1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014607 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Human
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 584.334 bits (1505), Expect = 0.000e+0 Identity = 332/831 (39.95%), Postives = 485/831 (58.36%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
K VKW++ + I+ D FFF W TE++++SL++D R G++AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 27 KFVKWDDDSTIVTPIILRT--DPQGFFFYWTDQNKETELLDLSLVKDARCGRHAKAPKDPKLRELLDV--GNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 850
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Human
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 584.334 bits (1505), Expect = 0.000e+0 Identity = 332/831 (39.95%), Postives = 487/831 (58.60%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
K VKW++ + T + + + D FFF W TE++++SL++D R G++AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 27 KFVKWDD--DSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGRHAKAPKDPKLRELLDV--GNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 850
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Human
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 584.334 bits (1505), Expect = 0.000e+0 Identity = 332/831 (39.95%), Postives = 485/831 (58.36%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
K VKW++ + I+ D FFF W TE++++SL++D R G++AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 27 KFVKWDDDSTIVTPIILRT--DPQGFFFYWTDQNKETELLDLSLVKDARCGRHAKAPKDPKLRELLDV--GNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 850
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Human
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 562.377 bits (1448), Expect = 0.000e+0 Identity = 311/774 (40.18%), Postives = 453/774 (58.53%), Query Frame = 3
Query: 264 KDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
+DPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 2 QDPKLRELLDV--GNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 770
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Human
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0 Identity = 311/774 (40.18%), Postives = 453/774 (58.53%), Query Frame = 3
Query: 264 KDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
+DPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 2 QDPKLRELLDV--GNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 770
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Celegans
Match: egl-8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 [Source:UniProtKB/Swiss-Prot;Acc:G5EBH0]) HSP 1 Score: 284.648 bits (727), Expect = 3.413e-79 Identity = 137/303 (45.21%), Postives = 196/303 (64.69%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMM-PSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
T I P +SS+VNY P F F+ A+ N+ F S E + L ++ E VN+NKRQ SRIYPKG RVDSSNFLPQ+FWNAGCQ+V+LNFQ+ D+ MQLN+G FE GYLLKP +R DR FDPF ES VDG++A + + SG F+SD+ + Y+E+EM+GLP DT+RK +K + GL P + + F+K++ P++A LR EN K +GQ LP+D ++ GYR I+L+++++QS ++ P + + I YVP ++ AL++P AFLSE+++R + L + D
Sbjct: 556 ATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVD 858 HSP 2 Score: 253.832 bits (647), Expect = 5.200e-69 Identity = 153/424 (36.08%), Postives = 232/424 (54.72%), Query Frame = 3
Query: 141 VNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKIL-DLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKK--IWAKICLELPDTLEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKG-MEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
V +DE+ FF W ++++ I + R G PKD +I+ +L + E + +ER I I G D VN+ + V+++ E I +K + R+ C L K W + + + I ++ K FS K ++ + L D + + + TFE+F +Y K+ + ++ ++ K++ E K+ NF+N+ QRD RLNEIL+P + + L+ K+E+D+ ++G +S G ++++ S+EN + E+ DM+QP HY+INSSHNTYLTG Q GKSS+EIYRQVLLSG RCIELD WDG G + EPI+THG MCT V +DV+ I ++AF SDFPV+LSFENHC+ + + + +L +P E PL+PGV LPSP
Sbjct: 37 AQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGG---LPKDGRIMFELEQRGASETI--AERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKE----RLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSP 451
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Celegans
Match: egl-8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 [Source:UniProtKB/Swiss-Prot;Acc:G5EBH0]) HSP 1 Score: 284.648 bits (727), Expect = 3.618e-79 Identity = 137/303 (45.21%), Postives = 196/303 (64.69%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMM-PSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
T I P +SS+VNY P F F+ A+ N+ F S E + L ++ E VN+NKRQ SRIYPKG RVDSSNFLPQ+FWNAGCQ+V+LNFQ+ D+ MQLN+G FE GYLLKP +R DR FDPF ES VDG++A + + SG F+SD+ + Y+E+EM+GLP DT+RK +K + GL P + + F+K++ P++A LR EN K +GQ LP+D ++ GYR I+L+++++QS ++ P + + I YVP ++ AL++P AFLSE+++R + L + D
Sbjct: 556 ATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVD 858 HSP 2 Score: 253.832 bits (647), Expect = 5.436e-69 Identity = 153/424 (36.08%), Postives = 232/424 (54.72%), Query Frame = 3
Query: 141 VNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKIL-DLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKK--IWAKICLELPDTLEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKG-MEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
V +DE+ FF W ++++ I + R G PKD +I+ +L + E + +ER I I G D VN+ + V+++ E I +K + R+ C L K W + + + I ++ K FS K ++ + L D + + + TFE+F +Y K+ + ++ ++ K++ E K+ NF+N+ QRD RLNEIL+P + + L+ K+E+D+ ++G +S G ++++ S+EN + E+ DM+QP HY+INSSHNTYLTG Q GKSS+EIYRQVLLSG RCIELD WDG G + EPI+THG MCT V +DV+ I ++AF SDFPV+LSFENHC+ + + + +L +P E PL+PGV LPSP
Sbjct: 37 AQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGG---LPKDGRIMFELEQRGASETI--AERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKE----RLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSP 451
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Celegans
Match: egl-8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 [Source:UniProtKB/Swiss-Prot;Acc:G5EBH0]) HSP 1 Score: 284.648 bits (727), Expect = 5.326e-79 Identity = 137/303 (45.21%), Postives = 196/303 (64.69%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMM-PSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
T I P +SS+VNY P F F+ A+ N+ F S E + L ++ E VN+NKRQ SRIYPKG RVDSSNFLPQ+FWNAGCQ+V+LNFQ+ D+ MQLN+G FE GYLLKP +R DR FDPF ES VDG++A + + SG F+SD+ + Y+E+EM+GLP DT+RK +K + GL P + + F+K++ P++A LR EN K +GQ LP+D ++ GYR I+L+++++QS ++ P + + I YVP ++ AL++P AFLSE+++R + L + D
Sbjct: 637 ATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVD 939 HSP 2 Score: 253.832 bits (647), Expect = 7.166e-69 Identity = 153/424 (36.08%), Postives = 232/424 (54.72%), Query Frame = 3
Query: 141 VNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKIL-DLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKK--IWAKICLELPDTLEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKG-MEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
V +DE+ FF W ++++ I + R G PKD +I+ +L + E + +ER I I G D VN+ + V+++ E I +K + R+ C L K W + + + I ++ K FS K ++ + L D + + + TFE+F +Y K+ + ++ ++ K++ E K+ NF+N+ QRD RLNEIL+P + + L+ K+E+D+ ++G +S G ++++ S+EN + E+ DM+QP HY+INSSHNTYLTG Q GKSS+EIYRQVLLSG RCIELD WDG G + EPI+THG MCT V +DV+ I ++AF SDFPV+LSFENHC+ + + + +L +P E PL+PGV LPSP
Sbjct: 37 AQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGG---LPKDGRIMFELEQRGASETI--AERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKE----RLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSP 451
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Celegans
Match: egl-8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 [Source:UniProtKB/Swiss-Prot;Acc:G5EBH0]) HSP 1 Score: 284.648 bits (727), Expect = 5.478e-79 Identity = 137/303 (45.21%), Postives = 196/303 (64.69%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMM-PSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
T I P +SS+VNY P F F+ A+ N+ F S E + L ++ E VN+NKRQ SRIYPKG RVDSSNFLPQ+FWNAGCQ+V+LNFQ+ D+ MQLN+G FE GYLLKP +R DR FDPF ES VDG++A + + SG F+SD+ + Y+E+EM+GLP DT+RK +K + GL P + + F+K++ P++A LR EN K +GQ LP+D ++ GYR I+L+++++QS ++ P + + I YVP ++ AL++P AFLSE+++R + L + D
Sbjct: 637 ATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVD 939 HSP 2 Score: 253.832 bits (647), Expect = 6.862e-69 Identity = 153/424 (36.08%), Postives = 232/424 (54.72%), Query Frame = 3
Query: 141 VNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKIL-DLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKK--IWAKICLELPDTLEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKG-MEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
V +DE+ FF W ++++ I + R G PKD +I+ +L + E + +ER I I G D VN+ + V+++ E I +K + R+ C L K W + + + I ++ K FS K ++ + L D + + + TFE+F +Y K+ + ++ ++ K++ E K+ NF+N+ QRD RLNEIL+P + + L+ K+E+D+ ++G +S G ++++ S+EN + E+ DM+QP HY+INSSHNTYLTG Q GKSS+EIYRQVLLSG RCIELD WDG G + EPI+THG MCT V +DV+ I ++AF SDFPV+LSFENHC+ + + + +L +P E PL+PGV LPSP
Sbjct: 37 AQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGG---LPKDGRIMFELEQRGASETI--AERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKE----RLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSP 451
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Celegans
Match: egl-8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta egl-8 [Source:UniProtKB/Swiss-Prot;Acc:G5EBH0]) HSP 1 Score: 284.263 bits (726), Expect = 5.485e-79 Identity = 137/303 (45.21%), Postives = 196/303 (64.69%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMM-PSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
T I P +SS+VNY P F F+ A+ N+ F S E + L ++ E VN+NKRQ SRIYPKG RVDSSNFLPQ+FWNAGCQ+V+LNFQ+ D+ MQLN+G FE GYLLKP +R DR FDPF ES VDG++A + + SG F+SD+ + Y+E+EM+GLP DT+RK +K + GL P + + F+K++ P++A LR EN K +GQ LP+D ++ GYR I+L+++++QS ++ P + + I YVP ++ AL++P AFLSE+++R + L + D
Sbjct: 584 ATTNIHPLLSSLVNYTHPVKFSGFDVAEANNLHFHMSSFSESTGLGYLKQSAPEFVNYNKRQSSRIYPKGARVDSSNFLPQIFWNAGCQMVSLNFQTPDVYMQLNMGKFEYNGGSGYLLKPDFLRRPDRTFDPFSESPVDGVIAAHCSVRVISGQFLSDRKIGTYVEVEMYGLPTDTIRKEHKTKVIPGNGLNPVYNEDPFVFRKVVLPELAVLRFAVYDENGKQLGQRILPLDGLQAGYRHISLRSDTNQSFILSPVLFVQIVIKTYVPDELSGLVDALADPRAFLSEQKKRQEALAHMGVD 886 HSP 2 Score: 240.35 bits (612), Expect = 1.157e-64 Identity = 153/452 (33.85%), Postives = 233/452 (51.55%), Query Frame = 3
Query: 141 VNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKIL-DLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKK--IWAKICLELPDTLEINMLTKYFSQNKDDR---EKLED----------------------------AXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKG-MEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
V +DE+ FF W ++++ I + R G PKD +I+ +L + E + +ER I I G D VN+ + V+++ E I +K + R+ C L K W + + + I ++ K FS K ++ + L D + + + + TFE+F +Y K+ + ++ ++ K++ E K+ NF+N+ QRD RLNEIL+P + + L+ K+E+D+ ++G +S G ++++ S+EN + E+ DM+QP HY+INSSHNTYLTG Q GKSS+EIYRQVLLSG RCIELD WDG G + EPI+THG MCT V +DV+ I ++AF SDFPV+LSFENHC+ + + + +L +P E PL+PGV LPSP
Sbjct: 37 AQVRIDENGFFLRWLIEGKDAVVLDMGQIWEARTGG---LPKDGRIMFELEQRGASETI--AERTIWITHGQDLVNVQSFFLVAESVELAKTCRAGINDILKSSRIRHVCPTTQLMKYHTWLTMNVNERRKIPIKLIIKTFSSGKPEKMVQKCLNDLGLGGDKYTPARVINRSMGKKFRNFYKCSRGRKRKEREELDVDILTFEKFQRLYNKICPRTEVQELFVKLSGQKEYLTKE----RLINFLNEEQRDPRLNEILFPFFDSQRIVALLKKHENDIKYQEDGKMSGDGFLRFLMSDENPPVFLDRIEMFMDMDQPLCHYYINSSHNTYLTGRQYGGKSSSEIYRQVLLSGCRCIELDCWDGTGENKGEPIITHGKAMCTDVFFKDVLVQIRDTAFARSDFPVVLSFENHCSKSNQLKMAKYCMDIFGDMLLSKPFEDAPLDPGVSLPSP 479
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Fly
Match: Plc21C (gene:FBgn0004611 transcript:FBtr0330673) HSP 1 Score: 325.865 bits (834), Expect = 4.473e-93 Identity = 149/295 (50.51%), Postives = 206/295 (69.83%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
+IS++VNYV+P F SFE A++KN +E S E A L E P+E VN+NK QLSR+YP GTR DSSNF+PQLFWNAGCQLVALNFQ+LDL MQLNLG+FE R GYLLKP MR SDR DPF ES VDGI+A ++ + + SG F++DK ++E++M+GLPADTVRK+++ ++ G+ P + + F+K++ P++A +RI A E K +G LPV + PGYR + L++E Q + + S+ + + DYVP D+ +FA AL+NPI + SE E+R QL +LT++
Sbjct: 537 EISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDE 831 HSP 2 Score: 119.783 bits (299), Expect = 6.970e-27 Identity = 86/282 (30.50%), Postives = 149/282 (52.84%), Query Frame = 3
Query: 90 EKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIK 920
EK ++W++ + + V + VD FF W +I++I+ IRD+R G+YAK PKD K+ + T + +E E+ +++C G+DFVN+++ NF ++ D +I N ++ L+K K+CL++ + + + + K F+QNK+DR+++E D K +I S F FE F +Y+ L + +E ++ I + + I + F+N+ QRD RLNEILYP ++KELI +YE + +Q G +S G ++
Sbjct: 24 EKFIRWDD--DSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLE--EKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSI--AQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLR 299 HSP 3 Score: 105.531 bits (262), Expect = 1.560e-22 Identity = 45/97 (46.39%), Postives = 68/97 (70.10%), Query Frame = 3
Query: 1101 RCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
RC+ELD W+G+ +EP++ HG+T + +DV++AI ESAFK S++PVILSFENHC +Q + + +E + +L +PL+S+PLEP + LP P
Sbjct: 299 RCVELDFWNGR--TEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPP 393
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Fly
Match: Plc21C (gene:FBgn0004611 transcript:FBtr0330674) HSP 1 Score: 325.479 bits (833), Expect = 8.894e-93 Identity = 149/295 (50.51%), Postives = 206/295 (69.83%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
