SMED30014769

Overview
NameSMED30014769
Smed IDSMED30014769
Length (bp)1668
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014769 (SMED30014769) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014769 (SMED30014769) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014769 (SMED30014769) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014769 (SMED30014769) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014769

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30014769h1SMcG0008965 Contig922GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30014769h1SMcG0008965 Contig50110uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014769h1SMcG0008965 Contig50110newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014769h1SMcG0008965 Contig8857uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014769h1SMcG0008965 Contig8857newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30014769h1SMcG0008965 dd_Smed_v4_725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014769h1SMcG0008965 dd_Smed_v4_725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30014769h1SMcG0008965 dd_Smed_v4_725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30014769h1SMcG0008965 dd_Smed_v4_725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014769h1SMcG0008965 dd_Smed_v4_725_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014769h1SMcG0008965 Contig29403newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014769h1SMcG0008965 Contig29403uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
body wall musculatureSMED30014769h1SMcG0008965 dd_Smed_v4_725_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30014769h1SMcG0008965 dd_Smed_v6_725_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014769 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000049:86770..88436 -8112
Homology
[Close]
BLAST of SMED30014769 vs. Ensembl Human
Match: HADH (hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4799])

HSP 1 Score: 164.851 bits (416), Expect = 1.750e-47
Identity = 78/137 (56.93%), Postives = 99/137 (72.26%), Query Frame = -2 
Query:  129 APVASHTHTQIYIIYNWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYS 539
            A +A    T +     +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPL  P   L+K+VA    G+K+G+GFY Y 
Sbjct:   40 AGIAQVIKTPMTSQKTFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKYK 176
[Close]
BLAST of SMED30014769 vs. Ensembl Human
Match: HADH (hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4799])

HSP 1 Score: 167.548 bits (423), Expect = 2.859e-47
Identity = 75/121 (61.98%), Postives = 94/121 (77.69%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPL  P   L+K+VA    G+K+G+GFY Y
Sbjct:  146 TFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKY 266

HSP 2 Score: 79.7221 bits (195), Expect = 6.589e-16
Identity = 40/61 (65.57%), Postives = 42/61 (68.85%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I  +A AT RQDRF GLHFFNPVPVM LVEV+ T  TS  T E
Sbjct:   88 DKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFE 148
[Close]
BLAST of SMED30014769 vs. Ensembl Human
Match: HADH (hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4799])

HSP 1 Score: 168.318 bits (425), Expect = 5.300e-47
Identity = 75/121 (61.98%), Postives = 94/121 (77.69%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPL  P   L+K+VA    G+K+G+GFY Y
Sbjct:  193 TFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKY 313

HSP 2 Score: 80.1073 bits (196), Expect = 7.958e-16
Identity = 40/61 (65.57%), Postives = 42/61 (68.85%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I  +A AT RQDRF GLHFFNPVPVM LVEV+ T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFE 195
[Close]
BLAST of SMED30014769 vs. Ensembl Human
Match: HADH (hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4799])

HSP 1 Score: 166.777 bits (421), Expect = 2.129e-46
Identity = 75/121 (61.98%), Postives = 94/121 (77.69%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPL  P   L+K+VA    G+K+G+GFY Y
Sbjct:  197 TFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKY 317

HSP 2 Score: 79.7221 bits (195), Expect = 1.175e-15
Identity = 40/61 (65.57%), Postives = 42/61 (68.85%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I  +A AT RQDRF GLHFFNPVPVM LVEV+ T  TS  T E
Sbjct:  139 DKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFE 199
[Close]
BLAST of SMED30014769 vs. Ensembl Human
Match: HADH (hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4799])

HSP 1 Score: 162.925 bits (411), Expect = 5.388e-45
Identity = 75/124 (60.48%), Postives = 94/124 (75.81%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLER---GEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ER   G+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPL  P   L+K+VA    G+K+G+GFY Y
Sbjct:  193 TFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERVSDGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKY 316

HSP 2 Score: 80.1073 bits (196), Expect = 8.324e-16
Identity = 40/61 (65.57%), Postives = 42/61 (68.85%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I  +A AT RQDRF GLHFFNPVPVM LVEV+ T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFE 195
[Close]
BLAST of SMED30014769 vs. Ensembl Celegans
Match: B0272.3 (Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 [Source:UniProtKB/Swiss-Prot;Acc:P41938])

HSP 1 Score: 170.629 bits (431), Expect = 2.447e-48
Identity = 80/134 (59.70%), Postives = 98/134 (73.13%), Query Frame = -2 
Query:  132 VASHTHTQIYIIYNWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 533
            V  HT T       +  L+ + K+VGKT V C+DTPGFIVNRLLVPY+ EALR+ ERG+A+ EDIDVAM+LGAGYPMGP  L+DYVGLDT KFI+DGWH ++P    F P  LL+ +V  G  G+KSG+GFY Y
Sbjct:  178 VVRHTETSDA---TFNQLVDYGKTVGKTTVACKDTPGFIVNRLLVPYMFEALRLYERGDASMEDIDVAMKLGAGYPMGPFELSDYVGLDTCKFIMDGWHAQYPEEVAFTPSPLLNSLVDSGKNGRKSGEGFYKY 308

HSP 2 Score: 61.2326 bits (147), Expect = 4.966e-10
Identity = 31/58 (53.45%), Postives = 39/58 (67.24%), Query Frame = -1 
Query:  766 AAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 939
            AA P+  I  +NTSSL +AD+ +    + RFGGLHFFNPVP+M L+EVV    TS  T
Sbjct:  132 AAKPTTLI-TTNTSSLRLADIGLNLKDKSRFGGLHFFNPVPMMKLLEVVRHTETSDAT 188
[Close]
BLAST of SMED30014769 vs. Ensembl Celegans
Match: F54C8.1 (Probable 3-hydroxyacyl-CoA dehydrogenase F54C8.1 [Source:UniProtKB/Swiss-Prot;Acc:P34439])

HSP 1 Score: 160.614 bits (405), Expect = 7.598e-45
Identity = 72/121 (59.50%), Postives = 90/121 (74.38%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +  L+ F  +VGKT V C+D+PGFIVNRLL+PY  EA RM ERG+A+  DID AM+LGAG+PMGP  LADY+GLDT KF++DGW  K+P   LF    L+DK+VA G LG+K+G GFY Y
Sbjct:  176 TYATLIKFGTAVGKTTVACKDSPGFIVNRLLIPYFFEAARMYERGDASMTDIDEAMKLGAGHPMGPFELADYIGLDTVKFVMDGWAAKYPEVQLFEASPLVDKLVAEGKLGRKTGDGFYSY 296

HSP 2 Score: 61.6178 bits (148), Expect = 3.052e-10
Identity = 27/51 (52.94%), Postives = 36/51 (70.59%), Query Frame = -1 
Query:  766 IFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 918
            I  +NTSS  + D+A     + RFGGLHFFNPVPVM L+EV+ + +TS +T
Sbjct:  126 ILTTNTSSFLLEDVAKGLQDKTRFGGLHFFNPVPVMKLLEVIRSDDTSDET 176
[Close]
BLAST of SMED30014769 vs. Ensembl Celegans
Match: hacd-1 (Hydroxy-Acyl-CoA Dehydrogenase [Source:UniProtKB/TrEMBL;Acc:O44608])

HSP 1 Score: 141.354 bits (355), Expect = 9.500e-38
Identity = 65/117 (55.56%), Postives = 82/117 (70.09%), Query Frame = -2 
Query:  132 LLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 482
            L  F K + K  V  +DTPGFIVNRLL+PY+ +++RMLERG+AT EDID AMR G  YPMGP+ L DYVGLD  +  L  + E  P +  F PI L+DK+VA G LG+K+ +GFY Y
Sbjct:  183 LFNFCKEIKKLPVAAKDTPGFIVNRLLIPYLMDSIRMLERGDATKEDIDTAMRFGTSYPMGPIELCDYVGLDVLQSTLKIFRETIPGDARFAPIPLMDKLVAEGKLGRKTKQGFYTY 299

HSP 2 Score: 67.3958 bits (163), Expect = 4.112e-12
Identity = 31/56 (55.36%), Postives = 36/56 (64.29%), Query Frame = -1 
Query:  763 PSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTT 930
            P  C+  +NTSSL ++ M         F GLHFFNPVPVM LVEVVST  TS +TT
Sbjct:  125 PQNCMLITNTSSLKLSQMLPVIQNPALFAGLHFFNPVPVMKLVEVVSTDETSPETT 180
[Close]
BLAST of SMED30014769 vs. Ensembl Celegans
Match: ech-1.2 (Enoyl-CoA Hydratase [Source:UniProtKB/TrEMBL;Acc:Q9GYT0])

