Chromatin licensing and DNA replication factor 1

Overview
NameChromatin licensing and DNA replication factor 1
Smed IDSMED30014686
Length (bp)2152
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Chromatin licensing and DNA replication factor 1 (SMED30014686) t-SNE clustered cells

Violin plots show distribution of expression levels for Chromatin licensing and DNA replication factor 1 (SMED30014686) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Chromatin licensing and DNA replication factor 1 (SMED30014686) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Chromatin licensing and DNA replication factor 1 (SMED30014686) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014686

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30014686h1SMcG0020844 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30014686h1SMcG0020844 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30014686h1SMcG0020845 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30014686h1SMcG0020845 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30014686h1SMcG0020844 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30014686h1SMcG0020844 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30014686h1SMcG0020845 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30014686h1SMcG0020845 SmedASXL_014210SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
parenchymal cellSMED30014686h1SMcG0020845 dd_Smed_v4_11432_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014686h1SMcG0020844 dd_Smed_v4_11432_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014686h1SMcG0020845 dd_Smed_v4_11432_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014686h1SMcG0020844 dd_Smed_v4_11432_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014686h1SMcG0020845 dd_Smed_v6_19937_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30014686h1SMcG0020845 dd_Smed_v6_19937_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Human
Match: CDT1 (chromatin licensing and DNA replication factor 1 [Source:HGNC Symbol;Acc:HGNC:24576])

HSP 1 Score: 59.6918 bits (143), Expect = 1.319e-8
Identity = 44/180 (24.44%), Postives = 86/180 (47.78%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPV-NSPFSESHLGKIITIYHEAYLIKWER-LPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELI--NDYLLNESWHCKFNIDLHVELVDPTPMPGRP 1589
            L +  + L E+F  +D +V  ++ + E    +++   +  +    F E ++G+I T+Y  +Y  + ER +P+           +TI+P L  +         D    +    +L +RR IF ++ +EHV   ++ FL +  ++P ++   D L    WH +FN+D  V  ++P  +P  P
Sbjct:  186 LPYKYQVLAEMFRSMDTIVGMLHNRSETPTFAKVQRGVQDMMRRRFEECNVGQIKTVYPASYRFRQERSVPTFKDGTRRSDYQLTIEPLLEQEA--------DGAAPQLTASRLLQRRQIFSQKLVEHVKEHHKAFLAS--LSPAMVVPEDQLTR--WHPRFNVD-EVPDIEPAALPQPP 352
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Fly
Match: dup (gene:FBgn0000996 transcript:FBtr0087387)

HSP 1 Score: 56.6102 bits (135), Expect = 6.804e-8
Identity = 63/234 (26.92%), Postives = 102/234 (43.59%), Query Frame = 3 
Query:  297 NSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGM-NHDFNERIFRQILNIFPESYFYE------FNSTPIKLAKYFREQLKLVPNLSFGPAVDCS--PRKLEK-----CLSVAQ--------ITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLI---KVQEWHCKFDLENCVE-EIQPVFLDRNF-KYPIKQTIIKKPESEKVFRNIFKVPT 917
            +S   ++PLP  ++ LLD+F  LD  V    +  +++   ++   ++ M   +F E    QI +I+P+++ +       F S  +  A YF  QL + PN+   P    S  P+   K      L+ AQ        +T R Q  Q+ LL    + HD FL          +  +I    +  WH +FDLE+C E E+ P+    N  KY   + I+         RN+F   T
Sbjct:  310 SSRAGQLPLPYKYRHLLDVFKGLDSVVAMFHNRKETITFKKLKPAVQRMLRKNFTETHLAQIKHIYPDAFIFSQVKTRNFGS--VSKADYF--QLIIAPNVEPLPEQQQSEKPQHFTKINEDDVLASAQSTSMNPHVMTARMQRFQSLLLDRAMRAHDQFLR-------SQDPPIIIEKALTRWHPQFDLESCPEVELSPLPQPPNVEKYSSAKDIL------STARNLFNCAT 526
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Fly
Match: dup (gene:FBgn0000996 transcript:FBtr0339999)

HSP 1 Score: 56.6102 bits (135), Expect = 6.804e-8
Identity = 63/234 (26.92%), Postives = 102/234 (43.59%), Query Frame = 3 
Query:  297 NSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGM-NHDFNERIFRQILNIFPESYFYE------FNSTPIKLAKYFREQLKLVPNLSFGPAVDCS--PRKLEK-----CLSVAQ--------ITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLI---KVQEWHCKFDLENCVE-EIQPVFLDRNF-KYPIKQTIIKKPESEKVFRNIFKVPT 917
            +S   ++PLP  ++ LLD+F  LD  V    +  +++   ++   ++ M   +F E    QI +I+P+++ +       F S  +  A YF  QL + PN+   P    S  P+   K      L+ AQ        +T R Q  Q+ LL    + HD FL          +  +I    +  WH +FDLE+C E E+ P+    N  KY   + I+         RN+F   T
Sbjct:  310 SSRAGQLPLPYKYRHLLDVFKGLDSVVAMFHNRKETITFKKLKPAVQRMLRKNFTETHLAQIKHIYPDAFIFSQVKTRNFGS--VSKADYF--QLIIAPNVEPLPEQQQSEKPQHFTKINEDDVLASAQSTSMNPHVMTARMQRFQSLLLDRAMRAHDQFLR-------SQDPPIIIEKALTRWHPQFDLESCPEVELSPLPQPPNVEKYSSAKDIL------STARNLFNCAT 526
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Xenopus
Match: CDT1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:100379812])