+IS++VNYV+P F SFE A++KN +E S E A L E P+E VN+NK QLSR+YP GTR DSSNF+PQLFWNAGCQLVALNFQ+LDL MQLNLG+FE R GYLLKP MR SDR DPF ES VDGI+A ++ + + SG F++DK ++E++M+GLPADTVRK+++ ++ G+ P + + F+K++ P++A +RI A E K +G LPV + PGYR + L++E Q + + S+ + + DYVP D+ +FA AL+NPI + SE E+R QL +LT++
Sbjct: 598 EISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDE 892 HSP 2 Score: 306.22 bits (783), Expect = 3.415e-86 Identity = 170/439 (38.72%), Postives = 264/439 (60.14%), Query Frame = 3
Query: 90 EKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
EK ++W++ + + V + VD FF W +I++I+ IRD+R G+YAK PKD K+ + T + +E E+ +++C G+DFVN+++ NF ++ D +I N ++ L+K K+CL++ + + + + K F+QNK+DR+++E D K +I S F FE F +Y+ L + +E ++ I + + I + F+N+ QRD RLNEILYP ++KELI +YE + +Q G +S G ++Y+ ++N I+ S +L DM+QP +HYFINSSHNTYLTGHQLTGKSS EIYRQ LL+G RC+ELD W+G+ +EP++ HG+T + +DV++AI ESAFK S++PVILSFENHC +Q + + +E + +L +PL+S+PLEP + LP P
Sbjct: 24 EKFIRWDD--DSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLE--EKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSI--AQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGR--TEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPP 454
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Fly
Match: Plc21C (gene:FBgn0004611 transcript:FBtr0078050) HSP 1 Score: 325.479 bits (833), Expect = 9.014e-93 Identity = 149/295 (50.51%), Postives = 206/295 (69.83%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
+IS++VNYV+P F SFE A++KN +E S E A L E P+E VN+NK QLSR+YP GTR DSSNF+PQLFWNAGCQLVALNFQ+LDL MQLNLG+FE R GYLLKP MR SDR DPF ES VDGI+A ++ + + SG F++DK ++E++M+GLPADTVRK+++ ++ G+ P + + F+K++ P++A +RI A E K +G LPV + PGYR + L++E Q + + S+ + + DYVP D+ +FA AL+NPI + SE E+R QL +LT++
Sbjct: 598 EISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDE 892 HSP 2 Score: 306.22 bits (783), Expect = 3.526e-86 Identity = 170/439 (38.72%), Postives = 264/439 (60.14%), Query Frame = 3
Query: 90 EKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
EK ++W++ + + V + VD FF W +I++I+ IRD+R G+YAK PKD K+ + T + +E E+ +++C G+DFVN+++ NF ++ D +I N ++ L+K K+CL++ + + + + K F+QNK+DR+++E D K +I S F FE F +Y+ L + +E ++ I + + I + F+N+ QRD RLNEILYP ++KELI +YE + +Q G +S G ++Y+ ++N I+ S +L DM+QP +HYFINSSHNTYLTGHQLTGKSS EIYRQ LL+G RC+ELD W+G+ +EP++ HG+T + +DV++AI ESAFK S++PVILSFENHC +Q + + +E + +L +PL+S+PLEP + LP P
Sbjct: 24 EKFIRWDD--DSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLE--EKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSI--AQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGR--TEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPP 454
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Fly
Match: Plc21C (gene:FBgn0004611 transcript:FBtr0078049) HSP 1 Score: 325.479 bits (833), Expect = 1.005e-92 Identity = 149/295 (50.51%), Postives = 206/295 (69.83%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
+IS++VNYV+P F SFE A++KN +E S E A L E P+E VN+NK QLSR+YP GTR DSSNF+PQLFWNAGCQLVALNFQ+LDL MQLNLG+FE R GYLLKP MR SDR DPF ES VDGI+A ++ + + SG F++DK ++E++M+GLPADTVRK+++ ++ G+ P + + F+K++ P++A +RI A E K +G LPV + PGYR + L++E Q + + S+ + + DYVP D+ +FA AL+NPI + SE E+R QL +LT++
Sbjct: 598 EISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDE 892 HSP 2 Score: 306.22 bits (783), Expect = 3.545e-86 Identity = 170/439 (38.72%), Postives = 264/439 (60.14%), Query Frame = 3
Query: 90 EKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
EK ++W++ + + V + VD FF W +I++I+ IRD+R G+YAK PKD K+ + T + +E E+ +++C G+DFVN+++ NF ++ D +I N ++ L+K K+CL++ + + + + K F+QNK+DR+++E D K +I S F FE F +Y+ L + +E ++ I + + I + F+N+ QRD RLNEILYP ++KELI +YE + +Q G +S G ++Y+ ++N I+ S +L DM+QP +HYFINSSHNTYLTGHQLTGKSS EIYRQ LL+G RC+ELD W+G+ +EP++ HG+T + +DV++AI ESAFK S++PVILSFENHC +Q + + +E + +L +PL+S+PLEP + LP P
Sbjct: 24 EKFIRWDD--DSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLE--EKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSI--AQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGR--TEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPP 454
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Fly
Match: Plc21C (gene:FBgn0004611 transcript:FBtr0078048) HSP 1 Score: 325.479 bits (833), Expect = 1.095e-92 Identity = 149/295 (50.51%), Postives = 206/295 (69.83%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
+IS++VNYV+P F SFE A++KN +E S E A L E P+E VN+NK QLSR+YP GTR DSSNF+PQLFWNAGCQLVALNFQ+LDL MQLNLG+FE R GYLLKP MR SDR DPF ES VDGI+A ++ + + SG F++DK ++E++M+GLPADTVRK+++ ++ G+ P + + F+K++ P++A +RI A E K +G LPV + PGYR + L++E Q + + S+ + + DYVP D+ +FA AL+NPI + SE E+R QL +LT++
Sbjct: 598 EISALVNYVQPIHFSSFENAEKKNRCYEMSSFDEKQATTLLKERPIEFVNYNKHQLSRVYPAGTRFDSSNFMPQLFWNAGCQLVALNFQTLDLAMQLNLGIFEYNARSGYLLKPEFMRRSDRRLDPFAESTVDGIIAGTVSITVLSGQFLTDKRANTFVEVDMYGLPADTVRKKFRTKTVRDNGMNPLYDEEPFVFKKVVLPELASIRIAAYEEGGKLIGHRVLPVIGLCPGYRHVNLRSEVGQPIALASLFLCVVVKDYVPDDLSNFAEALANPIKYQSELEKRDIQLSVLTDE 892 HSP 2 Score: 306.22 bits (783), Expect = 4.270e-86 Identity = 170/439 (38.72%), Postives = 264/439 (60.14%), Query Frame = 3
Query: 90 EKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
EK ++W++ + + V + VD FF W +I++I+ IRD+R G+YAK PKD K+ + T + +E E+ +++C G+DFVN+++ NF ++ D +I N ++ L+K K+CL++ + + + + K F+QNK+DR+++E D K +I S F FE F +Y+ L + +E ++ I + + I + F+N+ QRD RLNEILYP ++KELI +YE + +Q G +S G ++Y+ ++N I+ S +L DM+QP +HYFINSSHNTYLTGHQLTGKSS EIYRQ LL+G RC+ELD W+G+ +EP++ HG+T + +DV++AI ESAFK S++PVILSFENHC +Q + + +E + +L +PL+S+PLEP + LP P
Sbjct: 24 EKFIRWDD--DSGTGTPVTMRVDAKGFFLYWVDQNNELDILDIATIRDVRTGQYAKRPKDNKLRQIVTLGPQDTLE--EKTVTVCHGSDFVNMTFVNFCCTRRDIAQLWTDGLIKLAYSLAQLNGSAIMFLQKAHTKLCLQVDKSGRIPVKNIIKLFAQNKEDRKRVEKALDVTGLPSGKVDSISVSKFQFEDFYNLYKYLTQRSEVERLFDSIVGNSKRKCMSI--AQLVEFLNKTQRDPRLNEILYPYANPARAKELIQQYEPNKFNAQKGQLSLDGFLRYLMGDDNPIMAPSKLDLCDDMDQPMSHYFINSSHNTYLTGHQLTGKSSVEIYRQCLLAGCRCVELDFWNGR--TEEPVIVHGYTFVPEIFAKDVLEAIAESAFKTSEYPVILSFENHCNPRQQAKIANYCREIFGDMLLDKPLDSHPLEPNMDLPPP 454
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Zebrafish
Match: plcb3 (phospholipase C, beta 3 (phosphatidylinositol-specific) [Source:ZFIN;Acc:ZDB-GENE-030616-594]) HSP 1 Score: 337.035 bits (863), Expect = 6.319e-97 Identity = 162/330 (49.09%), Postives = 222/330 (67.27%), Query Frame = 3
Query: 1464 DSNEQGDGDKGTEI----IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEAN-EREEESDG 2438
D + + D+GT ++S++VNY+EP F+SFE A ++ FE S E + +L +P E V +NK+Q+SRIYPKGTRVDSSN++PQLFWN GCQ+VALNFQ+LDL MQLN+G+FE RCGYLLKP MR +D+ FDPF VDGIVAN++K+ + SG F++DK V Y+E++MFGLPADT RK Y+ +++ L P + F K++ P +A LR+ EN KF+G LPV +RPGY +I LKNE +Q LM+PS+L+Y E+ DY+P + +++A AL+NPI LS +R QL L ED E ++EE DG
Sbjct: 548 DPKKHNNTDEGTAYSEVNATEEMSTLVNYIEPVKFKSFEVAAKRKKFFEMSSFVETKGMDTLKNSPTEFVEYNKKQMSRIYPKGTRVDSSNYMPQLFWNVGCQMVALNFQTLDLAMQLNMGVFEYNGRCGYLLKPEFMRRTDKHFDPFTTDIVDGIVANTVKVKIISGQFLNDKKVGVYVEVDMFGLPADTKRK-YRTKTSNNNSLDPVWDDETFVFNKVVLPTLASLRVAVYEENGKFIGHRILPVSALRPGYHYICLKNELNQPLMLPSLLVYTEVQDYIPNEHQEYAEALTNPIKHLSLLAQRESQLLALMEDPTEGGPTKQEEGDG 876 HSP 2 Score: 263.077 bits (671), Expect = 8.310e-72 Identity = 170/449 (37.86%), Postives = 249/449 (55.46%), Query Frame = 3
Query: 66 ISSNDILTE--KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGA-TXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPD--TLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE 1394
+S N++L K +KW+E E + S++ + VD + FF W T EI+++S IRD R GK+AK PKD +I ++ + + S R +++ G D VN+S+ NF + + D + S+N L K + K+ L++ + + + K FS K LE DFT+E F KL + +E I+ + ++ + +FIN+ QRD RLNE+LYP + D+ ++L+ KYE++ + IS KY+ EEN ++ ++ DMNQP +HYFINSSHNTYLT QLTG SS E+YRQVLL+G RCIELD W G+ E+EPI+THGFTM T +P +DVI+AI ESAFK S +P+ILSFENH ++KQ + + + +L +PL+ PL PG LPSP+
Sbjct: 16 VSVNEVLKRGSKFIKWDE-EANSTPSLITLRVDPNGFFLYWTGGTSMEAEILDMSHIRDTRTGKFAKTPKDQRIREMLFSGKADGNSDS-RLLTVVYGNDLVNISFLNFQAVQDGYCKLWTDELFKLATNMLSQNASRNTFLLKAYTKLKLQVTQDGKIPVKNILKMFSDKKRVEMALEHCGLIFNRVDGIKPD-DFTWEMFQNFLNKLSPRPEVERIFVDLG---SKGKPFLSLDQLMDFINRKQRDSRLNEVLYPPLKRDQVRQLMEKYETNTSQLERDQISLMSFTKYLGGEENTVVPPERLDIIDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCWKGRPPEEEPIITHGFTMTTEIPFKDVIEAIAESAFKTSPYPLILSFENHVDSAKQQAKMAEYCRSMFGDALLIDPLDKYPLVPGQQLPSPQ 458
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Zebrafish
Match: plcb3 (phospholipase C, beta 3 (phosphatidylinositol-specific) [Source:ZFIN;Acc:ZDB-GENE-030616-594]) HSP 1 Score: 337.035 bits (863), Expect = 6.369e-97 Identity = 162/330 (49.09%), Postives = 222/330 (67.27%), Query Frame = 3
Query: 1464 DSNEQGDGDKGTEI----IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEAN-EREEESDG 2438
D + + D+GT ++S++VNY+EP F+SFE A ++ FE S E + +L +P E V +NK+Q+SRIYPKGTRVDSSN++PQLFWN GCQ+VALNFQ+LDL MQLN+G+FE RCGYLLKP MR +D+ FDPF VDGIVAN++K+ + SG F++DK V Y+E++MFGLPADT RK Y+ +++ L P + F K++ P +A LR+ EN KF+G LPV +RPGY +I LKNE +Q LM+PS+L+Y E+ DY+P + +++A AL+NPI LS +R QL L ED E ++EE DG
Sbjct: 548 DPKKHNNTDEGTAYSEVNATEEMSTLVNYIEPVKFKSFEVAAKRKKFFEMSSFVETKGMDTLKNSPTEFVEYNKKQMSRIYPKGTRVDSSNYMPQLFWNVGCQMVALNFQTLDLAMQLNMGVFEYNGRCGYLLKPEFMRRTDKHFDPFTTDIVDGIVANTVKVKIISGQFLNDKKVGVYVEVDMFGLPADTKRK-YRTKTSNNNSLDPVWDDETFVFNKVVLPTLASLRVAVYEENGKFIGHRILPVSALRPGYHYICLKNELNQPLMLPSLLVYTEVQDYIPNEHQEYAEALTNPIKHLSLLAQRESQLLALMEDPTEGGPTKQEEGDG 876 HSP 2 Score: 263.077 bits (671), Expect = 8.893e-72 Identity = 170/449 (37.86%), Postives = 249/449 (55.46%), Query Frame = 3
Query: 66 ISSNDILTE--KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGA-TXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPD--TLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE 1394
+S N++L K +KW+E E + S++ + VD + FF W T EI+++S IRD R GK+AK PKD +I ++ + + S R +++ G D VN+S+ NF + + D + S+N L K + K+ L++ + + + K FS K LE DFT+E F KL + +E I+ + ++ + +FIN+ QRD RLNE+LYP + D+ ++L+ KYE++ + IS KY+ EEN ++ ++ DMNQP +HYFINSSHNTYLT QLTG SS E+YRQVLL+G RCIELD W G+ E+EPI+THGFTM T +P +DVI+AI ESAFK S +P+ILSFENH ++KQ + + + +L +PL+ PL PG LPSP+
Sbjct: 16 VSVNEVLKRGSKFIKWDE-EANSTPSLITLRVDPNGFFLYWTGGTSMEAEILDMSHIRDTRTGKFAKTPKDQRIREMLFSGKADGNSDS-RLLTVVYGNDLVNISFLNFQAVQDGYCKLWTDELFKLATNMLSQNASRNTFLLKAYTKLKLQVTQDGKIPVKNILKMFSDKKRVEMALEHCGLIFNRVDGIKPD-DFTWEMFQNFLNKLSPRPEVERIFVDLG---SKGKPFLSLDQLMDFINRKQRDSRLNEVLYPPLKRDQVRQLMEKYETNTSQLERDQISLMSFTKYLGGEENTVVPPERLDIIDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCWKGRPPEEEPIITHGFTMTTEIPFKDVIEAIAESAFKTSPYPLILSFENHVDSAKQQAKMAEYCRSMFGDALLIDPLDKYPLVPGQQLPSPQ 458
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Zebrafish
Match: PLCB4 (phospholipase C beta 4 [Source:NCBI gene;Acc:100537538]) HSP 1 Score: 285.034 bits (728), Expect = 1.978e-79 Identity = 133/299 (44.48%), Postives = 190/299 (63.55%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEIL 2390
T I P +S+++NY + F F++A+EKN+ S E + L N +E VN+NKRQ+SRIYPKG RVDSSN++PQ+FWNAGCQ+V+LNFQ+ DL MQLN G FE CG+LLKP MR DR FDPF E+ VDG++A + + + SG F+SDK + Y+E++M+GLP DT+RK ++ R GL P + F+K+I P +A LR+ +N K +GQ LP+D ++ GYR I+L+NE ++ L + ++ + YVP ALS+P FLS E+R Q+ +
Sbjct: 544 ATTNIHPYLSNIINYAQAVKFTGFDEAEEKNIHHNMSSFNESVGLGYLRTNAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNFQTPDLAMQLNQGKFEYNGSCGFLLKPDFMRRPDRMFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNAEAFVFRKVILPDLAVLRVAVYDDNNKLIGQRILPLDGLQSGYRHISLRNEYNKPLPLATVFCNIILKTYVPDGFGAIVDALSDPKKFLSICEKRTDQMRAM 842 HSP 2 Score: 281.567 bits (719), Expect = 2.725e-78 Identity = 166/420 (39.52%), Postives = 246/420 (58.57%), Query Frame = 3
Query: 153 VDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICL--ELPDTLEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNP--LEPGVPLPSP 1391
VDE FF +WK+ +++E SLI I + A KDPK+L A L +R I IC+GTD VNLS+ V++ + D + S I+ ++ N C + L+K W ++ + + + +T+ F+ K ++ + L+D SG + ++ FTF++F + +K+ + +E+++ K D + ++ +F +F+N QRD RLNEIL+P + + ++I +YE L GL+S G +++ S+EN + E+ Q+M+ P +HYFINSSHNTYLTG Q GKSS EIYRQVLL+G RC+ELD WDGKG + EPI+THG MCT V +DVI I E+AF S++PVILSFENHCT Q L ++ + +LK+PLE P +EPG PLPSP
Sbjct: 14 VDESGFFLTWKSEGKEGQVLEFSLINSI---RDASALKDPKVLCALEASGKCESNLEDRIICICSGTDLVNLSFMYMVTQNPTTAKEWTDGLRSLIQNTRANNVCPMTCLRKHWMRLSFLTNVNGQIPVRSITRTFASGKTEKGIFQALKDLGLPSGKNDEIQREI-FTFDKFYSLTQKICPRTDIEVLFKKFKGDKGDY---LSVEQFVHFLNDNQRDPRLNEILFPFFNSKCAFQIIQRYEPVKALVCAGLLSCDGFCRFLMSDENAPVFLDRLEVYQEMDHPLSHYFINSSHNTYLTGRQFGGKSSVEIYRQVLLTGCRCVELDCWDGKGEDQEPIITHGKAMCTDVLFKDVIQGIKETAFLTSEYPVILSFENHCTKHQQYKLARYCEDIFGDLLLKQPLEDFPPKMEPGCPLPSP 426
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Zebrafish
Match: CABZ01072157.1 (pep chromosome:GRCz11:17:2348540:2386569:-1 gene:ENSDARG00000090381.3 transcript:ENSDART00000121614.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01072157.1) HSP 1 Score: 275.789 bits (704), Expect = 3.372e-79 Identity = 132/288 (45.83%), Postives = 190/288 (65.97%), Query Frame = 3
Query: 1482 DGDKGTEIII-PQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPI 2342
+G G E+ ++S++VNYV+P F SFE A +KN F S E + +N V+ V +NKRQLSRIYPKGTRVDSSN+ PQ FW+AGCQ VALN+Q++D MQLN+ LFE R GYLLK MR SD+ FDPF + +D I+A++L + ++SG F+SDK V +E+E+ GLP D RK S+T G+ P + + F+K++ P+MA LR++ E KF+G +P+D ++ G++ I L++ES+ LM+PS+ ++ E+ DYVP DF+ AL NP+
