Peptidase M60 domain-containing protein

Overview
NamePeptidase M60 domain-containing protein
Smed IDSMED30014608
Length (bp)3429
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Peptidase M60 domain-containing protein (SMED30014608) t-SNE clustered cells

Violin plots show distribution of expression levels for Peptidase M60 domain-containing protein (SMED30014608) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Peptidase M60 domain-containing protein (SMED30014608) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Peptidase M60 domain-containing protein (SMED30014608) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30014608

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30014608h1SMcG0018637 Contig6449uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014608h1SMcG0018637 Contig6449newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
parapharyngeal regionSMED30014608h1SMcG0018637 SmedASXL_017806SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30014608h1SMcG0018637 dd_Smed_v4_11830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014608h1SMcG0018637 dd_Smed_v4_11830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014608h1SMcG0018637 dd_Smed_v4_11830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014608h1SMcG0018637 dd_Smed_v4_11830_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2 (TRPM8 channel associated factor 2 [Source:HGNC Symbol;Acc:HGNC:26878])

HSP 1 Score: 133.65 bits (335), Expect = 4.598e-31
Identity = 100/370 (27.03%), Postives = 165/370 (44.59%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYL  G+    +L + + S    + +  H  D+ +  +  + PV      +   E ++ S  +GGLL +     +    V +   G +  P +    +   +    +Q + APW  LA D+ +  V    ++ L  P  VL L+D M++    L       +R E I  D+QI AG+MH+GYPI   ++ V       K + +  +M   G WG +HE+GHN Q   + F   T + T N++++   E++ G      +E   S      R   +L +G    +W    + AL  Y QL+  FGWE + Q+F +Y   +   K   +T ++  W+   S   K NL P ++ W WPI       L   P
Sbjct:  444 GLYLLEGQNAEVSLSEAAASAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDRTE-RSVSCLWGGLLYVIVPKGSQLGPVPVTIRGAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKDPEPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESV-------KEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEA-LSPPERERRIKAHLGKGAPLCDWNV--WTALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKD--NTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 800          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2 (TRPM8 channel associated factor 2 [Source:HGNC Symbol;Acc:HGNC:26878])

HSP 1 Score: 133.65 bits (335), Expect = 4.598e-31
Identity = 100/370 (27.03%), Postives = 165/370 (44.59%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYL  G+    +L + + S    + +  H  D+ +  +  + PV      +   E ++ S  +GGLL +     +    V +   G +  P +    +   +    +Q + APW  LA D+ +  V    ++ L  P  VL L+D M++    L       +R E I  D+QI AG+MH+GYPI   ++ V       K + +  +M   G WG +HE+GHN Q   + F   T + T N++++   E++ G      +E   S      R   +L +G    +W    + AL  Y QL+  FGWE + Q+F +Y   +   K   +T ++  W+   S   K NL P ++ W WPI       L   P
Sbjct:  444 GLYLLEGQNAEVSLSEAAASAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDRTE-RSVSCLWGGLLYVIVPKGSQLGPVPVTIRGAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKDPEPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESV-------KEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEA-LSPPERERRIKAHLGKGAPLCDWNV--WTALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKD--NTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 800          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2 (TRPM8 channel associated factor 2 [Source:HGNC Symbol;Acc:HGNC:26878])

HSP 1 Score: 133.65 bits (335), Expect = 5.358e-31
Identity = 100/370 (27.03%), Postives = 165/370 (44.59%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYL  G+    +L + + S    + +  H  D+ +  +  + PV      +   E ++ S  +GGLL +     +    V +   G +  P +    +   +    +Q + APW  LA D+ +  V    ++ L  P  VL L+D M++    L       +R E I  D+QI AG+MH+GYPI   ++ V       K + +  +M   G WG +HE+GHN Q   + F   T + T N++++   E++ G      +E   S      R   +L +G    +W    + AL  Y QL+  FGWE + Q+F +Y   +   K   +T ++  W+   S   K NL P ++ W WPI       L   P
Sbjct:  548 GLYLLEGQNAEVSLSEAAASAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDRTE-RSVSCLWGGLLYVIVPKGSQLGPVPVTIRGAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKDPEPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESV-------KEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEA-LSPPERERRIKAHLGKGAPLCDWNV--WTALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKD--NTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 904          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF2C (TRPM8 channel associated factor 2C [Source:HGNC Symbol;Acc:HGNC:53641])

HSP 1 Score: 132.494 bits (332), Expect = 7.398e-31
Identity = 100/372 (26.88%), Postives = 166/372 (44.62%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYF 3074
            GLYL  G+    +L + + S    + +  H  D+ +  +  + PV      +   E ++ S  +GGLL +     +    V +   G +  P +    +   +    +Q + APW  LA D+ +  V    ++ L  P  VL L+D M++    L       +R E I  D+QI AG+MH+GYPI   ++ V       K + +  +M   G WG +HE+GHN Q   + F   T + T N++++   E++ G      +E   S      R   +L +G    +W    + AL  Y QL+  FGWE + Q+F +Y   +   K   +T ++  W+   S   K NL P ++ W WPI       L   P +
Sbjct:  341 GLYLLEGQNAEVSLSEAAASAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDRTE-RSVSCLWGGLLYVIVPKGSQLGPVPVTIRGAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKDPEPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESV-------KEIINEMDMRSRGVWGPIHELGHNQQWHGWEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEA-LSPPERERRIKAHLGKGAPLCDWNV--WTALETYLQLQQAFGWEPFTQLFAEYQTLSHLPKD--NTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLPEW 699          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Match: TCAF1 (TRPM8 channel associated factor 1 [Source:HGNC Symbol;Acc:HGNC:22201])

HSP 1 Score: 122.479 bits (306), Expect = 1.403e-27
Identity = 93/371 (25.07%), Postives = 161/371 (43.40%), Query Frame = 3
Query: 1974 GLYLPPGKI-GTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEG-WASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLY+P  +I   +L + + S    + +  H  D+ +  + ++ P+      L     ++ +  +GGLL I    ++    V +   G +  P +   ++   +    +Q +  PW  LA D+ +  V    +R L  P  +L L+D +++    L       +  + I  D+QI  G+MHAGYPI   ++ V         L +   +  +G WG +HE+G N Q   + F   T + T N++ +   E++ G      N   W   R    R   YL++G     W    + AL  Y QL+  FGWE + ++F +Y  +        D  K+  W+ + S   + NL P ++ W WPI       L   P
Sbjct:  548 GLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLD-KPTKSITCLWGGLLYIIVPQNSKLGSVPVTVKGAVHAPYYKLGETTLEEWKRRIQENPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQE-------LINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVRE--KRVRIYLSKGPNVKNWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTNLPTENVD--KMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSLAYLP 904          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Match: cam-1 (Tyrosine-protein kinase receptor cam-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EGK5])

HSP 1 Score: 58.5362 bits (140), Expect = 3.132e-8
Identity = 33/82 (40.24%), Postives = 43/82 (52.44%), Query Frame = 3
Query:  369 NWCKDTAEGQEYFGSLNLTESGKDCKLW--SLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            +WC     G +Y G++  T SGK C  W  S    F  + FPE  L N++NYCR+P G    PWCY      + + C VPQC
Sbjct:  326 HWCY-VNSGTQYEGTVAQTSSGKQCAPWIDSTSRDFNVHRFPE--LMNSKNYCRNPGGKKSRPWCYSKPMG-QEEYCDVPQC 403          

HSP 2 Score: 55.8398 bits (133), Expect = 2.172e-7
Identity = 32/101 (31.68%), Postives = 50/101 (49.50%), Query Frame = 3
Query:   78 LSATYFTEIKKCKIT-----SHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKD 365
            L ++   E+ +  +T     + G++Y G V +T SGK+C  W     R  N+    F +L  ++  N+CRNP      PWC +     Q E+CD+P C  D
Sbjct:  311 LQSSPVAEVNRGHLTHWCYVNSGTQYEGTVAQTSSGKQCAPWIDSTSRDFNVHR--FPEL--MNSKNYCRNPGGKKSRPWCYSKPMG-QEEYCDVPQCPSD 406          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Match: cam-1 (Tyrosine-protein kinase receptor cam-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EGK5])

HSP 1 Score: 58.151 bits (139), Expect = 3.356e-8
Identity = 33/82 (40.24%), Postives = 43/82 (52.44%), Query Frame = 3
Query:  369 NWCKDTAEGQEYFGSLNLTESGKDCKLW--SLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            +WC     G +Y G++  T SGK C  W  S    F  + FPE  L N++NYCR+P G    PWCY      + + C VPQC
Sbjct:  352 HWCY-VNSGTQYEGTVAQTSSGKQCAPWIDSTSRDFNVHRFPE--LMNSKNYCRNPGGKKSRPWCYSKPMG-QEEYCDVPQC 429          

HSP 2 Score: 55.8398 bits (133), Expect = 2.155e-7
Identity = 32/101 (31.68%), Postives = 50/101 (49.50%), Query Frame = 3
Query:   78 LSATYFTEIKKCKIT-----SHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKD 365
            L ++   E+ +  +T     + G++Y G V +T SGK+C  W     R  N+    F +L  ++  N+CRNP      PWC +     Q E+CD+P C  D
Sbjct:  337 LQSSPVAEVNRGHLTHWCYVNSGTQYEGTVAQTSSGKQCAPWIDSTSRDFNVHR--FPEL--MNSKNYCRNPGGKKSRPWCYSKPMG-QEEYCDVPQCPSD 432          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Match: cam-1 (Tyrosine-protein kinase receptor cam-1 [Source:UniProtKB/Swiss-Prot;Acc:G5EGK5])

HSP 1 Score: 58.151 bits (139), Expect = 3.519e-8
Identity = 33/82 (40.24%), Postives = 43/82 (52.44%), Query Frame = 3
Query:  369 NWCKDTAEGQEYFGSLNLTESGKDCKLW--SLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            +WC     G +Y G++  T SGK C  W  S    F  + FPE  L N++NYCR+P G    PWCY      + + C VPQC
Sbjct:  193 HWCY-VNSGTQYEGTVAQTSSGKQCAPWIDSTSRDFNVHRFPE--LMNSKNYCRNPGGKKSRPWCYSKPMG-QEEYCDVPQC 270          

HSP 2 Score: 55.4546 bits (132), Expect = 2.291e-7
Identity = 32/101 (31.68%), Postives = 50/101 (49.50%), Query Frame = 3
Query:   78 LSATYFTEIKKCKIT-----SHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKD 365
            L ++   E+ +  +T     + G++Y G V +T SGK+C  W     R  N+    F +L  ++  N+CRNP      PWC +     Q E+CD+P C  D
Sbjct:  178 LQSSPVAEVNRGHLTHWCYVNSGTQYEGTVAQTSSGKQCAPWIDSTSRDFNVHR--FPEL--MNSKNYCRNPGGKKSRPWCYSKPMG-QEEYCDVPQCPSD 273          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Match: Nrk (gene:FBgn0020391 transcript:FBtr0087728)

HSP 1 Score: 61.6178 bits (148), Expect = 3.336e-9
Identity = 28/76 (36.84%), Postives = 43/76 (56.58%), Query Frame = 3
Query:  393 GQEYFGSLNLTESGKDCKLWSLQNKFPANGF-PEM---SLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            G+ Y G++N+++SG  C+ W  Q  +P   F P +    L   ENYCR+  G    PWCY  D +++W +C +P C
Sbjct:  224 GRFYMGTMNVSKSGIPCQRWDTQ--YPHKHFQPPLVFHQLLEGENYCRNAGGEEPHPWCYTVDESVRWQHCDIPMC 297          

HSP 2 Score: 56.6102 bits (135), Expect = 1.283e-7
Identity = 31/104 (29.81%), Postives = 52/104 (50.00%), Query Frame = 3
Query:   72 PNLSATYFTEIKKCKIT-----SHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF----LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFC 356
            PN S    TE+K+ +++      +G  Y+G +  + SG  C +W  +         KHF    +  + +   N+CRN       PWC T +++++W+HCDIP C
Sbjct:  200 PNCSYIGLTELKESEVSYDCRNGNGRFYMGTMNVSKSGIPCQRWDTQY------PHKHFQPPLVFHQLLEGENYCRNAGGEEPHPWCYTVDESVRWQHCDIPMC 297          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Match: Ror (gene:FBgn0010407 transcript:FBtr0079978)

HSP 1 Score: 53.5286 bits (127), Expect = 1.083e-6
Identity = 28/74 (37.84%), Postives = 40/74 (54.05%), Query Frame = 3
Query:  390 EGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIK-WDNCYVPQC 608
            +G  Y G  N++ SGK C  WS   K   + FPE+     +NYCR+P      PWC+VD S  +  + C +P+C
Sbjct:  241 DGSTYRGVANVSASGKPCLRWSWLMK-EISDFPELI---GQNYCRNPGSVENSPWCFVDSSRERIIELCDIPKC 310          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.3 (si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-13])

HSP 1 Score: 126.331 bits (316), Expect = 5.499e-29
Identity = 102/386 (26.42%), Postives = 172/386 (44.56%), Query Frame = 3
Query: 1926 VQMALNLKFKDETSQLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            V++  N    +E    GLYL PG K    + +        + L     +I   +   + PV      L   ++Q  +  +GGL+      ++  D V++V    +Q P F   ++        +++  APWA L  ++ V  +  + +RNL +P +V +L+D +++    L     +  R+E    D+QI AG+MH+GYPI  +       S  +  L +V        WG LH++GHN Q   + F   T + T N++++   E + G +  ++     + +S   R   Y + GK F+ W    + AL  Y QL+  FGW+ + +VF  Y+          +  K+  +    S     NL P +  W WPI  NT QEL   P
Sbjct:  529 VRITANTADSEEWISTGLYLCPGMKTYITIPQEIIGTNWQVQLGCQTNNIGGSNVLKRAPVVHERFPLDSEKVQVCNL-WGGLIYFVAPPESKVDGVEIVVQTAVQAPYFKSGETSVADWVGKIRQRPAPWAELEFENLVMTLPSDFIRNLERPDQVAKLWDAIMRSVSDLAATPAKFPRKERFVGDVQISAGFMHSGYPIMML-------SSSASELLNVKEAHKHDFWGPLHKLGHNQQRKVWEFPPHTTECTCNLWSVYVHEKVLGVNK-AKAHPCMTLQSRQERIKEYFSGGKDFENWNT--WTALETYMQLQEKFGWDAFKKVFSLYHDMKDVPND--NAGKMNLYAKTFSKVVNHNLSPFFKVWGWPIQPNTEQELSHLP 901          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:162193 (zgc:162193 [Source:NCBI gene;Acc:323315])

HSP 1 Score: 124.79 bits (312), Expect = 2.067e-28
Identity = 106/387 (27.39%), Postives = 174/387 (44.96%), Query Frame = 3
Query: 1926 VQMALNLKFKDETSQLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTG-KSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            V++  N    +E    GLYL PG K   A+      +   + L     +I   +   + PV      L    +Q ++  +GGL+ +        D V++V    +Q P F   ++      + ++++ APWA L  ++ +       +RNL +P +V +L+D +++    L     +  R+E    D+QI  G+MHAGYPI  M+   + P      L +V      G WG +HE+GHN Q   + F   T + T N+++L   E + G KS+ +      +DR    R   Y + GK  + W    ++AL  Y QL+  FGW+ + +VF  Y+          +  K+  +    S    +NL P +  W WPI  NT Q L   P
Sbjct:  529 VRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNL-WGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPI--MMHSGSAPG-----LVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQ--ARTKMYFDGGKDLNSWCM--WMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPND--NAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLP 901          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:162193 (zgc:162193 [Source:NCBI gene;Acc:323315])

HSP 1 Score: 124.79 bits (312), Expect = 2.067e-28
Identity = 106/387 (27.39%), Postives = 174/387 (44.96%), Query Frame = 3
Query: 1926 VQMALNLKFKDETSQLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTG-KSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            V++  N    +E    GLYL PG K   A+      +   + L     +I   +   + PV      L    +Q ++  +GGL+ +        D V++V    +Q P F   ++      + ++++ APWA L  ++ +       +RNL +P +V +L+D +++    L     +  R+E    D+QI  G+MHAGYPI  M+   + P      L +V      G WG +HE+GHN Q   + F   T + T N+++L   E + G KS+ +      +DR    R   Y + GK  + W    ++AL  Y QL+  FGW+ + +VF  Y+          +  K+  +    S    +NL P +  W WPI  NT Q L   P
Sbjct:  529 VRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNL-WGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPI--MMHSGSAPG-----LVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQ--ARTKMYFDGGKDLNSWCM--WMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPND--NAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLP 901          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: zgc:162193 (zgc:162193 [Source:NCBI gene;Acc:323315])

