Low density lipoprotein-receptor, class A,domain-containing protein

Overview
NameLow density lipoprotein-receptor, class A,domain-containing protein
Smed IDSMED30014654
Length (bp)697
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014654) t-SNE clustered cells

Violin plots show distribution of expression levels for Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014654) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014654) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Low density lipoprotein-receptor, class A,domain-containing protein (SMED30014654) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014654

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30014654h1SMcG0015088 SmedASXL_002557SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30014654h1SMcG0015088 Contig42228newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014654h1SMcG0015088 Contig42228uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30014654h1SMcG0015088 dd_Smed_v4_2598_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014654h1SMcG0015088 dd_Smed_v4_2598_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30014654h1SMcG0015088 dd_Smed_v4_2598_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
non-ciliated neuronSMED30014654h1SMcG0015088 dd_Smed_v4_2598_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014654h1SMcG0015088 dd_Smed_v4_2598_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014654h1SMcG0015088 dd_Smed_v6_2598_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30014654h1SMcG0015088 OX_Smed_1.0.23435ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
GABAergic neuronSMED30014654h1SMcG0015088 dd_Smed_v6_2598_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30014654h1SMcG0015088 dd_Smed_v4_2598_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Celegans
Match: F58H1.7 (NMDA receptor auxiliary protein [Source:UniProtKB/TrEMBL;Acc:G5EEF9])

HSP 1 Score: 76.2554 bits (186), Expect = 5.513e-17
Identity = 40/138 (28.99%), Postives = 69/138 (50.00%), Query Frame = 2 
Query:  140 KTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYL-------------GQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIEL 514
            K    +   CP   P+ C + G+C+   ++CDG PDC D+YDE++S+CTA  RPP E    FL    S     +L             G G   ++A  L ++  A+ ++++MKL   + + +++++  +  G   EL
Sbjct:   26 KRGADAATPCPTWHPFACPS-GECVPIKYLCDGSPDCSDEYDENKSMCTAATRPPVEETQAFLKALMSAHGKDFLVKVFGPKAKAELSGMGGVDKVAVALSQTPTADLFASEMKLDDGETQHMLEVMEGILNGSTDEL 162
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Celegans
Match: F58H1.7 (NMDA receptor auxiliary protein [Source:UniProtKB/TrEMBL;Acc:G5EEF9])

HSP 1 Score: 75.8702 bits (185), Expect = 7.211e-17
Identity = 39/129 (30.23%), Postives = 67/129 (51.94%), Query Frame = 2 
Query:  167 CPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYL-------------GQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIEL 514
            CP   P+ C + G+C+   ++CDG PDC D+YDE++S+CTA  RPP E    FL    S     +L             G G   ++A  L ++  A+ ++++MKL   + + +++++  +  G   EL
Sbjct:   37 CPTWHPFACPS-GECVPIKYLCDGSPDCSDEYDENKSMCTAATRPPVEETQAFLKALMSAHGKDFLVKVFGPKAKAELSGMGGVDKVAVALSQTPTADLFASEMKLDDGETQHMLEVMEGILNGSTDEL 164
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Match: CG31221 (gene:FBgn0051221 transcript:FBtr0083762)

HSP 1 Score: 80.8777 bits (198), Expect = 2.768e-18
Identity = 46/156 (29.49%), Postives = 69/156 (44.23%), Query Frame = 2 
Query:  164 GCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQ-------------GTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
             C   +P+ C   G C+S  ++CDG PDC D YDED  LCTA  RPP E    FL    +   P YL +             G   ++A  L ES   E++   + L +   + L  +  AV+ G    L   G+      ++     ++ ++GFL
Sbjct:   63 ACHPYEPFKCPGDGNCISIQYLCDGAPDCSDGYDEDMRLCTAAKRPPVEETASFLQSLIASHGPNYLEKLFGSKARDALSPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSLGIKDSELGDVKFFLEKLVNTGFL 218
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Match: CG31221 (gene:FBgn0051221 transcript:FBtr0083763)

