SMED30014784

Overview
NameSMED30014784
Smed IDSMED30014784
Length (bp)1623
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014784 (SMED30014784) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014784 (SMED30014784) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014784 (SMED30014784) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014784 (SMED30014784) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014784

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30014784SMESG000008843.1 SmedASXL_011698SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30014784SMESG000008843.1 SmedASXL_011698SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30014784SMESG000008843.1 dd_Smed_v6_1382_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30014784SMESG000008843.1 SMESG000008835.1 OX_Smed_1.0.21355ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014784 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005992:20717..22385 -8049
v31.005992:21800..23415 -2967
v31.005992:21539..23159 -2881
v31.005992:21234..22901 -2869
v31.005992:21067..22643 -2703
v31.005992:22013..23674 -2364
v31.005992:22298..23900 -1900
Homology
[Close]
BLAST of SMED30014784 vs. TrEMBL
Match: A0A1S6KMJ1 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 284.263 bits (726), Expect = 2.886e-84
Identity = 181/410 (44.15%), Postives = 271/410 (66.10%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR---VTKFTSRRXXXXXXXXXXLPG-------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEK 1189
            +V IKN K  E NLK I+ETSNK+I+ L E+NWE LVKYID+S+R EVI KI TFIT V+NKQYEKID +LINW+  II   R+V +VH KNEKI+ +N K+IT+TSNKY++ L E  WE L+K I+E+ RPEV  KIK FI  V+NK+YEKID+ L+N I +I+ TL++V+ I I+NEK      + +IK ++ Y+ +LNE+KWEELM+YIDE SRPEIIKKI  F+   +NK YD ID  L+N++ QII  L++IR   +T   ++++++    ++ +          W EL++ I   +    + +IK  +  +++K++++ID    +WI  +   +  + +  IK ++    N+  I +  +  V  L + KWE  + YIDE NR +  K ++  I++V+++Q+EK
Sbjct:  244 EVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVK-IKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEK 652

HSP 2 Score: 264.618 bits (675), Expect = 9.514e-77
Identity = 200/465 (43.01%), Postives = 283/465 (60.86%), Query Frame = 2 
Query:   29 TEENL---KTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGN---------WVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLK-SNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKIND-AITNGYVFDSAKK--TWVKNIVVNSRMRMKRSAGLNYMAE 1366
            TEEN+   KTIT+ SN ++  L EL WE L+KYIDESSRPEV+ KI TF+TLV+NKQYEKID+DLI W+T II TL+K  +V  KN K  + NLK I+ETSNK+ID L E  WE L+KYID+SNR EVI+KIK FI +VKNK+YEKIDT LIN+I  I+   +QV+ +HIKNEKI  +N  +I  TS KY+  L EL WEEL++ I+E  RPE+ KKI  FI+ V+NK Y+ ID +L+N I +II TL+++++     +     ++  IKL  +         W EL++ I        I +IK  + + ++K+Y +ID    +W+ ++  ++  I E  I        N   I +L    ++ L E KWE  + YID  NR +V   ++  I  V+++QFE+++  L+NW  +I     ++    I N  V    KK  T   N  VN  + +K    + Y+ E
Sbjct:  164 TEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVKIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDE 628

HSP 3 Score: 167.933 bits (424), Expect = 3.231e-41
Identity = 135/405 (33.33%), Postives = 236/405 (58.27%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXX----------LPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA-ITNG 1267
            NW  ++       R + I +I T I LV+  + +KID   + WL   ++ + KV ++ +  E I   ++K IT+ SN ++  L E +WE L+KYIDES+RPEV++KIK F+  V+NK+YEKID  LI +I +I+ TLK+ + + IKN K ++ N+  I +TS K+I +L E+ WEEL++YID+++R E+I KI  FI+ VKNK Y+ ID +L+N+I  IIT  ++++     + +     +  I           +  NW EL+++I+         +IK  ++++++K+Y+KID    + I E+   +  +  +    K  +   E  I    S ++ L E KWE  + YIDE +R ++ K ++  + + +++++++++  L+NW  QI  +  +I +  ITN 
Sbjct:  109 NWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKID---LTWLKE-LEKIFKVKEIPSTEENII--DIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVKIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNT 507

HSP 4 Score: 108.227 bits (269), Expect = 4.763e-21
Identity = 120/422 (28.44%), Postives = 218/422 (51.66%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITN 1264
            +K IT+   +  N L + NW  L+K ID   R    K+I   + LV+  ++++ID +L   +  I   +RKV +     +K+ +  ++I  E S + +  +    W  ++      +R + ID+I   I  VK  E +KID   +  + +I   +K++ +           ++  I   S  +++ L ELKWEEL++YIDE+SRPE++ KI  F++ V+NK Y+ ID+DL+ +I +IITTL+K +  +  + + + +   +I              NW EL++ I   +    I +IK  ++ +++K+Y+KID    +WI  +  I   + E  IK +K    N   I    +  V  L E  WE  +  I+E+ R +V K ++  I++V+++Q+EK++ +L+N   +I     ++   I N
Sbjct:   10 VKFITKDIKRITNRLIKWNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIK-----DKLPKVEIQIQKENSLEKLKPIITQNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTWLKELEKIFK-VKEIPSTE-----ENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVKIKIEN 420
[Close]
BLAST of SMED30014784 vs. TrEMBL
Match: A0A1S6KMJ8 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.632e-15
Identity = 85/390 (21.79%), Postives = 197/390 (50.51%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVN---YIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA 1255
            NWE L++   ++ +  + + I      V+    + +D +L+    +I + L K S++H +  +   +  ++  E  N  +++         +  +D   + E  DK+ K   +VKNKE+ K+D   +N    ++  L+    IH K    K+  V       + ++  L + KW EL++++  N +  + K +NE    +K + ++ I+++LV    ++ +++  L +I +   + ++ ++I+++++ L  +W  ++  ++G+D + WI R  KL ++++ K Y KID +W+ E + II     +           +  I K++  ++ L+ W W++ +  ++E  ++ + K++  +  I++ E F ++ + L+  F+ IF+I  KI  +
Sbjct:  131 NWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSK-SRIHQQFIRTQDQWTELTDEFGNGQLESF--------ISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRL--KIHGKESYKKRLLVQ-----WQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKIDVNWLAEFKPIIDKFRGKIKINYIH---QKRQIKKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQS 501

