SMED30014746

Overview
NameSMED30014746
Smed IDSMED30014746
Length (bp)2244
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014746 (SMED30014746) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014746 (SMED30014746) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014746 (SMED30014746) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014746 (SMED30014746) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30014746

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30014746SMESG000040783.1 SmedASXL_014193SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30014746SMESG000040783.1 SmedASXL_014193SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30014746SMESG000040783.1 dd_Smed_v4_14673_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014746SMESG000061623.1 SMESG000053117.1 SMESG000042104.1 SMESG000040787.1 SMESG000040786.1 SMESG000040784.1 SMESG000040783.1 SMESG000022718.1 Contig36896newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014746SMESG000061623.1 SMESG000053117.1 SMESG000042104.1 SMESG000040787.1 SMESG000040786.1 SMESG000040784.1 SMESG000040783.1 SMESG000022718.1 Contig36896uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parapharyngeal regionSMED30014746SMESG000040783.1 dd_Smed_v6_14673_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30014746SMESG000040783.1 dd_Smed_v4_14673_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014746 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000538:95424..104093 -10987
Homology
[Close]
BLAST of SMED30014746 vs. Ensembl Human
Match: NDC80 (NDC80 kinetochore complex component [Source:HGNC Symbol;Acc:HGNC:16909])

HSP 1 Score: 51.2174 bits (121), Expect = 7.563e-6
Identity = 27/109 (24.77%), Postives = 48/109 (44.04%), Query Frame = 3 
Query:  102 DPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFL 428
            DPRPL D  F++   ++L +FL    Y H V        +  D   IF      +   + L D++   E+++ R F  + Y    SK+ + +     + P ++ A  +L
Sbjct:   82 DPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKF--EEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWL 188
[Close]
BLAST of SMED30014746 vs. TrEMBL
Match: A0A4V3SEE9 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006456 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.051e-8
Identity = 38/143 (26.57%), Postives = 67/143 (46.85%), Query Frame = 3 
Query:   33 KSLAPKKD-------PISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFV 440
            +SL P K        P     A      + D  P++   F+     +LI FLE+T+YP+ V + +    T  +V +IFEH V  +  +FRL       ED  ++   ++ Y +  S+ ++ +P    + P ++ A ++LR  V
Sbjct:   15 RSLPPNKQRQTTVSIPAPMMRASSAAPPRGDRGPVK--AFLNKAITELIGFLEATEYPNGVSTRLLQAPTSKEVFSIFEHIVRQIDPNFRLTGYSGKVEDTCIKILRTLNYPHALSRQYLATPGAAHAWPSILSALNWLRELV 155
[Close]
BLAST of SMED30014746 vs. TrEMBL
Match: A0A4Z2DHV1 (Kinetochore protein NDC80 isoform 1 OS=Schistosoma japonicum OX=6182 GN=EWB00_001067 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.544e-7
Identity = 44/147 (29.93%), Postives = 70/147 (47.62%), Query Frame = 3 
Query:   36 SLAPKKD------PISNTNAGRGNTDKSDPRPLRDPNFMKDVKQK---------LIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLR 431
            SL P+++      P+ N  A   +    + RPL      +DVK K         LI+FLE+TDY + +   I       ++ +IFEH V  +   FR++   + AE+  L     + Y+N  SK H+ +P    +   V+ AFD+LR
Sbjct:    6 SLQPERNSRMTGVPL-NIKARTSSIGSQEQRPL-----YRDVKSKANMTKMIGSLIQFLETTDYSNSLSPKILHSPNSKEIFSIFEHLVRQICPTFRIEHDGMKAEEVCLNTLKMLGYSNILSKHHLLAPGAPQAWNSVLAAFDWLR 146
[Close]
BLAST of SMED30014746 vs. TrEMBL
Match: A0A4Z2DH21 (Kinetochore protein NDC80 isoform 3 OS=Schistosoma japonicum OX=6182 GN=EWB00_001067 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.646e-7
Identity = 44/147 (29.93%), Postives = 70/147 (47.62%), Query Frame = 3 
Query:   36 SLAPKKD------PISNTNAGRGNTDKSDPRPLRDPNFMKDVKQK---------LIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLR 431
            SL P+++      P+ N  A   +    + RPL      +DVK K         LI+FLE+TDY + +   I       ++ +IFEH V  +   FR++   + AE+  L     + Y+N  SK H+ +P    +   V+ AFD+LR
Sbjct:    6 SLQPERNSRMTGVPL-NIKARTSSIGSQEQRPL-----YRDVKSKANMTKMIGSLIQFLETTDYSNSLSPKILHSPNSKEIFSIFEHLVRQICPTFRIEHDGMKAEEVCLNTLKMLGYSNILSKHHLLAPGAPQAWNSVLAAFDWLR 146
[Close]
BLAST of SMED30014746 vs. TrEMBL
Match: A0A183R3S5 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.022e-7
Identity = 32/93 (34.41%), Postives = 50/93 (53.76%), Query Frame = 3 
Query:  153 LIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLR 431
            LI+FLE+TDY + +   I       ++ +IFEH V  V   FR+++  I AE+  L +   + Y N  SK H+ +P    +   V+ AFD+ R
Sbjct:   54 LIQFLETTDYSNSLSPKILHSPNSKEIFSIFEHLVRQVEPSFRIENDGIKAEEVCLNYLKILGYPNILSKHHLLAPGAPQAWNSVLAAFDWFR 146
[Close]
BLAST of SMED30014746 vs. TrEMBL
Match: A0A5J4NKL5 (Kinetochore protein NDC80 OS=Paragonimus westermani OX=34504 GN=DEA37_0005186 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.441e-7
Identity = 32/101 (31.68%), Postives = 50/101 (49.50%), Query Frame = 3 
Query:  129 FMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLR 431
            FM      LI+FLE TDYP+ +   +    +  +V  +FEH V  +   FR+D   +  E+  L     + Y    SK ++ +P    + P V+GA D+LR
Sbjct:   47 FMNKALTNLIEFLEFTDYPNAISMRLLQSPSSKEVFCVFEHIVRQLSPCFRIDRPGVKVEEICLSTLRDLGYPFTLSKQYLATPGAPHAWPTVLGALDWLR 147
[Close]
BLAST of SMED30014746 vs. Ensembl Cavefish
Match: ndc80 (NDC80 kinetochore complex component [Source:NCBI gene;Acc:103037330])