Sbjct: 135 EGTAGQEVTAYEEMSALVNYVQPNKFISFENAAKKNRCFVISSFVETKGESLIAKNAVDWVEYNKRQLSRIYPKGTRVDSSNYSPQPFWSAGCQFVALNYQTMDFPMQLNMALFEFNGRTGYLLKHDVMRRSDKKFDPFCDR-IDTIIASTLTIKIYSGQFLSDKAVKTGVEVELIGLPKDPKRKYRTKMSSTANGINPDWNEEPFIFEKVLLPEMAYLRLVVQEEGGKFIGHRIIPLDALQTGFQHIGLRSESNMPLMLPSLFVHIEMKDYVPAAFADFSDALFNPV 421
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Zebrafish
Match: plcd1b (phospholipase C, delta 1b [Source:ZFIN;Acc:ZDB-GENE-030131-9435]) HSP 1 Score: 275.789 bits (704), Expect = 9.454e-79 Identity = 187/571 (32.75%), Postives = 297/571 (52.01%), Query Frame = 3
Query: 666 YEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVP--LPSPEXXXXXXXXXXXXXXXXXETDSTNDS----------------NEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKG------VYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQ-KIIHPKMAKLRILA----STENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDD 2291
Y+ L ++ +++IY + A + +++ + NF+ QR++ ++ + + +LI KYE D ++ G + Y+ E I +Q++ + DMNQP +HYFI+SSHNTYL QL G SS E Y + LL G RC+ELD WDG + EP++ HG+T+ + + +DVI AI E AFK S++PVILS ENHC +Q I+ + L++ IL L + PL +P LP+PE+LKG+ ++K K+ + S E + G+ + ++S +V Y + F E ++EK+ +E S E A++ E+ E + N +LSRIYP G R DSSN+ P WNAGCQ+VALNFQ+ L M LN GLF R GY+LKP +R D +FDP + + +L +++ S + G V ++++++G+PADT + G P + N +FQ ++ P +A +R L ST + +F+GQ LP + +R GY + L + L + ++ + D
Sbjct: 202 YKILTKREEIDVIYGEYA----KTDGLMSANDLLNFLLTEQREE---------VSQNDALQLIEKYELDENAKAKQQMTQDGFLMYLHQPEGLIFNQAHKSVYHDMNQPLSHYFISSSHNTYLMEDQLKGPSSTEAYIRALLKGCRCVELDCWDGS--DGEPVIYHGYTLTSKILFKDVIKAIKEYAFKTSEYPVILSLENHCGVEQ----QKIMAQHLTS-ILGSALVTKPLGDQMPTCLPAPEELKGRFIVKGKRLDKLENAFSDESKMADEESVSEEEDDEGDEENNEKKSGSLKLAKELSDIVIYCKSVPFHGLEDSREKHSFYEMASFKEGKALKLAEESATEYIRHNTDKLSRIYPGGMRTDSSNYNPVPMWNAGCQIVALNFQTPCLEMDLNQGLFYQNGRSGYILKPSYLRERDTEFDPITLTKGPWLKRKTLHVMVISAQQLPKVGKRESAIVDPIVKVQVYGVPADTA--AVETSYIKNNGFNPMW---NENFQLEVCVPDLALVRFLIEDYDSTSDNEFIGQYTLPFNSLRNGYHHVPLLTQDGNLLSSAGLFVHIMVVD 747
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Xenopus
Match: PLCB3 (phospholipase C beta 3 [Source:NCBI gene;Acc:100487267]) HSP 1 Score: 552.362 bits (1422), Expect = 1.778e-177 Identity = 337/812 (41.50%), Postives = 495/812 (60.96%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL--PDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFT---FERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETD---------STNDSNEQGDGDK-------------------GTEIII-----------PQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQL 2381
K +KW+E E ++ S+V + VD F+ W +I++ISLIRD RIGKYAK PKD K+ ++ + E ++ ++ GTD VN+S+ NF++ K + ++ L + + SRN L+K + K+ L++ + + + K F+ +K E ++ + ++ +IK +FT FERFL KL L+ ++ I ++ + + + +F+NQ QRD RLNEIL+P + D+ ++LI KYE + + +S +G +++ +EN+I+ +L+ DMNQP + YFINSSHNTYLT QLTG SS E YRQVLL+G RCIELD W G+ ++EP +THGFTM T +P ++VI+AI ESAFK S FPVILSFENH +SKQ + + + +L +PLE PL+ GV LPSP++L GKIL+KNKK+ S SN D G +II+ ++S++VNYVEP F+SF+ AK++N +E S E A++ L ++P+E V +NK+QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ+LDL MQLN+G+FE R G+LLKP MR +D+ FDPF E+ VDGIVAN++K+ + SG F+S+K V Y+E++MFGLP DT RK ++ +++ P + + F K++ P +A LRI E KFVG LPV IRPGY +I L+NE +Q + +P++L+Y E++DY+P D + +A AL+NPI ++ ++RA+QL
Sbjct: 27 KFIKWDE--EPSSKSLVTLRVDAMGFYLYWTCPNMEVDILDISLIRDTRIGKYAKIPKDMKMREILGFTAPEH-RPEDKLFTVVHGTDIVNISFLNFMAVQEDTAKVWTEELFKLAHNILAQNSSRN----TFLQKSYTKLKLQVNQDGRIPVKNILKMFAADKKRVETALESCGLNFNRGDSIKPEEFTLDIFERFL---NKLCLRPDIDKILLELGAKGK---PYLTLEQLMDFLNQKQRDPRLNEILFPPLKRDQVRQLIEKYEPNRQFLERDQMSMEGFSRFLGGDENSIVPPEILDLSDDMNQPLSSYFINSSHNTYLTAGQLTGNSSVETYRQVLLTGCRCIELDCWKGRPQDEEPFITHGFTMTTEIPFKEVIEAIAESAFKTSPFPVILSFENHVDSSKQQAKMAEYCRNIFGDALLIDPLEKYPLQAGVQLPSPQELMGKILVKNKKRHKVAAKGPEGSVKRRVSEQTSNTYSDSSSVFNSIEEKPPRYTEPRKSIGKKIILLGTASSEVNATEEMSTLVNYVEPVKFKSFDVAKKRNKYYEMSSFVETKALEQLTKSPMEFVEYNKKQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTLDLPMQLNVGIFEYNRRSGFLLKPEFMRRTDKHFDPFTENIVDGIVANTVKIKIISGQFLSEKRVGIYVEVDMFGLPVDTKRK-FRTKTSQGNSFNPVWDEEPFIFPKVVLPTLASLRIAVFEEGGKFVGHRILPVSAIRPGYHYICLRNELNQPVCLPALLVYTEVNDYIPDDHQGYANALTNPIKHINLMDQRAKQL 824
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Xenopus
Match: PLCB3 (phospholipase C beta 3 [Source:NCBI gene;Acc:100487267]) HSP 1 Score: 528.48 bits (1360), Expect = 4.608e-167 Identity = 311/754 (41.25%), Postives = 449/754 (59.55%), Query Frame = 3
Query: 243 GKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINMLTKYFSQNK---DDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXX--------------------------------XXETDSTNDSNEQGDGDKGTEI-IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQL 2381
GKYAK PKD K+ ++ + E ++ ++ GTD VN+S+ NF++ K + ++ L + + SRN L+K + + L P + +FS K D++++E A+ S F R I K K L E + +F+NQ QRD RLNEIL+P + D+ ++LI KYE + + +S +G +++ +EN+I+ +L+ DMNQP + YFINSSHNTYLT QLTG SS E YRQVLL+G RCIELD W G+ ++EP +THGFTM T +P ++VI+AI ESAFK S FPVILSFENH +SKQ + + + +L +PLE PL+ GV LPSP++L GKIL+KNKK+ E + +D + +G +E+ ++S++VNYVEP F+SF+ AK++N +E S E A++ L ++P+E V +NK+QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ+LDL MQLN+G+FE R G+LLKP MR +D+ FDPF E+ VDGIVAN++K+ + SG F+S+K V Y+E++MFGLP DT RK ++ +++ P + + F K++ P +A LRI E KFVG LPV IRPGY +I L+NE +Q + +P++L+Y E++DY+P D + +A AL+NPI ++ ++RA+QL
Sbjct: 48 GKYAKIPKDMKMREILGFTAPEH-RPEDKLFTVVHGTDIVNISFLNFMAVQEDTAKVWTEELFKLAHNILAQNSSRN----TFLQKSFYLLFLTRPPLPCAETVPCFFSILKMFAADKKRVETALESC----------GLNFNRVKPIGAK-----------GKPYLTLE---------QLMDFLNQKQRDPRLNEILFPPLKRDQVRQLIEKYEPNRQFLERDQMSMEGFSRFLGGDENSIVPPEILDLSDDMNQPLSSYFINSSHNTYLTAGQLTGNSSVETYRQVLLTGCRCIELDCWKGRPQDEEPFITHGFTMTTEIPFKEVIEAIAESAFKTSPFPVILSFENHVDSSKQQAKMAEYCRNIFGDALLIDPLEKYPLQAGVQLPSPQELMGKILVKNKKRHKVAAKGPEGSVKRRVSEQTSNTYSDSSSVCESSATEEEEPSDPKKSDEGTASSEVNATEEMSTLVNYVEPVKFKSFDVAKKRNKYYEMSSFVETKALEQLTKSPMEFVEYNKKQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTLDLPMQLNVGIFEYNRRSGFLLKPEFMRRTDKHFDPFTENIVDGIVANTVKIKIISGQFLSEKRVGIYVEVDMFGLPVDTKRK-FRTKTSQGNSFNPVWDEEPFIFPKVVLPTLASLRIAVFEEGGKFVGHRILPVSAIRPGYHYICLRNELNQPVCLPALLVYTEVNDYIPDDHQGYANALTNPIKHINLMDQRAKQL 765
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Xenopus
Match: PLCB3 (phospholipase C beta 3 [Source:NCBI gene;Acc:100487267]) HSP 1 Score: 520.776 bits (1340), Expect = 1.513e-164 Identity = 261/575 (45.39%), Postives = 373/575 (64.87%), Query Frame = 3
Query: 759 EFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE--------------------------------XXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEI-IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQL 2381
+ +F+NQ QRD RLNEIL+P + D+ ++LI KYE + + +S +G +++ +EN+I+ +L+ DMNQP + YFINSSHNTYLT QLTG SS E YRQVLL+G RCIELD W G+ ++EP +THGFTM T +P ++VI+AI ESAFK S FPVILSFENH +SKQ + + + +L +PLE PL+ GV LPSP+ + +++ E + +D + +G +E+ ++S++VNYVEP F+SF+ AK++N +E S E A++ L ++P+E V +NK+QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ+LDL MQLN+G+FE R G+LLKP MR +D+ FDPF E+ VDGIVAN++K+ + SG F+S+K V Y+E++MFGLP DT RK ++ +++ P + + F K++ P +A LRI E KFVG LPV IRPGY +I L+NE +Q + +P++L+Y E++DY+P D + +A AL+NPI ++ ++RA+QL
Sbjct: 151 QLMDFLNQKQRDPRLNEILFPPLKRDQVRQLIEKYEPNRQFLERDQMSMEGFSRFLGGDENSIVPPEILDLSDDMNQPLSSYFINSSHNTYLTAGQLTGNSSVETYRQVLLTGCRCIELDCWKGRPQDEEPFITHGFTMTTEIPFKEVIEAIAESAFKTSPFPVILSFENHVDSSKQQAKMAEYCRNIFGDALLIDPLEKYPLQAGVQLPSPQELMGKILVKNKKRHKVAAKGPEGSVKRRVSEQTSNTYSDSSSVCESSATEEEEPSDPKKSDEGTASSEVNATEEMSTLVNYVEPVKFKSFDVAKKRNKYYEMSSFVETKALEQLTKSPMEFVEYNKKQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTLDLPMQLNVGIFEYNRRSGFLLKPEFMRRTDKHFDPFTENIVDGIVANTVKIKIISGQFLSEKRVGIYVEVDMFGLPVDTKRK-FRTKTSQGNSFNPVWDEEPFIFPKVVLPTLASLRIAVFEEGGKFVGHRILPVSAIRPGYHYICLRNELNQPVCLPALLVYTEVNDYIPDDHQGYANALTNPIKHINLMDQRAKQL 724
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Xenopus
Match: PLCB3 (phospholipase C beta 3 [Source:NCBI gene;Acc:100487267]) HSP 1 Score: 520.776 bits (1340), Expect = 1.644e-164 Identity = 261/575 (45.39%), Postives = 373/575 (64.87%), Query Frame = 3
Query: 759 EFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE--------------------------------XXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEI-IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQL 2381
+ +F+NQ QRD RLNEIL+P + D+ ++LI KYE + + +S +G +++ +EN+I+ +L+ DMNQP + YFINSSHNTYLT QLTG SS E YRQVLL+G RCIELD W G+ ++EP +THGFTM T +P ++VI+AI ESAFK S FPVILSFENH +SKQ + + + +L +PLE PL+ GV LPSP+ + +++ E + +D + +G +E+ ++S++VNYVEP F+SF+ AK++N +E S E A++ L ++P+E V +NK+QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ+LDL MQLN+G+FE R G+LLKP MR +D+ FDPF E+ VDGIVAN++K+ + SG F+S+K V Y+E++MFGLP DT RK ++ +++ P + + F K++ P +A LRI E KFVG LPV IRPGY +I L+NE +Q + +P++L+Y E++DY+P D + +A AL+NPI ++ ++RA+QL
Sbjct: 155 QLMDFLNQKQRDPRLNEILFPPLKRDQVRQLIEKYEPNRQFLERDQMSMEGFSRFLGGDENSIVPPEILDLSDDMNQPLSSYFINSSHNTYLTAGQLTGNSSVETYRQVLLTGCRCIELDCWKGRPQDEEPFITHGFTMTTEIPFKEVIEAIAESAFKTSPFPVILSFENHVDSSKQQAKMAEYCRNIFGDALLIDPLEKYPLQAGVQLPSPQELMGKILVKNKKRHKVAAKGPEGSVKRRVSEQTSNTYSDSSSVCESSATEEEEPSDPKKSDEGTASSEVNATEEMSTLVNYVEPVKFKSFDVAKKRNKYYEMSSFVETKALEQLTKSPMEFVEYNKKQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTLDLPMQLNVGIFEYNRRSGFLLKPEFMRRTDKHFDPFTENIVDGIVANTVKIKIISGQFLSEKRVGIYVEVDMFGLPVDTKRK-FRTKTSQGNSFNPVWDEEPFIFPKVVLPTLASLRIAVFEEGGKFVGHRILPVSAIRPGYHYICLRNELNQPVCLPALLVYTEVNDYIPDDHQGYANALTNPIKHINLMDQRAKQL 728
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Xenopus
Match: PLCB3 (phospholipase C beta 3 [Source:NCBI gene;Acc:100487267]) HSP 1 Score: 520.39 bits (1339), Expect = 2.308e-164 Identity = 261/575 (45.39%), Postives = 373/575 (64.87%), Query Frame = 3
Query: 759 EFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE--------------------------------XXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEI-IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQL 2381
+ +F+NQ QRD RLNEIL+P + D+ ++LI KYE + + +S +G +++ +EN+I+ +L+ DMNQP + YFINSSHNTYLT QLTG SS E YRQVLL+G RCIELD W G+ ++EP +THGFTM T +P ++VI+AI ESAFK S FPVILSFENH +SKQ + + + +L +PLE PL+ GV LPSP+ + +++ E + +D + +G +E+ ++S++VNYVEP F+SF+ AK++N +E S E A++ L ++P+E V +NK+QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ+LDL MQLN+G+FE R G+LLKP MR +D+ FDPF E+ VDGIVAN++K+ + SG F+S+K V Y+E++MFGLP DT RK ++ +++ P + + F K++ P +A LRI E KFVG LPV IRPGY +I L+NE +Q + +P++L+Y E++DY+P D + +A AL+NPI ++ ++RA+QL
Sbjct: 157 QLMDFLNQKQRDPRLNEILFPPLKRDQVRQLIEKYEPNRQFLERDQMSMEGFSRFLGGDENSIVPPEILDLSDDMNQPLSSYFINSSHNTYLTAGQLTGNSSVETYRQVLLTGCRCIELDCWKGRPQDEEPFITHGFTMTTEIPFKEVIEAIAESAFKTSPFPVILSFENHVDSSKQQAKMAEYCRNIFGDALLIDPLEKYPLQAGVQLPSPQELMGKILVKNKKRHKVAAKGPEGSVKRRVSEQTSNTYSDSSSVCESSATEEEEPSDPKKSDEGTASSEVNATEEMSTLVNYVEPVKFKSFDVAKKRNKYYEMSSFVETKALEQLTKSPMEFVEYNKKQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTLDLPMQLNVGIFEYNRRSGFLLKPEFMRRTDKHFDPFTENIVDGIVANTVKIKIISGQFLSEKRVGIYVEVDMFGLPVDTKRK-FRTKTSQGNSFNPVWDEEPFIFPKVVLPTLASLRIAVFEEGGKFVGHRILPVSAIRPGYHYICLRNELNQPVCLPALLVYTEVNDYIPDDHQGYANALTNPIKHINLMDQRAKQL 730
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Mouse
Match: Plcb1 (phospholipase C, beta 1 [Source:MGI Symbol;Acc:MGI:97613]) HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0 Identity = 333/833 (39.98%), Postives = 490/833 (58.82%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHEKS 2456
K VKW++ + T + + + D FFF W TE++++SL++D R GK+AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT + + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G +S G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI A E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + +E E + + +P E S
Sbjct: 27 KFVKWDD--DSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDV--GNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLE----DEEEVKKEADPGETS 848
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Mouse
Match: Plcb1 (phospholipase C, beta 1 [Source:MGI Symbol;Acc:MGI:97613]) HSP 1 Score: 584.719 bits (1506), Expect = 0.000e+0 Identity = 333/833 (39.98%), Postives = 488/833 (58.58%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHEKS 2456
K VKW++ + I+ D FFF W TE++++SL++D R GK+AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT + + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G +S G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI A E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + +E E + + +P E S
Sbjct: 27 KFVKWDDDSTIVTPIILRT--DPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDV--GNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLE----DEEEVKKEADPGETS 848
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Mouse
Match: Plcb1 (phospholipase C, beta 1 [Source:MGI Symbol;Acc:MGI:97613]) HSP 1 Score: 584.719 bits (1506), Expect = 0.000e+0 Identity = 333/833 (39.98%), Postives = 488/833 (58.58%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHEKS 2456
K VKW++ + I+ D FFF W TE++++SL++D R GK+AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT + + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G +S G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI A E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + +E E + + +P E S
Sbjct: 27 KFVKWDDDSTIVTPIILRT--DPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDV--GNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLE----DEEEVKKEADPGETS 848
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Mouse
