFSLLCDEN----TVNLTIQYRMNQEIMRVASELMYDGLLECGSREIATSTLIKRSTCQFEKLWLEEILDNSLTKSVIFLNTDEVPAIEEQDRNNLICNRIEAKIVMLIVNNLLKNFDIKCSDIGVITPYAKQVEILREMCNERTQIEINTVDQYQGR------DKDAVLISTVVSSTEQTTKELLKDKRRLNVAITRARKKLIIVGSQKTLSL-YKPFIHLFSLLKQHQI 1169
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. TrEMBL
Match: A0A4W3IJH4 (Uncharacterized protein OS=Callorhinchus milii OX=7868 GN=dna2 PE=4 SV=1) HSP 1 Score: 434.491 bits (1116), Expect = 2.524e-130 Identity = 321/987 (32.52%), Postives = 486/987 (49.24%), Query Frame = 1
Query: 595 KQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQVI-----DVEENIWSTKYGIKGKIDVTL------QTNEST--IPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFIL-KFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCS---QAMKDLIKDNTGHLCEKDYEFFVHWINLIVLE--------------FLSESSNENP---IKNIIVQNSKKSIRNVTFESSSLSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRS-----------VIFLN-YQPSFVEADEIY--FNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTME-----VEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
K LT+T + L WE T + GDI V+ +G W IN G L+ NP +L+S TSV S+ C R+ VL S + + M+ G+ +H IFQ+ + +++I + + L Y + E+L+EI Y P I+KW + P SK + D+EENIWS ++GIKGKIDVT Q+ T +PLELKTG+ + S EH QV+LY L+ ++ E + + G LLY+K G + N +K+RN+L ++ S A LP V+++ C+ C L C ++SR + + S +K ++ T HL ++F W L ++E LS E I ++ + ++I + ++ + F+ P + GD ++VS + + +A+ GY++ + I + T R D + + +L+ L LM+ S + KLR+ IV +PP F H++ L ++K +I+K N Q++ + + S+DY LI G PG+GKT+ I ++RI G VL++SYT+SAV NIL KL ++ FL L V ++ T I K L L + VV TC+ H SR +FD I+DEA QI P+ + PL D +F+L GD QLPP+V++S AR G+D+SLF +L E NH D++ L +QYRMN +I+ L N + Y ++ C ++QVA AT+ L W +ELD S + FLN + E DE Y N EA++ L LK G +IG+I PY QQ + I + E VE+NT+D+YQGRDKS++I SFV + + N +L D RRLNVA++RAKHKLIM+G + L + Y P++ + D L A Q+IS
Sbjct: 65 KHLTITASQLGDSTEQCVLQDDWESTDIKPGDIVH-VEGENFSGPWVINRDCGYLIWNPDHLISGTSVANSIRCMRRAVL-------SERGTRQMLFGTLVHEIFQKAATSHGFTEDALQKITSKTLRALKYLGEMYSLKLSQAEVLQEISEYVPSIIKWAEDFMHNSPWPSKHSITVTDIEDIEENIWSPRFGIKGKIDVTASVKIHRQSKSQTRIMPLELKTGKESNSIEHRSQVILYTLLSQERRE-DPEAGFLLYLKTGTV-YPVPGNRMDRRELLKLRNQLAFYLANGVVQSSTPKAGLLPPVVSDRLACKFCSQLRNCALYSRAIEEEQAASFVPAELKPVLDQETEHLTRGHLQYFSLWYLLCIMEANTMDSKGGRRLIWMLSAEEREQQGGCIGYLLRNGAAQAISDGQYQHQFIRKNGFI--------PDTALMAGDRVVVSGQEKQLLALSSGYIIGVTESQITCVLDRNLSKLPAETIFRFDHDEGVVGVNYHLANLSKLMEASAVSTKLRELIVDHRPPQFIPHLSTVLPQEVKETVANILKGLNKPQKQAMKRVLLSRDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLASYTNSAVDNILLKLARFKVGFLRLGRVQKVHPNIQKFTDEEICKRKSVHTLAELEELYSSELVVATTCMGVKHPIFSRRRFDFCIVDEASQINQPICLGPLFYAD----RFVLVGDHQQLPPIVQSSEARALGMDESLFKRL-EQNH-DAVVYLNVQYRMNSKIMSLSNKLVYEGKLVCGSEQVAAATL-------QLPRW------NELDSSWLKDALEPRHPICFLNTAKVPAPETDEPYGLGNVTEAQLVYHLTAVLLKAGCRGCNIGIIAPYRQQLKTISALLAKEAAFSAVEVNTVDKYQGRDKSVIIASFVRSNR------DGNLGELLKDWRRLNVAVTRAKHKLIMVGCVPTL-RRYAPLEKLLDNLSAEQMIS 1007
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Cavefish
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:103040169]) HSP 1 Score: 408.683 bits (1049), Expect = 3.709e-121 Identity = 323/1040 (31.06%), Postives = 502/1040 (48.27%), Query Frame = 1
Query: 454 FDDCFNDDFKWDESLIEDKIKQISPNKIQDQDFYYX---XXXXXXXXXXXKQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQV------------------------IDVEENIWSTKYGIKGKIDVTLQTN---------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILK--FTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQN-DSDCS-QAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRN------------VTFESSSLSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPL--ICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIM---EHESINSLEFWKCQC-------CSSELDRSVIFLNYQ--PSF--VEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIK 3345
FDD ++ K +S +E KI + + Y+ + K LT+T + + L WE T V VGDI + SN W I G LV+ P L+S TS+ S+ C R+ VL F G S K M+ G+ +H +FQ+ ++ ++A + L Y T ++ +E+E Y P + +W L K Q+ +D+EENIWS ++G+KGKIDVT + +PLELKTG+ + S EH QV+LY LM S++ ++ + G L+Y+K G I N IK+RN L + + K + + LP+V+++ + C+ C C +++R + +CS + + ++ + HL + ++F HW+ L LE + S + +NI +Q++++ +N VT S + F + ++ GD I VS + + + I GY+ T + S +K L S M R+D + ++L L +LM+ + +KLR IV KPPLF ++ L + K +I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL +I FL L V + T + + L NL +V TC+ H SR +FD I+DEA QI+ PV + PL +F+L GD QLPP+V+N+ AR G+D+SLF +L + +++ +L +QYRMN I+ L N + Y ++ C +++ A A + ++ LE + CQ + E + V FL+ P+ VE + N+ EA + L+ LK G DIGVI PY QQ + I N+ + VE+NT+D+YQGRDKS++I+SFV + PE N +L D RRLNVAI+RAKHKL+M+G+ L + Y P++
Sbjct: 349 FDDGMEEEPKQQKSKLEKKIPENVVLAAGLHNRYWVLDVQETTGSKGYSEKHLTITFSKSREPTETCILKDGWESTPVAVGDIIHLEGESISN-TWVIGRDSGFLVLLPDLLISGTSIASSIRCMRRAVLGEMFKSFDGGS-KQMLNGTIVHDVFQKAAMSMDFSSAKLAKLASEALLSLNYLGQMYSLKLTQADMKQEVEEYLPALSEWAKAYLHTSQHAGKKQLSLKLPSDGQLSRQDSSCSITVTDFVDIEENIWSPRFGLKGKIDVTAGVRIHRKGRKHMDKVVPLELKTGKESNSIEHRSQVILYTLMSSER-RVDAEAGFLVYLKTGNLHPIVG-NHMDRRELIKLRNLLAHHVGKSIVKENGKSQMAPLPAVVSDQQTCKYCPQRRNCAVYNRAVERVPGECSPEDPQAFLQLESEHLSQVHLQYFNHWLLLCSLEAHTMES-KGGRRNIWLQSAQEREKNGGCVGNMVLRDNVTAISDGVYAHYFERRPG--EQTLISLAVGDRIAVSDQNCRFIGIATGYIRGITSTGV-TCSLDKNLSKFPSDMV-FRLDQDEGAGGLSTHLGNLSTLMENTPTSEKLRQLIVEFKPPLFIESLSSVLPREAKDTVANILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILHACGFSVLLTSYTHSAVDNILLKLKKFKIGFLRLGRAQKVHPDILPFTEEHSR-ARGIHTLEQLENLYNKELIVATTCMGVKHPIFSRRRFDFCIVDEASQISQPVCLGPLFYAQ----RFVLVGDHQQLPPIVQNAEARALGMDESLFKRL--EHQKEAVVQLNVQYRMNSVIMSLSNALMYEGKLECGSERTASAVLQLPYRESTVRELEMYVCQPQYTTWVQAALEPQKPVCFLDTSEVPAMETVEKGGVS-NQTEAVLVHSLISLLLKAGCKAGDIGVIAPYRQQLKAISNLLEGAAFTAVEVNTVDKYQGRDKSVIIVSFVRSN------PEGNLGELLKDWRRLNVAITRAKHKLLMLGSAPTL-RLYAPLQ 1364
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Cavefish
Match: upf1 (UPF1 RNA helicase and ATPase [Source:NCBI gene;Acc:103031971]) HSP 1 Score: 122.479 bits (306), Expect = 1.168e-27 Identity = 153/583 (26.24%), Postives = 255/583 (43.74%), Query Frame = 1
Query: 1759 LSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLK-IPISNLSVLGSLMDKSEIGQKLRDFIVHCKPP-------LFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMG-MKVLVSSYTHSAVSNILQKL------------DARE-----INFLYLNGN----HSVCE-----KLKNRTLSYILP-EKKFENLFNF--HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQI---TCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPI 3342