HSP 1 Score: 80.8777 bits (198), Expect = 2.768e-18
Identity = 46/156 (29.49%), Postives = 69/156 (44.23%), Query Frame = 2 
Query:  164 GCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQ-------------GTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
             C   +P+ C   G C+S  ++CDG PDC D YDED  LCTA  RPP E    FL    +   P YL +             G   ++A  L ES   E++   + L +   + L  +  AV+ G    L   G+      ++     ++ ++GFL
Sbjct:   63 ACHPYEPFKCPGDGNCISIQYLCDGAPDCSDGYDEDMRLCTAAKRPPVEETASFLQSLIASHGPNYLEKLFGSKARDALSPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSLGIKDSELGDVKFFLEKLVNTGFL 218
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Match: CG31221 (gene:FBgn0051221 transcript:FBtr0330030)

HSP 1 Score: 80.1073 bits (196), Expect = 8.158e-18
Identity = 46/156 (29.49%), Postives = 69/156 (44.23%), Query Frame = 2 
Query:  164 GCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQ-------------GTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
             C   +P+ C   G C+S  ++CDG PDC D YDED  LCTA  RPP E    FL    +   P YL +             G   ++A  L ES   E++   + L +   + L  +  AV+ G    L   G+      ++     ++ ++GFL
Sbjct:   63 ACHPYEPFKCPGDGNCISIQYLCDGAPDCSDGYDEDMRLCTAAKRPPVEETASFLQSLIASHGPNYLEKLFGSKARDALSPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSLGIKDSELGDVKFFLEKLVNTGFL 218
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Mouse
Match: Tmprss15 (transmembrane protease, serine 15 [Source:MGI Symbol;Acc:MGI:1197523])

HSP 1 Score: 48.9062 bits (115), Expect = 2.486e-6
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 2 
Query:  119 ASLAEESKT--AKSSGNGCPESQPW--PCKTPGQCLSFDFICDGEPDCPDKYDEDQSLC 283
            ASL   S T  A +SG+   E QP   PC     C++ D  CDGE +CPD  DED  LC
Sbjct:  193 ASLGNLSTTVAATTSGHVSIECQPGSRPCAHAWNCVATDLFCDGEVNCPDGSDEDTGLC 251
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Mouse
Match: Tmprss15 (transmembrane protease, serine 15 [Source:MGI Symbol;Acc:MGI:1197523])