HSP 2 Score: 67.781 bits (164), Expect = 4.225e-8
Identity = 66/307 (21.50%), Postives = 152/307 (49.51%), Query Frame = 2 
Query:   71 YINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRT------IHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTS-----RRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDW 958
            ++ +L++  W  L+K++  + +  V K +     +++ +++EKI+++L+  +  ++  L+++ ++H K     + N+        + +  +   +W  ++  ++  +R + I++  K    ++ K Y KID   +N++A+    + + R       IH K +          IK    Y+ +L    W+ L+  ++E  +  I K+I      +K ++++ I+VDL+     I   L+KI+ +K+        ++ ++KKI++ +  NW+ ++  ++ VD   W  R  KL+ M+ +    KIDF W
Sbjct:  279 HVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVTNVKFVMRLLKRLWRIHIKYGSRKKANMI-------RRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNKID---VNWLAEFKPIIDKFRGKIKINYIHQKRQ----------IKKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMERIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNIKVTKEILLKKIKLIIDSNWLNIINGLEEVDKVKWTTRFNKLIQMIRNNNIDKIDFKW 565
[Close]
BLAST of SMED30014784 vs. TrEMBL
Match: A0A1S6KMJ4 (Uncharacterized protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 7.277e-10
Identity = 95/396 (23.99%), Postives = 193/396 (48.74%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTI-----IDTLRKVSQVHNK----NEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDL---VNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            NW+ LV   +  +RP++ K I        N   E  +  +  W  T        T RK  + + +     +++ +E+LK       +YID L+  +  IL K           +K+ K ++ +KNK++ KID  L+    QI+  L+            K+     I+    K++  L + KW +L+QY+ E  +  +   +NE IS VKN + + I+++L   VN+  +II  L KI +      + +II +I++ L  +W+ +L R++  + + WI R K+++ +++ K+Y ++D   +  L ++  +I +       + +++E  I  ++  VK L+EW W   + ++D+  R  +   + ++I IV+ +++++++   +     I +   KI
Sbjct:  132 NWQNLVDKSNNVNRPQMRKTIERVFKQTNNGGSELNEETVKQWKKTYKVLSRTRTHRKFIRTYERWNILTDEVGKEHLK-------RYIDKLDP-KLRILWK-----------NKLNKVMLLIKNKQFNKIDPKLLLKWKQIIYKLR-------LKINGKENYKQKILVNWRKHVDWLLKFKWTDLLQYVSEKYKIRVTNALNELISIVKNNDLNRINLELVDNVNFAIRIIKRLWKIHIVYGGREKAEIIHEIKLILTIDWLTILERLENKEKEKWIPRFKQVIEVIKRKKYNELD---LNLLLELKPIIKKIREVTNIKPLISERRINLIRRYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKI 498
[Close]
BLAST of SMED30014784 vs. Planmine SMEST
Match: SMESG000008843.1 (SMESG000008843.1)

HSP 1 Score: 957.977 bits (2475), Expect = 0.000e+0
Identity = 532/534 (99.63%), Postives = 532/534 (99.63%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRMRMKRSAGLNYMAESQAKMNVELLNQSIEALRKALMYNMMILSNNGQESVRKTDQNDNQSHLCPFIDKVNNKLTTKLTNCEMKQRFICKRQLN 1603
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFI LVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRKDIIKKIQIKLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWIEELQKIISLISEEEIKLKKEVILNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRMRMKRSAGLNYM ESQAKMNVELLNQSIEALRKALMYNMMILSNNGQESVRKTDQNDNQSHLCPFIDKVNNKLTTKLTNCEMKQRFICKRQLN
Sbjct: 3091 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRKDIIKKIQIKLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWIEELQKIISLISEEEIKLKKEVILNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRMRMKRSAGLNYMDESQAKMNVELLNQSIEALRKALMYNMMILSNNGQESVRKTDQNDNQSHLCPFIDKVNNKLTTKLTNCEMKQRFICKRQLN 3624

HSP 2 Score: 387.497 bits (994), Expect = 5.048e-119
Identity = 232/430 (53.95%), Postives = 303/430 (70.47%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD---WXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKIN 1249
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV QVH KNEK+++ENLK ITETSNKYI+ L E  WE+L+KYIDES+RPEVI KI  FI  V+NK+YEKID  LIN++  I+ TL++VR +HIKNEK+ +EN+  I +TS KYI  L EL WE L++YIDE+SRPE+IKKI  FI+ V+NK Y+ IDVDL+N++  II TL+K+R     + +        I              NW  L++ I        I +I   ++++++K+Y+KID D   W+  +   +  + +  IK +K    N  +I +  +  +  L+E  WE  + YIDES+R +V K +   I++V+++Q+EK+++ L+NW   I     K++
Sbjct: 2747 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVS 3176

HSP 3 Score: 350.132 bits (897), Expect = 4.114e-106
Identity = 201/328 (61.28%), Postives = 244/328 (74.39%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD 955
            QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV QVH KNEK+++ENLK ITETSNKYI+ L E  WE+L+KYIDES+RPEVI KI  FI  V+NK+YEKID  LIN++  I+ TL++V  +H KNEKI +EN+ +I +TS KYI  LNE +WE LM+YIDE++RPE+I KI +FI +VKNK Y+ ID  L+N+I QI+ TL+++R     + +        I               W EL++ I        I +I + +S +++K Y  ID D
Sbjct: 3005 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFIALVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVD 3332

HSP 4 Score: 317.005 bits (811), Expect = 1.028e-94
Identity = 200/429 (46.62%), Postives = 275/429 (64.10%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD---WXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            Q+HIKNE+VT EN K ITETSNKY+N+L +L WE L+KYIDE +RPE  KKI TFITLVQNKQYEKIDVDLINWLT II TLRK+ +VH KN K+++ENLK ITETSNKYI+ L E  WE+L+KYIDES+RPEVI KI  FI  V+NK+YEKID  LIN++  I+ TL++VR +HIKNEK+ +EN+  I +TS KYI  L EL WE L++YIDE+SRPE                   IDVDL+N++  II TL+K+R     + +        I              NW  L++ I        I +I   ++++++K+Y+KID D   W+  +   +  + +  IK +K    N  +I +  +  +  L+E  WE  + YIDES+R +V K +   I++V+++Q+EK+++ L+NW   I     K+
Sbjct: 2508 QIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEIIPTLRKIREVHIKNAKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPE-------------------IDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV 2917

HSP 5 Score: 299.671 bits (766), Expect = 1.077e-88
Identity = 192/446 (43.05%), Postives = 289/446 (64.80%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR---VTKFTSRRXXXXXXXXXXLPG-------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA-ITNGYVFDSAKKT 1294
            +V IKN K  E NLK I+ETSNK+I+ L E+NWE LVKYID+S+R EVI KI TFIT V+NKQYEKID +LINW+  II   R+V +VH KNEKI+ +N K+IT+TSNKY++ L E  WE L+K I+E+ RPEV  KIK FI  V+NK+YEKID+ L+N I +I+ TL++V+ I I+NEK      + +IK ++ Y+ +LNE+KWEELM+YIDE SRPEIIKKI  F+   +NK YD ID  L+N++ QII  L++IR   +T   ++++++    ++ +          W EL++ I   +    + +IK  +  +++K++++ID    +WI  +   +  + +  IK ++    N+  I +  +  V  L + KWE  + YIDE NR +  K ++  I++V+++Q+EK+++ L+NW  +I     KI +  I N  V +   KT
Sbjct: 2165 EVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVK-IKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEIIPTLRKIREVHIKNAKVTEENLKT 2609

HSP 6 Score: 292.738 bits (748), Expect = 2.387e-86
Identity = 184/420 (43.81%), Postives = 269/420 (64.05%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGN-WVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD---WXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +++I N    ++NLK ITE S +YI  L EL WE L+KYID  +RPEV+ KI TFI  VQNKQ+E+ID  L+NW+T II TLR+V Q+H KNE+++ EN KIITETSNKY++ L + +WE L+KYIDE NRPE   KIK FI  V+NK+YEKID  LIN++ +I+ TL+++R +HIKN K+ +EN+  I +TS KYI  L EL WE L++YIDE+SRPE+IKKI  FI+ V+NK Y+ IDVDL+N++  II TL+K+R     + +        I    N ++  LR +       W    + L+  ++     +ID D   W+  +   +  + +  IK +K    N  +I +  +  +  L+E  WE  + YIDES+R +V K +   I++V+++Q+EK+++ L+NW   I     K+
Sbjct: 2422 EINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEIIPTLRKIREVHIKNAKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELN------W----EVLVKYIDESSRPEIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV 2831