Match: Plcb3 (phospholipase C, beta 3 [Source:MGI Symbol;Acc:MGI:104778]) HSP 1 Score: 330.872 bits (847), Expect = 9.687e-95 Identity = 164/336 (48.81%), Postives = 225/336 (66.96%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHEKSXXXXXXXXXXXXXXXXPLNESP 2522
++S++VNYVEP F+SFE A+++N FE S E A++ L ++P+E V +NK+QLSRIYPKGTRVDSSN++PQLFWN GCQLVALNFQ+LDL MQLN G+FE R GYLLKP MR D+ FDPF E VDGIVAN+L++ + SG F+SDK V Y+E++MFGLP DT R++Y+ R++ P + + F K++ P +A LRI A E KFVG LPV IR GY ++ L+NE++Q L +P++LIY E DY+P D +D+A AL NPI +S ++RA+QL L E E ++ E ++++ S + PL+ SP
Sbjct: 590 EMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQLTKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYLLKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDT-RRKYRTRTSQGNSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALLIYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALI------GESEAQASTETYQETPCQQPGSQLPSNPTPNPLDASP 918 HSP 2 Score: 274.633 bits (701), Expect = 1.293e-75 Identity = 166/441 (37.64%), Postives = 257/441 (58.28%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL--PDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE 1394
K +KW+E E ++ ++V + VD + FF W + ++IS IRD R G+YA+ PKDPKI ++ L E+ +++ G D VN ++ NF++ K + ++ L ++ + SRN L+K + K+ L++ + + + K FS +K E ++ + ++S++I+ +F E F KL L+ ++ I +I + + + +FINQ QRD RLNE+LYP + + +++ LI KYE++ + +S +G +Y+ EEN IL +L+ DM QP + YFINSSHNTYLT QL G SS E+YRQ LL G RC+ELD+W G+ E+EP +THGFTM T VPLRDV++AI E+AFK S +PVILSFENH ++KQ + + + +L +PL+ PL G+PLPSP+
Sbjct: 27 KFIKWDE--EASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIRDTRTGRYARLPKDPKIREVL-GFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAMNILAQNASRN----TFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRSESIRPDEFPLEIFERFLNKLCLRPDIDKILLEIGAKGK---PYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYETNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMYRQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAKQQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQ 457
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Mouse
Match: Plcb4 (phospholipase C, beta 4 [Source:MGI Symbol;Acc:MGI:107464]) HSP 1 Score: 300.827 bits (769), Expect = 1.779e-86 Identity = 137/299 (45.82%), Postives = 196/299 (65.55%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEIL 2390
T I P +S+M+NY +P F+ F A+E+N+ + S E + L + +E VN+NKRQ+SRIYPKG RVDSSN++PQ+FWNAGCQ+V+LN+Q+ DL MQLN G FE CGYLLKP MR DR FDPF E+ VDG++A + + + SG F+SDK + Y+E++M+GLP DT+RK ++ R GL P + + + F+K+I P +A LRI +N K +GQ LP+D ++ GYR I+L+NE ++ L +P++ + YVP D ALS+P FLS E+RA Q+ +
Sbjct: 569 ATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAM 867 HSP 2 Score: 286.574 bits (732), Expect = 1.939e-81 Identity = 167/418 (39.95%), Postives = 247/418 (59.09%), Query Frame = 3
Query: 153 VDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
VDE FF +WK+ +++E SLI I + A PKDPKIL A+ +L R + +C+GTD VN+ + V++ E + + + S I ++ N + LKK W K+ T + + +T+ F+ K ++ + L++ SG + + FT+E+F + +K+ + +E ++ KI D + + + +F+N+ QRD RLNEIL+P ++ ++I YE D L + GLISS G +Y+ S+EN + EL Q+M+ P HYFI+SSHNTYLTG Q GKSS E+YRQVLL+G RC+ELD WDGKG + EPI+THG MCT + +DVI AI E+AF S++PVILSFENHC+ Q + ++ + +LK+ LES+PLEPG PLPSP
Sbjct: 41 VDEFGFFLTWKSEGKEGQVLECSLINSI---RQAAIPKDPKILAALEAVGKSENDLEGRILCVCSGTDLVNIGFTYMVAENPEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFLTNTTGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPAA-FTYEKFYELTQKICPRTDIEDLFKKINGDKTD---YLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEELKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRPLPSP 451
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. UniProt/SwissProt
Match: sp|P10687|PLCB1_RAT (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS=Rattus norvegicus OX=10116 GN=Plcb1 PE=1 SV=1) HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0 Identity = 334/833 (40.10%), Postives = 491/833 (58.94%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHEKS 2456
K VKW++ + T + + + D FFF W TE++++SL++D R GK+AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT + + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G +S G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI A E KF+G LPV IRPGY +I L+NE +Q LM+P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + +E E + + +P E S
Sbjct: 27 KFVKWDD--DSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDV--GNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFETSKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLE----DEEEVKKEADPGETS 848
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. UniProt/SwissProt
Match: sp|P10894|PLCB1_BOVIN (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS=Bos taurus OX=9913 GN=PLCB1 PE=1 SV=1) HSP 1 Score: 587.03 bits (1512), Expect = 0.000e+0 Identity = 334/831 (40.19%), Postives = 487/831 (58.60%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
K VKWE+ + T + + + D FFF W TE++++SL++D R GK+AK PKDPK+ +L + L R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPL+ PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q LM+P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 27 KFVKWED--DSTVVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDV--GNIGRLEHRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLAKKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLDKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNRSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPIWEEEPIVFKKVVLPSLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPALFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 850
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. UniProt/SwissProt
Match: sp|Q9Z1B3|PLCB1_MOUSE (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS=Mus musculus OX=10090 GN=Plcb1 PE=1 SV=2) HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0 Identity = 333/833 (39.98%), Postives = 490/833 (58.82%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHEKS 2456
K VKW++ + T + + + D FFF W TE++++SL++D R GK+AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT + + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G +S G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI A E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + +E E + + +P E S
Sbjct: 27 KFVKWDD--DSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDV--GNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLE----DEEEVKKEADPGETS 848
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. UniProt/SwissProt
Match: sp|Q9NQ66|PLCB1_HUMAN (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 OS=Homo sapiens OX=9606 GN=PLCB1 PE=1 SV=1) HSP 1 Score: 584.334 bits (1505), Expect = 0.000e+0 Identity = 332/831 (39.95%), Postives = 487/831 (58.60%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTE------------------------IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
K VKW++ + T + + + D FFF W TE++++SL++D R G++AK PKDPK+ +L + L +R I++ G D VN+S N V+ +E + + + S ++N L+K + K+ L++ I + + + FS ++ E +A + S++ +I + DFT E + V L + ++ I+S+ S + + +FIN QRD RLNEILYP + ++ + LI KYE + L++ G IS G ++Y+ EEN ++ +L +DM+QP +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP++LSFENH S KQ + + + +L EPLE PLE GVPLPSP + GK + + ++ S + + G G+ TE + ++S++VNY++P FESFE +K++N SFE S E ++ L ++PVE V +NK QLSRIYPKGTRVDSSN++PQLFWNAGCQ+VALNFQ++DL MQ+N+G++E + GY LKP MR D+ FDPF E VDGIVAN+L + + SG F+SDK V Y+E++MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G LPV IRPGY +I L+NE +Q L +P++ +Y E+ DYVP D ALSNPI +++ E+RA+QL LT + E ++E + P E
Sbjct: 27 KFVKWDD--DSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGRHAKAPKDPKLRELLDV--GNIGRLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGA---KSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLARKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTMDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPTLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLTLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSE 850
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. UniProt/SwissProt
Match: sp|Q00722|PLCB2_HUMAN (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 OS=Homo sapiens OX=9606 GN=PLCB2 PE=1 SV=2) HSP 1 Score: 515.383 bits (1326), Expect = 6.102e-162 Identity = 309/823 (37.55%), Postives = 470/823 (57.11%), Query Frame = 3
Query: 90 EKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTA-LSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINMLTKYFSQNKDDREKLEDAXXX---XXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTNDSNEQGDG------------------------------------------------DKGTEII----IPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIR-STTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLE 2384
E+ +KW++ E T S V + VD ++ W + E ++I+ IRD R GK+AK PK K+ D+ D L + +++ +G D V+L+++NFVS + + +++ +K P + N L KI K+ ++L +I + +F DR+++E A+++ K+ I DF + L + ++ I++ H + + FINQ QRD RLN +L+P D+ + LI KYE + +Q G +S +G++ ++ EN++L Q L DM QP NHYFINSSHNTYLT Q +G SSAE+YRQVLLSG RC+ELD W GK ++EPI+THGFTM T + ++ I+AI ESAFK S +P+ILSFENH S +Q + + + +L EPLE PL+PGVPLPSPE L+GKILIKNKK T S+ D+ + +G D+GT + ++SS+VNY++PT F SFE + +KN S+ S E+ A L++ V+ V++NKRQ+SRIYPKGTR+DSSN++PQ+FWNAGCQ+VALNFQ++DL MQ N+ +FE + GYLLK MR D+ F+PF +D +VA +L + + SG F+S++ V Y+E+E+FGLP D ++RY+ + S + + P + + F+KI+ P++A LR+ E KF+G +P++ + GY + L +ES+ L MP++ I+ E+ DY+P D +AL+NPI F S + ++ +L+
Sbjct: 21 ERFIKWDD--ETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLL--KTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIP-VKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSY---HAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDP-KRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLK 834
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. TrEMBL
Match: V4A0H0 (Phosphoinositide phospholipase C OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_123959 PE=4 SV=1) HSP 1 Score: 642.884 bits (1657), Expect = 0.000e+0 Identity = 344/793 (43.38%), Postives = 487/793 (61.41%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL-PD-TLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXX---XDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLP------------------SPEXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDF 2402
K +KW++ N V++ VD WK E ++ISLIRD R G+Y++ PKD K+ D T + + L ++ +S+C GTD VN W NFVS +K+ + ++ N L +L+K++ + L PD + + + K +QN+DD+ ++E+A+ ++G S DFTFE F Y L + ++ ++ +I + + +F F+N QRD RLNEILYP + +++ELI +E+ +++ G +S +G +K++ SE+NN++ +L++DMNQP HYFINSSHNTYLTGHQLTGKSS EIYRQ LL G RC+ELD WDG+G ++EPI+THGFTMCT + +DVI+AI ESAFK S++PVILSFENHCT KQ + + + + +L +E PLEP V LP + + ++T + E+G E + ++S +VNY++P F SFE +++K +E S E A L E PV+ VN+NKRQLSRIYPKGTRVDSSNFLPQ+FWNAGCQLVALNFQ+LDL MQLNLG+FE RCGY+LKP MR DR FDPF ES VDGIVA ++K+ + SG F+SDK V Y+E++M+GLP DTVRK+++ + G+ P + + F+K++ P +A LRI E + +G LPV+ +RPGYR I L+NES+Q L++ ++ ++ I DYVP M +FA AL NPIA+ S+ E+ AQQL LTEDF
Sbjct: 27 KFIKWDDGS--ANGMPVSLRVDSKGHIIFWKDQNKEMEYLDISLIRDTRTGRYSRIPKDAKLRDTLT-IGPGDISLEDKTVSVCFGTDMVNTDWINFVSDSKDTAQEWAKELLEYSTNLLVLNSSALTYLEKVYTRFIKVLGPDGKMPVKNIVKMVAQNRDDKRRVENALGAAGFASGKNDSLDPKDFTFEHFFNFYRHLVGRTEVDKVFDEIGAKKK---PYLTVDQFQTFLNNQQRDPRLNEILYPFYSKVQAQELIEMFENKHGMAEKGHLSQEGFLKFLMSEDNNLIPPEKLDLSEDMNQPLAHYFINSSHNTYLTGHQLTGKSSIEIYRQSLLCGCRCVELDCWDGRGADEEPIITHGFTMCTDILFKDVIEAIAESAFKTSEYPVILSFENHCTPKQQAKMANYCRSIFGDMLLISTIEDYPLEPDVTLPPLSCLKRKILIKNRKKHFHRGSDDDDDAVAGDVLSEEEKTRRQREKREKGTAGSEAEAAM-EMSLLVNYIQPVHFHSFEASEKKGRCYEISSFVETQATNLLKEYPVDFVNYNKRQLSRIYPKGTRVDSSNFLPQVFWNAGCQLVALNFQTLDLAMQLNLGIFEYNGRCGYILKPDFMRRKDRHFDPFAESTVDGIVAGTVKVRVISGQFLSDKKVGTYVELDMYGLPTDTVRKKFRTKVVPANGINPVYDEDPFVFKKVVLPNLATLRIAVYEETGRLIGHRILPVEGLRPGYRHIPLRNESNQPLLLQTLFVHLSIMDYVPDSMAEFAEALINPIAYQSKMEKHAQQLATLTEDF 812
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. TrEMBL
Match: E9FW06 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase OS=Daphnia pulex OX=6669 GN=Plcb2 PE=4 SV=1) HSP 1 Score: 627.861 bits (1618), Expect = 0.000e+0 Identity = 336/828 (40.58%), Postives = 494/828 (59.66%), Query Frame = 3
Query: 54 KGMDISSNDILTEKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL--PDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXX----DFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP----------------------EXXXXXXXXXXXXXXXXXETDSTNDSN---------EQGDGDKGTEI----IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEAN 2414
K + + + I EK VKW+E L V + VD + +F W T+I+++ IRD R G YAK PKD K+ D T + + L ++ +++ G DFVN+S++NF S KE + D ++ + N L+K ++ L+ + + + + F+Q++DDR ++E A S + + F F+ F Y+KL + +E I+ ++ + I +F F+N +QRD RLNEILYP D++++L +YE + + GL+S++G ++++ SE+N+I+ ++L+ DM QP HYF+NSSHNTYLTGHQ TGKSS EIYRQ LL G RC+ELD W+GK ++PI+ HG+T+ VP +DVI+AI ESAFK S+FPV+LSFENHC KQ + +E +L + LES+PLEPGVPLPSP + + + + + + E +S+ SN E+ DKGT +IS++VNYV+P F SFE A+ +N S+E S E + L E+P+E VN+NKRQ+SR+YP+GTRVDSSNF+P +FWNAGCQLVALNFQ+LDL MQLNLG+FE+ R GYLLKP MR DR FDPF ES VDGI+A + + + SG F+SDK V Y+E++MFGLPADTVRKR++ ++ G+ P + + F K++ P +A LRI ++ + +G LPV + PGYR + L+N+ Q L + ++ + + DY+P + DFA AL+NPI + SE E+R++QL IL + +E +