HSP 1 Score: 52.7582 bits (125), Expect = 1.771e-6
Identity = 56/219 (25.57%), Postives = 99/219 (45.21%), Query Frame = 3 
Query:  321 LPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKG-MNHDFNERIFRQILNIFPESYFY-EFNSTP-----IKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLENCVEEIQPVFL---DRNFKYPIKQTIIKK------PESEKVFRNI-FKVPTNSG 926
            LP  +KVL ++F S+D  V  L +  +++  +++ + ++  M   F +R   QI  ++P +Y Y +  + P     +K   Y   QL + P +  G  ++  P      LS + +  R+Q     L  IVKQ H +FL     +N        K+  WH +F+++  V ++ P  L    +  K    Q ++ K      P+ EK   N+  K   N+G
Sbjct:  211 LPYKYKVLAEMFRSMDTIVGMLFNRSETITFSKVKQGVQDMMRKQFEQRNVGQIKTVYPTAYKYRQEKNIPTFKDGVKKTDY---QLTIDPLIDEGDKLNGRPH-----LSASSLLQRRQLFHRSLTSIVKQHHKVFL---ASLNPPMVVPDDKLTRWHPRFNVDE-VPDVMPSELPLPPQVEKLTTAQEVLSKARGMITPKMEKALANLALKTAENAG 417
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Xenopus
Match: CDT1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:100379812])

HSP 1 Score: 52.7582 bits (125), Expect = 2.045e-6
Identity = 56/219 (25.57%), Postives = 99/219 (45.21%), Query Frame = 3 
Query:  321 LPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKG-MNHDFNERIFRQILNIFPESYFY-EFNSTP-----IKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLENCVEEIQPVFL---DRNFKYPIKQTIIKK------PESEKVFRNI-FKVPTNSG 926
            LP  +KVL ++F S+D  V  L +  +++  +++ + ++  M   F +R   QI  ++P +Y Y +  + P     +K   Y   QL + P +  G  ++  P      LS + +  R+Q     L  IVKQ H +FL     +N        K+  WH +F+++  V ++ P  L    +  K    Q ++ K      P+ EK   N+  K   N+G
Sbjct:  258 LPYKYKVLAEMFRSMDTIVGMLFNRSETITFSKVKQGVQDMMRKQFEQRNVGQIKTVYPTAYKYRQEKNIPTFKDGVKKTDY---QLTIDPLIDEGDKLNGRPH-----LSASSLLQRRQLFHRSLTSIVKQHHKVFL---ASLNPPMVVPDDKLTRWHPRFNVDE-VPDVMPSELPLPPQVEKLTTAQEVLSKARGMITPKMEKALANLALKTAENAG 464
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. UniProt/SwissProt
Match: sp|Q9H211|CDT1_HUMAN (DNA replication factor Cdt1 OS=Homo sapiens OX=9606 GN=CDT1 PE=1 SV=3)