HSP 1 Score: 124.79 bits (312), Expect = 2.067e-28
Identity = 106/387 (27.39%), Postives = 174/387 (44.96%), Query Frame = 3
Query: 1926 VQMALNLKFKDETSQLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTG-KSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            V++  N    +E    GLYL PG K   A+      +   + L     +I   +   + PV      L    +Q ++  +GGL+ +        D V++V    +Q P F   ++      + ++++ APWA L  ++ +       +RNL +P +V +L+D +++    L     +  R+E    D+QI  G+MHAGYPI  M+   + P      L +V      G WG +HE+GHN Q   + F   T + T N+++L   E + G KS+ +      +DR    R   Y + GK  + W    ++AL  Y QL+  FGW+ + +VF  Y+          +  K+  +    S    +NL P +  W WPI  NT Q L   P
Sbjct:  529 VRITANTGGCEEWISTGLYLSPGMKTYIAVPPEIVGKNWQVQLGCQTDNIGGSNTLKRAPVVHARFPLDSEMVQVWNL-WGGLIYLIAPSQTKVDGVEIVVQTAVQAPYFKSGETSVADWVSHIRQAPAPWAELEFENLIMTFDSAFIRNLDRPDEVAKLWDTIMRTITDLAARPPKLPRKERFVADVQISYGFMHAGYPI--MMHSGSAPG-----LVNVEEAYKCGLWGAIHELGHNQQRGVWEFPPHTTECTCNLWSLYVHEQVFGIKSANAHPAITPADRQ--ARTKMYFDGGKDLNSWCM--WMALETYMQLQEKFGWDAFKKVFSLYHDMTGVPND--NAGKMNLYAQTFSKVVNLNLSPFFKAWGWPIQPNTEQNLSHLP 901          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Match: si:ch211-210b2.4 (si:ch211-210b2.4 [Source:ZFIN;Acc:ZDB-GENE-131122-12])

HSP 1 Score: 122.094 bits (305), Expect = 1.322e-27
Identity = 104/388 (26.80%), Postives = 168/388 (43.30%), Query Frame = 3
Query: 1926 VQMALNLKFKDETSQLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTG--KSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            V++  N    +E    GLYL PG K    +      +   + L     DI +     + PV      L   ++Q  +  +GGL+       +  D V++V    +Q P F   ++        +++  APWA L  ++ V  +  + +RNL +P +V EL+D +++    L     +  R+E    D+QI AG+MH+GYPI  +       S  +  L +V        WG LHE+GHN Q   + F   T + T N++++   E + G  ++         S ++ I +   Y + GK    W      AL  Y QL+  FGW+ + +VF  Y+          +  K+  +    S     NL P +  W WPI  NT QEL   P
Sbjct:  529 VRITANTAGCEEWISTGLYLSPGMKTYITIPPEIIEKKWQVQLGCQTDDIGRLKILKRAPVVHEQFPLDSEKVQVCNL-WGGLIYFVAPPKSKVDGVEIVVQTAVQAPYFKSGETSVADWVGKIRQRPAPWAELEFENLVMTLPSDLIRNLERPDEVAELWDAIMRSVSDLAATPAKFPRKERFVGDVQISAGFMHSGYPIMML-------SSSASQLLNVKEAHKHDFWGPLHELGHNQQRKVWEFPPHTTECTCNLWSVYVHEKVLGVNRAKAHPCMTLESRQACIKK---YFSGGKDLKNWTKA--TALETYMQLQEKFGWDAFKKVFSLYHDMKDVPND--NAGKMNLYAKTFSKVVNHNLSPFFKTWGWPIQPNTEQELSHLP 901          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045101.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 150.214 bits (378), Expect = 3.145e-36
Identity = 108/381 (28.35%), Postives = 173/381 (45.41%), Query Frame = 3
Query: 1956 DETSQLGLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEG-KFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPD 3083
            D     GLYL P K    +F  S  Q  + + +  H  D+  KDE+Y+ P+  R I +   ++Q   + +GGLL I     ++   + +   G    P + +  +  +     L+    PWA L  ++ +  V  + +R+L  P ++L  +D M+     L  I     R E I  D+QI AG+MHAGYP+   +  V       K+L ++  M  EG WG +HE+GHN Q   + F   T + T N++++   E++ G      N       S       YL  G K + W    + AL  Y QL+  FGWE + Q+F+ Y + +       +  K+  W    S   + NL P ++ W WPI+  T  +L   P +  D
Sbjct:  527 DAWRSTGLYLSPKKTAVLVFPASAVQQGLQVQVGCHSDDLGSKDEFYRAPIVVRKISVHREKVQVSCY-WGGLLYIIVKERSNLGTIPVQVYGAEPAPTYIKGKTSLDSWLQSLRDLPPPWAELITENIILTVPSDAIRSLNDPEELLGFWDRMMVAITELAAIPTTFSRPERIVADVQISAGWMHAGYPVMVHLASV-------KYLTNLSCMQKEGLWGPIHELGHNQQQKVWEFPPHTTEATCNLWSVFVNETVVGVPRDKANSA-LEPASRAACIEAYLKTGPKLENWNV--WTALETYLQLQEGFGWEPFKQLFKDYQSMSGISND--NKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKD 894          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000002244.1 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:100489234])

HSP 1 Score: 143.665 bits (361), Expect = 3.463e-34
Identity = 109/391 (27.88%), Postives = 182/391 (46.55%), Query Frame = 3
Query: 1956 DETSQLGLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNK 3113
            D     GLYLPP K     F  S  Q    + +  H  D   K+   + PV  R I +   ++   SF +GGLL I    +++   V +   G    P++ +  +  +   + +++  APWA L  ++ +  V  + VR+L  P  +L L+D ++     L     +  R E I  D+QI AG+MHAGYPI   ++        +K L D+ +M  EG WG +HE+GHN Q + + F   T + T N++ +   E++ G     +  G        +R  +YL  G   ++W    + AL  Y QL+   GW+ + +++R  +   +  +   +T K+  W    S  A+ NL P ++ W WPID +T  +L   P +  D     + + K
Sbjct:  505 DAWRSTGLYLPPRKAVVIEFPASAVQQGFQVQVGCHSDDPSAKETLNRAPVVVRRIHVDRQKVPVSSF-WGGLLYIIVRSNSNFGTVPVRVYGAEPAPIYIKGKTSLDSWLHSIRQLPAPWAELVTENIILTVPSDAVRSLKNPEALLSLWDRIMAAIADLAAKPKKFPRPERIVADVQISAGWMHAGYPIMCDLE-------SAKELTDLHHMEKEGIWGPIHELGHNQQKTQWEFPPHTTEATCNLWAVYVHETVLGIPR-DRAHGNLQPNVRTSRIKSYLQNGANLEQWDG--WTALETYLQLQEGLGWDPFRRLYRDPSISGMSNE---NTFKMNLWAEKFSRAAQTNLVPFFEAWGWPIDQSTRSKLSALPVWEKDPMKPYVTAKK 881          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045059.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 135.576 bits (340), Expect = 1.169e-31
Identity = 104/385 (27.01%), Postives = 176/385 (45.71%), Query Frame = 3
Query: 1956 DETSQLGLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDK--VDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWAS-----DRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPD 3083
            D     GLYL P K     F  S  Q  + + +     D+   ++Y + PV  R   +   ++    F +GGLL I    +++   + +   G    PV+ +  ++ +     ++   APWA L  ++ +  V  + +R+L+ P  +L L+D ++     L  I     R E    D+QI AG+MHAGYPI   ++        +K L D+  M   G WG +HE+GHN Q  ++ F   T + T N++++   E++ G     +N+   S        YI  +V   N    ++W  + + AL  Y QL+  FGWE + Q+F+ Y + +       +  K+  W    S   + NL P ++ W WPI+  T  +L   P +  D
Sbjct:  527 DAWRSTGLYLAPRKTAVLEFPASVVQQGLKVQVGCQADDLSSAEKYCRAPVVVRKCHVDRQKVSVSCF-WGGLLYIIVKANSNLGIIPVKVYGAEPAPVYIKGKTNLDSWLQSVRYLPAPWAELITENIILTVPSDAIRSLSDPEALLSLWDKIMVSITELAAIPKTFPRPERFVADVQISAGWMHAGYPIMCHLE-------SAKELTDLNVMQTVGIWGPIHELGHNQQKINWEFPPHTTEATCNLWSVYVHETVLGI---PRNKAHGSLQPETRACYIQEYVK--NGSNLEQW--DVWTALETYLQLQEGFGWEPFKQLFKDYQSMSGISND--NKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEETRSKLSALPVWEKD 894          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045084.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 132.494 bits (332), Expect = 1.199e-30
Identity = 103/385 (26.75%), Postives = 176/385 (45.71%), Query Frame = 3
Query: 1956 DETSQLGLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDK--VDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWAS-----DRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPD 3083
            D     GLYL P K     F  S  Q  + + +     D+   ++Y + PV  R   +   ++    F +GGLL I    +++   + +   G    PV+ +  ++ +     ++   APWA L  ++ +  V  + +R+L+ P  +L L+D ++     L  I     R E    D+QI AG+MHAGYPI   ++        +K L D+  M   G WG +HE+GHN Q  ++ F   T + T N++++   E++ G     +N+   S        YI  +V   N    ++W  + + AL  Y QL+  FGWE + ++F+ Y + +       +  K+  W    S   + NL P ++ W WPI+  T  +L   P +  D
Sbjct:  527 DAWRSTGLYLAPRKTAVLEFPASVVQQGLKVQVGCQADDLSSAEKYCRAPVVVRKCHVDRQKVSVSCF-WGGLLYIIVKANSNLGIIPVKVYGAEPAPVYIKGKTNLDSWLQSVRYLPAPWAELITENIILTVPSDAIRSLSDPEALLSLWDKIMVSITELAAIPKTFPRPERFVADVQISAGWMHAGYPIMCHLE-------SAKELTDLNVMQTVGIWGPIHELGHNQQKINWEFPPHTTEATCNLWSVYVHETVLGI---PRNKAHGSLQPETRACYIQEYVK--NGSNLEQW--DVWTALETYLQLQEGFGWEPFKRLFKDYQSMSGISND--NKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEATRIKLSALPVWEKD 894          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Match: ENSXETT00000045039.1 (TRPM8 channel-associated factor 2 [Source:NCBI gene;Acc:100170200])

HSP 1 Score: 131.724 bits (330), Expect = 1.960e-30
Identity = 103/385 (26.75%), Postives = 176/385 (45.71%), Query Frame = 3
Query: 1956 DETSQLGLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDK--VDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWAS-----DRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPD 3083
            D     GLYL P K     F  S  Q  + + +     D+   ++Y + PV  R   +   ++    F +GGLL I    +++   + +   G    PV+ +  ++ +     ++   APWA L  ++ +  V  + +R+L+ P  +L L+D ++     L  I     R E    D+QI AG+MHAGYPI   ++        +K L D+  M   G WG +HE+GHN Q  ++ F   T + T N++++   E++ G     +N+   S        YI  +V   N    ++W  + + AL  Y QL+  FGWE + ++F+ Y + +       +  K+  W    S   + NL P ++ W WPI+  T  +L   P +  D
Sbjct:  527 DAWRSTGLYLAPRKTAVLEFPASVVQQGLKVQVGCQADDLSSAEKYCRAPVVVRKCHVDRQKVSVSCF-WGGLLYIIVKANSNLGIIPVKVYGAEPAPVYIKGKTNLDSWLQSVRYLPAPWAELITENIILTVPSDAIRSLSDPEALLSLWDKIMVSITELAAIPKTFPRPERFVADVQISAGWMHAGYPIMCHLE-------SAKELTDLNVMQTVGIWGPIHELGHNQQKINWEFPPHTTEATCNLWSVYVHETVLGI---PRNKAHGSLQPETRACYIQEYVK--NGSNLEQW--DVWTALETYLQLQEGFGWEPFKRLFKDYQSMSGISND--NKSKMNLWAEKFSEAVQTNLIPFFEAWGWPIEEATRIKLSVLPVWEKD 894          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf3 (TRPM8 channel-associated factor 3 [Source:MGI Symbol;Acc:MGI:3042585])

HSP 1 Score: 142.124 bits (357), Expect = 7.349e-34
Identity = 107/371 (28.84%), Postives = 171/371 (46.09%), Query Frame = 3
Query: 1974 GLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCA--LDNDKVDMVFSGVIQVPVFDRRDSDNNKNW-NVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTF-TGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYLP G++   L  +  +   + + +  H  +I Q   Y++ PV      L+ ++  + S+ +GGLL I      + D V +   G +  P F R      + W N++  SKAPW  LA D+ +  +    ++ L  P+ +L+L+D MV+    L       +R E +  D QI  G++H+GYPI  +I  V      S+F      +   G WGV+HE+GHN Q S +TF   T +   N++T+   E++       Q     +      R   +LN+G     W    + AL  Y QL+  FGWE + QVF  Y  R +      + +K+  W+   S     NL P ++ W WP+     + L   P
Sbjct:  539 GLYLPEGQVAQVLLPSEATHAKLKVLIGCHRDNISQARTYFRPPVMTYVYHLTSSQT-SISWLYGGLLYIMVPNKYNQDNVSVTIRGAVSAPYF-RLGKTTQEEWKNLITHSKAPWGELATDNIILTIPTVNLKELQDPYPLLQLWDKMVRAVAKLAARPFPFQRAERVVLDKQISFGFLHSGYPIMGLISIVEG--IISEF-----KIRSHGIWGVIHELGHNHQKSGWTFPPHTTEALCNLWTIYVHETVLNIPR-EQAHPSLNPELRRQRIKYHLNKGAPLSNWIM--WTALETYLQLQEGFGWEPFIQVFADY--RTLSGLPQNNEDKMNLWVKKFSEAVHKNLAPFFEAWGWPVKYAVAKSLASLP 895          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf2 (TRPM8 channel-associated factor 2 [Source:MGI Symbol;Acc:MGI:2385258])

HSP 1 Score: 130.568 bits (327), Expect = 3.573e-30
Identity = 98/379 (25.86%), Postives = 174/379 (45.91%), Query Frame = 3
Query: 1953 KDETSQLGLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSI----SCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            +D     GLYL  G+    ++ + + S    + +  H  D+    + ++ PV      ++  + ++ S  +GGLL I     C L    V +  +  +  P +    +   +  + +Q++  PW  LA D+ +  V   +++ L  P  +L+L+D M++    L       +R E I  D+Q+ AG+MH+GYPI   ++ V         L  + N+  +G WG +HE+GHN Q   + F   T + T N++++   E++ G    +Q            R   +L +G     W    + AL  Y QL+  FGWE +  +F +Y  + IF     +  K+  W+ L S   + NL P ++ W WPI  +  ++L  YP
Sbjct:  542 RDVWMSTGLYLLEGQSTEISVSEPAASAGLKVQIGCHTDDLTFAIKLFRAPVVTYQCCMNRTQ-RSVSCLWGGLLYIIVPKGCQLG--PVSVTITNAVPAPYYKLGKTSLEEWKSCIQKNLGPWGELATDNVILTVPTASLKTLENPEPLLQLWDEMMQAVARLASQPFPFQRPERIVADVQLSAGWMHSGYPIMCHMESVQE-------LVSLANIRSKGLWGPIHELGHNQQCRGWEFPPHTTEATCNLWSVYVHETVLGIPR-AQAHPQLKPEEREKRIKEHLQKGAPLQNWNV--WTALETYLQLQEVFGWEPFITLFAEY--QTIFYIPEDNECKMNIWLKLFSEKVQKNLVPFFEAWGWPIQKDVAEDLACYP 905          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf1 (TRPM8 channel-associated factor 1 [Source:MGI Symbol;Acc:MGI:1914665])

HSP 1 Score: 125.946 bits (315), Expect = 9.036e-29
Identity = 94/372 (25.27%), Postives = 163/372 (43.82%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEG-WASDRSYINRFVTYLNEG-KFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYD-TEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLY+P  + I  ++ +++ S    + +  H  D+ +  + ++ P+      L     ++ +  +GGLL I    ++    V +   G ++ P +   ++   +    L     PW  LA D+ +  V    +R L  P  +L L+D M++    L G     +  + I  D+QI  G+MHAGYPI   ++ V         L +   +  +G WG +HE+G N Q   + F   T + T N++ +   E++ G      N   W   R    R   YL++G     W    + AL  Y QL+  FGWE + ++F +Y  +        D  +K+  W+ + S   + NL P ++ W WPI       L   P
Sbjct:  548 GLYIPGRQVIDVSVPESAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLD-KPTKSITCLWGGLLYIIVPQNSKLGSVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEMMQAVAKLGGETFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQE-------LINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVRE--KRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf1 (TRPM8 channel-associated factor 1 [Source:MGI Symbol;Acc:MGI:1914665])

