Calcium-activated potassium channel slo-1, putative
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014811 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 370/1078 (34.32%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 75 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1119
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 370/1078 (34.32%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 75 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1119
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 370/1078 (34.32%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 75 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1119
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284]) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 370/1078 (34.32%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 75 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1119
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:HGNC Symbol;Acc:HGNC:6284]) HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0 Identity = 370/1081 (34.23%), Postives = 614/1081 (56.80%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDS---VSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K + +K +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 75 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1123
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25]) HSP 1 Score: 634.795 bits (1636), Expect = 0.000e+0 Identity = 383/1105 (34.66%), Postives = 609/1105 (55.11%), Query Frame = 2
Query: 377 EMNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLK-----ISQSTNFETACRSQKNLESLIEDP----------SFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTN-PIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK-------------------------------GSPDDY-----ELTVISRLKSEEEVSLTKCDVENDFKEMEFIDE-----KCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSE--TQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVS-QINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
E + +C +RK++ F+ + ++L+VIW+++ + ++R K I + K+ S DP + + + +LIS Q L GR + + + ++ S ++Y Y+ ++E C P + P DL N FL+YF +RF +++K+ F L ++ ID FTIP A+ L + W+G RF+R L L+ V +++Q N++K+ +SIR+ QL+T F ++ + GAG HLLENSG+ G + N I Y DS+YF++VTMSTVGYGDI+ T+CG++F++ FIL LA FAS++PE+A+ + +Y G Y G++HI+VCGHI Y SVS FL DFLHEDR DV+V+F+++ PD+E++ L K +FT+V++F GTVMD +DL RV++ +ADACL+LA++ ++P+ EDA NIMR IS KNY VI+QL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P WLNDY+RG ME+Y+ LS SF S+ E F L LLL+AIE+KD +K+ + +NP I P + GFFI S + A +C +CH ++D++ +K C CK P + +L +I++ + + D + ++F + + Y G F+ C P + +++ + HQ + L GH+++C+F+D + IG+RNF+MPLRS +ELK +VI+ D +++ KEW + PKI ++ R ++I+ C CVI++ + + D + DKE I+A+L +A M+ + + G +T G+ KG++ T VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+N +A+ ++ L+ GG+ +L+ + T L R+ R + +S Q N + Y +F + ++ + +GLYR L + +++ RYVITNPP I+ TD VY L+Q
Sbjct: 34 EEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSET-DPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKG--FINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWC-PSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTE---DTTLADKEAILASLNIKA------MQFDDTL-----------GFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRD---RCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYR----LHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQ 1104
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25]) HSP 1 Score: 627.476 bits (1617), Expect = 0.000e+0 Identity = 381/1105 (34.48%), Postives = 609/1105 (55.11%), Query Frame = 2
Query: 377 EMNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLK-----ISQSTNFETACRSQKNLESLIEDP----------SFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTN-PIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK-------------------------------GSPDDY-----ELTVISRLKSEEEVSLTKCDVENDFKEMEFIDE-----KCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSE--TQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVS-QINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
E + +C +RK++ F+ + ++L+VIW+++ + ++R K I + K+ S DP + + + +LIS Q L GR + + + ++ S ++Y Y+ ++E C P + P DL N FL+YF +RF +++K+ F L ++ ID FTIP A+ L + W+G RF+R L L+ V +++Q N++K+ +SIR+ QL+T F ++ + GAG HLLENSG+ G + N I Y DS+YF++VTMSTVGYGDI+ T+CG++F++ FIL LA FAS++PE+A+ + +Y G Y G++HI+VCGHI Y SVS FL DFLHEDR DV+V+F+++ PD+E++ L K +FT+V++F GTVMD +DL RV++ +ADACL+LA++ ++P+ EDA NIMR IS KNY VI+QL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K + P WLN Y+ G ME+Y+ LS SF ++ E V+ F L LLL+AIE+KD +K+ + +NP I P + GFFI S + A +C +CH ++D++ +K C CK P + +L +I++ + + D + ++F + + Y G F+ C P + +++ + HQ + L GH+++C+F+D + IG+RNF+MPLRS +ELK +VI+ D +++ KEW + PKI ++ R ++I+ C CVI++ + + D + DKE I+A+L +A M+ + + G +T G+ KG++ T VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+N +A+ ++ L+ GG+ +L+ + T L R+ R + +S Q N + Y +F + ++ + +GLYR L + +++ RYVITNPP I+ TD VY L+Q
Sbjct: 34 EEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSET-DPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKG--FINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRSFKTSQTTP---DWLNLYLCGAGMEMYTDTLSHSFVGMTFPEAVDLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCKNLALFRRNTKHSTAARARATDVLQQFQPQAPAGPMGHLGQQVQLRMINQQRPSSGGRRNSMSIPPDGRGVDFSKDFEQQFQDMKYDSTGMFHWC-PSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTE---DTTLADKEAILASLNIKA------MQFDDTL-----------GFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRD---RCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYR----LHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQ 1104
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25]) HSP 1 Score: 488.034 bits (1255), Expect = 2.861e-152 Identity = 259/635 (40.79%), Postives = 395/635 (62.20%), Query Frame = 2
Query: 377 EMNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLK-----ISQSTNFETACRSQKNLESLIEDP----------SFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTN-PIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK 2233
E + +C +RK++ F+ + ++L+VIW+++ + ++R K I + K+ S DP + + + +LIS Q L GR + + + ++ S ++Y Y+ ++E C P + P DL N FL+YF +RF +++K+ F L ++ ID FTIP A+ L + W+G RF+R L L+ V +++Q N++K+ +SIR+ QL+T F ++ + GAG HLLENSG+ G + N I Y DS+YF++VTMSTVGYGDI+ T+CG++F++ FIL LA FAS++PE+A+ + +Y G Y G++HI+VCGHI Y SVS FL DFLHEDR DV+V+F+++ PD+E++ L K +FT+V++F GTVMD +DL RV++ +ADACL+LA++ ++P+ EDA NIMR IS KNY VI+QL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P WLNDY+RG ME+Y+ LS SF S+ E F L LLL+AIE+KD +K+ + +NP I P + GFFI S + A +C +CH ++D++ +K C CK
Sbjct: 34 EEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSET-DPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKG--FINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCK 662 HSP 2 Score: 177.178 bits (448), Expect = 1.120e-44 Identity = 126/404 (31.19%), Postives = 205/404 (50.74%), Query Frame = 2
Query: 2309 DVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSE--TQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVS-QINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
D DF E +F D K Y G F+ C P + +++ + HQ + L GH+++C+F+D + IG+RNF+MPLRS +ELK +VI+ D +++ KEW + PKI ++ R ++I+ C CVI++ + + D + DKE I+A+L +A M+ + + G +T G+ KG++ T VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+N +A+ ++ L+ GG+ +L+ + T L R+ R + +S Q N + Y +F + ++ + +GLYR L + +++ RYVITNPP I+ TD VY L+Q
Sbjct: 746 DFSKDF-EQQFQDMK---YDSTGMFHWC-PSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTE---DTTLADKEAILASLNIKA------MQFDDTL-----------GFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRD---RCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYR----LHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQ 1117
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25]) HSP 1 Score: 488.419 bits (1256), Expect = 2.894e-152 Identity = 259/635 (40.79%), Postives = 395/635 (62.20%), Query Frame = 2
Query: 377 EMNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLK-----ISQSTNFETACRSQKNLESLIEDP----------SFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTN-PIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK 2233
E + +C +RK++ F+ + ++L+VIW+++ + ++R K I + K+ S DP + + + +LIS Q L GR + + + ++ S ++Y Y+ ++E C P + P DL N FL+YF +RF +++K+ F L ++ ID FTIP A+ L + W+G RF+R L L+ V +++Q N++K+ +SIR+ QL+T F ++ + GAG HLLENSG+ G + N I Y DS+YF++VTMSTVGYGDI+ T+CG++F++ FIL LA FAS++PE+A+ + +Y G Y G++HI+VCGHI Y SVS FL DFLHEDR DV+V+F+++ PD+E++ L K +FT+V++F GTVMD +DL RV++ +ADACL+LA++ ++P+ EDA NIMR IS KNY VI+QL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P WLNDY+RG ME+Y+ LS SF S+ E F L LLL+AIE+KD +K+ + +NP I P + GFFI S + A +C +CH ++D++ +K C CK
Sbjct: 34 EEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSET-DPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKG--FINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCK 662 HSP 2 Score: 177.178 bits (448), Expect = 1.159e-44 Identity = 126/404 (31.19%), Postives = 205/404 (50.74%), Query Frame = 2
Query: 2309 DVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSE--TQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVS-QINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
D DF E +F D K Y G F+ C P + +++ + HQ + L GH+++C+F+D + IG+RNF+MPLRS +ELK +VI+ D +++ KEW + PKI ++ R ++I+ C CVI++ + + D + DKE I+A+L +A M+ + + G +T G+ KG++ T VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+N +A+ ++ L+ GG+ +L+ + T L R+ R + +S Q N + Y +F + ++ + +GLYR L + +++ RYVITNPP I+ TD VY L+Q
Sbjct: 755 DFSKDF-EQQFQDMK---YDSTGMFHWC-PSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTE---DTTLADKEAILASLNIKA------MQFDDTL-----------GFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRD---RCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYR----LHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQ 1126
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Match: slo-1 (Calcium-activated potassium channel slo-1 [Source:UniProtKB/Swiss-Prot;Acc:Q95V25]) HSP 1 Score: 488.034 bits (1255), Expect = 3.435e-152 Identity = 259/635 (40.79%), Postives = 395/635 (62.20%), Query Frame = 2
Query: 377 EMNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLK-----ISQSTNFETACRSQKNLESLIEDP----------SFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTN-PIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK 2233
E + +C +RK++ F+ + ++L+VIW+++ + ++R K I + K+ S DP + + + +LIS Q L GR + + + ++ S ++Y Y+ ++E C P + P DL N FL+YF +RF +++K+ F L ++ ID FTIP A+ L + W+G RF+R L L+ V +++Q N++K+ +SIR+ QL+T F ++ + GAG HLLENSG+ G + N I Y DS+YF++VTMSTVGYGDI+ T+CG++F++ FIL LA FAS++PE+A+ + +Y G Y G++HI+VCGHI Y SVS FL DFLHEDR DV+V+F+++ PD+E++ L K +FT+V++F GTVMD +DL RV++ +ADACL+LA++ ++P+ EDA NIMR IS KNY VI+QL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P WLNDY+RG ME+Y+ LS SF S+ E F L LLL+AIE+KD +K+ + +NP I P + GFFI S + A +C +CH ++D++ +K C CK
Sbjct: 34 EEDRKCLEERKYWCFLLSSITTFCASMILVVIWRVVTHLCCQRREKEFVEPIPAPEAVQINMNGSKHAPSET-DPFLKQQEEKHLGWMTEAKDWAGELISGQSLTGRFLVLLVFILSIGSLIIYFYDASFQNFQVETCIPWQDSPSQQIDLGFNIFFLVYFFIRFIAASDKVWFLLEMYSWIDFFTIPPSFVAIYLQRNWLGFRFLRALRLMTVPDILQYLNILKTSSSIRLTQLVTIFVAVCLTGAGLVHLLENSGDFFKG--FINPHRITYADSVYFVLVTMSTVGYGDIYCTTLCGRLFMIFFILFGLAMFASYVPEIADLIGNRQKYGGEYKGEHGKKHIVVCGHITYDSVSHFLQDFLHEDRDDVDVEVVFLHRVVPDLELEGLFKRHFTKVEFFTGTVMDSLDLSRVKIGDADACLVLANKYSTNPDAEDAANIMRVISIKNYSSDIRVIVQLMQYHNKAYLLNIPSWDWKRGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPHTPLWLNDYLRGAGMEMYTESLSPSFANMSFPEAANLLFNRLGLLLLAIELKDEENKECNIAINPGPHIVIQPQTQGFFIAQSADEVKRAFFWCKQCHDDIKDVSLIKKCKCK 662 HSP 2 Score: 176.792 bits (447), Expect = 1.384e-44 Identity = 126/404 (31.19%), Postives = 205/404 (50.74%), Query Frame = 2
Query: 2309 DVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSE--TQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVS-QINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
D DF E +F D K Y G F+ C P + +++ + HQ + L GH+++C+F+D + IG+RNF+MPLRS +ELK +VI+ D +++ KEW + PKI ++ R ++I+ C CVI++ + + D + DKE I+A+L +A M+ + + G +T G+ KG++ T VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+N +A+ ++ L+ GG+ +L+ + T L R+ R + +S Q N + Y +F + ++ + +GLYR L + +++ RYVITNPP I+ TD VY L+Q