HSP 1 Score: 59.6918 bits (143), Expect = 6.335e-8
Identity = 44/180 (24.44%), Postives = 86/180 (47.78%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPV-NSPFSESHLGKIITIYHEAYLIKWER-LPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELI--NDYLLNESWHCKFNIDLHVELVDPTPMPGRP 1589
            L +  + L E+F  +D +V  ++ + E    +++   +  +    F E ++G+I T+Y  +Y  + ER +P+           +TI+P L  +         D    +    +L +RR IF ++ +EHV   ++ FL +  ++P ++   D L    WH +FN+D  V  ++P  +P  P
Sbjct:  186 LPYKYQVLAEMFRSMDTIVGMLHNRSETPTFAKVQRGVQDMMRRRFEECNVGQIKTVYPASYRFRQERSVPTFKDGTRRSDYQLTIEPLLEQEA--------DGAAPQLTASRLLQRRQIFSQKLVEHVKEHHKAFLAS--LSPAMVVPEDQLTR--WHPRFNVD-EVPDIEPAALPQPP 352
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. TrEMBL
Match: A0A0X3PKA8 (DNA replication factor Cdt1 (Fragment) OS=Schistocephalus solidus OX=70667 GN=CDT1 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 7.994e-20
Identity = 89/365 (24.38%), Postives = 165/365 (45.21%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSP-VNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPE--LINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYS--------TNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAM-----------------VSKLRDLVALLYTEFKAVQYKPLALSYLSN--IASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQLSIDKSNLSIDDVL 2066
            L  H+ TL E F   D VV  ++ + E+C   ++  A+   V S F+E+ + +  T+Y ++Y  ++++     +  P     + + PNLR+D T+ A  SP KG L F   +L +RR  F +  I  V  ++R FL+NK    E  L  D  +   WH  F +D  V  +  TP+P +P+  +   ++TS S+     + + +F   ++IC R+S        T  + +   +     + K N   +K ++  L  +      AM                 +S L   +  ++ E +A+  KP+ L+ ++     S    + +   +  +  LI L+P W+E++    P L +   +  + DV+
Sbjct:  153 LPSHMHTLLEFFRACDLVVSVLHNRSEVCSFDKVKLAVQELVRSDFTEAIVSQFATVYPDSYSFRYDKQLDKVTKLPTSSYVLILIPNLRSDGTQMARDSPSKGHLVFTGNRLIQRRHHFQKSLIARVRRAHREFLVNKLSISESDLPPDSPIKR-WHPAFPLDTAVPTIPSTPLPPKPDANES--KITSASEAVAAFRSRALFR-EAEICERFSPHPQDAKKTYKSVVPSPSQASGSLTKSNAVALKGISESLLARVREREKAMQFSLLTRSPISEKQRLALSCLPTTITQVWRELRALSGKPVPLATVATRLAQSSETGLSQEAALYRIDELISLMPDWLEKVAWAKPHLRLKDMDRPLKDVI 513
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. TrEMBL
Match: A0A183TKL1 (DNA replication factor Cdt1 OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS17009 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 9.586e-20
Identity = 89/365 (24.38%), Postives = 165/365 (45.21%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSP-VNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPE--LINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYS--------TNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAM-----------------VSKLRDLVALLYTEFKAVQYKPLALSYLSN--IASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQLSIDKSNLSIDDVL 2066
            L  H+ TL E F   D VV  ++ + E+C   ++  A+   V S F+E+ + +  T+Y ++Y  ++++     +  P     + + PNLR+D T+ A  SP KG L F   +L +RR  F +  I  V  ++R FL+NK    E  L  D  +   WH  F +D  V  +  TP+P +P+  +   ++TS S+     + + +F   ++IC R+S        T  + +   +     + K N   +K ++  L  +      AM                 +S L   +  ++ E +A+  KP+ L+ ++     S    + +   +  +  LI L+P W+E++    P L +   +  + DV+
Sbjct:  175 LPSHMHTLLEFFRACDLVVSVLHNRSEVCSFDKVKLAVQELVRSDFTEAIVSQFATVYPDSYSFRYDKQLDKVTKLPTSSYVLILIPNLRSDGTQMARDSPSKGHLVFTGNRLIQRRHHFQKSLIARVRRAHREFLVNKLSISESDLPPDSPIKR-WHPAFPLDTAVPTIPSTPLPPKPDANES--KITSASEAVAAFRSRALFR-EAEICERFSPHPQDAKKTYKSVVPSPSQASGSLTKSNAVALKGISESLLARVREREKAMQFSLLTRSPISEKQRLALSCLPTTITQVWRELRALSGKPVPLATVATRLAQSSETGLSQEAALYRIDELISLMPDWLEKVAWAKPHLRLKDMDRPLKDVI 535
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. TrEMBL
Match: A0A5J4NS12 (Chromatin licensing and DNA replication factor 1 OS=Paragonimus westermani OX=34504 GN=DEA37_0005660 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 4.821e-18
Identity = 90/360 (25.00%), Postives = 156/360 (43.33%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPV-NSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCI--TPELINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFD----CGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAMVS---------------KLRDLVALLYTEFKAVQYKPLALSYLSNIASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQLSIDKSNLSIDDVLSAI 2075
            L    + L ELF   D VV  ++ + E+C   ++  A+  V    F E H+G+ +TIY   YL+++E+     + +P     + + PNLR D T+    SP KG L F   +L +RR  F    + HV  ++R FL  K    +  L ND  L   WH KF ++  V  + PTP+P RP  T++  ++TS     +  + + +F     C +   LR+            P   I   +  K    +P     +  +++A+ +                L+ +   L  +    Q   L +   SN    S+    +   D+L  L +    W+E+L+   P L +   +L I +V++++
Sbjct:  137 LPHDFRVLLELFRSCDTVVSMLHNRSEICGFDKLKPAVQEVVRRNFEEMHVGQFLTIYPMVYLLRFEKQLDKYTRRPSGNYVLVLSPNLRTDGTQIGHDSPSKGHLVFTGTRLIQRRNRFHRLLLGHVFRAHREFLHTKLGIDSTGLPNDTELRR-WHPKFCLESMVPPIQPTPLPIRPSATEE--KITSAKDAVRAFQARALFREADICQNVALLRHD-----------PMPTIP--SPTKASACSPRKPTVQSASSIALFTSSAASVPPSSPSVACSLKGVSETLLAKQLINQNNELLMRKNSNFLLDSIRYYSDVVADHLKVLQEFCSTWLEKLDWASPHLRLKNPDLPIGEVIASV 480
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. TrEMBL
Match: A0A564ZB75 (CDT1 domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS14507 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.061e-17
Identity = 87/368 (23.64%), Postives = 163/368 (44.29%), Query Frame = 3 
Query: 1059 RLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNS-PFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIK-----------PNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNK--CITPELINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMY---------------KIMKKKN---VFDCGSKICLRYSTNS---------ANIDCKALPQSMIDKVNDNKMKRLAPDL--HVKKGNNNVAMVSKLRDLVALLYTEFKAVQYKPLALSYLSN--IASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQL 2027
            RL FH++ LFELF   + ++  ++ + E+C   +I  A+  V+   F++  +G+ + +Y  AY  +++           K+ID   K           PNLR D T+ A  SP KG L F   +L +RR IF    +  V  ++++FL+++      +L +D  L   WH  F +D  V  V+P  +P  P +  D K +++   +                KI   K         SK  L  S +S         +N++ K +  S++ KV D + K+++  L   +    +  A  + L   +  +++  +    +P+ ++ +++  + S    +        L  L+ L+P W+E++E   P L
Sbjct:  212 RLPFHMERLFELFRTCETIISTLHNRSEICSFDKIKPAVQEVSRCDFTDKTVGQFVALYPSAYSFRYD-----------KQIDRITKLQLCSYTLVFIPNLRTDGTQMATDSPMKGHLIFTGTRLIQRRHIFYNALLNRVKDAHKKFLISRFNLSESDLPDDTGLRR-WHPAFALDTAVPEVEPGALPPHPSDGSDTKIISARDAVVAFRARALFREAKVCEKIAAAKGELPATTSRSKSGLEDSPDSGVSSPVKKPSNVELKGISASLLAKVRD-REKQMSLTLLRQLAATPSEKAAFAALPATITQIWSALRGGSGRPVPITQVTSRLVQSSGSGLSPEEANSRLENLLTLMPNWVEKVEWARPHL 566
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. TrEMBL
Match: A0A0R3U8Z6 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS3383 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.497e-17
Identity = 63/223 (28.25%), Postives = 108/223 (48.43%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPV-NSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPE--LINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDC 1721
            L +H++ L ELF   + +V  ++ + E+C   +I  A+  V    F+E  +GK   +Y +AY  +++      +   +    + + PNLR DNT+ A  SP KG L F   +L +RR IF +  +  V  ++R FL+++    E  L  D  L   WH  F +D  V  V+P P+P RP  +     +TS  +     + + +F   + +C +  T  +   C
Sbjct:  236 LPYHMERLLELFRTCETIVSTLHNRSEVCSFDKIKPAVQEVVRCEFTEETVGKFAAVYPQAYKFRYDNQLDKLTKLVLSTYTLVLVPNLRTDNTQMARDSPSKGHLVFTGTRLIQRRHIFHKSLLARVLKAHRAFLISRLGVSESDLPGDSALRR-WHPSFALDTAVPEVEPAPLPPRPASSGRDARITSAREAVAAFRARALFR-EANVCEKLVTMPSTSTC 456
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Cavefish
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:ZFIN;Acc:ZDB-GENE-070111-1])

