Reticulocalbin-2

Overview
NameReticulocalbin-2
Smed IDSMED30014680
Length (bp)1043
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Reticulocalbin-2 (SMED30014680) t-SNE clustered cells

Violin plots show distribution of expression levels for Reticulocalbin-2 (SMED30014680) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Reticulocalbin-2 (SMED30014680) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Reticulocalbin-2 (SMED30014680) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30014680

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30014680h1SMcG0018219 SmedASXL_008487SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30014680h1SMcG0018219 SmedASXL_008487SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30014680h1SMcG0018219 dd_Smed_v4_7654_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Reticulocalbin-2 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 96.2857 bits (238), Expect = 5.028e-22
Identity = 74/245 (30.20%), Postives = 124/245 (50.61%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T DEL +WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 96.2857 bits (238), Expect = 5.645e-22
Identity = 74/245 (30.20%), Postives = 124/245 (50.61%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T DEL +WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   77 SKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 314          
BLAST of Reticulocalbin-2 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 90.8929 bits (224), Expect = 4.018e-20
Identity = 74/245 (30.20%), Postives = 121/245 (49.39%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  ++ KID+DK+  +T+ EL  WI     K +    ++    +D+N+D  I+W E    TYG         L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYG-------TYLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Human
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 90.8929 bits (224), Expect = 4.047e-20
Identity = 74/245 (30.20%), Postives = 121/245 (49.39%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  ++ KID+DK+  +T+ EL  WI     K +    ++    +D+N+D  I+W E    TYG         L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   77 SKERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYG-------TYLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 314          
BLAST of Reticulocalbin-2 vs. Ensembl Human
Match: RCN3 (reticulocalbin 3 [Source:HGNC Symbol;Acc:HGNC:21145])

HSP 1 Score: 86.6557 bits (213), Expect = 1.703e-18
Identity = 72/257 (28.02%), Postives = 114/257 (44.36%), Query Frame = 1
Query:  226 SVTKLRELLVKID--SDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L  ++ ++D   D +  ++  EL  WI     + +     +    YD ++D ++ W E+   TYG+     E      D  D + Y+  +  D  RF+ AD D D   T+EE  + L+P   P +  + + + L   D++ D  +  +E+   LY     E     +      F  + D NKD  L   E+  W  P        EAN L+ +SDT+ D  L+  EIL   N +  SQAT+YG   T   DEL
Sbjct:   76 SQARLGRIVDRMDRAGDGDGWVSLAELRAWIAHTQQRHIRDSVSAAWDTYDTDRDGRVGWEELRNATYGHYAPGEE----FHDVEDAETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVIAETLEDLDRNKDGYVQVEEYIADLYSAEPGEEEPAWVQTERQQFRDFRDLNKDGHLDGSEVGHWVLPPAQDQPLVEANHLLHESDTDKDGRLSKAEILGNWNMFVGSQATNYGEDLTRHHDEL 328          
BLAST of Reticulocalbin-2 vs. Ensembl Celegans
Match: calu-2 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:Q9TYS8])

HSP 1 Score: 88.5817 bits (218), Expect = 7.493e-20
Identity = 78/257 (30.35%), Postives = 120/257 (46.69%), Query Frame = 1
Query:  190 SEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            SEQ +    G  SV K  + L   D++ +  + K E++ W+     K +D+ A   IS  D N D  ++W E L  ++       E++ N      K+    I  D+  FK AD D DG L  EE  S LNP   P +  V +   L   D++ D  + E+EF L     +  +E   +E E     YD NKD KL+ DEL  W   D   + ++EA SL+  SD + D  L+++EI+     +  ++A     H+ 
Sbjct:   37 SEQKLNLKSGQESVEKFAKAL---DTNNDGFVDKSEILAWVSESYQKTVDREAVERISELDENADGFVSWEEYLADSF-----PDEELHN------KEEESLIAQDKMYFKQADEDNDGKLNLEELASFLNPEHHPHMHPVLIAVTLLEKDQNGDGAIEEKEF-LGELDEQRGSEWYKVEVERFRTVYDKNKDGKLAGDELTDWLLVDGTTAGSYEAESLLTNSDDDKDGQLSYEEIVKHHALFAKTEAAQEADHLH 278          
BLAST of Reticulocalbin-2 vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 58.9214 bits (141), Expect = 1.321e-9
Identity = 64/258 (24.81%), Postives = 115/258 (44.57%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLK----DLDKRAKSDISFYDINK--DNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXX------XXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            S  KL +L+ K+D+D +  I ++EL + I  +  +    D+D+  K+    Y   K  D KI W +     YG  + + +++         +  + I  D  R+  AD D +G L + EY   ++P     +  V + + +   DK+ D  ++  E+    Y  +            +     +F ++ D + D KL+ +E+  W  P     A  EA  L+  +D N D  L  DEI++  + +  SQAT YG  ++
Sbjct:   61 SKEKLAKLVPKMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKN----YKAEKIVDGKIKWEDYREMVYGSADGAGQEL-------SPEYAKMIARDEKRWAVADYDSNGALDRTEYGCFMHPEDCDHMRDVVVAETVDDIDKNKDGSVDLDEYIGDMYRPDDYPELNGKEPDWVQSEREMFKEHRDKDGDGKLNQEEMRDWIMPVGFDHAEAEARHLVGIADDNKDGKLNLDEIVAHYDTFVGSQATDYGEQLQ 307          
BLAST of Reticulocalbin-2 vs. Ensembl Celegans
Match: calu-1 (CALUmenin (Calcium-binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:H2L0I1])

HSP 1 Score: 58.9214 bits (141), Expect = 1.321e-9
Identity = 64/258 (24.81%), Postives = 115/258 (44.57%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLK----DLDKRAKSDISFYDINK--DNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXX------XXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            S  KL +L+ K+D+D +  I ++EL + I  +  +    D+D+  K+    Y   K  D KI W +     YG  + + +++         +  + I  D  R+  AD D +G L + EY   ++P     +  V + + +   DK+ D  ++  E+    Y  +            +     +F ++ D + D KL+ +E+  W  P     A  EA  L+  +D N D  L  DEI++  + +  SQAT YG  ++
Sbjct:   61 SKEKLAKLVPKMDADSDGFIEENELKDHINFMQKRYVNNDVDRTWKN----YKAEKIVDGKIKWEDYREMVYGSADGAGQEL-------SPEYAKMIARDEKRWAVADYDSNGALDRTEYGCFMHPEDCDHMRDVVVAETVDDIDKNKDGSVDLDEYIGDMYRPDDYPELNGKEPDWVQSEREMFKEHRDKDGDGKLNQEEMRDWIMPVGFDHAEAEARHLVGIADDNKDGKLNLDEIVAHYDTFVGSQATDYGEQLQ 307          
BLAST of Reticulocalbin-2 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301907)

HSP 1 Score: 97.8265 bits (242), Expect = 8.099e-23
Identity = 85/271 (31.37%), Postives = 139/271 (51.29%), Query Frame = 1
Query:  169 EEILPNFSEQSITDNFGSYSVTKLRELLVKI-DSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD--NKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ----FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            E I+ N  E    D+       +   +L+K+ D +K+E I + EL  WI+  S K L +   +D  F +I++D   +ITW E L+ TY   +E  +K     + ID D+Y+     I  D++ F AAD+++DG LT EE+    NP   P++L + ++  +   D D+D ++N QEF +    +    E  + E E     +D N D  L+ DE+  W  P     AN E + L   +D + D  L++ EIL+  + +  S+AT YG H++
Sbjct:   71 EAIIGNTKEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILR-SFKKLSEEEAAD-RFEEIDQDADERITWKEYLQDTYAMEDEDFKK-----ETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEF-VGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQ 333          
BLAST of Reticulocalbin-2 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301905)

HSP 1 Score: 97.8265 bits (242), Expect = 8.099e-23
Identity = 85/271 (31.37%), Postives = 139/271 (51.29%), Query Frame = 1
Query:  169 EEILPNFSEQSITDNFGSYSVTKLRELLVKI-DSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD--NKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ----FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            E I+ N  E    D+       +   +L+K+ D +K+E I + EL  WI+  S K L +   +D  F +I++D   +ITW E L+ TY   +E  +K     + ID D+Y+     I  D++ F AAD+++DG LT EE+    NP   P++L + ++  +   D D+D ++N QEF +    +    E  + E E     +D N D  L+ DE+  W  P     AN E + L   +D + D  L++ EIL+  + +  S+AT YG H++
Sbjct:   71 EAIIGNTKEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILR-SFKKLSEEEAAD-RFEEIDQDADERITWKEYLQDTYAMEDEDFKK-----ETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEF-VGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQ 333          
BLAST of Reticulocalbin-2 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079033)

HSP 1 Score: 97.8265 bits (242), Expect = 8.099e-23
Identity = 85/271 (31.37%), Postives = 139/271 (51.29%), Query Frame = 1
Query:  169 EEILPNFSEQSITDNFGSYSVTKLRELLVKI-DSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD--NKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ----FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            E I+ N  E    D+       +   +L+K+ D +K+E I + EL  WI+  S K L +   +D  F +I++D   +ITW E L+ TY   +E  +K     + ID D+Y+     I  D++ F AAD+++DG LT EE+    NP   P++L + ++  +   D D+D ++N QEF +    +    E  + E E     +D N D  L+ DE+  W  P     AN E + L   +D + D  L++ EIL+  + +  S+AT YG H++
Sbjct:   71 EAIIGNTKEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILR-SFKKLSEEEAAD-RFEEIDQDADERITWKEYLQDTYAMEDEDFKK-----ETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEF-VGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQ 333          
BLAST of Reticulocalbin-2 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0079034)