Sbjct: 753 DFSKDF-EQQFQDMK---YDSTGMFHWC-PSRNLEDCVLERHQAAMTVLNGHVVVCLFADQDSPLIGLRNFIMPLRSSNFHYHELKHVVIVGDLEYLRKEWKTLYNLPKISILNGSPLSRADLRAVNINLCDMCVIISARVPNTE---DTTLADKEAILASLNIKA------MQFDDTL-----------GFFPMRHQTGDRSPLGSPISMQKKGAKFGTNVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAISVLDSLMSTTYFNDSALTLIRTLVTGGATPELELILAEGAGLRGGYSTPETLSNRD---RCRIAQISLQDNPYDGVVHNTTYGAMFTIALRRYGQLCIGLYR----LHDQDNPDSMKRYVITNPPAELRIKNTDYVYVLEQ 1124
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0100624) HSP 1 Score: 520.39 bits (1339), Expect = 7.355e-164 Identity = 264/641 (41.19%), Postives = 402/641 (62.71%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDS-VSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTK 2305
+C RK++ F+ + GLL++++W+ RK + + + ++ +N + + +F + + +LIS Q GRI+ + + ++ S ++Y + ++E+C +N I DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q NV+K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN+ + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS IPE+ E + +G++Y G + G+RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++KEPD+E++ LLK ++T V +FQGT+M+ VDL+RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P +SW NDY+RG ME+Y+ LS +F + + E CF L LLL+AIE+K + + +NP K I + GFFI S + A YC CH ++D T +K C CK LTV R K ++ +T+
Sbjct: 42 DCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEF--EGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVD--ASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLD---FNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKEPDLELEGLLKRHYTTVAFFQGTMMNAVDLERVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAK-IQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCK------NLTVQPRSKFDDLGDITR 665 HSP 2 Score: 181.8 bits (460), Expect = 5.074e-46 Identity = 133/428 (31.07%), Postives = 215/428 (50.23%), Query Frame = 2
Query: 2291 VSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPS 3523
VSL ++ K+ +F ++ Y G F+ SP K +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C C IL+ K D D + DKE I+A+L +A + +I + + G E N S + G+ +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD V+ L Q G E+ PP+
Sbjct: 752 VSLPAGIADDQSKDFDF-EKTEMKYDSTGMFH-WSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSND---DPTLADKEAILASLNIKA------------MTFDDTIGVLSQRGP---EFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGK------YGDLFVAALKSYGMLCIGLYRFRD--TSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQ----FDPGLEYKPPA 1147
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0301582) HSP 1 Score: 519.62 bits (1337), Expect = 3.505e-163 Identity = 259/617 (41.98%), Postives = 390/617 (63.21%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDS-VSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK 2233
+C RK++ F+ + GLL++++W+ RK + + + ++ +N + + +F + + +LIS Q GRI+ + + ++ S ++Y + ++E+C +N I DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q NV+K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN+ + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS+IPE+ E S+Y G Y K +RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++KEPD+E++ LLK ++T V +FQGT+M+ VDL+RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P +SW NDY+RG ME+Y+ LS +F + + E CF L LLL+AIE+K + + +NP K I + GFFI S + A YC CH ++D T +K C CK
Sbjct: 25 DCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEF--EGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVDASSE--EVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLD---FNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAVFASWIPEITELAAQRSKYGGTYSKDPRKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKEPDLELEGLLKRHYTTVAFFQGTMMNAVDLERVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAK-IQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCK 630 HSP 2 Score: 181.03 bits (458), Expect = 1.101e-45 Identity = 133/428 (31.07%), Postives = 215/428 (50.23%), Query Frame = 2
Query: 2291 VSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPS 3523
VSL ++ K+ +F ++ Y G F+ SP K +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C C IL+ K D D + DKE I+A+L +A + +I + + G E N S + G+ +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD V+ L Q G E+ PP+
Sbjct: 787 VSLPAGIADDQSKDFDF-EKTEMKYDSTGMFH-WSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSND---DPTLADKEAILASLNIKA------------MTFDDTIGVLSQRGP---EFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGK------YGDLFVAALKSYGMLCIGLYRFRDT--SSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQ----FDPGLEYKPPA 1182
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0100622) HSP 1 Score: 518.079 bits (1333), Expect = 6.169e-163 Identity = 263/641 (41.03%), Postives = 398/641 (62.09%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDS-VSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTK 2305
+C RK++ F+ + GLL++++W+ RK + + + ++ +N + + +F + + +LIS Q GRI+ + + ++ S ++Y + ++E+C +N I DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q NV+K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN+ + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS+IPE+ E S+Y G Y K +RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++K PD+E++ L K +FT V++FQGT+M+ +DL RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P +SW NDY+RG ME+Y+ LS +F + + E CF L LLL+AIE+K + + +NP K I + GFFI S + A YC CH ++D T +K C CK LTV R K ++ +T+
Sbjct: 42 DCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEF--EGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVD--ASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLD---FNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAVFASWIPEITELAAQRSKYGGTYSKDPRKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAK-IQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCK------NLTVQPRSKFDDLGDITR 665 HSP 2 Score: 181.8 bits (460), Expect = 6.029e-46 Identity = 133/428 (31.07%), Postives = 215/428 (50.23%), Query Frame = 2
Query: 2291 VSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPS 3523
VSL ++ K+ +F ++ Y G F+ SP K +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C C IL+ K D D + DKE I+A+L +A + +I + + G E N S + G+ +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD V+ L Q G E+ PP+
Sbjct: 752 VSLPAGIADDQSKDFDF-EKTEMKYDSTGMFH-WSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSND---DPTLADKEAILASLNIKA------------MTFDDTIGVLSQRGP---EFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGK------YGDLFVAALKSYGMLCIGLYRFRD--TSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQ----FDPGLEYKPPA 1147
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0084684) HSP 1 Score: 517.309 bits (1331), Expect = 9.360e-163 Identity = 262/641 (40.87%), Postives = 400/641 (62.40%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDS-VSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTK 2305
+C RK++ F+ + GLL++++W+ RK + + + ++ +N + + +F + + +LIS Q GRI+ + + ++ S ++Y + ++E+C +N I DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q NV+K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN+ + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS IPE+ E + +G++Y G + G+RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++K PD+E++ L K +FT V++FQGT+M+ +DL RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P +SW NDY+RG ME+Y+ LS +F + + E CF L LLL+AIE+K + + +NP K I + GFFI S + A YC CH ++D T +K C CK LTV R K ++ +T+
Sbjct: 42 DCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEF--EGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVD--ASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLD---FNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAK-IQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCK------NLTVQPRSKFDDLGDITR 665 HSP 2 Score: 181.8 bits (460), Expect = 5.627e-46 Identity = 133/428 (31.07%), Postives = 215/428 (50.23%), Query Frame = 2
Query: 2291 VSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPS 3523
VSL ++ K+ +F ++ Y G F+ SP K +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C C IL+ K D D + DKE I+A+L +A + +I + + G E N S + G+ +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD V+ L Q G E+ PP+
Sbjct: 752 VSLPAGIADDQSKDFDF-EKTEMKYDSTGMFH-WSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSND---DPTLADKEAILASLNIKA------------MTFDDTIGVLSQRGP---EFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGK------YGDLFVAALKSYGMLCIGLYRFRD--TSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQ----FDPGLEYKPPA 1147
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Match: slo (gene:FBgn0003429 transcript:FBtr0100634) HSP 1 Score: 517.694 bits (1332), Expect = 1.301e-162 Identity = 262/641 (40.87%), Postives = 400/641 (62.40%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDS-VSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTK 2305
+C RK++ F+ + GLL++++W+ RK + + + ++ +N + + +F + + +LIS Q GRI+ + + ++ S ++Y + ++E+C +N I DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q NV+K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN+ + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS IPE+ E + +G++Y G + G+RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++K PD+E++ L K +FT V++FQGT+M+ +DL RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P +SW NDY+RG ME+Y+ LS +F + + E CF L LLL+AIE+K + + +NP K I + GFFI S + A YC CH ++D T +K C CK LTV R K ++ +T+
Sbjct: 42 DCLKVRKYWCFLLSSIFTFLAGLLVVLLWRAFAFVCCRKEPDLGPNDPKQKEQKASRNKQEF--EGTFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYFVD--ASSEEVERCQKWSNNITQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNVLKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPLD---FNNAHRLSYWTCVYFLIVTMSTVGYGDVYCETVLGRTFLVFFLLVGLAMFASSIPEIIELVGSGNKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGTIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDMQSWTNDYLRGTGMEMYTETLSPTFIGIPFAQATELCFSKLKLLLLAIEIKGAEEGADSKISINPRGAK-IQANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCK------NLTVQPRSKFDDLGDITR 665 HSP 2 Score: 181.415 bits (459), Expect = 7.067e-46 Identity = 133/428 (31.07%), Postives = 215/428 (50.23%), Query Frame = 2
Query: 2291 VSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPS 3523
VSL ++ K+ +F ++ Y G F+ SP K +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C C IL+ K D D + DKE I+A+L +A + +I + + G E N S + G+ +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD V+ L Q G E+ PP+
Sbjct: 774 VSLPAGIADDQSKDFDF-EKTEMKYDSTGMFH-WSPAKSLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGSVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCCILSAKVPSND---DPTLADKEAILASLNIKA------------MTFDDTIGVLSQRGP---EFDNLSATAGSPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTVESLSNRDRCRVGQISLYDGPLAQFGECGK------YGDLFVAALKSYGMLCIGLYRFRD--TSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQ----FDPGLEYKPPA 1169
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9]) HSP 1 Score: 647.506 bits (1669), Expect = 0.000e+0 Identity = 365/1064 (34.30%), Postives = 594/1064 (55.83%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFE---TACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTKCDVENDF-----------KEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETF-----QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---QDDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N +++ K++ + T + E+ + + + + + +IS Q L GR++ + V AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD++A+T G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK D++ T+ + K + +++E +D Y G F+ C +++ K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L N ++ SI + A + S N Q S + + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 37 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKNKEAQKVNNPITIQADGTTKTGNEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVYARTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSYIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPTVCELCYVKLKLLLIAIEYK-SEQRESSILINPGNHVKMQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRIEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRHDPLLIPGNEQIESMDANVKRYDSTGMFHWCPSKEIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVFILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLVRQASVTTGSNIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLGAPSQCTKRYVITNPPYEFEMVPTDLIFCLMQ 1073