Sbjct: 14 KHVSVPPSMIAGEKFVKWDEDSSLVTA--VTLRVDPYGYFLYWTDQNEETQILDMCTIRDTRTGHYAKVPKDSKMRDQVTMGASQD-SLEDKTLTVSYGPDFVNVSFFNFCSSKKETAKQWTDDLLKMANNLLALNGSVHYFLRKAHVRLTLQADKGGKIAVKNIVRTFAQHRDDRRRVEKAFIESTGLASGKDDAIPLSQFGFDDFYRFYKKLTGRQEVEKIFEELF----GTKLHITVEQFVEFLNSYQRDPRLNEILYPYANPDRARDLFHQYEPNAPNAPKGLLSAEGFLRFLMSEDNSIVSADKFDLSHDMEQPLCHYFVNSSHNTYLTGHQFTGKSSVEIYRQTLLGGCRCVELDFWNGKN--EDPIIYHGYTLVPEVPAKDVIEAIAESAFKTSEFPVVLSFENHCNPKQQAKIAQYCREYFGEMMLDQALESHPLEPGVPLPSPAQLKRKIIIKNKKKHQHHRTLKDHRQDQSSLTLRLFLSDDEDESSTLSNLSPEEIRLREKAQKDKGTAGKETEAGAEISALVNYVQPIHFHSFEHAERRNRSYEMSSFVETQSTALLKEHPIEFVNYNKRQISRVYPRGTRVDSSNFMPHVFWNAGCQLVALNFQTLDLAMQLNLGIFEMNGRTGYLLKPQFMRRMDRKFDPFAESTVDGIIAGKVSVQVISGQFLSDKRVSTYVEVDMFGLPADTVRKRFRTKTVLNNGINPIYDQEPFVFNKVVLPDLACLRIAVYEDSGRLLGHRVLPVTGLCPGYRHLGLRNDGGQPLNLATLFVNIMVKDYIPDGLSDFANALANPIKYQSELEKRSKQLSILKQPSLEGD 832
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. TrEMBL
Match: R7UBY3 (Phosphoinositide phospholipase C OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_170497 PE=4 SV=1) HSP 1 Score: 626.32 bits (1614), Expect = 0.000e+0 Identity = 350/832 (42.07%), Postives = 498/832 (59.86%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKIC--LELPDTLEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQ---INSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQN------GLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE----------------------XXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEIII---------PQISS------------------MVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
K ++WEE + T + V + VD + + WK + ++ISLIRD R GKYAK PKDPK+ D + S +V L ++ +++C GTD VNL++ NF + ++E + D + + N + L K + K+ + + + + K F+ +KDDR+K+E +A KS I S F+FE F+ +Y L + +E I+ HE K+ + + +F FIN QRD+RLNEILYP +++ LI+++E + ++ G +S +G ++Y+ SEEN ++ +L +DM QP +HYFINSSHNTYLTGHQ TGKSS EIYRQVLL+G RC+ELD WDGKG ++EPI+THG+TMCT + ++VI+AI ESAFK SD+PVILSFENHC+ KQ + + L +L +PLE++PL+PGV +PSP K +K + ++ S E G DK E I P +SS +VNY++P F SFE A ++ S+EC S E A L E PVE VNFNKRQLSRIYPKGTRVDSSN++PQ+FWNAGCQLVALN+Q+LDL MQLNLG+FE R G++LKP MR D++FDPF ES VDGIVA ++++ + SG F++D+ V +E++M+GLPADTVRK++K + G+ P + + F+K++ P +A LR A EN + +G LPV+ IRPGYR I+L+NE +Q + ++ +Y DYVP + +FA A+ NPIA+ S+ E+ A QL +L ED
Sbjct: 27 KFIRWEE--DSTVGTPVTLKVDSNGYILYWKDQVKEMDSLDISLIRDTRTGKYAKVPKDPKMRDSLSMGSADVA-LEDKTLTVCYGTDLVNLNFINFAANSREVAKEWCDALFQFATNLLASNSAPMTFLLKCYTKLVTIVNTEGKIPVRNVVKMFATHKDDRKKVEKALEAARLPYGKSDMIHPSKFSFEDFMALYRHLCGRQEVEKIF------HELGAKKKPYLTAEQFVEFINSTQRDRRLNEILYPYCDVKRAQALINQFEPNQNMASKDPQVPKGHLSLEGFMRYLLSEENALIPPEVLDLNEDMEQPLSHYFINSSHNTYLTGHQFTGKSSIEIYRQVLLTGCRCVELDCWDGKGADEEPIITHGYTMCTEIVFKEVIEAISESAFKVSDYPVILSFENHCSPKQQAKMAQHCRNILGEALLTDPLETHPLDPGVCVPSPALLKGRIIVKNKKRHIHKSKAKTVRKPVSRQNSKSAVVEGDAEAAESSGEGGCRDKNDEDDIDGEKAGDDDPDLSSESDLETGQEAEAGMEMSALVNYIQPARFHSFEYADKRGRSYECNSFVETQATALLKEFPVEFVNFNKRQLSRIYPKGTRVDSSNYMPQIFWNAGCQLVALNYQTLDLPMQLNLGIFEYNGRSGFILKPDFMRRKDKEFDPFAESTVDGIVAGTVEVKVISGQFLTDRKVGTCVEVDMYGLPADTVRKKFKTKVIPGNGVNPLWDEDAFIFRKVVLPNLATLRFCAQDENGRLLGHRILPVEGIRPGYRHISLRNELNQPSGIATLFVYIRAGDYVPDALAEFAAAIENPIAYQSKLEKHALQLAVLEED 849
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. TrEMBL
Match: A0A482W360 (Phosphoinositide phospholipase C (Fragment) OS=Asbolus verrucosus OX=1661398 GN=BDFB_002730 PE=4 SV=1) HSP 1 Score: 621.698 bits (1602), Expect = 0.000e+0 Identity = 332/799 (41.55%), Postives = 479/799 (59.95%), Query Frame = 3
Query: 141 VNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT--LEINMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPG------VPLPSPEXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEII-------------------------IPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEE 2429
V + VD++ F+ W ++++I+ +RD R G+YAK PKDPK+ + T S + +E E+ +++C DFVN+++ NF KE D ++S N ++L K + K+ L T + I + K F+Q+KDD++++E K+ I F FE F Y+ L + +E ++ IA + + + + +F +F+N+ QRD RLNEIL+P ++K++I++YE + +Q GL+S G ++Y+ SE+NNI+ S YEL +M+QP HYFINSSHNTYLTGHQLTG+SS E+YRQ LL+G RC+ELD W+G+ +EPI+ HG+T + ++V++AI ESAFK SDFPV+LSFENHC +Q + + +E +L+ PLE LEPG +P P + DST +E D+ E + +IS++VNYV+P F SFE AK+KN S+E S E A L E PV+ VN+NK QLSR+YP GTR DSSNF+PQ+FWNAGCQLVALNFQ+LDL MQLNLG+FE RCGYLLKP MR +DR F PF ES VDGI+A ++K+ + SG F++DK V Y+E+EM+GLPADTVRKR++ + G+ P + + F+K++ P +A +RI A E+ +F+G LPV + PGYR + L+ E Q L + ++ + + DYVP + DFA AL+NPI + S+ E+RAQQL +LT+D A EE+
Sbjct: 8 VTLRVDKYGFYLYWVDQNHEMDLLDIATVRDTRTGRYAKIPKDPKLRQIVTMGSQDTLE--EKTVTVCCANDFVNINFINFCCTRKEIARLWTDALMSLAYNLNQINGTTNLYLLKAYTKLILITDKTGKIPIKNVIKMFAQSKDDKKRIEMVLHMTGLPNGKNDAISPEKFQFEDFFNFYKNLTQRTEVEKVFYDIAGLKK---QHMTAAQFVDFLNKTQRDPRLNEILHPYANTTRAKDIIAQYEPNKYNAQKGLLSIDGFLRYLMSEDNNIIASSKYELCDEMDQPLAHYFINSSHNTYLTGHQLTGRSSIEMYRQCLLAGCRCVELDFWNGR---EEPIIVHGYTFVPEISAKEVLEAIAESAFKTSDFPVVLSFENHCNPRQQAKIANYCKEIFGEMLLEAPLEMYKLEPGASSSPEIPTSEPTGNGDVPHHAPPLQTRQGSKDSTGTEDEDSSTDEDGETLETDNPTEAPNEGNDHKSAAASETEAGAEISALVNYVQPVHFSSFENAKKKNRSYEMSSFDERQATSLLKEYPVQFVNYNKHQLSRVYPAGTRFDSSNFMPQVFWNAGCQLVALNFQTLDLAMQLNLGIFEYNFRCGYLLKPEFMRRTDRRFYPFAESTVDGIIAGTVKVTVISGQFLTDKRVGTYVEVEMYGLPADTVRKRFRTKVVPYNGINPLYDEEPFVFKKVVLPDLASIRIAAYEESGRFIGHRILPVVGLCPGYRHVNLRTEIGQPLPLATIFVQVVVKDYVPDGLSDFAEALANPIKYQSDLEKRAQQLAVLTDDMEPAEGGEEQ 798
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. TrEMBL
Match: T2M532 (Phosphoinositide phospholipase C (Fragment) OS=Hydra vulgaris OX=6087 GN=PLCB1 PE=2 SV=1) HSP 1 Score: 593.578 bits (1529), Expect = 0.000e+0 Identity = 346/810 (42.72%), Postives = 492/810 (60.74%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTA--TGATXXXXXXXXXXXXXGKYAKFPKDPKI-----LDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEI--NMLTKYFSQNKDDREKLE---DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXX-XXXXETDSTN------DSNEQG-------------DGDKGTEIIIP--------------QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLE 2384
+ +KW++ + CSI + VD+ SWK + TE++++ +RD+R G+YAK PK I + + +A E + ER I+I G++FV+L++ NFV+ + + D + N L L++ + +I ++ EI +L K F NK+D+++ ++ + K +TI DFTFERFL Y + + L+ +++++ ++ + + + +F+N QRD RLNEIL+P ++ +++ YE + + G IS GL K++ S + NIL ++ QD+ P HYFINSSHNTYL GHQLTGKSS EIYRQVLL+G RCIELD WDG+ E EP++THG T+CT VP +DVI+AI ESAFK S++PVILSFENHC++KQ + S + + +L + LE PL PGVPLPSP+ LK KILIKNKK+ ET ++N D N G DG+ + ++P ++S +VNY +P F FE+A++ +E S E A+ L E PVE VN+NK Q SRIYPKG+RV S N++PQLFWNAGCQLVALNFQ+LD+ MQLNLG FE+ RCGYLLKP MR DR FDPF+ES VDGI+A ++++ + SG + ++ Y+E+EM+GLPADTVR++Y+ + + GL P + + F K+I P +A+LRI EN K +G LPVD + PGYR I L+NES Q L +P++ + DYVP DFA ALSNP+ +LSEKE+R QQLE
Sbjct: 25 RFLKWDDQD--LACSIYILEVDQLGHVLSWKPEDFSKDTEVLDLVHVRDVRTGQYAKVPKPQDIRARDAIKMYSAFEHESNLMQERTITIVYGSNFVDLTFINFVASSVSEAQEWTDALFKCTNNLLEFNSSPLKCLERFFTRIYVQKNQEGEIPTKILVKAFVNNKEDKKRAYGTLQSVGIASGKKETILVKDFTFERFLAFYNRFVTRHDLDGVFAELGAKNK---PYLTTDQLVDFLNNRQRDPRLNEILFPYYDISGAQAIVNTYEPNKAFADKGYISIDGLTKFLMSSDANILLPGKLQIHQDLTFPVAHYFINSSHNTYLQGHQLTGKSSVEIYRQVLLAGCRCIELDCWDGRTEEQEPVITHGMTLCTEVPFKDVIEAIKESAFKTSEYPVILSFENHCSAKQQKKMASYCLQIFGDLLLNKELEECPLLPGVPLPSPKMLKRKILIKNKKRDIKVSETKNSNLFAQSIDLNISGVMLEISNMEDIPEDGE--LKEVVPKSEEEETPEVEAQQELSELVNYFQPVHFRGFERAEKVKRYYEMSSFSENAAMNLLKEQPVEFVNYNKFQASRIYPKGSRVSSDNYIPQLFWNAGCQLVALNFQTLDVPMQLNLGKFEINGRCGYLLKPEHMRRHDRTFDPFVESTVDGIIAGTVQVRVISGQCLLERRSGTYVEVEMYGLPADTVRRKYRTKVVSNNGLNPVYDEEPFKF-KVILPHLAELRISVYEENGKMIGHRVLPVDGLSPGYRHIKLRNESYQPLCLPTLFVDIVTKDYVPESFSDFADALSNPMKYLSEKEKRTQQLE 826
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Cavefish
Match: PLCB4 (phospholipase C beta 4 [Source:NCBI gene;Acc:103022380]) HSP 1 Score: 563.533 bits (1451), Expect = 0.000e+0 Identity = 316/781 (40.46%), Postives = 448/781 (57.36%), Query Frame = 3
Query: 153 VDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICL--ELPDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXD---FTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP--------------------------EXXXXXXXXXXXXXXXXXETDSTNDSNEQGDG----DKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEIL 2390
VDE FF +WK+ +++E SLI +R+G PKDPKIL A +L I +C+G D VNLS+ V+ + TK + + S I K+ N C + LKK W ++ + + + +T+ F K ++ + A+ G S + D FTF+ F + +K+ + +E ++ KI D + + + +F+N+ QRD RLNEIL+P + ++I KYE D L + G +SS G +Y+ S+EN + ++ QDM+QP HYFI+SSHNTYLTG Q GKSS E+YRQVLLSG RC+ELD WDGKG + EPI+THG MCT + +DVI AI E+AF S++PVILSFENHC+ Q + ++ +LK+PLE P+EP PLPSP ++ + + E D ND+ +Q + T I P +S+MVNY +P F+SFE A+EKN+ S E + L N +E VN+NKRQ+SRIYPKG RVDSSN++PQ+FWNAGCQ+V+LNFQ+ DL MQLN G FE CGYL KP MR SDR FDPF E+ VDG++A + + + SG F+SDK + Y+E++M+GLP DT+RK ++ R GL P + + F+K+I P +A LRI +N K +GQ LP+D ++ GYR I+L+NE ++ L +P++ + YVP ALS+P FLS E+RA Q+ L
Sbjct: 41 VDEFGFFLTWKSEGKEGQVLECSLINSVRVGA---VPKDPKILSSLEAAGKNEADLEGCIICVCSGPDLVNLSFMYMVADNAD--TKWLEGLKSVIHNFKANNVCPMTCLKKHWMRLSFLTNVNGKIPVRGITRTFGSGKTEKGIFQ-ALKELGLPSGKNDEIDHSMFTFDVFYALTQKICPRTDIEELFKKINGDKTD---YLTVDQLVSFLNENQRDPRLNEILFPFYDPKRVMQIIEKYERDEDLKKKGRMSSDGFCRYLMSDENAPVFLDRLDMCQDMDQPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLSGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKPQQYKMAKYCEDIFGELLLKQPLEGFPIEPARPLPSPNDLKRKILIKNKRLKPEVEQKQLESFKRHMEAGEMSVQGGDDENEEDMENDAEQQLIASYKYEGATTNIHPYLSAMVNYAQPVKFQSFEIAEEKNIHHNMSSFNESVGLGYLKTNAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNFQTPDLAMQLNQGKFEYNGACGYLQKPDFMRRSDRMFDPFSETPVDGVIAATCSVQVFSGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPAYNEEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTVFCNIVLKTYVPDGFGAIVDALSDPKKFLSIAEKRADQMRAL 812
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Cavefish
Match: PLCB1 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 [Source:NCBI gene;Acc:103021983]) HSP 1 Score: 554.673 bits (1428), Expect = 8.807e-179 Identity = 316/754 (41.91%), Postives = 449/754 (59.55%), Query Frame = 3
Query: 309 VVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXX---XXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTNDSNEQ------------------------------------GDGDKGTEIIIPQ-ISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESD 2435
V ++ R +++ TG D VN+++ NF++ KE+ ++ L + + SR C L+K+ IC L + + + FS DR+++E A+ + S + FT E + V + + ++ I+S+I S + + +FIN QRD RLNEILYP + ++ + L+ KYE D +L + G IS +G +Y+ EEN+I+ + ++DM P +HYFINSSHNTYLT QL G SS E+YRQVLLSG RCIELD W G+ E+EPI+THGFTM + + ++VI+AI ESAFK S FPVILSFENH S KQ + + + +L EPLE PL+ G PLPSP L GKILIKNKK + E + EQ +G G+E + +S++VNYV+P F SFE +K+ N S+ S E A++ L ++PVE V +NK QLSR+YPKGTR+DSSN++PQ+FWNAGCQLVALNFQ++DL MQLNLG+FE + GY LKP MR D+ FDPF ES VDGIVA++L + + SG F+SD+ V Y+E++MFGLP DT RK ++ +++ + P + + I F+K++ P +A LRI +N K +GQ +PV IRPGY +I L+NE +Q+L +P++ +Y E+ DYVP D ALSNPI ++S E+RA QL LT++ E E + + D
Sbjct: 19 VGKMENRILTVVTGPDMVNITYLNFMAFQDEVAKEWAEELFKLASNLLAQNMSRESC----LEKVLKNICY-----LFCSSIFRAFSA---DRKRVETALENCSLPSGRNDFIPEQQFTKEIYRVFLNSICPRKDIDTIFSEIGA---KSRPYLTVEQMTDFINNKQRDPRLNEILYPPLKPEQVQALVEKYEPDDMLCKRGQISVEGFGRYLSGEENSIIPPEKLDQSEDMMFPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCIELDCWKGRTTEEEPIITHGFTMTSEISFKEVIEAIAESAFKTSPFPVILSFENHVDSPKQQAKMAEYCRSIFGDALLTEPLEKYPLDCGSPLPSPMDLLGKILIKNKKNSHKDEGSTKKKLTEQTSNTCSDTSSMCEPSSPSAGTLEDEDDEDDDCKKSMDEGTAGSEAFATEEMSNLVNYVQPVKFHSFEASKKTNRSYHMSSFVETKALEQLTKSPVEFVEYNKLQLSRLYPKGTRMDSSNYMPQVFWNAGCQLVALNFQTIDLPMQLNLGMFEYNGKSGYRLKPEFMRRPDKHFDPFTESTVDGIVAHTLSVKIISGQFLSDRKVGTYVEVDMFGLPVDTKRKAFRTKTSPGNAVNPVWDEEPIVFKKVVLPTLASLRIAVYEDNGKIIGQRIIPVSAIRPGYHYIGLRNEKNQTLTLPAIFVYIEVKDYVPDTFADVIEALSNPIRYISLMEQRASQLAALTQEEGEQEENKPKPD 757
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Cavefish
Match: plcb3 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 [Source:NCBI gene;Acc:103029357]) HSP 1 Score: 556.984 bits (1434), Expect = 2.659e-178 Identity = 332/833 (39.86%), Postives = 493/833 (59.18%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGA-TXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPD--TLEINMLTKYFSQNKDDREKLEDAXXX---XXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHC-TSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTNDS-----------------NEQGDG-------------------DKGTEI----IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDGEPHE 2450
K +KW+E ++V + VD + FF W EI++IS IRD R GK+AK PK + +L E + + +++ G D VN+S+ NF + ++ + + S+N L K + K+ L++ + + + K FS D+ ++E A+ +++ +K DFT+++F L ++ ++ I+ ++ ++ + +FIN+ QRD RLNE+LYP + ++ ++L+ KYES+ + IS KY+ EEN ++ +++ DMNQP +HYFINSSHNTYLT QLTG SS E+YRQVLL+G RCIELD W G+ ++EPI+THGFTM T +P ++VI+AI ESAFK S +PVILSFENH +++Q + + + +L +PLE PL PG LPSP++L GKILIKNKKK +TN N++GDG D+GT ++S++VNY+EP F+SFE A ++N FE S E + SL NP+E V +NK+QLSRIYPKGTRV+SSN++PQLFWN GCQ+VALNFQ+LDL MQLNL +FE GYLLKP MR +D+ FDPF E+ VDGIVAN++K+ + SG F++DK V Y+E++MFGLPADT RK ++ +++ + P + F K++ P +A LR+ EN KF+G LPV +RPGY +I LKN+ +Q L++ S+L+Y E+ DY+P + + +A AL+NPI + ++ R +QL + ED + N+R ++ +GE E
Sbjct: 27 KFIKWDEEPSSGQPTLVTLKVDPYGFFLYWSGGGNMEVEIVDISHIRDTRTGKFAKLPKALTVRELLGFGKGEG-NVEGKLVTVVHGNDLVNISFLNFQAMQEDIAKVWTEELFKLATNILSQNSSRNTFLLKAYTKLTLQVTQDGKIPVKNILKMFS----DKRRVESALEQCGLVANRADGVKLDDFTWDKFQSFLNNLCMRPEIDRIFLELG---SKGKPFLSLDQMLDFINRRQRDSRLNEVLYPPLKREQVRQLMEKYESNPSQLERDQISLMTFSKYLGGEENAVVPPERFDVMDDMNQPLSHYFINSSHNTYLTVGQLTGPSSVEMYRQVLLTGCRCIELDCWKGRPPDEEPIITHGFTMTTEIPFKEVIEAIAESAFKTSPYPVILSFENHVDSTRQQAKMAEYCRSIFGDALLIDPLEKYPLVPGQQLPSPQELMGKILIKNKKKHHHHHHQATNGGSVRRKAGDGAEDQASPLNDRGDGESEEEEEEEPVADPKKHHSDEGTAYSEVNATEEMSTLVNYIEPVKFKSFELAAKRNKYFEMSSFVETKGMDSLKNNPIEFVEYNKKQLSRIYPKGTRVESSNYMPQLFWNVGCQMVALNFQTLDLPMQLNLSVFEYNGHSGYLLKPEFMRRTDKHFDPFTENIVDGIVANTVKIKIVSGQFLTDKKVGVYVEVDMFGLPADTKRK-FRTKTSNNNSMDPVWDDEAFVFSKVVLPTLASLRVALFEENGKFIGHRILPVVALRPGYHYINLKNDLNQPLLLASLLVYTEVMDYIPDEHQGYADALTNPIKHVLDQ--RERQLAVFMEDNNDGNKRVQQDEGEKPE 848