HSP 1 Score: 97.8265 bits (242), Expect = 8.099e-23
Identity = 85/271 (31.37%), Postives = 139/271 (51.29%), Query Frame = 1
Query:  169 EEILPNFSEQSITDNFGSYSVTKLRELLVKI-DSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD--NKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ----FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            E I+ N  E    D+       +   +L+K+ D +K+E I + EL  WI+  S K L +   +D  F +I++D   +ITW E L+ TY   +E  +K     + ID D+Y+     I  D++ F AAD+++DG LT EE+    NP   P++L + ++  +   D D+D ++N QEF +    +    E  + E E     +D N D  L+ DE+  W  P     AN E + L   +D + D  L++ EIL+  + +  S+AT YG H++
Sbjct:   71 EAIIGNTKEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILR-SFKKLSEEEAAD-RFEEIDQDADERITWKEYLQDTYAMEDEDFKK-----ETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEF-VGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQ 333          
BLAST of Reticulocalbin-2 vs. Ensembl Fly
Match: CG31650 (gene:FBgn0031673 transcript:FBtr0301906)

HSP 1 Score: 97.8265 bits (242), Expect = 8.099e-23
Identity = 85/271 (31.37%), Postives = 139/271 (51.29%), Query Frame = 1
Query:  169 EEILPNFSEQSITDNFGSYSVTKLRELLVKI-DSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD--NKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ----FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            E I+ N  E    D+       +   +L+K+ D +K+E I + EL  WI+  S K L +   +D  F +I++D   +ITW E L+ TY   +E  +K     + ID D+Y+     I  D++ F AAD+++DG LT EE+    NP   P++L + ++  +   D D+D ++N QEF +    +    E  + E E     +D N D  L+ DE+  W  P     AN E + L   +D + D  L++ EIL+  + +  S+AT YG H++
Sbjct:   71 EAIIGNTKEAQEFDSLSPDESKRRLLILIKMMDLNKDEFIDRHELKAWILR-SFKKLSEEEAAD-RFEEIDQDADERITWKEYLQDTYAMEDEDFKK-----ETIDYDSYEDEQKMIKQDKEMFNAADTNKDGVLTLEEFVLFQNPEEHPQMLPILLEHTMQDKDADHDGKINFQEF-VGDAASHHDKEWLITEKERFDKDHDSNGDGVLTGDEVLSWIVPSNTAIANDEVDHLFVSTDEDHDDRLSYLEILNNYDTFVGSEATDYGDHLQ 333          
BLAST of Reticulocalbin-2 vs. Ensembl Zebrafish
Match: rcn1 (reticulocalbin 1, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-080225-33])

HSP 1 Score: 99.3673 bits (246), Expect = 2.773e-23
Identity = 78/255 (30.59%), Postives = 134/255 (52.55%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L +++ +IDS+ +  IT DEL  WI  +  + + +      + YD+NKDNKI+W E  + TYGY   + E+    +D  D+ +++  +  D  RFK AD D +    +EE+ + L+P     + ++ + + L   DK+ D  ++E E+   ++ +         +    + F  + D NKD K+ ++E+  W  P     A  EA  L+ +SDT+ D++L+ +EIL   N +  SQAT+YG   T   DEL
Sbjct:   69 SKARLGKIVERIDSNVDGFITTDELKAWIKRVQKRYVYENVAKVWTDYDLNKDNKISWDEYKQATYGYYLANPEEF---EDATDQFSFKKMLPRDERRFKTADLDGNLAADKEEFTAFLHPEEFAHMQEIVVLETLEDIDKNGDGHVDEDEYIADMFAHEDAGPEPDWVRTERDQFSDFRDLNKDGKMDLEEIRHWILPQDYDHAQAEARHLVYESDTDKDQMLSKEEILENWNMFVGSQATNYGEDLTRNHDEL 320          
BLAST of Reticulocalbin-2 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 97.0561 bits (240), Expect = 1.578e-22
Identity = 80/254 (31.50%), Postives = 131/254 (51.57%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVD-DRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGT--HIRDEL 969
            S  +L +++ KID+DK+  ++  EL  WI     + +++      + YD NKD KI WIE   TTYGY    ++   +  D  DK  Y+ +++ D  RFK+AD D DG  T+EE+ + L+P     +  + + + +   DK+ D +++ QE+   +Y     E     +   +  F ++ D NKD  L   E+  W  P     A+ EA  L+ ++D + D  L+ +EI+ KL+  K S  T YGT   + DEL
Sbjct:   67 SKDRLGKIVDKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGY---YIDTEFDDVD--DKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEEFDFMRDIVIQETIEDIDKNGDGKIDLQEYIGDMYNPEDGETEPDWVTTEKKQFSEFRDMNKDGFLDATEVSHWILPTEVDHADNEARHLIHETDKDNDGRLSINEIMDKLDFIKVSTITDYGTLIEVHDEL 315          
BLAST of Reticulocalbin-2 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 97.0561 bits (240), Expect = 1.578e-22
Identity = 80/254 (31.50%), Postives = 131/254 (51.57%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVD-DRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGT--HIRDEL 969
            S  +L +++ KID+DK+  ++  EL  WI     + +++      + YD NKD KI WIE   TTYGY    ++   +  D  DK  Y+ +++ D  RFK+AD D DG  T+EE+ + L+P     +  + + + +   DK+ D +++ QE+   +Y     E     +   +  F ++ D NKD  L   E+  W  P     A+ EA  L+ ++D + D  L+ +EI+ KL+  K S  T YGT   + DEL
Sbjct:   67 SKDRLGKIVDKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGY---YIDTEFDDVD--DKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEEFDFMRDIVIQETIEDIDKNGDGKIDLQEYIGDMYNPEDGETEPDWVTTEKKQFSEFRDMNKDGFLDATEVSHWILPTEVDHADNEARHLIHETDKDNDGRLSINEIMDKLDFIKVSTITDYGTLIEVHDEL 315          
BLAST of Reticulocalbin-2 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 96.6709 bits (239), Expect = 2.055e-22
Identity = 81/255 (31.76%), Postives = 130/255 (50.98%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVD-DRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L +++ KID+DK+  ++  EL  WI     + +++      + YD NKD KI WIE   TTYGY    ++   +  D  DK  Y+ +++ D  RFK+AD D DG  T+EE+ + L+P     +  + + + +   DK+ D +++ QE+   +Y     E     +   +  F ++ D NKD  L   E+  W  P     A+ EA  L+ ++D + D  +T  EIL   N +  SQAT+YG   T   DEL
Sbjct:   67 SKDRLGKIVDKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGY---YIDTEFDDVD--DKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEEFDFMRDIVIQETIEDIDKNGDGKIDLQEYIGDMYNPEDGETEPDWVTTEKKQFSEFRDMNKDGFLDATEVSHWILPTEVDHADNEARHLIHETDKDNDDKITKKEILENWNMFVGSQATNYGEDLTKRHDEL 316          
BLAST of Reticulocalbin-2 vs. Ensembl Zebrafish
Match: rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:ZFIN;Acc:ZDB-GENE-040625-175])

HSP 1 Score: 96.6709 bits (239), Expect = 2.055e-22
Identity = 81/255 (31.76%), Postives = 130/255 (50.98%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVD-DRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L +++ KID+DK+  ++  EL  WI     + +++      + YD NKD KI WIE   TTYGY    ++   +  D  DK  Y+ +++ D  RFK+AD D DG  T+EE+ + L+P     +  + + + +   DK+ D +++ QE+   +Y     E     +   +  F ++ D NKD  L   E+  W  P     A+ EA  L+ ++D + D  +T  EIL   N +  SQAT+YG   T   DEL
Sbjct:   67 SKDRLGKIVDKIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWNEYDQNKDGKIGWIEYKNTTYGY---YIDTEFDDVD--DKATYKSMLNRDERRFKSADRDGDGVATREEFTAFLHPEEFDFMRDIVIQETIEDIDKNGDGKIDLQEYIGDMYNPEDGETEPDWVTTEKKQFSEFRDMNKDGFLDATEVSHWILPTEVDHADNEARHLIHETDKDNDDKITKKEILENWNMFVGSQATNYGEDLTKRHDEL 316          
BLAST of Reticulocalbin-2 vs. Ensembl Xenopus
Match: fgf11 (fibroblast growth factor 11 [Source:Xenbase;Acc:XB-GENE-479522])

