GCR149

Overview
NameGCR149
Smed IDSMED30014610
Length (bp)939
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR149 (SMED30014610) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR149 (SMED30014610) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014610

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30014610SMESG000009159.1 SmedASXL_007806SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30014610SMESG000009159.1 dd_Smed_v4_32664_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014610SMESG000009159.1 dd_Smed_v4_32664_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014610SMESG000009159.1 dd_Smed_v4_32664_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
testisSMED30014610SMESG000009159.1 GCR149smed_ncbi_20200123PMID:27163480
Saberi et al., 2016		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of GCR149 vs. TrEMBL
Match: A0A193KUU9 (GCR149 OS=Schmidtea mediterranea OX=79327 GN=gcr149 PE=2 SV=1)

HSP 1 Score: 422.935 bits (1086), Expect = 1.169e-161
Identity = 208/209 (99.52%), Postives = 208/209 (99.52%), Query Frame = 1 
Query:   52 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFYVDKK 678
            MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKP CVVLIAVIWNLISCIPHILAYGLFYVDKK
Sbjct:    1 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPLCVVLIAVIWNLISCIPHILAYGLFYVDKK 209

HSP 2 Score: 177.178 bits (448), Expect = 1.169e-161
Identity = 88/92 (95.65%), Postives = 90/92 (97.83%), Query Frame = 2 
Query:  662 FMLTKKFLCTDKLDRNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 937
            F + KKFLCTDKLD+NEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL
Sbjct:  204 FYVDKKFLCTDKLDQNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 295
[Close]
BLAST of GCR149 vs. TrEMBL
Match: A0A183VS48 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS3304 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.089e-13
Identity = 48/164 (29.27%), Postives = 89/164 (54.27%), Query Frame = 1 
Query:  193 LDSVIGNWSGYILPLFSIVGLFINIY-AIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFYVDKKI 681
            + S +G WSGYILP+   +GL IN + +I  F +IK       Y+  +A+  ++ + +  WL+ FPA G+PY+TNGK  +I +  S I+C+  +F +  +   S  +++ A++D   SI  P+  +       +C   +A + + I  +P      +++V+ K+
Sbjct:   44 ISSFLGAWSGYILPIACGLGLIINTFISITIFMKIKLMPRYMIYMAFVAIMSMLTNIIFGWLWLFPAKGVPYITNGKRYYIVYNVSPIACQSHRFLSSFTSTCSCNLLVCAALDRFFSIYFPTQFNKLPKYYAWCACGLAHLLSFIMMLPLFFTTDIYFVESKL 207
[Close]
BLAST of GCR149 vs. TrEMBL
Match: A0A4E0RPV6 (Putative myosin xvIII OS=Fasciola hepatica OX=6192 GN=D915_006225 PE=3 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.905e-11
Identity = 45/150 (30.00%), Postives = 77/150 (51.33%), Query Frame = 1 
Query:  193 LDSVIGNWSGYILPLFSIVGLFINIY--AIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAP----SNGSTKSWKKPFCVVLIAVIWNL 624
            + SV+G    YILP+   +GL  N+   A++ F+ ++    Q  Y   +A+ D     +  WL+ FPA GLPY TNG+ S+  F +S+  C+  +F    +   S  I++ A++D C S+  P    +   + +W     VVL++ +  L
Sbjct:  898 VSSVLGVLCAYILPVVCSLGLIGNVLTTALF-FHSLRPPTRQMIYFACLALSDAATQLLFGWLWLFPAKGLPYATNGRLSYFTFAQSSALCQLHRFAYSFTSTLSANILLLAAMDRCASVYWPLKIVNVPLSYAWYSILLVVLLSALMML 1046
[Close]
BLAST of GCR149 vs. TrEMBL
Match: A0A183MNZ0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS17765 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 7.792e-11
Identity = 44/142 (30.99%), Postives = 75/142 (52.82%), Query Frame = 1 
Query:  193 LDSVIGNWSGYILPLFSIVGLFIN-IYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVI 615
            +  V+G WS Y+LP+   +GL  + +  I  F  +K    Q  Y   ++  +I+ + +  WL+ FPA GLPY+TNG+  +  F  S I+C+  +F    S   +  +++ ++ID CLSI  P      S +  + + LI +I
Sbjct:   40 VTKVLGTWSAYLLPIICGLGLITHSLVVIIIFKCLKCIPRQLIYTGCLSCSNILTNILFAWLWLFPAKGLPYITNGQIYFFIFNTSRIACRLCRFTYSLSTTLTSNLILCSAIDRCLSIYFPMKLLHLSNRHAWYICLIVLI 181
[Close]
BLAST of GCR149 vs. TrEMBL
Match: A0A430QIB7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0006975 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 8.969e-11
Identity = 44/142 (30.99%), Postives = 74/142 (52.11%), Query Frame = 1 
Query:  193 LDSVIGNWSGYILPLFSIVGLFINIYAI-YKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVI 615
            +  V+G WS Y+LP+   +GL  +   I   F  +K    Q  Y   ++  +I+ + +  WL+ FPA GLPY+TNG+  +  F  S I+C+  +F    S   +  +++ ++ID CLSI  P      S +  + + LI +I
Sbjct:   40 VTKVLGIWSAYLLPIICGLGLITHCLVITIIFKCLKCTPRQLIYTGCLSCSNILTNILFAWLWLFPAKGLPYITNGQIYYFIFNTSRIACRLCRFTYSLSTTLTSNLILCSAIDRCLSIYFPVKLLHLSNRHAWYICLIVLI 181
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BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000009159.1 (SMESG000009159.1)