HSP 1 Score: 62.3882 bits (150), Expect = 1.239e-9
Identity = 71/337 (21.07%), Postives = 148/337 (43.92%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPV-NSPFSESHLGKIITIYHEAYLIKWER-LPSGNSTKPVKKIDITIKPNLRND-NTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFL--LNKCITPELINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSAN---------IDCKALPQSMIDKVNDNKMKRL----APDLHVKKGNN-----NVAMVSKLRDLVALLYTEFKAVQYKPLALSYLSN--IASVSLAVDENYFIDYLCYLIKLVPGWI 1997
            L +  K L E+F  +D +V  ++ + E    +++   +  + +  F E+H+G+I T+Y  AY  + E+ +P+ ++T       +T++P +  + N    ++S           +L +RR +F +  +E V   ++ FL  LN  +  E+ +D +    WH +F++D  V  + P  +P +P +T+            + +    +    + + L+ +  S           ++  A   S +  V+ + ++R+    A  L V    N      + M+S+L +L  +L   F A +   L +    N  +AS   ++       +L  L +L P W+
Sbjct:  310 LPYKYKLLAEMFRSMDTIVGMLFNRSETVTFAKVKQGVQDMMHKRFEEAHIGQIKTVYPSAYTFRQEKNIPNFSTTAKKSAYQLTVEPVIEEETNGVRPVLS---------ATRLLDRRRMFHQNLVEIVKGHHKVFLAGLNPLV--EVPDDKITR--WHPRFSVD-EVPNIQPGDLP-QPPQTEKLTTAQEVLDRARALMTPKMEKALANMALKTAEASCAKEPEPPAKPVETPAQTPSALKGVSQSLLERIRAKEAQKLQVAMTRNPEQEERLGMMSRLPELARILRNVFVAEKKPALIMEIACNRVVASYRSSLSSGEMEKHLRLLAELAPEWL 631
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Medaka
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:101175588])

HSP 1 Score: 69.707 bits (169), Expect = 5.752e-12
Identity = 73/331 (22.05%), Postives = 150/331 (45.32%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVN-SPFSESHLGKIITIYHEAYLIKWER-LPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCKFNIDLHVEL-VDPTPMPGRPEETDDFKEMTSFSQMYKIMK-KKNVFDCGSKICLRYSTNSANIDCKALP------------QSMIDKVNDNKMKRLAPDLHVKKGN-NNVAMVSKLRDLVALLYTEFKAVQYKPLALSYLSN--IASVSLAVDENYFIDYLCYLIKLVPGWI 1997
            L  H + L E+F  +D VV  +Y + E    +++   +  +    F ++H+ +I T++ EAY +K E+ +P+ +S     +  +T++P + +D++++      +  L      L ERR +F +  I  V   ++ FL +  + P  + +  L   WH +FN+DL   + V P P P   E     +E+   +++    K +K +     ++  R +  S      A P            QS++D++   + ++L   +     +   + M+S+L DL  +L   F A +   L ++   +  +AS + A+       ++  L +L   W+
Sbjct:  173 LPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQEKNIPTFSSGIKKGEYQLTVEPIIVSDHSQA------RPVLSASL--LLERRRVFHQNLISLVKQHHQEFL-SSLVPPVSVPEDRLTR-WHPRFNVDLVPAVPVSPLPEPPHRETLSTAQEVLDRARLLINPKMEKALISAAQQVQPRPAQGSVCAQKAAEPVAPVPGSLKGVSQSLLDRIRAKEAQKLQAVMTRDPAHEERLLMMSRLVDLARILRGIFVAEKKAALTMAAACSRMVASYASALTTGDMEKHVRLLAELAADWL 493