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9]) HSP 1 Score: 647.121 bits (1668), Expect = 0.000e+0 Identity = 367/1068 (34.36%), Postives = 597/1068 (55.90%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFE---TACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVK----DSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTKCDVENDF-----------KEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETF-----QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---QDDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N +++ K++ + T + E+ + + + + + +IS Q L GR++ + V AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD++A+T G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K +S S+K+ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK D++ T+ + K + +++E +D Y G F+ C +++ K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L N ++ SI + A + S N Q S + + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 54 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKNKEAQKVNNPITIQADGTTKTGNEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVYARTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSYIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPTVCELCYVKLKLLLIAIEYKSEQRESRSRKR-ILINPGNHVKMQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRIEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRHDPLLIPGNEQIESMDANVKRYDSTGMFHWCPSKEIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVFILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLVRQASVTTGSNIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLGAPSQCTKRYVITNPPYEFEMVPTDLIFCLMQ 1094
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnma1a (potassium large conductance calcium-activated channel, subfamily M, alpha member 1a [Source:ZFIN;Acc:ZDB-GENE-070202-9]) HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0 Identity = 369/1080 (34.17%), Postives = 598/1080 (55.37%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFE---TACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTKCDVENDF-----------KEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL------------FFQAESK-------RRIMKNNSKIEMAKSISIKAAGGETQI--ERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---QDDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N +++ K++ + T + E+ + + + + + +IS Q L GR++ + V AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD++A+T G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK D++ T+ + K + +++E +D Y G F+ C +++ K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L QA S+ R +NS + + G I E K +F K S T + +I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 54 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKNKEAQKVNNPITIQADGTTKTGNEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVYARTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSYIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPTVCELCYVKLKLLLIAIEYK-SEQRESSILINPGNHVKMQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRIEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMRHDPLLIPGNEQIESMDANVKRYDSTGMFHWCPSKEIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVFILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLVRQASVTTGSNIPIITELAKPGKLLPLVSFSQEKNSGTNIQMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLGAPSQCTKRYVITNPPYEFEMVPTDLIFCLMQ 1118
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: CABZ01092907.1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1b [Source:NCBI gene;Acc:798583]) HSP 1 Score: 409.068 bits (1050), Expect = 2.071e-130 Identity = 200/467 (42.83%), Postives = 308/467 (65.95%), Query Frame = 2
Query: 605 RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPL-TNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVE 2002
+ + +IS Q L GR++ + ++ + V+Y I +E C N D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD+ AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E
Sbjct: 5 KDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSDPIESCEHFDKNFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSYIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPTVCE 462
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Match: kcnt1 (potassium channel, subfamily T, member 1 [Source:ZFIN;Acc:ZDB-GENE-091231-1]) HSP 1 Score: 139.813 bits (351), Expect = 6.587e-33 Identity = 99/329 (30.09%), Postives = 167/329 (50.76%), Query Frame = 2
Query: 1151 DSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGR--RHIIVCGHINYASVSTF----LTDFLHE---DRSSSDVDVIFINKKEPDIEMKSLLKI--YFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRS--FNEFSYKELVEFCF---KNLNLLLIAIEVKDSVSKKKMLLMNP 2089
++LYF IVT STVG+GD+ + ++ VV+ I +AL EL + G Y + + RH+++C VST L DFL+E + D V+ + E D++++ +L+I + +V Y QG+ + DL R +M +A+AC IL+ +N +D D I+RA + K++ CP+ +Q+L+ ENK ++ + D ++C E + +LA +CL P STL+T + +E +Q+P E W Y R E+Y +L S F E++ K F K + +I ++ +D+ K +L+NP
Sbjct: 215 NALYFCIVTFSTVGFGDVTPQIWPSQLLVVVMICVALVVLPLQFEELIYLWMERQKSGGNYSRHRAQTERHVVLC-------VSTLKIDLLMDFLNEFYAHPHTQDYYVVILCPCEVDVQVRRILQIPLWSQRVIYLQGSALKNQDLLRAKMDDAEACFILSSRNEADRMAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHV--------KFADHVVCEEEFKYAMLALNCLCPATSTLITLLVHTSRGQEGQQSP--EQWQRMYGRCSGNEVYHIRLGDSMFFREYNSKSFTYAAFHAHKKYGVCMIGVKREDN----KSILLNP 522
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898]) HSP 1 Score: 640.958 bits (1652), Expect = 0.000e+0 Identity = 373/1090 (34.22%), Postives = 605/1090 (55.50%), Query Frame = 2
Query: 380 MNIECPTQ--RKWYWFIAVDSLAYFGGLLLLVIWKI--------------------IINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG---------SPDDYELTVISRLKSEEEVSLTKCD----VENDFKEMEFIDEKCCS-YKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNN----QFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C Q R W+ F+A + +FGGL ++++W+ ++NA +++ +T + + ++E + + + + +IS Q L GR++ + TV AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K + ++++ NP N +I G++GFFI ++ A YC CH + D +K C CK SP + R ++ + D ND +++ +D Y G F+ C ++L K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + D++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + +ER++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ Q + P + + + Y D++ + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 21 MEVPCDNQGTRMWWAFLASSMVTFFGGLFIILVWRTFKYLWTVCCHCGGKNKDAQKVVNA---ASSQVTDGDYKQTDDKEETAAPGVVE-VGWMTSVKDWAGVMISAQTLTGRVL--VVTVFALSIGALMIY-FIDSSNPIETCQNFYKDFTLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRN--FENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPAVCELCFVKLKLLMIAIEFKSEKGESRIII-NPGNHVKIKEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGND--QIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGLLQANSQGFTPPGMERSSPDNSPVHGVARQASITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADL------GDGGCYGDLYCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1071
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898]) HSP 1 Score: 636.721 bits (1641), Expect = 0.000e+0 Identity = 371/1102 (33.67%), Postives = 607/1102 (55.08%), Query Frame = 2
Query: 380 MNIECPTQ--RKWYWFIAVDSLAYFGGLLLLVIWKI--------------------IINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK-----------------GSPDDYELTVISRLKSEEEVSLTKCD--------------VENDFKEMEFIDEKCCS-YKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---QDDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C Q R W+ F+A + +FGGL ++++W+ ++NA +++ +T + + ++E + + + + +IS Q L GR++ + TV AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K + + +++NP N +I G++GFFI ++ A YC CH + D +K C CK G +D + + +S K + + + ND +++ +D Y G F+ C ++L K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + D++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + +ER++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D++ + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 67 MEVPCDNQGTRMWWAFLASSMVTFFGGLFIILVWRTFKYLWTVCCHCGGKNKDAQKVVNA---ASSQVTDGDYKQTDDKEETAAPGVVE-VGWMTSVKDWAGVMISAQTLTGRVL--VVTVFALSIGALMIY-FIDSSNPIETCQNFYKDFTLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRN--FENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPAVCELCFVKLKLLMIAIEFKSEKGESR-IIINPGNHVKIKEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRRSQFHKSPLQNKKKGNGPFEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGND--QIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGLLQANSQGFTPPGMERSSPDNSPVHGVARQASITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLYCKALKTYNMLCFGIYRLRD-AHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1135
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898]) HSP 1 Score: 636.721 bits (1641), Expect = 0.000e+0 Identity = 371/1090 (34.04%), Postives = 602/1090 (55.23%), Query Frame = 2
Query: 380 MNIECPTQ--RKWYWFIAVDSLAYFGGLLLLVIWKI--------------------IINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG---------SPDDYELTVISRLKSEEEVSLTKCD----VENDFKEMEFIDEKCCS-YKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNN----QFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C Q R W+ F+A + +FGGL ++++W+ ++NA +++ +T + + ++E + + + + +IS Q L GR++ + TV AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FA ++PE+A + +Y G + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K + ++++ NP N +I G++GFFI ++ A YC CH + D +K C CK SP + R ++ + D ND +++ +D Y G F+ C ++L K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + D++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + +ER++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ Q + P + + + Y D++ + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 67 MEVPCDNQGTRMWWAFLASSMVTFFGGLFIILVWRTFKYLWTVCCHCGGKNKDAQKVVNA---ASSQVTDGDYKQTDDKEETAAPGVVE-VGWMTSVKDWAGVMISAQTLTGRVL--VVTVFALSIGALMIY-FIDSSNPIETCQNFYKDFTLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRN--FENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRKKYGGTFNATRGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPAVCELCFVKLKLLMIAIEFKSEKGESRIII-NPGNHVKIKEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGND--QIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGLLQANSQGFTPPGMERSSPDNSPVHGVARQASITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADL------GDGGCYGDLYCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1117
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898]) HSP 1 Score: 631.713 bits (1628), Expect = 0.000e+0 Identity = 371/1107 (33.51%), Postives = 604/1107 (54.56%), Query Frame = 2
Query: 380 MNIECPTQ--RKWYWFIAVDSLAYFGGLLLLVIWKI--------------------IINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKK-----------------------MLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG---------SPDDYELTVISRLKSEEEVSLTKCD----VENDFKEMEFIDEKCCS-YKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQ---DDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C Q R W+ F+A + +FGGL ++++W+ ++NA + ++ +T + + ++E + + + + +IS Q L GR++ + TV AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K + + +++NP N +I G++GFFI ++ A YC CH + D +K C CK SP + R ++ + D ND +++ +D Y G F+ C ++L K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + D++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + +ER++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D++ + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 4 MEVPCDNQGTRMWWAFLASSMVTFFGGLFIILVWRTFKYLWTVCCHCGGKNKDAQKVVNAASSQ---VTDGDYKQTDDKEETAAPGVVE-VGWMTSVKDWAGVMISAQTLTGRVL--VVTVFALSIGALMIY-FIDSSNPIETCQNFYKDFTLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRN--FENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPAVCELCFVKLKLLMIAIEFKSEKGESRGSKLEAVDILARFCVITFRVFFTRIIINPGNHVKIKEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGND--QIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGLLQANSQGFTPPGMERSSPDNSPVHGVARQASITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLYCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1078
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Match: KCNMA1 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:100491898]) HSP 1 Score: 629.787 bits (1623), Expect = 0.000e+0 Identity = 362/1009 (35.88%), Postives = 572/1009 (56.69%), Query Frame = 2