HSP 1 Score: 59.3066 bits (142), Expect = 5.892e-9
Identity = 39/114 (34.21%), Postives = 57/114 (50.00%), Query Frame = -2 
Query:  132 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIG-----LLDKMVAGGNLGQKSGKGFYDY 458
            GK  V  +D PGF V R L P   E +R+L+ G    E   +  +   G+P+G   LAD VGLD  + +      +F    L   +G     LL ++V  G+ G+K+GKG + Y
Sbjct:  550 GKLVVVVKDCPGFFVVRCLGPMTAEIMRLLQEGVDPAELDKLTTKF--GFPVGAATLADEVGLDVAEHV-----AQFLGKALGPRLGGGSADLLSELVKAGHKGRKTGKGIFVY 656

HSP 2 Score: 54.6842 bits (130), Expect = 1.481e-7
Identity = 24/51 (47.06%), Postives = 37/51 (72.55%), Query Frame = -1 
Query:  766 IFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 918
            I ASNTS+L I D+A A++R D+  G+H+F+PV  M L+E+++   TS +T
Sbjct:  488 IIASNTSALPIKDIAAASSRPDKVIGMHYFSPVEKMQLLEIITHDGTSKET 538
[Close]
BLAST of SMED30014769 vs. Ensembl Celegans
Match: ech-1.2 (Enoyl-CoA Hydratase [Source:UniProtKB/TrEMBL;Acc:Q9GYT0])

HSP 1 Score: 58.9214 bits (141), Expect = 6.695e-9
Identity = 39/114 (34.21%), Postives = 57/114 (50.00%), Query Frame = -2 
Query:  132 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIG-----LLDKMVAGGNLGQKSGKGFYDY 458
            GK  V  +D PGF V R L P   E +R+L+ G    E   +  +   G+P+G   LAD VGLD  + +      +F    L   +G     LL ++V  G+ G+K+GKG + Y
Sbjct:  550 GKLVVVVKDCPGFFVVRCLGPMTAEIMRLLQEGVDPAELDKLTTKF--GFPVGAATLADEVGLDVAEHV-----AQFLGKALGPRLGGGSADLLSELVKAGHKGRKTGKGIFVY 656

HSP 2 Score: 54.6842 bits (130), Expect = 1.630e-7
Identity = 24/51 (47.06%), Postives = 37/51 (72.55%), Query Frame = -1 
Query:  766 IFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 918
            I ASNTS+L I D+A A++R D+  G+H+F+PV  M L+E+++   TS +T
Sbjct:  488 IIASNTSALPIKDIAAASSRPDKVIGMHYFSPVEKMQLLEIITHDGTSKET 538
[Close]
BLAST of SMED30014769 vs. Ensembl Fly
Match: Mtpalpha (gene:FBgn0028479 transcript:FBtr0079857)

HSP 1 Score: 57.7658 bits (138), Expect = 1.923e-8
Identity = 36/114 (31.58%), Postives = 58/114 (50.88%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +   D PGF   R+L   + EA+R+L+ G    +D+D   +   G+P+G   LAD VG+D    I     + F        + +++ +V  G LG+KSGKG + Y  + +
Sbjct:  506 GKVVITVGDGPGFYTTRILSTMLSEAIRLLQEG-VDPKDLDQYTK-KFGFPVGAATLADEVGIDVGSHIAVDLAKAFGERFSGGNLEVMNDLVLAGFLGRKSGKGIFLYDGQKR 617

HSP 2 Score: 54.6842 bits (130), Expect = 1.912e-7
Identity = 22/59 (37.29%), Postives = 39/59 (66.10%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            +A  P  C+ A+NTS++ I  +A  ++R ++  G+H+F+PV  M L+E+++   TS DT
Sbjct:  436 EAVVPEHCVIATNTSAIPITKIAAGSSRPEKVVGMHYFSPVDKMQLLEIITHPGTSKDT 494
[Close]
BLAST of SMED30014769 vs. Ensembl Fly
Match: Mtpalpha (gene:FBgn0028479 transcript:FBtr0079858)

HSP 1 Score: 57.7658 bits (138), Expect = 2.122e-8
Identity = 36/114 (31.58%), Postives = 58/114 (50.88%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +   D PGF   R+L   + EA+R+L+ G    +D+D   +   G+P+G   LAD VG+D    I     + F        + +++ +V  G LG+KSGKG + Y  + +
Sbjct:  545 GKVVITVGDGPGFYTTRILSTMLSEAIRLLQEG-VDPKDLDQYTK-KFGFPVGAATLADEVGIDVGSHIAVDLAKAFGERFSGGNLEVMNDLVLAGFLGRKSGKGIFLYDGQKR 656

HSP 2 Score: 54.6842 bits (130), Expect = 1.883e-7
Identity = 22/59 (37.29%), Postives = 39/59 (66.10%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            +A  P  C+ A+NTS++ I  +A  ++R ++  G+H+F+PV  M L+E+++   TS DT
Sbjct:  475 EAVVPEHCVIATNTSAIPITKIAAGSSRPEKVVGMHYFSPVDKMQLLEIITHPGTSKDT 533
[Close]
BLAST of SMED30014769 vs. Ensembl Zebrafish
Match: hadh (hydroxyacyl-CoA dehydrogenase [Source:ZFIN;Acc:ZDB-GENE-040801-261])

HSP 1 Score: 174.481 bits (441), Expect = 1.277e-49
Identity = 79/121 (65.29%), Postives = 98/121 (80.99%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             + ALL F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG  + EDIDVAM+LGAGYPMGP  L DYVGLDT+KFI+DGWH K P+NPLF P  LL+K+V+ G LG+K+G+GFY +
Sbjct:  188 TFDALLEFSKALGKHPVSCKDTPGFIVNRLLVPYMLEAVRLHERGHGSKEDIDVAMKLGAGYPMGPFELLDYVGLDTSKFIIDGWHAKDPDNPLFAPSPLLNKLVSEGKLGKKTGEGFYKH 308

HSP 2 Score: 82.0333 bits (201), Expect = 1.010e-16
Identity = 40/59 (67.80%), Postives = 43/59 (72.88%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D  AP   IFASNTSSL IAD+A  T R DRFGGLHFFNPVP+M LVEV+ T  TS  T
Sbjct:  130 DKVAPEHTIFASNTSSLPIADIASCTARLDRFGGLHFFNPVPMMKLVEVIKTPATSQQT 188
[Close]
BLAST of SMED30014769 vs. Ensembl Zebrafish
Match: hadhaa (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha a [Source:ZFIN;Acc:ZDB-GENE-031222-5])

HSP 1 Score: 64.6994 bits (156), Expect = 2.116e-10
Identity = 41/115 (35.65%), Postives = 60/115 (52.17%), Query Frame = -2 
Query:  114 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 458
            GK  V   D PGF   R L P + EA+R+L+ G    + +D ++  G G+P+G   LAD VG+D    + +   + F        +  L  MV  G  G+KSGKG Y Y +K+K+
Sbjct:  531 GKLIVVVGDGPGFYTTRCLAPMLAEAVRILQEGVGP-KKLD-SLTTGFGFPVGLATLADEVGIDVAAHVAEDLGKAFGARFGGGNVDFLKTMVQKGFKGRKSGKGCYVYGAKSKE 643

HSP 2 Score: 56.9954 bits (136), Expect = 4.979e-8
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFA+NTS+L I D+A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  461 EAVIPPHCIFATNTSALPIKDIAAASKRPEKVIGMHYFSPVDKMQLLEII 510
[Close]
BLAST of SMED30014769 vs. Ensembl Zebrafish
Match: hadhab (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha b [Source:ZFIN;Acc:ZDB-GENE-041111-204])

HSP 1 Score: 62.003 bits (149), Expect = 1.225e-9
Identity = 39/115 (33.91%), Postives = 58/115 (50.43%), Query Frame = -2 
Query:  114 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 458
            GK  +   D PGF   R L P + EA+R+L+ G    + +D ++  G G+P+G   LAD VG+D    + +   + F +      +  L  MV  G  G+KSGKG Y Y    K+
Sbjct:  533 GKVIIVVGDGPGFYTTRCLAPMLAEAVRILQEG-TDPKKLD-SLTTGFGFPVGAATLADEVGIDVAAHVAEDLGKAFGSRFGGGNVEFLKSMVEKGFKGRKSGKGCYVYQPGLKR 645

HSP 2 Score: 56.9954 bits (136), Expect = 4.652e-8
Identity = 23/50 (46.00%), Postives = 35/50 (70.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFA+NTS+L I D+A  + R D+  G+H+F+PV  M L+E++
Sbjct:  463 EAVIPPHCIFATNTSALPIKDIAAVSKRPDKVVGMHYFSPVDKMQLLEII 512
[Close]
BLAST of SMED30014769 vs. Ensembl Zebrafish
Match: ehhadh (enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase [Source:NCBI gene;Acc:100000859])