+ G +++ P +K + G I V + G ++AI L S G + + + + K T + S+LK + SV G + K +G ++ D I+ C+ P L D + ++ AVK + + LI+G PG+GKT ++ G VLV + ++ AV + +K+ +RE ++FL L+ S+ E +LK+ T EK++ L L+ N DV+ TC+ A L++++F ++IDE+ Q T P ++P++ + IL GD QL P+V A GL QSLF +L P L++QYRM+ + + I Y + A D++ +F W + D+ + F Q + Y N+ EA ++ + LK GA IG+I PY Q+ +++ M EVEI ++D +QGR+K +I+S V +A E+ LND RRLNVA++RA++ +I++GN LS+ QP+
Sbjct: 337 MQGDEICLRYKGDMAPLWKGI-GHVIKVPDNYGDEIAIEL---RSSAGAPVEVPHNFQVDFVWKSTSFD-RMQSALKTFAVDETSVSGYIYHKL-LGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVK------------------TVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSSDEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVL---GAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRP---IRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRIK----------KGFDFQW------PQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCV-------RANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSK--QPL 864
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Cavefish
Match: ighmbp2 (immunoglobulin mu DNA binding protein 2 [Source:NCBI gene;Acc:103034943]) HSP 1 Score: 108.997 bits (271), Expect = 1.417e-23 Identity = 107/440 (24.32%), Postives = 183/440 (41.59%), Query Frame = 1
Query: 2158 QRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLF-------------------------------NFHNLIENIDVVGV--------TCLSANHAA--------LSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICE-LKLQYRMNIEILKLCNTISYNNQITCATD-------QVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM---STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMI 3303
Q+ + A+ KD +I G PG+GKT+ +V +I V KVL + ++ AV N++++L + L L + E ++ +L ++ + N+ + +++ + V + L+ N A L FD+V+IDE Q + LLK K IL GD QLPP +++ TA GL SL +L + + DS+ L QYRMNI I++ + Y ++T +A +E S+ L C +EL+ + ++ N+ E I V+ + G DI VI PYN Q +++R E+EI ++D +QGR + L ++ L + RR+NVA++RA+ +L+++
Sbjct: 194 QKEAVSFALSQKDVAIIHGPPGTGKTTTVVEVILQAVKQDQKVLCCAPSNVAVDNLVERLAKSKAKVLRLGHPARLLESVQKHSLDAVVAQSDSSNIIADVRKDMDKAFSQIKKVRDKGQRSSLRREIGDLRKELKSREEVAIKQILKKASVVLATNTGASDDGPLKYLPSEHFDLVVIDECAQALESSCWIALLKAR----KCILAGDYKQLPPTIKSQTAAAKGLSVSLMERLIQ-KYGDSVVRMLTTQYRMNIAIMQWASEQMYQGRLTAHPSVEKHLLKDLAGVAHVEETSMPLLLIDTAGCGLNELEDT------------DEQSKGNQGEVDIVSVHVKALTEAGVHAKDIAVIAPYNLQVDLLRQKLSNEHPELEIKSVDGFQGR-------EKEAVLLSLVRSNRKGEVGFLAEDRRINVAVTRARRQLMVV 609
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Cavefish
Match: mov10a (putative helicase mov-10-B.1 [Source:NCBI gene;Acc:103034927]) HSP 1 Score: 102.064 bits (253), Expect = 2.284e-21 Identity = 128/459 (27.89%), Postives = 208/459 (45.32%), Query Frame = 1
Query: 2149 NPDQRRV---IMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMG--MKVLVSSYTHSAVSNILQKLDAREINFLYLNGNH--SVCEKLKN-RTL---------------SYILPEKKFENLFNFHNLIENIDVVGVTCLSANH---AALSRLKFDVVIIDEAGQITLP---VSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN------HPDS-------ICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNY---------QPSFVEADEI-----YFNKFEAKITMELVEQFLKIGALE-SDIGVIIPYNQQKEMIRN-MSTM-------EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQN 3330
NP+Q I+S LI G PG+GKT +V I+ G VL + ++SA + ++L Y++G+H +C ++ RT+ S+ILP K E L + V+ VT ++A + F V IDEAGQ P V I LL + KG+ +L GD QL P++R+ A+ +GL SL +L N H S + +L YR + IL++ N + Y+N++ DQ ME E+ E Q VIF PSF EI Y K ++L + + L+ +DIG+I PY +Q E IR + T+ ++++ +++++QG++K ++++S V S K ++ + L +++R NVA++RAK LI++GN L++N
Sbjct: 516 NPEQSTAVQHILSGHSKPGPYLIFGPPGTGKTVTLVEAIKQVNRSGTVAHVLACAPSNSACDLLCERL------LRYVDGHHLYRLCASSRDPRTMPQKLLKHSNWDEGQDSFILPSK--ETLMGY-------SVIVVTLITAGRLVSGGVPVGHFTHVFIDEAGQAVEPECVVGIAGLLHPE--KGQVVLAGDPQQLGPILRSPLAQQHGLGLSLLERLMTQNSLYQKTHDGSQAYNSCFVTKLLRNYRSHPAILEIPNELFYDNELQVFADQ------MEREAFCHWEHLPKQGF------PVIFHGVMGKDEREANSPSFFNVSEIEVLISYLQK------LKLTQGKKGLPKLKPADIGIIAPYRKQVEKIRKALQTVKELKQWNDIKVGSVEEFQGQEKRVIMVSAVRSSISYVKMDQDFNIGFLTNEKRFNVAMTRAKALLIVVGNPVILNKN 939
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Cavefish
Match: ENSAMXT00000014945.2 (Mov10 like RISC complex RNA helicase 1 [Source:NCBI gene;Acc:103044404]) HSP 1 Score: 100.523 bits (249), Expect = 6.456e-21 Identity = 115/422 (27.25%), Postives = 194/422 (45.97%), Query Frame = 1
Query: 2149 NPDQR----RVIMSAIFSKDYLLIKGYPGSGKTSCIV-AMIRIF-VLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENID------VVGVTCLSAN---HAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKL-------CEFN--HPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVE--ADEIYFNKFEAKITMELVEQFLK-----IGALESDIGVIIPYNQQKEMIR----NMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGN 3309
NP QR R++ Y+L G PG+GKT +V A+++++ + G ++LV + ++SA I +L E FL L + L E E L + E+I +V TC SA L F V +DEAGQ T P + +PL + + G+ +L GD QL P+V++ A GL SL +L C N +P + +L YR + +L L + + Y ++ + + ++ + + F +IF + S + ++ +FN EA M Q K I A +DIG+I PY +Q E IR + ++++ +++++QG++ ++I+S V ++ L + N L++ +R NVAI+R+K LI IGN
Sbjct: 741 NPAQRQAVKRILGGECRPTPYVLF-GPPGTGKTITLVEAILQVYHRVTGSRILVCTPSNSAADLICIRL--HESGFL-------PAASLARVNATCRLEESIPEVLHQYSRAGEDIRNACFHRIVVSTCSSAGMFYQIGLRIGHFTHVFLDEAGQATEPEAQIPLGLLSERTGQIVLAGDPKQLGPVVKSKLAEAFGLGVSLLERLMANPLYSCSENGYNPLLVTKLVYNYRSHEVLLALPSRLFYGGELCVRSQRSVVDSLCHWGRLPTKGF------------PLIFHGVRGSEMREGSNPSWFNPVEAVQVMLYCCQLAKRLYNPIPA--TDIGIISPYRKQVEKIRVLLHKVGLGDIKVGSVEEFQGQEFLVIILSTVRSNEDLSGSDLQNVLGFLSNPKRFNVAITRSKALLITIGN 1138
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Sea Lamprey
Match: DNA2 (DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939]) HSP 1 Score: 281.567 bits (719), Expect = 2.293e-80 Identity = 233/840 (27.74%), Postives = 351/840 (41.79%), Query Frame = 1
Query: 601 LTVTLEDNYKCQVK-----LHLDGMWEDTVVHVGD--ICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPE---------------------------ISKMQVIDVEENIWSTKYGIKGKIDVTL--------QTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFT----------------------SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMK----------DLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESSSLSGINFLIKFNL----------PQ-----------------KPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPI-SNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIK----HIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLN-------GNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKL-CEFNHPDSICELKLQYRMN 2778