HSP 1 Score: 125.946 bits (315), Expect = 9.036e-29
Identity = 94/372 (25.27%), Postives = 163/372 (43.82%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEG-WASDRSYINRFVTYLNEG-KFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYD-TEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLY+P  + I  ++ +++ S    + +  H  D+ +  + ++ P+      L     ++ +  +GGLL I    ++    V +   G ++ P +   ++   +    L     PW  LA D+ +  V    +R L  P  +L L+D M++    L G     +  + I  D+QI  G+MHAGYPI   ++ V         L +   +  +G WG +HE+G N Q   + F   T + T N++ +   E++ G      N   W   R    R   YL++G     W    + AL  Y QL+  FGWE + ++F +Y  +        D  +K+  W+ + S   + NL P ++ W WPI       L   P
Sbjct:  548 GLYIPGRQVIDVSVPESAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLD-KPTKSITCLWGGLLYIIVPQNSKLGSVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEMMQAVAKLGGETFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQE-------LINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVRE--KRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Match: Tcaf1 (TRPM8 channel-associated factor 1 [Source:MGI Symbol;Acc:MGI:1914665])

HSP 1 Score: 125.946 bits (315), Expect = 9.036e-29
Identity = 94/372 (25.27%), Postives = 163/372 (43.82%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDN--DKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEG-WASDRSYINRFVTYLNEG-KFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYD-TEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLY+P  + I  ++ +++ S    + +  H  D+ +  + ++ P+      L     ++ +  +GGLL I    ++    V +   G ++ P +   ++   +    L     PW  LA D+ +  V    +R L  P  +L L+D M++    L G     +  + I  D+QI  G+MHAGYPI   ++ V         L +   +  +G WG +HE+G N Q   + F   T + T N++ +   E++ G      N   W   R    R   YL++G     W    + AL  Y QL+  FGWE + ++F +Y  +        D  +K+  W+ + S   + NL P ++ W WPI       L   P
Sbjct:  548 GLYIPGRQVIDVSVPESAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLD-KPTKSITCLWGGLLYIIVPQNSKLGSVPLTVKGAVRAPFYKLGETSKEEWKRRLLEYPGPWGELATDNIILTVPTANLRTLENPEPLLRLWDEMMQAVAKLGGETFPLRLPQRIVADVQISVGWMHAGYPIMCHLESVQE-------LINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCNLWCVYVHETVLGIPRSRANIALWPPVRE--KRVRIYLSKGPSVKHWNA--WTALETYLQLQEAFGWEPFIRLFTEYRNQTTSSSPPSDNVDKMNLWVKMFSQQVQRNLAPFFEAWGWPIQKEVATSLAYLP 907          
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6QR59|TCAF3_MOUSE (TRPM8 channel-associated factor 3 OS=Mus musculus OX=10090 GN=Tcaf3 PE=1 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 5.140e-33
Identity = 107/371 (28.84%), Postives = 171/371 (46.09%), Query Frame = 3
Query: 1974 GLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCA--LDNDKVDMVFSGVIQVPVFDRRDSDNNKNW-NVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTF-TGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYLP G++   L  +  +   + + +  H  +I Q   Y++ PV      L+ ++  + S+ +GGLL I      + D V +   G +  P F R      + W N++  SKAPW  LA D+ +  +    ++ L  P+ +L+L+D MV+    L       +R E +  D QI  G++H+GYPI  +I  V      S+F      +   G WGV+HE+GHN Q S +TF   T +   N++T+   E++       Q     +      R   +LN+G     W    + AL  Y QL+  FGWE + QVF  Y  R +      + +K+  W+   S     NL P ++ W WP+     + L   P
Sbjct:  539 GLYLPEGQVAQVLLPSEATHAKLKVLIGCHRDNISQARTYFRPPVMTYVYHLTSSQT-SISWLYGGLLYIMVPNKYNQDNVSVTIRGAVSAPYF-RLGKTTQEEWKNLITHSKAPWGELATDNIILTIPTVNLKELQDPYPLLQLWDKMVRAVAKLAARPFPFQRAERVVLDKQISFGFLHSGYPIMGLISIVEG--IISEF-----KIRSHGIWGVIHELGHNHQKSGWTFPPHTTEALCNLWTIYVHETVLNIPR-EQAHPSLNPELRRQRIKYHLNKGAPLSNWIM--WTALETYLQLQEGFGWEPFIQVFADY--RTLSGLPQNNEDKMNLWVKKFSEAVHKNLAPFFEAWGWPVKYAVAKSLASLP 895          
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5XHI4|TCAF_XENLA (TRPM8 channel-associated factor homolog OS=Xenopus laevis OX=8355 GN=tcaf PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 2.366e-32
Identity = 107/391 (27.37%), Postives = 178/391 (45.52%), Query Frame = 3
Query: 1974 GLYLPPGKIGTALFKNSK-SQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDR-------RDSDNNKNW-NVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTF-TGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNK 3113
            GLYL P K     F  S   Q   + +     D+   D+Y + PV  R   +    +    F +GGL+ I+   +++       G+I V V++        +   +   W   ++   APWA L  ++ +  V  + +R+L+ P  +L L+D ++     L  I  +  R E    D+QI AG+MHAGYPI   ++        +K L D+  M   G WG +HE+GHN Q +++     T + T N++++   E++ G    SQ        +  N    YL  G   ++W    + AL  Y QL+  FGWE + Q+F+ Y + +    R  +  K+  W    S   + NL P ++ W WPI+  T+ +L   P +  D   + L + K
Sbjct:  530 GLYLAPRKTAVLEFPASAVHQGLQVQVGCQSDDLSSADKYCRAPVVVRRFHVDSQRVSVSCF-WGGLVYITVKANSNL------GIIPVKVYEAEPAPIYIKGKTSLDTWIQSIRNLPAPWAELITENIILTVPSDAIRSLSDPEALLSLWDKIMVAITELAAIPKKLPRPERFVADVQISAGWMHAGYPIMCHLE-------SAKELTDLNIMQTGGIWGPIHELGHNQQKTNWELPPHTTEATCNLWSVYVHETVLGIPR-SQAHCCLQAETRANHIQEYLRNGSNIEQWN--VWTALETYLQLQEGFGWEPFKQLFKDYQSMSGI--RNENKSKMNLWAEKFSEAVQTNLVPFFEAWGWPIEEATHSKLSVLPVWEKDPMKSYLSARK 901          
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|A6QLU7|TCAF2_BOVIN (TRPM8 channel-associated factor 2 OS=Bos taurus OX=9913 GN=TCAF2 PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 1.442e-31
Identity = 102/372 (27.42%), Postives = 164/372 (44.09%), Query Frame = 3
Query: 1974 GLYLPPGKIG-TALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSI----SCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GL LP G++    L + +      L +  H  ++    +  + PV      +   E Q  S  +GGLL +     C L    + +     +  P +   ++      + +Q S APW  LA D+ +  V    +R L  P  +L L+D M++    L       +R E I  D+QI AG+MH+GYPI   ++ V+        L D   M   G WG +HE+GHN Q   + F   T + T N++++   E++ G    +Q     S     NR  T+L +G    +WK   + AL  Y QL+  FGWE + Q+F +Y   +       +  K+  W+   S   + NL P ++ W WP++      L   P
Sbjct:  547 GLNLPNGQLTEVCLCEAAACAGLKLQIGCHTDNLMSASKLSRAPVVTHQCHMDRTE-QLVSNLWGGLLYVIVPTGCNLG--PMSITIKRAVPAPYYKLGETSLEAWRSCIQESPAPWGELATDNIILTVPTADLRALEDPEPLLRLWDEMMEAIARLAAQPFPFRRPERIVADVQISAGWMHSGYPIMCHLESVSE-------LIDETGMRSRGLWGPVHELGHNQQREQWEFPPHTTEATCNLWSVYVHETVLGIPR-AQAHPALSPPERENRIKTHLEKGAPLCDWKV--WTALETYLQLQEAFGWEPFTQLFAEYQTLSDIPND--NPGKMNLWVRKFSEKVQKNLAPFFEAWGWPVEKEVASSLACLP 903          
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6P6V7|TCAF3_RAT (TRPM8 channel-associated factor 3 OS=Rattus norvegicus OX=10116 GN=Tcaf3 PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 9.959e-31
Identity = 103/371 (27.76%), Postives = 166/371 (44.74%), Query Frame = 3
Query: 1974 GLYLPPGKIGTALFKNSKSQTCV-LYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCA--LDNDKVDMVFSGVIQVPVFDRRDSDNNKNW-NVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTF-TGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYLP G++         +   + + +  H+ +I Q   Y + PV      L+ ++  + S+ +GGLL I      + D V +  SG +  P F R      + W N+++ SKAPW  LA D+ +  +    ++ L  P+ +L+L+D +VK    L       +R E I  D QI  G++H+GYPI  +I  V         + +   +   G WGV HE+GHN Q   +TF   T +   N++++   E++       Q     +      R   +LN+G     W    + AL  Y QL+  FGWE + Q+F  Y       +   D  K+  W+   S   + NL P +  W WP+     + L   P
Sbjct:  539 GLYLPEGQVAQVTLPTEATHAKLKVLIGCHIDNISQAKTYIRPPVMTYVYHLTSSQT-SISWLYGGLLYIMVPDKYNQDNVSVTISGAVSAPYF-RLGKTTQEEWKNLIEHSKAPWGELATDNIILTIPTVNLKVLQDPYPLLQLWDKIVKAVAKLAARPFPFQRPERIVLDKQISFGFLHSGYPIMGLIIIVEG-------IINEFKIRSHGVWGVTHELGHNHQKPGWTFRPHTTEALCNLWSIYVHETVLNIPR-DQAHPSLNPELRKQRIKDHLNKGAPLSNWIV--WTALETYLQLQEGFGWEPFIQLFANYQTLTGLPQNNED--KMNLWVKKFSEVVQKNLAPFFKAWGWPVQHAVAKSLASLP 895          
BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Match: sp|A6NFQ2|TCAF2_HUMAN (TRPM8 channel-associated factor 2 OS=Homo sapiens OX=9606 GN=TCAF2 PE=1 SV=2)

HSP 1 Score: 133.65 bits (335), Expect = 2.573e-30
Identity = 100/370 (27.03%), Postives = 165/370 (44.59%), Query Frame = 3
Query: 1974 GLYLPPGK-IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            GLYL  G+    +L + + S    + +  H  D+ +  +  + PV      +   E ++ S  +GGLL +     +    V +   G +  P +    +   +    +Q + APW  LA D+ +  V    ++ L  P  VL L+D M++    L       +R E I  D+QI AG+MH+GYPI   ++ V       K + +  +M   G WG +HE+GHN Q   + F   T + T N++++   E++ G      +E   S      R   +L +G    +W    + AL  Y QL+  FGWE + Q+F +Y   +   K   +T ++  W+   S   K NL P ++ W WPI       L   P
Sbjct:  548 GLYLLEGQNAEVSLSEAAASAGLRVQIGCHTDDLTKARKLSRAPVVTHQCWMDRTE-RSVSCLWGGLLYVIVPKGSQLGPVPVTIRGAVPAPYYKLGKTSLEEWKRQMQENLAPWGELATDNIILTVPTTNLQALKDPEPVLRLWDEMMQAVARLAAEPFPFRRPERIVADVQISAGWMHSGYPIMCHLESV-------KEIINEMDMRSRGVWGPIHELGHNQQRHGWEFPPHTTEATCNLWSVYVHETVLGIPRAQAHEA-LSPPERERRIKAHLGKGAPLCDWNV--WTALETYLQLQEAFGWEPFTQLFAEYQTLSHLPKD--NTGRMNLWVKKFSEKVKKNLVPFFEAWGWPIQKEVADSLASLP 904          
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: V3ZHF7 (Peptidase M60 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_236481 PE=4 SV=1)

HSP 1 Score: 237.654 bits (605), Expect = 3.867e-62
Identity = 152/501 (30.34%), Postives = 249/501 (49.70%), Query Frame = 3
Query: 1710 ITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSD---NFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTC--VLYLNFHLFDIYQKDEYYKWPV--SKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPA-KLEEMI 3188
            + K+ +++ +I S      IP   + V+    +++++ +  +L    NG    PG+  +PG+   D   +  ++I++  +           G Y+  G+         +S  C   + +  H   +   +   +WP+   K+ I     +M+T   P GGL+ +       K+ +     I+ P+FD    +   NW   +++   WA L G   VF +   +V +L  P  VLE +D +V   +HLRG    S+RRE I  D+Q   GYMH+GYPI T +  V +PS KS FL +   ++  G WG+ HE+GHN+Q  ++TF GT +VT NIFTL  ++ +  KS W  +  W   +    R     + G F +W+++P +AL VYAQL + FGW +Y + FRQY  + +  +   + EKI  W    S  ++ NL PL D W  P+     ++L  +P F PDD +T+ V  KV+++  KY   +   R P  + EE I
Sbjct:  241 MKKSGEKVHKIISEKEHECIPSEKRPVSSSIGKSVMFLHD-VLAKTGNGYKKMPGIDLFPGDFTEDPPLSDGRIIIESEI-----PAAHPTGFYVRAGEEVELEIVTEESLNCGWEIQIGCHSDHLQTSNAMKRWPIITEKQKITQKNLKMKT---PIGGLMYVWSPKMASKIIINVKNAIESPLFDLTQPETIINWKTNRQAPGLWADLGGHHIVFTLPAKSVGDLEDPTAVLESWDRVVCAHHHLRGTDPNSQRREWIVADVQPSCGYMHSGYPIVTHLD-VADPS-KSSFLLNNKTLLESGNWGLFHELGHNMQKPTWTFRGTGEVTCNIFTLHAMDVIAQKSPWIHS--WLEGKVPKARQFIRSSAG-FSKWQSDPGIALFVYAQLYNQFGWSVYKKTFRQYEEQDMPPQLKTEEEKIDYWFETMSKFSEYNLAPLADLWSIPLSDTCREQLKVFPSFLPDDEITQGVPEKVEELEKKYPKLVRKIRPPCDQFEEQI 727          
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S3I0H4 (TRPM8 channel-associated factor 2-like OS=Lingula unguis OX=7574 GN=LOC106159184 PE=4 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 2.002e-58
Identity = 142/460 (30.87%), Postives = 224/460 (48.70%), Query Frame = 3
Query: 1839 IYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKI---------GTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYK----SKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQY--NARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAK 3173
            + P       PG+  +PG+  S    Q  V++ L  K +   S  G Y+P G+          GT L   S  + C+   +  L ++  K+ + +WP    ++L  +      S P+GGLL       N ++ ++   V++ P FD    +  ++W+  + +   WA LAG   VF V   +VR +  P  +L L+D  V  ++ LRG     ++RE +  D Q  AGYMHAGYPI T +  V +P+++      F+F    M   G WG+ HEIGHN+Q  ++TF+G  +VTNNIFTL   E++TG  +W+           + R +   ++    +WK +PF AL VY+QL   FGW  +  VFR Y  + +        D  KI  W    S   + NL P+ DFW  P+ +     L  +P F P+D +T     +VD+++  Y G   + R P++
Sbjct:  276 MTPPQAFCKLPGIGVFPGDFSSKPPLQ-TVRLNLTTKHRQRLST-GYYVPAGQPVKVTVTSDQGTDL---SGWKICIGAHDDSLVNV--KEPWRRWP--DVAVLEELKATTALSSPYGGLLFFDSPERNAELTVIVCNVVEAPFFDLTKPEIVEDWSRRRNAPGLWAELAGRHIVFTVPSTSVREIDDPTTILALWDSAVAAQHDLRGTDRNLQKRERVVADEQPSAGYMHAGYPIVTHLD-VVDPNFQYNGSDNFVFSESGMKSYGNWGLFHEIGHNMQRKAWTFSGMGEVTNNIFTLYTYEAVTGNDAWNNPTMKKFRAEKLPRLLQ--SQPSLSDWKKDPFFALCVYSQLAHEFGWRSFKTVFRLYENSVKKEEESNQDDGAKIDMWFGRFSEIVRHNLSPMCDFWGIPLSTGVRNNLTLFPAFLPNDEITAAGQARVDQVLPNYPG---IVRSPSR 720          
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: R7UHN9 (Peptidase M60 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_195748 PE=4 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 5.042e-57
Identity = 190/731 (25.99%), Postives = 342/731 (46.79%), Query Frame = 3
Query: 1050 NIISMPSCSNFGSFLILKDNAFPYAITSD-NDVVFVISEIGKGRIGLIACDTITTDYVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGI-----RMTMQLIWNAH-YEI------------------EESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSEL--VTLFKASGVWPSLKTINGD---LKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGK-IGTALFKNSKS-QTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGG 3143
            ++  +P     G+ +   D A P     D +DVV   +E+G GR+  IA        +  + YLK+ +G ++        D I+KL  +  K +     +  + L  NA  +EI                  +E  V  L+D I++G  ++  + PW +    KN    ++   ++ + +G+  +   ING+     + +  + A  P   L++   ++  NL   +  + +++   P +I+  R  F ++   ++E  + Y+      IP     + +   + L+    + L     G   APGV  +PG+ + D  +   V++ L+   +D      +YLP G+ +   + + S   Q   +++  H  D+ +  ++ + P       LS +    FS P+GG +      +  ++D+  S V++ P +         +W   + +   WA LAG   +  +  +++++L  P +VL  +D +V   + +R   V S R++ I  D+Q   GYMH+GYPI T +  V +P+   +FL +   +  EG WG+ HE+GHN+Q   +TF GT +VT N+FTL  ++ + G   W     W +++  +N    YL  G  F  WK EP +ALG+YAQL  + GW  Y  VFR+Y   +   K   D EKI+ WI   +     NLC L+ FW + I ++  + L + P F P D +T  V  ++ K++ ++ G
Sbjct:   11 DLTVIPKIGTPGTLVTYGDQASPVLWGEDPSDVVIGAAELGAGRV--IA--------LTHESYLKS-FGSHE-------NDDIRKLQANLKKWVSFGREKDVVHLKNNASIHEILAKETESVAIVRIIGQKFDEIEVLMLLDFIEHGGGLIFAICPWGWLQLNKNYAEEDMPMYSILERAGIHFTGHYINGNADGFTVPSIPSTARVPT--LIQKCLSSSENLGLYAYDLASLLEDLPLRIV--RSTFQDLLDEVEE-TDFYN----LVIPEEKYPLKDNRSKGLLLL--SCLSARVRGDK-APGVHAFPGDFEGD-VQHKCVRINLDSARRD-IFPAAVYLPAGETLEIEIHEGSTDPQKWKVWIGAHSDDLVRSSQWKRLPKVHIKQRLSEDTNVIFS-PYGGNVYFESPDEESQLDVTVSNVVESPCY-------TDHWIGQRDAPGLWADLAGRHIMITLPSSSIKDLVDPSEVLAFWDSVVAAHHVVRTTDVSSHRKQWIVCDVQPVVGYMHSGYPIVTHMD-VADPT-SDQFLLNHDKLKAEGNWGLYHELGHNMQRLEWTFQGTSEVTVNVFTLHAMDVVNGVKPWIHP--WLNNQ--MNAIKRYLMAGADFTTWKKEPGIALGIYAQLAHHLGWGTYKAVFRKYEVMSNEQKPKTDDEKIRLWITTFAECCGQNLCALFRFWGFTIPNDLQRNLAELPLFLPMDEMTLFVPERIAKVVAEFDG 695          
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: V4A0M8 (Peptidase M60 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_165593 PE=4 SV=1)