Query: 587 SFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG---------SPDDYELTVISRLKSEEEVSLTKCD----VENDFKEMEFIDEKCCS-YKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL------------FFQAESK-------RRIMKNNSKIE-MAKSISIKAAGGETQI-ERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQ---DDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
+ + + + +IS Q L GR++ + TV AL L +Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K + ++++ NP N +I G++GFFI ++ A YC CH + D +K C CK SP + R ++ + D ND +++ +D Y G F+ C ++L K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + D++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L QA S+ R +NS + +A+ SI I E K +F K S T +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D++ + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 19 GWMTSVKDWAGVMISAQTLTGRVL--VVTVFALSIGALMIY-FIDSSNPIETCQNFYKDFTLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWRN--FENSQDLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPAVCELCFVKLKLLMIAIEFKSEKGESRIII-NPGNHVKIKEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCACKRLEDEQPSALSPKKKQRNGGMRQSPNSSPNMMRHDPLLMTGND--QIDNMDSSSVKRYDSTGMFHWCPAKELDKVLLTRSEAAMTVLSGHVVVCIFGDMTSALIGVRNLVMPLRASNFHYHELKHIVFVGSLDYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASLNIKSMQFDDSIGLLQANSQGFTPPGMERSSPDNSPVHGVARQASITTGANIPIITELAKPGKLLPLISFSQEKNSGTHIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLYCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1010
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923]) HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0 Identity = 369/1078 (34.23%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNLESLIE-------DPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L+ + E + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGC+ D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 10 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1054
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923]) HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0 Identity = 369/1078 (34.23%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNLESLIE-------DPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L+ + E + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGC+ D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 10 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1054
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923]) HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0 Identity = 369/1078 (34.23%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNLESLIE-------DPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L+ + E + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGC+ D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 75 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1119
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923]) HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0 Identity = 369/1078 (34.23%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNLESLIE-------DPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L+ + E + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGC+ D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 10 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1054
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Match: Kcnma1 (potassium large conductance calcium-activated channel, subfamily M, alpha member 1 [Source:MGI Symbol;Acc:MGI:99923]) HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0 Identity = 369/1081 (34.14%), Postives = 613/1081 (56.71%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNLESLIE-------DPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG------------SPDDYELTVISRLKSEEEVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L+ + E + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGC+ SP + R L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 26 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLIYFEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1073
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q8AYS8|KCMA1_CHICK (Calcium-activated potassium channel subunit alpha-1 OS=Gallus gallus OX=9031 GN=KCNMA1 PE=1 SV=2) HSP 1 Score: 654.825 bits (1688), Expect = 0.000e+0 Identity = 367/1073 (34.20%), Postives = 608/1073 (56.66%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----------INAFHRKRLKISQSTNFETACR-SQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSE---------EEVSLTKCD--VENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQ---DDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + +++ T + AC+ + + E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL PG ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E F L LL+IAIE K S ++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D + ++++ +D Y G F+ C + + K +++ SE + L GH+++CIF D +++ IG+RN +MPLR+ +ELK +V + +++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + V+Q+ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 33 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQQLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYK-SEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1078
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q9BG98|KCMA1_RABIT (Calcium-activated potassium channel subunit alpha-1 OS=Oryctolagus cuniculus OX=9986 GN=KCNMA1 PE=2 SV=1) HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0 Identity = 369/1077 (34.26%), Postives = 612/1077 (56.82%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKIII-----------NAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSE---------EEVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + A +++ S T + E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 76 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKAKEAQKINNGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1120
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|O18866|KCMA1_PIG (Calcium-activated potassium channel subunit alpha-1 (Fragment) OS=Sus scrofa OX=9823 GN=KCNMA1 PE=2 SV=2) HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0 Identity = 371/1078 (34.42%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNLESLIE-------DPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSE---------EEVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N A ++ L+ + E + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 49 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGASQADGTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPSSSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1093
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|O18867|KCMA1_MACMU (Calcium-activated potassium channel subunit alpha-1 (Fragment) OS=Macaca mulatta OX=9544 GN=KCNMA1 PE=2 SV=1) HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0 Identity = 370/1078 (34.32%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSE---------EEVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 48 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1092
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Match: sp|Q28204|KCMA1_BOVIN (Calcium-activated potassium channel subunit alpha-1 OS=Bos taurus OX=9913 GN=KCNMA1 PE=1 SV=2) HSP 1 Score: 653.284 bits (1684), Expect = 0.000e+0 Identity = 370/1078 (34.32%), Postives = 615/1078 (57.05%), Query Frame = 2
Query: 380 MNIECPT--QRKWYWFIAVDSLAYFGGLLLLVIWKII-----INAFHRKRLKISQSTNFETACRSQKNL-------ESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSE---------EEVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRNNQFRINLTPVSQINKT-VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
M + C + QR W+ F+A + +FGGL ++++W+ + + + K +Q N + ++ L E++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L++ F S + AGF HL+ENSG+ + N+Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LL+IAIE K S +++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D+ T+ + K L + D ND +++ +D Y G F+ C+P+++ K +++ SE + L GH+++CIF D ++ IG+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L ++NVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ + T L R+ R + ++ ++ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 63 MEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKIN-NGSSQADGTLKPVDEKEETVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWEN--FQNNQALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLMIAIEYK-SANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPSSSPKLMRHDPLLIPGND--QIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRD---RCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1107
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A1B6HFZ4 (Uncharacterized protein OS=Homalodisca liturata OX=320908 GN=g.43282 PE=4 SV=1) HSP 1 Score: 674.085 bits (1738), Expect = 0.000e+0 Identity = 398/1127 (35.31%), Postives = 621/1127 (55.10%), Query Frame = 2
Query: 383 NIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG---SPDDYELTVISR---------LKSEEEVSLTKCDVEN-------------------------------------DFKEMEFIDEKC-CSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQA----ESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPSSDEKK*NS 3547
N EC +RKW+ F+ + GLL++++W++ RK + S + + ++ + + E +F + + +LIS Q GRI+ + + ++ S ++Y I ++EKC +N + DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q N++K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN Q + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS IPE+ E + S+Y G + G+RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++K PD+E++ L K +FT V++FQG++M+ +DL RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P ++W NDY++G E+Y+ L+ SF ++ + E CF L LLL+AIE+K + +NP K I + GFFI S + A YC CH ++D T +K C CK D + V +R + S ++V+ K +V D + +F EK Y G F+ SP + +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C CVIL+ K D D + DKE I+A+L +A ++ + +++S + A++ E N G +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD V+ L Q G E+ P S+ K+ NS
Sbjct: 18 NAECLKERKWWCFLLSSIFTFLAGLLIVLLWRVFAFLCCRKEPEFSPNDPKQKEQKAARQGKQEFEG-TFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYF--IDASSEEVEKCQSWSNNVTQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPME---FNNPQQLSYWTCVYFLIVTMSTVGYGDVYCQTVLGRTFLVFFLLVGLAIFASSIPEIIELVGTRSKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGSIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDTQAWQNDYLQGTGCEMYTETLAPSFTGMTFPQASELCFTKLKLLLLAIEIKGEDGNDSKISINPRGAK-ISANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKNCETGRDREDALVANRNLRNTPTAMVNSTKQVNKVKPNVNRTPNDIISPNSGYNNRPTQRGTPGNNVNNNGGLTVGIADDQAKDFDFEKTEMKYDSTGMFH-WSPARNLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGAVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCVILSAKVPSND---DPTLADKEAILASLNIKAMTFDDTIGVLSQDDSGVVSARN------------EHENLQTIGGGPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTSDSLSNRDRCRVGQISLYDGPLAQFGEAGK------YGDLFVSALKTYGMLCIGLYRFRD--TSSSCDASSKRYVITNPPDDFSLLPTDQVFVLMQ----FDPGLEYKPASNRGKEENS 1106
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A4W3HWC2 (Uncharacterized protein OS=Callorhinchus milii OX=7868 PE=4 SV=1) HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0 Identity = 383/1078 (35.53%), Postives = 602/1078 (55.84%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKI------IINAFHRKRLKISQSTN--FETACRSQKNLESLIEDPSFSVAF----RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETF-----QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