LT L+D++ LHLDG E + G+ C+ V + + + N G +VVNP L+S T+VT + C R+ VL F G + M++G+ +H +FQ +R A Q L Y+ G + E+L E+E YF I +W +R L PE +S + DVEE++WS ++G+KGK+D + + +PLELKTG+ + S EH QV+LY +++ + + G LLY+K G + + IK+RN+L + + Q LP I I C++C L C ++ R + ++ S + L+ + T HL +F W L +LE +E ++ ++ + + +L G PQ P GD +IVS D +AI V+ ++ +D++ S + ++L L L+ + KLR+ ++ K P F ++ L V K I+K N QR+ + + S+DY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL + FL L G H+ E+ R PE+ L E VV TC+ HA SR FD I+DEA QI PV + LL + +F+L GD QLPPLV NS AR G+D+SLF +L C H +++ L LQYRMN
Sbjct: 48 LTCVLKDDWALAPVSVGDVLHLDGAVE-VPLRGGEEATCASV----TAAVLEVTNDAGFVVVNPDLLLSGTTVTNGIRCPRRAVLSERFKGGGEWGTRQMLLGTLLHEVFQRAALQRDFSDAALRSAASATLAQLQHLAEMYKLGVSQAELLGEMEEYFSAIGRWASRYLEGGPEPNSASHGLRMPIKITRRAGEGDASACTVSVCHLGDVEESVWSPRFGLKGKVDAMATVRIHRRARCDGKKVPLELKTGKESQSIEHKAQVVLYTTLLTQERWGDPGLGFLLYLKSGGMHPVPASRL-DMRELIKLRNQLAHSLANGVWWPPRTAASGAGAGGRRKEEEEDRQEPRPAPLPEPIAEIFTCRNCSQLGNCAVYHRAVEEEARGSSDVPASSSWPVEFVSLLAERTQHLSPAHLRYFSRWYLLCLLEAQTEQCKGGRHNLWVLPPHERQAKGLCVCDLTLLGPAVATPDGRFFIQRFRRRGPQGGWGAAGLTPDLEGQGSAPQGCVAVGDRVIVSGKDPATIAIATATVLSVTNEEVQCQVDRDLGRFGAAFLFSLDVDESFSGSVDTHLGNLSRLLSNTPASAKLRELVIDLKRPEFLERLSDVLPVDAKDTVAQILKGLNRPQRQAMKRVLLSRDYTLIVGMPGTGKTTTICTLVRILHACGHSVLLTSYTHSAVDNILLKLVPHRVPFLRLGRPAKVHAGVHAFTEEELCRQHDVRTPEQ-------LQQLYEGQRVVATTCMGLRHAIFSRRVFDFCIVDEASQIGQPVCLGLLL----HASRFVLVGDHHQLPPLVLNSRARAMGMDESLFKRLEC---HAEAVVYLNLQYRMN 867
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Sea Lamprey
Match: ighmbp2 (immunoglobulin mu DNA binding protein 2 [Source:ZFIN;Acc:ZDB-GENE-050419-258]) HSP 1 Score: 122.094 bits (305), Expect = 1.209e-28 Identity = 107/448 (23.88%), Postives = 182/448 (40.62%), Query Frame = 1
Query: 2158 QRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILP---------------EKKFENLFN----------------------------FHNLIENIDVVGVTCLSANHAALSRL----KFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITC----------------ATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMST---MEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMI 3303
QR ++ A+ ++ +I G PG+GKT+ +V +I+ V G KVL + ++ AV N++++L + L L + + +++R L +L +K L N N++++ DV+ T A +L F V ++DE GQ S +PLL+ + +L GD QLPP + + A GL SL +L E ++ L +QYRMN I+ + Y ++ ATD+ A A ++ ++ C +EL ++ N E I V+ + G DI VI PYN Q +M+R + E+EI ++D +QGR + L ++ L + RR+NVA++RA+ L ++
Sbjct: 199 QREAVIFALAQREVAIIHGPPGTGKTTTVVEIIQQTVRRGEKVLACAPSNVAVDNLVERLACAGVRVLRLGHPARLLDSVRSRCLDAVLARGDDAGIVRDVRSDMDKVHAKLRNGSERGERARLQAEMRSLRKELREREDTAMRNILQSADVILATTTGAWAGGPLKLLPPAHFAVAVVDECGQAVEAASWLPLLQAP----RCVLAGDHRQLPPTIVSQRAAEGGLAVSLMERLVERLGSSAVRMLTVQYRMNAAIMDWASQHVYQGKLEAHPSVASHLLKDLPGVEATDETALALLL-------VDTAGCGLLETEL--------------AGEQSKGNAGEVDIVSAHVQALIDAGVKAEDIAVIAPYNLQVDMLRQKLSDRHPELEIRSVDGFQGR-------EKEAVILSLVRSNRKGEVGFLAEDRRINVAVTRARRHLAVV 614
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Sea Lamprey
Match: upf1 (UPF1 RNA helicase and ATPase [Source:ZFIN;Acc:ZDB-GENE-040426-2836]) HSP 1 Score: 113.62 bits (283), Expect = 1.367e-25 Identity = 137/509 (26.92%), Postives = 227/509 (44.60%), Query Frame = 1
Query: 1972 LNSSLK-IPISNLSVLGSLMDKSEIGQKLRDFIVHC-------KPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLM-GMKVLVSSYTHSAVSNILQKL------------DARE-----INFLYLNGN----HSVCE-----KLKNRTLSYI-LPEKKFENLFN--FHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPI 3342
+ S+LK + SV G + K +G ++ D ++ C P L D + ++ AVK + + LI+G PG+GKT ++ VLV + ++ AV + +K+ +RE ++FL L+ S+ E +LK+ T EK++ L L+ N DV+ TC+ A L+++++ ++IDE+ Q T P ++P + + IL GD QL P+V A GL QSLF +L P L++QYRM+ + + I Y + + V A ++ +F W ++ D+ + F Q + Y N+ EA ++ + LK GA IG+I PY Q+ +++ M EVEI ++D +QGR+K +I+S V +A E+ LND RRLNVA++RAK+ +I++GN LS+ QP+
Sbjct: 411 MQSALKTFAVDETSVSGYIYHKL-LGHEVEDVLIKCMLPKRFTAPGLPDLNHSQVYAVKT------------------VLQRPLSLIQGPPGTGKTVTSATIVYHLAKQNSGPVLVCAPSNIAVDQLTEKIHRTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSLPELQKLQQLKDETGELSSADEKRYRALKRNAERELLMNADVICCTCVGAGDPRLAKMQYRSILIDESTQATEPECMVPTVL---GAKQLILVGDHCQLGPVVMCKKAAKAGLCQSLFERLVVLGIRP---IRLQVQYRMHPALSAFPSNIFYEGSLQ---NGVTAAERVK----KGFDFTW------TQPDKPMFFYVTQGQEEIASSGTSYLNRTEASNVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHAKLYQEVEIASVDAFQGREKDFIILSCV-------RANEHQGIGFLNDPRRLNVALTRAKYGVIIVGNPKALSK--QPL 872
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001658.1 (pep scaffold:Pmarinus_7.0:GL482735:13451:15897:-1 gene:ENSPMAG00000001498.1 transcript:ENSPMAT00000001658.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 72.7886 bits (177), Expect = 1.697e-14 Identity = 67/226 (29.65%), Postives = 108/226 (47.79%), Query Frame = 1
Query: 2782 EILKLCNTISYNNQITCATDQVAFATIMEHESINSLEF-----------WKCQCCSSELDRSVIFLNYQPSFVEADEIY-----FNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTME---------VEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIIS 3384
EI+ L N ++Y + C +++V+ + S+ L W Q + + DR V+FLN + V A+E+ N EA++ + +K G D+G+I PY QQ + +EINT+D+YQGRDK ++I+ FV G E +L D+RRLNVA++RAKHKL+++ + Y P+ + D + +IS
Sbjct: 2 EIMSLSNVLTYGGLLACGSERVSTCRL----SLPGLPAFLDGMGGGNHPWLTQ--ALDPDRPVLFLNTE--KVPAEEMVDQGGVKNPTEAQLIAIIAASLIKAGCRTKDVGIISPYRQQLGAVSAALAALPCPAAALAGLEINTVDKYQGRDKPVIIVGFVRTCATAGILGE-----LLKDRRRLNVAVTRAKHKLLLL-GCLPTLKLYTPLAALIDHMATQGMIS 213
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008661.1 (pep scaffold:Pmarinus_7.0:GL478401:184160:191851:-1 gene:ENSPMAG00000007841.1 transcript:ENSPMAT00000008661.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 68.1662 bits (165), Expect = 5.982e-12 Identity = 87/316 (27.53%), Postives = 139/316 (43.99%), Query Frame = 1
Query: 2431 KKFENLFNFHNLIE--NIDVVGVTCLSANHAALSR-LKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADE-----IYFNKFEAKITMELVEQFLKIGAL-ESDIGVIIPYNQQKEMIRNMSTME----VEINTIDQYQGRXXXXXXXXXXXX------XXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYL 3321
KK++ L +IE DVV TC +A L + L V ++DE T P ++PL ++ + +L GD QL P V N TA G+++S+F + + L QYRM+ +I + Y+ T T + A SI L + C + V + + V + E NK E + + LV + +K G++ E DI ++ PYN Q I+ E V TI + QG + V++S V + + + + D+ ++NVA++RAK L +IGN D L
Sbjct: 101 KKYKKLIKDAKVIELKKSDVVITTCSTAATVILLKNLDPRVCLVDECAMCTEPECLIPLTSY-KDIAQVVLLGDHKQLRPFVLNRTAAKLGMEKSMFER-----YAKRAIMLDTQYRMHPDICEFPAKQFYSE--TNKTLKTAKEVFGRPNSI--LGYAHKPSCPTLFGHVV---GKEVTLVVSTEEGSENSKANKAEVEQVVRLVSELIKKGSIAEKDIAILTPYNAQANEIQKALEKEHIKKVTATTITKSQGSEWRYVLLSTVRSCSLDEFKANPSRGWQISRLGFIIDENQVNVALTRAKEGLCIIGNQDLL 403