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Cavefish
Match: si:ch1073-140o9.2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 [Source:NCBI gene;Acc:103033987]) HSP 1 Score: 540.806 bits (1392), Expect = 9.611e-174 Identity = 303/746 (40.62%), Postives = 444/746 (59.52%), Query Frame = 3
Query: 327 RCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDT---LEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTND------SNEQGD--------------------------------GDKGTEIIIPQ-ISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESD 2435
+ +++ TG D VN+++ NF++ +E + + + S ++N L+K + ++ L+ P+ + + + + FS ++ E ++ ++ +I D T + + + + + I++ + +N + T+F NF QRD RLNEILYP + ++++ L+ K+E + Q G IS +G +++ S+EN ++ + ++DM P +HYFINSSHNTYLT QL G SS E+YRQVLLSG RC+ELD W G+ E+EP++THGFTM + + ++VI+AI E AFK S FP+ILSFENH S KQ + + + +L EPLE PLE GVPLPSP++L GKIL+KNKK ST SN D G G+E + +S++VNY++PT F SFE +K+ + S++ S E A++ L + PVE V +NK QLSRIYPKGTRVDSSN++PQ+FWNAGCQLVALNFQ++DL MQLNLG++E + GY LKP MR D+ FDPF ES VDGIVAN+L + + SG F++DK V Y+EI+MFGLP DT RK +K +++ + P + + I F+K++ P ++ LRI E KF+G +PV IRPGYR+I L+NE +QSL +P++ +Y E+ DYVP D ALSNPI +++ E+R++QL LT E EEE D
Sbjct: 4 KMLTVVTGPDMVNITYLNFMAFQEEVAKEWAEELFSLASNLLAQNMNREASLEKAYTRLKLQ-PNQEGRIPVKNIFRMFSADRKRVETALESCNLPSGRNDSIPLEDLTCDIYKSFIGNICPRPEINQIFADLGAKGKNYLSMEQMTDFINFK---QRDPRLNEILYPPLRTEQTQLLMEKFEPSNSMIQKGQISVEGFSRFLISDENGVIPPEKLDQSEDMTFPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTSEISFKEVIEAIAECAFKTSPFPIILSFENHVDSPKQQAKMAEYCRSIFGDALLTEPLEKYPLESGVPLPSPQELMGKILVKNKKSHPKPADGSTKKKLSEQASNTNSDSSSVFEPSSPSAGPAGTRDDDDDDDCKKSMDEGTAGSEAFATEEMSTLVNYIQPTKFHSFETSKKVSRSYQMSSFVETKALEQLTKTPVEFVEYNKLQLSRIYPKGTRVDSSNYMPQVFWNAGCQLVALNFQTIDLSMQLNLGMYEYNGKSGYRLKPEFMRRPDKHFDPFTESTVDGIVANTLSIKIISGQFLTDKKVGVYVEIDMFGLPVDTRRKAFKTKTSQNNAINPVWDEEPIVFKKVVLPTLSSLRIAVFEEGGKFIGHRIIPVSAIRPGYRYIGLRNEKNQSLTLPALFVYIEVKDYVPDTFADVIEALSNPIRYVNLMEQRSKQLAALT-----LEEGEEEKD 740
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Cavefish
Match: PLCB4 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4 [Source:NCBI gene;Acc:103033015]) HSP 1 Score: 504.597 bits (1298), Expect = 4.562e-161 Identity = 301/796 (37.81%), Postives = 445/796 (55.90%), Query Frame = 3
Query: 153 VDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICL--ELPDTLEINMLTKYFSQNKDDR---EKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKF-SDFPVILS-FENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXE-------------------TDSTNDSNEQGDGDKGTEI------------------------IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEIL 2390
VD FF +WK+ +++E SLI I + PKDPKIL A +L R I IC+G D VNLS+ V++ + D + S I ++ N C + L+K W ++ + + + +T+ F+ K ++ + L+D SG + + FTFE+F + +K+ + +E+++ K+ + + +N+ + +F+N+ QRD RLNEIL+P + +++++I YE+D L + GL+S G +Y+ S+EN + EL Q+M+QP HYFI+SSHNTYLTG Q G+SS E+YRQVL RC+ELD WDGKG + EPI+THG MCT DV+ +++ F + P S F H +Q L ++ + +L++PL+ PLEPG PLPSP LK KILIKN++ E T+ + D NE+ G T I I P +S+++NY + F FE+A+E N+ S E + L N +E VN+NKRQ+SRIYPKG RVDSSN++PQ+FWNAGCQ+V+LNFQ+ DL MQLN G FE CGYLLKP MR DR FDPF E+ VDG++A + + + SG F+SDK + Y+E++M+GLP DT+RK ++ R GL P + + F+K+I P +A LRI +N K +GQ LP+D ++ GYR I+L+NE ++ L +P++ + YVP ALS+P FLS E+R Q+ +
Sbjct: 41 VDAFGFFITWKSEGKEGQVLECSLINSI---RSTSVPKDPKILSALEATGKTESDLEGRIICICSGADLVNLSFMYIVAQDISIAKQWTDSLRSLIHNSRANNVCPMTCLRKHWMRLSFLTNVNGKIPVRSITRTFASGKTEKVIFQALKDLGLPSGKNDELEPDA-FTFEKFYSLSQKICPRTDIEVLFKKLCGEKSDF---LNTDQLVSFLNENQRDPRLNEILFPFYDSKRARQIIESYETDEKLQKKGLMSCDGFSRYLMSDENAPVFLDRLELYQEMDQPLAHYFISSSHNTYLTGRQFGGRSSVEMYRQVLCLACRCVELDCWDGKGEDQEPIITHGKAMCT-----DVLFKVNKKCRPFLVNLPCPHSSFMFHRRHQQY-KLARYCEDIFGDTLLRQPLDDFPLEPGQPLPSPNDLKRKILIKNRRLKPEVEKKQLEHFRKHMEAGDVNMSTNMSEDENEEDAGCGKTVIACEPGVADEQALIQSYTYVAATTNIHPYLSNIINYAQAVKFLGFEEAEETNIHHNMSSFNESVGLGYLRTNAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNFQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRMFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEEPFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTVFCNIVLKTYVPDGFGAIVDALSDPKKFLSICEKRTDQMRAM 823
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Sea Lamprey
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 335.88 bits (860), Expect = 6.503e-99 Identity = 155/315 (49.21%), Postives = 220/315 (69.84%), Query Frame = 3
Query: 1467 SNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEA 2411
S ++G + I ++S++VNYV+P F +FE A++KN S+E S E ++ L + PVE V +NK QLSRIYPKGTRVDSSN++PQ+FWNAGCQLVALN+Q++DL MQLN+G+FE R GY+LKP MR SD+ FDPF ++ VDGI+AN++ + + SG F+SDK V C++E++MFGLP DT RK YK ++ + P + + F+K++ P +A LR+ A + KF+G +PV IRPGYR I L+NES+Q+L +P++L+Y ++ DYVP D AL+NPI ++S E+RAQQL L +F E
Sbjct: 495 STDEGSCNSSEAIATEEMSNLVNYVQPVKFTTFEAARKKNRSYEMSSFVETKGMEQLTKCPVEFVEYNKCQLSRIYPKGTRVDSSNYMPQVFWNAGCQLVALNYQTMDLAMQLNIGMFEYNRRSGYILKPEFMRRSDKHFDPFSDNTVDGIIANTVSVKVISGQFLSDKKVGCFVEVDMFGLPVDTKRKIYKTKTAPGNSINPVWDEEPFVFKKVVLPTLACLRVAAFEDGGKFLGHRIMPVSAIRPGYRHICLRNESNQALALPAILVYIQVKDYVPDAFADLTEALANPIRYVSLMEQRAQQLAALIGEFDEG 809 HSP 2 Score: 244.202 bits (622), Expect = 3.989e-67 Identity = 135/366 (36.89%), Postives = 215/366 (58.74%), Query Frame = 3
Query: 318 LSERCISICTGTDFVNLSWYNFVSKT----KEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL--PDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE 1394
L ++ +++ G+DFVNL++ NFV+ + KE+ T I L + + SR+ + L K++ K+ L+L + + + + F+ ++ E ++ ++ I ++F + F + + + + ++ I++++ S + +F FIN QRD RLNEILYP + + + +I KYE + L++ G IS G +K++ +EN+ + +L+ DM QP +HYFINSSHNTYL QL G SS E+YRQ LL+G RC+ELD W G+ ++EP++THGFTM T + +DVI+AI + AFK S FPVILSFENH S KQ + + + +L +PLE LE G+PLPSP+
Sbjct: 18 LEQKTVTVVFGSDFVNLTFLNFVAFSEEIAKEWATGIFQLASNLLYHNSSRD----IFLDKLYTKLTLQLNQDSKIPVKNICRLFAADRRRVETALESCGLPNGRNDAIYPAEFGPDVFRLFIKSICPRPEIDGIFAEVG---ARSRPYLTVDQFTEFINNCQRDPRLNEILYPPLKPSQVQLMIEKYEPNASLAKKGHISVGGFLKFLQGDENSAIPAEKIDLSDDMMQPLSHYFINSSHNTYLMAGQLAGSSSVEMYRQALLAGCRCVELDCWKGRTADEEPVITHGFTMTTEILFKDVIEAIKDCAFKTSPFPVILSFENHVDSPKQQAKMAEYCRTIFGDALLMDPLEKYLLESGIPLPSPQ 376
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Sea Lamprey
Match: PLCB4 (phospholipase C beta 4 [Source:HGNC Symbol;Acc:HGNC:9059]) HSP 1 Score: 303.523 bits (776), Expect = 2.063e-91 Identity = 138/299 (46.15%), Postives = 200/299 (66.89%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEIL 2390
GT I P +S++VNY +P F++F A+E+N+ + S E + L +ELVN+NKRQ+SRIYP+G RVDSSN++PQ+FWNAGCQ+V+LNFQ+ DL MQLN G FE CG+LLKP MR DR FDPF E+ VDG++A + + + SG F+SD+ V Y+E++M+GLP DT+RK ++ R GL P + + F+K+I P +A LRI ++ K +GQ LP+D ++ GYR I+L+NE ++ L +P++ + YVP DF ALS+P FLS+ E+RA Q++ +
Sbjct: 17 GTTNIHPYLSALVNYAQPVKFQNFVVAEERNIHYNMSSFNESVGLGYLKTGAIELVNYNKRQMSRIYPRGGRVDSSNYMPQIFWNAGCQMVSLNFQTPDLAMQLNQGKFEYNGFCGFLLKPDFMRRPDRTFDPFSETPVDGVIAGTCSVQVISGQFLSDRQVGTYVEVDMYGLPTDTIRKEFRTRLVPNNGLNPLYNQEPFVFRKVILPDLAVLRIAVFDDSNKLIGQRILPLDGLQTGYRHISLRNEGNKPLSLPTVFCNIVLKTYVPDGFGDFVDALSDPKRFLSQAEKRANQMQAM 315
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Sea Lamprey
Match: PLCB1 (phospholipase C beta 1 [Source:HGNC Symbol;Acc:HGNC:15917]) HSP 1 Score: 317.39 bits (812), Expect = 3.076e-91 Identity = 152/314 (48.41%), Postives = 216/314 (68.79%), Query Frame = 3
Query: 1473 EQGDGDKGTEII----IPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTT-KTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
++ + D+GT I ++S++VNYV+P F+SFE AK++N ++E S E ++ L + PVE V +NK QLSRIYPKGTRVDSSN++PQ+FWNAGCQ+VALN+Q+LDL MQLN+G+FE R GYLLKP MR +D+ FDPF + +DG++AN++ + + SG F+SD+ V Y+E++MFGLP DT RK YK R++ + P + + F K++ P +A LRI E KF+G LPV IRPG+ I L+NES+Q L +P++ ++ ++ DYVP E+F LSNPI + E+RA+QLE LT D
Sbjct: 491 KKANTDEGTASIEAGASEEMSNLVNYVQPIKFDSFEDAKKRNRNYEMSSFVESKGMEQLTKCPVEFVEYNKTQLSRIYPKGTRVDSSNYMPQVFWNAGCQMVALNYQTLDLAMQLNMGIFEYNCRSGYLLKPEFMRRADKQFDPFTQDIIDGVIANTISVKVISGQFLSDRKVSTYVEVDMFGLPVDTKRK-YKTRTSKDNNSINPVWEEDPFVFDKVVLPSLASLRIGVFEEGGKFIGHRILPVSAIRPGFHHICLRNESNQPLSLPAIFVFIQVKDYVPVAFENFISGLSNPIMYQGLMEQRAKQLESLTMD 803 HSP 2 Score: 246.899 bits (629), Expect = 2.088e-67 Identity = 142/367 (38.69%), Postives = 214/367 (58.31%), Query Frame = 3
Query: 312 VELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLE--LPDTLEINMLTKYFSQNKDDREKLEDAXXX---XXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE 1394
V + R I++ G D VN+S+ NF + T+ D + + ++N L K + K+ L+ D + + + + F D++K+E+A+ S ++ I FT+E F KL + ++ I+++ ++ + +F +FIN QRD RLNE+L+P + + + LI K+ES L++ G IS G ++Y+ EEN I+ +L +DM QP +HYFINSSHNTYLTG QL G SS E+YRQ LL+G RC+ELD W G+ +++PI+THGFTM T +P ++VIDAI E+AFK S +PV+LSFENH S KQ + + + +L + L+ PLE GVPLPSPE
Sbjct: 17 VGMESRTITVVHGPDLVNISFLNFTALTESVAKVWTDELFTLATNLLAQNSSRDKFLNKAYTKLKLQSNTEDKVPVRSILRMFPT---DKKKVENALMSCELPSGRNDNIPIETFTYEAFYSFVNKLCPRTDIDSIFAERYGGQASAXMTVE--QFTDFINSKQRDPRLNEVLFPPMKTHQVQALIDKFES-TNLAKKGQISVLGFLRYLNGEENGIVPPEKLDLNEDMTQPLSHYFINSSHNTYLTGGQLKGNSSVEMYRQSLLAGCRCVELDCWKGRTADEDPIITHGFTMTTEIPFKEVIDAIAETAFKTSPYPVVLSFENHVDSPKQQAKMAEYCRLMFGDALLVDVLDKYPLESGVPLPSPE 377
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Sea Lamprey
Match: PLCD4 (phospholipase C delta 4 [Source:HGNC Symbol;Acc:HGNC:9062]) HSP 1 Score: 265.774 bits (678), Expect = 5.055e-77 Identity = 190/584 (32.53%), Postives = 299/584 (51.20%), Query Frame = 3
Query: 654 FLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIY--RQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDS---TNDSNEQGD----------------------GDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLH--SGHFI-----SDKG--VYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTK----FVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEI 2285
F+V Y+ L +D L+ ++S ++ D SV ++ E A F+ FQR++ E+ + + + + ++E + +++ G ++Y+ S + ++ DQ + + DM +P +HYFI+SSHNTYL QL G SS E Y + G RC+ELD WDG+ EP++ HG T+ + + DVI I++ AF+ SD+PVILS ENHC+ Q L +Q L +K+L PL P LPSPE+L+GK+L+K K+ P ++ N +E G+ G K + + ++S+ V Y F F + +++ +++ S E A + L E+ E V N QLSR+YP G R DSSN+ PQ FWN GCQ+VALNFQ+ M L+ G F CGY+LKP + F P E+ GI ++++L + SG + S+KG + + +E+ G+P D RY+ + G PQ+ +SF I P++A LR + +T FV Q + +R GYR I L + + S+ I+ +I
Sbjct: 58 FVVFYKLLTQRDELQALFSSLSGD--GSV--LSRGELAAFLRDFQREEGCEELRF---AEELASSITERHELSNIARSLSVMTLDGFVRYLTSPDGSLWDQRHDSIGHDMTRPLSHYFISSSHNTYLMEDQLRGPSSTEAYISSRAFRKGCRCVELDCWDGQ--NGEPVIYHGHTLTSKILFSDVIRTIEKYAFQVSDYPVILSIENHCSVAQQAVLARHMQSILGDKLLTAPLAG---LPSSQLPSPEQLRGKVLVKGKRLPGPWPREAVPVANGHDESGEVSDEDEAAEMEDESVRNCVRQRGKKCKQNLSMELSNCVVYCRSVQFPGFSRPRDQASAWDISSFTETKARKLLREDGNEFVRHNTLQLSRVYPAGRRTDSSNYNPQEFWNVGCQIVALNFQTAGDEMDLHDGHFRQNGGCGYVLKPAFLCDPTTAFQP--ENPQPGIGLHTVRLTIKVISGQQLPKVPHSNKGSIIDPLVRVEVHGVPLDNA--RYETKYIDNNGFNPQW-NEILSF-NINVPEVALLRFVVEDHDTATPNDFVAQYSIAFSSLRQGYRHIPLLSRDGTRVEPSSLFIHVKI 623
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Sea Lamprey
Match: plcd3b (phospholipase C, delta 3b [Source:ZFIN;Acc:ZDB-GENE-081222-2]) HSP 1 Score: 266.544 bits (680), Expect = 4.521e-76 Identity = 186/586 (31.74%), Postives = 281/586 (47.95%), Query Frame = 3
Query: 636 DFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVP---LPSPE-----------XXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEIIIPQ-----------ISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFI-----SDKG--VYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILAS----TENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEI 2285
D + F+ ++ KL +D + ++ +++ D + + A F+ Q + + ++ +I+++E GL++ G ++Y+ S + ++ DQ + ++ DM QP +HYFI+SSHNTYLT QL G SS E Y + L+ G RC+ELD WD G + EP++ HG+T+ + + RDVI +I AFK S +PVILS ENHC +Q IV R IL E L + PL PG P LPSPE K + IK+ K + +D+ +D E G + +S V Y FE E+A+ E CS E + + V +N + LSR YP GTRV S N+ P WNAGCQ+VALNFQ+ M L GLF CGY+LKP +R DFDP + G L + + SG + S +G + + +E+ G+ DT + + GL P + K FQ + P++A +R + T F+GQ LP +R GYR + L + +SL ++ ++ I
Sbjct: 193 DLVEDEFVQLFRKLTRRDDVATLFKRLSPDGIG----LPAARLAQFLQDEQHEDD---------SEHRAACIIAEHEPLDEGRSKGLMTLDGFVRYLTSPDGSLWDQRHDSVSHDMTQPLSHYFISSSHNTYLTDDQLRGPSSTEAYVKSLMRGCRCVELDCWD--GADGEPVIYHGYTLTSQILFRDVITSIAHEAFKASAYPVILSLENHCNVEQ-----QIVMARHLRSILGERLVTAPL-PGRPPTQLPSPEELKGKFLIKAKKRSKPVNIKSSAKGLGQASDTESDVFEDSKPSTGKHKPLKAEKSKKKEVSKELSDCVIYCSGVHFEGLERAESHQKGNEMCSFSETKTMGLIETQGNAFVRYNMQHLSRTYPSGTRVGSGNYNPHDMWNAGCQIVALNFQTPGKEMDLYDGLFRQNGGCGYVLKPSFLRDPHTDFDPGNPARGTGRHPVKLAITVISGQQLPKERNSRRGSIIDPLVSVEVHGVRGDTAVR--ETHHQENNGLNPVW-KEEFLFQLTV-PELALIRFVVKDYDVTSRNDFIGQFSLPFSCLRQGYRHVHLLSLDGRSLFPATLFVHIRI 753
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Yeast
Match: PLC1 (Phospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation [Source:SGD;Acc:S000006189]) HSP 1 Score: 152.14 bits (383), Expect = 5.555e-38 Identity = 118/339 (34.81%), Postives = 171/339 (50.44%), Query Frame = 3
Query: 984 NQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGV---PLPSPEXXXXXXXXXXXXXXXXXETDSTND------------SNEQ------------------------GDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELV--NFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQN-------RCGYLLKP 1856
++P NHYFI SSHNTYL G Q+ S E Y QVL G RC+E+DIWDG E+ P+V HGF + +A+PL+ VI I + AF S +P+I+S E +C ++L++ I++E L G LPSP +LK KIL+K+KK + S N+ +NEQ G K II +S+ ++ + F +F + K ++ C SL E + + ++L N+R L R+YP R SSNF P FW AG Q+VA N+Q+ D+G QLNL +F++ + + GY+LKP
