SMED30014551

Overview
NameSMED30014551
Smed IDSMED30014551
Length (bp)2370
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014551 (SMED30014551) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014551 (SMED30014551) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014551 (SMED30014551) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014551 (SMED30014551) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014551

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014551SMESG000019910.1 SMESG000080909.1 Contig45511newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014551SMESG000019910.1 SMESG000080909.1 Contig45511uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30014551SMESG000080911.1 SMESG000080909.1 dd_Smed_v4_8194_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30014551 vs. Planmine SMEST
Match: SMESG000080909.1 (SMESG000080909.1)

HSP 1 Score: 558.14 bits (1437), Expect = 0.000e+0
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 2 
Query: 1247 MILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIXXXXXXXXXXXXXXXDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVINDQ 2122
            MILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVINDQ
Sbjct:    1 MILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVINDQ 292
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BLAST of SMED30014551 vs. Planmine SMEST
Match: SMESG000080909.1 (SMESG000080909.1)

HSP 1 Score: 553.903 bits (1426), Expect = 0.000e+0
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 2 
Query: 1247 MILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIXXXXXXXXXXXXXXXDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVIN 2116
            MILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVIN
Sbjct:    1 MILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVIN 290
[Close]
BLAST of SMED30014551 vs. Planmine SMEST
Match: SMESG000080909.1 (SMESG000080909.1)

HSP 1 Score: 546.584 bits (1407), Expect = 0.000e+0
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 2 
Query: 1265 MKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIXXXXXXXXXXXXXXXDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVINDQ 2122
            MKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVINDQ
Sbjct:    1 MKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVINDQ 286
[Close]
BLAST of SMED30014551 vs. Planmine SMEST
Match: SMESG000080909.1 (SMESG000080909.1)

HSP 1 Score: 542.347 bits (1396), Expect = 0.000e+0
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 2 
Query: 1265 MKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIXXXXXXXXXXXXXXXDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVIN 2116
            MKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVIN
Sbjct:    1 MKKSSTPVSRPDTNLISIVRSVVTMTKSPQKDDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRMYKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCIDRKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFLNVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLSLNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSAVIN 284
[Close]
BLAST of SMED30014551 vs. Planmine SMEST
Match: SMESG000080911.1 (SMESG000080911.1)