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Yeast
Match: DNA2 (Tripartite DNA replication factor; single-stranded DNA-dependent ATPase, ATP-dependent nuclease, helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; component of telomeric chromatin with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress; human homolog DNA2 complements yeast dna2 mutant [Source:SGD;Acc:S000001207]) HSP 1 Score: 228.409 bits (581), Expect = 2.735e-61 Identity = 163/438 (37.21%), Postives = 243/438 (55.48%), Query Frame = 1
Query: 2053 LRDFIVHCKPPLF-DCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHA--ALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSS--------ELDRSVIFLNYQ--PSFVEADEI--YFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTMEV----EINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGN 3309
LR +V + P F D + + K+ + N +Q+ I + ++DY LI G PG+GKT+ I +I+I V G +VL++SYTHSAV NIL KL I+ + L H K+ T Y+ ++ ++ + I + VV TCL N L+ FD VI+DEA QI++PV++ PL + +FI+ GD QLPPLV+N AR+ GL++SLF CE HP+S+ EL LQYRM +I+ L N + Y+N++ C ++V FA +E +L ++ + +S E R V+FLNY P +E E N EA++T++ VE L G DIGV+ Y Q +++ + V EI T DQ+QGRDK +IIS V ++ +L N +L + RR+NVA++RAK KLI+IG+
Sbjct: 1021 LRSLLVDNRAPKFRDANDDPVIPYKLSKDTTL-NLNQKEAIDKVMRAEDYALILGMPGTGKTTVIAEIIKILVSEGKRVLLTSYTHSAVDNILIKLRNTNISIMRLGMKH----KVHPDTQKYVPNYASVKSYNDYLSKINSTSVVATTCLGINDILFTLNEKDFDYVILDEASQISMPVALGPL----RYGNRFIMVGDHYQLPPLVKNDAARLGGLEESLFKTFCE-KHPESVAELTLQYRMCGDIVTLSNFLIYDNKLKCGNNEV-FAQSLELPMPEALSRYRNESANSKQWLEDILEPTRKVVFLNYDNCPDIIEQSEKDNITNHGEAELTLQCVEGMLLSGVPCEDIGVMTLYRAQLRLLKKIFNKNVYDGLEILTADQFQGRDKKCIIISMVRRNSQL------NGGALLKELRRVNVAMTRAKSKLIIIGS 1441 HSP 2 Score: 95.5153 bits (236), Expect = 3.368e-20 Identity = 102/346 (29.48%), Postives = 170/346 (49.13%), Query Frame = 1
Query: 754 ILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVR-EIAKRLSKQPSQLITFY--------ESGWTDEEILREIEVYFPRILKWINRNLSKK--------------PEISKMQVIDVEENIWSTKYGIKGKIDVTLQTN-----ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKD-GAERFIFNDNSPSVISTIK----IRNELT-NFI--LKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMKDLIKDN----TGHLCE--KDY-EFFVHWINLIVLE 1662
+LV+NP L S TSV S+ C R+ +LQ F G+ + M +G+ +H + Q+ + + L N++ EI + K S L T+ E + E +++E + IL ++N+ +SK IS VID+EENIWS YG+KG +D T++ N + +PLE+KTG++ S + Q L+Y L+++D+ E+ I+ LL + +D +F PSV+ +IK RN ++ NF L+ Q + LP ++ + C SC C + ++L + D + L++ T HL + +Y EFF + +LI E
Sbjct: 499 LLVLNPDVLFSATSVGSSVGCLRRSILQMQFQDPRGEPSLVMTLGNIVHELLQDSIKYK-LSHNKISMEII--IQKLDSLLETYSFSIIICNEEIQYVKELVMKE---HAENILYFVNKFVSKSNYGCYTSISGTRRTQPISISNVIDIEENIWSPIYGLKGFLDATVEANVENNKKHIVPLEVKTGKSR-SVSYEVQGLIYTLLLNDRYEIPIEFFLLYFTRDKNMTKF------PSVLHSIKHILMSRNRMSMNFKHQLQEVFGQAQSRFELPPLLRDSS-CDSCFIKESCMVLNKLLE---DGTPEESGLVEGEFEILTNHLSQNLANYKEFFTKYNDLITKE 827
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Yeast
Match: NAM7 (ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress [Source:SGD;Acc:S000004685]) HSP 1 Score: 107.071 bits (266), Expect = 6.746e-24 Identity = 110/426 (25.82%), Postives = 186/426 (43.66%), Query Frame = 1
Query: 2173 MSAIFSKDYLLIKGYPGSGKTSCIVAMI-RIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNG--NHSVCEKLKNRTLSYIL---PEKKFENLFNFHN-----------------------LIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYF-NKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTM----------EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQN 3330
+S + + LI+G PG+GKT ++ + + ++LV + ++ AV ++ KL + + L V + N L ++ + + +NL + ++ DVV TC+ A L KF V+IDE+ Q + P ++P++K + IL GD QL P++ A GL QSLF +L H L++QYRMN + + + + Y + TI + NS W + + + NY + A+ F N+ EA ++ + + G IGVI PY Q+ I M +VE+ ++D +QGR+K +I+S V +A E + L D RRLNV ++RAK+ L+++GN L++N
Sbjct: 417 VSHVLQRPLSLIQGPPGTGKTVTSATIVYHLSKIHKDRILVCAPSNVAVDHLAAKLRDLGLKVVRLTAKSREDVESSVSNLALHNLVGRGAKGELKNLLKLKDEVGELSASDTKRFVKLVRKTEAEILNKADVVCCTCVGAGDKRLD-TKFRTVLIDESTQASEPECLIPIVK---GAKQVILVGDHQQLGPVILERKAADAGLKQSLFERLISLGH--VPIRLEVQYRMNPYLSEFPSNMFYEGSLQNGV------TIEQRTVPNSKFPWPIRGIPM-----MFWANYGREEISANGTSFLNRIEAMNCERIITKLFRDGVKPEQIGVITPYEGQRAYILQYMQMNGSLDKDLYIKVEVASVDAFQGREKDYIILSCV-------RANEQQAIGFLRDPRRLNVGLTRAKYGLVILGNPRSLARN 818
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Yeast
Match: HCS1 (Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities [Source:SGD;Acc:S000001500]) HSP 1 Score: 105.145 bits (261), Expect = 1.720e-23 Identity = 123/506 (24.31%), Postives = 220/506 (43.48%), Query Frame = 1
Query: 2035 SEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNP---DQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMG--MKVLVSSYTHSAVSNILQKLDAREINFLYL-----------NGNHSV-----------------------CEKLKN-----------RTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLK--------FDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTAR--INGLDQSLFSKLCE-FNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVE----ADEIY-----FNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQ----KEMIRN-MSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQ 3327
S I K+ ++V+ +P + + + ++I NP D ++ ++ + D +I G PG+GKT ++ +I+ ++ ++L+ ++ +V IL++L N L L N HS+ +KLKN + L L +++F+ + +LI +V T ++ L L FD +IIDE Q P +PL+ K +L GD QLPP ++ + I+ L+ +LF ++ + F D + L +QYRMN +I++ + YN ++ +A AT+ I+ L + D + + Y E ADE +N+ E I E +E E+ IGVI PYN Q K++I + + ++EI+T+D +QGR L ++ E L ++RRLNVA++R + +L+++GN++ L +
Sbjct: 174 SPIQDKIIQYLVNERPFIPNTN-------SFQNIKSFLNPNLNDSQKTAINFAINNDLTIIHGPPGTGKTFTLIELIQQLLIKNPEERILICGPSNISVDTILERLTPLVPNNLLLRIGHPARLLDSNKRHSLDILSKKNTIVKDISQEIDKLIQENKKLKNYKQRKENWNEIKLLRKDLKKREFKTI---KDLIIQSRIVVTTLHGSSSRELCSLYRDDPNFQLFDTLIIDEVSQAMEPQCWIPLIAHQNQFHKLVLAGDNKQLPPTIKTEDDKNVIHNLETTLFDRIIKIFPKRDMVKFLNVQYRMNQKIMEFPSHSMYNGKL------LADATVANRLLID-LPTVDATPSEDDDDTKIPLIWYDTQGDEFQETADEATILGSKYNEGEIAIVKEHIENLRSFNVPENSIGVISPYNAQVSHLKKLIHDELKLTDIEISTVDGFQGR-------EKDVIILSLVRSNEKFEVGFLKEERRLNVAMTRPRRQLVVVGNIEVLQR 655
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Yeast
Match: SEN1 (ATP-dependent 5' to 3' RNA/DNA and DNA helicase; subunit of the exosome-associated Nrd1p complex that mediates 3' end formation of snRNAs, snoRNAs, CUTs and some mRNAs; helicase-independent role in transcription-coupled repair; coordinates replication with transcription, associating with moving forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of juvenile ALS [Source:SGD;Acc:S000004422]) HSP 1 Score: 75.8702 bits (185), Expect = 3.437e-14 Identity = 91/311 (29.26%), Postives = 145/311 (46.62%), Query Frame = 1
