GCR149
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014610 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of GCR149 vs. TrEMBL
Match: A0A193KUU9 (GCR149 OS=Schmidtea mediterranea OX=79327 GN=gcr149 PE=2 SV=1) HSP 1 Score: 422.935 bits (1086), Expect = 1.169e-161 Identity = 208/209 (99.52%), Postives = 208/209 (99.52%), Query Frame = 1 Query: 52 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFYVDKK 678 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKP CVVLIAVIWNLISCIPHILAYGLFYVDKK Sbjct: 1 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPLCVVLIAVIWNLISCIPHILAYGLFYVDKK 209 HSP 2 Score: 177.178 bits (448), Expect = 1.169e-161 Identity = 88/92 (95.65%), Postives = 90/92 (97.83%), Query Frame = 2 Query: 662 FMLTKKFLCTDKLDRNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 937 F + KKFLCTDKLD+NEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL Sbjct: 204 FYVDKKFLCTDKLDQNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 295
BLAST of GCR149 vs. TrEMBL
Match: A0A183VS48 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS3304 PE=4 SV=1) HSP 1 Score: 79.7221 bits (195), Expect = 4.089e-13 Identity = 48/164 (29.27%), Postives = 89/164 (54.27%), Query Frame = 1 Query: 193 LDSVIGNWSGYILPLFSIVGLFINIY-AIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFYVDKKI 681 + S +G WSGYILP+ +GL IN + +I F +IK Y+ +A+ ++ + + WL+ FPA G+PY+TNGK +I + S I+C+ +F + + S +++ A++D SI P+ + +C +A + + I +P +++V+ K+ Sbjct: 44 ISSFLGAWSGYILPIACGLGLIINTFISITIFMKIKLMPRYMIYMAFVAIMSMLTNIIFGWLWLFPAKGVPYITNGKRYYIVYNVSPIACQSHRFLSSFTSTCSCNLLVCAALDRFFSIYFPTQFNKLPKYYAWCACGLAHLLSFIMMLPLFFTTDIYFVESKL 207
BLAST of GCR149 vs. TrEMBL
Match: A0A4E0RPV6 (Putative myosin xvIII OS=Fasciola hepatica OX=6192 GN=D915_006225 PE=3 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 3.905e-11 Identity = 45/150 (30.00%), Postives = 77/150 (51.33%), Query Frame = 1 Query: 193 LDSVIGNWSGYILPLFSIVGLFINIY--AIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAP----SNGSTKSWKKPFCVVLIAVIWNL 624 + SV+G YILP+ +GL N+ A++ F+ ++ Q Y +A+ D + WL+ FPA GLPY TNG+ S+ F +S+ C+ +F + S I++ A++D C S+ P + + +W VVL++ + L Sbjct: 898 VSSVLGVLCAYILPVVCSLGLIGNVLTTALF-FHSLRPPTRQMIYFACLALSDAATQLLFGWLWLFPAKGLPYATNGRLSYFTFAQSSALCQLHRFAYSFTSTLSANILLLAAMDRCASVYWPLKIVNVPLSYAWYSILLVVLLSALMML 1046
BLAST of GCR149 vs. TrEMBL
Match: A0A183MNZ0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS17765 PE=4 SV=1) HSP 1 Score: 73.1738 bits (178), Expect = 7.792e-11 Identity = 44/142 (30.99%), Postives = 75/142 (52.82%), Query Frame = 1 Query: 193 LDSVIGNWSGYILPLFSIVGLFIN-IYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVI 615 + V+G WS Y+LP+ +GL + + I F +K Q Y ++ +I+ + + WL+ FPA GLPY+TNG+ + F S I+C+ +F S + +++ ++ID CLSI P S + + + LI +I Sbjct: 40 VTKVLGTWSAYLLPIICGLGLITHSLVVIIIFKCLKCIPRQLIYTGCLSCSNILTNILFAWLWLFPAKGLPYITNGQIYFFIFNTSRIACRLCRFTYSLSTTLTSNLILCSAIDRCLSIYFPMKLLHLSNRHAWYICLIVLI 181
BLAST of GCR149 vs. TrEMBL
Match: A0A430QIB7 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0006975 PE=4 SV=1) HSP 1 Score: 73.1738 bits (178), Expect = 8.969e-11 Identity = 44/142 (30.99%), Postives = 74/142 (52.11%), Query Frame = 1 Query: 193 LDSVIGNWSGYILPLFSIVGLFINIYAI-YKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVI 615 + V+G WS Y+LP+ +GL + I F +K Q Y ++ +I+ + + WL+ FPA GLPY+TNG+ + F S I+C+ +F S + +++ ++ID CLSI P S + + + LI +I Sbjct: 40 VTKVLGIWSAYLLPIICGLGLITHCLVITIIFKCLKCTPRQLIYTGCLSCSNILTNILFAWLWLFPAKGLPYITNGQIYYFIFNTSRIACRLCRFTYSLSTTLTSNLILCSAIDRCLSIYFPVKLLHLSNRHAWYICLIVLI 181
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000009159.1 (SMESG000009159.1) HSP 1 Score: 422.935 bits (1086), Expect = 6.493e-165 Identity = 208/209 (99.52%), Postives = 208/209 (99.52%), Query Frame = 1 Query: 52 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFYVDKK 678 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKP CVVLIAVIWNLISCIPHILAYGLFYVDKK Sbjct: 1 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPLCVVLIAVIWNLISCIPHILAYGLFYVDKK 209 HSP 2 Score: 177.178 bits (448), Expect = 6.493e-165 Identity = 88/92 (95.65%), Postives = 90/92 (97.83%), Query Frame = 2 Query: 662 FMLTKKFLCTDKLDRNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 937 F + KKFLCTDKLD+NEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL Sbjct: 204 FYVDKKFLCTDKLDQNEYLYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKLEMKNAIQIFILSSISLVL 295
