SMED30014756

Overview
NameSMED30014756
Smed IDSMED30014756
Length (bp)1394
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014756

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 18

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30014756h1SMcG0019979 SmedASXL_009679SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30014756h1SMcG0019979 SmedASXL_007896SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30014756h1SMcG0019979 SmedASXL_009679SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30014756h1SMcG0019979 dd_131dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
gutSMED30014756h1SMcG0019980 dd_131dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Smed sexual biotypeSMED30014756h1SMcG0019979 Contig7310newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014756h1SMcG0019979 Contig7310uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014756h1SMcG0019979 Contig19969uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014756h1SMcG0019979 Contig19969newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30014756h1SMcG0019979 dd_Smed_v4_2506_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014756h1SMcG0019979 dd_Smed_v4_1491_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30014756h1SMcG0019979 dd_Smed_v6_2506_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30014756h1SMcG0019979 dd_Smed_v6_1491_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
goblet cellSMED30014756h1SMcG0019980 dd_Smed_v6_131_0dd_Smed_v6PMID:29674432
Plass et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
goblet cellSMED30014756h1SMcG0019980 dd_Smed_v6_131_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30014756h1SMcG0019979 dd_Smed_v6_1491_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30014756h1SMcG0019979 dd_Smed_v6_131_0dd_Smed_v6PMID:29674432
Plass et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
goblet cellSMED30014756h1SMcG0019979 dd_Smed_v6_131_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014756 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.006101:3366..7772 +6103
v31.006101:12236..15535 -4334
Homology
[Close]
BLAST of SMED30014756 vs. Planmine SMEST
Match: SMESG000035313.1 (SMESG000035313.1)

HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0
Identity = 408/421 (96.91%), Postives = 412/421 (97.86%), Query Frame = 3 
Query:   63 MIAYFLLFLTISLVSSTPLEQNELKLQERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKESTF 1325
            MI+YF+L LTISLVS    E   L+ +ERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKESTF
Sbjct:    1 MISYFILALTISLVSCDTFEARSLEFEERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKESTF 421
[Close]
BLAST of SMED30014756 vs. Planmine SMEST
Match: SMESG000035312.1 (SMESG000035312.1)

HSP 1 Score: 704.516 bits (1817), Expect = 0.000e+0
Identity = 347/418 (83.01%), Postives = 391/418 (93.54%), Query Frame = 3 
Query:   63 MIAYFLLFLTISLVSSTPLEQNELKLQERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKE 1316
            MIAYFLLFLTISLVSSTPLEQNE KLQERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGIL+HLGPLSRKIADGIINQFREL++K+DEATKEKTE++KRDFKESS+ LLKKL +IL +AF+KGS AF +A+ DPDVKEILNSL  +SRRTANF+IEKFKELVIK+DE T EK K+ERGLI+++  N K LIQILI AS++G+EYF  I++DPD +AI+E+LN +EQ+II+SII+KF++IFDKID+ATKP SD P+AVSDW+EDA  QLK+LTQILLKAYR+GGEYF+KV+ND DVQNILQHLGPLTR++A S+I+NFRELIEKIDNATKE
Sbjct:    1 MIAYFLLFLTISLVSSTPLEQNEFKLQERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILDHLGPLSRKIADGIINQFRELLEKLDEATKEKTEIQKRDFKESSISLLKKLTHILTQAFQKGSAAFNEALEDPDVKEILNSLSGISRRTANFMIEKFKELVIKIDEATNEKQKTERGLIDNTTKNVKNLIQILIKASKQGSEYFRTILNDPDVIAIREHLNILEQRIIDSIIKKFIEIFDKIDQATKPESDYPYAVSDWKEDAKDQLKRLTQILLKAYRKGGEYFDKVVNDPDVQNILQHLGPLTRKVANSIIENFRELIEKIDNATKE 418

HSP 2 Score: 139.043 bits (349), Expect = 2.453e-36
Identity = 75/157 (47.77%), Postives = 109/157 (69.43%), Query Frame = 3 
Query:  852 ERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKEST 1322
            ER   E S +   +LI+ L  A  KGAE F  +I+D D   I ++++++ +++  SII KF ++  KID+ATKP S+   +  + +E +T  +K+L QILL AY+QG EYF++V+NDSDVQ IL HLGPL+R+IA  +I+ FREL+EK+D ATKE T
Sbjct:   28 ERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEP--SKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILDHLGPLSRKIADGIINQFRELLEKLDEATKEKT 182
[Close]
BLAST of SMED30014756 vs. Planmine SMEST
Match: SMESG000035313.1 (SMESG000035313.1)