HSP 1 Score: 58.9214 bits (141), Expect = 1.228e-8
Identity = 42/131 (32.06%), Postives = 65/131 (49.62%), Query Frame = -2 
Query:  132 LAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKF----------PNNPLFNPIG-----LLDKMVAGGNLGQKSGKGFYDY 479
            ++  K +GK +V   + PGF+ NR+L+PY+ +A  +LE G AT + ID A+    G+ MG   ++D  GLD       GW  +           P +P     G     + D +   G  GQK+G+G+Y Y
Sbjct:  455 MSLGKRMGKVSVAVGNCPGFVGNRMLMPYLEQATFLLEEG-ATPQQIDKALE-DFGFAMGVFRMSDLAGLDV------GWRVRKESGLTGPDVDPKDPPRRRQGRKYCPIPDMVCQQGRFGQKTGRGWYMY 577
[Close]
BLAST of SMED30014769 vs. Ensembl Xenopus
Match: vps41 (VPS41, HOPS complex subunit [Source:Xenbase;Acc:XB-GENE-999710])

HSP 1 Score: 178.718 bits (452), Expect = 6.617e-51
Identity = 79/121 (65.29%), Postives = 99/121 (81.82%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GKT V C+DTPGFIVNRLLVPY+ E++R+ ERG A+ EDIDVAM+LGAGYPMGP  L DYVGLDT+K+I+DGWH   PNNPLF P  LLDK+VA   LG+K+G+GFY Y
Sbjct:  192 TFESLMDFSKALGKTPVSCKDTPGFIVNRLLVPYLMESVRLYERGHASKEDIDVAMKLGAGYPMGPFELLDYVGLDTSKYIIDGWHALEPNNPLFAPSELLDKLVAEKKLGKKTGEGFYKY 312

HSP 2 Score: 84.3445 bits (207), Expect = 2.639e-17
Identity = 40/61 (65.57%), Postives = 45/61 (73.77%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  AP   IFASNTSSL I D+A +T RQDRFGGLHFFNPVP+M L+EV+ T  TS  T E
Sbjct:  134 DKFAPEHTIFASNTSSLPITDIANSTTRQDRFGGLHFFNPVPMMKLIEVIKTPMTSQTTFE 194
[Close]
BLAST of SMED30014769 vs. Ensembl Xenopus
Match: vps41 (VPS41, HOPS complex subunit [Source:Xenbase;Acc:XB-GENE-999710])

HSP 1 Score: 178.718 bits (452), Expect = 6.982e-51
Identity = 79/121 (65.29%), Postives = 99/121 (81.82%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GKT V C+DTPGFIVNRLLVPY+ E++R+ ERG A+ EDIDVAM+LGAGYPMGP  L DYVGLDT+K+I+DGWH   PNNPLF P  LLDK+VA   LG+K+G+GFY Y
Sbjct:  194 TFESLMDFSKALGKTPVSCKDTPGFIVNRLLVPYLMESVRLYERGHASKEDIDVAMKLGAGYPMGPFELLDYVGLDTSKYIIDGWHALEPNNPLFAPSELLDKLVAEKKLGKKTGEGFYKY 314

HSP 2 Score: 84.7297 bits (208), Expect = 2.446e-17
Identity = 40/61 (65.57%), Postives = 45/61 (73.77%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  AP   IFASNTSSL I D+A +T RQDRFGGLHFFNPVP+M L+EV+ T  TS  T E
Sbjct:  136 DKFAPEHTIFASNTSSLPITDIANSTTRQDRFGGLHFFNPVPMMKLIEVIKTPMTSQTTFE 196
[Close]
BLAST of SMED30014769 vs. Ensembl Xenopus
Match: hadha (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:394832])

HSP 1 Score: 71.2478 bits (173), Expect = 2.166e-12
Identity = 44/114 (38.60%), Postives = 60/114 (52.63%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +  +D PGF   R L P + E +R+L+ G    + +D A+  G G+P+G   LAD VG+D    + +   + F        I LL  MVA G LG+KSGKGFY Y    K
Sbjct:  534 GKVIIVVKDGPGFYTTRCLGPMMAEVVRVLQEG-VDPKKLD-ALSTGFGFPVGASTLADEVGIDVAAHVAEDLGKAFGERFGGGNIELLKAMVAKGFLGRKSGKGFYIYQQGVK 645

HSP 2 Score: 56.6102 bits (135), Expect = 9.610e-8
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFASNTS+L I+ +A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  464 EAVVPPHCIFASNTSALPISQIAAASKRPEKVIGMHYFSPVDKMQLLEII 513
[Close]
BLAST of SMED30014769 vs. Ensembl Xenopus
Match: hadha (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:394832])

HSP 1 Score: 71.2478 bits (173), Expect = 2.290e-12
Identity = 44/114 (38.60%), Postives = 60/114 (52.63%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +  +D PGF   R L P + E +R+L+ G    + +D A+  G G+P+G   LAD VG+D    + +   + F        I LL  MVA G LG+KSGKGFY Y    K
Sbjct:  539 GKVIIVVKDGPGFYTTRCLGPMMAEVVRVLQEG-VDPKKLD-ALSTGFGFPVGASTLADEVGIDVAAHVAEDLGKAFGERFGGGNIELLKAMVAKGFLGRKSGKGFYIYQQGVK 650

HSP 2 Score: 56.6102 bits (135), Expect = 9.551e-8
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFASNTS+L I+ +A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  469 EAVVPPHCIFASNTSALPISQIAAASKRPEKVIGMHYFSPVDKMQLLEII 518
[Close]
BLAST of SMED30014769 vs. Ensembl Xenopus
Match: hadha (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:394832])

HSP 1 Score: 71.2478 bits (173), Expect = 2.315e-12
Identity = 44/114 (38.60%), Postives = 60/114 (52.63%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +  +D PGF   R L P + E +R+L+ G    + +D A+  G G+P+G   LAD VG+D    + +   + F        I LL  MVA G LG+KSGKGFY Y    K
Sbjct:  508 GKVIIVVKDGPGFYTTRCLGPMMAEVVRVLQEG-VDPKKLD-ALSTGFGFPVGASTLADEVGIDVAAHVAEDLGKAFGERFGGGNIELLKAMVAKGFLGRKSGKGFYIYQQGVK 619

HSP 2 Score: 56.6102 bits (135), Expect = 8.922e-8
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFASNTS+L I+ +A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  438 EAVVPPHCIFASNTSALPISQIAAASKRPEKVIGMHYFSPVDKMQLLEII 487
[Close]
BLAST of SMED30014769 vs. Ensembl Mouse
Match: Hadh (hydroxyacyl-Coenzyme A dehydrogenase [Source:MGI Symbol;Acc:MGI:96009])

HSP 1 Score: 172.94 bits (437), Expect = 6.169e-49
Identity = 77/121 (63.64%), Postives = 96/121 (79.34%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFILDGWHE  P NPLF P   ++ +VA   LG+K+G+GFY Y
Sbjct:  193 TFESLVDFCKTLGKHPVSCKDTPGFIVNRLLVPYLIEAVRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEMEPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKY 313

HSP 2 Score: 79.337 bits (194), Expect = 1.104e-15
Identity = 39/61 (63.93%), Postives = 43/61 (70.49%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I ++A AT RQDRF GLHFFNPVP+M LVEV+ T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFE 195
[Close]
BLAST of SMED30014769 vs. Ensembl Mouse
Match: Hadha (hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit [Source:MGI Symbol;Acc:MGI:2135593])

HSP 1 Score: 74.3294 bits (181), Expect = 2.004e-13
Identity = 42/114 (36.84%), Postives = 63/114 (55.26%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +  +D PGF   R L P + E +R+L+ G    + +D A+  G G+P+G   LAD VG+D  + + +   + F        + LL +MV+ G LG+KSGKGFY Y   +K
Sbjct:  533 GKVIIVVKDGPGFYTTRCLAPMMSEVMRILQEG-VDPKKLD-ALTTGFGFPVGAATLADEVGVDVAQHVAEDLGKAFGERFGGGSVELLKQMVSKGFLGRKSGKGFYIYQEGSK 644

HSP 2 Score: 52.7582 bits (125), Expect = 1.136e-6
Identity = 21/50 (42.00%), Postives = 34/50 (68.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            ++  P  CIFASNTS+L I  +A  + R ++  G+H+F+PV  M L+E++
Sbjct:  463 ESVTPEHCIFASNTSALPINQIAAVSKRPEKVIGMHYFSPVDKMQLLEII 512
[Close]
BLAST of SMED30014769 vs. Ensembl Mouse
Match: Ehhadh (enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [Source:MGI Symbol;Acc:MGI:1277964])

HSP 1 Score: 58.9214 bits (141), Expect = 1.325e-8
Identity = 39/128 (30.47%), Postives = 62/128 (48.44%), Query Frame = -2 
Query:  132 LLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPN-----------NPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 482
            +++  K +GK  V   +  GF+ NR+L PY  +   ++E G +  ED+D  +    G+ MGP  ++D  GLD    +  G     P+           N  ++PI   D +   G  GQK+GKG+Y Y
Sbjct:  454 VMSLSKRIGKIGVVVGNCYGFVGNRMLAPYYNQGYFLIEEG-SKPEDVDGVLE-EFGFRMGPFRVSDLAGLDVGWKVRKGQGLTGPSLPPGTPTRKRGNTRYSPIA--DMLCEAGRFGQKTGKGWYQY 577
[Close]
BLAST of SMED30014769 vs. UniProt/SwissProt
Match: sp|Q9WVK7|HCDH_RAT (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Hadh PE=2 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 2.766e-48
Identity = 77/121 (63.64%), Postives = 96/121 (79.34%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFILDGWHE  P NPLF P   ++ +VA   LG+K+G+GFY Y
Sbjct:  193 TFESLVDFCKTLGKHPVSCKDTPGFIVNRLLVPYLIEAIRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEMDPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKY 313

