Usp domain-containing protein

Overview
NameUsp domain-containing protein
Smed IDSMED30014689
Length (bp)595
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Usp domain-containing protein (SMED30014689) t-SNE clustered cells

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30014689) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Usp domain-containing protein (SMED30014689) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Usp domain-containing protein (SMED30014689) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014689

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30014689h1SMcG0015161 Contig44355newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014689h1SMcG0015161 Contig44355uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30014689h1SMcG0015161 dd_Smed_v4_985_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014689h1SMcG0015161 dd_Smed_v4_985_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014689h1SMcG0015161 dd_Smed_v4_985_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014689h1SMcG0015161 dd_Smed_v4_985_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30014689h1SMcG0015161 dd_Smed_v6_985_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|A0QZA1|Y3950_MYCS2 (Universal stress protein MSMEG_3950/MSMEI_3859 OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_3950 PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 5.928e-7
Identity = 37/146 (25.34%), Postives = 65/146 (44.52%), Query Frame = 3 
Query:   96 ILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVII 533
            IL+ VD S  ++ A+ W        +  + ++H++ P+  S   G  M       E  E   +  +++ + +V      L E  G      I    V   +I    + +  +++V NRG G + R  LGS S  +LH+A  PVV++
Sbjct:   10 ILVGVDSSAESDAAVRWAAREASLHDAPITLMHVIAPVVVSWPAGPYMATVL---ECQEENARHAIEQAQKVVADC---LGETHGLTVQTEIRKESVARTLIDASKSAQ--MVVVGNRGMGALGRVLLGSTSTSLLHYASGPVVVV 147
[Close]
BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Match: sp|Q57997|Y577_METJA (Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0577 PE=1 SV=1)