Query: 2479 DVVGVTCLSANHAALSRL--KFDVVIIDEAGQITLPVSIMPLLKMDQNKGKF-ILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQIT--CATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESD----IGVIIPYNQQ-----KEMIRNMSTM---EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDV 3360
D++ T + H L+ + KFD VIIDEA Q T SI+PL + GK I+ GD QLPP V + A +QSLF ++ + + P L +QYRM+ I K ++ Y ++ D + + E + +F+ E + + Y N E ++ +ELV+ + + D IG+I PY +Q KE R M ++ NTID +QG++K I++IS V D +S L D RR+NVA++RAK + ++G+ L+++ +I D
Sbjct: 1563 DIICSTLSGSAHDVLATMGIKFDTVIIDEACQCTELSSIIPL----RYGGKRCIMVGDPNQLPPTVLSGAASNFKYNQSLFVRMEKNSSP---YLLDVQYRMHPSISKFPSSEFYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFDIISGRQEQNAKTM-------------SYTNMEEIRVAIELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGFQGQEKEIILISCVRADDT------KSSVGFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKLWRDLIEDA 1847
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Nematostella
Match: EDO44587 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV67]) HSP 1 Score: 115.546 bits (288), Expect = 4.813e-28 Identity = 79/275 (28.73%), Postives = 131/275 (47.64%), Query Frame = 1
Query: 661 WEDTVVHVGDICSIVDPIKSNGLWTI--NNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLS--KKPEISKM--------------------QVIDVEENIWSTKYGIKGKIDVTLQT-------------------NESTIPLELKTGRTTFSR----EHTCQVLLYILMMSDKTEMNIDKGLLLYIKDG 1344
WE T V GDI + N I N ++V+P L+S T++ G+ C R+ +L F + +N+AM++G+ +H +F + + + + L +Q + L FY G T+ + L +++ P +W R + P++S M Q+ D+EEN+WS ++G+KGK+D T++ +++PLELKTG+ FS+ EH QV+LY L++SD+ ID GLL Y+K G
Sbjct: 36 WEGTDVEPGDIVHVTGVFDINSGVCILDNTANNYIIVHPDTLISGTTIAGATRCLRRSILNERFR--TEDANEAMLMGTLLHDLFDRAMKSNDFSHDALLCAVQGLLEQFTYLDGFYGLGMTECDALNKVKELLPAFSEWAWRYVCDLPDPDLSTMDYKDGSCDTGGTEMPSVCVSQLRDIEENVWSPRFGLKGKVDATVEVKIDRRPMSRRHRPGMDNHLTTASVPLELKTGK-LFSKLGSVEHRAQVILYTLLLSDRYRHGIDTGLLYYMKSG 307
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Nematostella
Match: EDO34496 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SP82]) HSP 1 Score: 94.3597 bits (233), Expect = 8.332e-20 Identity = 123/473 (26.00%), Postives = 211/473 (44.61%), Query Frame = 1
Query: 2068 VHCKPPLFDCHITKSL-AVKIKHIMKICNPDQRRVIMSAIFSKDY---LLIKGYPGSGKTSCIVAMIR--IFVLMGMKVLVSSYTHSAVSNILQKLDAR----EINFLYLNGNHSVCEKLKN--RTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANH---AALSRLKFDVVIIDEAGQITLPVSIMPLLKM----DQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSK------------------LCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLK---IGALESDIGVIIPYNQQKEMIRNM----STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIH 3354
+H PPL D K L VK++ N +QR+ ++S + LI G PG+GKT +V I+ + V KVL + ++SA IL+++ ++ L LN L + + +S I F F I N +V T ++A A + F V IDE+GQ P ++PL + + G+ +L GD QL P++R+ A GL SL + L E+ P + +L YR + IL+L N + Y++++ D++ ++ + + F + + PSF FN EA ++ ++ + L ++IGVI PY QQ IR + + +++ +++++QG ++ ++IIS V S + K LN+++R NVAI+RA+ LI++GN L ++ + IH
Sbjct: 42 IHHLPPLCDDDKVKRLFDVKLED-----NQEQRQAVVSIVKGTSRPHPYLIFGPPGTGKTITVVESIKQVLHVFPNSKVLACAPSNSAADLILERVMKHNVIPKMKMLRLNAFGRSYASLSDSIKVVSCIHDGDFF---FPGKEEIMNKRLVVCTLVTAGRLVSADIPDTHFTHVFIDESGQALEPECMVPLAGLLNPENPGGGQLVLAGDPQQLGPVLRSPLAIKYGLRMSLLERLMTRVAAYGRITEDEEDELGEYE-PAMLTKLLKNYRSHPAILELPNGMFYDDELETCADKLKRESLCNWSKLPTKGF---PILFDGVLGKDLREGKSPSF-------FNPEEAATVVKHIKDLRDSRGVRVLMAEIGVISPYRQQVSKIRQLLHKNNITGIKVGSVEEFQGDERRVIIISTVRSSQEFLKMDAQFKLGFLNNRKRFNVAITRAQALLIVVGNPHVLCKDSHWGRFIH 495
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Nematostella
Match: EDO45106 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTX4]) HSP 1 Score: 87.0409 bits (214), Expect = 1.000e-17 Identity = 106/433 (24.48%), Postives = 199/433 (45.96%), Query Frame = 1
Query: 2155 DQRRVIMSAIFSKD-----YLLIKGYPGSGKTSCIV-AMIRIF-VLMGMKVLVSSYTHSAVSNILQKLDA----REINFLYLNGNHSVCEKLKNRTLSYILPEK--KFENLFNFHNLIENIDVVGVTCLSANHA---ALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCE-----------FNH----PDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEA--DEIYFNKFEAKITMELVEQFLKIGAL----ESDIGVIIPYNQQKEMIR----NMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQN 3330
+++R ++ I S YLL G PG+GKT +V A++++F + +V+ + ++SA + +L +E + + LN V E +PE K+ + L + ++ TC +A L F V +DEAGQ T P ++P+ G+ IL GD QL P++R+ A GL+ SL +L +H P + +L YR + +L+L + + Y++++ ++ E ++ E Q ++F + + +FN EA + V Q LK A +D+G+I PY +Q E IR + EV++ +++++QG+++ +IIS V ++ + ++ L++ +R NVA++RA+ L+++GN L ++
Sbjct: 18 ERQRAAVARILSGQSRPTPYLLF-GPPGTGKTVTLVEAILQVFHRVPSSRVIACAPSNSAADLMAVRLHRSGFIQEGDMIRLNAFQRVQE----------IPESINKYCVDTDSLQLAAHYRIIVCTCSTAGQLFSLGLKPGHFTHVFVDEAGQATEPECLIPIGLAAGEDGQIILAGDPFQLGPVLRSPVAISYGLNVSLLERLMSGLLYARDETRFVDHGCYDPLLVTKLVNNYRSHPSLLRLPSALFYHSELRVCAERTM------REQLSQWEMLPTQ------GTPLVFHGLKGEDMREGNSPSWFNPVEAVQVVRYV-QALKNSATCPIKLADLGIITPYKKQVEKIRLLLSRVGIDEVKVGSVEEFQGQERPAIIISTVRSTEAMVGFDVAHTIGFLDNPKRFNVAVTRAQSLLVVVGNPHVLCRD 426
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Nematostella
Match: EDO45137 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTS2]) HSP 1 Score: 68.1662 bits (165), Expect = 2.558e-11 Identity = 78/313 (24.92%), Postives = 144/313 (46.01%), Query Frame = 1
Query: 2476 IDVVGVTCLSA------NHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDS--ICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYF-----NKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNM------STMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHD 3357
+D++ TC A HA +++ I+DE+ P ++P + +F+L GD QL P++++ A GL +SLF L E + S C L Q+RM+ + K ++ YN Q + + V+ A E E FW +L + +P E+D Y N E + +E+VE + G I ++ P+ +Q+ I + S +++ + + ++ QG ++++S V + + F L D+ ++NV ++RA+ L+++GN YLS N +++HD
Sbjct: 2529 VDIIICTCAEAGSQRMRTHANIAQ-----CIVDESNYCLEPEVLVPAVTAGPRCSRFVLVGDHKQLVPVLQSKEAWKLGLGRSLFEDLWEAHREGSSACCTLNTQHRMHPSLGKFSSSFFYNVQQS--RENVSNACARELEG-----FWPA---GRDLYHVFCHVEDEP---ESDCDYIEGGMSNDAEVQKVVEVVESLMSRGVQPFKIWIVSPFLRQRHSILHALDSTLGSQLDIRLTSPEECQGAQCDVLVLSTVRGRPRDQLTGPHGGFGCLGDKHQMNVVLTRARKGLVIVGNRYYLSGNPMWNRLLHD 2823
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Nematostella