HSP 2 Score: 79.337 bits (194), Expect = 6.847e-15
Identity = 39/61 (63.93%), Postives = 43/61 (70.49%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I ++A AT RQDRF GLHFFNPVP+M LVEV+ T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFE 195
[Close]
BLAST of SMED30014769 vs. UniProt/SwissProt
Match: sp|Q61425|HCDH_MOUSE (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Hadh PE=1 SV=2)

HSP 1 Score: 172.94 bits (437), Expect = 4.315e-48
Identity = 77/121 (63.64%), Postives = 96/121 (79.34%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFILDGWHE  P NPLF P   ++ +VA   LG+K+G+GFY Y
Sbjct:  193 TFESLVDFCKTLGKHPVSCKDTPGFIVNRLLVPYLIEAVRLHERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFILDGWHEMEPENPLFQPSPSMNNLVAQKKLGKKTGEGFYKY 313

HSP 2 Score: 79.337 bits (194), Expect = 7.722e-15
Identity = 39/61 (63.93%), Postives = 43/61 (70.49%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I ++A AT RQDRF GLHFFNPVP+M LVEV+ T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITNIANATTRQDRFAGLHFFNPVPMMKLVEVIKTPMTSQKTFE 195
[Close]
BLAST of SMED30014769 vs. UniProt/SwissProt
Match: sp|P41938|HCDH2_CAEEL (Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 OS=Caenorhabditis elegans OX=6239 GN=B0272.3 PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 3.113e-47
Identity = 80/134 (59.70%), Postives = 98/134 (73.13%), Query Frame = -2 
Query:  132 VASHTHTQIYIIYNWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 533
            V  HT T       +  L+ + K+VGKT V C+DTPGFIVNRLLVPY+ EALR+ ERG+A+ EDIDVAM+LGAGYPMGP  L+DYVGLDT KFI+DGWH ++P    F P  LL+ +V  G  G+KSG+GFY Y
Sbjct:  178 VVRHTETSDA---TFNQLVDYGKTVGKTTVACKDTPGFIVNRLLVPYMFEALRLYERGDASMEDIDVAMKLGAGYPMGPFELSDYVGLDTCKFIMDGWHAQYPEEVAFTPSPLLNSLVDSGKNGRKSGEGFYKY 308

HSP 2 Score: 61.2326 bits (147), Expect = 6.317e-9
Identity = 31/58 (53.45%), Postives = 39/58 (67.24%), Query Frame = -1 
Query:  766 AAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 939
            AA P+  I  +NTSSL +AD+ +    + RFGGLHFFNPVP+M L+EVV    TS  T
Sbjct:  132 AAKPTTLI-TTNTSSLRLADIGLNLKDKSRFGGLHFFNPVPMMKLLEVVRHTETSDAT 188
[Close]
BLAST of SMED30014769 vs. UniProt/SwissProt
Match: sp|P00348|HCDH_PIG (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Sus scrofa OX=9823 GN=HADH PE=1 SV=2)

HSP 1 Score: 169.859 bits (429), Expect = 6.328e-47
Identity = 75/121 (61.98%), Postives = 94/121 (77.69%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
              ++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPLF P   ++K+VA    G+K+G+GFY Y
Sbjct:  193 TLESLVDFSKTLGKHPVSCKDTPGFIVNRLLVPYLIEAVRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIIDGWHEMDSQNPLFQPSPAMNKLVAENKFGKKTGEGFYKY 313

HSP 2 Score: 80.4925 bits (197), Expect = 2.764e-15
Identity = 40/61 (65.57%), Postives = 42/61 (68.85%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I  +A AT RQDRF GLHFFNPVP+M LVEVV T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITSLANATTRQDRFAGLHFFNPVPLMKLVEVVKTPMTSQKTLE 195
[Close]
BLAST of SMED30014769 vs. UniProt/SwissProt
Match: sp|Q16836|HCDH_HUMAN (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=HADH PE=1 SV=3)

HSP 1 Score: 168.318 bits (425), Expect = 2.545e-46
Identity = 75/121 (61.98%), Postives = 94/121 (77.69%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             +++L+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG+A+ EDID AM+LGAGYPMGP  L DYVGLDTTKFI+DGWHE    NPL  P   L+K+VA    G+K+G+GFY Y
Sbjct:  193 TFESLVDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAIRLYERGDASKEDIDTAMKLGAGYPMGPFELLDYVGLDTTKFIVDGWHEMDAENPLHQPSPSLNKLVAENKFGKKTGEGFYKY 313

HSP 2 Score: 80.1073 bits (196), Expect = 3.822e-15
Identity = 40/61 (65.57%), Postives = 42/61 (68.85%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  A    IFASNTSSL I  +A AT RQDRF GLHFFNPVPVM LVEV+ T  TS  T E
Sbjct:  135 DKFAAEHTIFASNTSSLQITSIANATTRQDRFAGLHFFNPVPVMKLVEVIKTPMTSQKTFE 195
[Close]
BLAST of SMED30014769 vs. TrEMBL
Match: A0A267G4S8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028036g3 PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 6.678e-53
Identity = 86/126 (68.25%), Postives = 104/126 (82.54%), Query Frame = -2 
Query:  114 WQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 491
            +  LL F +S+GK+AV C+DTPGFIVNRLLVPY+ EA+RMLERG+AT EDID AMRLGAG+PMGP+ LADYVGLDTTKFI+DGWH KFPN  LF P  +L+K+V  G LG+K+G+GFY + SK  K
Sbjct:  150 FNQLLEFSRSLGKSAVPCKDTPGFIVNRLLVPYLTEAVRMLERGDATAEDIDTAMRLGAGHPMGPIELADYVGLDTTKFIMDGWHAKFPNVELFKPSDMLNKLVEEGRLGRKTGQGFYKWDSKGSK 275

HSP 2 Score: 79.7221 bits (195), Expect = 9.606e-13
Identity = 38/53 (71.70%), Postives = 44/53 (83.02%), Query Frame = -1 
Query:  787 DAAAPSGCIFASNTSSLSIADMAVAT-NRQDRFGGLHFFNPVPVMGLVEVVST 942
            D+ AP+GC+FASNTSSL IA++A A  NR+  FGGLHFFNPVPVM LVEVV T
Sbjct:   90 DSTAPAGCLFASNTSSLGIAELAEAVPNRKPTFGGLHFFNPVPVMRLVEVVRT 142
[Close]
BLAST of SMED30014769 vs. TrEMBL
Match: A0A1S3H0U7 (hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Lingula unguis OX=7574 GN=LOC106150701 PE=4 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 1.506e-52
Identity = 85/124 (68.55%), Postives = 102/124 (82.26%), Query Frame = -2 
Query:  123 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSK 494
             +++L+AF ++VGKT V C+DTPGF+VNRLLVPY+ EA+RMLERG+AT +DIDVAM+LGAGYPMGP  L+DYVGLDT KFILDGW E FP+ PLF    LLDKMV  G LG KSG+GFY Y+ K
Sbjct:  188 TFKSLVAFGQAVGKTTVECKDTPGFVVNRLLVPYMAEAIRMLERGDATAKDIDVAMKLGAGYPMGPFELSDYVGLDTMKFILDGWSENFPDQPLFKSSPLLDKMVENGKLGMKSGEGFYTYNKK 311

HSP 2 Score: 85.1149 bits (209), Expect = 2.142e-14
Identity = 41/59 (69.49%), Postives = 43/59 (72.88%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D  AP   IFASNTSSL I D+A AT R DRFGGLHFFNPVPVM LVEV+    TS DT
Sbjct:  130 DENAPQHTIFASNTSSLPIKDIAKATQRLDRFGGLHFFNPVPVMKLVEVIRIPETSDDT 188
[Close]
BLAST of SMED30014769 vs. TrEMBL
Match: A0A068FKF7 (Hydroxyacyl-CoA dehydrogenase OS=Agrotis segetum OX=47767 PE=2 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 1.576e-52
Identity = 84/124 (67.74%), Postives = 104/124 (83.87%), Query Frame = -2 
Query:  123 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSK 494
             +++++ + K+VGKT + C+DTPGF+VNRLLVPYI EA+R+ ERG+A+  DID AM+LGAGYPMGPL LADYVGLDT KFILDGWH+K+PN PLFNPI LLDK+VA G LG K+G+GFY Y  K
Sbjct:  190 TYKSMMEWGKAVGKTCITCKDTPGFVVNRLLVPYICEAIRLYERGDASARDIDTAMKLGAGYPMGPLELADYVGLDTNKFILDGWHKKYPNQPLFNPIPLLDKLVAEGKLGVKAGEGFYKYDKK 313