HSP 1 Score: 225.713 bits (574), Expect = 5.090e-57
Identity = 135/443 (30.47%), Postives = 235/443 (53.05%), Query Frame = 3
Query: 1863 VAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGK--IGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTF-SFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGK----SSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPA 3170
            + PG++ +PG+ +S   +   V + L+  ++++    G Y+  G     + L +N K  T  + +  H   +  + E  +WP         +NE  T     FGGL+  +   +   + +    VIQ P+FD       + WN  +++   WA +  +  +  +   ++RNL  P  V++ +D ++K  +HL+GI +++ R++ + TD+Q  AGYMH+GYP+ T +  V NPS++  FL +  N+  +G WGV HE GHN+Q+  +TF GT +VT NIFTL  +++L GK      W QN+      S   R++ Y N+  +  WK++P +AL +YAQ+  + GW ++ +VFR+Y           + ++I  W  L S  +++NL  L DFW+ P+     +E+ K+  F PDD + ++   +   I+ KY  + +  RQPA
Sbjct:  505 ILPGIANFPGDFESTPSKT-SVTLTLS-SYQEDVHPTGYYVQAGDSLAVSVLSQNGKGWT--VQIGAHSDCLSGQSELKRWP--NVVCCKPLNEQCTIVKATFGGLVYFTSPSNQQSITVQLDNVIQAPLFDLTLPTTIEQWNGRRQAPGLWADICSEFLIITLPSKSIRNLDGPVSVMKKWDEVLKAHFHLKGIDIRNHRKQWLVTDVQPSAGYMHSGYPVVTHLD-VANPSHEF-FLLNSHNLNTKGSWGVFHEFGHNMQEGVWTFPGTTEVTVNIFTLHAMDTLIGKRPIVHEWLQNQ-----MSNAKRYL-YHNDQNYSHWKSDPGIALFIYAQIAHHLGWGVFKEVFRRYGRMNPSSYPNKENDQIDLWYTLLSEVSQLNLASLVDFWKIPLSDKARREMSKFQPFLPDDDVMRMAPERTHMILAKYHSNCL--RQPA 931          
BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Match: A0A1S3KG34 (TRPM8 channel-associated factor 2 OS=Lingula unguis OX=7574 GN=LOC106181552 PE=4 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 1.774e-56
Identity = 136/458 (29.69%), Postives = 225/458 (49.13%), Query Frame = 3
Query: 1839 IYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETS-QLGLYLPPGK-IGTALFKNSKS-----QTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYK----SKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQY--NARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAK 3173
            + P       PG+  +PG+    +F+  +  + LNL  K       G Y+P G+ +  A+  +  +     + C+   +  L ++  K+ + +WP    ++L  +      S P+GGLL       N ++ ++   V++ P FD    +  ++W+  + +   W  LAG   VF V   +VR +  P  +L L+D  V  ++ LRG     ++RE +  D Q  AGYMHAGYPI T +  V +P+++      F+F    M + G WG+ HEIGHN+Q  ++TF+G  +VTNNIFTL   E++TG  +W+           + R +    +   ++WK +PF AL VY+QL   FGW  +  VFR Y  + +        D  KI  +    S   + NL P+ DFW  P+ +     L  +P F P+D +T     +VD+++  Y G   + R P++
Sbjct:  276 MTPPQAFCKLPGIDVFPGDF---SFKPPLQTVRLNLTTKHRQRLSTGYYVPAGQPVKVAVTSDQGTDLSGWKICIGAHDDSLVNV--KEPWRRWP--DVAVLEELKATTALSSPYGGLLFFDSPERNAELTVIVCNVVEAPFFDLTKPEIVEDWSRRRNAPGLWTELAGRHIVFTVPSTSVREIDDPTTILALWDSAVAAQHDLRGTDPNFQKRERVVADEQPSAGYMHAGYPIVTHLD-VVDPNFQYNGSDNFVFSESGMKLYGNWGLFHEIGHNMQRKAWTFSGMGEVTNNIFTLYTYEAVTGNDAWNNPTMKKFRAEKLPRLLQ--TQPSLNDWKKDPFFALCVYSQLAHEFGWGSFKTVFRLYENSVKKEEESNQDDGAKIDMYFGRFSEIVRHNLSPMCDFWGIPLSTGVRNNLTLFPAFLPNDEITAAGQARVDQVLPNYPG---IVRGPSR 720          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: si:ch211-210b2.3 (si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-13])

HSP 1 Score: 116.316 bits (290), Expect = 7.398e-26
Identity = 101/392 (25.77%), Postives = 170/392 (43.37%), Query Frame = 3
Query: 1926 VQMALNLKFKDETSQLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSI----SCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNW-NVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPI---GTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTF-TGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            ++++ N    +E    GLYL PG +   A+      +   L +      +   D   + PV      L  + +Q ++  +GGL+ +     C LD   V+++    I+ P +   ++ +  NW N ++ + APWA L  ++ +  +    +R L +P +V                      R+E    D+QI  G+MHAGYPI         + NP    KF          G WG +HE+GHN Q  ++ F   T + T N++++   E + G +  S +E  + +R +  R  +Y   G+  D+W    + AL  Y QL+  FGW+ + +VF  Y+   I      +  K+  +    S    MNL P +  W WPI  +T   L   P
Sbjct:  530 IRVSANTGDGEEWISTGLYLSPGMRTFVAVPAEIMDKGWQLQIGCQTDHLGHADVLRRAPVVHERFALDKDMLQVWNL-WGGLIYLIAPPKCKLDG--VEVIVQTAIKAPYYKSGET-SVANWMNEIRNAPAPWAELEFENIIITLQSEVIRQLDRPDEV-------------------AFPRKERFVADVQISHGFMHAGYPIMILSCSAPELVNPESARKF----------GIWGAIHELGHNQQRWAWEFPPNTTECTCNLWSVYVHEEVLGVNRASAHENMSPERRW-GRAQSYAMGGRQLDQWSM--WTALETYMQLQDRFGWDAFKKVFTAYH--TINNVPGDNNGKMNLYAETFSKVVNMNLAPFFKAWGWPIQPSTEDALSSLP 883          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000018418.2 (plasminogen [Source:NCBI gene;Acc:103036400])

HSP 1 Score: 107.842 bits (268), Expect = 2.432e-23
Identity = 63/166 (37.95%), Postives = 89/166 (53.61%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF--LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQ----NKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            G++Y G   KT SG  C +W      + N+  +     DLES    NFCRNP+    GPWC T + A +WE+CDI  C ++   C     G+ Y G ++ TESG  C+ W  Q    + +  + FPE  L+  ENYCR+PDG  K PWC+ +    +W+ C V +C
Sbjct:  116 GTDYRGTKSKTKSGTLCQRWESTFPHKPNITPRSHPKADLES----NFCRNPDGDKGGPWCYTTDPAKRWENCDIQDCTEECMHC----SGENYRGKISTTESGLTCQRWDSQKPHNHGYMPSVFPEKYLE--ENYCRNPDGEPK-PWCFTESPAKRWEFCSVSRC 270          

HSP 2 Score: 91.6633 bits (226), Expect = 2.588e-18
Identity = 62/195 (31.79%), Postives = 87/195 (44.62%), Query Frame = 3
Query:  126 HGSEYVGNVEKTFSGKKCIKW-TIELLRRLNLEEK-HFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDN--------------------------NWCKDTAEGQEYFGSLNLTESGKDCKLWSLQ----NKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEI 614
            +GS Y G + +T SGKKC  W ++E    +   +     DL      N CRNP+   + PWC T + +++WE+C I  C                                CK T  G  Y GS++ T +G  C+ WS      + F     P+  L +  N CR+PDG   GPWCY  D N KWD C +P+C +
Sbjct:  381 NGSSYRGVMTETISGKKCQAWASMEPHSHVKTPQNFPTADLR----RNLCRNPDGD-RAPWCYTTDPSVRWEYCRIERCGSTTVSLTPPPALKPVVPPTLLTQSVPSEFADCK-TGSGISYRGSVSKTVAGVTCQAWSASTPHLSSFTPQAHPDKGLDS--NKCRNPDGDVNGPWCYTTDPNKKWDYCQIPECAL 567          

HSP 3 Score: 88.5817 bits (218), Expect = 2.273e-17
Identity = 59/183 (32.24%), Postives = 86/183 (46.99%), Query Frame = 3
Query:  117 ITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF----LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFC----------VKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQN-----KFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
             T  G+ Y G V  T SGK C  W  +L  + +    ++    LD       N+CRNP+   K PWC T +   +WE+C++  C          +  N  C +   G  Y G +  T SGK C+ W+        K P N FP   L+   N CR+PDG  + PWCY  D +++W+ C + +C
Sbjct:  284 TTGDGNTYRGTVSVTESGKTCQSWASQLPHKHSRTPDNYPCKGLD------QNYCRNPDNERK-PWCYTTDPETRWEYCNVESCGNEPRPDEPVIAPNEDCYED-NGSSYRGVMTETISGKKCQAWASMEPHSHVKTPQN-FPTADLR--RNLCRNPDG-DRAPWCYTTDPSVRWEYCRIERC 454          

HSP 4 Score: 55.4546 bits (132), Expect = 3.375e-7
Identity = 34/103 (33.01%), Postives = 49/103 (47.57%), Query Frame = 3
Query:   78 LSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLD--LESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCK 380
            L+ +  +E   CK T  G  Y G+V KT +G  C  W+       +   +   D  L+S    N CRNP+    GPWC T +   +W++C IP C  D  + K
Sbjct:  476 LTQSVPSEFADCK-TGSGISYRGSVSKTVAGVTCQAWSASTPHLSSFTPQAHPDKGLDS----NKCRNPDGDVNGPWCYTTDPNKKWDYCQIPECALDTEYLK 573          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000018419.2 (plasminogen [Source:NCBI gene;Acc:103036400])

HSP 1 Score: 107.457 bits (267), Expect = 2.841e-23
Identity = 63/166 (37.95%), Postives = 89/166 (53.61%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF--LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQ----NKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            G++Y G   KT SG  C +W      + N+  +     DLES    NFCRNP+    GPWC T + A +WE+CDI  C ++   C     G+ Y G ++ TESG  C+ W  Q    + +  + FPE  L+  ENYCR+PDG  K PWC+ +    +W+ C V +C
Sbjct:  105 GTDYRGTKSKTKSGTLCQRWESTFPHKPNITPRSHPKADLES----NFCRNPDGDKGGPWCYTTDPAKRWENCDIQDCTEECMHC----SGENYRGKISTTESGLTCQRWDSQKPHNHGYMPSVFPEKYLE--ENYCRNPDGEPK-PWCFTESPAKRWEFCSVSRC 259          

HSP 2 Score: 92.8189 bits (229), Expect = 1.157e-18
Identity = 63/194 (32.47%), Postives = 87/194 (44.85%), Query Frame = 3
Query:  126 HGSEYVGNVEKTFSGKKCIKW-TIELLRRLNLEEK-HFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDN--------------------------NWCKDTAEGQEYFGSLNLTESGKDCKLWSLQ----NKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCE 611
            +GS Y G + +T SGKKC  W ++E    +   +     DL      N CRNP+   + PWC T + +++WE+C I  C                                CK T  G  Y GS++ T +G  C+ WS      + F     P+  L +  N CR+PDG   GPWCY  D N KWD C +P+CE
Sbjct:  370 NGSSYRGVMTETISGKKCQAWASMEPHSHVKTPQNFPTADLR----RNLCRNPDGD-RAPWCYTTDPSVRWEYCRIERCGSTTVSLTPPPALKPVVPPTLLTQSVPSEFADCK-TGSGISYRGSVSKTVAGVTCQAWSASTPHLSSFTPQAHPDKGLDS--NKCRNPDGDVNGPWCYTTDPNKKWDYCQIPECE 555          

HSP 3 Score: 88.1965 bits (217), Expect = 2.632e-17
Identity = 59/183 (32.24%), Postives = 86/183 (46.99%), Query Frame = 3
Query:  117 ITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF----LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFC----------VKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQN-----KFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
             T  G+ Y G V  T SGK C  W  +L  + +    ++    LD       N+CRNP+   K PWC T +   +WE+C++  C          +  N  C +   G  Y G +  T SGK C+ W+        K P N FP   L+   N CR+PDG  + PWCY  D +++W+ C + +C
Sbjct:  273 TTGDGNTYRGTVSVTESGKTCQSWASQLPHKHSRTPDNYPCKGLD------QNYCRNPDNERK-PWCYTTDPETRWEYCNVESCGNEPRPDEPVIAPNEDCYED-NGSSYRGVMTETISGKKCQAWASMEPHSHVKTPQN-FPTADLR--RNLCRNPDG-DRAPWCYTTDPSVRWEYCRIERC 443          

HSP 4 Score: 53.1434 bits (126), Expect = 1.603e-6
Identity = 32/95 (33.68%), Postives = 46/95 (48.42%), Query Frame = 3
Query:   78 LSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLD--LESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFC 356
            L+ +  +E   CK T  G  Y G+V KT +G  C  W+       +   +   D  L+S    N CRNP+    GPWC T +   +W++C IP C
Sbjct:  465 LTQSVPSEFADCK-TGSGISYRGSVSKTVAGVTCQAWSASTPHLSSFTPQAHPDKGLDS----NKCRNPDGDVNGPWCYTTDPNKKWDYCQIPEC 554          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: hgfb (hepatocyte growth factor [Source:NCBI gene;Acc:103033838])