HSP 7 Score: 263.077 bits (671), Expect = 3.481e-76
Identity = 188/416 (45.19%), Postives = 265/416 (63.70%), Query Frame = 2 
Query:   29 TEENL---KTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGN---------WVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLK-SNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIF 1228
            TEEN+   KTIT+ SN ++  L EL WE L+KYIDESSRPEV+ KI TF+TLV+NKQYEKID+DLI W+T II TL+K  +V  KN K  + NLK I+ETSNK+ID L E  WE L+KYID+SNR EVI+KIK FI +VKNK+YEKIDT LIN+I  I+   +QV+ +HIKNEKI  +N  +I  TS KY+  L EL WEEL++ I+E  RPE+ KKI  FI+ V+NK Y+ ID +L+N I +II TL+++++     +     ++  IKL  +         W EL++ I        I +IK  + + ++K+Y +ID    +W+ ++  ++  I E  I        N   I +L    ++ L E KWE  + YID  NR +V   ++  I  V+++QFE+++  L+NW  +I 
Sbjct: 2085 TEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVKIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRII 2500

HSP 8 Score: 259.61 bits (662), Expect = 5.748e-75
Identity = 165/396 (41.67%), Postives = 252/396 (63.64%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD---WXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSN-VKQLQEWKWESYLAYIDESNRQQVEKDV 1147
            ++ I+NEK      + + + ++ Y+  L E+ WE L+KYIDE SRPE+IKKI  F+ L QNK+Y++ID  L+NWL  II  LR++ +++  N    Q+NLK+ITE S +YI  LNE +WE L+KYID  NRPEV+DKIK FI +V+NK++E+IDTGL+N+I +I+ TL++V+ IHIKNE++  EN  +I +TS KY+  L +LKWEEL++YIDE +RPE  KKI  FI+ V+NK Y+ IDVDL+N++ +II TL+KIR     + +        I              NW  L++ I        I +I   ++++++K+Y+KID D   W+  +   +  + +  IK +K    N  +I +  +  +  L+E  WE  + YIDES+R +++ D+
Sbjct: 2336 KIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNTNNKQQNLKVITELSIQYIQLLNELKWEELIKYIDAKNRPEVVDKIKTFIKFVQNKQFEQIDTGLLNWITRIITTLREVKQIHIKNEEVTTENKKIITETSNKYVNYLIDLKWEELIKYIDEKNRPEFTKKIKTFITLVQNKQYEKIDVDLINWLTEIIPTLRKIREVHIKNAKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVRQVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEIDVDL 2731

HSP 9 Score: 167.162 bits (422), Expect = 1.425e-43
Identity = 135/405 (33.33%), Postives = 236/405 (58.27%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXX----------LPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA-ITNG 1267
            NW  ++       R + I +I T I LV+  + +KID   + WL   ++ + KV ++ +  E I   ++K IT+ SN ++  L E +WE L+KYIDES+RPEV++KIK F+  V+NK+YEKID  LI +I +I+ TLK+ + + IKN K ++ N+  I +TS K+I +L E+ WEEL++YID+++R E+I KI  FI+ VKNK Y+ ID +L+N+I  IIT  ++++     + +     +  I           +  NW EL+++I+         +IK  ++++++K+Y+KID    + I E+   +  +  +    K  +   E  I    S ++ L E KWE  + YIDE +R ++ K ++  + + +++++++++  L+NW  QI  +  +I +  ITN 
Sbjct: 2030 NWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKID---LTWLKE-LEKIFKVKEIPSTEENII--DIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDLITWITEIITTLKKAKEVQIKNTKTEEVNLKEISETSNKFIDILVEINWEELVKYIDQSNRLEVINKIKTFITFVKNKQYEKIDTNLINWINHIITIFRQVKEVHIKNEKITNDNRKLITDTSNKYVNFLIELNWEELVKQIEETKRPEVTKKIKTFITLVQNKQYEKIDSELLNLIVEIINTLREVKIKIENEKSNLSYRERVIKLTDSYLEILNEVKWEELMKYIDEQSRPEIIKKIKNFVILAQNKKYDEIDSTLLNWLNQIIVMLRRIREINITNT 2428

HSP 10 Score: 95.9005 bits (237), Expect = 2.653e-20
Identity = 98/407 (24.08%), Postives = 206/407 (50.61%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +++  +++  T        +   + VI K  +K++  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  990 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKAT-------KRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1381

HSP 11 Score: 94.7449 bits (234), Expect = 6.840e-20
Identity = 98/407 (24.08%), Postives = 206/407 (50.61%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +++  +++  T        +   + VI K  +K++  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct: 1451 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKAT-------KRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1842

HSP 12 Score: 94.3597 bits (233), Expect = 9.054e-20
Identity = 102/420 (24.29%), Postives = 209/420 (49.76%), Query Frame = 2 
Query:    2 QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            ++  +N K+    L  IT    + +N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +I+  +K+       N   K   V +I K  +++I  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  497 KIKYRNNKIKPLTLNIITRNVKRLVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKK-------NAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 901

HSP 13 Score: 92.0485 bits (227), Expect = 4.493e-19
Identity = 97/407 (23.83%), Postives = 205/407 (50.37%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +++  +++  T        +   + VI K  +K++  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  670 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKAT-------KRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1061

HSP 14 Score: 87.8113 bits (216), Expect = 8.966e-18
Identity = 104/400 (26.00%), Postives = 195/400 (48.75%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTS-----RRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQ-------LQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQL 1204
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +I+  +++  T        K   V  I K  ++    L +  W +L++ ID   R    K+I+  +  VK   +  ID +L   I  I   ++K+   K        ++++ ++K++  +  NW +++   K +D   WI RI  ++ +++  E  KID  W         L   E+I   KE+   E +I  +K+   +       L E KWE  L YIDES+R +V   ++  +++VE++Q+EK++I L
Sbjct: 1771 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNKIINVIRKNTT-------EKPITVKFITKDIKRITNRLIKWNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEIDENLYKKIRVIYKKIRKVIKDKLPKVEIQIQKENSLEKLKPIITKNWAKIINETKNIDRVKWIDRINTIIKLVKLNEVDKIDLTW---------LKELEKIFKVKEIPSTEENIIDIKTITDKSNNFLITLTELKWEELLKYIDESSRPEVLNKIKTFVTLVENKQYEKIDIDL 2149

HSP 15 Score: 87.8113 bits (216), Expect = 1.049e-17
Identity = 98/402 (24.38%), Postives = 197/402 (49.00%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +++  +++  T        +   + VI K  +K++  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+      +R  I  K          +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct: 1150 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKAT-------KRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIK----NDKRPIIDIK----------KVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1522

HSP 16 Score: 87.0409 bits (214), Expect = 1.896e-17
Identity = 99/411 (24.09%), Postives = 208/411 (50.61%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAI 1258
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID   +    +++  +++  T        +   + VI K  +K++  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  KII++I +   +    V      I ++ +   +L +W W   +  ID   R++ +K++  ++ +V+  +F++++    N +++I  I+ KI   I
Sbjct: 1611 IKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKEKLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKAT-------KRPITIKVITKDVKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNKIINVIRKNTTEKPITVKFITKDIKRITN---RLIKWNWPDLIKNIDVKERERFQKEIDIVLKLVKENKFKEID---ENLYKKIRVIYKKIRKVI 2003