HSP 2 Score: 77.0258 bits (188), Expect = 1.262e-11
Identity = 37/61 (60.66%), Postives = 43/61 (70.49%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  AP+  IFASNTSSLSI ++     R+DRFGGLHFFNPVPVM L+EVV    TS  T +
Sbjct:  132 DGVAPNHTIFASNTSSLSINEICSVVKRKDRFGGLHFFNPVPVMRLLEVVRGAETSDATYK 192
[Close]
BLAST of SMED30014769 vs. TrEMBL
Match: A0A4C1TVX2 (Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Eumeta variegata OX=151549 GN=Hadh PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 1.990e-52
Identity = 82/123 (66.67%), Postives = 103/123 (83.74%), Query Frame = -2 
Query:  123 WQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSK 491
            +  ++ + K+VGKT + C+DTPGF+VNRLLVPYI EA+R+ ERG+A+  DID+AM+LGAGYPMGPL LADYVGLDT KFILDGWH+K+PN PLFNPI LL+K+V+ G LG K+G+GFY Y  K
Sbjct:  187 YNTMMEWGKAVGKTCITCKDTPGFVVNRLLVPYIAEAIRLFERGDASARDIDIAMKLGAGYPMGPLELADYVGLDTNKFILDGWHKKYPNEPLFNPIPLLNKLVSEGKLGVKTGQGFYTYDKK 309

HSP 2 Score: 79.337 bits (194), Expect = 1.965e-12
Identity = 39/59 (66.10%), Postives = 42/59 (71.19%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D  A    IFASNTSSLSI D+A A  R+DRFGGLHFFNPVPVM L+EVV    TS  T
Sbjct:  128 DDVADQHTIFASNTSSLSINDIATAIKRKDRFGGLHFFNPVPVMRLLEVVRGAETSDQT 186
[Close]
BLAST of SMED30014769 vs. TrEMBL
Match: A0A1S3HUR3 (hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Lingula unguis OX=7574 GN=LOC106158000 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 2.574e-52
Identity = 84/123 (68.29%), Postives = 102/123 (82.93%), Query Frame = -2 
Query:  123 WQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSK 491
            +++L+AF ++VGKT V C+DTPGF+VNRLLVPY+ EA+RMLERG+AT +DIDVAM+LGAGYPMGP  L+DYVGLDT KFILDGW + FP+ PLF    LLDKMV  G LG KSG+GFY Y+ K
Sbjct:  140 FKSLVAFGQAVGKTTVECKDTPGFVVNRLLVPYMAEAIRMLERGDATAKDIDVAMKLGAGYPMGPFELSDYVGLDTMKFILDGWSKNFPDQPLFKSSPLLDKMVENGKLGMKSGEGFYTYNKK 262

HSP 2 Score: 85.8853 bits (211), Expect = 5.956e-15
Identity = 41/59 (69.49%), Postives = 43/59 (72.88%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D  AP   IFASNTSSL I D+A AT R DRFGGLHFFNPVPVM LVEV+    TS DT
Sbjct:   81 DENAPQHTIFASNTSSLPIKDIAKATQRLDRFGGLHFFNPVPVMKLVEVIRIPETSDDT 139
[Close]
BLAST of SMED30014769 vs. Ensembl Cavefish
Match: hadh (hydroxyacyl-CoA dehydrogenase [Source:NCBI gene;Acc:103033308])

HSP 1 Score: 174.096 bits (440), Expect = 1.571e-49
Identity = 78/121 (64.46%), Postives = 98/121 (80.99%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             + AL+ F K++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG  + EDIDVAM+LGAGYP+GP  L DYVGLDT+KFI+DGW+E  P NPLF+P  LL+K+VA G LG+K+G+GFY Y
Sbjct:  188 TFDALIDFSKALGKHPVSCKDTPGFIVNRLLVPYLMEAVRLHERGHGSKEDIDVAMKLGAGYPLGPFELLDYVGLDTSKFIIDGWYETEPENPLFSPSPLLNKLVAEGKLGKKTGEGFYKY 308

HSP 2 Score: 81.6481 bits (200), Expect = 1.242e-16
Identity = 39/59 (66.10%), Postives = 43/59 (72.88%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D  AP   IFASNTSSL IAD+A +T R DRFGGLHFFNPVP+M LVEV+    TS  T
Sbjct:  130 DKVAPEHTIFASNTSSLPIADIASSTARLDRFGGLHFFNPVPMMKLVEVIKAPGTSQQT 188
[Close]
BLAST of SMED30014769 vs. Ensembl Cavefish
Match: hadhaa (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:103046024])

HSP 1 Score: 64.3142 bits (155), Expect = 2.116e-10
Identity = 45/118 (38.14%), Postives = 65/118 (55.08%), Query Frame = -2 
Query:  114 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLF---NPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 458
            GK  +   D PGF   R L P + EA+R+L+ G  T + +D ++    G+P+G  AL D VG+D    + +   + F   P F   NP  LL  MV  G  G+K+GKG+Y Y+SK K+
Sbjct:  533 GKLIIVVGDGPGFYTTRCLGPTLSEAVRVLQEG-VTPKKLD-SLTTSFGFPVGLTALIDEVGVDVAAHVAEDLGKAF--GPRFGGGNP-ELLKTMVQKGFKGRKTGKGWYIYASKKKE 645

HSP 2 Score: 56.6102 bits (135), Expect = 5.519e-8
Identity = 23/43 (53.49%), Postives = 33/43 (76.74%), Query Frame = -1 
Query:  793 CIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 921
            CIFASNTS+L I D+A A+ R D+  G+H+F+PV  M L+E++
Sbjct:  470 CIFASNTSALPIKDIAAASKRPDKVIGMHYFSPVDKMQLLEII 512
[Close]
BLAST of SMED30014769 vs. Ensembl Cavefish
Match: hadhaa (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:103046024])

HSP 1 Score: 64.3142 bits (155), Expect = 2.145e-10
Identity = 45/118 (38.14%), Postives = 65/118 (55.08%), Query Frame = -2 
Query:  114 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLF---NPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 458
            GK  +   D PGF   R L P + EA+R+L+ G  T + +D ++    G+P+G  AL D VG+D    + +   + F   P F   NP  LL  MV  G  G+K+GKG+Y Y+SK K+
Sbjct:  514 GKLIIVVGDGPGFYTTRCLGPTLSEAVRVLQEG-VTPKKLD-SLTTSFGFPVGLTALIDEVGVDVAAHVAEDLGKAF--GPRFGGGNP-ELLKTMVQKGFKGRKTGKGWYIYASKKKE 626

HSP 2 Score: 56.6102 bits (135), Expect = 5.754e-8
Identity = 23/43 (53.49%), Postives = 33/43 (76.74%), Query Frame = -1 
Query:  793 CIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 921
            CIFASNTS+L I D+A A+ R D+  G+H+F+PV  M L+E++
Sbjct:  451 CIFASNTSALPIKDIAAASKRPDKVIGMHYFSPVDKMQLLEII 493
[Close]
BLAST of SMED30014769 vs. Ensembl Cavefish
Match: hadhab (trifunctional enzyme subunit alpha, mitochondrial-like [Source:NCBI gene;Acc:103035446])

HSP 1 Score: 60.077 bits (144), Expect = 4.357e-9
Identity = 41/111 (36.94%), Postives = 58/111 (52.25%), Query Frame = -2 
Query:  129 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKF-PNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYS 458
            GK  +   D PGF   R L P + EA+R+L+ G    + +D ++  G G+P+G   LAD VG+D    + +   + F P     NP  LL  MV  G  G+K+GKG Y Y 
Sbjct:  534 GKIIIVVGDGPGFYTTRCLAPMLAEAVRVLQEG-VDPKKLD-SLTTGFGFPVGAATLADEVGIDVAAHVAEDLGKAFGPRLGGGNP-ELLKTMVEKGFKGRKTGKGCYVYQ 641

HSP 2 Score: 58.151 bits (139), Expect = 1.962e-8
Identity = 24/50 (48.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFASNTS+L I D+A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  464 EAVIPPHCIFASNTSALPIKDIAAASKRPEKVIGMHYFSPVDKMQLLEII 513
[Close]
BLAST of SMED30014769 vs. Ensembl Cavefish
Match: hadhab (trifunctional enzyme subunit alpha, mitochondrial-like [Source:NCBI gene;Acc:103035446])

HSP 1 Score: 60.077 bits (144), Expect = 4.591e-9
Identity = 41/111 (36.94%), Postives = 58/111 (52.25%), Query Frame = -2 
Query:  129 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKF-PNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYS 458
            GK  +   D PGF   R L P + EA+R+L+ G    + +D ++  G G+P+G   LAD VG+D    + +   + F P     NP  LL  MV  G  G+K+GKG Y Y 
Sbjct:  520 GKIIIVVGDGPGFYTTRCLAPMLAEAVRVLQEG-VDPKKLD-SLTTGFGFPVGAATLADEVGIDVAAHVAEDLGKAFGPRLGGGNP-ELLKTMVEKGFKGRKTGKGCYVYQ 627