HSP 2 Score: 53.5286 bits (127), Expect = 5.817e-7
Identity = 41/165 (24.85%), Postives = 75/165 (45.45%), Query Frame = 3 
Query:  321 LPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNH-DFNERIFRQILNIFPESYFYEFN------STPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLE 794
            LPLHF++L ++F S+D  V  L +  ++    ++ + ++ M H  F +    QI  +FPE+Y  +        S+ IK  +Y          L+  P +     +    LS + +  R++     L+ +VKQ H  FL  +       E  L +   WH +F+++
Sbjct:  173 LPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQEKNIPTFSSGIKKGEY---------QLTVEPIIVSDHSQARPVLSASLLLERRRVFHQNLISLVKQHHQEFLSSLVPPVSVPEDRLTR---WHPRFNVD 325
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Medaka
Match: cdt1 (chromatin licensing and DNA replication factor 1 [Source:NCBI gene;Acc:101175588])

HSP 1 Score: 66.6254 bits (161), Expect = 5.855e-11
Identity = 73/318 (22.96%), Postives = 142/318 (44.65%), Query Frame = 3 
Query: 1062 LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVN-SPFSESHLGKIITIYHEAYLIKWER-LPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCKFNIDLHVEL-VDPTPMPGRPEETDDFKEMTSFSQMYKIMK-KKNVFDCGSKICLRYSTNSANIDCKALP------------QSMIDKVNDNKMKRLAPDLHVKKGN-NNVAMVSKLRDLVALL----YTEFKAVQYKPLALSYLSNIASVSLAVDENYF 1952
            L  H + L E+F  +D VV  +Y + E    +++   +  +    F ++H+ +I T++ EAY +K E+ +P+ +S     +  +T++P + +D++++      +  L      L ERR +F +  I  V   ++ FL +  + P  + +  L   WH +FN+DL   + V P P P   E     +E+   +++    K +K +     ++  R +  S      A P            QS++D++   + ++L   +     +   + M+S+L DL  +L      E KA      A S +    + +L   EN F
Sbjct:  223 LPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQEKNIPTFSSGIKKGEYQLTVEPIIVSDHSQA------RPVLSASL--LLERRRVFHQNLISLVKQHHQEFL-SSLVPPVSVPEDRLTR-WHPRFNVDLVPAVPVSPLPEPPHRETLSTAQEVLDRARLLINPKMEKALISAAQQVQPRPAQGSVCAQKAAEPVAPVPGSLKGVSQSLLDRIRAKEAQKLQAVMTRDPAHEERLLMMSRLVDLARILRGIFVAEKKAALTMAAACSRMVASYASALTTGENRF 530

HSP 2 Score: 53.5286 bits (127), Expect = 6.626e-7
Identity = 41/165 (24.85%), Postives = 75/165 (45.45%), Query Frame = 3 
Query:  321 LPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNH-DFNERIFRQILNIFPESYFYEFN------STPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLE 794
            LPLHF++L ++F S+D  V  L +  ++    ++ + ++ M H  F +    QI  +FPE+Y  +        S+ IK  +Y          L+  P +     +    LS + +  R++     L+ +VKQ H  FL  +       E  L +   WH +F+++
Sbjct:  223 LPLHFRLLAEMFRSMDTVVAMLYNRCETATFAKVQQGVQDMTHRRFEQNHVAQIRTVFPEAYALKQEKNIPTFSSGIKKGEY---------QLTVEPIIVSDHSQARPVLSASLLLERRRVFHQNLISLVKQHHQEFLSSLVPPVSVPEDRLTR---WHPRFNVD 375
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Planmine SMEST
Match: SMESG000017018.1 (SMESG000017018.1)

HSP 1 Score: 1239.94 bits (3207), Expect = 0.000e+0
Identity = 631/642 (98.29%), Postives = 636/642 (99.07%), Query Frame = 3 
Query:  207 MMSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLENCVEEIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNKLIGHXXXXXXXXXXXXXXXXXXXXXXXXEHSLKQRLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAMVSKLRDLVALLYTEFKAVQYKPLALSYLSNIASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQLSIDKSNLSIDDVLSAILLSGVSSLGNSDFSNIDTP 2132
            MMSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYIN KHEGNLIKVQEWHCKFDLENCVEEIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNKLIGHITNVIPNNNRNINVTSR SSSSNREHSLKQ+LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHC+FNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVA+VSKLRDLVALLYTEFKAVQYKPLA SYLSNIASVSLAVDENYFIDYLCYL+KLVPGWIEEL GPPPQLSIDKSN SIDDVLSAILLSG SSLGNSDFSNI+TP
Sbjct:    1 MMSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINDKHEGNLIKVQEWHCKFDLENCVEEIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNKLIGHITNVIPNNNRNINVTSRKSSSSNREHSLKQKLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCEFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAVVSKLRDLVALLYTEFKAVQYKPLAFSYLSNIASVSLAVDENYFIDYLCYLMKLVPGWIEELGGPPPQLSIDKSNFSIDDVLSAILLSGASSLGNSDFSNINTP 642
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Planmine SMEST
Match: SMESG000017018.1 (SMESG000017018.1)