HSP 17 Score: 72.7886 bits (177), Expect = 4.243e-13
Identity = 74/303 (24.42%), Postives = 149/303 (49.17%), Query Frame = 2 
Query:   68 KYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELK------WEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXX---XXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID 949
            +Y+  L+E NW  L+K++D+  R  +I  I   I +V  K+Y++ID + I  L  I+  +RK+     +N KI    L IIT    + ++ L ++ W  L+K IDE +R  V  +I+  +  VK  ++++ID  L   I  I  T+++++         K+  +D+ ++       +LN+LK      W++++       + +   +IN+ I  +K    D ID+  +    +II  ++K  + K  + +          +  +  NW +L++ I   D +     I+ +L +++  ++++ID
Sbjct:  436 RYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKIKY---RNNKIKPLTLNIITRNVKRLVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKN-------DKRPIIDIKVQKER----LLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEID 724

HSP 18 Score: 71.2478 bits (173), Expect = 1.322e-12
Identity = 79/302 (26.16%), Postives = 164/302 (54.30%), Query Frame = 2 
Query:  359 EILMKYIDESNRPEVI---DKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDL---VNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            E L +YID+ +    I   +K+ K ++ +KNK++ KID  L+    QI+  L+    I+ K    +K  V+       K++  L + KW +L+QY+ E  +  +   +NE IS VKN + + I+++L   VN+  +II  L KI +      + +II +I++ L  +W+++L R++  + + WI R K+++ +++ K+Y ++D   +  L ++  +I +     K + +++E  I  ++  VK L+EW W   + ++D+  R  +   + ++I IV+ +++++++   +     I +   KI
Sbjct:  207 EHLKRYIDKLDPKLRILWKNKLNKVMLLIKNKQFNKIDPKLLLKWKQIIYKLRL--KINGKENYKQKILVN-----WRKHVDWLLKFKWTDLLQYVSEKYKIRVTNALNELISIVKNNDLNRINLELVDNVNFAIRIIKRLWKIHIVYGGREKAEIIHEIKLILTIDWLKILERLENKEKEKWIPRFKQVIEVIKRKKYNELD---LNLLLELKPIIKKIREVTKIKPLISERRINLIRRYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKI 498

HSP 19 Score: 55.0694 bits (131), Expect = 1.443e-7
Identity = 57/239 (23.85%), Postives = 117/239 (48.95%), Query Frame = 2 
Query:   68 KYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLI---NWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR 775
            K++++L +  W  L++Y+ E  +  V   +   I++V+N    +I+++L+   N+   II  L K+  V+   EK       II E     I  +   +W  +++ ++   + + I + K+ I  +K K+Y ++D  L+  +  I+  +++V  I     + +       I    +Y+ +L E  W ELM+++D+  R  +I  I + I  V  K Y  ID + +  +  I+  ++KI+
Sbjct:  278 KHVDWLLKFKWTDLLQYVSEKYKIRVTNALNELISIVKNNDLNRINLELVDNVNFAIRIIKRLWKIHIVYGGREKAE-----IIHE-----IKLILTIDWLKILERLENKEKEKWIPRFKQVIEVIKRKKYNELDLNLLLELKPIIKKIREVTKIKPLISERR-------INLIRRYVKLLKEWNWPELMKFVDQKERNNLIIGIEKMIGIVDQKRYKEIDNNFIQKLRFIVRFMRKIK 499
[Close]
BLAST of SMED30014784 vs. Planmine SMEST
Match: SMESG000008835.1 (SMESG000008835.1)

HSP 1 Score: 412.92 bits (1060), Expect = 7.482e-128
Identity = 255/462 (55.19%), Postives = 333/462 (72.08%), Query Frame = 2 
Query:    8 HIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRV--TKFTSRRXXXXXXX-XXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRM-RMKRSAGLNYMAESQAKM 1381
            H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLR   + H  NEK    NLK ITETS KYI++L+E +WE L KYIDE NR  VIDKIK FI +V+NKE+E ID  L+N+I +I+ TL++ R I+  NEK+K +N+  + +TS KY   L ELKWEEL+Q+ID +SR E+I KI  FIS V+NK++D IDV+L N+I +II  L+KIR+  +K T  +   I +  Q+ LP NW ELL+RI G+D+ LW  RI +LL++M+SK +QKIDF+WI EL+KI++LIS+E IK+KKEV LNE SI KLK  V QL+EW W S L YIDE+NRQQ++ +++ +IS+VES  FEKLEIQ VNWF QIF+ F+KI + + NGYV+DS  KTWVK+I  N  + R +R   LN +  +  K+
Sbjct: 2109 HVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKAREINETNEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKIRIGQSKVTENKTLKITENIQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQESIKMKKEVFLNETSIEKLKGFVSQLKEWNWNSLLIYIDENNRQQIQNEIQGLISVVESGNFEKLEIQTVNWFYQIFETFSKIKEFMNNGYVYDSKIKTWVKHITQNQNIVRKRRCILLNLLHGAAKKL 2570

HSP 2 Score: 313.153 bits (801), Expect = 2.046e-93
Identity = 196/456 (42.98%), Postives = 308/456 (67.54%), Query Frame = 2 
Query:  248 RKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFT----SRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRMRMKRSAGLNYMAESQAKMNVELLNQSIEALRKALMYNMMILSNNGQESVRKTDQNDNQSHLCPFIDKVNNKLTTKLTNCEMKQRFICKRQLN 1603
            +K+ Q H++  KI  +  K I     K ++ L ++ W+ LMKY+ ++++ + + KIK  I  +KNK+Y+ ID  L+ +I+ I   LK VR I  + + I +  + +++K S K++ +LN+L+W +++  I+ +++PEI +KI  FI+ V+N+ YD ID + +N++ +II  ++K +  K T    ++   +I+KIQI LP NW ELL+ ++G+D +LW  RI+ LLS+++SKEYQKIDF+WI+ELQKI+ LIS +  + K++ +L E +  K+K N+  L++W WES L YI+E+NR+QVE DV+++I IVE+E+F+K++ Q V WF QI ++F KIN AITNGYV++S  KTWVKN+V  S  R +RS  LN    S   ++ +L+ + IE +++AL YN+MIL  N   S    + ND  S  CPFI+KVN  L TK TNC++KQ+FICK  +N
Sbjct: 3162 KKIHQKHHR--KIVSKERKRIVHKVIKRVNWLEKWNWKNLMKYVVDNHKRDTLVKIKYIISLIKNKDYDNIDEDLLKWISTIKTKLKIVRKIKKETKNIDEN-LKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIENIKKTK-QKLTETDNTKNVKVIEKIQINLPSNWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIVQLISTQVTESKEKFVLPESTKTKIKENINSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKIDSQFVQWFNQIVELFNKINYAITNGYVYNSETKTWVKNVVEESLTRKRRS--LNQNKFSDLNLSTDLITKVIEQVQRALSYNLMILRGNNLNS-NGAESNDRPSDRCPFINKVNENLVTKFTNCDIKQKFICKMSMN 3610