HSP 1 Score: 649.432 bits (1674), Expect = 0.000e+0
Identity = 328/393 (83.46%), Postives = 360/393 (91.60%), Query Frame = 3 
Query:  147 RYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKESTF 1325
            R  KE S+ L+  L++ L  AY++G E FDRV+NDSD++GIL+H+  L+R++A+ IIN+F+ELI+KIDEATK      KR+  ESS +L+K+L +ILL AY +G EY  +V+ND +V+ IL+HLGPLSR++A+ IIN FRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKESTF
Sbjct:  108 RNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIEKIDEATKETQTNEKRS--ESSIELLKKLNEILLKAYNKGGEYVAQVINDPNVKAILDHLGPLSRRVAESIINNFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKESTF 498

HSP 2 Score: 484.952 bits (1247), Expect = 6.879e-169
Identity = 271/423 (64.07%), Postives = 317/423 (74.94%), Query Frame = 3 
Query:   63 MIAYFLLFLTISLVSSTPLEQNELKLQERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSE-RGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKAT--KPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKEST 1322
            MI+YF+L LTISLVS    E   L+ +ERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELI+KIDEATKE    EKR   ESS+ LLKKL  IL+KA+ KG E   + INDP+VK IL+ L  LSRR A  II  F+EL+ K+DE T EK + E R   E S    KKL+ IL+ A  KG+E F   I+DPD   I  +L+S+ ++  N IIEKF ++  K+D+ T  KP S+         ED+    KKL QIL+ A R+G EYF  +I+D D   I ++L  + ++I  S+I+ F ++ +KID ATK ++
Sbjct:    1 MISYFILALTISLVSCDTFEARSLEFEERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIEKIDEATKETQTNEKRS--ESSIELLKKLNEILLKAYNKGGEYVAQVINDPNVKAILDHLGPLSRRVAESIINNFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLI-----EDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNS 416

HSP 3 Score: 140.198 bits (352), Expect = 3.188e-36
Identity = 77/157 (49.04%), Postives = 111/157 (70.70%), Query Frame = 3 
Query:  144 ERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISE-P-SKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKT 608
            ER   E S +   +LI+ L  A  KGAE F  +I+D D   I ++++++ +++  SII KF ++  KID+ATKP S+ P +  + +E +T  +K+L QILL AY+QG EYF++V+NDSDVQ IL+HLGPL+R+IA  +I+ FRELI+KID ATKE T
Sbjct:  341 ERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKEST 497
[Close]
BLAST of SMED30014756 vs. Planmine SMEST
Match: SMESG000035252.1 (SMESG000035252.1)

HSP 1 Score: 84.7297 bits (208), Expect = 8.221e-20
Identity = 39/71 (54.93%), Postives = 55/71 (77.46%), Query Frame = 3 
Query:  384 RNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEAT 596
            +  KESS  L+K+L +IL +AY +G E F +V+ND DV+ ILE LGPLS+K+A+ II  F+EL++KID+AT
Sbjct:   27 KEFKESSVNLIKKLTKILKDAYSKGSESFHKVLNDPDVKAILERLGPLSKKVANSIIKSFKELVQKIDKAT 97

HSP 2 Score: 82.8037 bits (203), Expect = 4.095e-19
Identity = 44/103 (42.72%), Postives = 69/103 (66.99%), Query Frame = 3 
Query:   63 MIAYFLLFLTISLVSSTPLEQNELKLQERYFKEKSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPIS 371
            MI YF+    +SLV S   + ++  ++E  FKE S +L+ +L + L+ AY KG+E F +V+ND D+K IL+ +  L++++A SII  FKEL++KID+AT   S
Sbjct:    1 MIKYFIFLALLSLVLSDDKKTDDSTVKE--FKESSVNLIKKLTKILKDAYSKGSESFHKVLNDPDVKAILERLGPLSKKVANSIIKSFKELVQKIDKATGKTS 101

HSP 3 Score: 82.0333 bits (201), Expect = 6.502e-19
Identity = 42/71 (59.15%), Postives = 54/71 (76.06%), Query Frame = 3 
Query:  621 RDFKESSVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELT 833
            ++FKESSV L+KKL  IL  A+ KGSE+F+K +NDPDVK IL  L  LS++ AN II+ FKELV K+D+ T
Sbjct:   27 KEFKESSVNLIKKLTKILKDAYSKGSESFHKVLNDPDVKAILERLGPLSKKVANSIIKSFKELVQKIDKAT 97