Sbjct: 383 SKPLNHYFIASSHNTYLLGKQIAETPSVEGYIQVLQQGCRCVEIDIWDG---ENGPVVCHGF-LTSAIPLKTVIRVIKKYAFITSPYPLIISLEINCNKDN---------QKLASLIMREVLAEQLYFVGTRTDKLPSPRELKHKILLKSKKTSEATRGLSVNEPFPSSFSSSYESANEQELRMKDDSTNSSSATNSSSMQRIKRIGLKKHADIINDVSN-ISGIHGIKFRNFSLPESKTIA-HCFSLNERKVEYMIKDKHLKLSLDKHNRRYLMRVYPHVLRYKSSNFNPIPFWKAGVQMVATNWQTNDIGQQLNLAMFQILDHQPDGSFKSGYVLKP 706
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Nematostella
Match: EDO43813 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXP6]) HSP 1 Score: 591.267 bits (1523), Expect = 0.000e+0 Identity = 323/746 (43.30%), Postives = 452/746 (60.59%), Query Frame = 3
Query: 264 KDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELPDTLEINM--LTKYFSQN-KDDREKLEDAXXXX---XXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTND---SNEQGDGDKGTEIIIPQI-------------------------SSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
+D ++ D SDE L+E+ I+I G + V+L + FVS + + D ++ N C LKK + KIC++ ++++ + KY S + K+DR++++DA+ S K+ I F+F+ F Y +L + ++ I+++I + + + + +FIN QRD RLNEILYP +K++E+I KYE + + G S QGL +Y+ SE+N + L QDM QP +HYFINSSHNTYLTGHQLTGKSS E+YRQ LL+G RCIELD WDGK + EP++THG T+CT + +DVI+AI E AFK SD+PVILSFENHCT KQ + S + +L +PL+ PLE G PLP P K+ + ++ KK E ++T+D SN TE P+I S +VNY +P F+SF+ + ++N S+E S E A L E PVE VN+NKRQLSRIYPKGTRV S N++PQ+FWNAGCQLVALNFQ+LDL M LNLG+FE RCGY+LKP MR SDR FDPF ES VDGI+A ++ + + SG +++K V Y+E+EM+GLPADTVRK+Y+ + G+ P + F+K++ P +A LRI E+ K +GQ LPVD + PGYR I L+N+ + L +P + ++ DYVP EDFA AL +PIAF S++++R QQL L ++
Sbjct: 3 QDQRLRDSLNIGSDED-PLTEKAITIVHGANMVDLMYVTFVSSSSTMARQWTDSLVKITHNLLEINECPTTFLKKQYTKICVQTTQDGKVSVKNVIKYISSSSKEDRKRIQDALQSVGLPHGKNDAISLEKFSFDIFWEFYNRLCNRQDIDKIFTEIGTKKK---PYMVTEQLVDFINNNQRDPRLNEILYPYCNEEKAREIIRKYEPNQDFVKKGHFSVQGLTRYLMSEDNAPIHPDRLSLHQDMTQPISHYFINSSHNTYLTGHQLTGKSSVEMYRQTLLAGCRCIELDCWDGKTEDQEPVITHGMTLCTEISFKDVIEAIAECAFKTSDYPVILSFENHCTPKQQLKMASYCVNIFGDMLLSKPLDDYPLEEGRPLP-PLKVLMRKIVIKNKKPIDSEPNATSDQAFSNNSAPPTPATEDKPPEINGEVDPDASQRDSTVEEEAAPDMALSELVNYFQPVHFKSFDVSAKRNRSYEMSSFVETVATTQLKEKPVEFVNYNKRQLSRIYPKGTRVGSENYMPQIFWNAGCQLVALNFQTLDLAMMLNLGIFEYNGRCGYILKPSFMRRSDRTFDPFAESTVDGIIAGAVHVKVISGQLLTEKKVGTYVEVEMYGLPADTVRKKYRTKVVPNNGINPVYDDDPFEFKKVVLPSLAVLRIAVYEESGKLIGQRILPVDGLNPGYRHIKLRNDCNLPLTLPVLFVHIITKDYVPSGFEDFANALCDPIAFQSQEDKRQQQLAALLDE 743
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Nematostella
Match: EDO38229 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDH9]) HSP 1 Score: 277.33 bits (708), Expect = 1.749e-80 Identity = 187/549 (34.06%), Postives = 279/549 (50.82%), Query Frame = 3
Query: 759 EFANFINQFQRDKR------LNEILYPLITNDKSKE----LISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTN-DSNEQGDGDKGTEI--------------------IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFI------SDKG--VYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIH-PKMAKLRILASTENT---KFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIY 2276
E +N+F K L + LY + SK+ +IS YE ++ + GL Y+ S+E +I D ++ ++ QDM QP +HYFI SSHNTYL QL G SS + Y G RC+ELD WDG +PIV HG T+ + + +D+I A+ E AFK SD+PVILS ENHC + + ++ L +K+ K+P+++ LPSPE LKGK+LIK KK +E + D D D+ +I + ++S VNY+ F++FE +KE ++ S E V +N RQLSR YP G+RVDSSN+ PQ+ WN GCQ+VALN+Q+ M LN G F R GY+LKP MR+ D F+P G+ L + + SG + S KG + Y+E++++G+ +D+ +K + G P + N +K+++ P++A LR E+ F+GQ LP+ +R GYR I L + + L S+ ++
Sbjct: 125 EIVEIMNKFTSGKAHMNVEDLQKFLYKQGVKEVSKQYCDIIISTYEPTEEGQRDKQLGIDGLTNYLLSKEGDIFDTAHDKVYQDMRQPLSHYFIASSHNTYLMEDQLKGPSSVDAYINAFNKGCRCVELDCWDGDN--GDPIVYHGHTLTSKIKFKDIIQAVAEHAFKVSDYPVILSLENHCNIENQRKIAEHLENILGDKLFKDPVDT----ALTSLPSPESLKGKVLIKGKKLKPEQEMAAVGEDDGTVSDEDEAADIEQEDLAAAREGEESKEKKVKLAKELSRCVNYISSVGFKNFEHSKENAKFYQMSSFVESKVATLAQNKAAGFVEYNTRQLSRTYPAGSRVDSSNYNPQVAWNVGCQIVALNYQTDGEYMHLNQGRFRENKRAGYVLKPAYMRNPDIKFNPNNPKSCPGVKKKLLIIQVISGQQLPKPQGSSSKGEVIDPYVEVKIYGVNSDSA--DFKTKVIKDNGFNPVW---NEEIKKLLYVPELAMLRFCVYDEDVGKDDFIGQFSLPITSLRTGYRHIHLLDIKGEPLPHASLFVH 662
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Nematostella
Match: EDO46546 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPQ0]) HSP 1 Score: 274.633 bits (701), Expect = 2.944e-77 Identity = 182/503 (36.18%), Postives = 268/503 (53.28%), Query Frame = 3
Query: 846 ELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETD------STNDSNEQGDGD------KGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSG-HFISDKGVYC-------YIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTEN---TKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEI 2285
E+IS+ E + + G +Y+ + +I D + QDM QP +HYFI SSHNTYL +QL+G+SS E Y L G RC+ELD WDG DEPI+ HG T+ + + ++ ++AI+E AF S +PVILS ENHC KQ + ++E L +K+ E P E LPSPE LKG+ILIKNKK A + D S D + +G + T + ++S +V+ + +F+ F+ + + +E CSL E A + N P E VN+NKR LSRIYP G RV SSN+ PQ WN GCQLVALN+Q+ + M +N G F CGY+LKP MR F+P + + GI +++ + SG F KG Y+ IE+FG+P+D ++R R+ G P F ++ F I P++A R + ++ F+GQ +P++ ++ G R + L L ++ ++ I
Sbjct: 262 EIISRCEPTEEGRKKKCLGIDGFTQYLMESDCDIFDPDQQNICQDMTQPLSHYFIASSHNTYLLDNQLSGRSSIEGYIAALQQGCRCVELDCWDGP--YDEPIIYHGHTLTSKIMFKETVEAIEEHAFDVSPYPVILSIENHCCVKQQQIMAKYLKEILKDKLYTEA----PGEDETFLPSPEALKGRILIKNKKLPASIDNDEEAGEVSEEDEYVEENGHDANKNPRKTTKLARELSDLVSLCKSVTFQDFQYSADNQKFWELCSLNEALARRLGNAYPEEFVNYNKRCLSRIYPSGKRVGSSNYNPQDMWNCGCQLVALNYQTPGIMMHVNSGKFLENGECGYVLKPSVMRDEIAYFNPNTKDAIPGISPQIVQIKVISGQQFPKPKGSTAKGDVIDPYVTIEVFGIPSDIAQER--TRTVPHNGFNPVFDET-FEFH-INLPEIALFRFVVQDDDFIGDGFIGQYTIPLNCMQQGDRHLRLLAFDGDPLDSATLFVHVTI 754
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Nematostella
Match: EDO31556 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXK4]) HSP 1 Score: 261.922 bits (668), Expect = 3.114e-76 Identity = 185/534 (34.64%), Postives = 276/534 (51.69%), Query Frame = 3
Query: 789 RDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQ----GLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGV-PLPSPE---XXXXXXXXXXXXXXXXXETDSTNDSNE---------QGDGDKGT-EIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESG-VDGIVANSLKLVL----------HSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIH-PKMAKLRILASTEN---TKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDD 2291
+D+R + +T+ KELI K+E +SQ G Q G + ++ S+ ++ + ++ ++ QDMNQP +HYFI SSHNTYL GHQL G SS + YR VL G RC+ELD WDG ++EPIV HG T+ + V +D++ AI ESAFK S +PVI+S ENHC+ + I IL L + P++ + LPSP+ K KE D DS + Q +GT E + ++S ++NY+ F+SF ++ E + S E + L+++ E V FNK Q+SRIYP RVDSSN+ P W GCQ+VALN Q+ MQL+ G F GY+LKP +R + FDP + G GI L+L + S + D+ Y+++E+ G+PAD + + T T L P + N FQ I+H P++A ++ + E+ F+GQ +P + + GYR I L + S L ++ ++ +I D
Sbjct: 36 KDQRFD------VTHKTCKELIKKFE----VSQRGKAQLQLGIDGFLSFINSQHASLFNPAHEKIYQDMNQPLSHYFIASSHNTYLLGHQLKGSSSVQAYRDVLERGCRCVELDCWDGD--DEEPIVYHGHTLTSKVLFKDIVQAIKESAFKVSPYPVIVSLENHCSVQ-----FQIKMAEHLKGILGSLLYTTPVDFTLDALPSPKFFLNKILVRGKTGKSIVDDKEMDKEIDSFDRKVQIERSVQETDFRGTKEHVADELSQLINYISNEKFKSFIESMEMGHFSQTHSFVESEMLYFLDKSASEFVEFNKHQISRIYPGNLRVDSSNYDPIKPWTVGCQIVALNHQTNSEEMQLHYGKFRQNGNTGYVLKPKFLRDPELTFDPDPQDGKFPGIQPKRLQLTVISAFQLLEIEESWSLVRDEPD-PYVQVEVHGVPAD--KATFTTHGLTDTTLNPVW---NEEFQTIVHVPELAMVQFVVYDEDYGFDDFLGQVVIPFESLMEGYRQIQLLDMSGLPLPGTTLFVHVKISD 546
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Nematostella
Match: EDO42237 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S279]) HSP 1 Score: 264.233 bits (674), Expect = 4.555e-73 Identity = 117/298 (39.26%), Postives = 189/298 (63.42%), Query Frame = 3
Query: 1506 IIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTED 2399
I P +SS+VNY++P F+ F++++++N+ ++ S E + P++ V +NKRQ+SRIYP+G+RVDSSN++PQ+FWN GCQ+V+LNFQ+ DL LN G FE R GY+LKP MR DR FDPF ES +DG V + + + SG ++SDK + +E++M+GLP DT+RK+YK +S+T GL + ++ F++++ P+MA LR +N + + Q +P+D I+ GYR + L+ E+ + + ++ ++ + YVP L+ PI F ++RA Q+ + D
Sbjct: 562 INPLLSSLVNYIQPVPFQGFDESEKRNLFYQMSSFNESTGFGLVRATPLDFVKYNKRQMSRIYPRGSRVDSSNYMPQVFWNVGCQMVSLNFQTPDLPFHLNQGKFEFNGRSGYILKPDLMRRDDRMFDPFTESPIDGYVGAYVAIKVISGQYLSDKKITTVVEVDMYGLPTDTIRKKYKTKSSTSNGLNTLYDGEHMVFRRVVLPEMALLRFAVIDDNDRIIAQRVVPLDSIQAGYRHVPLRTENDTPIPLATLFVHITLRCYVPEGYGAIVDELAEPIKFQCAADKRAAQMVVFGID 859 HSP 2 Score: 249.98 bits (637), Expect = 2.345e-68 Identity = 163/457 (35.67%), Postives = 244/457 (53.39%), Query Frame = 3
Query: 51 YKGMDISSNDILTEKCVKWEEA----EELTNCSIVNVT-VDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICL--ELPDTLEINMLTKYFSQNKDDREKLE--DAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQI-NSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
Y + + S +L+ +W+EA +E N +N T VD+ FF +W++ + PKD K+ + + +E ER ++ T+ V+ ++ + + T + + + + + N L L K + K L + + + + K K + L+ A+ SK+ I DFTFE F +Y K+ + LEI+ ++ ++ I +T+ NF+N+ QRD RLNEIL+P N+K+ LI KYE + + Q I GL +Y+ SE+N ++ L+ DMNQP +HYFINSSHNTYL G Q G+SS E+YRQ L++G RCIELD WDG+ DEPI+THG MCT + +DVI+AI +SAF+ SDFPVILSFENHC+ Q + + L + ++ +PLES PLE G PLPSP
Sbjct: 11 YVPLKLQSGTLLS----RWDEASFLNKEPNNKLRINTTYVDKCGFFLAWRSQDDKEGSSIEISNISEV--RLGVLPKDQKVRESLQRTGADPLE--ERGFTVVYNTNLVDCNYLHLFASTSRVAKEWVEGLRPLLHNVRLDNCSVLTQLHKQYVKAGLHKDALGKISVKSICKALYAGKFESLVLDALQALGLPCSKNDKITPEDFTFEHFYQLYLKVCPR--LEIM--ELCVEWGGGRAPILTATQLVNFMNKEQRDPRLNEILFPFCDNEKALSLIWKYEKQMDMLQRDRICVNGLTRYLLSEDNLLIMPGRLNLSDDMNQPLSHYFINSSHNTYLVGRQFGGRSSVEMYRQCLIAGCRCIELDCWDGQN--DEPIITHGNAMCTKILFKDVIEAIRDSAFQTSDFPVILSFENHCSKHQQYKMACYCMDILGDMLVTKPLESCPLEEGRPLPSP 453
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Medaka
Match: ENSORLT00000042446.1 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 [Source:NCBI gene;Acc:101175365]) HSP 1 Score: 547.74 bits (1410), Expect = 2.216e-175 Identity = 325/825 (39.39%), Postives = 481/825 (58.30%), Query Frame = 3
Query: 99 VKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL--PDTLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP-EXXXXXXXXXXXXXXXXXETDST--------------NDSNEQGD---------------GDKGTE----IIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIR-STTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILT-EDFMEANEREEESDGEPHEKS 2456
+KW+E +L+ + V + VD + F+ W TE++++++++D+R G+ K K+ K+ +L + + V L R +++ T +D VN++ NF++ ++ + + S S N L K + ++ L+L + + + + FS ++ E D+ + +I+ DFT E + + L + L I+ L N ++ + FIN QRD RLNEILYP ++ L+ +++ D + GLIS Q Y+ S+EN ++ + ++DM+ P +HYFINSSHNTYLT QL G SS E+YRQVLL+G RC+ELD+W G+ E+EP++THGFTM + + ++VI+AI E AFK S FPVILSFENH S KQ + + + +L +PL+ PLE GV LPSP E + ++ K TD+ + SN GD G KG+ + ++S++VNYV+PT F SFE +K+ + S E A++ L ++PVE V +NK QLSRIYPKGTRVDSSNF+PQLFWNAGCQLVALN+Q++DL MQLNL +FE R GY LKP MR D+ FDPF E+ VDGIVAN+L + + SG F++++ V Y+E+EMFGLPADT RK K + S + P + + I F+K+I P +A LRI A E KF+G +PV IRPGYR+I L+NE +QSLM+P++ +Y E+ DYVP D ALSNPI +++ E+R++QL LT ED E + E+E+D +++
Sbjct: 29 IKWDE--DLSTVTPVTLHVDPNGFYLYWTDQNKDTELLDLTVVKDVRTGRSTKTTKEAKLRELLD-VGNLVGRLENRMVTVVTASDLVNVNQLNFIASQEDEAKVWCEELFSLSSNLLSHNLNRDQSLLKAYVRLTLQLNTDGRIPVKNIVRLFSSDRKRVETALDSCRLPYGRGDSIRVEDFTLEVYKSFLDSLCPRPELGNIFK---LQGGNG-GMLSIDQMTEFINNKQRDPRLNEILYPPFRPAQTLSLMERHQLDQAELKRGLISLQAFSMYLSSDENGVIPPEKLDQSEDMSFPMSHYFINSSHNTYLTAGQLAGSSSVEMYRQVLLAGCRCVELDVWKGRTAEEEPVITHGFTMTSEISFKEVIEAIAECAFKTSLFPVILSFENHVDSLKQQAKMAEYCRSIFGDALLIDPLDKYPLEAGVALPSPLELMGKILIKNKKSHKPCSSTDTKRPIDQPANQIYEPLSPSNNTGDIEAESEEEDDDDDDDGKKGSAEREAVATEEMSTLVNYVQPTKFNSFEASKKAARCYHMSSFVETKALEHLTKSPVEFVEYNKSQLSRIYPKGTRVDSSNFMPQLFWNAGCQLVALNYQTIDLSMQLNLFMFEYNGRSGYRLKPEFMRRPDKHFDPFTENTVDGIVANTLSVKVISGQFLTERRVGVYVEVEMFGLPADTRRKALKTKTSQNNNAINPVWDEEPIVFKKVILPTLASLRIAAFEEGGKFIGHHIIPVSAIRPGYRYIGLRNERNQSLMLPAVFVYIEVKDYVPDTFADVIEALSNPIRYVNLLEQRSKQLAALTLEDGEEEAQTEDEADSTTEKRA 846
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Medaka
Match: PLCB1 (phospholipase C beta 1 [Source:NCBI gene;Acc:101172698]) HSP 1 Score: 328.946 bits (842), Expect = 2.558e-94 Identity = 157/305 (51.48%), Postives = 208/305 (68.20%), Query Frame = 3
Query: 1482 DGDKGTEIIIPQ-ISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILT 2393
+G G+E + +S++V Y++P F SFE +K+ N S++ S E A++ L + PVE V +NK QLSRIYPKGTRVDSSN+ PQLFWNAGCQLVALNFQ++DL MQLNLG+FE +CGY LKP MR D+ FDPF ES VDGIVAN+L + + SG F+SDK V Y+EI+MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G P IRPGY +I L+NE +Q L +PS+ +Y E+ DYVP D ALSNPI +++ E+RA QL LT
Sbjct: 550 EGTAGSEAFATEEMSNLVLYIQPVKFHSFETSKKINRSYQMSSFVETKALEQLTKCPVEFVEYNKLQLSRIYPKGTRVDSSNYNPQLFWNAGCQLVALNFQTVDLSMQLNLGMFEYNGKCGYRLKPEFMRRPDKHFDPFTESTVDGIVANTLSVKILSGQFLSDKKVGTYVEIDMFGLPVDTKRKAFKTKTSQGNAINPVWEEEAIVFKKVVLPTLASLRIAVFEEGGKFIGHRIFPASAIRPGYHYINLRNEKNQLLTLPSLFVYVEVKDYVPDTFADVIEALSNPIRYVNLMEQRANQLAALT 854 HSP 2 Score: 276.559 bits (706), Expect = 1.933e-76 Identity = 169/443 (38.15%), Postives = 261/443 (58.92%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPK---ILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL-PD-TLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
K +KWE+ + T + V +TVD +F W T++++++ I+D R GK+ K PKD K +LD+ T V +L R +++ +GTD VN+++ NF++ KE+ ++ L + + SR C L+K++ ++ L+L P+ + + + + FS ++ E ++ ++++I + DFT E + + + + L+ I+S+I S + + FIN QRD RLNEILYP + ++ + L+ KYE + L+Q G +S +G +Y+ EEN+I+ + ++DM P +HYFINSSHNTYLT QL G SS E+Y+QVLLSG RCIELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP+ILSFENH S KQ + + + +L EPLE LE GVPLPSP
Sbjct: 27 KFMKWED--DSTAVTPVTLTVDPKGYFLYWTDQNKDTDLLDVAFIKDARNGKFCKMPKDAKLREVLDISTL----VGKLENRMLTVVSGTDMVNITYLNFMAFQEEIAKEWAEELFKLASNLLVQNSSRETC----LEKVYTRLKLQLNPEGRIPVKNIFRIFSADRKRVETALESCNLPSGRNESIPQEDFTPEVYSMFLSNICPRPELDHIFSEIGA---KSRPYLTVEQMTEFINSKQRDPRLNEILYPPLKAEQVQVLVDKYEPNASLAQKGQMSVEGFSRYLNGEENSIIPPEKLDQSEDMTFPLSHYFINSSHNTYLTAGQLAGNSSVEMYKQVLLSGCRCIELDCWKGRTTEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPIILSFENHVDSPKQQAKMAEYCRSIFGDALLMEPLEKYQLEAGVPLPSP 456