HSP 3 Score: 268.085 bits (684), Expect = 8.203e-78
Identity = 181/418 (43.30%), Postives = 253/418 (60.53%), Query Frame = 2 
Query:   35 ENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD---WXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLK-SNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +N+K+IT+ S K+I+ L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLRK  + H  NEK    NLK ITETS KYI++L+E +WE L KYIDE NR  VIDKIK FI +V+NKE+E ID  L+N+I +I+ TL+  R  H+ NEK K  N+  I +TS KYI  L+ELKWEEL +YIDE +R  +I KI  FI  V+NK ++NID+DL+N+I +II TL+  R +  T+ +        I               W EL + I   +    I +IK  +  +E+KE++ ID D   WI E+ K +    E     +K  I N  ++ +        L E KWE  + +ID S+R +V   ++  IS V+++ F+K+++ L NW  +I  I  KI
Sbjct: 1946 KNIKSITDKSLKFIDSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKAREINETNEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKI 2363

HSP 4 Score: 257.299 bits (656), Expect = 3.239e-74
Identity = 174/422 (41.23%), Postives = 250/422 (59.24%), Query Frame = 2 
Query:    8 HIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXX----------LPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTK 1243
            H+ NEK    NLKTITETS KYIN L EL WE L KYIDE +R  VI KI TFI  V+NK++E ID+DL+NW+  II TLR   + H  NEK    NLK ITETS KYI++L+E +WE L KYIDE NR  VIDKIK FI +V+NKE+E ID  L+N+I +I+ TL+  R  H+ NEK K  N+  I +TS KYI  L+ELKWEEL +YIDE +R  +I KI  FI  V+NK ++NID+DL+N+I +II TL+K R    T+ +  I     +           +   W EL++ I        I +IK  +S +++K + KID +       I  +I+        +  + E    K+  N++      W+  L  I+  + Q     + ++++I++S+  +K++ + +   E+I  + ++
Sbjct: 2023 HVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKAREINETNEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVN---LANWIAEIINILRKIRIGQSKVTENKTLKITENIQLTLPSNWQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQ 2441

HSP 5 Score: 216.083 bits (549), Expect = 4.557e-60
Identity = 155/440 (35.23%), Postives = 246/440 (55.91%), Query Frame = 2 
Query:   74 INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQ--KIRVT-KFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIV-VNSRMRMKRSAGLNYMAESQAKM 1381
            IN L+  NWE  ++YIDE  R  +   I   I +++N + +K+D D I+W   I +  +K++                     N Y+   N   W          N  EV +KI+K                L N        L ++          +++ ++ II  S KY+V+LNEL+W EL+  ID  ++  I  KIN+FI +V  + Y  I+   +N++ QII TLQ  KIR+T     +R +I KK+ + LP NW E++ ++  ++ DLWI RI+ LL M+E  E+ KIDF WI+ELQK++ LI+EE IK  ++  +NE +I  +K NV  L++W WE+YL YIDE  R++V+++V  +I+I+E+ +++KL+++ V+WF QIF++F K+  A TNGYV+D   K+WVK +V V +  R +R   LN +  +  K+
Sbjct: 2603 INSLKLWNWENFLQYIDEDKREPIQTNINKIIYVIENGESDKLDPDTIDWFIQIFEEFKKINYA-----------------IENGYVYDPNTRTWT--------KNTVEVENKIRK-------------KRCLFNIFKGFHRLLPRISIRGRIGHVNREDKLNFIIDMSHKYLVILNELRWNELIDSIDGFNKRSIFVKINKFIHYVNTRKYHKINRKFLNWLSQIIITLQNTKIRLTGSMKQKRIEIAKKVLVNLPSNWKEVIVKLPNINVDLWIKRIQLLLPMIELNEFDKIDFGWIDELQKLVDLITEESIKTVEKPKVNEVTIKTVKENVNSLKQWNWETYLKYIDEDKREEVQRNVNNVITIIENVEYDKLDVKSVDWFNQIFEVFNKMYYATTNGYVYDEKTKSWVKTVVEVENNTRKRRCILLNLLHGAAKKL 3004

HSP 6 Score: 191.43 bits (485), Expect = 1.132e-51
Identity = 153/455 (33.63%), Postives = 247/455 (54.29%), Query Frame = 2 
Query:   17 NEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKV----SQV-HNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVE-------LLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIV-VNSRMRMKRS 1342
            NEKV  +NLKT+TETS KY N+L EL WE LV++ID SSR EVI KI TFI+ VQNK ++KIDV+L NW+  II+ LRK+    S+V  NK  KI+ EN+++ T  SN          W+ L++ I+  +      +I + +  +K+K ++KID   I  + +I+  + Q         K +    +  I+  + ++  L E  W  L+ YIDEN+R +I  +I   IS V++ N++ +++  VN+  QI  T  K  + +F +       KI       WV+       ++R+ + +  +L  G  KKL+  +                      ++      +     N+ SI  +++N+  L+ W WE++L YIDE  R+ ++ ++ +II ++E+ + +KL+   ++WF QIF+ F KIN AI NGYV+D   +TW KN V V +++R KR 
Sbjct: 2284 NEKVKIKNLKTLTETSLKYFNYLIELKWEELVQHIDSSSRVEVINKIKTFISFVQNKSFDKIDVNLANWIAEIINILRKIRIGQSKVTENKTLKIT-ENIQL-TLPSN----------WQELLQRINGIDYQLWTSRIDQLLTIMKSKNHQKIDFEWIGELEKIVNLISQ----ESIKMKKEVFLNETSIEKLKGFVSQLKEWNWNSLLIYIDENNRQQIQNEIQGLISVVESGNFEKLEIQTVNWFYQIFETFSK--IKEFMNNGYVYDSKI-----KTWVKHITQNQNIVRKRRCILLNLLHGAAKKLIPHIS-------------------IGLNIGGKPRRPRPNYNQVSIQTIRTNINSLKLWNWENFLQYIDEDKREPIQTNINKIIYVIENGESDKLDPDTIDWFIQIFEEFKKINYAIENGYVYDPNTRTWTKNTVEVENKIRKKRC 2696

HSP 7 Score: 174.096 bits (440), Expect = 7.561e-46
Identity = 134/393 (34.10%), Postives = 212/393 (53.94%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG----------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFD---WXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLK-SNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQ 1231
            NW  +++      + +   +I T   L+ +KQ +KID    +WL  +++      +    + +I   +L ++TE S   I  LN+  WE L+KYI E N+PEV++KIK FI  VKNK+++ ID   INFI +I++T K+++    + +    +N+  I   S K+I  L+ELKWEEL +YIDE +R  +I KI  FI  V+NK ++NID+DL+N+I +II TL+K R +  T+ +        I               W EL + I   +    I +IK  +  +E+KE++ ID D   WI E+ K +    E  +  +K    N  +I +     +  L E KWE    YIDE NRQ V   ++  I  VE+++FE ++I L+NW  +I +
Sbjct: 1802 NWTKIIEKSTNIDKTKWTTRIKTITELINSKQTDKIDY---SWLLELLEIFNFPDE---PDREIPTIDLAVVTEKSISLITDLNQLNWEELVKYIGEPNQPEVVNKIKTFIFNVKNKKFQNIDGETINFITEIISTFKKIK--ENRIKTENTKNIKSITDKSLKFIDSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRKARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIKTLRNARESHVTNEKTKTSNLKTITETSIKYINSLHELKWEELTKYIDEKNRQTVIDKIKTFIRFVENKEFENIDIDLLNWILEIIK 2186