HSP 1 Score: 103.99 bits (258), Expect = 3.421e-22
Identity = 59/177 (33.33%), Postives = 95/177 (53.67%), Query Frame = 3
Query:  102 IKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPH---NFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSL----QNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCE 611
            +K+C I + G  Y G + KT +G++C KW +         E HFL           N CRNP+    GPWC T    +++++C+IP C +       T  G+ Y G ++ TESG++C+ W L    ++ F    +P+  L+  +NYCR+PDG  + PWC+  D ++ W+ C + QC+
Sbjct:  109 VKECIIGT-GLNYKGKISKTETGEECQKWDLNTP-----HEHHFLPRRHKKKDLRDNLCRNPDNSTTGPWCFTKNPNIRYQNCNIPQCSEVECM---TCNGESYRGPMDHTESGRECQRWDLDEPHKHLFHPKRYPDNGLK--DNYCRNPDGRQR-PWCFTTDPDVPWEYCSIKQCD 273          

HSP 2 Score: 88.5817 bits (218), Expect = 1.710e-17
Identity = 54/181 (29.83%), Postives = 88/181 (48.62%), Query Frame = 3
Query:  117 ITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTA------EGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPE--MSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCY-VPQCEISFSQIE 632
            +T +G  Y G ++ T SG++C +W ++   +     K + D  +    N+CRNP+   + PWC T +  + WE+C I  C  DN    +T        G  Y G++ +   G  C+ W  Q     +  P+       +EN+CR+PDG    PWC+  D NI W  C  +P+C  + ++ E
Sbjct:  196 MTCNGESYRGPMDHTESGRECQRWDLDEPHKHLFHPKRYPD--NGLKDNYCRNPDGRQR-PWCFTTDPDVPWEYCSIKQCDSDNQSNIETTTTCFRDNGAGYRGTVAVPREGLTCQRWDAQFPHSHSYMPQKYRCKDLSENFCRNPDGRD-APWCFTTDPNIPWAFCKNIPRCGAAATEPE 372          

HSP 3 Score: 72.4034 bits (176), Expect = 1.735e-12
Identity = 62/230 (26.96%), Postives = 85/230 (36.96%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFL--DLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHC-DIPFCVKDNNWCKDTAEGQE--YFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFI-----VVIDLTKWSLYAQSRTTLWATG 788
            G+ Y G V     G  C +W  +     +   + +   DL      NFCRNP+     PWC T +  + W  C +IP C       ++  +G E  Y G    T SG  C  W   ++        +S +   N+CR+PD    GPWCY + S + WD C V  C+                 L     V Q    V     I     V I    W +  Q   T W  G
Sbjct:  291 GAGYRGTVAVPREGLTCQRWDAQFPHSHSYMPQKYRCKDLSE----NFCRNPDGR-DAPWCFTTDPNIPWAFCKNIPRCGAAATEPEECYKGSEETYRGKQMKTRSGILCAPWRDHSERNKREVVTLSEELEGNFCRNPDKDKHGPWCYTNSSLVPWDYCTVKLCD------------PLPNALPQKAEVSQASCFVHKQVRIVGGGPVPIKEGSWMVSIQKGATHWCGG 503          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000051129.1 (pep primary_assembly:Astyanax_mexicanus-2.0:1:5658439:5662219:-1 gene:ENSAMXG00000036822.1 transcript:ENSAMXT00000051129.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.7373 bits (221), Expect = 1.172e-19
Identity = 63/210 (30.00%), Postives = 101/210 (48.10%), Query Frame = 3
Query: 2454 RRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGR---WGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            R+E    D+QI AG+MH+GYPI  M+  V+ P        D+ N  + G+   WG +HE+GHN Q S + F   T + T N++++   E + G    +   G  + +   NR   Y+  G+   +W    + AL  Y QL+  FGW+ + +VF  Y+      +   +  K+  +    S     NL P +  W WPI  +T ++L   P
Sbjct:   16 RKERFVADVQILAGFMHSGYPI--MMHTVSAP--------DLVNPYVSGKSDFWGPVHELGHNQQHSDWEFPPYTTECTCNLWSVYVHEVVLGVKK-ADAHGQMTPQRRQNRIKKYIEGGRNLKDWTV--WTALETYMQLQEEFGWDAFKKVFAAYHDMTGVPQD--NKGKMNLYAETFSKVVNRNLTPFFKAWGWPIQPSTEEQLCSLP 210          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000976.1 (pep scaffold:Pmarinus_7.0:GL479898:35358:38300:-1 gene:ENSPMAG00000000885.1 transcript:ENSPMAT00000000976.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 129.028 bits (323), Expect = 1.621e-30
Identity = 125/478 (26.15%), Postives = 202/478 (42.26%), Query Frame = 3
Query: 1662 SISPFQI-MGSRDEFAEITKNIQ----ELIEIYSHHVKSFIPCL-DKTVTERTERNLIYFYSALLIYPFNGLMVAPGVS----FYPGNIQSDNFRQLIVQMALNLKFKDETS--QLGLYLPPG-KIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSF----PFGGLLS--ISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRS-KAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTG-TVDVTNNIFTLVGVESLTG-KSSWSQNEGWASDRSYINRFVTYLNEG-KFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRW 3026
            S +P  + +G RD    +TK  Q     L  + S   +  +P L  +    R  R +++    LL  P    + A G       +PG      +R    ++ +++  K+  +    GLY PP  ++   L   +  +   + L  H   +Y      + P   RS+ +   EM   +      +GGLL   +   +   K+ +   G  + P F   ++ + ++W   +R   APW+ LA DS +  V     R LA P +++ L+D M     +L      S R E I  D QI  G+MHAGYPI  M+   + P      L +   M  EG WG LHE+GHN Q  ++ F   T + TNN+F +   E + G   + +  +   ++R    R   +++ G     W    F  L  Y QL+  FGW+ + ++F  Y   A     M    K+ +W    S     NL P +  W W
Sbjct:  417 STAPISLRLGLRDFACHLTKKSQLSGPRLTNLVSLLRRDLVPLLGSRDPAFRPLRAIVW---DLLRGPLWPDLAAVGPESLGDIFPGRPLEPTYRGW-QEVTIDVSSKEGLTWVSTGLYAPPAQRVLLDLPSLALGRGIQIQLGCHTDQLYDSKTMKRHPEVTRSMPVVRGEMDRGAMEVASSWGGLLYLLVPEGVSLGKIRVRVRGAARAPYFKLGET-SLQHWQEFERHYPAPWSELACDSLILTVPSEAARGLADPERLMRLWDRMTGAIAYLAAEPHPSARPERIVVDEQISHGWMHAGYPI--MVHRASIPK-----LLNEQEMRAEGMWGPLHELGHNRQRPAWNFPPHTTEATNNLFAVYVHEEVLGVARARAHPDLRPAERE--RRLRDHVDAGCPLAAWTV--FTCLETYLQLQEAFGWDPFVRLFGDYQRAASTPSTM--EAKMSEWAERFSRCVGRNLAPFFSAWGW 876          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003778.1 (pep scaffold:Pmarinus_7.0:GL482904:4425:14035:-1 gene:ENSPMAG00000003419.1 transcript:ENSPMAT00000003778.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 100.138 bits (248), Expect = 8.061e-22
Identity = 60/189 (31.75%), Postives = 88/189 (46.56%), Query Frame = 3
Query:  117 ITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNW--------------------CKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFP----EMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCE 611
            +T +G +Y+GNV  T SG++C  WT     ++  +     + + V   NFCRNP+    GPWC T +  +  E C +P C KD N                     C   A G++Y G +N+T SG+ C+ WS Q    +   P       L+ A   CR+PDG  +G WCY  D  +  D C +  C+
Sbjct:  108 MTGNGEQYIGNVSFTKSGRQCQLWTSNFPHQVRFKPVDHPEKQLVE--NFCRNPDASQDGPWCYTRDPLVPREPCSVPTCDKDPNQHVPVSPRVTPTKPAPRPQGPCVPGA-GEDYRGPVNVTRSGRHCQAWSAQTPHVSRYSPLTHRAAGLEGAA--CRNPDGDEEGAWCYTTDPGLTIDYCALHYCD 291          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: hgfb (hepatocyte growth factor b [Source:ZFIN;Acc:ZDB-GENE-041014-3])

HSP 1 Score: 79.337 bits (194), Expect = 1.626e-15
Identity = 53/195 (27.18%), Postives = 88/195 (45.13%), Query Frame = 3
Query:  117 ITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDI-PFCVK--------DNNWCKDTAEGQEYFGSLNLTESGKDCKLWS---------LQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNC-YVPQCEISFSQIEYFGG 644
            +   G  Y G V+ T S ++C +W ++     N     F +       N+CRNPN   + PWC T +    WE+C I P C          +   C +   G++Y G  N T+ G  C+ W+         L++ +P +G          NYCR+PDG  + PWC+  D +++   C ++P+C    +  + + G
Sbjct:  135 VECRGEHYRGLVDHTDSQRECQRWDLQHPHNHNYTPDRFPNAGLDD--NYCRNPNGRMR-PWCFTTDPHQPWEYCSIKPGCCASKATRPAMEGRPCLEE-RGEDYRGRANHTKGGIPCQHWAAQQPHRHTYLEHNYPCSGL-------DRNYCRNPDGREEAPWCFTLDPSVRRQLCSHIPRCAQPTAGSQCYSG 318          

HSP 2 Score: 78.1814 bits (191), Expect = 4.240e-15
Identity = 60/193 (31.09%), Postives = 88/193 (45.60%), Query Frame = 3
Query:  102 IKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF--LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNK----FPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYV-PQCEISFSQIEYFGGYDFLK 659
            ++ C  T +GS Y G+   T SG  C  W              +   DLE     N CRNP   P  PWC T +   + E C IP C  D   C +   G+ Y G ++ T+S ++C+ W LQ+     +  + FP   L   +NYCR+P+G  + PWC+  D +  W+ C + P C  S +      G   L+
Sbjct:   48 VRGC-YTGNGSGYRGDKNYTESGLPCQAWGDSEPHDSRYLPSFYPAFDLEG----NSCRNPAQEPGEPWCYTMDSNRRRERCGIPRC--DEVPCVE-CRGEHYRGLVDHTDSQRECQRWDLQHPHNHNYTPDRFPNAGLD--DNYCRNPNGRMR-PWCFTTDPHQPWEYCSIKPGCCASKATRPAMEGRPCLE 229          

HSP 3 Score: 77.7962 bits (190), Expect = 5.235e-15
Identity = 55/200 (27.50%), Postives = 86/200 (43.00%), Query Frame = 3
Query:   63 AIFPNLSATYFT----EIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHF----LDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCD-IPFCVKDNNWCKD-TAEGQEYFGSLNLTESGKDCKLWSLQNK----FPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISF 620
            +I P   A+  T    E + C +   G +Y G    T  G  C  W  +   R    E ++    LD       N+CRNP+   + PWC T + +++ + C  IP C +     +  +  G  Y G +  T  G  C+ W+  +     +     P   L+   + CR+PDG   GPWC+  D ++ WD C V  C  +F
Sbjct:  207 SIKPGCCASKATRPAMEGRPC-LEERGEDYRGRANHTKGGIPCQHWAAQQPHRHTYLEHNYPCSGLDR------NYCRNPDGREEAPWCFTLDPSVRRQLCSHIPRCAQPTAGSQCYSGSGTAYRGGVATTRHGLQCQAWNATSPHKPWYTPKSHPGAGLEG--HSCRNPDGDPWGPWCFTSDPHLPWDYCSVEACGDNF 397          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001728.1 (pep scaffold:Pmarinus_7.0:GL477419:32233:56220:-1 gene:ENSPMAG00000001561.1 transcript:ENSPMAT00000001728.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 4.444e-11
Identity = 32/84 (38.10%), Postives = 43/84 (51.19%), Query Frame = 3
Query:  384 TAEGQEYFGSLNLTESGKDCKLWSLQ----NKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFS 623
            T  G  Y G   LT SG  C+ W+ +    + +   GFPE  L+N  NYCR+PD    GPWC   D N+ W+ C + +C    S
Sbjct:   13 TGSGTGYRGLQTLTASGHQCQTWASKTPHLHAYTPEGFPEAGLEN--NYCRNPDSDPNGPWCITQDPNVLWEYCALQRCASGPS 94          

HSP 2 Score: 62.7734 bits (151), Expect = 1.069e-10
Identity = 43/133 (32.33%), Postives = 63/133 (47.37%), Query Frame = 3
Query:  288 PKGPWCVTDEKALQ---WEHCDIPFC---VKDNNWCKDTAE--------GQE-------YFGSLNLTESGKDCKLWSLQNKF-----PANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQC 608
            P G WC +     +   WEHCDI +C   ++ N + +  A+        GQ+       Y G+  +T  GK C+ W+ Q+       PAN + +  L  +  YCR+PDG   GPWCY  DS  +W  C +  C
Sbjct:  157 PPGLWCNSANNHFRRNSWEHCDI-YCDSFIQTNPFARPNADTLPSTALFGQDCVFGSRGYRGAQAVTSGGKTCQAWASQSPHRHQFVPAN-YTDADL--SLGYCRNPDGDLAGPWCYTTDSATRWQYCGIAHC 285          

HSP 3 Score: 54.299 bits (129), Expect = 5.054e-8
Identity = 35/122 (28.69%), Postives = 51/122 (41.80%), Query Frame = 3
Query:   15 YFKRN------LYFQIFLLLNYAIFPNLSATYFTEI--KKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFC 356
            +F+RN      +Y   F+  N    PN      T +  + C   S G  Y G    T  GK C  W  +   R      ++ D +      +CRNP+    GPWC T + A +W++C I  C
Sbjct:  168 HFRRNSWEHCDIYCDSFIQTNPFARPNADTLPSTALFGQDCVFGSRG--YRGAQAVTSGGKTCQAWASQSPHRHQFVPANYTDADLSL--GYCRNPDGDLAGPWCYTTDSATRWQYCGIAHC 285          

HSP 4 Score: 51.2174 bits (121), Expect = 5.898e-7
Identity = 26/81 (32.10%), Postives = 39/81 (48.15%), Query Frame = 3
Query:  120 TSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVK 362
            T  G+ Y G    T SG +C  W  +         + F   E+   +N+CRNP+  P GPWC+T +  + WE+C +  C  
Sbjct:   13 TGSGTGYRGLQTLTASGHQCQTWASKTPHLHAYTPEGFP--EAGLENNYCRNPDSDPNGPWCITQDPNVLWEYCALQRCAS 91          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005055.1 (pep scaffold:Pmarinus_7.0:GL479085:195887:200886:-1 gene:ENSPMAG00000004594.1 transcript:ENSPMAT00000005055.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 8.947e-9
Identity = 32/99 (32.32%), Postives = 41/99 (41.41%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNL----------EEKHFLDLESVSPH-------------NFCRNPNLYPKGPWCVTDEKALQWEHCDIPFC 356
            G  Y G V KT SG++C  W+     R               +H   + S S H             NFCRNP+    GPWC T + A +WE C +P C
Sbjct:   47 GISYRGTVSKTTSGRQCQFWSTNKPHRPKYLPFSRCEWTNARQHLSVMLSASHHFTPWTHPELGLEDNFCRNPDHGVSGPWCYTTDPAQRWEVCSVPAC 145          

HSP 2 Score: 52.7582 bits (125), Expect = 3.637e-8
Identity = 29/102 (28.43%), Postives = 42/102 (41.18%), Query Frame = 3
Query:  393 GQEYFGSLNLTESGKDCKLWS-----------------------------LQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCE 611
            G  Y G+++ T SG+ C+ WS                               + F     PE+ L+  +N+CR+PD    GPWCY  D   +W+ C VP C 
Sbjct:   47 GISYRGTVSKTTSGRQCQFWSTNKPHRPKYLPFSRCEWTNARQHLSVMLSASHHFTPWTHPELGLE--DNFCRNPDHGVSGPWCYTTDPAQRWEVCSVPACR 146          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Match: EDO39763 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S935])