Match: EDO34933 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMW5]) HSP 1 Score: 63.929 bits (154), Expect = 3.676e-10 Identity = 80/310 (25.81%), Postives = 130/310 (41.94%), Query Frame = 1
Query: 2464 LIENIDVVGVTCLSANHAALSR-------LKFDVVIIDEAGQITLPVSIMPLLKMDQNKG-----KFILTGDLLQLPPLVRN-STARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTME----------VEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLS 3324
L++ ++ +TC HAAL R K+D V+++E+ QI + +PLL + G + L GD QLPP+++N + + + ++QSLF++ P +L Q R LCN YN + + T E+ N + Q + V P F Y N EA+ + L IG I ++ YN QK +IR++ ++ T+D+YQG+ +I+S V + L D RRL VA+SRA+ L ++ + S
Sbjct: 998 LVKEAKIIAMTC---THAALKRHDLVELGFKYDNVLMEESAQILEIETFIPLLLQNPEDGYNRLKRVCLIGDHNQLPPVIKNMAFQKFSNMEQSLFTRFVRLGVP--TVQLDAQGRARP---SLCNL--YNWRYKTLGNLPHVLTWPEYGVENPGFHFDFQLIDVQDFNGVGESEPNPYF------YQNLGEAEYAVALFMYMRLIGIPAERIAILTTYNGQKHLIRDVINQRCGNNPLIGRPSKVTTVDRYQGQQNDFIILSLV----------RTRTVGHLRDVRRLVVAMSRARLGLYILARVSLFS 1281
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Medaka
Match: dna2 (DNA replication helicase/nuclease 2 [Source:NCBI gene;Acc:101165382]) HSP 1 Score: 427.557 bits (1098), Expect = 4.672e-128 Identity = 317/1007 (31.48%), Postives = 506/1007 (50.25%), Query Frame = 1
Query: 610 TLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSK-KPEISKMQVI----------------------DVEENIWSTKYGIKGKIDVTLQTN-----------ESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVIST---IKIRNELTNFILKFT--SSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSR-LSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRN------------VTFESSSLSGINFLIKFNLPQKPFY--KFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKH----IMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYI---LPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFA--TIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPS---------FVEADEIY-----FNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQ----KEMIRNMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 3387
TL C +K DG WE T V GD+ + + G W + +G+LV+ P L+S TS++ S+ C R+ VL F G S K M+ G+ +H +FQ + + ++ ++A P L Y G + EE+ +E+ Y P + W + LS KP+ ++++ D+EEN+WS ++G+KGKIDVT Q E T PLELKTGR + S EH QV+LY LM ++ N + GLLLY+K G+ + +PS + +K+RN L + + + L+N LP ++T+ + CQ C C ++ R L + +D S ++D ++ GHL ++ +F HW+ L +LE ++ + +N K+I +Q+ + S +N V ++ + F + P + GD I+VS DG+ V + GY+ E T + S + L + R+D + + ++L+ L LM+ + ++LR IV +PP F +++ L K I+K N Q++ + + SKDY LI G PG+GKT+ I ++RI G VL++SYTHSAV NIL KL + FL L +K+ L Y + +K L + L VV TC+ H +R +FD I+DEA QI+ P+ + PL +F+L GD QLPP+V+N AR G+D+SLF +L H +++ +L +QYRMN +I+ L N++ Y+ ++ C +++ A A T+ S+ S E + D S + PS V A E N EA + L+ +K G SD+GVI PY QQ + ++++ + VE+NT+D+YQGRDKS++++SFV + + G E +L D RRLNVAI+RAKHKL+M+G+ L + + P++ + + L+ +IS
Sbjct: 372 TLHPTETCLLK---DG-WETTPVRRGDVVHL-EGRSDAGTWLVERDQGLLVLQPDSLISGTSISNSIRCMRRAVLGEMFKSFDGGS-KQMLNGTMVHEVFQGAAKAKDFSSEKLFKLADEALHSPRYLGDMYTLGVSQEEMKQELLDYLPSLENWASEYLSSSKPKSIGLKILNPSRAPGGCQESNAVVTVTDLTDIEENVWSPRFGLKGKIDVTAQVQIQRPRTSRSPEEKTFPLELKTGRESNSIEHRSQVILYTLMSMER--YNPEAGLLLYLKTGSMHPV----APSHMDRRELLKLRNTLAHHLHNSVEKGADLSNLQRLPDILTSRQTCQYCPQKLNCALYERALDPSSADPSDDVRDFLQQECGHLAQQHLNYFSHWLLLCILEAVTMET-KNSRKHIWLQSIESSEKNGSCVGNLQLKDAVAAQAEDVFLHRFQRTGSAPSQSATNCGLTNGDRIVVSDQDGRLVGLATGYLCEVDKTSVSCTLS-RDLSKFRDVLFRLDADEGVVGMSTHLTNLSRLMENCQDSERLRKLIVDLRPPEFIFNLSSVLPRDAKETVAEILKGLNKPQKQAMKKVLLSKDYTLIVGMPGTGKTTTICTLVRILQACGFSVLLTSYTHSAVDNILLKLKRFRVGFLRLGQG----QKIHPDILPYTEENVRKKGVHTLLDLEQLYNKELVVATTCMGIKHPIFTRRRFDYCIVDEASQISQPICLGPLFYAH----RFVLVGDHQQLPPIVQNKEARSLGMDESLFKRL--EVHGEAVVQLNVQYRMNRQIMSLSNSLMYDGRLECGSERTAAALLTLPSLASVQS-ELRSHSETHPQHDLSWVQFTLLPSNPVCFLDCSLVPALESVEQGGVSNHTEAALIHMLLSLLIKAGCRPSDVGVIAPYRQQLKTIQALLQSPTFSGVEVNTVDKYQGRDKSLIVLSFVRSNTEEGHLGE-----LLKDWRRLNVAITRAKHKLLMVGSAVTL-RRFPPVEKLLNHLQQENMISF 1347
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Medaka
Match: UPF1 (UPF1 RNA helicase and ATPase [Source:NCBI gene;Acc:101168695]) HSP 1 Score: 123.25 bits (308), Expect = 6.340e-28 Identity = 153/583 (26.24%), Postives = 255/583 (43.74%), Query Frame = 1
Query: 1759 LSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLK-IPISNLSVLGSLMDKSEIGQKLRDFIVHCKPP-------LFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMG-MKVLVSSYTHSAVSNILQKLD------------ARE-----INFLYLNGNHS---------VCEKLKNRTLSYI-LPEKKFENLFNF--HNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQI---TCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKE-MIRNMS---------TMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPI 3342
+ G +++ P +K + G I V + G ++AI L V +P + I + + K T + S+LK + SV G + K +G ++ D + C+ P L D + ++ AVK + + LI+G PG+GKT ++ G VLV + ++ AV + +K+D +RE ++FL L+ S ++LK+ T EK++ L L+ N DV+ TC+ A L++++F ++IDE+ Q T P ++P++ + IL GD QL P+V A GL QSLF +L P L++QYRM+ + + I Y + A D++ +F W + D+ + F Q + Y N+ EA ++ + LK GA IG+I PY Q+ +++ M +VEI ++D +QGR+K +I+S V +A E+ LND RRLNVA++RAK+ +I++GN LS+ QP+
Sbjct: 338 MQGDEICLRYKGDLAPLWKGI-GHVIKVPDNYGDEIAIELRSSVGAP---VEIPHNFQVDFVWKSTSFD-RMQSALKTFAVDETSVSGYIYHKL-LGHEVEDVTIKCQLPKRFTAQGLPDLNHSQVYAVKT------------------VLQRPLSLIQGPPGTGKTVTSATIVYHLSRQGNGPVLVCAPSNIAVDQLTEKIDKTGLKVVRLCAKSREAIESPVSFLALHNQISNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVL---GAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRP---IRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRIK----------KGFDFQW------PQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQQVEIASVDAFQGREKDFIILSCV-------RANEHQGIGFLNDPRRLNVALTRAKYGVIIVGNPKALSK--QPL 865
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Medaka
Match: ighmbp2 (immunoglobulin mu DNA binding protein 2 [Source:NCBI gene;Acc:101158519]) HSP 1 Score: 108.227 bits (269), Expect = 2.221e-23 Identity = 110/455 (24.18%), Postives = 190/455 (41.76%), Query Frame = 1
Query: 2158 QRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLF----------------------NFHNLIENI-------DVVGVT--------CLSANHAA--------LSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINS--LEFWKCQCCSSELDRSVIFLNYQPSFVEADEIY-----FNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTM---EVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHD 3357
QR +M A+ ++ +I G PG+GKT+ +V +I V G KVL + ++ AV N++++L + L L + E ++ +L IL + N+ N+ I + + +T LS N A L FD V+IDE Q + LLK K IL GD QLPP +++ A GL SL ++ + + L +QYRMN I++ + Y ++T H S+ S L+ +E ++ ++ S + E+ N+ E I ++ + G DI VI PYN Q +++R + E+EI ++D +QGR + L ++ L++ RR+NVA++RA+ + ++ + + QN+ +K + D