E QR W+ F+A + +FGGL ++++W+ + + K I + TN F A + KNLE E P V + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L + W+GLRF+R L LI+ E++Q N++K+ SI+++ LI+ F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLIL ++ C DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL PG ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D++ + + K L + D ND ++E +D Y G F+ C P+ + K +++ SE + L GH+++CIF D++++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ +V R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + S N Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 37 ESKEQRMWWAFLASSMVTFFGGLFIILLWRTFNYLWTVCCHCNPKNKDIQKFTNVVFSQADANSKNLEEKEELPVAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSDPIESCQDFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLSRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLISIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILTNKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSYIKIEE----DTWQKYYLEGVANEMYTEYLSGAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDKRESSILINPGNNVKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEHPSALSPKKKQRNGGMRNSPNGSPKLMRHDPLLIPGND--QIEEMDANVKKYDSTGMFHWCLPKDIEKVILTRSEAAMTVLSGHVVVCIFGDSKSALVGLRNLVMPLRASNFHYHELKHIVFVGALEYLKREWETLHNFPKVTIVAGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRASPDNSPVHGIVRQTSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HMCTPSPCIKRYVITNPPYEFEMVPTDLIFCLMQ 1077
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A1B6HKE7 (Uncharacterized protein OS=Homalodisca liturata OX=320908 GN=g.43307 PE=4 SV=1) HSP 1 Score: 667.537 bits (1721), Expect = 0.000e+0 Identity = 390/1104 (35.33%), Postives = 610/1104 (55.25%), Query Frame = 2
Query: 383 NIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKG---SPDDYELTVISR---------LKSEEEVSLTKCDVEN-------------------------------------DFKEMEFIDEKC-CSYKKNGNFYTCSPQKLMKNVISSEHQ--IENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQA----ESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGS--ETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---------QDDNDTEINLEQRN----NQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
N EC +RKW+ F+ + GLL++++W++ RK + S + + ++ + + E +F + + +LIS Q GRI+ + + ++ S ++Y I ++EKC +N + DLA N F++YF +RF +++KL F L ++ +D FTIP ++ LD+ WIGLRF+R L L+ V +++Q N++K+ +SIR+ QL++ F S+ + AG HLLENSG+ +NN Q + Y +YF+IVTMSTVGYGD++ +T+ G+ F+V F+L+ LA FAS IPE+ E + S+Y G + G+RHI+VCGHI Y SVS FL DFLHEDR DV+V+F+++K PD+E++ L K +FT V++FQG++M+ +DL RV++ ADACL+LA++ C DP+ EDA NIMR IS KNY VIIQL+Q+ NK YL NIP+W+ + GD +IC +E++LG +AQSCL PGFST++ N+F++RS K +P ++W NDY++G E+Y+ L+ SF ++ + E CF L LLL+AIE+K + +NP K I + GFFI S + A YC CH ++D T +K C CK D + V +R + S ++V+ K +V D + +F EK Y G F+ SP + +++ I +Q + L GH+++C+F+D + IG+RN +MPLR+ +ELK +VI+ D+I +EW + PKI V+ R ++++ C CVIL+ K D D + DKE I+A+L +A ++ + +++S + A++ E N G +GS VP+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMST Y+NQNA+ ++ LI GG+ +L+ + T +L R+ Q + P++Q + Y D+F+ + + + +GLYR R+ + + RYVITNPP +F + PTD + L Q
Sbjct: 18 NAECLKERKWWCFLLSSIFTFLAGLLIVLLWRVFAFLCCRKEPEFSPNDPKQKEQKAARQGKQEFEG-TFMTEAKDWAGELISGQTTTGRILVVLVFILSIASLIIYF--IDASSEEVEKCQSWSNNVTQQIDLAFNIFFMVYFFIRFIAASDKLWFMLEMYSFVDYFTIPPSFVSIYLDRTWIGLRFLRALRLMTVPDILQYLNILKTSSSIRLAQLVSIFISVWLTAAGIIHLLENSGDPME---FNNPQQLSYWTCVYFLIVTMSTVGYGDVYCQTVLGRTFLVFFLLVGLAIFASSIPEIIELVGTRSKYGGELKREHGKRHIVVCGHITYESVSHFLKDFLHEDREDVDVEVVFLHRKPPDLELEGLFKRHFTTVEFFQGSIMNPIDLQRVKVHEADACLVLANKYCQDPDAEDAANIMRVISIKNYSDDIRVIIQLMQYHNKAYLLNIPSWDWKQGDDVICLAELKLGFIAQSCLAPGFSTMMANLFAMRS---FKTSPDTQAWQNDYLQGTGCEMYTETLAPSFTGMTFPQASELCFTKLKLLLLAIEIKGEDGNDSKISINPRGAK-ISANTQGFFIAQSADEVKRAWFYCKACHEDIKDETLIKKCKCKNCETGRDREDALVANRNLRNTPTAMVNSTKQVNKVKPNVNRTPNDIISPNSGYNNRPTQRGTPGNNVNNNGGLTVGIADDQAKDFDFEKTEMKYDSTGMFH-WSPARNLEDCILDRNQAAMTVLNGHVVVCLFADPDSPLIGLRNLVMPLRASNFHYHELKHVVIVGAVDYIRREWKMLQNLPKISVLNGSPLSRADLRAVNVNLCDMCVILSAKVPSND---DPTLADKEAILASLNIKAMTFDDTIGVLSQDDSGVVSARN------------EHENLQTIGGGPIVLQRRGSVYGANVPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSTTYFNQNALTLIRSLITGGATPELELILAEGAGLRGGYSTSDSLSNRDRCRVGQISLYDGPLAQFGEAGK------YGDLFVSALKTYGMLCIGLYRFRD--TSSSCDASSKRYVITNPPDDFSLLPTDQAFVLLQ 1087
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A4W3HVA0 (Uncharacterized protein OS=Callorhinchus milii OX=7868 PE=4 SV=1) HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0 Identity = 383/1078 (35.53%), Postives = 602/1078 (55.84%), Query Frame = 2
Query: 389 ECPTQRKWYWFIAVDSLAYFGGLLLLVIWKI------IINAFHRKRLKISQSTN--FETACRSQKNLESLIEDPSFSVAF----RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEE---------EVSLTKCDV----ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETF-----QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
E QR W+ F+A + +FGGL ++++W+ + + K I + TN F A + KNLE E P V + + + +IS Q L GR++ + ++ + V+Y I +E C D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L + W+GLRF+R L LI+ E++Q N++K+ SI+++ LI+ F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y ++GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLIL ++ C DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL PG ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ +L+NP N +I G++GFFI ++ A YC CH + D +K CGCK D++ + + K L + D ND ++E +D Y G F+ C P+ + K +++ SE + L GH+++CIF D++++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ +V R ++I+ C CVIL+ N+ NI +D + DKE I+A+L N ++ SI + A + S N Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ T + RYVITNPP F + PTD+++CL Q
Sbjct: 38 ESKEQRMWWAFLASSMVTFFGGLFIILLWRTFNYLWTVCCHCNPKNKDIQKFTNVVFSQADANSKNLEEKEELPVAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSDPIESCQDFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLSRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLISIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILTNKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSYIKIEE----DTWQKYYLEGVANEMYTEYLSGAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDKRESSILINPGNNVKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRLEDEHPSALSPKKKQRNGGMRNSPNGSPKLMRHDPLLIPGND--QIEEMDANVKKYDSTGMFHWCLPKDIEKVILTRSEAAMTVLSGHVVVCIFGDSKSALVGLRNLVMPLRASNFHYHELKHIVFVGALEYLKREWETLHNFPKVTIVAGTPLSRADLRAVNINLCDMCVILSANQNNI----DDTSLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRASPDNSPVHGIVRQTSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HMCTPSPCIKRYVITNPPYEFEMVPTDLIFCLMQ 1078
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Match: A0A2I4B5Y2 (calcium-activated potassium channel subunit alpha-1-like isoform X12 OS=Austrofundulus limnaeus OX=52670 GN=LOC106517014 PE=4 SV=1) HSP 1 Score: 667.152 bits (1720), Expect = 0.000e+0 Identity = 374/1075 (34.79%), Postives = 614/1075 (57.12%), Query Frame = 2
Query: 383 NIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKC-YPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLK-----------SEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
++ C +QR W+ F+A + +FGGL ++++W+ + NA + RL++ + T + R+ K ++ + + + + + +IS Q L GR++ + ++ + V+Y I +E C Y + D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK +D + T+ + K S ++ + +++E +DE Y G F+ C + + K +++ SE + L GH+++CIF D +++ IG+RNF+MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L N ++ SI + A + ++R++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ T+ + RYVITNPP F + P+D+++CL Q
Sbjct: 28 DMPCDSQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNAKKKVRLEVHRITTGDGIKRADKE-DAAASEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSKPIESCQYFYKDYTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQALSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPVICELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALFYCKACHDDITDPKRIKKCGCKKFEEDQQSTLSPKKKQRNGGMRNSPNSSPKIMRHDPLLIPGSEQIESMDENVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNFVMPLRASNFHYHELKHIVFVGSLEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPENSPVHGLVRQTSVTTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLNTQSQCTKRYVITNPPYAFELVPSDLIFCLMQ 1066
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000050702.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000050702.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0 Identity = 372/1088 (34.19%), Postives = 602/1088 (55.33%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKIIINAF------HRKRLKISQSTNFETA------CRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTK--CDVEN---------DFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL------------FFQAESKR-------RIMKNNSKIE-MAKSISIKAAGGETQIERTNQSKSEGNET-FQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + + + K+ + + N A +K + + + + + + + +IS Q L GR++ + ++ + V+Y + +E C I D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD+ AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK + ++ T+ + K T C +N +ME +D Y G F+ C + + K +++ SE + L GH+++CIF D +++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L QA S+ R NS + + + S+ I +S N + K T +P+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 33 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNVKKKDVQRVINPIGAGDGVGKVGDKKEAAAAVSEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDS---SDPIESCQIFDEDITLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRFEEVHQSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASLNIKSMLFDDSIGLLQANSQGFTPPGMDRSSPENSPVHGLVRQTSVTTGANIPIITELAKSDRLLNAVCVRQDKSIGTNIPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSAPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1097
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000044980.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000044980.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 647.121 bits (1668), Expect = 0.000e+0 Identity = 377/1103 (34.18%), Postives = 604/1103 (54.76%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKIIINAF------HRKRLKISQSTNFETA------CRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLT-----------------KCDVEN---------DFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL------------FFQAESKR-------RIMKNNSKIE-MAKSISIKAAGGETQIERTNQSKSEGNET-FQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + + + K+ + + N A +K + + + + + + + +IS Q L GR++ + ++ + V+Y I +E C I D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD+ AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK S +Y +KS EEV + C +N +ME +D Y G F+ C + + K +++ SE + L GH+++CIF D +++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L QA S+ R NS + + + S+ I +S N + K T +P+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 33 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNVKKKDVQRVINPIGAGDGVGKVGDKKEAAAAVSEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYF---IDSSDPIESCQIFDEDITLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKSNYN----GYIKSIEEVHQSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASLNIKSMLFDDSIGLLQANSQGFTPPGMDRSSPENSPVHGLVRQTSVTTGANIPIITELAKSDRLLNAVCVRQDKSIGTNIPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSAPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1108
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000050440.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:48528:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000050440.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 644.81 bits (1662), Expect = 0.000e+0 Identity = 375/1103 (34.00%), Postives = 602/1103 (54.58%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKIIINAF------HRKRLKISQSTNFETA------CRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLT-----------------KCDVEN---------DFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL------------FFQAESKR-------RIMKNNSKIE-MAKSISIKAAGGETQIERTNQSKSEGNET-FQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + + + K+ + + N A +K + + + + + + + +IS Q L GR++ + ++ + V+Y + +E C I D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD+ AKT G++F+V FIL LA FA ++PE+A + +Y G Y GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK S +Y +KS EEV + C +N +ME +D Y G F+ C + + K +++ SE + L GH+++CIF D +++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L QA S+ R NS + + + S+ I +S N + K T +P+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 33 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNVKKKDVQRVINPIGAGDGVGKVGDKKEAAAAVSEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDS---SDPIESCQIFDEDITLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRKKYGGTYNSTRGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKSNYN----GYIKSIEEVHQSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASLNIKSMLFDDSIGLLQANSQGFTPPGMDRSSPENSPVHGLVRQTSVTTGANIPIITELAKSDRLLNAVCVRQDKSIGTNIPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLSAPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1108