HSP 1 Score: 65.855 bits (159), Expect = 4.322e-13
Identity = 31/77 (40.26%), Postives = 45/77 (58.44%), Query Frame = 3
Query:  387 AEGQEYFGSLNLTESGKDCKLWSLQ--NKFPANGFPEMSLQNAENYCRSPDGYS-KGPWCYVDDSNIKWDNCYVPQC 608
             EG  Y G++N+T+SG  C+ WS Q  ++   N      L NA N+CR+P G    GPWCY  +  ++W+ C VP+C
Sbjct:   14 GEGVGYHGNINVTKSGISCQPWSSQCPHRHYHNSTQYTELINASNFCRNPGGMGPDGPWCYTTNMMVRWEYCDVPKC 90          

HSP 2 Score: 61.6178 bits (148), Expect = 1.481e-11
Identity = 29/77 (37.66%), Postives = 41/77 (53.25%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPN-LYPKGPWCVTDEKALQWEHCDIPFC 356
            G  Y GN+  T SG  C  W+ +   R       + +L  ++  NFCRNP  + P GPWC T    ++WE+CD+P C
Sbjct:   16 GVGYHGNINVTKSGISCQPWSSQCPHRHYHNSTQYTEL--INASNFCRNPGGMGPDGPWCYTTNMMVRWEYCDVPKC 90          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Match: EDO28926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T536])

HSP 1 Score: 60.4622 bits (145), Expect = 2.321e-11
Identity = 30/77 (38.96%), Postives = 41/77 (53.25%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPN-LYPKGPWCVTDEKALQWEHCDIPFC 356
            G  Y GN+  T SG  C  W+ +           + +L + S  NFCRNP  + P GPWC T  K ++WE+CD+P C
Sbjct:    7 GVGYHGNINVTKSGISCQPWSSQCPHHHYHNSTQYPELINAS--NFCRNPGGMGPDGPWCYTTNKLVRWEYCDVPKC 81          

HSP 2 Score: 60.077 bits (144), Expect = 2.461e-11
Identity = 32/79 (40.51%), Postives = 47/79 (59.49%), Query Frame = 3
Query:  387 AEGQEYFGSLNLTESGKDCKLWSLQ----NKFPANGFPEMSLQNAENYCRSPDGYSK-GPWCYVDDSNIKWDNCYVPQC 608
             EG  Y G++N+T+SG  C+ WS Q    +   +  +PE  L NA N+CR+P G    GPWCY  +  ++W+ C VP+C
Sbjct:    5 GEGVGYHGNINVTKSGISCQPWSSQCPHHHYHNSTQYPE--LINASNFCRNPGGMGPDGPWCYTTNKLVRWEYCDVPKC 81          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Match: EDO30193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1H4])

HSP 1 Score: 51.2174 bits (121), Expect = 4.976e-8
Identity = 24/84 (28.57%), Postives = 41/84 (48.81%), Query Frame = 3
Query:  390 EGQEYFGSLNLTESGKDCKLWSLQ--NKFPANGFPEMSLQNA---ENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQ 626
            +G++Y G++N T  G  C+ W+ Q  ++      P ++ +      NYCR+P G    PWCY      +W  C +  C  + S+
Sbjct:    1 DGRDYRGTMNFTAKGVTCQPWNEQWPHQHTEMADPLVAAREGLGKHNYCRNPGGRKHRPWCYTTLEKTRWQYCEIRSCRKTKSK 84          

HSP 2 Score: 47.3654 bits (111), Expect = 1.112e-6
Identity = 23/79 (29.11%), Postives = 36/79 (45.57%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIEL-LRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVK 362
            G +Y G +  T  G  C  W  +   +   + +      E +  HN+CRNP      PWC T  +  +W++C+I  C K
Sbjct:    2 GRDYRGTMNFTAKGVTCQPWNEQWPHQHTEMADPLVAAREGLGKHNYCRNPGGRKHRPWCYTTLEKTRWQYCEIRSCRK 80          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Match: EDO43993 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RX97])

HSP 1 Score: 53.9138 bits (128), Expect = 3.169e-7
Identity = 29/90 (32.22%), Postives = 47/90 (52.22%), Query Frame = 3
Query:  360 KDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQI 629
            +DN +C +   G++Y G LN+T+ G  C  W   +       P   L  + N+CR+P G  + PWCYVD +  ++  C +P+C  +   I
Sbjct:  127 EDNQYCYN-GTGEDYIGRLNVTKYGMPCLSWKSND-------PVYKL--SHNFCRNPRGQHEAPWCYVDKNTYEY--CVIPKCSEASDAI 204          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Match: EDO48215 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKA9])

HSP 1 Score: 53.9138 bits (128), Expect = 3.524e-7
Identity = 23/75 (30.67%), Postives = 40/75 (53.33%), Query Frame = 3
Query:  393 GQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEM--SLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCE 611
            G+ Y G++++TE G  C+ W++         P     L+   NYCR+P G  + PWC+  D   ++D C + +C+
Sbjct:  258 GEHYNGNISVTEKGFGCQRWNVTTPHFHLMSPATYHELRGGHNYCRNPGGKKQRPWCFTTDPRQRYDYCDIAKCD 332          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: si:ch211-210b2.3 (TRPM8 channel-associated factor homolog [Source:NCBI gene;Acc:101159605])

HSP 1 Score: 118.242 bits (295), Expect = 1.855e-26
Identity = 89/340 (26.18%), Postives = 158/340 (46.47%), Query Frame = 3
Query: 2067 DIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDND--KVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTF-TGTVDVTNNIFTLVGVESLTG--KSSWSQNEGWASDRSYINRFVTYLNEGK-FDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYP 3068
            D    +E  + P       ++   MQ ++  +GGL+ +      D   V+++    +  P + +      + W++L+ + +PWA L  D+ +  V    VR L +P ++ +L++ ++K    L     +  R+E    D+QI  G+MHAGYPI      V         L +V +M   G WG +HE+GHN Q   + F   T + T N++++   E + G  ++    N    + +S   R   Y+ +G+  + W  E ++AL  Y QL+  FGW+ + +VF  Y   + F     +  K+  +    S    M+L   +  W WPI ++T Q+L   P
Sbjct:  582 DQLNAEELKRPPRVHEQFAVTSEMMQVWNL-WGGLIYLVAPPQADVHGVEVIVQMAVPAPYY-KSGVTTAEAWSLLRTAPSPWAELEFDNIILTVPSEAVRTLERPEELAQLWNKIMKAIADLAAKPEKFPRKERFVADVQISHGWMHAGYPIMGQKSAVNE-------LVNVDHMKSCGLWGPIHELGHNQQRGCWEFPPNTTECTCNLWSVYVHEEVLGIDRAKAHPNMSLENRKS---RARNYVTQGRNLNSW--EMWVALETYMQLQDKFGWDAFKKVFAAYLQMSQFPGD--NPGKMNLYTETFSQTVGMDLTAFFKAWGWPIQASTEQKLSNLP 905          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: plat (plasminogen activator, tissue type [Source:NCBI gene;Acc:100049466])

HSP 1 Score: 88.9669 bits (219), Expect = 8.696e-18
Identity = 62/189 (32.80%), Postives = 87/189 (46.03%), Query Frame = 3
Query:  105 KKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNL-EEKHFLDLESVSPHNFCRNPNLYPKGPWC-VTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSL-------QNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYV-DDSNIKWDNCYVPQCEISFSQIEYFG 641
            +KC I   G  Y G +  T SG++CI W    LR       K       +  HNFCRNP+     PWC +     + WE C +P C  D N       G+ Y G+  +T+SG  C  W          N + +N   ++ L N  N+CR+PDG S GPWC+      + W+ C +P+C  + S I   G
Sbjct:   76 EKC-IVGKGEGYRGTLSVTHSGEECINWNSTSLRGKKFTARKTDASGLGLGNHNFCRNPD-QDSAPWCYIYKGTQVIWELCSLPKCSGDRNRECVQGLGKSYRGTAAVTKSGSACLPWDAPAVRRKRNNAWKSNAL-DLGLSN-HNFCRNPDGDS-GPWCHTYKKMQLTWELCDIPKCSRAPSTITTLG 259          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: plat (plasminogen activator, tissue type [Source:NCBI gene;Acc:100049466])

HSP 1 Score: 88.9669 bits (219), Expect = 1.199e-17
Identity = 62/189 (32.80%), Postives = 87/189 (46.03%), Query Frame = 3
Query:  105 KKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNL-EEKHFLDLESVSPHNFCRNPNLYPKGPWC-VTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSL-------QNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYV-DDSNIKWDNCYVPQCEISFSQIEYFG 641
            +KC I   G  Y G +  T SG++CI W    LR       K       +  HNFCRNP+     PWC +     + WE C +P C  D N       G+ Y G+  +T+SG  C  W          N + +N   ++ L N  N+CR+PDG S GPWC+      + W+ C +P+C  + S I   G
Sbjct:  108 EKC-IVGKGEGYRGTLSVTHSGEECINWNSTSLRGKKFTARKTDASGLGLGNHNFCRNPD-QDSAPWCYIYKGTQVIWELCSLPKCSGDRNRECVQGLGKSYRGTAAVTKSGSACLPWDAPAVRRKRNNAWKSNAL-DLGLSN-HNFCRNPDGDS-GPWCHTYKKMQLTWELCDIPKCSRAPSTITTLG 291          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: plat (plasminogen activator, tissue type [Source:NCBI gene;Acc:100049466])

HSP 1 Score: 88.9669 bits (219), Expect = 1.262e-17
Identity = 62/189 (32.80%), Postives = 87/189 (46.03%), Query Frame = 3
Query:  105 KKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNL-EEKHFLDLESVSPHNFCRNPNLYPKGPWC-VTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSL-------QNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYV-DDSNIKWDNCYVPQCEISFSQIEYFG 641
            +KC I   G  Y G +  T SG++CI W    LR       K       +  HNFCRNP+     PWC +     + WE C +P C  D N       G+ Y G+  +T+SG  C  W          N + +N   ++ L N  N+CR+PDG S GPWC+      + W+ C +P+C  + S I   G
Sbjct:  148 EKC-IVGKGEGYRGTLSVTHSGEECINWNSTSLRGKKFTARKTDASGLGLGNHNFCRNPD-QDSAPWCYIYKGTQVIWELCSLPKCSGDRNRECVQGLGKSYRGTAAVTKSGSACLPWDAPAVRRKRNNAWKSNAL-DLGLSN-HNFCRNPDGDS-GPWCHTYKKMQLTWELCDIPKCSRAPSTITTLG 331          
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Match: hgfb (hepatocyte growth factor [Source:NCBI gene;Acc:101167035])

HSP 1 Score: 85.1149 bits (209), Expect = 2.344e-16
Identity = 65/207 (31.40%), Postives = 97/207 (46.86%), Query Frame = 3
Query:   18 FKRNL-YFQIFLLLNYAIFPNLSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWT--IELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEH--CDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSL----QNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCE 611
            F RN    QI+   NY ++          +++C I   G  Y G    T +G  C  W   I    +   ++    DL      N+CRNP+    GPWC T +   Q  H  C IP C +    C +   G++Y G ++ TESGK+C+ W L    ++ F    +P+  L   +NYCR+P+G  + PWC+  D N  W+ C +  CE
Sbjct:   85 FDRNAPGAQIYQNPNYELYQKKDY-----VREC-ILGTGQSYRGRRSVTVNGILCQAWASPIPHEHKFMSKKFKKSDLRE----NYCRNPDNSTVGPWCFTTDPRPQLRHQECGIPQCSEVE--CIN-CNGEDYRGPMDHTESGKECQRWDLDEPHKHLFHPKRYPDKGLD--DNYCRNPNGRHR-PWCFTTDPNTPWEYCTIKVCE 275          

HSP 2 Score: 80.1073 bits (196), Expect = 8.094e-15
Identity = 56/176 (31.82%), Postives = 79/176 (44.89%), Query Frame = 3
Query:  129 GSEYVGNVEKTFSGKKCIKWTIELLRRLNL--EEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHC-DIPFCVKDNNWCKDTAE--GQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFG 641
            G  Y G V+ T +G  C +W  +     +   E     DL      N+CRNP+     PWC T +  ++   C +IP C   N    +  E  G+ Y G  + T S   C  W   +     G   + L+   NYCR+PDG   GPWCY ++S I WD C +  C+ S + I   G
Sbjct:  293 GEGYRGVVDMTPTGLTCQRWDSQYPHNHSFIPEAYRCKDLRE----NYCRNPDGQ-DFPWCFTTDPRVRTMFCTNIPQCGTQNRLANECYESLGENYRGQQSRTRSNLPCGPWREHSNSGERGMLMVGLEG--NYCRNPDGDKHGPWCYTNNSAISWDYCSIKPCDASQNTIPQDG 461          
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000026283.1 (SMESG000026283.1)

HSP 1 Score: 2214.11 bits (5736), Expect = 0.000e+0
Identity = 1069/1077 (99.26%), Postives = 1072/1077 (99.54%), Query Frame = 3
Query:    9 MFYFKRNLYFQIFLLLNYAIFPNLSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFIVVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFISLLMKTEYKIEYRLGENYEIKNVLSSNLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLILKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTDYVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIEK 3239
            MFYFKRNLYFQIFLLLNYAIFPNLSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIP CVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPAN FPEMSLQNAEN+CRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITF+VVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFIS LMKTEYKIEYRLGENYEIKNVLS NLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLI KDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTDYVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLN+VSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIEK
Sbjct:    1 MFYFKRNLYFQIFLLLNYAIFPNLSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPLCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANAFPEMSLQNAENHCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFVVVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFISPLMKTEYKIEYRLGENYEIKNVLSLNLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLIFKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTDYVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNSVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIEK 1077          
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000026302.1 (SMESG000026302.1)

HSP 1 Score: 1222.22 bits (3161), Expect = 0.000e+0
Identity = 580/1052 (55.13%), Postives = 766/1052 (72.81%), Query Frame = 3
Query:   84 ATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFIVVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFISLLMKTEYKIEYRLGENYEIKNVLSSNLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLILKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTDYVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIEK 3239
            ATYF EI KCK+TSHGSEY+G V KT SGK C KWT +L++   L E++ LDL  VSPHN+CRN NL PKGPWC T E+ L+WE+C+IP C K+NNWCKD+  G+EY G +N+T SG+ C LW+ +N +  +  PE S + A+NYCRS   + + P C +      W+ C+V  CE   +Q EYFGG DFLK +   LN+I+  N+ K  +  + I++ KW+L  +S+  LW T YDP CQ+D +EYE+C +S+S + ++ + L Y CN+P+KNLIFIS L+KT+YK++Y L E YE+K  L+ +  +YQ+K+DKYL   +     +P C NFGSFL++  N+FPYAIT +N +VF ISEI KGR+G IACD IT +Y  FK +L+TIY  N+++PV+L   +IK L ++S+    +  Q IWNAH  I   F  KL++LIK GL+IV+G+KP AY+ +  NL +SEL TLFK++G+WP++ ++N  +    +D I+ +PL    ++AKTNL NL  VS SI NI+   PFQ +G+ ++F ++ +NI+ELI IYS+ +KS IPC+ +   E++ERNLIYFY+ LLIYPFN  +VAPGVSFYPGN  SDN++ L VQ+ LNLKFK++  QLGLYLPPGK+G A    S SQTC+LYLNFHL D+++ + Y KWPV+ +   L I E+Q+FSFPFGGLLS+SC  +NDKVD++  GVIQVPVFDRRDS+NNK W  LQ+SK PWAVLAGDSFVFVVSIN VRNL  P KVL+L+D MVKE YHLRG ++++ RR LISTDIQI   + + GYPIGT I++VTNP  +S++LFD  N+ + G W VL+EIG NL+ S        D  +N+F LVG E +T KSSWS+NEGWAS+ +YIN+ V+Y+N GKF + +++P LALG+YAQLR+NF WEL+YQVFR+YN     +++M D EKIQ WI +TS  AKMNLCPLYD WRWPID  TNQEL KY +F PDDFL KLV  +V KI  KYGGSL VKR+  KLEE++GPLKNK  ++GERIIEK
Sbjct:   18 ATYFEEIAKCKLTSHGSEYIGTVNKTLSGKLCTKWTADLVKNFKLNERNLLDLNMVSPHNYCRNYNLDPKGPWCATGEETLKWEYCNIPSCKKENNWCKDSIIGREYLGIINVTISGEKCLLWTPRNWYKESNLPEKSRELAKNYCRSLGNHKEEPVCIIAKQPF-WEKCHVTLCEKELTQSEYFGGSDFLKHMAGELNIIRFPNVYKYASIKISIEIKKWTLNNKSKMALWGTEYDPLCQRDLDEYEYCEISRSIRTESHMHLFYSCNRPLKNLIFISPLLKTDYKVDYNLEEMYEVKMQLNKDFMKYQKKMDKYLNAKKNIPSLLPYCPNFGSFLLISKNSFPYAITRNNKIVFAISEIEKGRVGFIACDNITENYESFKGFLQTIYEVNEIHPVVLNQSLIKSLSEESLNDTIIKRQFIWNAH-RIVGDFSAKLLELIKNGLRIVIGIKPSAYDTFDGNLVNSELQTLFKSAGIWPTMDSLNETIIKTKKDIISVYPLIQQFDYAKTNLTNLKDVSESISNIIVKVPFQRIGNENDFIDLNENIEELINIYSNQIKSLIPCMGRPALEKSERNLIYFYNTLLIYPFNPPIVAPGVSFYPGNFLSDNYKPLKVQLTLNLKFKNDPVQLGLYLPPGKLGNAKILTSNSQTCILYLNFHLSDVHENNRYDKWPVTMKKFWLQIVEVQSFSFPFGGLLSMSCDSNNDKVDIILYGVIQVPVFDRRDSENNKKWITLQKSKVPWAVLAGDSFVFVVSINAVRNLGDPDKVLQLYDSMVKEGYHLRGKSIKAYRRALISTDIQITGKHKNTGYPIGTSIKYVTNPKSQSQYLFDFNNIKLNGNWFVLYEIGLNLRSSPLYIISEKDAISNLFALVGTEYMTNKSSWSKNEGWASNATYINQTVSYINNGKFSDLQSKPSLALGIYAQLRANFQWELFYQVFREYNKIEKSVEKMSDKEKIQHWIKMTSHKAKMNLCPLYDLWRWPIDKETNQELSKYSFFLPDDFLMKLVPERVVKIQSKYGGSLKVKRKGKKLEEIVGPLKNKLKIEGERIIEK 1067          
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000026300.1 (SMESG000026300.1)