HSP 1 Score: 92.8189 bits (229), Expect = 8.012e-21
Identity = 76/254 (29.92%), Postives = 127/254 (50.00%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L +++ +IDSD + +IT +EL  WI  +  + + +        YD+NKDN I+W E  + TYGY   + E+  ++ +       + +  D  RFK AD + D    +EE+ S L+P     +  + + + L   DK+ D  +++ E+   ++ +   +     +      F  + D NKD K+  +E+  W  P     A  EA  L+ +SD + DKVLT +E+L   N +  SQAT+YG   T   DEL
Sbjct:   70 SKDRLGKIINRIDSDSDGLITTEELTAWIKRVQKRYVYENVARVWKDYDVNKDNTISWEEYKQATYGYYLANPEEFQDAAEQFS--FKKMLPRDERRFKQADLNGDLEANREEFTSFLHPEEFEHMKDIVILETLEDIDKNGDGFVDQDEYIADMFSHEEGQPEPDWVATEREQFADFRDINKDGKMDKEEIRHWILPQDYDHAQAEARHLIYESDLDKDKVLTKEEVLQNWNMFVGSQATNYGEDLTRNHDEL 321          
BLAST of Reticulocalbin-2 vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 92.0485 bits (227), Expect = 1.321e-20
Identity = 73/245 (29.80%), Postives = 125/245 (51.02%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  ++ KID+DK+  +T +EL +WII    + + +  +     +D+N D+ ++W E    TYGY       IL+  DP +  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +  +      +      F+++ D N D K+  +E + W  P     A  E+  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGRIVGKIDADKDGYVTVNELRDWIIFAQKRWIYEDVERQWKGHDLNGDSMVSWEEYKNATYGY-------ILDDPDPDNSFNYKQMMVRDERRFKMADQDGDLIATKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPEWVKTEREQFVEFRDKNHDGKMDKEETKDWILPSDYDHAEAESRHLVYESDQNKDSKLTREEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 90.1225 bits (222), Expect = 8.693e-20
Identity = 77/249 (30.92%), Postives = 125/249 (50.20%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLS----LKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ ++D D ++ IT  EL  WI  +S    L+D +K+ K     YD NKD  I+W E  +  YG+     E+  +  D  DK+ Y+  ++ D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK++D  ++  E+   +Y    +E     +      F  + D NKD K+   E+ +W  P     A+ EA  L+ +SD + D  LT  EIL   N +  SQAT+YG
Sbjct:   70 SQHRLGKIIDQMDKDNDKYITSGELFAWIKHVSRRWNLEDSEKQGKK----YDTNKDGMISWDEYAKGVYGHLLGKGEEFYDVADK-DKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEEYGYMQDIVITETIEDIDKNDDGIVDVHEYIADMYTPNEDEPEPDWVKTERQQFTDFRDINKDGKMDRTEISQWILPHDYDHADLEAKHLVYESDKDKDGKLTKKEILDNWNMFVGSQATNYG 313          
BLAST of Reticulocalbin-2 vs. Ensembl Xenopus
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:395023])

HSP 1 Score: 88.5817 bits (218), Expect = 2.768e-19
Identity = 77/245 (31.43%), Postives = 123/245 (50.20%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T  EL EW+     + + +      + YD NKD+ I+W E   TTYGY     E+  +  D  DK+ Y+  ++ D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK++D  ++  E+   +Y    +E     +      F  + D NKD K+   E+ +W  P     A+ EA  L+ +SD + D  LT  EIL   N +  SQAT+YG
Sbjct:   70 SQHRLGKIVDKIDKDKDGFVTDIELKEWMKHSQNRYIYENVNKHWADYDKNKDDMISWEEYKNTTYGYL--PGEEFYDVADK-DKERYRKMMMRDERRFKVADKDGDLIATREEFTAFLHPEEYGYMQDIVITETIEDIDKNDDGIVDVHEYIADMYTPNEDEPEPDWVKTERQQFTDFRDINKDGKMDRTEISQWILPHDYDHADLEAKHLVYESDKDKDGKLTKKEILDNWNMFVGSQATNYG 311          
BLAST of Reticulocalbin-2 vs. Ensembl Xenopus
Match: calu (calumenin [Source:NCBI gene;Acc:407965])

HSP 1 Score: 82.0333 bits (201), Expect = 4.257e-17
Identity = 70/245 (28.57%), Postives = 118/245 (48.16%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  ++ KID D +  +T+ EL  WI     K +    +     +D+N+D  ++W E    TYG         L+  DP +  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +  +      +      F+++ D N D K+  +E + W  P     A  E+  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGMIVSKIDLDNDGYVTEGELTAWIKKAQKKYVYDNVERQWQEFDLNQDGLVSWDEYRNVTYG-------TYLDDPDPDNSFNYKQMMVRDERRFKMADQDGDLIATKEEFTAFLHPEEFDYMKDIVVLETMEDIDKNGDGLIDLEEYIGDMYNHDGDANEPEWVKTEREQFVEFRDKNHDGKMDKEETKDWILPSDYDHAEAESRHLVYESDQNKDSKLTREEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 95.9005 bits (237), Expect = 4.169e-22
Identity = 74/245 (30.20%), Postives = 123/245 (50.20%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T DEL  WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGKIVSKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Mouse
Match: Calu (calumenin [Source:MGI Symbol;Acc:MGI:1097158])

HSP 1 Score: 90.8929 bits (224), Expect = 2.500e-20
Identity = 74/245 (30.20%), Postives = 121/245 (49.39%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  ++ KID+DK+  +T+ EL  WI     K +    ++    +D+N+D  I+W E    TYG         L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGMIVDKIDADKDGFVTEGELKSWIKHAQKKYIYDNVENQWQEFDMNQDGLISWDEYRNVTYG-------TYLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Mouse
Match: Rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:MGI Symbol;Acc:MGI:1277122])

HSP 1 Score: 90.8929 bits (224), Expect = 2.903e-20
Identity = 75/257 (29.18%), Postives = 118/257 (45.91%), Query Frame = 1
Query:  226 SVTKLRELLVKID--SDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L  ++ ++D   D +  ++  EL  WI     + +     +    YD ++D ++ W E+   TYG+ E   E      D  D + Y+  +  D  RF+ AD D D   T+EE  + L+P   P +  + + + L   DK+ D  +  +E+   LY     E     +      F ++ D NKD +L   E+  W  P        EAN L+ +SDT+ D  L+  EILS  N +  SQAT+YG   T   DEL
Sbjct:   76 SQARLGRIVDRMDLAGDSDGWVSLAELRAWIAHTQQRHIRDSVSAAWHTYDTDRDGRVGWEELRNATYGHYEPGEE----FHDVEDAETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIADLYSEEPGEEEPAWVQTERQQFREFRDLNKDGRLDGSEVGYWVLPPSQDQPLVEANHLLHESDTDKDGRLSKAEILSNWNMFVGSQATNYGEDLTRHHDEL 328          
BLAST of Reticulocalbin-2 vs. Ensembl Mouse
Match: Rcn3 (reticulocalbin 3, EF-hand calcium binding domain [Source:MGI Symbol;Acc:MGI:1277122])

HSP 1 Score: 90.8929 bits (224), Expect = 2.903e-20
Identity = 75/257 (29.18%), Postives = 118/257 (45.91%), Query Frame = 1
Query:  226 SVTKLRELLVKID--SDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L  ++ ++D   D +  ++  EL  WI     + +     +    YD ++D ++ W E+   TYG+ E   E      D  D + Y+  +  D  RF+ AD D D   T+EE  + L+P   P +  + + + L   DK+ D  +  +E+   LY     E     +      F ++ D NKD +L   E+  W  P        EAN L+ +SDT+ D  L+  EILS  N +  SQAT+YG   T   DEL
Sbjct:   76 SQARLGRIVDRMDLAGDSDGWVSLAELRAWIAHTQQRHIRDSVSAAWHTYDTDRDGRVGWEELRNATYGHYEPGEE----FHDVEDAETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHMRDIVVAETLEDLDKNKDGYVQVEEYIADLYSEEPGEEEPAWVQTERQQFREFRDLNKDGRLDGSEVGYWVLPPSQDQPLVEANHLLHESDTDKDGRLSKAEILSNWNMFVGSQATNYGEDLTRHHDEL 328          
BLAST of Reticulocalbin-2 vs. Ensembl Mouse
Match: Rcn1 (reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559])

HSP 1 Score: 88.5817 bits (218), Expect = 2.357e-19
Identity = 76/247 (30.77%), Postives = 125/247 (50.61%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQF---IVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ +IDSD + ++T +EL  WI  +  + +          YD +KD KI+W E  + TYGY   +  +  +S D     ++ F   +  D  RFKA+D D D   T+EE+ + L+P     + ++ + + L   DK+ D  +++ E+   ++ +         +L     F  + D NKD KL  DE+  W  P     A  EA  L+ +SD N D++LT +EIL   N +  SQAT+YG
Sbjct:   74 SKERLGKIVDRIDSDGDGLVTTEELKLWIKRVQKRYIYDNVAKVWKDYDRDKDEKISWEEYKQATYGYYLGNPAEFHDSSD-----HHTFKKMLPRDERRFKASDLDGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADMFSHEDNGPEPDWVLSEREQFNDFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEMLTKEEILDNWNMFVGSQATNYG 315          
BLAST of Reticulocalbin-2 vs. UniProt/SwissProt
Match: sp|O93434|RCN1_TAKRU (Reticulocalbin-1 OS=Takifugu rubripes OX=31033 GN=rcn1 PE=3 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.020e-24
Identity = 83/259 (32.05%), Postives = 129/259 (49.81%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQF-----IVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L +++ +ID D N  IT DEL  WI  +  + + +      + YD+NKDNKI+W E  + TYGY        L++ +  D+   QF     +  D  RFK AD D D    +EE+ S L+P     +  + + + L   DK++D  ++E E+   ++ +         +      F  + D NKD K+ +DE+  W  P     A  EA  L+ +SD + D++LT +EIL   N +  SQAT+YG   T   DEL
Sbjct:   71 SKDRLSKIVDRIDGDGNSYITTDELKAWIKRVQKRYVYENVVKVWADYDLNKDNKISWEEYKQATYGY-------YLSNPEEFDETTDQFSFKKMLPRDERRFKRADLDGDSAANREEFTSFLHPEEFEHMKDIVVLETLEDIDKNSDGHVDEDEYIADMFAHEDRGPEPEWVKTEREQFSDFRDLNKDGKMDLDEIRHWIMPQDYDHAQAEARHLVYESDKDKDQMLTKEEILDNWNMFVGSQATNYGEDLTRNHDEL 322          
BLAST of Reticulocalbin-2 vs. UniProt/SwissProt
Match: sp|Q6XLQ7|CALU_RABIT (Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU PE=1 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 1.303e-21
Identity = 74/245 (30.20%), Postives = 125/245 (51.02%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID+DK+  +T DEL +WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGKIVSKIDADKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNGDGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. UniProt/SwissProt
Match: sp|O43852|CALU_HUMAN (Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2)