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000051878.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000051878.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 632.098 bits (1629), Expect = 0.000e+0 Identity = 378/1134 (33.33%), Postives = 608/1134 (53.62%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKIIINAF------HRKRLKISQSTNFETA------CRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPD-----------------DYELTVISR-LKSEEEVSL----------------TKCDVENDFKEMEFIDEKCCSYKK---NGNFYT---CSPQKLMKN--VISSEHQIEN------------------------------------LRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + + + K+ + + N A +K + + + + + + + +IS Q L GR++ + ++ + V+Y + +E C I D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD+ AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK S DY +SR ++S E + +K + K +E + + S KK NG T CSP+ L + +I S Q+E L GH+++CIF D +++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L K + ++ + A S G ++R++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 33 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNVKKKDVQRVINPIGAGDGVGKVGDKKEAAAAVSEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDS---SDPIESCQIFDEDITLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKKACRRGTRLACCLDCGEADYGCLCMSRSVRSNAETAFPLSPTCLSDNSATLRSSKSNYNGYIKSIEEVHQSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASL----NIKSMLFDDSIGLLQANSQGFTPPG----MDRSSPENSPVHGLVRQTSVTTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLSAPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1135
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Match: ENSAMXT00000004500.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000822.1:37062:202682:-1 gene:ENSAMXG00000004398.2 transcript:ENSAMXT00000004500.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0 Identity = 381/1154 (33.02%), Postives = 609/1154 (52.77%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKIIINAF------HRKRLKISQSTNFETA------CRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPD-----------------DYELTVISR-LKSEEEVSL----------------TKCDVENDFKEMEFIDEKCCSYKK---NGNFYT---CSPQKLMKN--VISSEHQIEN------------------------------------LRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL------------FFQAESK-------RRIMKNNSKIE-MAKSISIKAAGGETQIERTNQSKSEGNET-FQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + + + K+ + + N A +K + + + + + + + +IS Q L GR++ + ++ + V+Y + +E C I D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQP+ Y + +Y ++VTMSTVGYGD+ AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK S DY +SR ++S E + +K + K +E + + S KK NG T CSP+ L + +I S Q+E L GH+++CIF D +++ +G+RN +MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L QA S+ R NS + + + S+ I +S N + K T +P+I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + PTD+++CL Q
Sbjct: 33 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNVKKKDVQRVINPIGAGDGVGKVGDKKEAAAAVSEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDS---SDPIESCQIFDEDITLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQPLSYWECVYLLMVTMSTVGYGDVCAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALYYCKACHDDITDPKRIKKCGCKRSKKACRRGTRLACCLDCGEADYGCLCMSRSVRSNAETAFPLSPTCLSDNSATLRSSKSNYNGYIKSIEEVHQSTLSPKKKQRNGGMRTSPNCSPKNLRHDPLLIPSHDQMETMDVNVKKYDSTGMFHWCPSKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSAVVGLRNLVMPLRASNFHYHELKPIVFVGSLEYLKREWETLHNFPKVSILPGSPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASLNIKSMLFDDSIGLLQANSQGFTPPGMDRSSPENSPVHGLVRQTSVTTGANIPIITELAKSDRLLNAVCVRQDKSIGTNIPMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLSAPSQCTKRYVITNPPYEFELVPTDLIFCLMQ 1163
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006399.1 (pep scaffold:Pmarinus_7.0:GL477091:41003:110164:-1 gene:ENSPMAG00000005728.1 transcript:ENSPMAT00000006399.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 358.221 bits (918), Expect = 3.358e-113 Identity = 170/385 (44.16%), Postives = 254/385 (65.97%), Query Frame = 2
Query: 1088 ENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSP 2242
ENSG+ + N Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FA ++PE+A + +Y G Y+ GR+H++VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L + +FTQV+++QG+V++ +DL RV++ ADAC+I+A++ C DP+ EDA NIMR IS KNY R VI Q+LQ+ NK +L NIP+W+ R GD IC SE++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LLLIAIE + S +L+NP + RI G++ FFI + ++ A YC CH + D +K C C+ P
Sbjct: 1 ENSGDPWRD--FQNQQNLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRRKYGGEYLPTRGRKHVVVCGHITLESVSNFLKDFLHKDRDDVNVEILFLHSISPNLELEALFRRHFTQVEFYQGSVLNPLDLARVKIEAADACMIIANKYCPDPDAEDASNIMRVISIKNYHPRIRVITQMLQYHNKAHLLNIPSWSWREGDAAICLSELKLGFIAQSCLAQGISTMLANLFSMRSYIKIEE----DTWQKFYLEGVSNEMYTEYLSSAFVGLSFPAVSELCFVKLRLLLIAIEYRSEHSYGSSILLNPGSHVRIQDGTLAFFIANDSKEVKRAFYYCKACHEDVTDPKKIKKCSCRQCP 379
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006404.1 (pep scaffold:Pmarinus_7.0:GL477091:42221:110230:-1 gene:ENSPMAG00000005728.1 transcript:ENSPMAT00000006404.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 356.295 bits (913), Expect = 2.691e-112 Identity = 169/383 (44.13%), Postives = 254/383 (66.32%), Query Frame = 2
Query: 1085 LENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCK 2233
+ENSG+ + N Q + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FA ++PE+A + +Y G Y+ GR+H++VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L + +FTQV+++QG+V++ +DL RV++ ADAC+I+A++ C DP+ EDA NIMR IS KNY R VI Q+LQ+ NK +L NIP+W+ R GD IC SE++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E CF L LLLIAIE + S +L+NP + RI G++ FFI + ++ A YC CH + D +K C C+
Sbjct: 22 VENSGDPWRD--FQNQQNLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRRKYGGEYLPTRGRKHVVVCGHITLESVSNFLKDFLHKDRDDVNVEILFLHSISPNLELEALFRRHFTQVEFYQGSVLNPLDLARVKIEAADACMIIANKYCPDPDAEDASNIMRVISIKNYHPRIRVITQMLQYHNKAHLLNIPSWSWREGDAAICLSELKLGFIAQSCLAQGISTMLANLFSMRSYIKIEE----DTWQKFYLEGVSNEMYTEYLSSAFVGLSFPAVSELCFVKLRLLLIAIEYRSEHSYGSSILLNPGSHVRIQDGTLAFFIANDSKEVKRAFYYCKACHEDVTDPKKIKKCSCR 398
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007042.1 (pep scaffold:Pmarinus_7.0:GL486096:7796:16492:-1 gene:ENSPMAG00000006371.1 transcript:ENSPMAT00000007042.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 226.868 bits (577), Expect = 4.483e-68 Identity = 105/215 (48.84%), Postives = 151/215 (70.23%), Query Frame = 2
Query: 1352 RHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSN---ESWLNDYMRGVTMEIYSAKLSRSFNEFSY 1987
RHI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ ADACLILA++ C DP+ EDA NIMR IS KNY R VI Q+ Q+ NK +L NIP+WN + GD IC +E++LG +AQSCL PG ST+L N+FS+RS + P ++W Y+ GV+ E+Y+ LS +F S+
Sbjct: 1 RHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIEAADACLILANKYCGDPDAEDASNIMRVISIKNYHPRIRVITQMQQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSYIKRVPLPHQIEEDTWQKYYLEGVSNEMYTEYLSNAFTGLSF 215
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: KCNT1 (potassium sodium-activated channel subfamily T member 1 [Source:HGNC Symbol;Acc:HGNC:18865]) HSP 1 Score: 96.2857 bits (238), Expect = 5.040e-21 Identity = 60/207 (28.99%), Postives = 106/207 (51.21%), Query Frame = 2
Query: 1130 SQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGR--RHIIVC-GHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKI--YFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQ 1735
++ I SLYF IVT STVG+GD+ K ++ VV+ I +AL EL + G Y + + +H+++C + + FL +F R D + + + D +++ +L + + +V Y QG+ + DL RV++ +A+AC IL +N D D I+RA + K++ CP+ +Q+L+ ENK +++
Sbjct: 219 AENISLFRSLYFCIVTFSTVGFGDVLPKIWPSQLLVVIMICVALMVLPVQFEELVYLYMERQKSGGNYSRYRAQTEKHVVLCVSSLKLDLLMDFLNEFYAHPRLQ-DFYTVILCPTDMDSQVRRMLHVPLWAQRVIYLQGSALKDQDLVRVKIDDAEACFILTSRNEVDRTAADHQTILRAWAVKDFAPSCPLYVQILKPENKFHVK 424
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Match: KCNA3 (potassium voltage-gated channel subfamily A member 3 [Source:HGNC Symbol;Acc:HGNC:6221]) HSP 1 Score: 60.8474 bits (146), Expect = 1.351e-9 Identity = 66/259 (25.48%), Postives = 110/259 (42.47%), Query Frame = 2
Query: 752 LTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLD--------QEWIGLRFIRVLSLIRVFEVIQCFNVVKS--------GTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIK------IAGRRHIIVCGHINYA----SVSTFLTDFLH-EDRSSSDVD 1447
T+P F + C F L+RFF K F+ +ID I L + Q+ + L +RV+ L+RVF + + K S+R + L+ +F L +G F +S + + + NS+ D+ ++ +VTM+TVGYGD+ T+ GKI L + + T A +P + S + FY + A H+ C H + S S ++++ +D S+DVD
Sbjct: 210 FTDPFFIVETICIIWFSFELLVRFFACPSKPGFFKSIMNIIDIVAILPYFITLGTEMAKHQGSGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFF---LFIGVILF----SSAVYFAEADHQNSEFRSIPDAFWWAVVTMTTVGYGDMRPVTVGGKIVGSLCAIAGVLTIALPVPVIV------SNFNYFYHRETDNEEHANYLHVSSCSHQEESRKSRSPSMSRSEYMEMDDGLSNDVD 455
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Match: EDO32743 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU69]) HSP 1 Score: 123.635 bits (309), Expect = 1.583e-28 Identity = 105/373 (28.15%), Postives = 175/373 (46.92%), Query Frame = 2
Query: 1001 KSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIK-IAGR-RHIIVCG-HINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLK--IYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRS--FNEFSYKELVEFCF---KNLNLLLIAIEVKDSVSKKKMLLMNP 2089
+S S +V+ LIT IL H LE + S +L+F+IVT STVGYGD + FV++ I +AL + LA LF+ + G Y +AG RH+++C + ++ FL +F + D DV V+ + + D ++ LL+ ++ +V Y +G+ + DL R + NA C ILAD+ +D D I+R + +++ P+ +Q+L+ ENK ++ + ++C EI+ +LA +C+ PG STL+T + E+ + +E W Y + EIY +L S F ++YK F K + LI ++ K L++NP
Sbjct: 215 QSALSQKVLILITTVVCILFTSVCGIHHLERA-----------SLQWSMMKTLWFVIVTFSTVGYGDTVPTHWTTQTFVMIMIGIALVLLPIELERLAFLLFSRQKEGGAYNHLLAGTDRHVVLCATTLRTGTLIDFLNEF-YADSRLHDVHVVLLCPSDLDSTLRILLQVPVWAQRVTYLKGSALIDEDLVRAR-RNAAGCFILADRYAADREAADQHTILRTWAIQDFAPATPLFVQILKPENKFHVS--------FAEHVVCEDEIKHALLAVNCVCPGISTLVTLLLHTLHEQYVR---GSEKWHEIYGKCAGNEIYDIRLGDSMIFRPYAYKSFTHAAFHAHKKYGVTLIGVKRH---GKGGRLMLNP 560
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Match: EDO28098 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7F3]) HSP 1 Score: 67.781 bits (164), Expect = 8.710e-13 Identity = 32/111 (28.83%), Postives = 61/111 (54.95%), Query Frame = 2
Query: 2456 HIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANL 2788
HI++C+F+ +G+R +MPLR+ + ++LK++V + DKD+++ EW EIC F ++ ++ R ++ + V L+ D D + DK+ I+A+L
Sbjct: 72 HILVCVFNKKEDPLVGLRKMVMPLRASNLHRSDLKKIVFLGDKDYMKLEWEEICNFHEVYILPGSPFCRADLRAVNANLADMVVFLSGTA--VDQAGDPRLADKQSILASL 180
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Match: SHAK5 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAS8]) HSP 1 Score: 61.2326 bits (147), Expect = 1.158e-9 Identity = 50/160 (31.25%), Postives = 80/160 (50.00%), Query Frame = 2
Query: 797 FLIYFLLRFFTSTEKLAFWLGFHTVIDC-----FTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSG---------TSIRVIQLITYFASILIVGAGFFHLLENSGEI-TSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKI 1231
F + +L+RF +S +K F F VID F I IL ++ + + L F+R+L L+RVF + + + SG S+R + L+ +F L +G F + E T + S P D+ ++ +VTM+TVGYGD+ KT+ GK+
Sbjct: 199 FTLEYLVRFLSSPKKCPFVRSFLNVIDILAIVPFYIDLILNSVQSNDGTLSLAFLRILRLVRVFRIFK-LSRHSSGLQILGLTLRKSLRELGLLIFF---LFIGVVIFSSMAYYAEAGTKDTHLGRSIP----DTFWWALVTMTTVGYGDLVPKTLWGKL 350
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Match: SHAKR1 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3Y6]) HSP 1 Score: 57.7658 bits (138), Expect = 1.297e-8 Identity = 85/309 (27.51%), Postives = 140/309 (45.31%), Query Frame = 2
Query: 398 TQRKWYWF----IAVDSLAYF---GGLLLLVI---WKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQ--ILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIG-YGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIP 1285
+ +K Y+F +A DS+ YF G LL + K +N + L+ S F A S +L S + D + V +L + F ++VA + LLS V++ E LP E ++ ++ ++ C F + LLRF T K F + F +ID I I+ L ++ L F+R+L L+RVF + F + + ++V+ L T AS+ + F LL +SG +Y S ++ +F +VTM+TVGYGD + GK+ + + + T A +P