HSP 1 Score: 422.935 bits (1086), Expect = 6.493e-165
Identity = 208/209 (99.52%), Postives = 208/209 (99.52%), Query Frame = 1 
Query:   52 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFYVDKK 678
            MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKP CVVLIAVIWNLISCIPHILAYGLFYVDKK
Sbjct:    1 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPLCVVLIAVIWNLISCIPHILAYGLFYVDKK 209

HSP 2 Score: 177.178 bits (448), Expect = 6.493e-165
Identity = 88/92 (95.65%), Postives = 90/92 (97.83%), Query Frame = 2 
Query:  662 FMLTKKFLCTDKLDRNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 937
            F + KKFLCTDKLD+NEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL
Sbjct:  204 FYVDKKFLCTDKLDQNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 295
[Close]
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000009162.1 (SMESG000009162.1)

HSP 1 Score: 125.176 bits (313), Expect = 2.354e-33
Identity = 63/150 (42.00%), Postives = 96/150 (64.00%), Query Frame = 1 
Query:  217 SGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFY 666
            +GY  PL S +GLFINI  +Y+F    +   QK YL A+A+ D+  + + TW++ FPA GLP+++NG+  +I   +S I CKF+          SF I++F SID CLSI+ P +  T S+K    +V+++V+WN I+CIPH+L + L+Y
Sbjct:    2 TGYFNPLMSCLGLFINILIVYRFIINSRITRQKVYLSALAISDVSTNIIWTWMHLFPAFGLPFISNGQYYFILSSQSRIFCKFVYTTIFFWQDMSFLILLFCSIDRCLSILMPLDCRTWSYKSAISIVILSVVWNFINCIPHLLTFELYY 151
[Close]
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000009155.1 (SMESG000009155.1)

HSP 1 Score: 104.76 bits (260), Expect = 9.564e-26
Identity = 71/197 (36.04%), Postives = 105/197 (53.30%), Query Frame = 1 
Query:   52 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPH 642
            M N  LQI++    + ES N YS     A+ +L    N   Y K  Q D +    SG++ P+FSI+GL INI  I+ F++I        YL AIA  + I +   TW++ FP  GLP++T+ K  W    KS+  CKF+  +    +  SF I++  S+D CLSIM   +    S K    V++  V+WN++S +PH
Sbjct:    1 MPNSDLQIVIKYLASDESPNRYSTQFHCAVIRLDLPNNSLPYWKYCQYDRIFSIMSGFVNPIFSILGLIINIIVIFYFSKIGFT-RHTIYLSAIAGTETILNLFWTWIHLFPMWGLPFMTDSKYFWAISLKSDFFCKFIYSSIYFWNQMSFTILLACSVDRCLSIMIFFDSRHWSRKSAIKVIIGVVLWNVLSSLPH 196

HSP 2 Score: 30.8018 bits (68), Expect = 9.564e-26
Identity = 24/69 (34.78%), Postives = 40/69 (57.97%), Query Frame = 2 
Query:  716 LYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKL-EMKNAIQIFILS 919
            LY  Y   FHPFGVIYI L+ +  L+++ ++        L+R  N+  +  +  ++ E+K+ +QI ILS
Sbjct:  224 LYSFYQTLFHPFGVIYITLNVLVNLLLIKIVFSHSKQLKLVR--NSIGSGQSKRRVSEIKSCVQILILS 290
[Close]
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000022976.1 (SMESG000022976.1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.117e-12
Identity = 45/140 (32.14%), Postives = 67/140 (47.86%), Query Frame = 1 
Query:  202 VIGNWSGYILPLFSIVGLFINIYAIYKFN---QIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAV 612
            ++G +SGYI P   + G+  N    Y F      K    Q  YL A+A+ D        WL+ FPA GLPY++ G   +  F ++N +CK  ++    +   +  + +  SID CLSI  P   S K   K F   L+A+
Sbjct:   43 ILGVFSGYIFPFVGVFGIIFNFMIFYVFMIRYPPKLRTRQLIYLSALAIADNFSIITFGWLWIFPAKGLPYISKGLIYYFTFNENNPTCKAHRYIYSFASTLAVNLFLLTSIDRCLSIYKPLKYSKKG--KTFAWTLVAI 180
[Close]
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000052989.1 (SMESG000052989.1)