HSP 1 Score: 47.3654 bits (111), Expect = 8.975e-6
Identity = 22/48 (45.83%), Postives = 27/48 (56.25%), Query Frame = 2 
Query:  146 AKSSGNGCPESQPW--PCKTPGQCLSFDFICDGEPDCPDKYDEDQSLC 283
            A +SG+   E QP   PC     C++ D  CDGE +CPD  DED  LC
Sbjct:  219 ATTSGHVSIECQPGSRPCAHAWNCVATDLFCDGEVNCPDGSDEDTGLC 266
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Match: sp|P85831|PROH4_APIME (Prohormone-4 OS=Apis mellifera OX=7460 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 6.004e-18
Identity = 48/161 (29.81%), Postives = 70/161 (43.48%), Query Frame = 2 
Query:  149 KSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQ-------------GTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            K SG+ C   +P+ C     C+S  ++CDG PDC D YDED  LCTA  RPP E    FL    +   P YL +             G    +A  L ES   E++   + L +   + L  +  AV+ G    L   G+      ++     ++  +GFL
Sbjct:   41 KRSGDACRPYEPFKCPGDDTCISIQYLCDGAPDCQDGYDEDSRLCTAAKRPPVEETASFLQSLLASHGPNYLEKLFGTKARDTLKPLGGVNTVAIALSESQTIEDFGAALHLLRTDLEHLRSVFMAVENGDLGMLKSIGIKDSELGDVKFFLEKLVKTGFL 201
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Match: sp|E2AX35|PROH4_CAMFO (IDLSRF-like peptide OS=Camponotus floridanus OX=104421 GN=EAG_01737 PE=1 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 3.853e-17
Identity = 46/157 (29.30%), Postives = 69/157 (43.95%), Query Frame = 2 
Query:  161 NGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQ-------------GTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            + C   +P+ C   G C+S  ++CDG PDC D YDED  LCTA  RPP E    FL    +   P YL +             G   ++A  L ES   E++   + L +   + L  +  AV+ G    L   G+      ++     ++  +GFL
Sbjct:   44 DACHPYEPFKCPGDGLCISIQYLCDGAPDCQDGYDEDSRLCTAAKRPPVEETATFLQSLLASHGPNYLEKLFGNKARDTLKPLGGVEKVAIALSESQTIEDFGAALHLMRSDLEHLRSVFMAVENGDLGMLKSIGIKDSELGDVKFFLEKLVKTGFL 200
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Match: sp|A0A0F7YYX3|CPROH_CONVC (Neuropeptide prohormone-4 OS=Conus victoriae OX=319920 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.792e-12
Identity = 40/145 (27.59%), Postives = 65/145 (44.83%), Query Frame = 2 
Query:  167 CPE----SQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGF 589
            CPE    + P+ C+    CL    +C+G  DC    DED +LC A  RP   ++ +FL     W++P          +A  L E+ +  +  + + LT Q  +++ +       G    L+  GMP RAW E+  L   + + G 
Sbjct:   47 CPEDCDIASPFKCEESPTCLRLFQVCNGRWDCEHGSDEDNALCAAVLRPLECMIWEFLEGQRDWILPNLFNDANTDLVAHALHEAYSMGDLQSYLNLTDQNIENIRNSTRGAIVGDPRPLMALGMPDRAWPEVMYLLKELYNLGL 191
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: G4LZA8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_121660 PE=4 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.080e-55
Identity = 89/166 (53.61%), Postives = 114/166 (68.67%), Query Frame = 2 
Query:   98 MLLYLAI-ASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            +LL L I  S      +A   GN CPESQPWPC+TP  CLSFDFICDGEPDCPD+YDED  +CTAK RP  E L  F+ +Y++WL+PK+LG GT  ++A  L+ES    +Y+   KL + Q K+L+ L+  VK G+++EL + GMP  AW+ELY LF RI  SGFL
Sbjct:   15 VLLSLIIYVSRQSTVSSAVERGNDCPESQPWPCRTPRICLSFDFICDGEPDCPDEYDEDPEMCTAKLRPAQEHLAGFIHRYQNWLVPKFLGYGTPEELAAKLVESKTVSDYARAAKLNETQLKNLIKLMEGVKSGKEMELFLLGMPLGAWSELYTLFHRIYISGFL 180
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A4S2L9Y8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008860 PE=4 SV=1)

HSP 1 Score: 186.037 bits (471), Expect = 1.226e-55
Identity = 92/171 (53.80%), Postives = 112/171 (65.50%), Query Frame = 2 
Query:   89 CTGMLLYLAIASLAEES---KTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            C   LL  A  +    +   K A + GN CPES+PWPC+TPG CLSF  ICDGEPDCP++YDEDQ +CTAK RP  E L  F+ KY  WLIP  LG+G    +AT L+ES N  +Y+    LTQ Q + L++LL  VK G+QI L M GMP  AW+ELY+LF RI  SGFL
Sbjct:    5 CVFFLLIFAFHTTVLRTGYVKAAANRGNDCPESKPWPCRTPGICLSFALICDGEPDCPEEYDEDQEMCTAKLRPAEEHLQGFINKYREWLIPDILGEGKPEDLATKLVESRNIVDYARAAHLTQAQVQRLIELLMGVKSGKQILLHMMGMPLGAWSELYVLFYRIYLSGFL 175
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: G7YUJ9 (Prohormone-4 OS=Clonorchis sinensis OX=79923 GN=CLF_111265 PE=4 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 2.766e-55
Identity = 92/179 (51.40%), Postives = 115/179 (64.25%), Query Frame = 2 
Query:   68 AVMLSSLCTGMLLYLAIASLAEES---KTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 595
            A+M+   C   LL  A  +    +   + A + GN CPES+PWPC+TPG CLSF  ICDGEPDCP++YDEDQ +CTAK RP  E L  F+ KY  WLIP  LG+G    +AT L+ES N  +Y+    LT  Q + L++LL  VK G+QI L M GMP  AW+ELY+LF RI  SGFL 
Sbjct:   13 AIMMFRSCVFFLLVFAFHTTVLRTGSVEAAANRGNDCPESKPWPCRTPGICLSFALICDGEPDCPEEYDEDQEMCTAKLRPAEEHLQGFINKYREWLIPDILGEGKPEDLATKLVESRNIVDYARAAHLTPAQVQRLVELLMGVKSGKQILLHMMGMPLGAWSELYVLFYRIYLSGFLN 191
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: W6UW14 (Prohormone-4 OS=Echinococcus granulosus OX=6210 GN=EGR_02423 PE=4 SV=1)