HSP 8 Score: 119.398 bits (298), Expect = 7.158e-28
Identity = 103/323 (31.89%), Postives = 181/323 (56.04%), Query Frame = 2 
Query:    2 QVHIKNE-KVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNID---VDLVNYIGQIITTLQKIRVTKFT---SRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID 949
            ++H K+  K+  +  K I     K +N+L + NW+ L+KY+ ++ + + + KI   I+L++NK Y+ ID DL+ W++TI   L+ V ++  + + I  ENLKI+ + S+K++D LN+  W  ++  I+  N+PE+ +KIK+FI  V+N+EY+KIDT  +N++ +I+  +K  +T     E    +NV VI    EK  + L    W+EL+Q ++         +I   +S V++K Y  ID   +D +  I Q+I+T       KF    S +  I + I      NW  LL+ I+  +       +KK++ ++E++E+ KID
Sbjct: 3163 KIHQKHHRKIVSKERKRIVHKVIKRVNWLEKWNWKNLMKYVVDNHKRDTLVKIKYIISLIKNKDYDNIDEDLLKWISTIKTKLKIVRKIKKETKNID-ENLKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIENIK--KTKQKLTETDNTKNVKVI----EKIQINLPS-NWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIVQLISTQVTESKEKFVLPESTKTKIKENINSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKID 3477

HSP 9 Score: 97.4413 bits (241), Expect = 8.391e-21
Identity = 105/407 (25.80%), Postives = 210/407 (51.60%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID        + L   K++  I  KN   K   V +I K  +++I  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++ ++II+++ +  IK    V +   ++ +    +K+L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct: 1063 IKVITKDVKKFVNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKID-------LKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRF---IKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1454

HSP 10 Score: 95.9005 bits (237), Expect = 2.722e-20
Identity = 108/411 (26.28%), Postives = 206/411 (50.12%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXX-----XXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXX--XXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKIND 1252
            +K IT+   K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID        + L   K++  I  KN   K   V +I K  +++I  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I   ++K+++ K  + +K +     + K++  L  NW +++ +   +D   W  RIK +  ++ SK+  KID+ W+ EL +        + EI      ++ E SI+ L +++ QL    WE  + YI E N+ +V   ++  I  V++++F+ ++ + +N+  +I   F KI +
Sbjct: 1543 IKVITKDVKKFVNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKID-------LKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKKVRKVKIDKIPTEKKQLLKEKILTKLKPILNENWTKIIEKSTNIDKTKWTTRIKTITELINSKQTDKIDYSWLLELLEIFNFPDEPDREIPTIDLAVVTEKSIS-LITDLNQLN---WEELVKYIGEPNQPEVVNKIKTFIFNVKNKKFQNIDGETINFITEIISTFKKIKE 1937

HSP 11 Score: 94.7449 bits (234), Expect = 7.207e-20
Identity = 48/127 (37.80%), Postives = 83/127 (65.35%), Query Frame = 2 
Query: 1031 NEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIV-VNSRMRMKR--SAGLNYMAESQAKMNVELLNQ 1402
            N+ SI  +++N+  L+ W WE++L YIDE  R+ ++ ++ +II+++E+ + +KL+   ++WF QIF+ F KIN AI NGYV+D   +TW KN V V +++R KR  S G   + + +   N++  N+
Sbjct: 3026 NQVSIQTIRTNINSLKLWNWENFLQYIDEDKREPIQTNINKIINVIENGESDKLDPDTIDWFIQIFEEFKKINYAIENGYVYDPNTRTWTKNTVEVENKIRKKRCFSKGHRKIRKFRPHNNLKFHNK 3152

HSP 12 Score: 94.3597 bits (233), Expect = 8.663e-20
Identity = 102/407 (25.06%), Postives = 205/407 (50.37%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID        + L   K++  I  KN   K   V +I K  +++I  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  743 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKID-------LKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1134

HSP 13 Score: 94.3597 bits (233), Expect = 8.663e-20
Identity = 102/407 (25.06%), Postives = 205/407 (50.37%), Query Frame = 2 
Query:   41 LKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            +K IT+   ++I  L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID        + L   K++  I  KN   K   V +I K  +++I  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++  ++I ++ ++  K    + +    + K    V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct: 1223 VKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKID-------LKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFNRLIKIVRKKATKRPITIKVITKDVKKF---VNRLIKWNWSDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 1614

HSP 14 Score: 93.5893 bits (231), Expect = 1.531e-19
Identity = 102/398 (25.63%), Postives = 205/398 (51.51%), Query Frame = 2 
Query:   68 KYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            K++N L + NW  L+K IDE  R  V K+I   + LV+  ++++ID +L   +  I  T+RK+     KN+K    ++K+  E     +  + +  W+ ++      ++ +  ++I K I  +K  + +KID        + L   K++  I  KN   K   V +I K  +++I  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++ ++II+++ +  IK    V +   ++ +    +K+L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  592 KFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI-----KNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKID-------LKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRFIKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKKPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNVIKKPITVKIITKNVKRF---IKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 974

HSP 15 Score: 92.4337 bits (228), Expect = 3.738e-19
Identity = 74/253 (29.25%), Postives = 140/253 (55.34%), Query Frame = 2 
Query:   14 KNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKI 772
            K  K  +ENLK + + S+K+++ L +L W  ++  I+  ++PE+ +KI  FITLVQN++Y+KID + +NWL  II+ ++K  Q   + +  + +N+K+I +     I       W+ L++ ++  +      +I+  +  V++KEY+KID   I+ + +I+  +   +    K + +  E+    IK +   I  L +  WE L++YI+E +R ++   + + I  V+N+ +D ID   V +  QI+    KI
Sbjct: 3253 KETKNIDENLKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNWLFEIIENIKKTKQKLTETD--NTKNVKVIEK-----IQINLPSNWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIVQLI-STQVTESKEKFVLPESTKTKIKEN---INSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKIDSQFVQWFNQIVELFNKI 3494

HSP 16 Score: 85.8853 bits (211), Expect = 4.220e-17
Identity = 84/393 (21.37%), Postives = 197/393 (50.13%), Query Frame = 2 
Query:   95 NWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEY---EWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVN---YIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDA 1255
            NWE L++   ++ +  + + I      V+    + +D +L+    +I + L K S++H +              T +++ +  +E+   + E  +  +D   + E  DK+ K   +VKNKE+ K+D   +N    ++  L+    IH K    K+  V       + ++  L + KW EL++++  N +  + K +NE    +K + ++ I+++LV    ++ +++  L +I +   + ++ ++I+++++ L  +W  ++  ++G+D + WI R  KL ++++ K Y +ID +W+ E + II     +           +    K++  ++ L+ W W++ +  ++E  ++ + K++  +  I++ E F ++ + L+  F+ IF+I  KI  +
Sbjct:  131 NWEQLIENTKDTDQSTIKRNIKKIFEAVKTSGNKNLDPNLLKKWKSIYEILSK-SRIHQQ-----------FIRTQDQWTELTDEFGNGQLESFISTLDPKLQVEWKDKLSKVKWFVKNKEFNKLDLNSLNEWKNLIYKLRL--KIHGKESYKKRLLVQ-----WQSHVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVKNVKFVMRLLKRLWRIHIKYGSRKKANMIRRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNEIDVNWLAEFKPIIDKFRGKIKINYIHQKRQK---KKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMKRIIKEEHFNEINVDLIKRFKVIFKILRKIKQS 501