HSP 1 Score: 96.2857 bits (238), Expect = 2.415e-21
Identity = 74/245 (30.20%), Postives = 124/245 (50.61%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T DEL +WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGKIVSKIDGDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. UniProt/SwissProt
Match: sp|O35887|CALU_MOUSE (Calumenin OS=Mus musculus OX=10090 GN=Calu PE=1 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 2.917e-21
Identity = 74/245 (30.20%), Postives = 123/245 (50.20%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T DEL  WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGKIVSKIDDDKDGFVTVDELKGWIKFAQKRWIHEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNADEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. UniProt/SwissProt
Match: sp|Q5RDD8|CALU_PONAB (Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 4.339e-21
Identity = 74/245 (30.20%), Postives = 124/245 (50.61%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID DK+  +T DEL +WI     + + +  +     +D+N+D  ++W E    TYGY       +L+  DP D  NY Q +V D  RFK AD D D   T+EE+ + L+P     +  + + + +   DK+ D  ++ +E+   +Y +         +      F+++ D N+D K+  +E + W  P     A  EA  L+ +SD N D  LT +EI+ K + +  SQAT +G
Sbjct:   69 SKERLGKIVSKIDDDKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGY-------VLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. TrEMBL
Match: A0A267GP25 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024316g1 PE=4 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 2.142e-30
Identity = 88/247 (35.63%), Postives = 131/247 (53.04%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKR-AKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRD 963
            S  +L  L+ ++DSD N  I K+EL  WI++ S K LD+  A S +  +D N D K+TW E L   YGY  E +++    K P      Q + +D+ +F+ AD + DG L  EEY    +P     +  V +DK +  +DK++D  ++E+E    F   +++           F +YD N D KL  DE++ W  P    SA  EA  L+++SD + D VL+ DEIL +   W  SQAT YG H+ D
Sbjct:   73 SKKRLAVLVGRMDSDGNGKIDKNELTNWIVN-SFKKLDREDAASKMEEHDENDDKKVTWPEYLHKAYGYKVEELKEFEKDKSPDMLSFLQMLEEDKRKFQLADHNHDGSLDLEEYTFFTHPHNYDIMAPVEIDKTMKDYDKNSDGFIDEKE----FMGEDKMDPETSKAESEHFKQYDKNGDGKLDRDEVKAWTMPKFEDSAATEAEHLIKESDKDKDGVLSKDEILDQHELWVGSQATDYGKHLDD 314          
BLAST of Reticulocalbin-2 vs. TrEMBL
Match: A0A183AI51 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS6636 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 6.901e-30
Identity = 81/255 (31.76%), Postives = 137/255 (53.73%), Query Frame = 1
Query:  232 TKLRELLVKIDSDKNEIITKDELVEWII-SLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF----KLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHI----RDEL 969
             +L  L+ ++D+DK+ +I+K+EL+ WI+ S  L DL+  A+  +  +D N D  ++W E L   YGY+E+ +E +  ++ P  +   Q +  ++ +F +AD D++G L + EY +   P   P +    +   L   D+D D  ++E+E+    +   +  +++    L+  +  F +YD N D KL  +EL  W  P    +A  EA  L+ ++D N D  L+ DEIL +   W  SQAT YG H+    RDEL
Sbjct:   67 ARLARLIERMDADKDGVISKEELINWIVHSFELLDLES-ARQRLKSHDENADGLVSWAEFLHKVYGYSEQEMEDLKKTEGPEIQAFVQSVQQEKVKFDSADLDKNGLLNETEYAAFEYPHNYPHMAPYEIIHTLKDFDRDKDGLISEEEYLQDIRFRSFSADKMQPEALVIEKANFKRYDKNGDGKLDREELVDWVTPGFRRTATSEAEHLLMETDQNKDGKLSKDEILKQHELWVGSQATDYGRHLEQMPRDEL 320          
BLAST of Reticulocalbin-2 vs. TrEMBL
Match: A0A0B7B4B9 (Uncharacterized protein OS=Arion vulgaris OX=1028688 GN=ORF161933 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 1.312e-29
Identity = 93/252 (36.90%), Postives = 134/252 (53.17%), Query Frame = 1
Query:  229 VTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKR-AKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDN-YQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTH---IRDEL 969
            + +LR L    D++ N+II   EL EW++  S + LD+  A   +   D N D K+T+ E+L   YGY+EE +E +    +  D  N +Q I DDR+RF  AD ++DGFL + EY +   P   P +  V M++ +   DKD D  ++ QEF     I E   E   L     F + D +KD KL+ +EL  W  PD N SA  E   L+   D + D  L+ DEI+SK +E+  S AT YG     ++DEL
Sbjct:   66 IKRLRNLAKSHDANNNDIIEAHELKEWVLE-SFRMLDREEAMEKLEDEDENNDGKVTFDEILHKQYGYSEEDLEDVQKEAEDDDGANVFQLITDDRNRFAVADVNKDGFLDETEYIAYFQPYDFPYMYDVEMERAMKDLDKDTDGYISIQEF-----IGENSDEETRLSEMTNFEELDKDKDGKLTPEELRPWALPDNNESAVEEVEHLLSICDDDKDGHLSIDEIVSKEDEFLRSTATDYGRTLHFVKDEL 311          
BLAST of Reticulocalbin-2 vs. TrEMBL
Match: A0A074ZLX5 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_04760 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.202e-28
Identity = 83/251 (33.07%), Postives = 137/251 (54.58%), Query Frame = 1
Query:  232 TKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLD-KRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTH----IRDEL 969
            T+L +L  K+D+++N  + K+EL++WI+  S  +LD + AK  ++ YD ++D  +TW E     YGY+   +E++        +   + I +++ +FK+AD D++G L   E+ +  +P   P +    +   L   D DND  +++QE   Y    +   E   +E EN F +YD N D +L  +E++ W  P    +A  EA  L  ++D NGDK L+ +E+L++   W  SQAT YG H    IRDEL
Sbjct:   88 TRLGQLFEKMDANQNGNLDKNELIDWIVQ-SFTNLDLESAKIKLNDYDADQDGTLTWEEYTTRVYGYSSTELEQLAKDSSNETQAFLRSIEEEKIKFKSADLDQNGHLNATEFTAFEHPHNYPHMAPYEIIHTLRDFDTDNDGFISQQE---YLADDKMHREAFKIELEN-FKRYDTNGDGRLDQEEMKHWVTPGFQRTATEEAEHLFSETDANGDKELSKEEVLAQHELWVGSQATDYGRHLEHSIRDEL 333          
BLAST of Reticulocalbin-2 vs. TrEMBL
Match: A0A2C9JHC9 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106064556 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 7.601e-28
Identity = 92/252 (36.51%), Postives = 134/252 (53.17%), Query Frame = 1
Query:  229 VTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKR-AKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILN-SKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTH---IRDEL 969
            + +LR L    DS+ N+II  DEL EWI+  S + LD+  A   +   D N D K+T+ E+L   +GY EE +  + N  +   D D  + I DD+ RF AAD D+DG L+QEEY +   P   P + +V M++ +   DKD D  ++ +EF     I E + +   +     F + D +KD KL+ DEL  W  PD    A  EA  L+   D + D  L+ +EI++K  E+  S AT YG     +RDEL
Sbjct:   21 IKRLRNLAKSHDSNNNDIIELDELKEWILQ-SFRMLDREEAMEKLQEEDENGDKKVTFAEILGKRHGYGEEDLPLLENYERGDEDVDVLELINDDKKRFLAADLDKDGALSQEEYVAYFQPYDFPHMFQVEMERSMHDIDKDADGFISMEEF-----IGENVDDETRISEMTNFEELDKDKDGKLTPDELRPWALPDNEDVAKEEAEHLLNMCDLDRDGKLSIEEIVNKEEEFIGSSATDYGRTLHFVRDEL 266          
BLAST of Reticulocalbin-2 vs. Ensembl Cavefish
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:103039743])

HSP 1 Score: 101.293 bits (251), Expect = 4.147e-24
Identity = 80/255 (31.37%), Postives = 134/255 (52.55%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  +L +++ +ID+D N  IT +EL  WI  +  + + +      S YD+NKDNKI+W E  + TYGY   + E+    +D  D+ +++  +  D  RFK AD D D    +EE+ + L+P     + ++ + + +   DK+ D  ++E E+   ++ +         +    + F  + D NKD K+ ++E+  W  P     A  EA  L+ +SDT+ D++LT +EIL   N +  SQAT+YG   T   DEL
Sbjct:   70 SRDRLGKIVDRIDADGNGYITTEELKAWIKRVQKRYVYENVAKVWSDYDLNKDNKISWDEYKQATYGYYLANPEEF---EDATDQFSFKKMLPRDERRFKLADLDGDLAAEREEFTAFLHPEEFDHMKEIVVLETMEDIDKNGDGHVDEDEYIADMFAHEEGGPEPDWVKTERDQFSDFRDLNKDGKMDLEEIRHWILPQDYDHAQAEARHLLLESDTDKDQMLTKEEILENWNMFVGSQATNYGEDLTRNHDEL 321          
BLAST of Reticulocalbin-2 vs. Ensembl Cavefish
Match: rcn3 (reticulocalbin 3 [Source:NCBI gene;Acc:103045651])