HSP 1 Score: 49.2914 bits (116), Expect = 2.230e-6
Identity = 40/155 (25.81%), Postives = 83/155 (53.55%), Query Frame = 3 
Query:   96 ILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEP--MYTSTAVGIGMEVAFL--PVEDLESKIKKQL-DKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 545
            IL P D SE  E A+           + V ++H+++   +       + + VA L   VE+ E+++K +L ++ K+ ++    EL++ G + K  ++V     E I+K+     VD+II+ + G+  ++   LGSV++ V+  +++PV+++  K+
Sbjct:    8 ILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIP-HEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKN 161
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A183WUS6 (Usp domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS15878 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 2.893e-48
Identity = 78/161 (48.45%), Postives = 112/161 (69.57%), Query Frame = 3 
Query:   60 MSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPK 542
            MS+ N  E  RV+LMP+D SEH +RA  WY+EN+ +PND+V+ I+IVEP+Y + AVG+ ME    P+ D+   ++  +D GK L KK+  E ++ G   + ++ VD++ G A++K    H  ++II+ NRG G IRRTFLGSVS+YVLHHA  PV+I+PPK
Sbjct:    1 MSEVN--ETPRVVLMPIDGSEHAQRAFQWYIENMKRPNDIVKFINIVEPVYATPAVGLTMETP--PLTDITKMMQDSIDNGKVLGKKYITEAKQCGVNCQAFLHVDTRPGAALLKSIEDHNANVIIMGNRGLGAIRRTFLGSVSEYVLHHAHIPVIIVPPK 157
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A448X5I9 (Usp domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS22031 PE=4 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 7.592e-46
Identity = 77/178 (43.26%), Postives = 119/178 (66.85%), Query Frame = 3 
Query:    6 IACFGIY*STFRYHFIIAMSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPP 539
            + C  ++        + AMS+S    + R IL+P+D SEH++RA  WY+ENL K  D V +IH++EP+YT+ AVG+ ME   L V+D+   +++ ++ GK L ++F  + +E G  +K ++ VD+K G A++K    HK D+I++ NRG G +RRTFLGSVSD+VLHH+  PVV++PP
Sbjct:    6 VCCLTLFQPGPLSSRLSAMSESKG-AILRNILIPIDGSEHSDRAFVWYIENLKKAGDCVHLIHVIEPVYTTPAVGLAMESPPLLVDDMTRVMEESIESGKKLGQQFMRKAKEFGVDSKAFLHVDTKPGHALVKSSIDHKADIIVMGNRGLGTLRRTFLGSVSDHVLHHSHIPVVVVPP 182
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A183QK93 (Usp domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 2.609e-45
Identity = 74/158 (46.84%), Postives = 107/158 (67.72%), Query Frame = 3 
Query:   69 SNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPK 542
            S +    RV+L+P+D SEH ERA  WY+ N+   ND+V  ++IVEP+Y + AVG+ ME    P+ D+   ++  +DKGK L KK+  E ++ G   + ++ VD++ G A++K    H  +LII+ +RG G++RRTFLGSVSDYVLHHA  PVVI+PPK
Sbjct:    2 SESSAKSRVVLLPIDGSEHAERAFHWYLNNMRSSNDIVTFVNIVEPVYATPAVGLTMETP--PLTDITKIMQDSIDKGKLLGKKYIAEAKKYGINCQAFIHVDNRPGAALLKSIEDHNANLIIMGSRGLGILRRTFLGSVSDYVLHHAHIPVVIVPPK 157
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A3Q0KV25 (Smp_202690 OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 6.954e-45
Identity = 74/158 (46.84%), Postives = 108/158 (68.35%), Query Frame = 3 
Query:   69 SNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPK 542
            S + E  RV+L+P+D SEH ERA  WY+ N+   ND+V+ ++IVEP+Y + AVG+ ME    P+ D+   ++  +DKGK L KK+  E ++     + ++ VD++ G A++K    H  +LII+ +RG G++RRTFLGSVSDYVLHHA  PVVI+PPK
Sbjct:    2 SGSTEKSRVVLLPIDGSEHAERAFQWYLTNMKSSNDIVKFVNIVEPVYATPAVGLTMETP--PLTDITKIMQDSIDKGKLLGKKYIAEAKKYDINCQAFLHVDNRPGAALLKSIEDHNANLIIMGSRGLGILRRTFLGSVSDYVLHHAHIPVVIVPPK 157
[Close]
BLAST of Usp domain-containing protein vs. TrEMBL
Match: A0A419Q819 (Universal stress protein A-like protein OS=Clonorchis sinensis OX=79923 GN=CSKR_2815s PE=4 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 7.858e-45
Identity = 72/162 (44.44%), Postives = 109/162 (67.28%), Query Frame = 3 
Query:   57 AMSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPK 542
            AM+ +  +E  R I++PVD SEH+ERA  WY+ N+M+PND V+ ++I+EP+YTS   G  +E+  LP  D+   + + ++ GK L ++  H+ +     ++ ++ VDS+ G AI+K    +  DL+I+ NRG G +RRTFLGSVSDYVLHH+  PVVI+PP 
Sbjct:   18 AMASARVNEKPRTIVLPVDGSEHSERAFRWYLNNVMQPNDNVKFVNIIEPVYTSPGFGAAIELPSLP--DVSRVMAETVEAGKKLCQEKMHQAKAYNINSQAFLHVDSRPGPAIVKAVQDYNADLVIMGNRGIGTVRRTFLGSVSDYVLHHSHAPVVIVPPN 177
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO33143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST34])

HSP 1 Score: 103.605 bits (257), Expect = 1.885e-28
Identity = 54/149 (36.24%), Postives = 86/149 (57.72%), Query Frame = 3 
Query:   90 RVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIP 536
            R IL+PVD SEH+ERA DWY E L  P D V ++H +E       +       F   E+  + +K+  ++ +++++ +    +EK    +  ++V    G+ I +V      +LI++  RG+G+IRRT LGSVSDYV+HH+  PV +IP
Sbjct:    4 RKILIPVDGSEHSERAFDWYAELLHSPGDEVLVVHCIE--LPPVPLEHQFPFVFAYYEEWSAMVKETREQHEAMLRSYEDICKEKKLHYEIMMVVGKPAGDVICQVARDVSANLIVLGTRGQGMIRRTILGSVSDYVVHHSHLPVAVIP 150
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO45800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRQ2])