HSP 1 Score: 54.299 bits (129), Expect = 4.022e-7
Identity = 36/123 (29.27%), Postives = 58/123 (47.15%), Query Frame = 3 
Query:   84 GNTDK-SDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAF 449
            G T+K  DPRPL D  F++   ++L +FL    +P  +        +  +   IFE   SL+   F+L  ++I  E++I R F  + Y    SK+ + S     + P  +GA  +L   V  F
Sbjct:   73 GGTEKIKDPRPLHDKTFVQQCIKQLCEFLGDNGFPGTITVKALQSPSTKEFLKIFEFIYSLLDPTFQLPTTKI--EEEIPRMFKDLGYPFALSKSSMYSIGAPHTWPQALGALIWLIDAVKIF 193
[Close]
BLAST of SMED30014746 vs. Ensembl Cavefish
Match: ndc80 (NDC80 kinetochore complex component [Source:NCBI gene;Acc:103037330])

HSP 1 Score: 54.299 bits (129), Expect = 4.032e-7
Identity = 36/123 (29.27%), Postives = 58/123 (47.15%), Query Frame = 3 
Query:   84 GNTDK-SDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAF 449
            G T+K  DPRPL D  F++   ++L +FL    +P  +        +  +   IFE   SL+   F+L  ++I  E++I R F  + Y    SK+ + S     + P  +GA  +L   V  F
Sbjct:   73 GGTEKIKDPRPLHDKTFVQQCIKQLCEFLGDNGFPGTITVKALQSPSTKEFLKIFEFIYSLLDPTFQLPTTKI--EEEIPRMFKDLGYPFALSKSSMYSIGAPHTWPQALGALIWLIDAVKIF 193
[Close]
BLAST of SMED30014746 vs. Planmine SMEST
Match: SMESG000040783.1 (SMESG000040783.1)

HSP 1 Score: 1082.01 bits (2797), Expect = 0.000e+0
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 3 
Query:   27 MKKSLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLFPRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKSTSEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSISKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVDIQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNAFPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQLQELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESDEHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYESDIDFNQECADIQLQILQESHDFRESSMKRNAVI 1625
            MKKSLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLFPRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKSTSEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSISKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVDIQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNAFPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQLQELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESDEHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYESDIDFNQECADIQLQILQESHDFRESSMKRNAVI
Sbjct:    1 MKKSLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLFPRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKSTSEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSISKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVDIQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNAFPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQLQELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESDEHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYESDIDFNQECADIQLQILQESHDFRESSMKRNAVI 533
[Close]
BLAST of SMED30014746 vs. Planmine SMEST
Match: SMESG000040783.1 (SMESG000040783.1)