HSP 1 Score: 92.4337 bits (228), Expect = 6.461e-21
Identity = 76/256 (29.69%), Postives = 128/256 (50.00%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYG-YNEESVEKILNSKDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            S  KL +++ +ID+DK+  ++  EL  WI     + +++        YD +KD KI W E   TTYG Y ++    + N      +++Y+  ++ D  RFK AD D DG  ++EE+ + L+P     +  + + + +   DK+ D ++N +E+   +Y     E     +   +  F ++ D NKD  L   E+ +W  P     A+ EA  L+ ++D + D  +T  EIL   N +  SQAT+YG   T   DEL
Sbjct:   64 SKAKLGKIVDRIDTDKDGFVSHAELHHWIKHRQRRYIEENVDKHWKEYDQDKDGKIAWTEYKNTTYGFYLDDEFSDVEN------RESYKAMLMRDERRFKVADKDGDGIASREEFTAFLHPEEFSYMKDLVVQETVEDIDKNGDGKINLEEYIGDMYTSEDGENEPEWVNTEKKQFSEFRDVNKDGFLDASEVAQWILPGDVDHADIEAKHLIHETDRDNDDKMTKKEILENWNMFVGSQATNYGEDLTRKHDEL 313          
BLAST of Reticulocalbin-2 vs. Ensembl Cavefish
Match: calua (calumenin [Source:NCBI gene;Acc:103043492])

HSP 1 Score: 83.5741 bits (205), Expect = 7.493e-18
Identity = 74/249 (29.72%), Postives = 124/249 (49.80%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLK----DLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KID D++  +T+ EL EWI     K    ++D++ K     +D+N D  I+W E    TYG         L+  +P D  NY Q +  D  RFK AD + D    +EE+ + L+P     + ++ + + +   DK+ D  ++ +E+   +Y +  E      +      F ++ D NKD K+  +E   W  P     A  EA  L+ +SD+N D  L+ +EIL+K + +  SQAT +G
Sbjct:   69 SKDRLGKIVEKIDDDQDGFVTEAELKEWIKKAQKKYIYDNVDRQWKD----FDMNNDGMISWDEYKNVTYG-------TYLDDPEPDDGYNYKQMMARDERRFKMADRNGDSIADKEEFTAFLHPEEYEHMKEIVVLETMEDIDKNGDGFIDLEEYIGDMYNHEDEMDEPEWVATEREQFSEFRDKNKDGKMDKEETMDWILPADYDHAEAEAKHLVYESDSNKDGKLSKEEILNKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Cavefish
Match: rcn2 (reticulocalbin 2 [Source:NCBI gene;Acc:103045530])

HSP 1 Score: 82.8037 bits (203), Expect = 1.350e-17
Identity = 83/294 (28.23%), Postives = 144/294 (48.98%), Query Frame = 1
Query:  109 LLSLKCSLVFLTLLYFVLSEEEILPN---FSEQSITDNFGSYSVTKLR----ELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFI-VDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLK--YDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            LL+L C+       +  LSE  I  N   F +    D     S ++ R    E++ +ID+D ++ +T +E+  WI  +  +     A+     +DIN D  +TW E     +G+  E  E  +  +DP ++++ +F+ + ++ RF  AD+D    L   E+ +  +PS    +    ++ +LS +D D D  ++  EF      +E    +Q    E +  K  YD NKD KL+ DE  +W AP+   SA  EA  L+++ D +GD+ L+  EIL   + + +S+ T YG  + 
Sbjct:   10 LLALSCTA------HQHLSEHHIQHNSNAFLDDEDKDEIKKLSPSEQRGKMVEIVKRIDTDSDKFLTPEEITVWIQRVYKRYAMDDAQERFPGFDINSDGLVTWDEYNMVMHGHTVEVDENAV-LEDP-EEESLRFLHLKEKRRFDFADTDGTSGLNVTEFLAFTHPSEVDHMSDFAIEDVLSEYDLDKDGFISLSEFIGDLRASESDEPSQWEAEETVRFKDLYDQNKDGKLNRDEQLRWVAPNSYGSAREEAVHLIKEMDQDGDERLSETEILKNQDIFMNSEVTDYGRQLH 295          
BLAST of Reticulocalbin-2 vs. Ensembl Cavefish
Match: calub (calumenin-B [Source:NCBI gene;Acc:103027690])

HSP 1 Score: 82.4185 bits (202), Expect = 1.989e-17
Identity = 73/249 (29.32%), Postives = 122/249 (49.00%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLK----DLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  ++ KID D +  +T +EL +WI     +    D+D++ +S    +D+N D  ++W E    TYGY       IL+  DP D  NY Q +  D  RFK AD+D +    +EE+ + L+P     +  + + + +   DK+ D  ++  E+   +Y    +      +      F ++ D NKD ++  +E   W  P     A  EA  L+ +SDT+ D +LT  EI+ K + +  SQAT +G
Sbjct:   69 SKERLGLIVDKIDEDHDAHVTVEELKKWIKHAQKRWIYEDVDRQWQS----HDLNADGYVSWEEYKNATYGY-------ILDDSDPDDGFNYRQMMARDERRFKMADTDGNLQADKEEFTAFLHPEEYDYMKDIVVLETMEDIDKNGDGFIDLDEYIGDMYSQDGDPTEPEWVRTEREQFTEFRDKNKDGRMDKEETRDWILPSDYDHAEAEAKHLLYESDTDKDGMLTKQEIVDKYDLFVGSQATDFG 306          
BLAST of Reticulocalbin-2 vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 94.7449 bits (234), Expect = 4.954e-23
Identity = 69/238 (28.99%), Postives = 118/238 (49.58%), Query Frame = 1
Query:  247 LLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIV-DDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLK-YDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            ++ +ID + + ++T+ EL EWI     + + + A+   S +D++ D  + W E    TY Y        L  +DP D D Y+ +V  D  RF+AAD D DG  ++ EY + L+P   P +  + + + +   DK+ D  +N  E+   +Y   +EE     +      F +  D + D  +  DE+  W  PD    A  E+  L+ + D + D  LT +EI++K + +  SQAT +G
Sbjct:    2 IVTRIDENGDGLVTETELTEWIRHAQRRWVLEEAERQWSSHDLDGDGYVNWAEYKNATYSY--------LAEEDPEDPDGYRAMVLRDERRFQAADVDGDGRASRNEYAAFLHPEEHPHMRNLVVTETIEDIDKNGDGFINVDEYIGDMYSEESEEGDSEWVESERKQFAELRDTDGDGLMGHDEVAAWILPDDYDQAEAESKHLIHECDDDQDGNLTREEIIAKYDLFVGSQATDFG 231          
BLAST of Reticulocalbin-2 vs. Ensembl Sea Lamprey
Match: CALU (calumenin [Source:HGNC Symbol;Acc:HGNC:1458])

HSP 1 Score: 90.8929 bits (224), Expect = 3.895e-21
Identity = 73/247 (29.55%), Postives = 121/247 (48.99%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY---QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDS--QLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ KIDSD + ++T+DE+  WI     + + +      + +DI+ D  ++W E    TYG+   S         PI++D +   Q +  D  RFKAAD D DG  ++EE+ + L+P     +  + + + +   DK+ D    LNE    +Y    E      +      F  + D NKD K+  +E   W  P     A  EA  L+ +SD++ D  L+ +EIL K + +  SQAT++G
Sbjct:   71 SKERLGKIVEKIDSDGDGLVTQDEMTMWIKHTQRRWVYEDVDRQWTGHDIDGDGSVSWEEYRNATYGFLAVS---------PIEEDGFNYKQMLGRDERRFKAADVDGDGQASKEEFTAFLHPEEFEHMKDIIVTETMEDIDKNGDGFIDLNEYIGDMYNQAGESEEPEWVKTERQQFTDFRDTNKDGKMDKEETMAWILPSDYDHAEAEARHLIYESDSDKDGKLSKEEILDKHDLFVGSQATNFG 308          
BLAST of Reticulocalbin-2 vs. Ensembl Sea Lamprey
Match: sdf4 (stromal cell derived factor 4 [Source:NCBI gene;Acc:406360])

HSP 1 Score: 57.3806 bits (137), Expect = 8.591e-10
Identity = 67/255 (26.27%), Postives = 131/255 (51.37%), Query Frame = 1
Query:  250 LVKIDSDKNEIITKDELVEWIISLSLKDLDK---RAKSDISFYDINKDNKITWIE-MLR--TTYGYNEESV-EKILNSKD-PIDKDNYQFIVDDRDRF-KAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXX------XXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDE 966
            LV++D D+++ I+  EL EWI+  + + L +     K      D + D  +TW E  LR   + G++E+ + E+I + ++  ID++  + + + ++R+ +A D+ +D  L ++E+ S  +P  S  +L   + +I+   D+D D +L+  EF      T E  + Q      + E    F++  D NKD  ++  ELE++  P  + +A  EA  ++  +D + D  L+ DE+L     +  S+   Y  ++ +E
Sbjct:    6 LVRVDKDRDKRISAVELQEWIVEKTREHLQQALDENKPHFRTLDPDGDGLVTWDEYRLRYLVSKGFDEKDIAERIRDKQELKIDEETEELMDNLKERWNQADDAPQDQKLNEKEFLSFQHPEHSRSMLSYMVQEIIHDLDQDKDQRLSLSEFASLPLGTVENQKEQDIDDDWVRERRKEFIEVIDSNKDGFITRSELEEYMDPTSSHNALSEAKQMISVADEDEDAQLSLDEVLRYSEYFTGSKLVDYARNVHEE 260          
BLAST of Reticulocalbin-2 vs. Ensembl Nematostella
Match: EDO35844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKE6])