HSP 2 Score: 58.151 bits (139), Expect = 1.914e-8
Identity = 24/50 (48.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  CIFASNTS+L I D+A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  450 EAVIPPHCIFASNTSALPIKDIAAASKRPEKVIGMHYFSPVDKMQLLEII 499
[Close]
BLAST of SMED30014769 vs. Ensembl Sea Lamprey
Match: hadh (hydroxyacyl-CoA dehydrogenase [Source:ZFIN;Acc:ZDB-GENE-040801-261])

HSP 1 Score: 167.933 bits (424), Expect = 9.124e-48
Identity = 75/122 (61.48%), Postives = 94/122 (77.05%), Query Frame = -2 
Query:  129 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYS 494
             ++AL  F  ++GK  V C+DTPGFIVNRLLVPY+ EA+R+ ERG  + EDID+AM+LGAGYPMGP  L DYVGLDT KFI+DGWH+  P NPLF P  +L+K+V  G  G+KSG+GFY Y+
Sbjct:  195 TFEALTDFTIALGKKPVSCKDTPGFIVNRLLVPYMMEAVRLHERGHGSKEDIDLAMKLGAGYPMGPFELLDYVGLDTGKFIIDGWHQLEPENPLFAPSKMLNKLVEEGKFGRKSGEGFYKYA 316

HSP 2 Score: 85.8853 bits (211), Expect = 1.201e-18
Identity = 43/61 (70.49%), Postives = 46/61 (75.41%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            D  AP   IFASNTSSL I D+A +T+RQDRFGGLHFFNPVPVM LVEVV T  TS  T E
Sbjct:  137 DKFAPEETIFASNTSSLPITDIANSTSRQDRFGGLHFFNPVPVMKLVEVVETPMTSKATFE 197
[Close]
BLAST of SMED30014769 vs. Ensembl Sea Lamprey
Match: hadhab (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha b [Source:ZFIN;Acc:ZDB-GENE-041111-204])

HSP 1 Score: 72.7886 bits (177), Expect = 9.249e-14
Identity = 42/115 (36.52%), Postives = 64/115 (55.65%), Query Frame = -2 
Query:  114 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 458
            GK  +  +D PGF   R L P + EA+R L+ G  + + +D ++  G G+P+G  +LAD VGLD    + +   + F +      +G+L  MV+ G LG+KSGKG Y Y    K+
Sbjct:  538 GKVIIIVKDGPGFYTTRCLAPMLAEAIRTLQEG-ISPKKLD-SLTTGFGFPVGAASLADEVGLDVAAHVAEDLEKAFGSRFGGGDVGVLKTMVSKGFLGRKSGKGCYVYQPGKKE 650

HSP 2 Score: 56.225 bits (134), Expect = 1.333e-8
Identity = 23/50 (46.00%), Postives = 36/50 (72.00%), Query Frame = -1 
Query:  793 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVV 942
            +A  P  C+FASNTS+L IA +A A+ R ++  G+H+F+PV  M L+E++
Sbjct:  468 EAVIPPHCVFASNTSALPIAKIAAASKRPEKVIGMHYFSPVDKMQLLEII 517
[Close]
BLAST of SMED30014769 vs. Ensembl Nematostella
Match: EDO46266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQC1])

HSP 1 Score: 168.703 bits (426), Expect = 1.310e-47
Identity = 77/123 (62.60%), Postives = 94/123 (76.42%), Query Frame = -2 
Query:  126 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSS 494
             +  LL F K +GK  V C+DTPGFIVNRLLVPY+ EA+RMLERG+A+ +DID AM+LGAGYPMGP  LADYVGLDTT+FIL GW EK+P+  LF     +  +VA   LG+KSG+GFY+Y S
Sbjct:  209 TFNTLLTFGKKLGKVPVSCKDTPGFIVNRLLVPYLAEAVRMLERGDASPKDIDTAMKLGAGYPMGPFELADYVGLDTTQFILKGWEEKYPDVELFKCPEFIKDLVARNKLGRKSGEGFYNYKS 331

HSP 2 Score: 92.0485 bits (227), Expect = 2.143e-20
Identity = 42/59 (71.19%), Postives = 47/59 (79.66%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D AAP  CIFA+NTSS+ +AD++  TNR DRFGGLHFFNPVPVM LVEVV T  TS DT
Sbjct:  151 DEAAPPQCIFATNTSSIPVADISSVTNRHDRFGGLHFFNPVPVMKLVEVVRTQQTSDDT 209
[Close]
BLAST of SMED30014769 vs. Ensembl Nematostella
Match: EDO28860 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5A3])

HSP 1 Score: 74.3294 bits (181), Expect = 2.874e-14
Identity = 40/109 (36.70%), Postives = 61/109 (55.96%), Query Frame = -2 
Query:  132 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 458
            GKT +  +D PGF   R+L P + EA+ +L+ G    +   ++ + G  +P+G + LAD VGLD    + +   +          I +L  +VAGGNLG+KSGKGF+ Y
Sbjct:  177 GKTVITVKDGPGFYTTRILAPALAEAVALLQEGVGPKQLDSLSKQFG--FPVGSVTLADEVGLDVACHVAEDLEKALGARLGGADINVLKSLVAGGNLGRKSGKGFFVY 283

HSP 2 Score: 62.7734 bits (151), Expect = 1.275e-10
Identity = 27/55 (49.09%), Postives = 39/55 (70.91%), Query Frame = -1 
Query:  766 PSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 930
            P  C+FASNTSSL I  +A A+ R ++  G+H+F+PV  M L+E+++T  TS DT
Sbjct:  111 PEHCVFASNTSSLPITQIAEASKRPEKVIGMHYFSPVDKMPLLEIITTEKTSKDT 165
[Close]
BLAST of SMED30014769 vs. Ensembl Nematostella
Match: EDO29655 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T309])

HSP 1 Score: 60.4622 bits (145), Expect = 4.132e-10
Identity = 29/59 (49.15%), Postives = 39/59 (66.10%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            + +A S  + ASNTS+L I  +A A  RQD+F GLHFF+PV  M LVE++    TS +T
Sbjct:  102 ERSALSDAVIASNTSTLPITGLATAVQRQDKFIGLHFFSPVDKMPLVEIIKGEQTSDET 160

HSP 2 Score: 48.521 bits (114), Expect = 3.086e-6
Identity = 36/127 (28.35%), Postives = 59/127 (46.46%), Query Frame = -2 
Query:  114 FVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFI-------LDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 473
            +V  + KT +   D+ GF  +R+   +  E L ML  G +     + A +  AG P+GPLA++D V +     I       L    ++ P +  F  I ++  +      G+ +G GFYDY +  KK
Sbjct:  167 YVMQIKKTPIVVNDSRGFFTSRVFGTFTNEGLAMLGEGVSAALIENEARK--AGMPVGPLAISDEVSMSLMNHIRQQTIKDLAAEGKQIPAHSAFAVIDMM--LNEYKRPGKAAGAGFYDYPAGGKK 289
[Close]
BLAST of SMED30014769 vs. Ensembl Nematostella
Match: EDO29372 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3U0])

HSP 1 Score: 54.6842 bits (130), Expect = 4.654e-8
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = -1 
Query:  775 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTS 942
            DA    G I ASNTS+L I  +A  T R ++  GLHFF+P  +M L+E+V  G T+
Sbjct:  109 DATLKPGAILASNTSALDIDAIAAVTKRPEQVLGLHFFSPAHIMKLLEIVRGGQTA 164

HSP 2 Score: 51.9878 bits (123), Expect = 3.231e-7
Identity = 38/119 (31.93%), Postives = 60/119 (50.42%), Query Frame = -2 
Query:  114 KSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAG-GNLGQKSGKGFYDYSSKAKK 467
            + +GK +V   +  GFI NR+L  Y+ EA RML    A    +D A++ G G+ MGP  + D VG+D     L+    +       +P   +D  + G G  GQK   G+Y Y+  +++
Sbjct:  176 QRIGKVSVVAGNCDGFIGNRMLHTYVREA-RMLLLEGAYPHQVDAALQ-GFGFAMGPFRMYDVVGID-----LEWRARELAGKGQDDPTVQVDNRLCGLGRFGQKVRMGYYLYAEGSRQ 287
[Close]
BLAST of SMED30014769 vs. Ensembl Medaka
Match: hadh (hydroxyacyl-CoA dehydrogenase [Source:NCBI gene;Acc:101166688])