HSP 1 Score: 1236.86 bits (3199), Expect = 0.000e+0
Identity = 630/641 (98.28%), Postives = 635/641 (99.06%), Query Frame = 3 
Query:  210 MSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLENCVEEIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNKLIGHXXXXXXXXXXXXXXXXXXXXXXXXEHSLKQRLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAMVSKLRDLVALLYTEFKAVQYKPLALSYLSNIASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQLSIDKSNLSIDDVLSAILLSGVSSLGNSDFSNIDTP 2132
            MSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYIN KHEGNLIKVQEWHCKFDLENCVEEIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNKLIGHITNVIPNNNRNINVTSR SSSSNREHSLKQ+LSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHC+FNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVA+VSKLRDLVALLYTEFKAVQYKPLA SYLSNIASVSLAVDENYFIDYLCYL+KLVPGWIEEL GPPPQLSIDKSN SIDDVLSAILLSG SSLGNSDFSNI+TP
Sbjct:    1 MSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYINDKHEGNLIKVQEWHCKFDLENCVEEIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNKLIGHITNVIPNNNRNINVTSRKSSSSNREHSLKQKLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCEFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAVVSKLRDLVALLYTEFKAVQYKPLAFSYLSNIASVSLAVDENYFIDYLCYLMKLVPGWIEELGGPPPQLSIDKSNFSIDDVLSAILLSGASSLGNSDFSNINTP 641
[Close]
BLAST of Chromatin licensing and DNA replication factor 1 vs. Planmine SMEST
Match: SMESG000017019.1 (SMESG000017019.1)

HSP 1 Score: 197.978 bits (502), Expect = 5.163e-53
Identity = 127/347 (36.60%), Postives = 191/347 (55.04%), Query Frame = 3 
Query: 1041 EHSLKQRLSFHLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKWERLPSGNSTKP-VKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLLNKCIT-PELINDYLLNESWHCKFNIDLHVELVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNSANIDCKALPQSMIDKVNDNKMKR---LAPDLHVKKGNNNVAMVSKLRDLVALLYTEFKAVQYKPLALSYLSNIASVSLAVDENYFIDYLCYLIKLVPGWIEELEGPPPQLSIDKSNLSIDDVL 2066
            E  LKQ+L  HLK L+E F +L+K+++D   K    +L   V  LS  +  F+E  LGKI+ +Y EAY ++ + +PS N  +    KIDI I+PNL + N           FL+FDF  +K+R   FLE C++ V  SYR F+LNK I   E + +++  E W+C FNI+L V+ V+P PMP + ++ +      +FS M K  KK   FD      L+   N       +L QS+IDKV  NK  R         +++      M SKL ++  ++  EFK    KPL  S L N+   SL ++E+ F +YL  L ++VPGW+++L    P+L+I   ++S+D+VL
Sbjct:  573 EFVLKQKLPAHLKVLYESFQELEKLIYDNRRKCATTYLRSAVEHLSN-HRQFTECQLGKILAVYPEAYSVRLQHVPSKNGLRSSTNKIDIAIRPNLESSN-----------FLKFDFATMKKRGQKFLENCVQIVVSSYREFILNKSIIHVENVEEHIRTEDWNCDFNIELDVKTVEPVPMPQKSQQKELENARLTFSVMKK-NKKPTEFDSSKVSPLKTGDNHR---LGSLSQSLIDKVRMNKANRSVITGAPATIRRN----FMASKLPEIATIIANEFKMFDNKPLERSQLVNLVCKSLQLNESSFSEYLDMLEQVVPGWVKDLNWAVPRLTIGTPDVSLDEVL 899

HSP 2 Score: 146.362 bits (368), Expect = 4.475e-36
Identity = 82/200 (41.00%), Postives = 124/200 (62.00%), Query Frame = 3 
Query:  252 KGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLFVSLDFAVNKLISSHQSLYINRITEVLKGMN-HDFNERIFRQILNIFPESYFYEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRKQEMQARLLKIVKQKHDLFLEKIGYIN---GKHEGNLIKVQE-WHCKFDLENCVEEIQPVFLDRN 836
            K KK  + V +   S   +N + +P HFK +LD+FVSLD  VNKL     S+YI+++ EVLK MN ++F+  IF QIL IFPESY YEF+S P+K+ ++++E++K++PN +FG   D  P+K +K   + Q+  R   +   LL  V+++H+ FL     IN    K E  +  + + WH  F+LE  V+ I+P+ LDRN
Sbjct:   33 KVKKTCATVSNNDSSYQSLNTILIPAHFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKIIPNFNFGNKSDFIPKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFL----LINKNLSKEEMEMFTLNKIWHEDFNLETSVQPIEPITLDRN 228