Sbjct: 194 QREAVMFALSQRELAVIHGPPGTGKTTTVVEIILQAVKQGQKVLCCAPSNVAVDNLVERLAQCKAKVLRLGHPARLLESIQKHSLDAILAQSDNANIIADIRKDIDKVLMGMKKEKGERGNYKREIGELKKELKSREATAITQILKGADVVLSTNTGACDDGPLKLLPAEHFDWVVIDECAQALESSCWIALLKAR----KCILAGDYKQLPPTIKSQAAASKGLSLSLMERVIQIYGDVVVRMLTVQYRMNSAIMEWASKEMYQGKLTA------------HCSVESHLLKDLTGVASVAETSTPLLLIDTAGSGLTEMEVTDEQSKGNQGEVDIVELHIKALTEAGVKAKDIAVIAPYNLQVDLLRQRLSARHPELEIKSVDGFQGR-------EKEAVVLSLVRSNRKGEVGFLSEDRRINVAVTRARRHIAVVCDTQTV-QNHAFLKSLVD 624
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Medaka
Match: ENSORLT00000003680.2 (Mov10 like RISC complex RNA helicase 1 [Source:NCBI gene;Acc:101169938]) HSP 1 Score: 93.2041 bits (230), Expect = 1.124e-18 Identity = 107/436 (24.54%), Postives = 213/436 (48.85%), Query Frame = 1
Query: 2107 KSLAVKIKHIMKICNPDQR----RVIMSAIFSKDYLLIKGYPGSGKTSCIVAMI-RIF-VLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLK--NRTLSYILP--EKKFENLFN--FHNLIENIDVVGVTCLSA---NHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCE---FNHPDSICELKL------QYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVE--ADEIYFNKFEAKITM----ELVEQFLK-IGALESDIGVIIPYNQQKEMIR----NMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGN 3309
+++ V + NP QR R++ Y+L G PG+GKT ++ +I +++ L ++LV + ++SA I +L FL NG+ + N +L +L K E++ + FH ++ + TC SA ++ + +F + +DEAGQ T P S++P+ + + G+ +L GD QL P+V++ A + GL SL +L ++ DS + KL YR + +L L + + Y+ +++ + ++ E S+ F ++F + + + ++ +FN EA M +L ++ K + A +DIG+I PY +Q E IR + ++++ +++++QG++ ++I+S V ++ + + L++ +R NVA++R+K L+++GN
Sbjct: 731 RAIPVSGHFFNPVLNPAQREAVKRILAGECRPIPYVLF-GPPGTGKTITLIEVILQVYHFLPNSRILVCTPSNSAADLICTRLHYS--GFLE-NGSLARVNAFSRDNESLPEVLRPYAKAGEDIRHAAFHRIVVS------TCSSAGMFHNIGIRVGQFSHLFLDEAGQATEPESMIPISLISEKDGQIVLAGDPCQLGPVVKSEVASVFGLGVSLLERLMTNPLYSRQDSGYDPKLVTKLIYNYRSHEVLLSLPSKLFYHGELSFQAPRSVVDSLSEWNSLPKKGF------------PLLFHGVRGTEMREGSNPSWFNPVEAVQVMMYCCQLAKKLYKPVNA--ADIGIIAPYRKQCEKIRVLLGKVGLQDIKVGSVEEFQGQEYLVIILSTVRSNESVQSNDLQSLLGFLSNPKRFNVAVTRSKALLLIVGN 1142
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Medaka
Match: ENSORLT00000034865.1 (putative helicase mov-10-B.2 [Source:NCBI gene;Acc:101175667]) HSP 1 Score: 91.6633 bits (226), Expect = 2.749e-18 Identity = 125/490 (25.51%), Postives = 221/490 (45.10%), Query Frame = 1
Query: 2047 QKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIV-AMIRIFVLMGMKVLVSSYTHSAVSNILQKL-----DAREINFLY--LNGNHSVCEKLK------NRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKM-DQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-----HPD------SICELKLQYRMNIEILKLCNTISYNNQI-TCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLK--IGALE---------SDIGVIIPYNQQKEMIRNMSTME----------VEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRAN 3372
++L + +PP F+ + + AV HI+ RR A + L+ G PG+GKT +V A++ I + +LV + ++SA +I +K+ D ++ LY L+ + E LK + + +P KK ++ + L+ + G + + + +DEAGQ T S++P+ + D++ + +L GD QL P+V + TA +GL SL +L N H D I +L YR + ILK+ N + Y ++ CA + N LE + V+F + + + E F+ F +E+++++LK I L +IG+I PY +Q E I+ + VE+ T+D++QG++ S++++S V KL K +N + D RR NVA++RA+ L+++G+ L+ + IH L+A
Sbjct: 378 RRLPRLLALNRPPRFEFNPEQQKAVT--HIL-------RRTAKPAAY-----LLCGPPGTGKTLTLVKAIMEIVKAQDINILVCAPSNSASDDICEKILKGLVDTDKVYRLYPLLHSIAKIPEILKKNCNLDQKKGTIKIPNKK--SIMSRKILVTTLQTAGRLVT----GGIPPKHYTYIFVDEAGQATETESMIPIAGLCDRSTCQVVLAGDPKQLGPVVISKTAEYHGLGVSLLERLMRDNDLYKPHEDFGFDSNFITKLLKNYRSHPAILKVPNDLFYGGELQPCARLSKLY---------NQLELLPKEGF------PVVF--HGVAGISQQEKGFSSFYNMAEIEVIKEYLKSLIQHLHRNNVSHVPPGEIGIISPYRKQVEKIQKAIQWDADLSKENLENVEVGTVDKFQGKEFSVILMSTVRCFPKL-KHKQNFILGFIRDDRRFNVAMTRARSLLVVVGDPRLLATDKNWNTFIHYCLKAG 829
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Planmine SMEST
Match: SMESG000005730.1 (SMESG000005730.1) HSP 1 Score: 2160.57 bits (5597), Expect = 0.000e+0 Identity = 1107/1115 (99.28%), Postives = 1111/1115 (99.64%), Query Frame = 1
Query: 43 MKQTNIFDFVKKIDQNEIENQKKLLNENVKPLCPKSINFLRKRKKSHLVDGRNYSSNQVVLSKDLNSNQIDSSIVSNKLSSYQDRXXXXXXXXXXXXXXVESDNKENIYSNRIPIPTFKAVLNEYNNKFDENNKKQLFDDCFNDDFKWDESLIEDKIKQISPNKIQDQDFYYXXXXXXXXXXXXKQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQVIDVEENIWSTKYGIKGKIDVTLQTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESSSLSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 3387
MKQTNIFDFVKKIDQNEIENQKKLLNENVKPLCPKS NFLRKRKKSHLVDGRNYSSNQVVLSKDLNSNQIDSSIVSNKLSSYQDRIKKINDKLNIKKTIV+SDNKENIYSNRIPIPTFKAVLNEYNNKFDENNKKQLFDDCFNDDFKWDESLIEDKIKQISPNKIQDQDFYYCVVNNVESVCNVKQLTVTL+DNYKCQVKLHLDGMWEDTVVHV DICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQVID+EENIWSTKYGIKGKIDVTLQTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESSS SGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSL+FWK QCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTMEVEINTIDQYQGRDKSIVIISFVSKSDKLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI
Sbjct: 1 MKQTNIFDFVKKIDQNEIENQKKLLNENVKPLCPKSTNFLRKRKKSHLVDGRNYSSNQVVLSKDLNSNQIDSSIVSNKLSSYQDRIKKINDKLNIKKTIVDSDNKENIYSNRIPIPTFKAVLNEYNNKFDENNKKQLFDDCFNDDFKWDESLIEDKIKQISPNKIQDQDFYYCVVNNVESVCNVKQLTVTLQDNYKCQVKLHLDGMWEDTVVHVRDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLSKKPEISKMQVIDIEENIWSTKYGIKGKIDVTLQTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQLTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKKSIRNVTFESSSHSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKIKHIMKICNPDQRRVIMSAIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLKFWKRQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTMEVEINTIDQYQGRDKSIVIISFVSKSDKLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 1115
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Planmine SMEST
Match: SMESG000052537.1 (SMESG000052537.1) HSP 1 Score: 961.444 bits (2484), Expect = 0.000e+0 Identity = 495/988 (50.10%), Postives = 697/988 (70.55%), Query Frame = 1