HSP 1 Score: 170.629 bits (431), Expect = 2.796e-48
Identity = 78/121 (64.46%), Postives = 95/121 (78.51%), Query Frame = -2 
Query:  132 NWQALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 494
             + +LL F K++GKT V C+DTPGFIVNRLLVPY+ EALR+ ERG  + EDID+AM+LGAGYPMGP  L DYVGLDT KFI+DGW  K P+NPLF    LL+K+VA G  G+K+G+GFY Y
Sbjct:  188 TFDSLLNFSKALGKTPVSCKDTPGFIVNRLLVPYMMEALRLHERGHGSKEDIDIAMKLGAGYPMGPFELCDYVGLDTVKFIIDGWSAKDPHNPLFASSELLNKLVAEGKYGKKTGEGFYKY 308

HSP 2 Score: 83.9593 bits (206), Expect = 2.147e-17
Identity = 40/59 (67.80%), Postives = 46/59 (77.97%), Query Frame = -1 
Query:  766 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDT 942
            D  AP+  IFASNTSSL I D+A +T+R DRFGGLHFFNPVP+M LVEVV T  TS +T
Sbjct:  130 DKLAPANTIFASNTSSLPITDIASSTSRLDRFGGLHFFNPVPMMKLVEVVGTSTTSQET 188
[Close]
BLAST of SMED30014769 vs. Ensembl Medaka
Match: hadhab (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:101155646])

HSP 1 Score: 69.707 bits (169), Expect = 5.055e-12
Identity = 30/60 (50.00%), Postives = 43/60 (71.67%), Query Frame = -1 
Query:  763 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTT 942
            +A  P  CIFASNTS+L I ++A A+ R D+  G+H+F+PV  M L+E+++T  TS DTT
Sbjct:  463 EAVVPPHCIFASNTSALPIKEIAAASKRPDKVVGMHYFSPVDKMQLLEIITTEKTSKDTT 522

HSP 2 Score: 59.6918 bits (143), Expect = 5.656e-9
Identity = 39/114 (34.21%), Postives = 57/114 (50.00%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +   D PGF   R L P + EA+R+L+ G    + +D A+    G+P+G   LAD VG+D    + +   + F +      + +L  MV  G  G+KSGKG Y Y    K
Sbjct:  533 GKVIIVVGDGPGFYTTRCLAPMLAEAVRVLQEG-VDPKKLD-ALTTSFGFPVGAATLADEVGIDVAAHVAEDLGKAFGSRFGGGNVEVLKTMVDMGFKGRKSGKGCYIYQPGTK 644
[Close]
BLAST of SMED30014769 vs. Ensembl Medaka
Match: hadhab (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:101155646])

HSP 1 Score: 69.3218 bits (168), Expect = 5.264e-12
Identity = 30/60 (50.00%), Postives = 43/60 (71.67%), Query Frame = -1 
Query:  763 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTT 942
            +A  P  CIFASNTS+L I ++A A+ R D+  G+H+F+PV  M L+E+++T  TS DTT
Sbjct:  463 EAVVPPHCIFASNTSALPIKEIAAASKRPDKVVGMHYFSPVDKMQLLEIITTEKTSKDTT 522

HSP 2 Score: 59.6918 bits (143), Expect = 5.425e-9
Identity = 39/114 (34.21%), Postives = 57/114 (50.00%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +   D PGF   R L P + EA+R+L+ G    + +D A+    G+P+G   LAD VG+D    + +   + F +      + +L  MV  G  G+KSGKG Y Y    K
Sbjct:  533 GKVIIVVGDGPGFYTTRCLAPMLAEAVRVLQEG-VDPKKLD-ALTTSFGFPVGAATLADEVGIDVAAHVAEDLGKAFGSRFGGGNVEVLKTMVDMGFKGRKSGKGCYIYQPGTK 644
[Close]
BLAST of SMED30014769 vs. Ensembl Medaka
Match: hadhab (hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha [Source:NCBI gene;Acc:101155646])

HSP 1 Score: 69.3218 bits (168), Expect = 6.120e-12
Identity = 30/60 (50.00%), Postives = 43/60 (71.67%), Query Frame = -1 
Query:  763 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTT 942
            +A  P  CIFASNTS+L I ++A A+ R D+  G+H+F+PV  M L+E+++T  TS DTT
Sbjct:  517 EAVVPPHCIFASNTSALPIKEIAAASKRPDKVVGMHYFSPVDKMQLLEIITTEKTSKDTT 576

HSP 2 Score: 59.6918 bits (143), Expect = 6.850e-9
Identity = 39/114 (34.21%), Postives = 57/114 (50.00%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GK  +   D PGF   R L P + EA+R+L+ G    + +D A+    G+P+G   LAD VG+D    + +   + F +      + +L  MV  G  G+KSGKG Y Y    K
Sbjct:  587 GKVIIVVGDGPGFYTTRCLAPMLAEAVRVLQEG-VDPKKLD-ALTTSFGFPVGAATLADEVGIDVAAHVAEDLGKAFGSRFGGGNVEVLKTMVDMGFKGRKSGKGCYIYQPGTK 698
[Close]
BLAST of SMED30014769 vs. Ensembl Medaka
Match: ehhadh (enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase [Source:NCBI gene;Acc:101170799])

HSP 1 Score: 55.0694 bits (131), Expect = 1.665e-7
Identity = 37/121 (30.58%), Postives = 60/121 (49.59%), Query Frame = -2 
Query:  132 KSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFIL---------DGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDY 467
            K +GK +V   +  GF+ NR+L PY+ +A  +LE G  T E +D  +    G+ MG   ++D  GLD    +          +G   +      ++P+G  D +   G  GQK+G+G+Y Y
Sbjct:  460 KQMGKVSVAVGNCRGFVGNRMLKPYLEQAFFLLEEG-TTPEFVDQVLE-EFGFAMGVFRMSDLSGLDVGWRVRKADGLLNASEGHSARIRQGCRYSPLG--DLLCEQGRFGQKTGRGWYQY 576
[Close]
BLAST of SMED30014769 vs. Planmine SMEST
Match: SMESG000032777.1 (SMESG000032777.1)

HSP 1 Score: 263.848 bits (673), Expect = 4.519e-84
Identity = 123/125 (98.40%), Postives = 125/125 (100.00%), Query Frame = -2 
Query:  114 QALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 488
            +ALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYP+GPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK
Sbjct:  169 EALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPIGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 293

HSP 2 Score: 128.257 bits (321), Expect = 5.445e-33
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = -1 
Query:  760 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 942
            DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE
Sbjct:  109 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE 169
[Close]
BLAST of SMED30014769 vs. Planmine SMEST
Match: SMESG000032777.1 (SMESG000032777.1)

HSP 1 Score: 263.848 bits (673), Expect = 8.554e-84
Identity = 123/125 (98.40%), Postives = 125/125 (100.00%), Query Frame = -2 
Query:  114 QALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 488
            +ALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYP+GPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK
Sbjct:  189 EALLAFVKSVGKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPIGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAKK 313

HSP 2 Score: 127.872 bits (320), Expect = 9.798e-33
Identity = 67/80 (83.75%), Postives = 70/80 (87.50%), Query Frame = -1 
Query:  706 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTEVSGRGHVAIV-AIGGHAVR 942
            DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE      +A V ++G  AVR
Sbjct:  129 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTE----ALLAFVKSVGKTAVR 204
[Close]
BLAST of SMED30014769 vs. Planmine SMEST
Match: SMESG000061749.1 (SMESG000061749.1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.169e-11
Identity = 42/114 (36.84%), Postives = 57/114 (50.00%), Query Frame = -2 
Query:  117 GKTAVRCRDTPGFIVNRLLVPYIGEALRMLERGEATCEDIDVAMRLGAGYPMGPLALADYVGLDTTKFILDGWHEKFPNNPLFNPIGLLDKMVAGGNLGQKSGKGFYDYSSKAK 458
            GKT +   D PGF   R+L P + EA+R+L+ G +  E   +      G+P+G + LAD VGLD    I       F        + +L  MVA G LG+K+ KG Y Y  K K
Sbjct:  527 GKTVIVVGDGPGFYTTRILAPMMSEAVRVLQEGVSPAELDKITKDF--GWPVGTVTLADEVGLDVASHISIDLGTAFGKRMDGGDVNILKDMVAAGFLGKKNKKGCYVYDGKLK 638