HSP 1 Score: 1075.08 bits (2779), Expect = 0.000e+0
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 3 
Query:   36 SLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLFPRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKSTSEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSISKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVDIQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNAFPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQLQELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESDEHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYESDIDFNQECADIQLQILQESHDFRESSMKRNAVI 1625
            SLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLFPRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKSTSEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSISKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVDIQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNAFPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQLQELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESDEHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYESDIDFNQECADIQLQILQESHDFRESSMKRNAVI
Sbjct:   84 SLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLESTDYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRFFTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLFPRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKSTSEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSISKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVDIQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNAFPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQLQELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESDEHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYESDIDFNQECADIQLQILQESHDFRESSMKRNAVI 613
The following BLAST results are available for this feature:
BLAST of SMED30014746 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
NDC807.563e-624.77NDC80 kinetochore complex component [Source:HGNC S... [more]
back to top
BLAST of SMED30014746 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4V3SEE91.051e-826.57Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4Z2DHV11.544e-729.93Kinetochore protein NDC80 isoform 1 OS=Schistosoma... [more]
A0A4Z2DH211.646e-729.93Kinetochore protein NDC80 isoform 3 OS=Schistosoma... [more]
A0A183R3S52.022e-734.41Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A5J4NKL52.441e-731.68Kinetochore protein NDC80 OS=Paragonimus westerman... [more]
back to top
BLAST of SMED30014746 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ndc804.022e-729.27NDC80 kinetochore complex component [Source:NCBI g... [more]
ndc804.032e-729.27NDC80 kinetochore complex component [Source:NCBI g... [more]
back to top
BLAST of SMED30014746 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30014746 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000040783.10.000e+0100.00SMESG000040783.1[more]
SMESG000040783.10.000e+0100.00SMESG000040783.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014746 ID=SMED30014746|Name=SMED30014746|organism=Schmidtea mediterranea sexual|type=transcript|length=2244bp
TATTACTGTATTTGAAAATTTAGCAAATGAAAAAGTCATTAGCCCCGAAA
AAAGATCCCATCTCAAATACAAATGCTGGAAGAGGCAACACAGATAAATC
GGACCCGAGACCTTTAAGAGATCCAAATTTTATGAAAGATGTAAAGCAAA
AATTAATTAAATTTTTAGAAAGCACTGATTACCCTCACCGAGTCGACAGC
TCTATTTTTGGTAAACTTACACAAACAGATGTGAAAGCAATTTTCGAACA
TTTTGTCAGTCTTGTTGTTTTAGATTTTCGTTTGGATGACTCACAAATTC
CTGCAGAAGATCAAATTTTAAGGTTTTTTACTTCCATCCAATATAACAAT
AATTTTTCCAAAACTCACATAACATCGCCAGCAATCTTCTCCTCGGTTCC
ATTCGTTATTGGAGCGTTTGATTTTTTGAGACGATTTGTCAGCGCTTTTA
TTCGAACCGATCCTAAAGTGCTTTTTCCGAGAGTTACAAGATATGGCATC
AGCTCAGAAGTCGTTTTGGATTCTTTGATTTCTATTTATCGTACCATTGA
AAACGCTGAATTCAACACAAACAATCTAATTGAGCTGCAGGATGCCATTC
GAAATGCAATCGGCATTAAATCTACCAGTGAAATTATGAAAATCTCCCAT
AATGTCGACGAAATTGAACAGATGCAAACGAATGTTCAACGACTTGAACG
CACCATCCAAGAATTATCCGAGGAATCCAATCGGATTGCCATTTGCATTG
AAACCGAGGATGAAAAACATTCGATATCGAAAGAAATCCTCCACAAACTA
GAAATAGAAATCCGAGATGAAACTAACTGTTCCAATGCGATTGCGAATGA
GATTTCGCTTCTAGTTGCCAAAAAGAGAGAATACGAGGAAAAAGGGTACT
CAGACACAATCAATCAGATCGTGGATATACAGCATAAATTAACTAGAACG
CGAGCACAATCGCAGAAATACAAATTGGAATCGGAAGAATTAAAACAAAC
GATCCGATCGATGGAGCTCGAGATTGATGATGTGAAATTCCAGTTGCAGA
AAATATTTGCTGATGCCTACAACGCTTTCCCAGACTTAGTCAATGAAGAA
ATTTGTCAACAAATCACGAATTTCAGCCCCGAATATCTACTGAATTACGA
CGGTCAATTGCGACCATATATATCAAACTTGATAGCTGTCATCGAAAGCA
AAGCTCTTATCAGACGAGAGCAATTACAGGAACTCGTCAAAGAAAGCGAA
GATTTAAAGAGGAATTCTGAAGCTTTGAAAAGTGAAATAAAGCTGTTGGA
ATCTAAGAATCAAATAGCTATCAACAATTTCAATCAAAGTGAAGCGATTT
GGTTGACATTACAAGAGGAATCTGACGAACATTGTAAAATCATTCAGAAT
TTAACGGATCAGATTAATCACTATCTCAACGATAATGATTACATTAATCA
GCAACTGAGCGAGATAAGAAAGAGATTTGACGAGGATAAAAGTACATTTG
AGGAAGCAAGCCAGTTCTATGAAAGCGATATCGATTTCAATCAAGAATGC
GCTGATATACAACTGCAGATTCTCCAGGAAAGTCATGATTTTAGAGAATC
ATCAATGAAACGGAATGCAGTAATCTTTGAGGAAAAAAAAGAAAATAGTA
AATCATTTGAAGCTAACCTAGAAAAGCGCAAGAAAGAACTGATGAAAAAG
TTAACCGAAGAACTGGCAATAATTGATGAAAATTTCAATTTTTGATTTTG
TATATTTTTTATTATTTTTGTTTGGTTATTTTTTATGTTTATTATAATTT
ATTATTCATTTATATACATCCTATCTTTTATGGGATTTCAGATTATTAAA
GAACTGAACTGAAATTTTTGTATTTTCGGTGAAAAAAAATCGCATAAATG
TGAATAAAAAGAAAAGTGTACCTACGAAAAAAATCGCGTATAAAAAGGAA
AGTGTAAATTATTGTTGGTTTTGTCATTTTATTAATTATTTGATTACTAA
TAATCTAAAGATTTTTTTAAAATTGTGATGTGGACATAAGCAGGAATCTA
ACATAATAGAGCAAAATTATTAATATTGGATTATTATAAATTGGGCAAGA
TTTTTTATATAGCTTTCTTCATACTAGTCTTTAATCCCGGTACAATAAGG
TGCGTGAGTTAAGGGCTTGATGTATAATTTTCTTGTTGAGCAACATGATA
GGTAAACGAATTAGGGAAGATCAATAATGCACGGAAAGGCATCG
back to top