HSP 1 Score: 98.5969 bits (244), Expect = 1.497e-26
Identity = 56/151 (37.09%), Postives = 89/151 (58.94%), Query Frame = 3 
Query:   90 RVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPK 542
            R IL+P+D S+H+E A +WYV N+    D + ++H+++    S A+   +    L  ++ ++++ K L + K+L +K+  + +    +AK   +   K GE I +       DLI++ +RG G IRRT LGSVSDYVLHHA  P +IIP K
Sbjct:   10 RRILLPIDSSKHSEDAFEWYVNNMHHEEDELILVHVLD----SAAIQTRVSSHGLVDDEFKNEMNKGLKEVKALEEKYKTKAETASLKAK-IEVRGGKPGETICQCSKDEHCDLILMGSRGLGSIRRTILGSVSDYVLHHAHVPTIIIPKK 155
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO49708 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFF6])

HSP 1 Score: 95.1301 bits (235), Expect = 4.585e-25
Identity = 50/162 (30.86%), Postives = 92/162 (56.79%), Query Frame = 3 
Query:   63 SDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVE--PMY-TSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPP 539
            S  +    +R +L+ +D SEH+ RA +WY EN+ + ++L+ ++H  E  P++    A G  +   +L      ++ KK   + K L++ F    +E+    + +++     G AIIK+    K + +++ +RG+ ++RRT +GSVSD+++HHA  PV I PP
Sbjct:    5 SQQDTKYEKRRVLLAIDHSEHSMRAFEWYFENIHRDDNLLMLVHSQELPPIFIPPDAFGTTLYNEWL------AEAKKASLQSKKLLEGFERMCKERHCECEKHLLEGDNPGPAIIKLIKKSKPNYVVIGSRGQSMVRRTVMGSVSDFIIHHAHVPVCISPP 160
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO44746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUS8])

HSP 1 Score: 93.2041 bits (230), Expect = 2.141e-24
Identity = 51/152 (33.55%), Postives = 88/152 (57.89%), Query Frame = 3 
Query:   87 ERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVE-PMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPP 539
            +R +++PVD S+H+ERA +WY +++ +P D V +IH  E P   S+    G  V  LP ++    + + +   K L++++  + +E+G   + +     + GE I ++        +++ +RG G IRRT LGSVSDY +HH+  PV +IPP
Sbjct:    6 KRRVVIPVDGSQHSERAFNWYRQHVHEPGDEVLIIHTQEQPTIPSSPYAYGGTV--LP-DEWNKAVDECIVNAKKLIEEYNKKCKEQGMTCRLFK-GSGQPGETICQLAKDLSAKHVVMGSRGCGTIRRTLLGSVSDYCVHHSSVPVTVIPP 153
[Close]
BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Match: EDO42478 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1B3])

HSP 1 Score: 93.2041 bits (230), Expect = 2.756e-24
Identity = 58/162 (35.80%), Postives = 86/162 (53.09%), Query Frame = 3 
Query:   93 VILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVE--PMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKF-----------THELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPP 539
            V+L+PVD S+++ RA DWY ++  + ND V ++   E  PM  +    +  +   L  + L  K +   DK +S++K F           ++ L   GG+A          GE II +     VD II+ +RG G  RRT LGSVSDYV+HHA  PV+++PP
Sbjct:   10 VVLIPVDGSKNSIRAFDWYKDHYHQENDKVLIVSAYEIPPMQAAKHASVDFKNQLLEWQILRQKAE---DKARSILKVFEQRCLPFKELISYRLLPGGGKA----------GEVIIGIAKQENVDEIIIGSRGLGKFRRTILGSVSDYVVHHASVPVIVVPP 158
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020952.1 (SMESG000020952.1)