HSP 1 Score: 993.03 bits (2566), Expect = 0.000e+0
Identity = 466/1054 (44.21%), Postives = 692/1054 (65.65%), Query Frame = 3
Query:   81 SATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFIVVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFISLLMKTEYKIEYRLGENYEIKNVLSSNLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLILKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTD--YVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIE 3236
            S+ YF EI+KCKIT+HGSEY+G+V++T +GK C KW+ E +++  L+   + D ES+  HNFCRNPN  P GPWC+ D +  +WEHCDIP C+KD NWCKD+  G EYFG++N T+SGK C++WS ++ F    FPE +L++  N CR+PDG + GPWC+V   +  W+NC +P C+   SQIEYFGGYDF K ++D   VI LK +  +I F V ++ T W+   Q +T+LWAT Y+P CQ D NEYEFC  ++ +    ++ ++Y+C +P+K LIFI  L  + YK E+ +G+ YEI    +    +Y++K+ +++       +++P C N G F +L DN  PYA+ SD+ V  +ISE+GKGRIG + C+T   +      KD+L ++YG++  +P +LT +++  L+ +          +IWNAH  +  S  K L+DL+K GLKI++ + PWAY     +L   EL ++F  +G+WPS    +  + ++ R ++  FPL HL  +AKT++ +   VS SI  I+  +PF  + S  +  +I + +  LI IYS  + S IPCL K +  + ++  I+F++ LL++   G + APGVSFYPG +  D  + + V++ L+++F+ E   LGLYLPPGK  +     + S  C LY+N HLFD+ +   + KWP+ K+S+ +   +++ FSF FGGLLS+SC   + KV +V +G I +P++DRR    NK+W     +  PWA+++ D  VF+V    +RNLA P  VLEL+D MV EE++L+G ++++  RE++S+D+QI AGYM AGYPIG ++  VTNP+ K +FLF++  +   GRWG+LHEIGHNLQ+S FTF+GT +VT NIFTLVG+E+ TG+ SWS N  W      ++  ++Y+  G F +WK +PFLAL  YAQL+S FGWE Y Q+FR+Y+     +  + D+EKIQ WI  TS   +MNLCP YD+W+WPI+  T + L K P FFPDDFLTK++  KVD+II KYGGS ++KRQP  + ++IGPLK+K S+ G+ +I+
Sbjct:   30 SSKYFAEIQKCKITTHGSEYIGSVDRTKTGKICQKWSEENIKKFKLQFLDYPDRESIVTHNFCRNPNQVPDGPWCIVDRENSKWEHCDIPSCIKDRNWCKDSELGGEYFGNINFTKSGKPCRVWSDKDTFDGKSFPEKNLKDVGNKCRNPDGKTSGPWCFVSSEDDNWENCNIPNCDEDLSQIEYFGGYDFQKAVEDQKTVIPLKMVYSSIKFKVFLNKTIWTPDVQRKTSLWATDYNPSCQTDLNEYEFCKSTEQSVNDERVEIVYRCLRPLKYLIFIFPLESSLYKTEHHVGKTYEINEKPNLQFVKYEKKLLQHISSKNRKNLTIPVCDNPGYFYLLNDNVIPYAVMSDSRVFAMISEVGKGRIGFMGCNTYLEESAQAEIKDFLNSVYGDDFTSPKVLTKELLDLLNSERNNIETSLPTVIWNAHNSLGFSTTKVLLDLVKDGLKIIILMTPWAYPSLNSSLSQIELSSIFLEAGIWPSRDISSDPISVLGRISVHTFPLIHLFNYAKTSISSSMKVSKSITTIILNTPFDSLMSNVQMEKIKEALHSLILIYSPLINSLIPCLKKPLRSKNDKFKIFFFNILLLHDLYGNVSAPGVSFYPGKVYVDEVKPVKVKIKLSMEFEKEVIPLGLYLPPGKTVSVNITQAVSSCCQLYINHHLFDVSENSLWKKWPIIKKSLQIEKFKIKQFSFSFGGLLSLSCEKSDKKVHVVVNGAIPIPMYDRRGGSINKHWKQSYFTNVPWAIISSDYVVFIVPFVAIRNLAYPPSVLELYDAMVAEEFYLKGKSIENSTREIVSSDVQIVAGYMIAGYPIGVLMYAVTNPASKVEFLFNLEYIKSNGRWGILHEIGHNLQESIFTFSGTNEVTTNIFTLVGIETFTGQDSWSANMSWFP----MSSVISYIKSGSFSDWKIDPFLALCTYAQLKSQFGWEFYSQLFRRYDLLNKKLGELTDSEKIQLWITETSNRVRMNLCPFYDWWKWPIEDTTKESLSKLPIFFPDDFLTKMLPEKVDEIIEKYGGSSLMKRQPLNILQVIGPLKHKRSIRGKSVID 1079          
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000026300.1 (SMESG000026300.1)

HSP 1 Score: 992.26 bits (2564), Expect = 0.000e+0
Identity = 466/1054 (44.21%), Postives = 692/1054 (65.65%), Query Frame = 3
Query:   81 SATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFIVVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFISLLMKTEYKIEYRLGENYEIKNVLSSNLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLILKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTD--YVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIE 3236
            S+ YF EI+KCKIT+HGSEY+G+V++T +GK C KW+ E +++  L+   + D ES+  HNFCRNPN  P GPWC+ D +  +WEHCDIP C+KD NWCKD+  G EYFG++N T+SGK C++WS ++ F    FPE +L++  N CR+PDG + GPWC+V   +  W+NC +P C+   SQIEYFGGYDF K ++D   VI LK +  +I F V ++ T W+   Q +T+LWAT Y+P CQ D NEYEFC  ++ +    ++ ++Y+C +P+K LIFI  L  + YK E+ +G+ YEI    +    +Y++K+ +++       +++P C N G F +L DN  PYA+ SD+ V  +ISE+GKGRIG + C+T   +      KD+L ++YG++  +P +LT +++  L+ +          +IWNAH  +  S  K L+DL+K GLKI++ + PWAY     +L   EL ++F  +G+WPS    +  + ++ R ++  FPL HL  +AKT++ +   VS SI  I+  +PF  + S  +  +I + +  LI IYS  + S IPCL K +  + ++  I+F++ LL++   G + APGVSFYPG +  D  + + V++ L+++F+ E   LGLYLPPGK  +     + S  C LY+N HLFD+ +   + KWP+ K+S+ +   +++ FSF FGGLLS+SC   + KV +V +G I +P++DRR    NK+W     +  PWA+++ D  VF+V    +RNLA P  VLEL+D MV EE++L+G ++++  RE++S+D+QI AGYM AGYPIG ++  VTNP+ K +FLF++  +   GRWG+LHEIGHNLQ+S FTF+GT +VT NIFTLVG+E+ TG+ SWS N  W      ++  ++Y+  G F +WK +PFLAL  YAQL+S FGWE Y Q+FR+Y+     +  + D+EKIQ WI  TS   +MNLCP YD+W+WPI+  T + L K P FFPDDFLTK++  KVD+II KYGGS ++KRQP  + ++IGPLK+K S+ G+ +I+
Sbjct:    8 SSKYFAEIQKCKITTHGSEYIGSVDRTKTGKICQKWSEENIKKFKLQFLDYPDRESIVTHNFCRNPNQVPDGPWCIVDRENSKWEHCDIPSCIKDRNWCKDSELGGEYFGNINFTKSGKPCRVWSDKDTFDGKSFPEKNLKDVGNKCRNPDGKTSGPWCFVSSEDDNWENCNIPNCDEDLSQIEYFGGYDFQKAVEDQKTVIPLKMVYSSIKFKVFLNKTIWTPDVQRKTSLWATDYNPSCQTDLNEYEFCKSTEQSVNDERVEIVYRCLRPLKYLIFIFPLESSLYKTEHHVGKTYEINEKPNLQFVKYEKKLLQHISSKNRKNLTIPVCDNPGYFYLLNDNVIPYAVMSDSRVFAMISEVGKGRIGFMGCNTYLEESAQAEIKDFLNSVYGDDFTSPKVLTKELLDLLNSERNNIETSLPTVIWNAHNSLGFSTTKVLLDLVKDGLKIIILMTPWAYPSLNSSLSQIELSSIFLEAGIWPSRDISSDPISVLGRISVHTFPLIHLFNYAKTSISSSMKVSKSITTIILNTPFDSLMSNVQMEKIKEALHSLILIYSPLINSLIPCLKKPLRSKNDKFKIFFFNILLLHDLYGNVSAPGVSFYPGKVYVDEVKPVKVKIKLSMEFEKEVIPLGLYLPPGKTVSVNITQAVSSCCQLYINHHLFDVSENSLWKKWPIIKKSLQIEKFKIKQFSFSFGGLLSLSCEKSDKKVHVVVNGAIPIPMYDRRGGSINKHWKQSYFTNVPWAIISSDYVVFIVPFVAIRNLAYPPSVLELYDAMVAEEFYLKGKSIENSTREIVSSDVQIVAGYMIAGYPIGVLMYAVTNPASKVEFLFNLEYIKSNGRWGILHEIGHNLQESIFTFSGTNEVTTNIFTLVGIETFTGQDSWSANMSWFP----MSSVISYIKSGSFSDWKIDPFLALCTYAQLKSQFGWEFYSQLFRRYDLLNKKLGELTDSEKIQLWITETSNRVRMNLCPFYDWWKWPIEDTTKESLSKLPIFFPDDFLTKMLPEKVDEIIEKYGGSSLMKRQPLNILQVIGPLKHKRSIRGKSVID 1057          
BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Match: SMESG000026377.1 (SMESG000026377.1)