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Medaka
Match: PLCB1 (phospholipase C beta 1 [Source:NCBI gene;Acc:101172698]) HSP 1 Score: 328.946 bits (842), Expect = 3.494e-94 Identity = 157/305 (51.48%), Postives = 208/305 (68.20%), Query Frame = 3
Query: 1482 DGDKGTEIIIPQ-ISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILT 2393
+G G+E + +S++V Y++P F SFE +K+ N S++ S E A++ L + PVE V +NK QLSRIYPKGTRVDSSN+ PQLFWNAGCQLVALNFQ++DL MQLNLG+FE +CGY LKP MR D+ FDPF ES VDGIVAN+L + + SG F+SDK V Y+EI+MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G P IRPGY +I L+NE +Q L +PS+ +Y E+ DYVP D ALSNPI +++ E+RA QL LT
Sbjct: 556 EGTAGSEAFATEEMSNLVLYIQPVKFHSFETSKKINRSYQMSSFVETKALEQLTKCPVEFVEYNKLQLSRIYPKGTRVDSSNYNPQLFWNAGCQLVALNFQTVDLSMQLNLGMFEYNGKCGYRLKPEFMRRPDKHFDPFTESTVDGIVANTLSVKILSGQFLSDKKVGTYVEIDMFGLPVDTKRKAFKTKTSQGNAINPVWEEEAIVFKKVVLPTLASLRIAVFEEGGKFIGHRIFPASAIRPGYHYINLRNEKNQLLTLPSLFVYVEVKDYVPDTFADVIEALSNPIRYVNLMEQRANQLAALT 860 HSP 2 Score: 277.715 bits (709), Expect = 1.086e-76 Identity = 169/443 (38.15%), Postives = 261/443 (58.92%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPK---ILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL-PD-TLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
K +KWE+ + T + V +TVD +F W T++++++ I+D R GK+ K PKD K +LD+ T V +L R +++ +GTD VN+++ NF++ KE+ ++ L + + SR C L+K++ ++ L+L P+ + + + + FS ++ E ++ ++++I + DFT E + + + + L+ I+S+I S + + FIN QRD RLNEILYP + ++ + L+ KYE + L+Q G +S +G +Y+ EEN+I+ + ++DM P +HYFINSSHNTYLT QL G SS E+Y+QVLLSG RCIELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP+ILSFENH S KQ + + + +L EPLE LE GVPLPSP
Sbjct: 27 KFMKWED--DSTAVTPVTLTVDPKGYFLYWTDQNKDTDLLDVAFIKDARNGKFCKMPKDAKLREVLDISTL----VGKLENRMLTVVSGTDMVNITYLNFMAFQEEIAKEWAEELFKLASNLLVQNSSRETC----LEKVYTRLKLQLNPEGRIPVKNIFRIFSADRKRVETALESCNLPSGRNESIPQEDFTPEVYSMFLSNICPRPELDHIFSEIGA---KSRPYLTVEQMTEFINSKQRDPRLNEILYPPLKAEQVQVLVDKYEPNASLAQKGQMSVEGFSRYLNGEENSIIPPEKLDQSEDMTFPLSHYFINSSHNTYLTAGQLAGNSSVEMYKQVLLSGCRCIELDCWKGRTTEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPIILSFENHVDSPKQQAKMAEYCRSIFGDALLMEPLEKYQLEAGVPLPSP 456
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Medaka
Match: PLCB1 (phospholipase C beta 1 [Source:NCBI gene;Acc:101172698]) HSP 1 Score: 328.176 bits (840), Expect = 3.992e-94 Identity = 157/305 (51.48%), Postives = 208/305 (68.20%), Query Frame = 3
Query: 1482 DGDKGTEIIIPQ-ISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILT 2393
+G G+E + +S++V Y++P F SFE +K+ N S++ S E A++ L + PVE V +NK QLSRIYPKGTRVDSSN+ PQLFWNAGCQLVALNFQ++DL MQLNLG+FE +CGY LKP MR D+ FDPF ES VDGIVAN+L + + SG F+SDK V Y+EI+MFGLP DT RK +K +++ + P + + I F+K++ P +A LRI E KF+G P IRPGY +I L+NE +Q L +PS+ +Y E+ DYVP D ALSNPI +++ E+RA QL LT
Sbjct: 556 EGTAGSEAFATEEMSNLVLYIQPVKFHSFETSKKINRSYQMSSFVETKALEQLTKCPVEFVEYNKLQLSRIYPKGTRVDSSNYNPQLFWNAGCQLVALNFQTVDLSMQLNLGMFEYNGKCGYRLKPEFMRRPDKHFDPFTESTVDGIVANTLSVKILSGQFLSDKKVGTYVEIDMFGLPVDTKRKAFKTKTSQGNAINPVWEEEAIVFKKVVLPTLASLRIAVFEEGGKFIGHRIFPASAIRPGYHYINLRNEKNQLLTLPSLFVYVEVKDYVPDTFADVIEALSNPIRYVNLMEQRANQLAALT 860 HSP 2 Score: 277.33 bits (708), Expect = 9.957e-77 Identity = 167/440 (37.95%), Postives = 261/440 (59.32%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVS----KTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL-PD-TLEINMLTKYFSQNKDDREKLEDAXXXXXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
K +KWE+ + T + V +TVD +F W T++++++ I+D R GK+ K PKD K+ ++ +S V +L R +++ +GTD VN+++ NF++ KE+ ++ L + + SR C L+K++ ++ L+L P+ + + + + FS ++ E ++ ++++I + DFT E + + + + L+ I+S+I S + + FIN QRD RLNEILYP + ++ + L+ KYE + L+Q G +S +G +Y+ EEN+I+ + ++DM P +HYFINSSHNTYLT QL G SS E+Y+QVLLSG RCIELD W G+ E+EP++THGFTM T + ++VI+AI E AFK S FP+ILSFENH S KQ + + + +L EPLE LE GVPLPSP
Sbjct: 27 KFMKWED--DSTAVTPVTLTVDPKGYFLYWTDQNKDTDLLDVAFIKDARNGKFCKMPKDAKLREVLD-ISTLVGKLENRMLTVVSGTDMVNITYLNFMAFQEEIAKEWAEELFKLASNLLVQNSSRETC----LEKVYTRLKLQLNPEGRIPVKNIFRIFSADRKRVETALESCNLPSGRNESIPQEDFTPEVYSMFLSNICPRPELDHIFSEIGA---KSRPYLTVEQMTEFINSKQRDPRLNEILYPPLKAEQVQVLVDKYEPNASLAQKGQMSVEGFSRYLNGEENSIIPPEKLDQSEDMTFPLSHYFINSSHNTYLTAGQLAGNSSVEMYKQVLLSGCRCIELDCWKGRTTEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPIILSFENHVDSPKQQAKMAEYCRSIFGDALLMEPLEKYQLEAGVPLPSP 456
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Medaka
Match: plcb3 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 [Source:NCBI gene;Acc:100049529]) HSP 1 Score: 317.005 bits (811), Expect = 4.536e-90 Identity = 157/308 (50.97%), Postives = 214/308 (69.48%), Query Frame = 3
Query: 1515 QISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQLEILTEDFMEANEREEESDG 2438
++S++VNYVEP F+SF+ A +K FE S E +++L +PVE V +NK QLSRIYPKGTRVDSSN++PQ+FWN GCQ+VALNFQ+LDL MQLN+G+FE R GYLLKP MR D+ FDPF ES VDGIVAN++K+ + SG F+++K V Y+E+++FGLPADT RK S+ L P + F K+I P +A LRI EN KF+G LP+ IRPGY +I LKNE SQ L++PS+LIY E+ DYVP + +++A AL++PI ++S+ +R +QL L +D +E + + D
Sbjct: 560 EMSNLVNYVEPVKFKSFDLAAKKRKYFEMSSFVETKGMEALKNSPVEFVEYNKNQLSRIYPKGTRVDSSNYMPQVFWNVGCQMVALNFQTLDLAMQLNMGVFEYNGRSGYLLKPEFMRRMDKHFDPFTESIVDGIVANTVKIKVVSGQFLTEKKVGVYVEVDIFGLPADTKRKYRTKTSSNSNSLDPVWDDEMFVFNKVILPSLASLRIAVCEENGKFIGHRILPISSIRPGYHYINLKNELSQPLLLPSLLIYIEVQDYVPNEHQEYADALTDPIKYISQLAKREKQLASLIDDNVEQQDSSQPKDS 867 HSP 2 Score: 256.914 bits (655), Expect = 7.766e-70 Identity = 171/443 (38.60%), Postives = 255/443 (57.56%), Query Frame = 3
Query: 93 KCVKWEEAEELTNCSIVNVTVDEHKFFFSWKTATG-ATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVELSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLEL--PDTLEINMLTKYFSQNKDDREKLEDAXXX---XXXXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQ--INSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTS-KQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPE 1394
K +KW+E + ++V + VD FF SW + EI++IS IRD R GKYA+ PK+ K+ + + D + + ++I G D VN+S+ NF + +++ + + S S N +L K + K+ L++ + + + K FS D+ ++E A+ +KS+ IK DFT+E F L L+ ++ I+ E+S K+ I+ +F +F+N+ QRD RLNE+LYP + ++ ++++ KYE++ IS + Y+ EEN I+ ++ DMNQP +HYFINSSHNTYLT QLTG SS E+YRQVLL+G RCIELD W + ++EP +THGFTM T +P +DVI+AI ESAFK S +PVILSFENH S K + + + +L +PLE PL PG PLPSP+
Sbjct: 27 KFIKWDEDPNGGSPTLVTLKVDPDGFFLSWTAGSNLEVEILDISTIRDTRTGKYARQPKEAKLQSMLSVGKDSFLG---KSLTIVYGNDLVNISYLNFQAMSEDEAKLWTEELFSLATNILSHNATRNSYLLKAYTKLKLQVNQEGKIPVKNIMKLFS----DKRRVETALEQCGLVRNKSEGIKPDDFTWEAFQKFLNNLCLRPEIQTIF------EESSKKKPFISLDQFMDFLNRRQRDSRLNEVLYPPLKREQVRQIMEKYETNSSQLDRDQISLRAFSLYLGGEENGIVPPERLDIIDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCW--QRQDEEPHITHGFTMTTEIPFKDVIEAIAESAFKTSPYPVILSFENHVESPKHQAKMAEYCRTIFGDALLIDPLEKYPLVPGQPLPSPQ 454
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Planmine SMEST
Match: SMESG000013960.1 (SMESG000013960.1) HSP 1 Score: 449.899 bits (1156), Expect = 1.036e-148 Identity = 274/408 (67.16%), Postives = 290/408 (71.08%), Query Frame = 3
Query: 924 MFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKIIHPKMAKLRILA 2147
MFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEKLKGKILIKNKKKAAPKETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAK+ LG LN EL N R + P R +F P F +G V N L + L+ G F SD+ ++E + G+ A++ VRKRYKIRSTTKTGLYPQFPKSNISFQK+I L +L+
Sbjct: 1 MFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEKLKGKILIKNKKKAAPKETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKD---------LG-----MQLNLGLFELQN---RCGYLLKPFEMRSSDRDFDP--FLESGVDGVVAN----SLKLVLHSGHF----------------ISDKGVYCYIEIEMFGLPADT-------------------------------VRKRYKIRSTTKTGLYPQFPKSNISFQKVISLMALILELLS 338
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Planmine SMEST
Match: SMESG000013960.1 (SMESG000013960.1) HSP 1 Score: 369.777 bits (948), Expect = 1.016e-117 Identity = 234/360 (65.00%), Postives = 248/360 (68.89%), Query Frame = 3
Query: 1035 TGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEXXXXXXXXXXXXXXXXXETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNISFQKII 2114
GHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEKLKGKILIKNKKKAAPKETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAK+ LG LN EL N R + P R +F P F +G V N L + L+ G F SD+ ++E + G+ A++ VRKRYKIRSTTKTGLYPQFPKSNISFQK+I
Sbjct: 76 AGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSPEKLKGKILIKNKKKAAPKETDSTNDSNEQGDGDKGTEIIIPQISSMVNYVEPTSFESFEKAKD---------LG-----MQLNLGLFELQN---RCGYLLKPFEMRSSDRDFDP--FLESGVDGVVAN----SLKLVLHSGHF----------------ISDKGVYCYIEIEMFGLPADT-------------------------------VRKRYKIRSTTKTGLYPQFPKSNISFQKVI 365
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Planmine SMEST
Match: SMESG000049234.1 (SMESG000049234.1) HSP 1 Score: 287.73 bits (735), Expect = 2.769e-80 Identity = 136/297 (45.79%), Postives = 194/297 (65.32%), Query Frame = 3
Query: 1494 GTEIIIPQISSMVNYVEPTSFESFEKAKEKNVSFECCSLGEIFAIQSLNENPVELVNFNKRQLSRIYPKGTRVDSSNFLPQLFWNAGCQLVALNFQSLDLGMQLNLGLFELQNRCGYLLKPFEMRSSDRDFDPFLESGVDGIVANSLKLVLHSGHFISDKGVYCYIEIEMFGLPADTVRKRYKIRSTTKTGLYPQFPKSNI-SFQKIIHPKMAKLRILASTENTKFVGQAWLPVDQIRPGYRFITLKNESSQSLMMPSMLIYFEIDDYVPCDMEDFALALSNPIAFLSEKERRAQQL 2381
T I P +S+MVNY +P F+ F+ A+E+ F S E A+ L +E VN+NKRQLSRIYP+G RVDS+NF+PQ+FWNAGCQ+VALNFQ+ DL MQLN G FE GYLLKP MR +R FDPF ES +DG++A +L++ + SG F+SDK V Y+E++M+GLP DT RK ++ R GL P + ++ F+K++ P +A LRI + K +GQ LP++ ++ GYR I+L+ E + L +P++ Y+P + D ALS+P A+LS++E+R +QL
Sbjct: 595 ATTNIHPYLSAMVNYTQPVKFQGFDIAEEEAYHFHMSSFSETVALGYLKNEAIEFVNYNKRQLSRIYPRGNRVDSANFMPQIFWNAGCQMVALNFQTPDLAMQLNQGKFEYNGNSGYLLKPEFMRRPERSFDPFSESPMDGVIAATLEIKIISGQFLSDKKVGTYVEVDMYGLPTDTFRKEFRTRVVPNNGLNPIYGDDSVFLFRKVVLPDLAVLRIGVYEDTGKLIGQRVLPLEGLQSGYRHISLRTEGNFPLSLPTIFCKITQTTYIPEGLVDLVDALSDPRAYLSKEEKRMKQL 891 HSP 2 Score: 273.092 bits (697), Expect = 1.962e-75 Identity = 161/427 (37.70%), Postives = 245/427 (57.38%), Query Frame = 3
Query: 126 TNCSIVNVTVDEHKFFFSWKTATGATXXXXXXXXXXXXXGKYAKFPKDPKILDLCTALSDEVVE-LSERCISICTGTDFVNLSWYNFVSKTKEFPTKIHDLIISQIKLPKSRNPCHLVHLKKIWAKICLELP--DTLEINMLTKYFSQNKDDREKLEDAXXXXX--XXXXXXXXXDFTFERFLVIYEKLFLQDFLEIIYSKIALDHENSVKQINSTEFANFINQFQRDKRLNEILYPLITNDKSKELISKYESDVLLSQNGLISSQGLIKYMFSEENNILDQSNYELTQDMNQPFNHYFINSSHNTYLTGHQLTGKSSAEIYRQVLLSGSRCIELDIWDGKGMEDEPIVTHGFTMCTAVPLRDVIDAIDESAFKFSDFPVILSFENHCTSKQLPTLVSIVQERLSNKILKEPLESNPLEPGVPLPSP 1391
+NC V VDE+ FF WK+ G +++++S + D+ ++ PK+ KI C L + + L +R ++IC+GTD VN+S+ + VS+ E T + + K N C ++ LKK W ++CL + + + ++K F+ K ++ + K+ I S FTFE+F +Y K+ + +E I+ +++ NS I + + ++N+ QRD RLNEIL+P D K ++ E + G +S + +Y+ S++N + + QDM+QP HY+INSSHNTYLTG Q GKSS EIYRQVLLSG RCIELD WDGKG + EPI+THG MCT + +DVI AI ++AF S+ P+ILSFENHC+ Q L ++ L + +L +P++ + L VPLPSP
Sbjct: 48 SNCL---VRVDEYGFFVHWKSEQGEGQVLDLSQVSDV---RFGSKPKEDKI---CAVLLERISNPLDDRIVTICSGTDLVNISYTHMVSQDNEIATFWINNLRPLTHNTKMNNVCPMIQLKKHWLRLCLGVKANGMIPVRSISKTFASGKTEKIIFQSLKELGLPFGKNDEIDPSSFTFEKFYELYHKICPRTDIEEIFKELS----NSKDFITTEKIIEYLNEVQRDPRLNEILFPFANKDTVKYILDNNEPSEEYRKEGKLSIECFYRYLMSDDNAPVFLDRLNICQDMDQPLAHYYINSSHNTYLTGRQFGGKSSVEIYRQVLLSGCRCIELDCWDGKGEDQEPIITHGKAMCTDILFKDVIYAIRDTAFVTSEMPIILSFENHCSKPQQYKLAKYCEDVLGDLLLTKPIDKHELSQAVPLPSP 461
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Planmine SMEST
Match: SMESG000013964.1 (SMESG000013964.1) HSP 1 Score: 259.61 bits (662), Expect = 3.896e-80 Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 3
Query: 2955 LSQIDSKSKEILSNINVNQANEIKAINVKYINLFYEELYASMESDISRFQKRIDKIYRKECEVLKSLQHYKFKCETKFLQRECSNKDEFNRLKREALQKYIQCSVSELQKLTDLKNIRAKDLENNIIIASXXXXXXXXXXXXLFC 3389
LSQIDSKSKEILSNINVNQANEIKAINVKYINLFYEELYASMESDISRFQKRIDKIYRKECEVLKSLQHYKFKCETKFLQRECSNKDEFNRLKREALQKYIQCSVSELQKLTDLKN+RAKDLENNIIIASEKFKNDKEKDFKLFC
Sbjct: 20 LSQIDSKSKEILSNINVNQANEIKAINVKYINLFYEELYASMESDISRFQKRIDKIYRKECEVLKSLQHYKFKCETKFLQRECSNKDEFNRLKREALQKYIQCSVSELQKLTDLKNMRAKDLENNIIIASEKFKNDKEKDFKLFC 164
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Planmine SMEST
Match: SMESG000013964.1 (SMESG000013964.1) HSP 1 Score: 259.61 bits (662), Expect = 6.211e-80 Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 3
Query: 2955 LSQIDSKSKEILSNINVNQANEIKAINVKYINLFYEELYASMESDISRFQKRIDKIYRKECEVLKSLQHYKFKCETKFLQRECSNKDEFNRLKREALQKYIQCSVSELQKLTDLKNIRAKDLENNIIIASXXXXXXXXXXXXLFC 3389
LSQIDSKSKEILSNINVNQANEIKAINVKYINLFYEELYASMESDISRFQKRIDKIYRKECEVLKSLQHYKFKCETKFLQRECSNKDEFNRLKREALQKYIQCSVSELQKLTDLKN+RAKDLENNIIIASEKFKNDKEKDFKLFC
Sbjct: 29 LSQIDSKSKEILSNINVNQANEIKAINVKYINLFYEELYASMESDISRFQKRIDKIYRKECEVLKSLQHYKFKCETKFLQRECSNKDEFNRLKREALQKYIQCSVSELQKLTDLKNMRAKDLENNIIIASEKFKNDKEKDFKLFC 173 The following BLAST results are available for this feature:
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014607 ID=SMED30014607|Name=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase|organism=Schmidtea mediterranea sexual|type=transcript|length=3441bpback to top protein sequence of SMED30014607-orf-1 >SMED30014607-orf-1 ID=SMED30014607-orf-1|Name=SMED30014607-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1119bp MDYCYYKGMDISSNDILTEKCVKWEEAEELTNCSIVNVTVDEHKFFFSWKback to top Aliases
The feature '1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
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