HSP 1 Score: 330.872 bits (847), Expect = 1.539e-117
Identity = 162/162 (100.00%), Postives = 162/162 (100.00%), Query Frame = 3 
Query:   60 MSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 545
            MSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH
Sbjct:    1 MSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 162
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020955.1 (SMESG000020955.1)

HSP 1 Score: 232.261 bits (591), Expect = 1.305e-78
Identity = 103/158 (65.19%), Postives = 136/158 (86.08%), Query Frame = 3 
Query:   75 NDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPV-EDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 545
            +DE ERVI++P+D SE+TER +DWYVEN+MKP+D+V M++IVEP++ S + G+ ME A L + ED E+KIKKQ+DKGK LVK++ HEL++KGG+ KGY+ VDS VGEAI+KVCH HK D+++++NRGEG+IRRTFLGSVSDYVLHH DRPV+I+PP H
Sbjct:    7 DDETERVIVIPIDNSEYTERTVDWYVENIMKPHDMVHMVNIVEPIFNSFSAGLAMEAAILTMSEDAETKIKKQIDKGKQLVKRYIHELRDKGGKGKGYIRVDSNVGEAIVKVCHEHKADIVLLSNRGEGIIRRTFLGSVSDYVLHHVDRPVIIVPPLH 164
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020882.1 (SMESG000020882.1)

HSP 1 Score: 225.328 bits (573), Expect = 1.037e-75
Identity = 98/155 (63.23%), Postives = 133/155 (85.81%), Query Frame = 3 
Query:   81 EVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 545
            E ER+I++P+D+SE + RA+DWY  N+MK ND+V +IHIVEP+Y + AVGIGME A+LP+E+L++KI+KQ+DKGK+LV+++   L +KGG+ KG++ VDS+VGE II VCH H  D+I++ANRGEGL+RRTFLGSVSDYVLHHAD+PV+I+PP H
Sbjct:   16 ESERIIIIPIDKSEVSRRALDWYSNNMMKENDVVHLIHIVEPIYNTIAVGIGMEAAWLPMEELDAKIQKQIDKGKALVREYISNLTDKGGKCKGFIRVDSRVGECIISVCHDHLADVILMANRGEGLLRRTFLGSVSDYVLHHADKPVIIVPPLH 170
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000020884.1 (SMESG000020884.1)

HSP 1 Score: 189.119 bits (479), Expect = 5.569e-62
Identity = 83/129 (64.34%), Postives = 112/129 (86.82%), Query Frame = 3 
Query:  159 LMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPKH 545
            +M+ ND+V +IHIVEP+Y + AVGIGME A+LP+E+L++KI+KQ+DKGK+LV+++   L +KGG+ KG++ VDS+VGE II VCH H  D+I++ANRGEGL+RRTFLGSVSDYVLHHAD+PV+I+PP H
Sbjct:    1 MMRENDVVHLIHIVEPIYNTIAVGIGMEAAWLPMEELDAKIQKQIDKGKALVREYISNLTDKGGKCKGFIRVDSRVGECIISVCHDHLADVILMANRGEGLLRRTFLGSVSDYVLHHADKPVIIVPPLH 129
[Close]
BLAST of Usp domain-containing protein vs. Planmine SMEST
Match: SMESG000016283.1 (SMESG000016283.1)