HSP 17 Score: 84.7297 bits (208), Expect = 1.024e-16
Identity = 95/397 (23.93%), Postives = 200/397 (50.38%), Query Frame = 2 
Query:   71 YINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIR-----VTKFTSRRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            Y+  L+  NW+ LV  ++E  +  + K+I     +++ + + +I+VDLI     I   LRK+ Q      K  + N+K+  E   K I  + +  W  ++  ++  ++ +   +  K I  ++N   +KID   +    +IL      + IH K+   K++     +   +K++  L +  W +L++ IDE  R  + K+I   +  VK   +  ID +L   I  I  T++KI+     +     +++ ++ K+++ +  NW +++   KG+D   W  RI KL+ +++  +  KID  W+++ ++II+++ +  IK    V +   ++ +    +K+L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  437 YLRLLKRWNWKNLVVDLNEKEKENIAKEIELMKRIIKEEHFNEINVDLIKRFKVIFKILRKIKQ-----SKYPKGNVKVTKEILLKKIKLIIDSNWLNIINGLEGVDKVKWTTRFNKIIQMIRNNNIDKIDFKWLLKFKRIL------KLIHRKSHPRKQK-----LNLIKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKIKNDKRPIIDIKVQKERLLNKLKLIIDTNWQKVINETKGIDKVKWTNRINKLIQLIKLNQPDKIDLKWLQKFKRIINIVKKNAIKKPITVKIITKNVKRF---IKRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 814

HSP 18 Score: 80.8777 bits (198), Expect = 1.672e-15
Identity = 71/254 (27.95%), Postives = 127/254 (50.00%), Query Frame = 2 
Query:   32 EENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTL-----RKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQI--LATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKI 772
            E+ L  I + S+KY+  L EL W  L+  ID  ++  +  KI  FI  V  ++Y KI+   +NWL+ II TL     R    +  K  +I+++ L  +   SN          W+ ++  +   N    I +I+  +  ++  E++KID G I+ + ++  L T + ++T+            +V IKT ++ +  L +  WE  ++YIDE+ R E+ + +N  I+ ++N  YD +DV  V++  QI     K+
Sbjct: 2723 EDKLNFIIDMSHKYLVILNELRWNELIDSIDGFNKRSIFVKINKFIHYVNTRKYHKINRKFLNWLSQIIITLQNTKIRLTGSMKQKRIEIAKKVL--VNLPSN----------WKEVIVKLPNINVDLWIKRIQLLLPMIELNEFDKIDFGWIDELQKLVDLITEESIKTVEKPKVN------EVTIKTVKENVNSLKQWNWETYLKYIDEDKREEVQRNVNNVITIIENVEYDKLDVKSVDWFNQIFEVFNKM 2958

HSP 19 Score: 79.7221 bits (195), Expect = 2.935e-15
Identity = 86/403 (21.34%), Postives = 201/403 (49.88%), Query Frame = 2 
Query:   71 YINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKIDTGLINFIAQILATLKQVRT------IHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTS-----RRXXXXXXXXXXLPGNWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKIDFDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKI 1246
            ++ +L++  W  L+K++  + +  V K +     +++ +++EKI+++L+  +  ++  L+++ ++H K     + N+        + +  +   +W  ++  ++  +R + I++  K    ++ K Y +ID   +N++A+    + + R       IH K +K          K    Y+ +L    W+ L+  ++E  +  I K+I      +K ++++ I+VDL+     I   L+KI+ +K+        ++ ++KKI++ +  NW+ ++  ++GVD   W  R  K++ M+ +    KIDF W+ + ++I+ LI  +           +  +  +K  V +L +W W   +  IDE +R+ V+K++  ++ +V+  +F++++  L      IF+   KI
Sbjct:  279 HVEWLKKWKWMELLKFVSGNYKERVTKTVNELFVIIKRQEFEKINLELVKNVKFVMRLLKRLWRIHIKYGSRKKANMI-------RRLKLILSIDWRRIINELEGIDRNQWIERFNKLKNLIQLKRYNEID---VNWLAEFKPIIDKFRGKIKINYIHQKRQK----------KKIRLYLRLLKRWNWKNLVVDLNEKEKENIAKEIELMKRIIKEEHFNEINVDLIKRFKVIFKILRKIKQSKYPKGNVKVTKEILLKKIKLIIDSNWLNIINGLEGVDKVKWTTRFNKIIQMIRNNNIDKIDFKWLLKFKRILKLIHRKSHPR-------KQKLNLIKKFVNRLIKWNWPDLIKNIDEKDRENVKKEIELVLKLVKENKFKEIDENLYKRIRVIFKTIRKI 654

HSP 20 Score: 72.7886 bits (177), Expect = 4.429e-13
Identity = 106/453 (23.40%), Postives = 209/453 (46.14%), Query Frame = 2 
Query:   74 INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEKI----DTGLINFIAQI-LATLKQVRTIHXXXXXX-XXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKIRVTKFTSRRXXXXXXXXXXLPG---------NWVELLRRIKGVDHDLWIGRIKKLLSMMESKEYQKID---FDWXXXXXXXXXXXXXXXXXXXXXXXXNEGSIAKLKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQLVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRMRMKRSAGLNYMAESQAK 1378
            IN L+  NWE  ++YIDE  R  +   I   I +++N + +K+D D I+W   I +  +K++                     N Y+   N   W          N  EV +KI+K   +  +K + KI        + F  +I +  L   + IH K+ +    +    I+    K +  L +  W+ LM+Y+ +N + + + KI   IS +KNK+YDNID DL+ +I  I T L+ +R  K  ++  D   KI +K             W++++  I+G +      +IK+ +++++++EY KID    +W    + I ++   ++   + +   N   I K++ N+       W+  L  ++  +       +  ++S+V+S++++K++ + ++  ++I Q+ +         +V   + KT +K   +NS  +    + L Y+ E+  K
Sbjct: 3037 INSLKLWNWENFLQYIDEDKREPIQTNINKIINVIENGESDKLDPDTIDWFIQIFEEFKKINYA-----------------IENGYVYDPNTRTW--------TKNTVEVENKIRKKRCF--SKGHRKIRKFRPHNNLKFHNKIHINNLFHWKKIHQKHHRKIVSKERKRIVHKVIKRVNWLEKWNWKNLMKYVVDNHKRDTLVKIKYIISLIKNKDYDNIDEDLLKWISTIKTKLKIVRKIKKETKNIDENLKIVMKKSHKFVDILNQLRWIDIIDEIEGDNKPEIKEKIKRFITLVQNREYDKIDTEFLNW--LFEIIENIKKTKQKLTETDNTKNVKVIEKIQINLPS----NWKELLQNVEGIDVNLWTARIENLLSLVQSKEYQKIDFEWIDELQKIVQLISTQVTESKEKFVLPESTKTKIKE-NINSLKQWNWESLLKYIEETNRK 3455