HSP 1 Score: 185.267 bits (469), Expect = 3.345e-55
Identity = 84/181 (46.41%), Postives = 118/181 (65.19%), Query Frame = 2 
Query:   71 VMLSSLCTGMLLYL-------AIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            V+   +  GM+ YL        I++ + E    K   N CPES PWPC+ PG CLSFDFICDGE DCPD YDED+ +CTAK RPP E L +F+ +Y+ WLIP +LG+GT  ++A  L+ES   E+Y+    LT++Q  +L  L+  V + +++ +++ GMP  AW+EL+ LF RI SSGF+
Sbjct:   34 VIQRVVVVGMIFYLLSKGICSTISARSNEKDAKKKIANECPESSPWPCREPGFCLSFDFICDGELDCPDGYDEDREMCTAKQRPPIEYLFRFIQRYQDWLIPTFLGKGTPLELAKKLVESPTIEDYARSAHLTEKQLVNLNSLIEGVVQRKEMHMVLLGMPLGAWSELFYLFNRIVSSGFV 214
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Match: A0A094ZWF8 (Uncharacterized protein OS=Schistosoma haematobium OX=6185 GN=MS3_05853 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 5.649e-55
Identity = 84/149 (56.38%), Postives = 107/149 (71.81%), Query Frame = 2 
Query:  146 AKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            A   GN CPESQPWPC+TP  CLSFDFICDGEPDCPD+YDED  +CTAK RP  E L  F+ +Y++WL+PK+LG GT  ++A  L+ES    +Y+   KL + Q K+L+ L+  VK G+++EL + GMP  AW+ELY LF RI  SGFL
Sbjct:   24 AVERGNDCPESQPWPCRTPRICLSFDFICDGEPDCPDEYDEDPEMCTAKLRPAQEHLAGFIHRYQNWLVPKFLGYGTPEELAAKLVESKTVSDYARAAKLNETQLKNLIKLMEGVKSGKEMELFLLGMPLGAWSELYTLFHRIYISGFL 172
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000021406.1 (SMESG000021406.1)

HSP 1 Score: 365.54 bits (937), Expect = 1.762e-130
Identity = 176/177 (99.44%), Postives = 177/177 (100.00%), Query Frame = 2 
Query:   65 MAVMLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 595
            M+VMLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK
Sbjct:    1 MSVMLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 177
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000021406.1 (SMESG000021406.1)

HSP 1 Score: 359.762 bits (922), Expect = 2.714e-128
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 2 
Query:   74 MLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 595
            MLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK
Sbjct:    1 MLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 174
[Close]
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000059509.1 (SMESG000059509.1)

HSP 1 Score: 219.164 bits (557), Expect = 2.111e-72
Identity = 97/146 (66.44%), Postives = 118/146 (80.82%), Query Frame = 2 
Query:  155 SGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            SGN CPES+PWPCKTPG CLSFDFICDGE DC D YDED++LCTAKNRPPF +L++F+ KY+ WLIP YLG GT  +++T+LIES N +EY   M +T ++ ++   LL +VKEGRQIELIM GMPPRAWNEL +LFGRI  SGF+
Sbjct:   49 SGNNCPESKPWPCKTPGVCLSFDFICDGEKDCDDNYDEDENLCTAKNRPPFIILNQFITKYKDWLIPNYLGSGTPEKVSTHLIESKNDQEYIKSMNMTPEEAENFKSLLESVKEGRQIELIMFGMPPRAWNELSILFGRIVKSGFM 194
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000059509.1 (SMESG000059509.1)