HSP 3 Score: 50.8322 bits (120), Expect = 3.329e-6
Identity = 45/168 (26.79%), Postives = 81/168 (48.21%), Query Frame = 3 
Query: 1071 HLKTLFELFIKLDKVVHDIYCKGELCFLSRIVSALSPVN-SPFSESHLGKIITIYHEAYLIKWERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKERRLIFLERCIEHVNFSYRRFLL-NKCITPELINDYLLNESWHCKFNIDLHVELVDP 1568
            H K + ++F+ LD VV+ +  +    ++ ++   L  +N + F     G+I+ I+ E+Y  +++  P   +    +K+ I   PN  N   KS  + P K    F   QL  R     E  +  V   +  FLL NK ++ E +  + LN+ WH  FN++  V+ ++P
Sbjct:   59 HFKNILDIFVSLDVVVNKLSKQRTSIYIDKVAEVLKEMNINNFDIGIFGQILRIFPESYSYEFDSPPLKVTRHYKEKMKII--PNF-NFGNKSDFI-PKKSQKVFRLDQLVSRMAHLYECLLGKVQERHNEFLLINKNLSKEEMEMFTLNKIWHEDFNLETSVQPIEP 222
The following BLAST results are available for this feature:
BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CDT11.319e-824.44chromatin licensing and DNA replication factor 1 [... [more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dup6.804e-826.92gene:FBgn0000996 transcript:FBtr0087387[more]
dup6.804e-826.92gene:FBgn0000996 transcript:FBtr0339999[more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CDT11.771e-625.57chromatin licensing and DNA replication factor 1 [... [more]
CDT12.045e-625.57chromatin licensing and DNA replication factor 1 [... [more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromatin licensing and DNA replication factor 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q9H211|CDT1_HUMAN6.335e-824.44DNA replication factor Cdt1 OS=Homo sapiens OX=960... [more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3PKA87.994e-2024.38DNA replication factor Cdt1 (Fragment) OS=Schistoc... [more]
A0A183TKL19.586e-2024.38DNA replication factor Cdt1 OS=Schistocephalus sol... [more]
A0A5J4NS124.821e-1825.00Chromatin licensing and DNA replication factor 1 O... [more]
A0A564ZB751.061e-1723.64CDT1 domain-containing protein OS=Hymenolepis dimi... [more]
A0A0R3U8Z61.497e-1728.25Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cdt11.239e-921.07chromatin licensing and DNA replication factor 1 [... [more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
cdt15.752e-1222.05chromatin licensing and DNA replication factor 1 [... [more]
cdt15.855e-1122.96chromatin licensing and DNA replication factor 1 [... [more]
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BLAST of Chromatin licensing and DNA replication factor 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000017018.10.000e+098.29SMESG000017018.1[more]
SMESG000017018.10.000e+098.28SMESG000017018.1[more]
SMESG000017019.15.163e-5336.60SMESG000017019.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014686 ID=SMED30014686|Name=Chromatin licensing and DNA replication factor 1|organism=Schmidtea mediterranea sexual|type=transcript|length=2152bp
GTTTTAATTTAATAAATTAAATATTTATTTTTGATATTTTTATAAATTTT
CAATAAGTAGAAATTTTACAATTATTTTATTTTATTTCATTTTATATTAA
ACGGAAAAGAAAATTTTAGAAAGAACAGATATGGAAAATTGCAAGTTAGC
CCGGCCCCCAATACTGTCACCCCTTTCGTGTTTTTTGCGATTATTTTAAA
TCATCAATGATGTCTTTGTTTGAAAATTCAGAACATTTAGTGGAGCACTA
TAAAGGAAAAAAGAAAATGTCTGCTGTTAAACATAAAGTTGATTCCAACT
CAATGATGAATAAAATGCCGCTGCCTCTTCATTTTAAAGTTCTTCTCGAT
CTATTCGTGTCATTGGATTTTGCTGTGAATAAACTGATATCCAGTCATCA
GTCGCTTTATATAAATCGAATTACGGAAGTTTTGAAAGGAATGAACCACG
ATTTTAATGAAAGAATATTTCGGCAAATTCTCAATATTTTCCCCGAATCA
TATTTCTATGAATTCAATTCAACTCCAATTAAACTTGCGAAATATTTCCG
GGAACAACTGAAATTGGTTCCAAATTTGAGTTTTGGTCCAGCAGTCGATT
GTTCCCCAAGAAAATTAGAAAAGTGCCTAAGTGTTGCTCAAATAACTTTC
CGAAAGCAAGAAATGCAGGCGCGATTATTGAAAATTGTCAAACAAAAACA
CGACTTATTCTTAGAAAAAATCGGCTATATCAACGGTAAACACGAGGGGA
ACTTGATAAAGGTCCAAGAATGGCATTGTAAATTCGATTTGGAAAATTGT
GTAGAGGAAATCCAGCCGGTTTTTCTTGATAGGAATTTCAAATATCCAAT
TAAGCAAACCATTATTAAAAAACCCGAATCAGAAAAGGTGTTCAGAAATA
TTTTTAAAGTGCCAACTAATTCCGGAAACCAAATTTCGAAAGACCAATTA
AATAAGTTAATTGGTCACATCACGAATGTTATACCGAACAACAACAGGAA
TATCAATGTCACATCAAGAAATTCCAGTTCCAGCAATCGGGAACATTCTC
TTAAACAAAGGCTTTCTTTTCATCTTAAAACTCTTTTTGAGTTGTTCATC
AAGCTGGATAAAGTCGTGCATGATATCTACTGTAAAGGTGAACTGTGCTT
TCTATCAAGAATCGTTTCTGCACTGTCGCCTGTCAATAGCCCGTTTTCTG
AGTCGCATCTCGGGAAAATTATCACAATCTATCATGAAGCGTATTTAATT
AAATGGGAGCGACTGCCGTCGGGTAACTCTACTAAACCTGTCAAGAAAAT
TGATATTACTATTAAGCCGAATCTGAGGAACGATAATACTAAATCAGCTC
TTATGTCTCCGGATAAAGGGTTTTTGAGGTTCGATTTTGTCCAATTGAAA
GAGAGGCGCCTTATATTTCTCGAAAGGTGCATTGAGCACGTCAATTTTAG
CTACCGTCGATTTCTATTGAACAAATGTATTACCCCAGAACTAATCAATG
ACTATCTTCTGAATGAATCTTGGCATTGTAAATTCAATATTGACCTTCAT
GTTGAATTGGTTGATCCTACACCAATGCCAGGCAGACCTGAGGAAACCGA
TGATTTTAAAGAGATGACAAGTTTTTCTCAAATGTACAAAATTATGAAAA
AGAAAAATGTATTTGATTGCGGATCAAAAATTTGTCTCAGATATTCAACT
AATTCCGCAAATATTGACTGTAAAGCTTTGCCCCAATCAATGATAGATAA
AGTTAATGATAACAAAATGAAAAGGTTGGCACCCGATCTACATGTGAAGA
AGGGAAATAATAACGTTGCCATGGTATCCAAATTAAGGGATCTAGTGGCA
CTCCTGTATACGGAATTCAAAGCCGTCCAGTATAAGCCGTTAGCTTTGTC
GTACCTTTCAAATATCGCTAGCGTTTCATTGGCTGTGGATGAGAATTATT
TTATTGATTATTTGTGTTATTTGATTAAATTGGTTCCCGGGTGGATTGAG
GAGCTCGAGGGCCCCCCTCCTCAGTTGTCGATTGACAAATCAAATCTCAG
CATAGATGATGTATTATCAGCAATATTACTCTCTGGTGTCTCCTCATTGG
GCAATTCCGATTTCTCCAATATTGATACGCCGTGATCTTGATTATTTGTG
TG
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protein sequence of SMED30014686-orf-1