HSP 1 Score: 66.2402 bits (160), Expect = 5.597e-12
Identity = 52/175 (29.71%), Postives = 83/175 (47.43%), Query Frame = 1 
Query:  109 NSYSYLVSRALCKLTK-NLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFN-QIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSN----GSTKSWKKPFCVVLIAVI 615
            + +  L+S    KL + N N   +C  SQ   ++G WS ++ P+ SI G+F+NI  IY FN + +    Q  YL  +A FD        W++ F A GLPY ++G   +  + +  I CK  ++        +  I +  SID   +I  P         K+W+    VVL++ I
Sbjct:   18 SPWDLLISCEEVKLKQTNENNYLFCVFSQ---IMGFWSAFVFPVISIFGIFLNILIIYIFNFKFRDKTRQYIYLSFLAAFDSFNLICSGWMWIFFAKGLPYASSGVLYFFTYNQGVIPCKLHRYFTAAISCSAISIFLLTSIDRYFAIFWPLKCLNIPLKKAWQYSSMVVLLSFI 189
The following BLAST results are available for this feature:
BLAST of GCR149 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A193KUU91.169e-16199.52GCR149 OS=Schmidtea mediterranea OX=79327 GN=gcr14... [more]
A0A183VS484.089e-1329.27G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A4E0RPV63.905e-1130.00Putative myosin xvIII OS=Fasciola hepatica OX=6192... [more]
A0A183MNZ07.792e-1130.99G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A430QIB78.969e-1130.99G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of GCR149 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR149 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009159.16.493e-16599.52SMESG000009159.1[more]
SMESG000009162.12.354e-3342.00SMESG000009162.1[more]
SMESG000009155.19.564e-2636.04SMESG000009155.1[more]
SMESG000022976.11.117e-1232.14SMESG000022976.1[more]
SMESG000052989.15.597e-1229.71SMESG000052989.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014610 ID=SMED30014610|Name=GCR149|organism=Schmidtea mediterranea sexual|type=transcript|length=939bp
GATAAATACCAATGCAATGGTTGAAAATTTTATTTTTGAAAAAGTTTTCA
AATGGATAATGAAAGTCTACAAATTCTAGTATGGAATAAAGAAAATTCTG
AAAGCATCAATAGCTATTCGTACTTGGTGAGTAGGGCCCTGTGTAAATTA
ACGAAGAATTTAAACAAAGAATACTATTGCAAAGCAAGTCAACTTGATTC
GGTCATTGGAAATTGGAGTGGCTACATTTTGCCACTATTTAGTATAGTTG
GTCTATTCATAAACATTTACGCAATTTACAAATTTAACCAAATAAAACAA
AACATAGATCAAAAAACATATTTAATAGCAATCGCCGTGTTCGACATCAT
TGGCAGTTCAATGTTAACATGGCTTTATTACTTTCCGGCTGTCGGTTTAC
CATATGTTACTAACGGGAAATCCTCGTGGATATTTTTTCAAAAATCAAAT
ATTTCGTGCAAATTTATGCAATTCAATAACGACGGATCTCACGGGTTTTC
CTTTTTCATCATGATATTCGCCTCAATCGACAGCTGTCTCTCAATAATGG
CACCATCGAATGGCAGTACAAAATCTTGGAAAAAACCCTTTTGCGTTGTT
TTAATAGCGGTAATTTGGAATTTAATAAGCTGCATACCTCACATCTTGGC
GTATGGATTATTTTATGTTGACAAAAAAATTTTTATGTACGGATAAATTA
GACCGAAATGAGTATCTTTACAAGAACTACACGATATTTTTCCATCCTTT
TGGAGTTATTTATATTTGGTTAAGTTTCATGGCGTTGTTGGTCATTTTGA
TCGTGTTACAGAAACGATATGATATTTCATCATTAATCCGTCGTAAAAAC
GCTTATGAAAATTCGGTCAATGGAGAGAAGCTGGAAATGAAAAATGCGAT
TCAAATTTTTATTCTATCATCAATATCATTGGTGCTGGC
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protein sequence of SMED30014610-orf-1

>SMED30014610-orf-1 ID=SMED30014610-orf-1|Name=SMED30014610-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=215bp
MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDS
VIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDII
GSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFS
FFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILA
YGLFYVDKKIFMYG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 44..214
e-value: 8.5E-12
score: 46.6
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 57..201
e-value: 1.4808E-5
score: 42.62
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 41..202
NoneNo IPR availableTMHMMTMhelixcoord: 89..111
NoneNo IPR availableTMHMMTMhelixcoord: 180..202
NoneNo IPR availableTMHMMTMhelixcoord: 54..76
NoneNo IPR availableTMHMMTMhelixcoord: 148..167
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 67..214
score: 9.9