protein sequence of SMED30014746-orf-1

>SMED30014746-orf-1 ID=SMED30014746-orf-1|Name=SMED30014746-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=573bp
MKKSLAPKKDPISNTNAGRGNTDKSDPRPLRDPNFMKDVKQKLIKFLEST
DYPHRVDSSIFGKLTQTDVKAIFEHFVSLVVLDFRLDDSQIPAEDQILRF
FTSIQYNNNFSKTHITSPAIFSSVPFVIGAFDFLRRFVSAFIRTDPKVLF
PRVTRYGISSEVVLDSLISIYRTIENAEFNTNNLIELQDAIRNAIGIKST
SEIMKISHNVDEIEQMQTNVQRLERTIQELSEESNRIAICIETEDEKHSI
SKEILHKLEIEIRDETNCSNAIANEISLLVAKKREYEEKGYSDTINQIVD
IQHKLTRTRAQSQKYKLESEELKQTIRSMELEIDDVKFQLQKIFADAYNA
FPDLVNEEICQQITNFSPEYLLNYDGQLRPYISNLIAVIESKALIRREQL
QELVKESEDLKRNSEALKSEIKLLESKNQIAINNFNQSEAIWLTLQEESD
EHCKIIQNLTDQINHYLNDNDYINQQLSEIRKRFDEDKSTFEEASQFYES
DIDFNQECADIQLQILQESHDFRESSMKRNAVIFEEKKENSKSFEANLEK
RKKELMKKLTEELAIIDENFNF*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR005550Kinetochore_Ndc80
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 331..366
NoneNo IPR availableCOILSCoilCoilcoord: 194..231
NoneNo IPR availableCOILSCoilCoilcoord: 3..30
NoneNo IPR availableCOILSCoilCoilcoord: 102..136