HSP 1 Score: 221.09 bits (562), Expect = 4.532e-72
Identity = 97/146 (66.44%), Postives = 118/146 (80.82%), Query Frame = 2 
Query:  155 SGNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFL 592
            SGN CPES+PWPCKTPG CLSFDFICDGE DC D YDED++LCTAKNRPPF +L++F+ KY+ WLIP YLG GT  +++T+LIES N +EY   M +T ++ ++   LL +VKEGRQIELIM GMPPRAWNEL +LFGRI  SGF+
Sbjct:  121 SGNNCPESKPWPCKTPGVCLSFDFICDGEKDCDDNYDEDENLCTAKNRPPFIILNQFITKYKDWLIPNYLGSGTPEKVSTHLIESKNDQEYIKSMNMTPEEAENFKSLLESVKEGRQIELIMFGMPPRAWNELSILFGRIVKSGFM 266
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Match: SMESG000037863.1 (SMESG000037863.1)

HSP 1 Score: 191.815 bits (486), Expect = 1.671e-61
Identity = 88/162 (54.32%), Postives = 115/162 (70.99%), Query Frame = 2 
Query:  122 SLAEESKTAKSS----GNGCPESQPWPCKTPGQCLSFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYLGQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIELIMEGMPPRAWNELYLLFGRIASSGFLK 595
            ++ E+ +  KS+    GN C E  PWPCKT G CLS DF+CDG+ DCPD YDED  +C AK+RPP+E+L +F+ KY+ WL+P YLG+     IAT LIES    EYS  M L ++Q  +L+ L+ AV+E R+IELI+ GMPPRAWNEL +LFGRI  SGF++
Sbjct:   47 NIEEKYRIRKSTYEFQGNQCLEKTPWPCKTSGYCLSLDFVCDGDFDCPDGYDEDVKMCIAKSRPPYEILKRFIEKYQDWLVPAYLGEADPGYIATQLIESPTINEYSKMMNLNKEQILNLISLMEAVREDREIELIIFGMPPRAWNELSILFGRITKSGFVQ 208
The following BLAST results are available for this feature:
BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
F58H1.75.513e-1728.99NMDA receptor auxiliary protein [Source:UniProtKB... [more]
F58H1.77.211e-1730.23NMDA receptor auxiliary protein [Source:UniProtKB... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CG312212.768e-1829.49gene:FBgn0051221 transcript:FBtr0083762[more]
CG312212.768e-1829.49gene:FBgn0051221 transcript:FBtr0083763[more]
CG312218.158e-1829.49gene:FBgn0051221 transcript:FBtr0330030[more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Tmprss152.486e-645.76transmembrane protease, serine 15 [Source:MGI Symb... [more]
Tmprss158.975e-645.83transmembrane protease, serine 15 [Source:MGI Symb... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|P85831|PROH4_APIME6.004e-1829.81Prohormone-4 OS=Apis mellifera OX=7460 PE=1 SV=1[more]
sp|E2AX35|PROH4_CAMFO3.853e-1729.30IDLSRF-like peptide OS=Camponotus floridanus OX=10... [more]
sp|A0A0F7YYX3|CPROH_CONVC3.792e-1227.59Neuropeptide prohormone-4 OS=Conus victoriae OX=31... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G4LZA81.080e-5553.61Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A4S2L9Y81.226e-5553.80Uncharacterized protein OS=Opisthorchis felineus O... [more]
G7YUJ92.766e-5551.40Prohormone-4 OS=Clonorchis sinensis OX=79923 GN=CL... [more]
W6UW143.345e-5546.41Prohormone-4 OS=Echinococcus granulosus OX=6210 GN... [more]
A0A094ZWF85.649e-5556.38Uncharacterized protein OS=Schistosoma haematobium... [more]
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Low density lipoprotein-receptor, class A,domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000021406.11.762e-13099.44SMESG000021406.1[more]
SMESG000021406.12.714e-128100.00SMESG000021406.1[more]
SMESG000059509.12.111e-7266.44SMESG000059509.1[more]
SMESG000059509.14.532e-7266.44SMESG000059509.1[more]
SMESG000037863.11.671e-6154.32SMESG000037863.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014654 ID=SMED30014654|Name=Low density lipoprotein-receptor, class A,domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=697bp
GACTATATCAATTCTGTTGCTTTCGTTTTATCAGTTTGATTAGTTTATTA
TTATAATTATTATTATGGCGGTTATGCTGAGCTCATTATGCACAGGAATG
CTGCTATACCTTGCAATTGCGTCACTTGCGGAAGAATCGAAAACTGCGAA
ATCAAGTGGAAATGGCTGTCCAGAAAGTCAACCCTGGCCTTGCAAAACCC
CTGGGCAATGTTTATCATTTGACTTTATTTGTGATGGTGAACCAGATTGT
CCAGATAAATACGACGAAGATCAAAGTCTTTGTACTGCTAAAAATCGGCC
CCCTTTCGAAGTGTTGGATAAGTTTTTGTTGAAATACGAGTCATGGCTTA
TACCGAAATATCTGGGCCAAGGAACTCATACTCAAATAGCAACATATTTG
ATTGAAAGTACTAATGCTGAAGAATACAGCAACAAAATGAAATTGACTCA
ACAGCAAAAAAAGGATCTGATGGATCTTTTGACTGCTGTGAAGGAGGGTC
GGCAAATCGAGCTCATAATGGAAGGAATGCCACCTAGAGCATGGAATGAA
TTGTACCTTCTCTTTGGTAGAATTGCTTCAAGTGGATTTCTGAAATAATT
TTCCCATCTGATTCTAGCATTGATGCTTCATCAAATAAATTTGTTGACTG
CATTTTGAAAATGACGTCTTTTTTTCTATTGAAATTGTTAATAAAAA
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protein sequence of SMED30014654-orf-1