Query: 514 KQISPNKIQDQDFYYXXXXXXXXX-XXXKQLTVTLEDNYKCQVKLHLDGMWEDTVVHVGDICSIVDPIKSNGLWTINNHRGILVVNPFYLVSTTSVTGSLFCQRKVVLQHFFSGVSGQSNKAMVIGSFIHLIFQECCRLEHLDLNEVREIAKRLSKQPSQLITFYESGWTDEEILREIEVYFPRILKWINRNLS-----KKPEISKMQVIDVEENIWSTKYGIKGKIDVTLQTNESTIPLELKTGRTTFSREHTCQVLLYILMMSDKTEMNIDKGLLLYIKDGAERFIFNDNSPSVISTIKIRNELTNFILKFTSSQ---LTNADCLPSVITNIRLCQSCEYLSLCTMFSRLSQNDSDCSQAMKDLIKDNTGHLCEKDYEFFVHWINLIVLEFLSESSNENPIKNIIVQNSKK---SIRNVTFESSSLSGINFLIKFNLPQKPFYKFVEGDPIIVSSDDGKKVAIVLGYVVESPGTDILIISSEKPLICSKMTKLRIDLNSSLKIPISNLSVLGSLMDKSEIGQKLRDFIVHCKPPLFDCHITKSLAVKI-------------KHIMKICNPDQRRVIMSAI-FSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATI-MEHESINSLEFWKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMIRNMSTMEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPE---NNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPIKVIHDVLRANQIISI 3387
+ I+P IQ QDFY VV ++S +K+LT+ +N Q+KL+L+G+W + +++ +IC++++PIK+ W INN++G++V+NP +L+++T++TGSLFCQRK V QH+F+ +SG+S K+MV+G+FIH IFQ+C + ++L ++++++I K Q Q+++FYES ++D EI+ E+E+YFP+IL+WI RN+S K+ + K +ID+EE+IWS KYG+KGKID+TLQ+ ES +PLELKTG+T+FSR+HT QVLLY LMMSD + +ID GLLLYIKDG+E+ + N+ P+VIS + +RNEL N++ +F ++ + + LP V+ N R C CEYL+LC+ FSRL+ +++ + M+ LI+ NT HL KD++F WINLI++E+ SE S E IKNI ++ SIRNV F+SS +G F + F + K EGDP+IVSSDDGK++AI G++ S D L I S+KPL CS KLRID NS +KIPISNLS L +LMD+SE Q LR +I+H P H ++ V I K I+K CN DQ+ IM + S + LIKGYPGSGKT+C+VAMIR+FV +GM+VLVSSYTHSAVSNIL+KL +++F+YL+ +H VC ++ +RTL + E + +E I+V+GVTCLSA+H LSRLKFD+V+IDEAGQITLP+S+MPLLKM G F+L GD+ QLPPLV+NSTAR++GL+QSLF LC NH ++I EL +QYRMN EIL+LCN I+Y N+I+CA++ VA AT M+ + E WK QC S+EL SV+F+N+Q + +EIYFN+FEA++ EL +FL +G ES IG+I PYN QK+++ T +EINTIDQ+QG+DKSI+IIS V + A E N S +IL D RR+NVA++R+KHKLI++GNLDYL + Y+PI + +VLR +QI+SI
Sbjct: 81 ENINPEIIQIQDFYRVVVIEIDSRDETIKRLTLKTCENNGYQIKLNLEGLWANCLINENEICNVINPIKTKENWKINNNQGLIVINPDFLLNSTAITGSLFCQRKSVFQHYFTDISGKSKKSMVLGTFIHSIFQKCSKEQNLSIDKIKKITKNFLIQEKQMLSFYESEFSDNEIMIELEIYFPQILQWIYRNISQSEHDKRINMKKSSIIDIEESIWSVKYGMKGKIDMTLQSGESIVPLELKTGKTSFSRDHTSQVLLYTLMMSDARQTDIDSGLLLYIKDGSEKPVINNRHPNVISIVMLRNELANYLSRFLTNPPPVANDTNLLPPVLENNRFCGQCEYLTLCSTFSRLTNENTN--ETMEALIQANTDHLSGKDFQFLTKWINLILVEYFSERSGEKIIKNISTNSNNAVSDSIRNVVFDSSKFTGNQFQLTFVFEENKSIKLTEGDPVIVSSDDGKRLAIATGFICGSISIDRLSILSDKPLPCSSALKLRIDRNSYMKIPISNLSALANLMDRSETSQSLRRYIIHGDNPAL--HTVANVPVAIGNQYEQSIYKKIDKSILKHCNQDQKSAIMRTVGLSSGFSLIKGYPGSGKTACVVAMIRVFVQLGMRVLVSSYTHSAVSNILEKLIDCQVDFIYLSASHKVCNRISDRTLGSFISRNAIETVSQLEKALERINVIGVTCLSAHHPLLSRLKFDIVMIDEAGQITLPISLMPLLKMKPTTGVFLLAGDVHQLPPLVKNSTARLDGLNQSLFEYLCNHNHDNTISELTIQYRMNEEILRLCNEITYGNRISCASEMVAKATFAMDTKLKGERESWKRQCLSNELRHSVVFVNHQKK--QPNEIYFNQFEAQLVTELANEFLDLGVSESVIGIIAPYNAQKDLLIEFRTGGIEINTIDQFQGKDKSIIIISLVRQQSTNQTAREVADNESLDILKDNRRINVALTRSKHKLILVGNLDYLPEFYRPIAELRNVLRPDQIVSI 1062
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Planmine SMEST
Match: SMESG000052629.1 (SMESG000052629.1) HSP 1 Score: 199.519 bits (506), Expect = 5.874e-58 Identity = 110/263 (41.83%), Postives = 153/263 (58.17%), Query Frame = 1
Query: 2251 MIRIFVLMGMKVLVSSYTHSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFL 3036
MIR+FV +GM VLVSSY HSAVSNIL+KL +++F+YL+G+H VC ++ +RT + + E + +E I+V+GVT LSA+H LSR+KFD+V+IDEAGQITLP+S+MPLLKM K L QYRMN +IL+LCN I+Y+N+I+C ++ VA A + + WK QC S EL SV+F+N+Q + +IYFN+ EA++ MEL +FL
Sbjct: 1 MIRVFVQLGMWVLVSSYMHSAVSNILEKLIDCQVDFIYLSGSHKVCYRISDRTFTSFISRNAIETVSQLEKAMERINVIGVTSLSAHHPLLSRVKFDIVMIDEAGQITLPISLMPLLKMKPTK---------------------------------------------LTNQYRMNEDILRLCNEITYDNRISCPSEMVAKAKFAMNPKLKCERVSWKRQCLSKELRHSVVFVNHQKK--QPSKIYFNQLEAQLAMELANEFL 216
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Planmine SMEST
Match: SMESG000052629.1 (SMESG000052629.1) HSP 1 Score: 174.481 bits (441), Expect = 1.937e-49 Identity = 97/245 (39.59%), Postives = 138/245 (56.33%), Query Frame = 1
Query: 2305 HSAVSNILQKLDAREINFLYLNGNHSVCEKLKNRTLSYILPEKKFENLFNFHNLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFNHPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFLNYQPSFVEADEIYFNKFEAKITMELVEQFL 3036
HSAVSNIL+KL +++F+YL+G+H VC ++ +RT + + E + +E I+V+GVT LSA+H LSR+KFD+V+IDEAGQITLP+S+MPLLKM K L QYRMN +IL+LCN I+Y+N+I+C ++ VA A + + WK QC S EL SV+F+N+Q + +IYFN+ EA++ MEL +FL
Sbjct: 2 HSAVSNILEKLIDCQVDFIYLSGSHKVCYRISDRTFTSFISRNAIETVSQLEKAMERINVIGVTSLSAHHPLLSRVKFDIVMIDEAGQITLPISLMPLLKMKPTK---------------------------------------------LTNQYRMNEDILRLCNEITYDNRISCPSEMVAKAKFAMNPKLKCERVSWKRQCLSKELRHSVVFVNHQKK--QPSKIYFNQLEAQLAMELANEFL 199
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Planmine SMEST
Match: SMESG000077362.1 (SMESG000077362.1) HSP 1 Score: 118.242 bits (295), Expect = 1.891e-26 Identity = 118/432 (27.31%), Postives = 197/432 (45.60%), Query Frame = 1
Query: 2179 AIFSKDYLLIKGYPGSGKTSCIVAMIRIFVLMGM-KVLVSSYTHSAVSNILQKLD------------------------AREINFLYLNGNHSV--CEKLKNRTLSYILPEKKFENLFNFH---NLIENIDVVGVTCLSANHAALSRLKFDVVIIDEAGQITLPVSIMPLLKMDQNKGKFILTGDLLQLPPLVRNSTARINGLDQSLFSKLCEFN-HPDSICELKLQYRMNIEILKLCNTISYNNQITCATDQVAFATIMEHESINSLEF-WKCQCCSSELDRSVIFL--NYQPSFVEADEIYFNKFEAKITMELVEQFLKIGALESDIGVIIPYNQQKEMI-------RNMST---MEVEINTIDQYQGRXXXXXXXXXXXXXXXLGKAPENNSFNILNDQRRLNVAISRAKHKLIMIGNLDYLSQNYQPI 3342
+ + LI+G PG+GKT ++ M +VLV + ++ AV + +K+D A + ++GN ++LK+ T I +++ + +L+ N DV+ TC+ A LS+++F V+IDE+ Q T P ++ L+ + + +L GD QL P+V A GL QSLF +L P L++QYRM+ + + I Y + + E N ++F W D+ + F + Q Y N+ EA +LV + LK G +IGVI PY Q+ + ++T E+E+ ++D +QGR+K +++S V +A E+ LND RRLNVA++RAK+ LI++GN L++ QP+
Sbjct: 455 TVLQRPLSLIQGPPGTGKTVTSATIVYHLSQMNHGQVLVCAPSNIAVDQLTEKIDKIGLKVVRLCAKSREAIQSPVSHLALHVQVQNMDGNSEFKKLQELKDVTGELIQSDERRYRMLKKQLERDLLMNADVICCTCVGAGDPRLSKMRFPCVLIDESTQATEPECLISLVLGCK---QAVLVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRP---MRLQVQYRMHPVLSAFPSNIFYEGSLQNG--------VTAEERNNKIDFPW------PNPDKPMYFYCSSGQEEMASNGTSYLNRTEAATVEKLVTRLLKDGVKPCNIGVITPYEGQRAYLVHYFHFSGQLNTKLYQEIEVASVDAFQGREKDYILLSCV-------RANEHQGIGFLNDPRRLNVALTRAKYGLIIVGNPKVLAK--QPL 857 The following BLAST results are available for this feature:
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 4
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA replication ATP-dependent helicase/nuclease DNA2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014833 ID=SMED30014833|Name=DNA replication ATP-dependent helicase/nuclease DNA2|organism=Schmidtea mediterranea sexual|type=transcript|length=3605bpback to top protein sequence of SMED30014833-orf-1 >SMED30014833-orf-1 ID=SMED30014833-orf-1|Name=SMED30014833-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1116bp MKQTNIFDFVKKIDQNEIENQKKLLNENVKPLCPKSINFLRKRKKSHLVDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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