HSP 1 Score: 982.245 bits (2538), Expect = 0.000e+0
Identity = 483/1070 (45.14%), Postives = 675/1070 (63.08%), Query Frame = 3
Query:   36 FQIFLLLNYAIFPNLSATYFTEIKKCKITSHGSEYVGNVEKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPWCVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLWSLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVPQCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFIVVIDLTKWSLYAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMKNLIFISLLMKTEYKIEYRLGENYEIKNVLSSNLKEYQEKVDKYLKVHQTNIISMPSCSNFGSFLILKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDTITTD--YVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIWNAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFKASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIGNIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERTERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKFKDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWPVSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDRRDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFDLMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPSYKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVGVESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYAQLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLCPLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLVVKRQPAKLEEMIGPLKNKTSVDGERIIEK 3239
            F IFL     +  +   +YFTEI+ CKIT+HG EYVG    + +G+ C+ W  E L  + +++  + D  S+   N+CRNP  +P GPWC+ DE  L+WEHC I  C+K+ NWCKDT +G EY G LN T++GK CK W +Q+ +  N FPE S Q   N CR+PD  S GPWC+  D + K + C VP+C+  FSQIEYFGGYDFLKT+   +  I L +I +T+T  V  +L   +     +T+LWAT Y+P+CQ D +EYE+C + +S        + Y C +PMK LIFI  + K+EY I+Y LG  Y      S    +Y+ K+++Y +      + +PS SN G F +L DN  PYA++  N V   IS IGKGRI  +A + +     Y   +D  + +Y +   NP LLT    K L D++     M    IWNAH  +    V  L+ LIK GL+I++G+KPWAY YY+     SEL  LF+ +G+WPS  + +   + V + +    P  + LE+AK NL++L  +S SIGN ++  PF  + S+  F  +  +I +L   Y  ++K  IPC   ++T+ ++++LI  ++ +LI  F   ++APG+SFYPG  QS +      QM LNLK+KDE SQLGLY+ PGK  +     S SQ+C +++NFHLF+   K++ YKWP++K   ++  N ++TF++PFGGLLSISC  +NDKV + F  V++VPVFDRRD  + K+W   Q   +PWAV++ D+ VFV+S N +RNLA P   + ++D MVKEE+HL+G ++ +  RE+ISTDIQI AGYMHAGYPIGT I  VTNP  K  + FD  N+ I+G WG++HE+GHNLQ+  FTF G  +V NNIFTLV +ES+T  SSWS N+GW S +  I   V YLN G F+EWK   ++AL +YAQLR++FGWE Y QVFR+YN     I  + D EKIQ WI  TS  + MNLCPLY+ W+WPI   T +EL K   F PDDFLTK++  +VD+II K+GG   V R P  + E IGPLK+K S++G ++I++
Sbjct:    2 FNIFLATALLLQSSFILSYFTEIENCKITTHGREYVGYTNVSKNGRPCLSWKKEYLNDIIMDDSIYFDTTSIIETNYCRNPTNFPNGPWCIVDESILEWEHCHIRSCIKERNWCKDTDKGTEYLGDLNFTKTGKICKPWIIQSNYSENSFPEKSFQLVGNKCRNPD-MSAGPWCFYSD-DYKREECEVPRCDEKFSQIEYFGGYDFLKTISSLITTITLTDICQTVTVTVTFNLESLNSDILRKTSLWATDYNPKCQTDLDEYEYCTIVESKNTSKSYIISYSCFRPMKYLIFICPIDKSEYTIKY-LGTFYNFNLSPSKQFDDYEIKMNEYFQKKIKQDVIVPSDSNHGHFYLLNDNVIPYALSVKNKVTVTISSIGKGRISFLATEKLVNPEFYPELQDLFQEVYEKPVENPKLLTKKYFKALIDENKLQTAMFEHFIWNAHDTLGRDLVFILLQLIKSGLQIIIGVKPWAYYYYQAQFLDSELKVLFETAGIWPSKDSDSDVWRTVTKFSTNTMPFYYRLEYAKKNLMSLKNLSKSIGNFLAEVPFHKIMSKRTFELLKSSIVDLYSNYLPYIKHLIPCSKGSITKESDQDLIKLFNYILISFFGRTLIAPGLSFYPGTFQSSDILLENFQMQLNLKYKDEVSQLGLYVVPGKHVSVQITESNSQSCRIFINFHLFNTINKEKLYKWPITKTEFIIKRNILETFAYPFGGLLSISCDANNDKVKITFINVVKVPVFDRRDKKDTKDWIQQQSVVSPWAVVSSDTLVFVLSSNAIRNLADPETFMRMYDSMVKEEFHLKGRSISTSYREIISTDIQITAGYMHAGYPIGTQIFWVTNPYNKKDYFFDKYNIEIDGEWGLMHELGHNLQEPIFTFNGAGEVNNNIFTLVAIESMTNMSSWSPNDGWVSTKVKIKDLVKYLNNGTFEEWKKTYYVALSIYAQLRNSFGWEFYSQVFRRYNELKNEIGSISDNEKIQLWIKTTSEISGMNLCPLYELWKWPISQKTEKELSKLQMFLPDDFLTKMIEKRVDEIISKFGGIDSVFRTPLTIPEKIGPLKHKFSIEGTKVIQE 1068          
The following BLAST results are available for this feature:
BLAST of Peptidase M60 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TCAF24.598e-3127.03TRPM8 channel associated factor 2 [Source:HGNC Sym... [more]
TCAF24.598e-3127.03TRPM8 channel associated factor 2 [Source:HGNC Sym... [more]
TCAF25.358e-3127.03TRPM8 channel associated factor 2 [Source:HGNC Sym... [more]
TCAF2C7.398e-3126.88TRPM8 channel associated factor 2C [Source:HGNC Sy... [more]
TCAF11.403e-2725.07TRPM8 channel associated factor 1 [Source:HGNC Sym... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cam-13.132e-840.24Tyrosine-protein kinase receptor cam-1 [Source:Un... [more]
cam-13.356e-840.24Tyrosine-protein kinase receptor cam-1 [Source:Un... [more]
cam-13.519e-840.24Tyrosine-protein kinase receptor cam-1 [Source:Un... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nrk3.336e-936.84gene:FBgn0020391 transcript:FBtr0087728[more]
Ror1.083e-637.84gene:FBgn0010407 transcript:FBtr0079978[more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-210b2.35.499e-2926.42si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-... [more]
zgc:1621932.067e-2827.39zgc:162193 [Source:NCBI gene;Acc:323315][more]
zgc:1621932.067e-2827.39zgc:162193 [Source:NCBI gene;Acc:323315][more]
zgc:1621932.067e-2827.39zgc:162193 [Source:NCBI gene;Acc:323315][more]
si:ch211-210b2.41.322e-2726.80si:ch211-210b2.4 [Source:ZFIN;Acc:ZDB-GENE-131122-... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000045101.13.145e-3628.35TRPM8 channel-associated factor 2 [Source:NCBI gen... [more]
ENSXETT00000002244.13.463e-3427.88TRPM8 channel-associated factor homolog [Source:NC... [more]
ENSXETT00000045059.11.169e-3127.01TRPM8 channel-associated factor 2 [Source:NCBI gen... [more]
ENSXETT00000045084.11.199e-3026.75TRPM8 channel-associated factor 2 [Source:NCBI gen... [more]
ENSXETT00000045039.11.960e-3026.75TRPM8 channel-associated factor 2 [Source:NCBI gen... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tcaf37.349e-3428.84TRPM8 channel-associated factor 3 [Source:MGI Symb... [more]
Tcaf23.573e-3025.86TRPM8 channel-associated factor 2 [Source:MGI Symb... [more]
Tcaf19.036e-2925.27TRPM8 channel-associated factor 1 [Source:MGI Symb... [more]
Tcaf19.036e-2925.27TRPM8 channel-associated factor 1 [Source:MGI Symb... [more]
Tcaf19.036e-2925.27TRPM8 channel-associated factor 1 [Source:MGI Symb... [more]
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BLAST of Peptidase M60 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6QR59|TCAF3_MOUSE5.140e-3328.84TRPM8 channel-associated factor 3 OS=Mus musculus ... [more]
sp|Q5XHI4|TCAF_XENLA2.366e-3227.37TRPM8 channel-associated factor homolog OS=Xenopus... [more]
sp|A6QLU7|TCAF2_BOVIN1.442e-3127.42TRPM8 channel-associated factor 2 OS=Bos taurus OX... [more]
sp|Q6P6V7|TCAF3_RAT9.959e-3127.76TRPM8 channel-associated factor 3 OS=Rattus norveg... [more]
sp|A6NFQ2|TCAF2_HUMAN2.573e-3027.03TRPM8 channel-associated factor 2 OS=Homo sapiens ... [more]
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BLAST of Peptidase M60 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
V3ZHF73.867e-6230.34Peptidase M60 domain-containing protein OS=Lottia ... [more]
A0A1S3I0H42.002e-5830.87TRPM8 channel-associated factor 2-like OS=Lingula ... [more]
R7UHN95.042e-5725.99Peptidase M60 domain-containing protein OS=Capitel... [more]
V4A0M85.090e-5730.47Peptidase M60 domain-containing protein OS=Lottia ... [more]
A0A1S3KG341.774e-5629.69TRPM8 channel-associated factor 2 OS=Lingula ungui... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-210b2.37.398e-2625.77si:ch211-210b2.3 [Source:ZFIN;Acc:ZDB-GENE-131122-... [more]
ENSAMXT00000018418.22.432e-2337.95plasminogen [Source:NCBI gene;Acc:103036400][more]
ENSAMXT00000018419.22.841e-2337.95plasminogen [Source:NCBI gene;Acc:103036400][more]
hgfb3.421e-2233.33hepatocyte growth factor [Source:NCBI gene;Acc:103... [more]
ENSAMXT00000051129.11.172e-1930.00pep primary_assembly:Astyanax_mexicanus-2.0:1:5658... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000976.11.621e-3026.15pep scaffold:Pmarinus_7.0:GL479898:35358:38300:-1 ... [more]
ENSPMAT00000003778.18.061e-2231.75pep scaffold:Pmarinus_7.0:GL482904:4425:14035:-1 g... [more]
hgfb1.626e-1527.18hepatocyte growth factor b [Source:ZFIN;Acc:ZDB-GE... [more]
ENSPMAT00000001728.14.444e-1138.10pep scaffold:Pmarinus_7.0:GL477419:32233:56220:-1 ... [more]
ENSPMAT00000005055.18.947e-932.32pep scaffold:Pmarinus_7.0:GL479085:195887:200886:-... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO397634.322e-1340.26Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO289262.321e-1138.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO301934.976e-828.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO439933.169e-732.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO482153.524e-730.67Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-210b2.31.855e-2626.18TRPM8 channel-associated factor homolog [Source:NC... [more]
plat8.696e-1832.80plasminogen activator, tissue type [Source:NCBI ge... [more]
plat1.199e-1732.80plasminogen activator, tissue type [Source:NCBI ge... [more]
plat1.262e-1732.80plasminogen activator, tissue type [Source:NCBI ge... [more]
hgfb2.344e-1631.40hepatocyte growth factor [Source:NCBI gene;Acc:101... [more]
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BLAST of Peptidase M60 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026283.10.000e+099.26SMESG000026283.1[more]
SMESG000026302.10.000e+055.13SMESG000026302.1[more]
SMESG000026300.10.000e+044.21SMESG000026300.1[more]
SMESG000026300.10.000e+044.21SMESG000026300.1[more]
SMESG000026377.10.000e+045.14SMESG000026377.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014608 ID=SMED30014608|Name=Peptidase M60 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3429bp
ATTTTCTCATGTTTTATTTTAAAAGAAATTTATATTTTCAAATATTTTTA
TTGCTGAATTACGCGATATTTCCGAATTTATCAGCAACTTATTTTACAGA
AATCAAAAAATGCAAAATAACTTCACATGGCAGTGAGTATGTTGGGAATG
TTGAAAAAACATTTTCCGGGAAAAAGTGCATTAAATGGACAATTGAATTG
CTACGGCGACTGAACCTAGAGGAAAAACATTTTTTGGATCTCGAAAGCGT
TTCACCGCATAATTTCTGTAGAAATCCAAATCTTTATCCAAAAGGTCCAT
GGTGTGTAACTGATGAAAAGGCTTTGCAATGGGAGCATTGCGACATTCCT
TTTTGCGTCAAAGATAATAATTGGTGTAAAGACACAGCTGAAGGCCAGGA
GTATTTTGGTAGCTTAAATTTAACTGAATCTGGAAAGGATTGTAAATTAT
GGAGCTTGCAAAACAAATTCCCTGCAAACGGGTTTCCGGAGATGAGTCTT
CAAAATGCAGAAAATTACTGTCGAAGTCCTGATGGCTATTCAAAAGGTCC
TTGGTGTTATGTCGATGACAGCAATATTAAATGGGACAACTGCTATGTAC
CTCAATGTGAAATTAGTTTCAGTCAAATAGAGTATTTTGGGGGTTACGAT
TTCCTGAAAACTTTGAAAGACCATTTAAATGTCATTCAATTGAAGAACAT
TGTCAAAACAATTACATTTATTGTAGTAATAGACCTTACCAAATGGAGCC
TATACGCGCAAAGTAGAACAACGTTATGGGCTACTGGTTATGACCCTCGA
TGTCAAAAAGATTTCAACGAATATGAGTTCTGCGGCCTGTCTAAATCAAC
TAAATTGAAAAATCAAATAACTTTAATTTACAAATGTAATAAACCAATGA
AAAATTTAATTTTCATTTCTCTACTAATGAAAACTGAATACAAAATAGAG
TACAGATTGGGAGAAAATTATGAAATTAAAAATGTCCTAAGCTCAAACTT
GAAGGAATATCAAGAAAAGGTTGACAAATATTTAAAAGTGCATCAAACTA
ATATTATTTCCATGCCAAGTTGTTCAAATTTCGGGAGTTTCCTTATTCTT
AAAGACAATGCTTTCCCGTATGCAATAACATCGGATAATGATGTCGTTTT
TGTAATCTCCGAGATCGGAAAAGGTCGAATTGGTTTGATAGCCTGTGATA
CCATTACCACAGACTATGTACCCTTTAAAGATTACCTTAAAACGATTTAC
GGCGAGAATCAATTAAACCCAGTACTATTAACCGGAGATATAATCAAAAA
GTTGCATGATGATAGCATGAAAGGTATAAGAATGACAATGCAATTGATAT
GGAATGCTCATTATGAAATCGAGGAGTCTTTTGTAAAAAAACTAGTAGAT
TTAATAAAATACGGATTAAAAATCGTAGTAGGACTTAAACCATGGGCGTA
TAACTATTATCGGAAAAACTTGAAAAGTAGTGAACTCGTAACGCTCTTTA
AAGCCTCGGGAGTATGGCCATCGTTAAAGACAATAAATGGAGACCTCAAA
ATTGTGAACAGGGATAATATTGCAGCTTTTCCTCTCAATCACCTCCTTGA
ATTTGCGAAAACAAACCTCATAAATCTTAATGCCGTTTCTCTATCAATCG
GAAATATTGTCTCTATTTCGCCTTTTCAGATTATGGGAAGTCGAGATGAA
TTCGCAGAAATAACTAAAAATATTCAAGAATTAATTGAAATTTATTCTCA
TCATGTCAAATCCTTCATACCTTGCTTAGATAAAACAGTGACAGAAAGAA
CTGAAAGGAATTTGATATATTTCTATAGTGCTCTCTTAATTTATCCTTTC
AACGGACTAATGGTTGCTCCGGGTGTCTCATTTTATCCTGGAAATATTCA
ATCTGACAATTTCCGACAACTTATTGTTCAAATGGCTTTGAATCTTAAAT
TTAAGGACGAAACTTCACAACTTGGTTTGTATTTACCTCCGGGAAAGATT
GGCACAGCTCTATTTAAGAATTCCAAATCTCAAACATGTGTATTGTACCT
AAATTTTCATCTTTTTGATATTTATCAAAAGGACGAATATTATAAATGGC
CAGTAAGTAAGAGAAGTATTTTGTTAAGCATTAATGAAATGCAGACCTTT
TCTTTTCCGTTTGGTGGTCTTTTGTCCATCTCTTGCGCTTTAGATAATGA
TAAAGTTGATATGGTTTTTTCTGGTGTCATTCAAGTTCCGGTATTTGATA
GAAGAGATTCAGATAACAATAAGAATTGGAATGTCCTTCAAAGATCGAAA
GCACCATGGGCAGTTCTTGCCGGTGATTCATTTGTGTTTGTTGTTTCTAT
TAATACTGTGAGGAATCTTGCACAGCCACATAAAGTTTTAGAATTATTTG
ATTTAATGGTCAAAGAAGAATATCATTTAAGAGGAATAGCTGTTCAAAGT
AAACGTAGAGAACTTATTTCTACTGATATACAAATTGAGGCTGGATATAT
GCACGCGGGATATCCGATTGGGACAATGATACAGCATGTGACTAATCCTT
CATATAAATCTAAATTTTTGTTTGATGTAGGAAACATGATGATTGAAGGA
AGATGGGGTGTACTTCATGAAATCGGGCATAATTTGCAAGATTCTTCTTT
CACTTTTACTGGAACAGTAGATGTGACGAATAATATTTTTACACTTGTGG
GGGTAGAGTCGCTTACCGGAAAGAGTTCATGGTCTCAAAATGAAGGATGG
GCCTCTGATAGGTCATATATTAATAGATTTGTTACGTATCTTAATGAAGG
AAAATTCGATGAATGGAAAAATGAGCCATTTTTAGCTTTAGGTGTCTATG
CGCAATTGAGATCAAATTTTGGGTGGGAATTGTACTATCAAGTTTTCCGA
CAATATAATGCTCGCGCGATATTTATTAAAAGAATGTATGATACAGAAAA
AATTCAACAGTGGATTTTGTTGACTTCTATTCATGCGAAAATGAATCTGT
GTCCCTTATACGATTTTTGGAGGTGGCCAATTGATTCAAACACCAATCAA
GAATTATTAAAATATCCTTATTTTTTTCCGGATGATTTTCTTACAAAGTT
AGTATCTAACAAAGTTGATAAGATAATCATGAAATACGGTGGATCATTGG
TAGTCAAACGGCAGCCCGCAAAACTTGAAGAAATGATTGGCCCATTAAAA
AACAAAACATCGGTTGACGGGGAGAGAATAATTGAAAAAATTACAATATT
AAATTAATTTAATTTGTATTTTTTAACTTGATATTTATTATAATAATTTT
GAGTTTCAAATCATATTTTTTTAGTCTTTACATCTTGATAATGTATTTGC
CATTAGCAGTTATATATTTTTTGTTATTATATTTTTGGTTTTTATTACCA
GTCAGTACTAGTTTCTGATTCTTCGATAT
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protein sequence of SMED30014608-orf-1

>SMED30014608-orf-1 ID=SMED30014608-orf-1|Name=SMED30014608-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1085bp
FLMFYFKRNLYFQIFLLLNYAIFPNLSATYFTEIKKCKITSHGSEYVGNV
EKTFSGKKCIKWTIELLRRLNLEEKHFLDLESVSPHNFCRNPNLYPKGPW
CVTDEKALQWEHCDIPFCVKDNNWCKDTAEGQEYFGSLNLTESGKDCKLW
SLQNKFPANGFPEMSLQNAENYCRSPDGYSKGPWCYVDDSNIKWDNCYVP
QCEISFSQIEYFGGYDFLKTLKDHLNVIQLKNIVKTITFIVVIDLTKWSL
YAQSRTTLWATGYDPRCQKDFNEYEFCGLSKSTKLKNQITLIYKCNKPMK
NLIFISLLMKTEYKIEYRLGENYEIKNVLSSNLKEYQEKVDKYLKVHQTN
IISMPSCSNFGSFLILKDNAFPYAITSDNDVVFVISEIGKGRIGLIACDT
ITTDYVPFKDYLKTIYGENQLNPVLLTGDIIKKLHDDSMKGIRMTMQLIW
NAHYEIEESFVKKLVDLIKYGLKIVVGLKPWAYNYYRKNLKSSELVTLFK
ASGVWPSLKTINGDLKIVNRDNIAAFPLNHLLEFAKTNLINLNAVSLSIG
NIVSISPFQIMGSRDEFAEITKNIQELIEIYSHHVKSFIPCLDKTVTERT
ERNLIYFYSALLIYPFNGLMVAPGVSFYPGNIQSDNFRQLIVQMALNLKF
KDETSQLGLYLPPGKIGTALFKNSKSQTCVLYLNFHLFDIYQKDEYYKWP
VSKRSILLSINEMQTFSFPFGGLLSISCALDNDKVDMVFSGVIQVPVFDR
RDSDNNKNWNVLQRSKAPWAVLAGDSFVFVVSINTVRNLAQPHKVLELFD
LMVKEEYHLRGIAVQSKRRELISTDIQIEAGYMHAGYPIGTMIQHVTNPS
YKSKFLFDVGNMMIEGRWGVLHEIGHNLQDSSFTFTGTVDVTNNIFTLVG
VESLTGKSSWSQNEGWASDRSYINRFVTYLNEGKFDEWKNEPFLALGVYA
QLRSNFGWELYYQVFRQYNARAIFIKRMYDTEKIQQWILLTSIHAKMNLC
PLYDFWRWPIDSNTNQELLKYPYFFPDDFLTKLVSNKVDKIIMKYGGSLV
VKRQPAKLEEMIGPLKNKTSVDGERIIEKITILN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000025nervous system
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR031161Peptidase_M60_dom
IPR000001Kringle
IPR018056Kringle_CS
IPR013806Kringle-like
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 7..26