>SMED30014686-orf-1 ID=SMED30014686-orf-1|Name=SMED30014686-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=643bp
MMSLFENSEHLVEHYKGKKKMSAVKHKVDSNSMMNKMPLPLHFKVLLDLF
VSLDFAVNKLISSHQSLYINRITEVLKGMNHDFNERIFRQILNIFPESYF
YEFNSTPIKLAKYFREQLKLVPNLSFGPAVDCSPRKLEKCLSVAQITFRK
QEMQARLLKIVKQKHDLFLEKIGYINGKHEGNLIKVQEWHCKFDLENCVE
EIQPVFLDRNFKYPIKQTIIKKPESEKVFRNIFKVPTNSGNQISKDQLNK
LIGHITNVIPNNNRNINVTSRNSSSSNREHSLKQRLSFHLKTLFELFIKL
DKVVHDIYCKGELCFLSRIVSALSPVNSPFSESHLGKIITIYHEAYLIKW
ERLPSGNSTKPVKKIDITIKPNLRNDNTKSALMSPDKGFLRFDFVQLKER
RLIFLERCIEHVNFSYRRFLLNKCITPELINDYLLNESWHCKFNIDLHVE
LVDPTPMPGRPEETDDFKEMTSFSQMYKIMKKKNVFDCGSKICLRYSTNS
ANIDCKALPQSMIDKVNDNKMKRLAPDLHVKKGNNNVAMVSKLRDLVALL
YTEFKAVQYKPLALSYLSNIASVSLAVDENYFIDYLCYLIKLVPGWIEEL
EGPPPQLSIDKSNLSIDDVLSAILLSGVSSLGNSDFSNIDTP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000278mitotic cell cycle
GO:0000076DNA replication checkpoint
GO:0000082G1/S transition of mitotic cell cycle
GO:0000083regulation of transcription involved in G1/S transition of mitotic cell cycle
GO:0006260DNA replication
GO:0007049cell cycle
GO:0007059chromosome segregation
GO:0030174regulation of DNA-dependent DNA replication initiation
GO:0033262regulation of nuclear cell cycle DNA replication
GO:0051315attachment of mitotic spindle microtubules to kinetochore
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036390WH_DNA-bd_sf
IPR014939CDT1_Gemini-bd-like
IPR011991WHTH_DNA-bd_dom
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005829cytosol
GO:0016604nuclear body
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014939CDT1 Geminin-binding domain-likeSMARTSM01075CDT1_2coord: 39..209
e-value: 3.8E-4
score: 16.6
coord: 286..459
e-value: 2.0E-4
score: 20.3
IPR014939CDT1 Geminin-binding domain-likePFAMPF08839CDT1coord: 288..458
e-value: 2.6E-20
score: 73.4
coord: 39..204
e-value: 2.2E-19
score: 70.4
NoneNo IPR availablePANTHERPTHR28637FAMILY NOT NAMEDcoord: 266..496
coord: 17..198
NoneNo IPR availablePANTHERPTHR28637:SF1DNA REPLICATION FACTOR CDT1coord: 266..496
coord: 17..198
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 274..461
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 30..200