HSP 1 Score: 157.147 bits (396), Expect = 5.367e-49
Identity = 70/153 (45.75%), Postives = 111/153 (72.55%), Query Frame = 3 
Query:   84 VERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHADRPVVIIPPK 542
             +R I++P+D SEH++RA  WY++N+ +PNDLV  IH+VEP+Y + AVG+ ME   + ++D+   ++  +++GK L ++F HE ++ G   K ++ VD+K G A++K    H+ D+II+ NRG G +RRTFLGSVSD++LHHA  PVV++PP+
Sbjct:    2 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHIPVVVVPPE 153
The following BLAST results are available for this feature:
BLAST of Usp domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|A0QZA1|Y3950_MYCS25.928e-725.34Universal stress protein MSMEG_3950/MSMEI_3859 OS=... [more]
sp|Q57997|Y577_METJA2.230e-625.81Universal stress protein MJ0577 OS=Methanocaldococ... [more]
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BLAST of Usp domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183WUS62.893e-4848.45Usp domain-containing protein OS=Trichobilharzia r... [more]
A0A448X5I97.592e-4643.26Usp domain-containing protein OS=Protopolystoma xe... [more]
A0A183QK932.609e-4546.84Usp domain-containing protein OS=Schistosoma rodha... [more]
A0A3Q0KV256.954e-4546.84Smp_202690 OS=Schistosoma mansoni OX=6183 PE=4 SV=... [more]
A0A419Q8197.858e-4544.44Universal stress protein A-like protein OS=Clonorc... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO331431.885e-2836.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO458001.497e-2637.09Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO497084.585e-2530.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447462.141e-2433.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424782.756e-2435.80Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Usp domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Usp domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020952.11.539e-117100.00SMESG000020952.1[more]
SMESG000020955.11.305e-7865.19SMESG000020955.1[more]
SMESG000020882.11.037e-7563.23SMESG000020882.1[more]
SMESG000020884.15.569e-6264.34SMESG000020884.1[more]
SMESG000016283.15.367e-4945.75SMESG000016283.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014689 ID=SMED30014689|Name=Usp domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=595bp
GTTCAATTGCATGTTTCGGAATATATTAATCTACATTCAGATATCATTTC
ATAATTGCAATGTCAGATAGCAACAACGACGAGGTCGAAAGAGTGATCTT
GATGCCAGTAGACCGAAGCGAACATACCGAAAGAGCGATCGACTGGTACG
TGGAAAATCTAATGAAACCCAATGATTTGGTTCAAATGATTCATATTGTC
GAACCAATGTATACATCAACAGCTGTCGGAATCGGAATGGAAGTAGCTTT
CTTGCCAGTTGAAGACCTTGAAAGTAAAATAAAAAAACAATTAGACAAAG
GGAAATCGTTGGTTAAGAAGTTTACTCATGAATTACAAGAGAAAGGTGGT
CGAGCAAAAGGTTATGTGATTGTCGACTCGAAAGTGGGGGAGGCTATAAT
AAAAGTGTGCCACACTCATAAAGTGGATTTAATTATAGTTGCCAATCGAG
GAGAAGGACTAATCCGAAGGACATTCCTTGGAAGCGTCAGTGATTATGTC
CTCCATCATGCCGACAGACCTGTCGTAATCATACCACCTAAACATTAATA
CATGTTAATTAACTTTAATAAAATTTTCAAATATTTTTAAAAAAA
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protein sequence of SMED30014689-orf-1

>SMED30014689-orf-1 ID=SMED30014689-orf-1|Name=SMED30014689-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=163bp
MSDSNNDEVERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPM
YTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAK
GYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHH
ADRPVVIIPPKH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000421tail
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR006015Universal_stress_UspA
IPR014729Rossmann-like_a/b/a_fold
IPR006016UspA
Vocabulary: biological process
TermDefinition
GO:0006950response to stress
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 137..159
score: 38.09
coord: 119..131
score: 32.31
coord: 10..28
score: 30.74
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 5..162
e-value: 2.1E-32
score: 114.7
IPR006016UspAPFAMPF00582Uspcoord: 11..159
e-value: 4.7E-24
score: 85.5
NoneNo IPR availablePANTHERPTHR46989FAMILY NOT NAMEDcoord: 11..161
NoneNo IPR availableCDDcd00293USP_Likecoord: 13..158
e-value: 6.4507E-22
score: 83.5734
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 13..161