HSP 1 Score: 104.375 bits (259), Expect = 1.031e-25
Identity = 74/240 (30.83%), Postives = 127/240 (52.92%), Query Frame = 1
Query:  235 KLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIF-LKYDFNKDRKLSIDELEKWYAPDL-NISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            +LR L+ ++D++K+  +T +EL +W+  +  K   +   +D+   D N+D K+ W E  + TYG   E  E++            +F+  D+ RF AAD+++DGFLT+EE    L+P  SP++ +V++ + +   D+D D  ++ +EF   +          + +  N F  +YD NKD KL  +E+  W  P+  ++ A  EA  L+  +D N D  LT +EI      +  S+AT YG
Sbjct:   72 RLRALIREVDNNKDGAVTTEELTDWVKGVFKKRSMEGVDNDLKEKDANEDGKVDWNEYSKGTYGDQTEDDEEM-----------KEFLRRDKRRFDAADTNKDGFLTREEMAIFLHPESSPEMSEVHILETIEDIDRDKDGLISLKEFLGEYEEEPGEVPDWVKDETNRFNEEYDKNKDGKLDKEEVRLWILPETDHLMAAEEAKHLVSSADDNSDGKLTIEEIEKNYAVFVGSEATDYG 300          
BLAST of Reticulocalbin-2 vs. Ensembl Nematostella
Match: EDO42789 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0S7])

HSP 1 Score: 98.2117 bits (243), Expect = 7.875e-24
Identity = 81/255 (31.76%), Postives = 125/255 (49.02%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDI--NKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLY-----FYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
            +  +LR L+ +ID +K+  + + EL  WI    +K  +     D  F D+  N DN  TW E +  TYG+ +E + K     +  D     +I DDR ++K AD D+D  LT+EEY+   +P     ++     K++   DKD D  L+ QE+  +     F+I ++    +         K+D NKD KL + E+E W  P     A  EA  L+E +D NGD  L+ DEI++    +  S AT +G    DE 
Sbjct:   28 ARRRLRMLVPRIDINKDGFVEEAELEIWI-RHKMKKWEVEEDVDAIFRDMDFNNDNHATWKEFMMRTYGFTDEDINKKWERNNLKD-----YIEDDRRKWKYADQDKDSRLTREEYEYFHHPKEHEVMIPYIAMKVMLEGDKDKDGFLSLQEYLDWLEMPDFHIMDKRDFEE---------KHDQNKDGKLDLKEVEDWRRPKNFNKALEEAQHLIEHADLNGDGKLSADEIVTSHEFFAGSYATQFGQTFHDEF 267          
BLAST of Reticulocalbin-2 vs. Ensembl Nematostella
Match: EDO31169 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYP9])

HSP 1 Score: 92.4337 bits (228), Expect = 1.208e-21
Identity = 80/252 (31.75%), Postives = 132/252 (52.38%), Query Frame = 1
Query:  235 KLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVD-DRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFN------KDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
            +LREL  KID D+++ I+  ELVEWI     K   K ++S +   D NKD K++W E +   Y   +   EK ++++   +KD+ + +   D  R+K AD D D  LT +E +  ++P  +P++  V + + +   D D D +++  E+     +   + E      ++  LK DFN      KD  L+ +EL+ W  P  + S+  EA  LM + D N D  LT DEI+ +   +  S+AT+YG  ++ EL
Sbjct:   27 RLRELFPKIDVDQDQKISLKELVEWIDVNMKKHTRKSSESRMEQMDKNKDGKVSWEEYVNVEY---DPKNEKGMSNE---NKDHLKEMKKRDEKRWKHADMDNDNLLTIDELQMFIHPEETPRMTSVLVQENMEMLDSDKDGKISFAEYAGKLSVMWRMREDNQDSLKS--LKDDFNNDLDKDKDGSLNKEELKSWIFPSGSPSSG-EAEHLMTEVDKNKDNFLTVDEIMERYELFAGSRATNYGNMLKKEL 269          
BLAST of Reticulocalbin-2 vs. Ensembl Nematostella
Match: EDO28554 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T654])

HSP 1 Score: 77.0258 bits (188), Expect = 1.081e-16
Identity = 42/134 (31.34%), Postives = 77/134 (57.46%), Query Frame = 1
Query:  235 KLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSG 636
            +LR L+ ++D++K+  +T +EL +W+  +  K   +   +D+   D N+D K+ W E  + TYG   E  E++            +F+  D+ RF AAD+++DGFLT+EE    L+P  SP++ +V++ + + G
Sbjct:   72 RLRALIREVDNNKDGAVTTEELTDWVKGVFKKRSMEGVDNDLKEKDANEDGKVDWNEYSKGTYGDQTEDDEEM-----------KEFLRRDKRRFDAADTNKDGFLTREEMAIFLHPESSPEMSEVHILETIEG 194          
BLAST of Reticulocalbin-2 vs. Ensembl Nematostella
Match: EDO49919 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFA2])

HSP 1 Score: 77.411 bits (189), Expect = 1.681e-16
Identity = 62/222 (27.93%), Postives = 110/222 (49.55%), Query Frame = 1
Query:  244 ELLVK--IDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEIL 903
            E LVK  +D DK+  +T+DEL   +++ S K       S ++F+D NKD K++W E  +  + + E   +         DK   + + +D  +FK AD + DG L   EY +  +P    ++    +   L  HD D D  +++ E+   F  ++   + ++   ++    +D NKD KL   E+  W  PD ++ A  E   +++++D N D  L+ +EIL
Sbjct:   23 EQLVKSEVDLDKDGFVTEDELRLRLLNTSRKHRKTEVNSTVTFHDDNKDGKVSWEEFKKAHFTHTEGKED---------DKATKEQMAEDEAKFKYADVNGDGMLDLHEYVTFYHPGDDERMSAWVIQDTLKKHDTDKDGMISKSEYMATF--SDANNDAKIELEKDFDTSFDKNKDGKLDQTEIRHWLFPDDDM-AKEEPAHMIKEADDNKDGKLSMEEIL 232          
BLAST of Reticulocalbin-2 vs. Ensembl Medaka
Match: rcn1 (reticulocalbin 1 [Source:NCBI gene;Acc:101175141])

HSP 1 Score: 102.834 bits (255), Expect = 2.097e-24
Identity = 93/332 (28.01%), Postives = 148/332 (44.58%), Query Frame = 1
Query:   73 ITFLKIKIMKMNLLSLKCSLVFLTLLYFVLSEEEIL--PNFSEQSITDN----------------------FGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQF---IVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG---THIRDEL 969
            +  L  +  +  + +LK      T     + +E +L  P  S Q+  DN                          S  +L +++ +ID D +  IT  EL +WI  +  + + +      + YD+NKDN+I+W E  + TYGY   + E+  +     DKD + F   +  D  RFKAAD + D    +EE+ S L+P     +  + + + L   DK+ D  ++E E+   ++ +         +      F  + D NKD K+  DE+  W  P     A  EA  L+ +SD + D+ LT DEIL   N +  SQAT+YG   T   DEL
Sbjct:   26 LVLLSARTSRSGIAALKTGSQLKTKAKPTIRKERVLRDPELSRQAHEDNKSFQYDHEAFLGKEEAKTFDQLTPEESRDRLGKIVERIDGDGDGYITTAELKDWIKRVQKRYVYENVAKVWTDYDLNKDNRISWDEYKQATYGYYLANPEEFQD-----DKDQFSFKKMLPRDERRFKAADLNGDNTADKEEFTSFLHPEEFDHMKDIVVQETLEDIDKNGDGYVDEDEYIADMFSHEDGGPEPDWVKTEREQFSDFRDLNKDGKMDKDEIRHWIMPQDYDHAQAEARHLVYESDQDKDQKLTKDEILENWNMFVGSQATNYGEDLTRSHDEL 352          
BLAST of Reticulocalbin-2 vs. Ensembl Medaka
Match: rcn3 (reticulocalbin-1 [Source:NCBI gene;Acc:101175408])

HSP 1 Score: 92.4337 bits (228), Expect = 6.678e-21
Identity = 75/247 (30.36%), Postives = 120/247 (48.58%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNS--KDPIDKDNYQ-FIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L +++ +ID+DK+  I+  EL  WI     + ++         YD N+D++I W E   TTYGY        L     D  DK  Y+  +  D  RF+AAD D DG  T+ E+ + L+P     +  V + + L   DK+ D +++  E+   ++     E     +   +  F ++ D NKD  L   E+  W  P     A+ EA  L+ ++DT+ D  +T  EIL+  N +  SQAT+YG
Sbjct:   69 SKARLAKIVERIDTDKDGFISHAELHHWIKHRQRRYIEDNVNKHWKDYDKNRDDQIAWEEYKNTTYGY-------YLGEDFDDVEDKATYKSMLARDERRFRAADRDADGIATRSEFTAFLHPEEFDHMKDVVVQETLEDIDKNKDGKIDINEYIGDMFTPEDGETEPDWVHTEKKHFSEFRDSNKDGYLDAGEVAHWILPGEVDHADNEAKHLIHETDTDKDGKVTKKEILANWNMFVGSQATNYG 308          
BLAST of Reticulocalbin-2 vs. Ensembl Medaka
Match: calub (calumenin [Source:NCBI gene;Acc:101165303])