Sbjct: 39 SMKKEYFFDRNRVAFDSILYFYQSNGKLLRPVDLDMKTFVNEVYFFELEDLVSHQFIEAYGSDTSLVSKMPDNAIQRKIWE-VLELPHSSF-TAQVVAVWSVGVILLSIVVFCIE--TLPGLRETRTEKSSALYSIEILCVAWFTLELLLRFLTCPNKPHFIISFLNIIDVLAILPYYIMLGLGDTKDTSTLAFLRILRLVRVFRI---FKLSRHYRGLKVLGL-TLKASLRELLLLTFFLLMGVIVFSSGVYYAESTHFSSIPEAFWFSVVTMTTVGYGDKAPLSTWGKLVGSMCAIAGVLTLALPVP 339
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Match: EDO47871 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RL57]) HSP 1 Score: 56.225 bits (134), Expect = 5.114e-8 Identity = 66/297 (22.22%), Postives = 125/297 (42.09%), Query Frame = 2
Query: 488 NAFHRKRLKISQSTNFETACRSQKNLESLIE--DPSFSVAFRSYVNQLISTQF---------LIGRIVASIATVAALLSTV-LYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQI---LTALVLDQEWIGLRFI---RVLSLIRVFEVIQCFNVVKSGTSIRVIQL--------ITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGK-------IFVVLFILLALATFAS 1276
N FH++ + + A + N++ + D V RS + +L + + +IV +++ +LSTV L L I L +KL+ N + + C F + +L+R ++ + F ID I L +L D + + RF+ R+L ++R+ +++ F + + ++ + + ++LI+G F L E + S P ++ ++ I+TM+TVGYGD++ T GK I ++FI L + T S
Sbjct: 120 NHFHQENEAVENLMKIKEAFKKDLNMKKEQDEVDLLLEVESRSKLARLKRKMWDLFENPHSSFMAKIVYLLSSFFVMLSTVALCLNTIDSLRIKLDNGETADNAVLAGIEAGCIAWFTVEYLIRLLSAPRIVPFLKSIMNTIDLLAILPYYFELLSLAFDNQ-LDYRFVEMRRILQILRILRIVRIFKMARHSVGLQTLAYTFKQSTAELGLLGTLLIMGMTLFSSLVYFAEEAEPNSMFKSIP----EAFWWSIITMTTVGYGDVYPVTPLGKLVGSLCCISGIIFIALPIPTIVS 411
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: ENSORLT00000011055.2 (potassium calcium-activated channel subfamily M alpha 1 [Source:NCBI gene;Acc:101155390]) HSP 1 Score: 659.062 bits (1699), Expect = 0.000e+0 Identity = 367/1054 (34.82%), Postives = 611/1054 (57.97%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKIIINAF------HRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTKCD------VEND------FKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETF-QVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLD---QDDNDTEINLEQRNNQFRINLTPVSQI----NKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + + K+ ++ + T E R K +S + + + + + +IS Q L GR++ + ++ + V+Y + P++ K + +N D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C+DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++LG +AQSCL G ST+L N+FS+RS + ++ ++W Y+ GV+ E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK +D + +S K + + + +D +++E +DE Y G F+ C + + K +++ SE + L GH+++CIF D +++ IG+RNF+MPLR+ +ELK +V + +++++EW + FPK+ ++ R ++I+ C CVIL+ N+ NI +DA + DKE I+A+L K + ++ ++ A S G ++R++ S + Q S + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ ++N + + V+Q+ + + Y D+F + + + + G+YR R+ + + RYVITNPP F + P+D+++CL Q
Sbjct: 36 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCKAKKKEVHRITTGEHIKRVDKG-DSAASEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDS-SDPIEFCKHF-YSNYTLQIDMAFNIFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQALSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEE----DTWQKYYLEGVSNEMYTEYLSSAFVGLSFPVICELCYVKLKLLLIAIEYK-SDQRESSTLINPGNHVKMQEGTLGFFIASDAKEVKRALFYCKACHDDISDPKRIKKCGCKKFEEDRH-SALSPKKKQRNGGMRNSPNSSPKIMRHDPLFIPGSEQIESMDENVKKYDSTGMFHWCPSRDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNFVMPLRASNFHYHELKHIVFVGSLEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----DDASLQDKECILASL----NIKSMLFDDSIRVLQANSQGFTPPG----MDRSSPENSPVHGLVRQTSVTTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEGLLAEENALKGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLNAQSQCTKRYVITNPPYAFELVPSDLIFCLMQ 1065
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692]) HSP 1 Score: 651.358 bits (1679), Expect = 0.000e+0 Identity = 375/1074 (34.92%), Postives = 602/1074 (56.05%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAF----RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLK------------SEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNE-TFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N ++ K++ N + A R K + E P+ V + + + +IS Q L GR++ + V AL L +Y I +E C + D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS E ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK D++ T+ + K S + +S + +++E +D Y G F+ C + + K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI DA + DKE I+A+L N ++ SI + A + ++R++ S + Q S + + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + P+D+++CL Q
Sbjct: 58 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKKKEVQKVNNFANNQAADRPPKGPDEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQSFYDDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSFIEIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPHICELCYVKLKLLLIAIEYK-SEQRESSILINPGNHVKMQEGTLGFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGCKRLEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----EDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDSSPVHGFVRQASVTTGSNIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLGAPSQCTKRYVITNPPYEFELVPSDLIFCLMQ 1096
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692]) HSP 1 Score: 645.195 bits (1663), Expect = 0.000e+0 Identity = 381/1094 (34.83%), Postives = 607/1094 (55.48%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAF----RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVK----DSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLK------------SEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANL---FFQAESKRRIMKNNSK------IEMAKSISIKAAGGETQIERTNQSK--------SEGNETFQVS----KGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N ++ K++ N + A R K + E P+ V + + + +IS Q L GR++ + V AL L +Y I +E C + D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS E ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K +S S+K+ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK D++ T+ + K S + +S + +++E +D Y G F+ C + + K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI DA + DKE I+A+L Q + +++ NS+ ++ + S G Q T S G VS K S T + +I++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + P+D+++CL Q
Sbjct: 58 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKKKEVQKVNNFANNQAADRPPKGPDEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQSFYDDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSFIEIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPHICELCYVKLKLLLIAIEYKSEQRESRSRKR-ILINPGNHVKMQEGTLGFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGCKRLEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----EDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDSSPVHGFVRQASVTTGSNIPIITELAKPGKLMPLVSHSQEKKSGTSIQMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLGAPSQCTKRYVITNPPYEFELVPSDLIFCLMQ 1128
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692]) HSP 1 Score: 642.114 bits (1655), Expect = 0.000e+0 Identity = 377/1107 (34.06%), Postives = 604/1107 (54.56%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAF----RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVK----DSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSL---TKCDVEND--------------------------------------FKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNE-TFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N ++ K++ N + A R K + E P+ V + + + +IS Q L GR++ + V AL L +Y I +E C + D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FAS++PE+ E + +Y G Y + GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C DP+ EDA NIMR IS KNY + +I Q+LQ+ NK +L NIP+WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS E ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K +S S+K+ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK + T S++E + C++ D +++E +D Y G F+ C + + K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI DA + DKE I+A+L N ++ SI + A + ++R++ S + Q S + + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + P+D+++CL Q
Sbjct: 58 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKKKEVQKVNNFANNQAADRPPKGPDEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQSFYDDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVNGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCGDPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSFIEIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPHICELCYVKLKLLLIAIEYKSEQRESRSRKR-ILINPGNHVKMQEGTLGFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGCKRPAEG--ATTPGNTGSQKEAGVRFKADCNIVEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----EDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDSSPVHGFVRQASVTTGSNIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRD-AHLGAPSQCTKRYVITNPPYEFELVPSDLIFCLMQ 1127
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Match: kcnma1a (calcium-activated potassium channel subunit alpha-1 [Source:NCBI gene;Acc:101174692]) HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0 Identity = 359/1074 (33.43%), Postives = 580/1074 (54.00%), Query Frame = 2
Query: 401 QRKWYWFIAVDSLAYFGGLLLLVIWKII---------INAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAF----RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLK------------SEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVIS-SEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILN-NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGE----TQIERTNQSKSEGNE-TFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINL-TPVSQINKT--------------VNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
QR W+ F+A + +FGGL ++++W+ + N ++ K++ N + A R K + E P+ V + + + +IS Q L GR++ + V AL L +Y I +E C + D+A N FL+YF LRF + +KL FWL ++V+D FT+P + ++ L++ W+GLRF+R L LI+ E++Q N++K+ SI+++ L + F S + AGF HL+ENSG+ + NSQ + Y + +Y ++VTMSTVGYGD++AKT G++F+V FIL LA FA ++PE+A + +Y G Y GR+HI+VCGHI SVS FL DFLH+DR +V+++F++ P++E+++L K +FTQV+++QG+V++ DL RV++ +ADACLILA++ C DP+ EDA NIMR + WN + GD IC +E++ G +AQSCL G ST+L N+FS+RS E ++ ++W Y+ GV E+Y+ LS +F S+ + E C+ L LLLIAIE K S ++ +L+NP N ++ G++GFFI ++ A YC CH + D +K CGCK D++ T+ + K S + +S + +++E +D Y G F+ C + + K +++ SE + L GH+++CIF D ++ +G+RN +MPLR+ +ELK +V + D++ +EW + FPK+ ++ R ++I+ C CVIL+ N+ NI DA + DKE I+A+L N ++ SI + A + ++R++ S + Q S + + +PII++L +SNVQ+LD +D+ + YL +PFA G AF S+LDSLMS Y+N N + ++ L+ GG+ +L+ L N R TP + N+ + + Y D+F + + + + G+YR R+ + RYVITNPP F + P+D+++CL Q
Sbjct: 58 QRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCNIKKKEVQKVNNFANNQAADRPPKGPDEKEEAPASEVGWMTSVKDWAGVMISAQTLTGRVL--VVLVFALSIGALGIY-FIDSSDPIESCQSFYDDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWEN--FQNSQSLSYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFARYVPEIAALILNRKKYGGSYNSTRGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCGDPDAEDASNIMRYTLYH-------------------------IWNWKEGDDAICLAELKAGFIAQSCLAQGLSTMLANLFSMRSFIEIEE----DTWQKYYLEGVANEMYTEYLSSAFVGLSFPHICELCYVKLKLLLIAIEYK-SEQRESSILINPGNHVKMQEGTLGFFIASDAKEVKRAFYYCKACHDDITDPKRIKKCGCKRLEDEHPSTLSPKKKQRNGGMRNSPNCSPKMMSRHDPLLIPGNEQIENMDMNIKRYDSTGMFHWCPSKDIEKVILTRSEASMTVLSGHVVVCIFGDVTSALVGLRNLVMPLRASNFHYHELKPIVFVGSLDYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNI----EDASLQDKECILASL------------NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDSSPVHGFVRQASVTTGSNIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELE--------GLLAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDA-HLGAPSQCTKRYVITNPPYEFELVPSDLIFCLMQ 1071
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000044594.1 (SMESG000044594.1) HSP 1 Score: 2151.71 bits (5574), Expect = 0.000e+0 Identity = 1047/1053 (99.43%), Postives = 1049/1053 (99.62%), Query Frame = 2
Query: 380 MNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKGKEFSPPSSDEKK 3538
MNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFR YVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDL RVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSP DYELTVISRLKSEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDD+DTEINLEQRNNQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVET+DRYVITNPPPNFPIEPTDMVYCLQQKCLPKPK KEFSPPSSDEKK
Sbjct: 1 MNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRPYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLHRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPGDYELTVISRLKSEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDSDTEINLEQRNNQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNLLRKETRVETIDRYVITNPPPNFPIEPTDMVYCLQQKCLPKPKRKEFSPPSSDEKK 1053