HSP 4 Score: 80.4925 bits (197), Expect = 2.757e-18
Identity = 36/77 (46.75%), Postives = 58/77 (75.32%), Query Frame = 3 
Query: 1092 AVSDWQEDATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATSLIDNFRELIEKIDNATKEST 1322
             V +++E +   +KKLT+IL  AY +G E F KV+ND DV+ IL+ LGPL++++A S+I +F+EL++KID AT +++
Sbjct:   25 TVKEFKESSVNLIKKLTKILKDAYSKGSESFHKVLNDPDVKAILERLGPLSKKVANSIIKSFKELVQKIDKATGKTS 101

HSP 5 Score: 59.3066 bits (142), Expect = 7.587e-11
Identity = 33/71 (46.48%), Postives = 45/71 (63.38%), Query Frame = 3 
Query:  867 EDSKNNFKKLIQILINASRKGAEYFINIISDPDFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNS 1079
            E S N  KKL +IL +A  KG+E F  +++DPD  AI E L  + +K+ NSII+ F ++  KIDKAT   S
Sbjct:   31 ESSVNLIKKLTKILKDAYSKGSESFHKVLNDPDVKAILERLGPLSKKVANSIIKSFKELVQKIDKATGKTS 101
[Close]
BLAST of SMED30014756 vs. Planmine SMEST
Match: SMESG000035316.1 (SMESG000035316.1)

HSP 1 Score: 72.0182 bits (175), Expect = 4.809e-14
Identity = 64/229 (27.95%), Postives = 104/229 (45.41%), Query Frame = 3 
Query:   63 MIAYFLLFLTISLVSSTPLEQNELKLQERYFKE----KSSSLLVELIRTLRTAYEKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATKPISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKK------------LVNILVKAFEKGSE 701
            M  Y    L+ SL    P +  +   Q    K     KSS LL + I  L   YEKG  E++ +  D  I+ +++ MD  T+ +AES++ +F++ I   D       +  KR   ++S  L      I  N  + G             + I  +   LSR+  + ++ + RE++ +IDEATK K   EKR+ K   V + K+            L++IL +A ++GSE
Sbjct:    1 MTFYLTFVLSFSLAFCNPYKGGQFSNQPETIKRQEIGKSSQLLSKFIVELAKLYEKGIYEYNEIFQDPKIRKLIEKMDEFTKLVAESLVIQFEDEIISRDSPKSEFDDFEKRYTYKTSKSLSLIQRSIKGNGIRSG------------EKEITNNFEQLSRRRTEFLVRKLREIVDQIDEATKLKAANEKRNIKLYKVNVYKRNSSPDQKGYIQDLIDILSEASKRGSE 217