HSP 21 Score: 65.855 bits (159), Expect = 6.544e-11
Identity = 69/257 (26.85%), Postives = 116/257 (45.14%), Query Frame = 2 
Query:   11 IKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENLKIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEYEK--IDTGLINFIAQ-ILATLKQVRTIHXXXXXXXXXXVDVIIKTSEKYIVMLNELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQIITTLQKI 772
            ++  KV E  +KT+ E     +N L++ NWE  +KYIDE  R EV + +   IT+++N +Y+K+DV  ++W   I +                    K+   T+N Y+               DE  +  V     K +V V+N   ++  I   L++  A+ ++  +     I  K  + +     V I+T    I  L    WE  +QYIDE+ R  I   IN+ I+ ++N   D +D D +++  QI    +KI
Sbjct: 2881 VEKPKVNEVTIKTVKEN----VNSLKQWNWETYLKYIDEDKREEVQRNVNNVITIIENVEYDKLDVKSVDWFNQIFEVFN-----------------KMYYATTNGYV--------------YDEKTKSWV-----KTVVEVENNTRKRRCILLNLLHGAAKKLIPHISIGLNIGGKPRRPRPNYNQVSIQTIRTNINSLKLWNWENFLQYIDEDKREPIQTNINKIINVIENGESDKLDPDTIDWFIQIFEEFKKI 3097

HSP 22 Score: 50.447 bits (119), Expect = 3.672e-6
Identity = 29/90 (32.22%), Postives = 52/90 (57.78%), Query Frame = 2 
Query:    5 VHIKNEKVTEENLKTITETSNKY-----INFLRELNWEVLVKYIDESSRPEVIKKITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVS 259
            V + + +VTE   K +   S K      IN L++ NWE L+KYI+E++R +V   +   I +V+N++++KID   + W   I++   K++
Sbjct: 3406 VQLISTQVTESKEKFVLPESTKTKIKENINSLKQWNWESLLKYIEETNRKQVETDVKKMIVIVENEEFDKIDSQFVQWFNQIVELFNKIN 3495
The following BLAST results are available for this feature:
BLAST of SMED30014784 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A1S6KMJ12.886e-8444.15Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
A0A1S6KMJ81.632e-1521.79Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
A0A1S6KMJ47.277e-1023.99Uncharacterized protein (Fragment) OS=Schmidtea me... [more]
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BLAST of SMED30014784 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014784 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000008843.15.048e-11999.63SMESG000008843.1[more]
SMESG000008835.17.482e-12855.19SMESG000008835.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014784 ID=SMED30014784|Name=SMED30014784|organism=Schmidtea mediterranea sexual|type=transcript|length=1623bp
GCAGGTTCACATTAAAAATGAAAAAGTTACCGAAGAAAATTTGAAAACAA
TAACTGAGACATCAAATAAATACATCAATTTCTTGAGAGAACTGAATTGG
GAAGTACTAGTGAAATATATAGATGAAAGCAGTCGGCCAGAAGTGATCAA
GAAAATAACAACTTTTATCACTCTTGTTCAAAATAAACAATATGAAAAGA
TAGATGTTGATCTAATAAATTGGTTAACGACAATTATAGATACATTGAGA
AAAGTAAGCCAGGTTCATAATAAAAACGAAAAAATAAGTCAAGAAAATTT
GAAAATAATAACTGAAACTTCAAACAAATATATTGACGCATTAAACGAAT
ATGAATGGGAAATACTAATGAAATACATTGATGAAAGCAACCGACCGGAA
GTCATCGACAAAATTAAAAAGTTCATTGTCTATGTTAAAAATAAAGAATA
TGAAAAAATTGATACTGGTTTAATAAATTTCATTGCTCAAATTTTAGCAA
CGTTAAAACAAGTAAGAACCATTCACATTAAGAATGAGAAAATTAAAAAA
GAAAATGTTGATGTTATTATCAAAACTTCCGAGAAATACATAGTTATGCT
GAATGAGCTGAAATGGGAAGAATTGATGCAGTATATCGACGAAAACAGTC
GACCAGAAATCATTAAAAAGATCAACGAGTTCATTTCTCATGTAAAAAAC
AAAAATTATGACAATATTGATGTCGATTTAGTTAACTATATTGGTCAAAT
CATCACGACATTGCAAAAGATAAGAGTAACGAAATTCACATCGAGACGAA
AAGACATCATTAAGAAAATACAAATCAAGTTGCCCGGAAATTGGGTAGAA
CTTCTAAGGCGAATTAAAGGAGTCGATCATGATCTATGGATCGGACGTAT
TAAAAAGCTTTTGTCTATGATGGAATCTAAGGAATATCAAAAAATTGATT
TTGATTGGATTGAAGAATTGCAAAAAATTATTAGCTTAATATCCGAAGAA
GAAATCAAATTAAAGAAAGAAGTTATTCTCAATGAAGGTTCAATTGCTAA
ATTGAAAAGTAATGTCAAACAATTGCAAGAATGGAAATGGGAATCCTATT
TAGCATATATTGATGAAAGTAATCGCCAACAGGTTGAAAAAGATGTTAGA
GAAATTATATCAATTGTTGAAAGTGAACAATTTGAGAAACTAGAAATCCA
ACTCGTAAATTGGTTTGAACAAATTTTCCAAATATTTACAAAAATTAATG
ACGCGATTACCAACGGATATGTTTTTGATTCAGCAAAAAAAACATGGGTA
AAAAACATTGTTGTTAATAGCAGAATGAGAATGAAAAGATCTGCTGGATT
AAATTATATGGCTGAATCTCAAGCAAAAATGAATGTCGAATTACTAAACC
AATCGATTGAAGCACTCAGAAAAGCACTAATGTACAATATGATGATTTTG
AGCAATAATGGCCAAGAATCTGTCAGGAAAACCGACCAAAATGATAATCA
ATCTCATCTGTGTCCATTTATTGACAAAGTTAATAACAAATTAACAACAA
AATTGACAAATTGTGAAATGAAGCAAAGATTTATATGCAAAAGACAATTA
AATTAATAAAACATCTTTGAAAC
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protein sequence of SMED30014784-orf-1

>SMED30014784-orf-1 ID=SMED30014784-orf-1|Name=SMED30014784-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=535bp
QVHIKNEKVTEENLKTITETSNKYINFLRELNWEVLVKYIDESSRPEVIK
KITTFITLVQNKQYEKIDVDLINWLTTIIDTLRKVSQVHNKNEKISQENL
KIITETSNKYIDALNEYEWEILMKYIDESNRPEVIDKIKKFIVYVKNKEY
EKIDTGLINFIAQILATLKQVRTIHIKNEKIKKENVDVIIKTSEKYIVML
NELKWEELMQYIDENSRPEIIKKINEFISHVKNKNYDNIDVDLVNYIGQI
ITTLQKIRVTKFTSRRKDIIKKIQIKLPGNWVELLRRIKGVDHDLWIGRI
KKLLSMMESKEYQKIDFDWIEELQKIISLISEEEIKLKKEVILNEGSIAK
LKSNVKQLQEWKWESYLAYIDESNRQQVEKDVREIISIVESEQFEKLEIQ
LVNWFEQIFQIFTKINDAITNGYVFDSAKKTWVKNIVVNSRMRMKRSAGL
NYMAESQAKMNVELLNQSIEALRKALMYNMMILSNNGQESVRKTDQNDNQ
SHLCPFIDKVNNKLTTKLTNCEMKQRFICKRQLN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 320..340