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000009162.1 (SMESG000009162.1) HSP 1 Score: 125.176 bits (313), Expect = 2.354e-33 Identity = 63/150 (42.00%), Postives = 96/150 (64.00%), Query Frame = 1 Query: 217 SGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPHILAYGLFY 666 +GY PL S +GLFINI +Y+F + QK YL A+A+ D+ + + TW++ FPA GLP+++NG+ +I +S I CKF+ SF I++F SID CLSI+ P + T S+K +V+++V+WN I+CIPH+L + L+Y Sbjct: 2 TGYFNPLMSCLGLFINILIVYRFIINSRITRQKVYLSALAISDVSTNIIWTWMHLFPAFGLPFISNGQYYFILSSQSRIFCKFVYTTIFFWQDMSFLILLFCSIDRCLSILMPLDCRTWSYKSAISIVILSVVWNFINCIPHLLTFELYY 151
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000009155.1 (SMESG000009155.1) HSP 1 Score: 104.76 bits (260), Expect = 9.564e-26 Identity = 71/197 (36.04%), Postives = 105/197 (53.30%), Query Frame = 1 Query: 52 MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFNQIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAVIWNLISCIPH 642 M N LQI++ + ES N YS A+ +L N Y K Q D + SG++ P+FSI+GL INI I+ F++I YL AIA + I + TW++ FP GLP++T+ K W KS+ CKF+ + + SF I++ S+D CLSIM + S K V++ V+WN++S +PH Sbjct: 1 MPNSDLQIVIKYLASDESPNRYSTQFHCAVIRLDLPNNSLPYWKYCQYDRIFSIMSGFVNPIFSILGLIINIIVIFYFSKIGFT-RHTIYLSAIAGTETILNLFWTWIHLFPMWGLPFMTDSKYFWAISLKSDFFCKFIYSSIYFWNQMSFTILLACSVDRCLSIMIFFDSRHWSRKSAIKVIIGVVLWNVLSSLPH 196 HSP 2 Score: 30.8018 bits (68), Expect = 9.564e-26 Identity = 24/69 (34.78%), Postives = 40/69 (57.97%), Query Frame = 2 Query: 716 LYKNYTIFFHPFGVIYIWLSFMALLVILIVLQKRYDISSLIRRKNAYENSVNGEKL-EMKNAIQIFILS 919 LY Y FHPFGVIYI L+ + L+++ ++ L+R N+ + + ++ E+K+ +QI ILS Sbjct: 224 LYSFYQTLFHPFGVIYITLNVLVNLLLIKIVFSHSKQLKLVR--NSIGSGQSKRRVSEIKSCVQILILS 290
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000022976.1 (SMESG000022976.1) HSP 1 Score: 68.1662 bits (165), Expect = 1.117e-12 Identity = 45/140 (32.14%), Postives = 67/140 (47.86%), Query Frame = 1 Query: 202 VIGNWSGYILPLFSIVGLFINIYAIYKFN---QIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSNGSTKSWKKPFCVVLIAV 612 ++G +SGYI P + G+ N Y F K Q YL A+A+ D WL+ FPA GLPY++ G + F ++N +CK ++ + + + + SID CLSI P S K K F L+A+ Sbjct: 43 ILGVFSGYIFPFVGVFGIIFNFMIFYVFMIRYPPKLRTRQLIYLSALAIADNFSIITFGWLWIFPAKGLPYISKGLIYYFTFNENNPTCKAHRYIYSFASTLAVNLFLLTSIDRCLSIYKPLKYSKKG--KTFAWTLVAI 180
BLAST of GCR149 vs. Planmine SMEST
Match: SMESG000052989.1 (SMESG000052989.1) HSP 1 Score: 66.2402 bits (160), Expect = 5.597e-12 Identity = 52/175 (29.71%), Postives = 83/175 (47.43%), Query Frame = 1 Query: 109 NSYSYLVSRALCKLTK-NLNKEYYCKASQLDSVIGNWSGYILPLFSIVGLFINIYAIYKFN-QIKQNIDQKTYLIAIAVFDIIGSSMLTWLYYFPAVGLPYVTNGKSSWIFFQKSNISCKFMQFNNDGSHGFSFFIMIFASIDSCLSIMAPSN----GSTKSWKKPFCVVLIAVI 615 + + L+S KL + N N +C SQ ++G WS ++ P+ SI G+F+NI IY FN + + Q YL +A FD W++ F A GLPY ++G + + + I CK ++ + I + SID +I P K+W+ VVL++ I Sbjct: 18 SPWDLLISCEEVKLKQTNENNYLFCVFSQ---IMGFWSAFVFPVISIFGIFLNILIIYIFNFKFRDKTRQYIYLSFLAAFDSFNLICSGWMWIFFAKGLPYASSGVLYFFTYNQGVIPCKLHRYFTAAISCSAISIFLLTSIDRYFAIFWPLKCLNIPLKKAWQYSSMVVLLSFI 189 The following BLAST results are available for this feature:
BLAST of GCR149 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of GCR149 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of GCR149 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of GCR149 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of GCR149 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of GCR149 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of GCR149 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of GCR149 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014610 ID=SMED30014610|Name=GCR149|organism=Schmidtea mediterranea sexual|type=transcript|length=939bpback to top protein sequence of SMED30014610-orf-1 >SMED30014610-orf-1 ID=SMED30014610-orf-1|Name=SMED30014610-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=215bp MDNESLQILVWNKENSESINSYSYLVSRALCKLTKNLNKEYYCKASQLDSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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