HSP 2 Score: 62.003 bits (149), Expect = 1.193e-10
Identity = 53/196 (27.04%), Postives = 100/196 (51.02%), Query Frame = 3 
Query:  402 STKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILEHLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKR-DFKES-SVRLLKKLVNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELVIKLDELTTEKPKSERGLIE-------------DSKNNFKKLIQILINASRKGAEYFI 944
            S++L+ + +  L   Y++G+  ++ +  D  ++ ++E +   ++ +A+ ++ QF + I   D    E  + EKR  +K S S+ L+++ +        KG+      I   + KEI N+ + LSRR   F++ K +E+V ++DE T  K  +E+  I+             D K   + LI IL  AS++G+EY I
Sbjct:   39 SSQLLSKFIVELAKLYEKGIYEYNEIFQDPKIRKLIEKMDEFTKLVAESLVIQFEDEIISRDSPKSEFDDFEKRYTYKTSKSLSLIQRSI--------KGN-----GIRSGE-KEITNNFEQLSRRRTEFLVRKLREIVDQIDEATKLKAANEKRNIKLYKVNVYKRNSSPDQKGYIQDLIDILSEASKRGSEYLI 220
The following BLAST results are available for this feature:
BLAST of SMED30014756 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014756 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035313.10.000e+096.91SMESG000035313.1[more]
SMESG000035312.12.453e-3683.01SMESG000035312.1[more]
SMESG000035313.16.879e-16983.46SMESG000035313.1[more]
SMESG000035252.18.221e-2054.93SMESG000035252.1[more]
SMESG000035316.14.809e-1427.95SMESG000035316.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014756 ID=SMED30014756|Name=SMED30014756|organism=Schmidtea mediterranea sexual|type=transcript|length=1394bp
GCTTCGTAAACAGATAAATACTGTTTGTGTTTCAAAATATTACAAATTTA
CGATTAGGTAAAATGATTGCATACTTCCTTCTATTTTTGACAATTTCTTT
GGTATCCTCCACTCCTCTTGAGCAAAATGAATTGAAATTACAAGAAAGAT
ATTTTAAAGAGAAGTCATCAAGCTTATTGGTAGAACTGATCCGAACTCTC
CGAACTGCGTATGAGAAAGGTGCTGAAGAATTCGACAGAGTTATCAATGA
TTCTGATATCAAAGGAATTTTAGATCATATGGACGCACTGACCCGACGAC
TGGCGGAGTCTATCATCAATAAATTCAAAGAGCTGATCAAAAAAATCGAC
GAAGCAACAAAACCGATTTCCGAACCATCCAAGCGAAACCTAAAAGAAAG
CTCAACAAAGCTCATGAAGAGATTGGTCCAGATTTTATTGAATGCCTACA
AACAAGGATTGGAATATTTTGACAGAGTTGTCAATGATTCTGATGTTCAA
GGAATTCTCGAACATCTTGGACCTCTGTCAAGAAAGATCGCAGATGGAAT
TATCAACCAATTCAGAGAGTTGATTAAAAAAATCGACGAAGCTACGAAAG
AGAAAACTGAAGTTGAGAAACGAGATTTCAAGGAAAGTTCAGTCAGACTT
TTGAAAAAATTGGTAAATATTCTCGTAAAAGCATTTGAAAAAGGTTCCGA
AGCATTTTACAAGGCCATCAATGACCCGGACGTTAAAGAAATTCTTAATA
GCCTCGACAGCCTAAGTAGAAGAACAGCCAATTTTATTATCGAAAAATTT
AAAGAGTTGGTAATCAAGCTTGATGAGTTGACAACTGAAAAGCCGAAGTC
CGAAAGAGGATTAATTGAAGATTCGAAAAATAATTTCAAGAAATTGATTC
AAATTCTCATAAATGCTTCCAGGAAAGGAGCAGAATATTTCATTAATATC
ATCAGTGATCCAGATTTCGTAGCCATCCAAGAAAATCTAAATTCGATCGA
GCAGAAAATTATTAATTCAATTATTGAAAAGTTTTTACAAATTTTTGATA
AAATCGACAAAGCTACAAAACCGAATTCAGATGTCCCATTTGCTGTATCA
GATTGGCAAGAAGATGCAACAGTTCAACTCAAAAAATTGACACAAATTTT
ATTGAAGGCCTATAGACAAGGCGGAGAATATTTTGAAAAGGTCATCAACG
ACTCGGACGTACAAAATATCCTTCAACATCTTGGCCCATTGACGAGAAGA
ATTGCCACTTCCTTAATCGACAATTTTAGAGAGTTAATTGAAAAAATTGA
CAACGCAACCAAAGAGAGCACTTTTTAATCAAGCTTTAGTGGGTAATGAA
ACAAAATATGAAATCGACTTTGTATAAATACAAATTGCCTATAT
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protein sequence of SMED30014756-orf-1

>SMED30014756-orf-1 ID=SMED30014756-orf-1|Name=SMED30014756-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=422bp
MIAYFLLFLTISLVSSTPLEQNELKLQERYFKEKSSSLLVELIRTLRTAY
EKGAEEFDRVINDSDIKGILDHMDALTRRLAESIINKFKELIKKIDEATK
PISEPSKRNLKESSTKLMKRLVQILLNAYKQGLEYFDRVVNDSDVQGILE
HLGPLSRKIADGIINQFRELIKKIDEATKEKTEVEKRDFKESSVRLLKKL
VNILVKAFEKGSEAFYKAINDPDVKEILNSLDSLSRRTANFIIEKFKELV
IKLDELTTEKPKSERGLIEDSKNNFKKLIQILINASRKGAEYFINIISDP
DFVAIQENLNSIEQKIINSIIEKFLQIFDKIDKATKPNSDVPFAVSDWQE
DATVQLKKLTQILLKAYRQGGEYFEKVINDSDVQNILQHLGPLTRRIATS
LIDNFRELIEKIDNATKESTF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000067goblet cell
PLANA:0000419prepharyngeal region
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR016024ARM-type_fold
Vocabulary: molecular function
TermDefinition
GO:0005488binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.709