HSP 2 Score: 54.299 bits (129), Expect = 1.955e-7
Identity = 22/63 (34.92%), Postives = 41/63 (65.08%), Query Frame = -1 
Query:  754 DAAAPSGCIFASNTSSLSIADMAVATNRQDRFGGLHFFNPVPVMGLVEVVSTGNTSTDTTEVS 942
            ++  P   +FA+NTS+L I ++A  + R ++  G+H+F+PV  M L+E+++T  T  +T  V+
Sbjct:  457 ESCLPDNVVFATNTSALPIHEIASVSKRPNKIIGMHYFSPVEKMPLLEIITTDKTDEETKAVA 519
The following BLAST results are available for this feature:
BLAST of SMED30014769 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HADH1.750e-4756.93hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;... [more]
HADH2.859e-4761.98hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;... [more]
HADH5.300e-4761.98hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;... [more]
HADH2.129e-4661.98hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;... [more]
HADH5.388e-4560.48hydroxyacyl-CoA dehydrogenase [Source:HGNC Symbol;... [more]
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BLAST of SMED30014769 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
B0272.32.447e-4859.70Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 ... [more]
F54C8.17.598e-4559.50Probable 3-hydroxyacyl-CoA dehydrogenase F54C8.1 ... [more]
hacd-19.500e-3855.56Hydroxy-Acyl-CoA Dehydrogenase [Source:UniProtKB/... [more]
ech-1.25.892e-934.21Enoyl-CoA Hydratase [Source:UniProtKB/TrEMBL;Acc:... [more]
ech-1.26.695e-934.21Enoyl-CoA Hydratase [Source:UniProtKB/TrEMBL;Acc:... [more]
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BLAST of SMED30014769 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mtpalpha1.923e-831.58gene:FBgn0028479 transcript:FBtr0079857[more]
Mtpalpha2.122e-831.58gene:FBgn0028479 transcript:FBtr0079858[more]
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BLAST of SMED30014769 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
hadh1.277e-4965.29hydroxyacyl-CoA dehydrogenase [Source:ZFIN;Acc:ZDB... [more]
hadhaa2.116e-1035.65hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadhab1.225e-933.91hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
ehhadh1.228e-832.06enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogena... [more]
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BLAST of SMED30014769 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
vps416.617e-5165.29VPS41, HOPS complex subunit [Source:Xenbase;Acc:XB... [more]
vps416.982e-5165.29VPS41, HOPS complex subunit [Source:Xenbase;Acc:XB... [more]
hadha2.166e-1238.60hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadha2.290e-1238.60hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadha2.315e-1238.60hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
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BLAST of SMED30014769 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Hadh6.169e-4963.64hydroxyacyl-Coenzyme A dehydrogenase [Source:MGI S... [more]
Hadha2.004e-1336.84hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Co... [more]
Ehhadh1.325e-830.47enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme... [more]
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BLAST of SMED30014769 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9WVK7|HCDH_RAT2.766e-4863.64Hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
sp|Q61425|HCDH_MOUSE4.315e-4863.64Hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
sp|P41938|HCDH2_CAEEL3.113e-4759.70Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 O... [more]
sp|P00348|HCDH_PIG6.328e-4761.98Hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
sp|Q16836|HCDH_HUMAN2.545e-4661.98Hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
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BLAST of SMED30014769 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267G4S86.678e-5368.25Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1S3H0U71.506e-5268.55hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
A0A068FKF71.576e-5267.74Hydroxyacyl-CoA dehydrogenase OS=Agrotis segetum O... [more]
A0A4C1TVX21.990e-5266.67Hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
A0A1S3HUR32.574e-5268.29hydroxyacyl-coenzyme A dehydrogenase, mitochondria... [more]
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BLAST of SMED30014769 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hadh1.571e-4964.46hydroxyacyl-CoA dehydrogenase [Source:NCBI gene;Ac... [more]
hadhaa2.116e-1038.14hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadhaa2.145e-1038.14hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadhab4.357e-936.94trifunctional enzyme subunit alpha, mitochondrial-... [more]
hadhab4.591e-936.94trifunctional enzyme subunit alpha, mitochondrial-... [more]
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BLAST of SMED30014769 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hadh9.124e-4861.48hydroxyacyl-CoA dehydrogenase [Source:ZFIN;Acc:ZDB... [more]
hadhab9.249e-1436.52hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
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BLAST of SMED30014769 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014769 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO462661.310e-4762.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO288602.874e-1436.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO296554.132e-1049.15Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO293724.654e-846.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30014769 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hadh2.796e-4864.46hydroxyacyl-CoA dehydrogenase [Source:NCBI gene;Ac... [more]
hadhab5.055e-1250.00hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadhab5.264e-1250.00hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
hadhab6.120e-1250.00hydroxyacyl-CoA dehydrogenase trifunctional multie... [more]
ehhadh1.665e-730.58enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrog... [more]
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BLAST of SMED30014769 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000032777.14.519e-8498.40SMESG000032777.1[more]
SMESG000032777.18.554e-8498.40SMESG000032777.1[more]
SMESG000061749.12.169e-1136.84SMESG000061749.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014769 ID=SMED30014769|Name=SMED30014769|organism=Schmidtea mediterranea sexual|type=transcript|length=1668bp
AGAAACCGATAGGAGCATCCTGAAACGATTTCCATGGCAATAAAAACAAA
CAATTGAAAAAAAGTCGAATTTATAAAATAAAAATGAATTCCAAATCATA
AAAATCGGATTTATTTCTTAGCTTTGCTGGAGTAATCATAGAATCCCTTT
CCGGATTTCTGTCCGAGGTTCCCGCCTGCCACCATTTTGTCCAGCAGCCC
AATCGGGTTGAATAGCGGGTTGTTGGGGAACTTTTCATGCCAGCCATCCA
GGATGAACTTGGTGGTGTCGAGGCCCACATAATCCGCCAGTGCCAGCGGC
CCCATCGGGTACCCGGCACCCAGTCGCATGGCAACATCGATATCCTCACA
CGTGGCCTCCCCGCGCTCCAGCATGCGCAGCGCCTCACCGATATACGGCA
CCAATAACCGATTGACAATAAATCCCGGCGTGTCGCGGCACCTGACCGCA
GTCTTCCCCACCGATTTCACAAACGCCAGCAGAGCCTGCCAATTATATAT
TATATATATTTGTGTGTGTGTGTGAGAGGCAACTGGGGCACTCGCATCTG
ATTGGCCGCCACAAATGGGAGGGTTACAGAGGCCCCTTGTGCAATATTGG
CACGCATTTCAGGGTTAGGTAGGAGTTTGGTGGGCCAGTGGTGCCCCTTG
GTTATTGGCGCAGTTATTGCCATGGCAACTGTCGGTGCCTCATTATGCCT
TCATCCCGCACCGCATGGCCACCAATTGCCACAATCGCAACATGCCCCCT
CCCACTCACCTCAGTCGTGTCTGTGCTGGTGTTGCCAGTGGAAACCACCT
CAACCAGCCCCATCACCGGCACCGGATTGAAGAAATGCAGCCCACCGAAT
CTGTCCTGCCGGTTGGTTGCCACAGCCATATCCGCAATCGATAGCGAGGA
AGTGTTGCTGGCAAATATGCAGCCCGATGGCGCCGCTGCATCGAGACTCC
TGGAACAAAGCAATGATAACATTAATAGTACAACGCTGGAAATTAATGGA
TTATCGGTTGATTACGTTTCCCAAGGACGTGCGGAGGACACAGGAGCTGC
TGATGTCCATCTGCATGCATTGCACAGCCCTTTACGTCCCCCTGATGACC
GCAACTGGGACACAGGGGCAGATGCAGCCCTCTCGCCCCCCCCCACCTAC
AGCAACCGATAAGCTATCAGCAGGTGTATCGACATCCCTAGCAACAACGG
GTCGGACAATGAAGGAAACGCGACTCAATTCCACTCACAAATCCCCACAA
TCGTGTATGACAGCCAATCATTGGCTAGCACAAATCAACTCAATAATTAT
CCGCAACAACGCTCTCGACTTTGACCGCAATATCACGAATCGGTTACATG
AGAGTTCACAGGTCAATCAATGATCATAGCAAGCGCCAGGCAGACAATGT
GACACGTGTAAATGCAATTACATCGTCTCACTGGGGCCGGAATATTGCTC
ATTTCTCCACGATTCCTTCTGCAGCTACTGAAAATTTGCTGCTTCCATAA
GCAAATAAATAATTTATTTATGCTTTTACAGCACAGCCAAACTGTGCAAT
GCAAATTAATGTTAACAGTTCTAAGATAATATAATTTCACTTACGTAACA
TTCATGACATCATAATAATTATATATAAATATGAAAATTAAAATATAAAA
TTTAATCACAAAATCTAA
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protein sequence of SMED30014769-orf-1

>SMED30014769-orf-1 ID=SMED30014769-orf-1|Name=SMED30014769-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
MPSSRTAWPPIATIATCPLPLTSVVSVLVLPVETTSTSPITGTGLKKCSP
PNLSCRLVATAISAIDSEEVLLANMQPDGAAASRLLEQSNDNINSTTLEI
NGLSVDYVSQGRAEDTGAADVHLHALHSPLRPPDDRNWDTGADAALSPPP
TYSNR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000070intestinal phagocyte
PLANA:0000240body wall musculature
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR0133286PGD_dom2
IPR0061083HC_DH_C
IPR0061803-OHacyl-CoA_DH_CS
IPR0089276-PGluconate_DH-like_C_sf
IPR016040NAD(P)-bd_dom
IPR0061763-OHacyl-CoA_DH_NAD-bd
Vocabulary: molecular function
TermDefinition
GO:0016491oxidoreductase activity
GO:00038573-hydroxyacyl-CoA dehydrogenase activity
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006631fatty acid metabolic process