HSP 1 Score: 83.9593 bits (206), Expect = 5.655e-18
Identity = 74/249 (29.72%), Postives = 121/249 (48.59%), Query Frame = 1
Query:  226 SVTKLRELLVKIDSDKNEIITKDELVEWIISLSLK----DLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNY-QFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF--KLYFYIXXXXXXXXXXXXXNIFLKY-DFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYG 948
            S  +L  L+ +ID DK+  +T +E+  WI     +    D+D++ KS    +D+N D  ++W E    TYGY       +L+ +DP D  +Y Q +  D  RFK AD D D    +EE+ + L+P     +  + + + +   DK+ D  ++  E+   +Y    E      +      F ++ D NKD K+  +E + W  P     A  EA  L+ +SD + D  LT  EI+ K + +  SQAT +G
Sbjct:   72 SKERLGMLVDRIDEDKDGYVTAEEMKRWIKDAQKRWIYDDVDRQWKS----HDLNGDGFVSWEEYKNATYGY-------VLDDQDPDDGFSYKQMMARDERRFKMADQDNDMKANKEEFTAFLHPEEYEHMKDIVVLETMEDIDKNGDGFIDLDEYIGDMYNQDGETTEPEWVKTEREQFTEFRDKNKDGKMDKEETKDWILPSDYDHAEAEAKHLVYESDVDKDGRLTKAEIVDKYDLFVGSQATDFG 309          
BLAST of Reticulocalbin-2 vs. Ensembl Medaka
Match: pstpip1a (proline-serine-threonine phosphatase-interacting protein 1 [Source:NCBI gene;Acc:101156493])

HSP 1 Score: 69.3218 bits (168), Expect = 6.307e-13
Identity = 64/247 (25.91%), Postives = 119/247 (48.18%), Query Frame = 1
Query:  235 KLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTT----YGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLK-YDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIR 960
            KL E++ KID++ + ++  +E+  WI  +  K   + A+     +D+NKD  +TW E         + ++E +V      +DP      Q  + ++ RF  AD D+   L+  E+ +  +PS   ++    +  +L+ +D D D  ++  EF       +       +E    F + YD +KD  L+ DE  +W AP+   SA  EA  L+ + D +GD+ ++  E+L   + + +S+ T YG  + 
Sbjct:   63 KLLEIVKKIDTNGDNLLGAEEITLWIQHVYRKYALEDAEERFPEFDLNKDGVLTWEEYNTVAHDQLFTFDESTV-----LEDPEQDSLRQLHLKEKKRFDFADIDDTPGLSVSEFLAFTHPSEVDRMADFTIQDVLTEYDTDKDGFISLSEFIGDVRGEDNSPSKWEIEETVRFKELYDQDKDGNLNRDEQLRWIAPNSYGSAREEALHLINEMDQDGDEQISVAEVLKNQDTFMNSEVTDYGRQLH 304          
BLAST of Reticulocalbin-2 vs. Ensembl Medaka
Match: sdf4 (stromal cell derived factor 4 [Source:NCBI gene;Acc:101159824])

HSP 1 Score: 63.1586 bits (152), Expect = 8.741e-11
Identity = 68/261 (26.05%), Postives = 130/261 (49.81%), Query Frame = 1
Query:  235 KLRELLVKIDSDKNEIITKDELVEWIISLSLKD-LDKRAKSDISF--YDINKDNKITWIE---MLRTTYGYNEESV-EKILNSKD-PIDKDNYQFIVDDRDR-FKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEF-KLYFYIXXXXXXXXXXXXXNIFLKYDF------NKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
            KL E+  K+D++K+  ++  E+  WI+  + +  L+ + ++  SF   D + D  +TW E       + G+NE+ V EKI  ++D  +D++  + +   +DR F+A +   D  L ++E+ S L+P  S  +L+  + +I+   D+D D +L   EF  L F   E      + +      K +F      N+D  +++ ELE++  P    +A  EA  ++  +D N ++ L  +EIL     +  S+   Y  ++ +E 
Sbjct:   97 KLIEIFTKVDANKDRSVSAKEMQRWIMEKTEEHFLEAQTENKNSFRAVDPDGDGHVTWDEYRVKFLASKGFNEKEVAEKIKKNEDLKLDEETQEVLESLKDRWFQADNHPTDQLLNEQEFLSFLHPEHSRGMLQYMVKEIVRDLDQDGDKKLTVSEFISLPFGTVENQHAQDVDDDWVKERKKEFEEVIDANRDGIVTMAELEEYMDPMNEHNALNEAKQMIAVADENQNRHLELEEILRYSEYFTGSKLMDYARNVHEEF 357          
BLAST of Reticulocalbin-2 vs. Planmine SMEST
Match: SMESG000028739.1 (SMESG000028739.1)

HSP 1 Score: 539.65 bits (1389), Expect = 0.000e+0
Identity = 286/291 (98.28%), Postives = 289/291 (99.31%), Query Frame = 1
Query:   97 MKMNLLSLKCSLVFLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
            MKMNLLSLKCSL FLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD+KITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLT EEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYITEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDK+LTFDEILSKLNEWKDSQATSYG+HIRDEL
Sbjct:    1 MKMNLLSLKCSLAFLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDSKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTHEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYITEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKMLTFDEILSKLNEWKDSQATSYGSHIRDEL 291          
BLAST of Reticulocalbin-2 vs. Planmine SMEST
Match: SMESG000028739.1 (SMESG000028739.1)

HSP 1 Score: 535.798 bits (1379), Expect = 0.000e+0
Identity = 284/289 (98.27%), Postives = 287/289 (99.31%), Query Frame = 1
Query:  103 MNLLSLKCSLVFLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
            MNLLSLKCSL FLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKD+KITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLT EEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYITEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDK+LTFDEILSKLNEWKDSQATSYG+HIRDEL
Sbjct:    1 MNLLSLKCSLAFLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDSKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTHEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYITEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKMLTFDEILSKLNEWKDSQATSYGSHIRDEL 289          
BLAST of Reticulocalbin-2 vs. Planmine SMEST
Match: SMESG000028234.1 (SMESG000028234.1)

HSP 1 Score: 118.242 bits (295), Expect = 1.385e-30
Identity = 81/251 (32.27%), Postives = 138/251 (54.98%), Query Frame = 1
Query:  217 GSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
             +++   L +L  KID++++  +TKDEL+ WI+  + + L + AK ++   D N DN +TW E ++  YG    + +K+ +  + I     QF  DD+ RF  AD+D++  L+ EE K+ L P    ++L   + + L  +DK+ D  ++  EF    Y + +LT  Q      +F KYD + + +LS +E++ W  PD+   A  E+  L+  SD N D+ L+ +EIL     W  S+AT++G   +DEL
Sbjct:   55 SNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEI----IQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDEL 301          
BLAST of Reticulocalbin-2 vs. Planmine SMEST
Match: SMESG000028234.1 (SMESG000028234.1)