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000014623.1 (SMESG000014623.1) HSP 1 Score: 638.647 bits (1646), Expect = 0.000e+0 Identity = 371/1048 (35.40%), Postives = 612/1048 (58.40%), Query Frame = 2
Query: 398 TQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKIS----QSTNFETACRSQKNLESLIEDPSFSVAF-RSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKC--YPLTNPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQ-NPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKS-EEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIK-VVFHQISIRRIAHLISIDTCSRCVILN---NKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVP---IISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINLTPV--SQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYR----KRNLLRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQ 3475
+++KWY F+ + + F GL LL++WK+I + + K+S +++ S N I + + ++ R + ++ IS Q G+I+ IA V ++ Y+YE P+++E+C Y + DL C F Y+++RF + K+ FW G ++++D TIP + LD W+GLRF+R + L++V +++Q V+KS T R+ QL T F + IVG+G H+LEN+G+ + N+ + +GDS+YF IVTMSTVGYGDI + GKI+V +FIL++ A FASFIPE+AE LF+GS+Y G Y+ +HI+VCG+ ++S+ +F+ DF +EDR S ++V+FIN++EPD E+++LLK+ F + K+ +GTVMDQVDL R + ADAC ILA+ NC +P DEDA NIMR IS KN+ V+IQLLQ+ENK+YL NIPTWNK GD ++CYSE+EL ILA+SCL+PG+ST ++N+FS+RSE +CK+ S SW+NDY+ G+ +E+Y++ LS S ++ +LV F ++ LNLLLIA+EVK + K + +NP + +VG+FIC+S++++ +YC+ CH + T L+V D ++ I+++ S E++ T + + +D+ +K +F C + +++I + + HIILCIF+DN + SIG++NF++PLRS + +L+ ++I ++++F+ +EW + FP + + F+ I+ + ++ ++ ++ C CVI++ +K N A+L D ++VDKEII+ L ++ K+++ K K I G ++P +I+KL +E+NVQ+LD +++ N P +L + FA G AFTT MLD L+S AY+N + ++ LI G + + +++ N+ N + +I + + + +N Y+++F + + LGLY+ +N+L + + ++TNP F + D V LQ
Sbjct: 5 SEKKWYVFLLTNVIIIFSGLFLLLLWKLIKFIYGKISKKLSPINASQGSYKAGEFSGGNNSGRINEENQNIQIIRVWTSKFISGQNTTGKIIVFIACVCTFITVGDYIYEATYFPMQIERCGIYYEGAFLLAIDLFCYSFFTCYYIIRFLAAENKIEFWFGVYSLVDLLTIPSFFVEIYLDHLWMGLRFLRAIWLMQVPDLLQYLGVLKSATKFRLGQLSTMFICMWIVGSGMTHMLENTGDTWLSAEVNDKNFLTFGDSMYFTIVTMSTVGYGDISPHSFLGKIWVAIFILVSFAIFASFIPEIAEILFSGSKYTGSYVPKEEYKHIVVCGNFEFSSIQSFMADFFNEDRPISKINVVFINQEEPDFELRNLLKMNFMRSKFIKGTVMDQVDLKRASIDEADACFILANHNCKNPKDEDAANIMRVISLKNFSSSVRVLIQLLQYENKSYLTNIPTWNKDKGDTVVCYSEMELRILAKSCLIPGYSTFISNLFSIRSEAQCKEFRKSGNSWINDYINGMGLELYTSNLSLSLIGMTFYDLVLFSYEYLNLLLIAVEVK--MRNGKTVFINPASQSYTTKETVGYFICNSFKESQRVALYCNVCHGA-NEGTPLRV-------DKFKCVCINKVASLNEKIRQTSFRLSRQQFQYAILDKA----EKEIDFLKCDYKDFKESLIKNPLEPIKFSNHIILCIFADNDSPSIGLKNFIIPLRSKKLDTADLRPIIIASNEEFLHREWRSLACFPNLHPISFNNINKKSLS-VLQVENCHVCVIMSARVSKRNAANL--DFHLVDKEIILGTLNVKSIFKKKLQK--------KFI-----------------------------GKSGEIPNRFLITKLLVENNVQFLDQEDEDEPNCPVFLSEIFACGSAFTTKMLDCLLSIAYFNPDVNKVITHLI-AGEIHCFENINDEESTNMANNNVKCQIRIFNLFDDKFQSCFANTNRCLYKNLFTYAFKHYHLLLLGLYKVVETTQNILSSSKK-----KVILTNPDGEFSVSCYDSVLGLQ 992
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000050831.1 (SMESG000050831.1) HSP 1 Score: 635.565 bits (1638), Expect = 0.000e+0 Identity = 383/1092 (35.07%), Postives = 609/1092 (55.77%), Query Frame = 2
Query: 407 KWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKR-LKISQSTNFETACRSQKN-LESLIEDPSFSVA-------FRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKMLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPD---------DYELTVI--SRLKSEEEVSLTKCDV-------------------ENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVI---SSEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAES---KRRIMKNNSKI--EMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQD------------DNDTEINLEQ-RNNQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNL-------LRKETRVETVDRYVITNPPPNFPIEPTDMVYCLQQ 3478
KWY F+A G+L++ +++I N R ++ N S+ + +++ +D V + + ++IS Q L G+ + S+ + ++ S +YLYE+ E C + DLA N +FL+YF LRF S +KL FWL ++ +D +TIP L L + WIGLRF+R + L+ + +V+ NV+KS +SIR+ L T F S++I +GF HL+E+SG +N Q + D YFIIVT+STVGYGDI +T GK+ ++ FIL LA FA+ IPE+ E + + ++Y + K ++HII+CGHI Y SVS FL DFLH+DR DV ++ +N P++E+K++LK YF V+++QG+ M+ DL R+Q+ NADACL+LA++ DP+ EDA NIMR IS KN+C R VI+QL+Q+ NK YL NIP+W+ + GD +C +E++LG +AQSCL PGFSTLL N+F++RS K P +WLN Y+ G ME+YS S +F+ ++ E V FK L LLLIAIE K +V+++ + +NP N +I PG GFFI S ++ A +C +CH R +K C C +E T S+L+S++ +K + + F+ + F ++ + G F+ C P++ +++V+ S + ++ HI++C+F+++ ++ IG+ NF+MPLR+ +EL +V I + +++++EW + FP + ++ R ++++ CVIL++K+N + +DA++VDKE I+ +L QA S M+N ++ +++KS+S + G E + SKS + + +I++LAI+SNVQ+LD +++ ++ +PFA G AF S+LDSLMSTAY+N+NA+ ++ LI GG+ DL+Q DT N ++ R ++ P+++I + N Y +F+E + + + +GLYR NL + RYVITNPP I +DMVYCLQQ
Sbjct: 19 KWYIFLATSVACQLCGILIIGFYRVIQNLSRWFRNPNVNSKLNLTDEGLSRPDFIDTKFDDDQVHVEISNCFSDLKDWSGEIISGQNLTGKTLVSLVCILSIGSIAIYLYELSVYDGLTEVCVKWNRSLTQQLDLAFNIIFLVYFFLRFLASNDKLCFWLELYSFVDLYTIPPSFVILYLGRYWIGLRFVRAMRLMTLSDVLGFLNVIKSSSSIRLCHLFTTFVSLVITASGFIHLVESSG---GPVIADNGQNRNFLDFTYFIIVTISTVGYGDIVCRTPTGKLMMMFFILGGLALFANSIPEMIEIIGSRNRYRNPFRKQKDKQHIILCGHITYDSVSNFLADFLHKDREDVDVKIVILNNFPPELELKNVLKRYFIHVEFYQGSGMNSNDLSRIQLQNADACLVLANKYSPDPDAEDAANIMRVISIKNFCPRIKVIVQLMQYHNKAYLLNIPSWDWKRGDDAVCLAELKLGFIAQSCLAPGFSTLLANLFTMRSFKNDANLP---NWLNTYLEGAGMEMYSEYFSPAFHGCTFAEAVAMSFKYLKLLLIAIETKSTVNEENSIAINPKNDIKIEPGMQGFFIAQSADESKRAFFFCRKCHLN-RHYKYIKQCSCAAHKQFQNTLHKLRKFETTKSRHSKLESKKGDESSKVKLRRNRNIRPNERKRPHNIRNQKSFRGLRFNEQISRRFDTTGMFHWC-PERPIEDVLLNDSDQLKMCVFSNHIVVCVFAESNSALIGLCNFVMPLRASNFHYSELSYIVFIGNLEYLKREWKSLANFPCLSILSGSSLSRTNLRAVNVNMSKMCVILSSKSN--NSMDDAFLVDKEAILCSLNVQAMSFDIDSFKMENFPELLPQISKSVSYSSFGMEKDMTDDKISKSGAD------------IKMITELAIDSNVQFLDQDDEDSPETELFMTQPFACGRAFAVSVLDSLMSTAYFNENALTLIRTLITGGATPDLEQVLAEGCGIQGAYLTPDTLGNRDRCRVTIIQVTQNPLAEIFISKNGGCN--YGHLFLEALERFGILCIGLYRLINLDNLVNDNAGMMLNTNSKKRYVITNPPDFLQIHYSDMVYCLQQ 1086
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000076792.1 (SMESG000076792.1) HSP 1 Score: 527.324 bits (1357), Expect = 1.991e-167 Identity = 344/1026 (33.53%), Postives = 543/1026 (52.92%), Query Frame = 2
Query: 560 NLESLIEDPSFSVAFRSYVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLP-LKLEKCYPLTNPIFCS-DLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKK--MLLMNPLNCKRIIPGSVGFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSP--DDYELTVIS-----------------------------RLKSEEEVSLTKCDVEND-FKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQIEN--LRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDN------------DTEINLEQ-RNNQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRK--RNLLRKETRVET-VDRYVITNPPPNFPIEPTDMVYCLQ 3475
NL+ +IE + + ++IS Q GR++ SI + +L S ++Y EI L + E C + DLA N VFLI+F LR+ +T+K FW+ ++++D T+P VL++ W+GLRF R L LI +V+ N++KS + IR+ L+T F S+++ AGF HL+E +G+I + N Q Y D YF+I TMSTVGYGDI KT GK+ ++ FIL LA FA+ IPE+ E + ++Y G + K G++HII+CGHI + R++ D P E S+ K V + RVQ+ ADACLIL D+ DP+ EDA NIMR IS KNYC VI+QL+Q+ NK YL NIP+W+ R GD ++C +E++LG +AQSCL PGFSTLL N+F +RS K+ P W+N Y+ G ++E+Y+ LS SF+ ++ E V CF LNLLLIA+EVK + + + +NP I PG+ GFFI +S DA A YC CH + + ++K C C+ S D ++S R + + + + ND ++F ++ + G F+ CS ++ +++++ + + HI++CIF+D + IG+R+F+MPLRS NEL++++ + +D++ +EW + FPKI V+ R +I+I+ C VIL+ TN ++ DAY+ DKE I+ +L +A MK +K +S + + S S N +P+I++L+++S+VQYLD +++ + +L +PFA G AF S+LDSLMSTAY+N+NA+ ++ ++ GG+ +L++ + +T N ++ R R+ +P S++ + N Y +F++ ++ + GLYR N+ + T +R+V+TNP P P+ D V+CL+
Sbjct: 115 NLDWIIEA-------KDWAGEIISGQNGTGRVLISIVSALSLGSLIIYFIEISQLQGVTDELCIKWSESYTQQFDLAFNIVFLIFFCLRWIAATDKFWFWIEIYSLVDVLTVPPAFMTFVLNRYWVGLRFTRALRLISFTDVLVFLNILKSSSKIRLCHLVTLFTSLVLTSAGFIHLIELTGDIPEQT---NKQNKTYFDYTYFMIATMSTVGYGDIVCKTWQGKLMMIFFILGGLAIFANSIPEIIEIIGNRNRYGGIFKKEIGKQHIILCGHITF-------------QRTNCD-------SGHPSTEY-SIAK-----------CVEKKFKPARVQLEYADACLILCDKYSMDPDAEDAANIMRVISIKNYCPTIKVIVQLMQYHNKAYLMNIPSWDWRRGDDVVCVAELKLGFIAQSCLAPGFSTLLANLFVMRSFKKYHHLP---EWVNQYVEGASLEMYTEYLSPSFHGLTFTEAVCVCFTKLNLLLIAVEVKSNNRRNHSSSIAINPKPDITIEPGTQGFFIAESADDAKRAFYYCMACHMSCK---SVKPCNCQSSQHFQDTLYNIVSCSWQMFNNNGKSLIERNSFSKPETVIRNRRKRFGGQSAKRRSQALNDTIHHIKFNEQSQRRFDSTGMFHWCSNREFESAILTNKDDLSSTVFSNHIVVCIFADANSPLIGLRSFVMPLRSSNYHYNELRQIIFVGPQDYLRREWKSLMNFPKIFVLNGSPLNRSKLRMININLCEMTVILS--TNHSNGLVDAYLADKEAILCSLNIKA------MKFTDDNLQSKDLS-------EIFKNLSTSASHQNSI----------IPMITELSVDSSVQYLDQDDEDNPDTELFLTQPFACGRAFAVSVLDSLMSTAYFNENALTLIRTMVTGGATLELEELLSEGSGIQGAYLTPETVANRDRCRVETMRLENSPYSEL-----FGNEKTYGSLFMKCFKEYGILCFGLYRLIIDNMEDCSYSISTNKNRFVVTNPSPETPLMNQDWVFCLR 1062
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Match: SMESG000078105.1 (SMESG000078105.1) HSP 1 Score: 437.958 bits (1125), Expect = 3.023e-135 Identity = 311/1012 (30.73%), Postives = 500/1012 (49.41%), Query Frame = 2
Query: 380 MNIECPTQ---RKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTNFETACRSQKNLESLIEDPSFSVAFRS--YVNQLISTQFLIGRIVASIATVAALLSTVLYLYEIICLPLK------LEKCYPLT-NPIFCSDLACNFVFLIYFLLRFFTSTEKLAFWLGFHTVIDCFTIPQILTALVLDQEWIGLRFIRVLSLIRVFEVIQCFNVVKSGTSIRVIQLITYFASILIVGAGFFHLLENSGEITSGSFYNNSQPIGYGDSLYFIIVTMSTVGYGDIFAKTMCGKIFVVLFILLALATFASFIPELAEFLFAGSQYLGFYIKIAGRRHIIVCGHINYASVSTFLTDFLHEDRSSSDVDVIFINKKEPDIEMKSLLKIYFTQVKYFQGTVMDQVDLDRVQMSNADACLILADQNCSDPNDEDAGNIMRAISFKNYCYRCPVIIQLLQFENKTYLQNIPTWNKRNGDQIICYSEIELGILAQSCLVPGFSTLLTNIFSVRSEKECKQNPSNESWLNDYMRGVTMEIYSAKLSRSFNEFSYKELVEFCFKNLNLLLIAIEVKDSVSKKKM-LLMNPLNCKRIIPGSV-GFFICDSYRDALNANIYCSRCHATLRDLTNLKVCGCKGSPDDYELTVISRLKSEEEVSLTKCDVENDFKEMEFIDEKCCSYKKNGNFYTCSPQKLMKNVISSEHQIENLRGHIILCIFSDNRTSSIGIRNFLMPLRSVTISENELKELVIIADKDFIEKEWSEICGFPKIKVVFHQISIRRIAHLISIDTCSRCVILNNKTNIADLQNDAYMVDKEIIIANLFFQAESKRRIMKNNSKIEMAKSISIKAAGGETQIERTNQSKSEGNETFQVSKGSETQVPIISKLAIESNVQYLDVREDNVLNIPNYLRKPFAFGCAFTTSMLDSLMSTAYYNQNAIYIMEMLINGGSVDDLDQDDNDTEINLEQRNNQFRINLTPVSQINKTVNWSNTVFYRDIFIELMSKHESVSLGLYRKRNL 3373
M I C + KW F+ D L Y GGLL+L + + H K + + F+ ++ N + P+ +V R Y+ Q G +I LL+ VL L+ LE C P N + L + FLI FL+ F + L FW + +I+ FT+P + L + ++G F+R + + ++ +VIQ ++++ + IR+ L + F I + GAG F+L EN+G+I Y NSQ I YG YFI+VTMSTVGYGDI+ KT G+IF+ LF+L+ALA FAS+IPE+ + L + S Y + + ++H IVCG + + +FL+ F HE+R+S ++ +IFI+ P + +K L+K Y QV+Y +GT M DL R+++ AD CL++ +NC + + ED NIMR IS K C VIIQL QF+NK +L NIP W GD++IC++EI+LGILA+SCL GFST + + F++ S K +N N W+ Y RG + IY + S+ F ++ E V C+ +++I +EV DS + + L NP K +I + I ++ N +IYC RCH+ LK C C+ + ++V LK SL + KEME I K + F S + + + + + L HI+L +FS + S G+ NF+ PLR ++K ++++ + ++EKEW I FP+I VV + L ++ CV++N D Y+VDKE+I+ L K + + + S++ K II+ ++I++N+ +L + Y K FA G A+ S+L+SL+S+ YY+ N I ++ + I G D ++ + + ++ L S + +N N + ++F ++++ + +G+YR NL
Sbjct: 1 MKINCYEECNDNKWPIFLGFDCLIYIGGLLILFLKSKLSKLVHSK---VEMAKEFQVYLGTENNDYHM--QPNETVLTRPVRYIGQ--------GTFYGTIT----LLNVVLNLFSFFIYVFNTNEYYILENCCPWNENLLEIIQLVLSIFFLISFLIDFSMQKDHLEFWKDLYAIINIFTVPNTFIGIYLQRNYVGTFFLRFIWVYKIPDVIQQLDLLEVKSVIRLWHLGSIFMCIWVCGAGLFYLFENTGDIWHN--YKNSQKISYGACFYFILVTMSTVGYGDIYPKTDSGRIFLCLFLLIALAIFASYIPEMWDILKSRSIYDKTFRPVYNKKHNIVCGSLEDQEIESFLSTFYHENRNSKNIRIIFISLNRPKLYLKGLMKRY-PQVEYVEGTEMKSEDLKRIRVEIADCCLVITKRNCENEDAEDKANIMRVISIKECCPNQRVIIQLHQFQNKIHLLNIPNWCPLIGDEVICFNEIKLGILARSCLCAGFSTAICHAFTILSPKHIIKN-ENNPWIEQYKRGFSASIYRERFSQFFYGRTFLETVHICYDRFKIIVIGLEVIDSYGESTIKLYPNP---KHVITKQCWAYCISENQILPYNMSIYCKRCHSDKNSNLYLKKCYCEIITINSNMSVKIPLKQ----SLLAHN-----KEMEKILNK----NLDNKFEITSRRSFYHSFVKYD---KTLFDHIVLLVFSHENSKSAGLINFVKPLRIKKDDYVKIKPIILVGNGVYLEKEWKTISIFPEIYVVVGSCFDLEVLDLAQVNRARTCVLINAPKPFNHKVEDPYLVDKEVILGTLNLLNYLKYNKVSLSESDFSSSSVNTK---------------------------------IITDVSIDTNMHFLYPNSLDSSQNGAYFTKVFASGHAYCNSILNSLLSSTYYDWNTINLLRLFIEGSYDDSFEK--------VPGKYWHTQLKLISFSS-ERFLNLRNCKTFDEVFQTALNQNNILIIGIYRLFNL 930 The following BLAST results are available for this feature:
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calcium-activated potassium channel slo-1, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014811 ID=SMED30014811|Name=Calcium-activated potassium channel slo-1, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=3736bpback to top protein sequence of SMED30014811-orf-1 >SMED30014811-orf-1 ID=SMED30014811-orf-1|Name=SMED30014811-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1054bp MNIECPTQRKWYWFIAVDSLAYFGGLLLLVIWKIIINAFHRKRLKISQSTback to top protein sequence of SMED30014811-orf-2 >SMED30014811-orf-2 ID=SMED30014811-orf-2|Name=SMED30014811-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=208bp MATHNDMSAAGYFNIKTKILGSSKEKFCQFWNKASKRSQRQQNKQNDKDFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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