>SMED30014654-orf-1 ID=SMED30014654-orf-1|Name=SMED30014654-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=178bp
MAVMLSSLCTGMLLYLAIASLAEESKTAKSSGNGCPESQPWPCKTPGQCL
SFDFICDGEPDCPDKYDEDQSLCTAKNRPPFEVLDKFLLKYESWLIPKYL
GQGTHTQIATYLIESTNAEEYSNKMKLTQQQKKDLMDLLTAVKEGRQIEL
IMEGMPPRAWNELYLLFGRIASSGFLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007218neuropeptide signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000028Category 2 cell
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000464GABAergic neuron
Vocabulary: INTERPRO
TermDefinition
IPR036055LDL_receptor-like_sf
IPR002172LDrepeatLR_classA_rpt
IPR023415LDLR_class-A_CS
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 118..138
NoneNo IPR availableGENE3DG3DSA:2.40.128.620coord: 33..94
e-value: 1.3E-8
score: 37.1
NoneNo IPR availablePANTHERPTHR20967UNCHARACTERIZEDcoord: 26..88
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.759
IPR002172Low-density lipoprotein (LDL) receptor class A repeatSMARTSM00192LDLa_2coord: 34..75
e-value: 1.3E-5
score: 34.7
IPR002172Low-density lipoprotein (LDL) receptor class A repeatPROSITEPS50068LDLRA_2coord: 34..74
score: 10.625
IPR002172Low-density lipoprotein (LDL) receptor class A repeatCDDcd00112LDLacoord: 35..69
e-value: 4.79917E-7
score: 42.579
IPR023415Low-density lipoprotein (LDL) receptor class A, conserved sitePROSITEPS01209LDLRA_1coord: 49..73
IPR036055LDL receptor-like superfamilySUPERFAMILYSSF57424LDL receptor-like modulecoord: 40..74