HSP 1 Score: 117.472 bits (293), Expect = 1.907e-30
Identity = 81/251 (32.27%), Postives = 138/251 (54.98%), Query Frame = 1
Query:  217 GSYSVTKLRELLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL 969
             +++   L +L  KID++++  +TKDEL+ WI+  + + L + AK ++   D N DN +TW E ++  YG    + +K+ +  + I     QF  DD+ RF  AD+D++  L+ EE K+ L P    ++L   + + L  +DK+ D  ++  EF    Y + +LT  Q      +F KYD + + +LS +E++ W  PD+   A  E+  L+  SD N D+ L+ +EIL     W  S+AT++G   +DEL
Sbjct:   45 SNHTKQGLGKLFHKIDANQDLFVTKDELIRWIVQYNEEILMREAKFELKRIDKNNDNFVTWEEFMKEHYGNLSLNEDKVNSEHNEI----IQFFKDDKYRFTIADADKNERLSIEELKAFLRPHNHVEMLDYEVYRALIEYDKNKDKVIDRTEFIQTQYGSNKLTLQQKSYVNKVFDKYDTDHNNQLSENEMKVWLKPDIIQFAQTESQYLLNVSDLNKDEKLSKEEILENFGHWVSSKATNFGADFKDEL 291          
BLAST of Reticulocalbin-2 vs. Planmine SMEST
Match: SMESG000054251.1 (SMESG000054251.1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.347e-16
Identity = 59/223 (26.46%), Postives = 115/223 (51.57%), Query Frame = 1
Query:  235 KLRELLVKIDSDKNEIITKDELVEWIISLSLKDLD-KRAKSDISFYDINKDNKITWIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDGFLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYIXXXXXXXXXXXXXNIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANSLMEKSDTNGDKVLTFDEI 900
            +L  L+ KID++ +  + + EL +WI+  S ++LD + A + +   D N D  +TW E +  TYG++E+ +++++ +     K   + ++ D+ +F+ AD + D  L   E+    +P   P++    ++K L   DK+ D  ++ +E    F + E++ +      +  F +YD +K+  L   E+  W  P    +A  E   L  ++D N DK L+ +EI
Sbjct:   80 RLGLLVDKIDTNHDSYVNQTELFKWIMQ-SFRNLDLEDATTKMQDVDENNDKMLTWAEYVSKTYGHSEKEIDEMIKTGSTDMKGFIEGVLMDKKKFQLADRNNDSMLDVPEFAKFSHPHNYPEMSTFELEKTLEDFDKNKDGGVDLKE----FVVDEKMDKETEEHEKKSFAEYDKDKNGLLQGSEITDWVMPGFEKTAVSEVEHLFNETDVNKDKNLSKEEI 297          
The following BLAST results are available for this feature:
BLAST of Reticulocalbin-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALU5.028e-2230.20calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU5.645e-2230.20calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU4.018e-2030.20calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU4.047e-2030.20calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
RCN31.703e-1828.02reticulocalbin 3 [Source:HGNC Symbol;Acc:HGNC:2114... [more]
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BLAST of Reticulocalbin-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
calu-27.493e-2030.35CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-11.321e-924.81CALUmenin (Calcium-binding protein) homolog [Sour... [more]
calu-11.321e-924.81CALUmenin (Calcium-binding protein) homolog [Sour... [more]
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BLAST of Reticulocalbin-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG316508.099e-2331.37gene:FBgn0031673 transcript:FBtr0301907[more]
CG316508.099e-2331.37gene:FBgn0031673 transcript:FBtr0301905[more]
CG316508.099e-2331.37gene:FBgn0031673 transcript:FBtr0079033[more]
CG316508.099e-2331.37gene:FBgn0031673 transcript:FBtr0079034[more]
CG316508.099e-2331.37gene:FBgn0031673 transcript:FBtr0301906[more]
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BLAST of Reticulocalbin-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn12.773e-2330.59reticulocalbin 1, EF-hand calcium binding domain [... [more]
rcn31.578e-2231.50reticulocalbin 3, EF-hand calcium binding domain [... [more]
rcn31.578e-2231.50reticulocalbin 3, EF-hand calcium binding domain [... [more]
rcn32.055e-2231.76reticulocalbin 3, EF-hand calcium binding domain [... [more]
rcn32.055e-2231.76reticulocalbin 3, EF-hand calcium binding domain [... [more]
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BLAST of Reticulocalbin-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fgf118.012e-2129.92fibroblast growth factor 11 [Source:Xenbase;Acc:XB... [more]
calu1.321e-2029.80calumenin [Source:NCBI gene;Acc:407965][more]
rcn38.693e-2030.92reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
rcn32.768e-1931.43reticulocalbin 3 [Source:NCBI gene;Acc:395023][more]
calu4.257e-1728.57calumenin [Source:NCBI gene;Acc:407965][more]
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BLAST of Reticulocalbin-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Calu4.169e-2230.20calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Calu2.500e-2030.20calumenin [Source:MGI Symbol;Acc:MGI:1097158][more]
Rcn32.903e-2029.18reticulocalbin 3, EF-hand calcium binding domain [... [more]
Rcn32.903e-2029.18reticulocalbin 3, EF-hand calcium binding domain [... [more]
Rcn12.357e-1930.77reticulocalbin 1 [Source:MGI Symbol;Acc:MGI:104559... [more]
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BLAST of Reticulocalbin-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O93434|RCN1_TAKRU3.020e-2432.05Reticulocalbin-1 OS=Takifugu rubripes OX=31033 GN=... [more]
sp|Q6XLQ7|CALU_RABIT1.303e-2130.20Calumenin OS=Oryctolagus cuniculus OX=9986 GN=CALU... [more]
sp|O43852|CALU_HUMAN2.415e-2130.20Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=... [more]
sp|O35887|CALU_MOUSE2.917e-2130.20Calumenin OS=Mus musculus OX=10090 GN=Calu PE=1 SV... [more]
sp|Q5RDD8|CALU_PONAB4.339e-2130.20Calumenin OS=Pongo abelii OX=9601 GN=CALU PE=2 SV=... [more]
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BLAST of Reticulocalbin-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GP252.142e-3035.63Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A183AI516.901e-3031.76Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A0B7B4B91.312e-2936.90Uncharacterized protein OS=Arion vulgaris OX=10286... [more]
A0A074ZLX51.202e-2833.07Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A2C9JHC97.601e-2836.51Uncharacterized protein OS=Biomphalaria glabrata O... [more]
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BLAST of Reticulocalbin-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn14.147e-2431.37reticulocalbin 1 [Source:NCBI gene;Acc:103039743][more]
rcn36.461e-2129.69reticulocalbin 3 [Source:NCBI gene;Acc:103045651][more]
calua7.493e-1829.72calumenin [Source:NCBI gene;Acc:103043492][more]
rcn21.350e-1728.23reticulocalbin 2 [Source:NCBI gene;Acc:103045530][more]
calub1.989e-1729.32calumenin-B [Source:NCBI gene;Acc:103027690][more]
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BLAST of Reticulocalbin-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CALU4.954e-2328.99calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
CALU3.895e-2129.55calumenin [Source:HGNC Symbol;Acc:HGNC:1458][more]
sdf48.591e-1026.27stromal cell derived factor 4 [Source:NCBI gene;Ac... [more]
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BLAST of Reticulocalbin-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Reticulocalbin-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO358441.031e-2530.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO427897.875e-2431.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO311691.208e-2131.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO285541.081e-1631.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO499191.681e-1627.93Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Reticulocalbin-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rcn12.097e-2428.01reticulocalbin 1 [Source:NCBI gene;Acc:101175141][more]
rcn36.678e-2130.36reticulocalbin-1 [Source:NCBI gene;Acc:101175408][more]
calub5.655e-1829.72calumenin [Source:NCBI gene;Acc:101165303][more]
pstpip1a6.307e-1325.91proline-serine-threonine phosphatase-interacting p... [more]
sdf48.741e-1126.05stromal cell derived factor 4 [Source:NCBI gene;Ac... [more]
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BLAST of Reticulocalbin-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000028739.10.000e+098.28SMESG000028739.1[more]
SMESG000028739.10.000e+098.27SMESG000028739.1[more]
SMESG000028234.11.385e-3032.27SMESG000028234.1[more]
SMESG000028234.11.907e-3032.27SMESG000028234.1[more]
SMESG000054251.16.347e-1626.46SMESG000054251.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014680 ID=SMED30014680|Name=Reticulocalbin-2|organism=Schmidtea mediterranea sexual|type=transcript|length=1043bp
TTTAGATAATTAATTTGTATTATTTTAGTAATAAAAATATTACTAAATCC
GTTATTTGGATATTCACTAGTTATAACATTTCTGAAAATTAAAATCATGA
AAATGAACTTATTGTCGTTAAAATGTTCATTAGTATTTTTAACGTTGTTA
TATTTCGTATTATCCGAAGAAGAAATCCTTCCTAATTTCAGTGAGCAATC
AATTACGGATAATTTTGGGTCATATTCTGTAACAAAATTACGTGAGCTTT
TAGTGAAAATCGATAGTGATAAAAATGAAATAATAACTAAAGATGAATTG
GTTGAATGGATTATTTCACTGTCTTTGAAGGACTTGGATAAAAGAGCAAA
ATCAGATATTAGTTTTTATGATATAAACAAGGATAACAAAATCACGTGGA
TTGAAATGCTTCGGACAACTTATGGATACAATGAAGAATCAGTTGAAAAA
ATTCTTAATTCAAAAGACCCAATTGATAAAGATAATTATCAATTTATAGT
AGATGATAGAGACCGATTTAAAGCAGCAGATTCTGATGAAGATGGATTTT
TAACTCAAGAAGAATACAAAAGTTTACTCAATCCGTCCGGAAGTCCAAAA
TTATTGAAAGTGTACATGGACAAAATTCTGTCCGGACATGACAAAGACAA
CGATAGTCAACTAAATGAACAAGAATTTAAGTTATATTTTTATATCACGG
AAGAATTAACTGAAACACAATTACTGGAATTTGAAAATATTTTCCTGAAG
TATGACTTTAATAAAGATAGAAAACTGTCAATTGATGAATTAGAAAAGTG
GTATGCTCCAGATCTAAACATATCGGCAAATTTCGAAGCAAATTCTCTCA
TGGAGAAATCGGACACAAACGGTGACAAAGTGCTAACATTCGACGAAATC
CTATCGAAACTAAATGAATGGAAAGACAGTCAAGCCACCAGTTATGGGAC
TCATATTCGAGATGAATTATAGAAACAAAAATCTTTATTTATTAATATAT
ATATATGCATTTGTAACACGATCAATAAATGCATCGGATCTCA
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protein sequence of SMED30014680-orf-1

>SMED30014680-orf-1 ID=SMED30014680-orf-1|Name=SMED30014680-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=292bp
MKMNLLSLKCSLVFLTLLYFVLSEEEILPNFSEQSITDNFGSYSVTKLRE
LLVKIDSDKNEIITKDELVEWIISLSLKDLDKRAKSDISFYDINKDNKIT
WIEMLRTTYGYNEESVEKILNSKDPIDKDNYQFIVDDRDRFKAADSDEDG
FLTQEEYKSLLNPSGSPKLLKVYMDKILSGHDKDNDSQLNEQEFKLYFYI
TEELTETQLLEFENIFLKYDFNKDRKLSIDELEKWYAPDLNISANFEANS
LMEKSDTNGDKVLTFDEILSKLNEWKDSQATSYGTHIRDEL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005788endoplasmic reticulum lumen
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 173..201
e-value: 23.0
score: 6.7
coord: 136..164
e-value: 15.0
score: 8.3
coord: 211..239
e-value: 2.7
score: 14.3
coord: 247..275
e-value: 15.0
score: 8.2
coord: 47..75
e-value: 1.1
score: 17.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 207..242
score: 9.994
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 243..278
score: 7.428
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 132..167
score: 10.078
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 169..204
score: 7.01
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 43..78
score: 9.604
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 79..114
score: 6.312
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 201..289
e-value: 1.6E-9
score: 39.5
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 126..200
e-value: 1.7E-8
score: 36.1
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 19..120
e-value: 1.5E-8
score: 36.7
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 39..290
NoneNo IPR availablePANTHERPTHR10827:SF52IP16409Pcoord: 39..290
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..23
score: 0.852
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 256..268
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 220..232
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 145..157
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 46..165
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 137..286