HSP 1 Score: 486.878 bits (1252), Expect = 9.306e-169
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 2 
Query:  158 MSRLVIMEGWINQKTKTKEICEVYVVLTKSHLKVYKSQINYKQRPNDTLRVLELSENYNILPMSFDNEYTFSIFKKGEKASERLYLHATSKADYEFWLNQIEGVLNPKNRENDINSSNGENLVVNEKRVSAEPSEKSPIIKATYNIKNSQNSVIQPDKSEIKNLQEKCLKLEGEMQKNQLFIEKCIEENDKLRKELECSIIDMTKLKELHAKLTIENETLKKTLSQTEVKLQKSNDDVRRIKL 886
            MSRLVIMEGWINQKTKTKEICEVYVVLTKSHLKVYKSQINYKQRPNDTLRVLELSENYNILPMSFDNEYTFSIFKKGEKASERLYLHATSKADYEFWLNQIEGVLNPKNRENDINSSNGENLVVNEKRVSAEPSEKSPIIKATYNIKNSQNSVIQPDKSEIKNLQEKCLKLEGEMQKNQLFIEKCIEENDKLRKELECSIIDMTKLKELHAKL IENETLKKT SQTEVKLQKSNDDVRRI L
Sbjct:    1 MSRLVIMEGWINQKTKTKEICEVYVVLTKSHLKVYKSQINYKQRPNDTLRVLELSENYNILPMSFDNEYTFSIFKKGEKASERLYLHATSKADYEFWLNQIEGVLNPKNRENDINSSNGENLVVNEKRVSAEPSEKSPIIKATYNIKNSQNSVIQPDKSEIKNLQEKCLKLEGEMQKNQLFIEKCIEENDKLRKELECSIIDMTKLKELHAKLIIENETLKKTFSQTEVKLQKSNDDVRRINL 243
The following BLAST results are available for this feature:
BLAST of SMED30014551 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014551 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000080909.10.000e+0100.00SMESG000080909.1[more]
SMESG000080909.10.000e+0100.00SMESG000080909.1[more]
SMESG000080909.10.000e+0100.00SMESG000080909.1[more]
SMESG000080909.10.000e+0100.00SMESG000080909.1[more]
SMESG000080911.19.306e-16998.77SMESG000080911.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014551 ID=SMED30014551|Name=SMED30014551|organism=Schmidtea mediterranea sexual|type=transcript|length=2370bp
CGTCAAATGACATTGTCAAATAAAATCAATGGAGAATTGTCACTGCTGAT
TATCTGTAATAAAATTGTAGAGTTGATCAATCAGAGCTCATTACAATTTA
ATATCTACTTTAACTGTAATTTTAAATTTTTTCGCAACAATTTTTGTCGA
GCTAAACATGTCAAGATTAGTGATTATGGAAGGATGGATTAATCAAAAAA
CCAAGACAAAAGAAATCTGTGAAGTTTATGTTGTTTTGACGAAATCGCAC
CTGAAAGTCTATAAATCACAAATCAACTACAAACAACGACCAAATGATAC
TTTAAGAGTTTTAGAATTATCCGAAAATTACAATATTCTCCCAATGTCAT
TTGACAATGAATATACATTCAGTATTTTCAAAAAAGGTGAAAAAGCATCA
GAAAGGCTTTATCTCCATGCAACATCGAAAGCTGATTATGAATTCTGGTT
AAATCAGATTGAAGGAGTCCTTAATCCTAAAAACAGAGAAAATGATATAA
ATTCTTCGAATGGTGAAAATCTTGTTGTAAATGAAAAACGCGTGTCAGCA
GAACCATCTGAAAAATCTCCAATAATAAAGGCAACATATAACATTAAAAA
TTCACAAAACAGTGTAATCCAACCGGATAAGAGTGAAATCAAAAATTTGC
AAGAAAAGTGTTTGAAATTAGAAGGAGAAATGCAAAAAAATCAACTTTTT
ATTGAGAAATGTATAGAGGAAAACGATAAACTTCGAAAAGAATTAGAATG
TAGCATAATCGATATGACGAAACTAAAAGAGCTCCATGCTAAACTTACCA
TTGAAAATGAAACTTTAAAGAAAACCCTTTCTCAGACAGAAGTAAAACTT
CAAAAAAGCAATGACGACGTTCGACGAATCAAACTTGAAAAGCAGCACAT
AGTGGATAAGAAAATGGGAACTGATAAAGAGCTTGAAACCTTGAAAAAGT
CTTTATCATCAAAAATTGAAAAAGAAGAAAATCAAATGAGTCAAATATTG
ACTGATCTCAGCAGCACAATTTGTTCAACCAATGTCGACCTGTCATCCTA
TATATTTTGTGTTGATTTTCTAACGGAAAAATACAAATCATCCGTCACTA
TCGAGTCCCTTTTTAAACAGCAATTAGTCAATTTGATCCACAGGTGCCCA
ATTGATCTCAACGGCAACATAACAATTCTCGATTATTTGACTGTCCGAAT
TGATCTCTCACAAGATTGTGCCATTGATTTCATTTGCAAATTATTGATGA
TACTCAGCAATTTCATGAAAAAATCCTCAACCCCAGTTTCGAGACCCGAC
ACAAATCTCATATCAATAGTCAGAAGCGTCGTCACCATGACAAAATCACC
TCAGAAAGACGATTTGAAATTGAATTTTCATCAAACATCTATGGAAATTG
CATATTTGTTGATCAAAAAAACCAACACAAATCCTCAGTTCACGAATGGG
ATTTCAGACGATGTTACGAAAAAAGTTGATAGTCAATATGAGGAATTTCT
CAGAATGTACAAATTTGATACAACAAAGAAACTGATTTTAACGGAGCTGG
GTGAACAAATGAAGGAAAGGTTTCAAGCACAGTTAGAAAATAAAGAAAAT
TTGATGAAACCAAAGTTGGCGGAGCAGTCTGATTCTATGATTCGGAAATG
TATTGACAGAAAATGGAGTGAAATGCTAAAATGTTTGGAGGATAAAAATC
AAGAAATAAACGACTTGACAATAGTTGTCCGCGAACTTTCCGAAAGGGTT
TTAGTCTTGGAATCAAGCGATAACTCAAATTCTCAATCTTTATATGATGA
ATTTTTAAACGTTTATTCGATGAATTCAGAAATTCTTTGCTCAACTTGCC
AGTTTCGAGCCGTGGAACTTCTCGAAATGCCAATAATTGAGAAAATGAAT
AAAATCGTGTCAGATTTTAAATCTTATTGGAAATCTATATTAAATATTCA
TTTGTCGCTCAATTTAAAACAGAAGTCAATTTTCGGAATTCGGGATATTT
TTGAGAAAATGACTCAATCAACTGAGGAGTTCGAGTCGGTAATGAAGCAA
TTTTCAGAGATCACTAGCAGAAAAATAGAGAATCCACTGAATCAAAAGTC
AAGTGCGGTTATAAATGATCAATAAACACAACTCGCTGTTCAATGAAATT
CTGATCTATGTTCAACAGATCAGGATTATTGATTGATTTATTCCTCGGTT
GCCATCTCGAAATTGTATTTATTCAATTATTAATTTATATAATTTACTTA
TTTACAATAGTAATTTTGATCTTTATGTATTTTCTTGTTCCAATAATGCT
CTATTCTTTTCCTGTTCATTTGTGTGATACATTGGATATTCTTGATTACT
TTTTATTAATTATATAGAGG
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protein sequence of SMED30014551-orf-1

>SMED30014551-orf-1 ID=SMED30014551-orf-1|Name=SMED30014551-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=656bp
MSRLVIMEGWINQKTKTKEICEVYVVLTKSHLKVYKSQINYKQRPNDTLR
VLELSENYNILPMSFDNEYTFSIFKKGEKASERLYLHATSKADYEFWLNQ
IEGVLNPKNRENDINSSNGENLVVNEKRVSAEPSEKSPIIKATYNIKNSQ
NSVIQPDKSEIKNLQEKCLKLEGEMQKNQLFIEKCIEENDKLRKELECSI
IDMTKLKELHAKLTIENETLKKTLSQTEVKLQKSNDDVRRIKLEKQHIVD
KKMGTDKELETLKKSLSSKIEKEENQMSQILTDLSSTICSTNVDLSSYIF
CVDFLTEKYKSSVTIESLFKQQLVNLIHRCPIDLNGNITILDYLTVRIDL
SQDCAIDFICKLLMILSNFMKKSSTPVSRPDTNLISIVRSVVTMTKSPQK
DDLKLNFHQTSMEIAYLLIKKTNTNPQFTNGISDDVTKKVDSQYEEFLRM
YKFDTTKKLILTELGEQMKERFQAQLENKENLMKPKLAEQSDSMIRKCID
RKWSEMLKCLEDKNQEINDLTIVVRELSERVLVLESSDNSNSQSLYDEFL
NVYSMNSEILCSTCQFRAVELLEMPIIEKMNKIVSDFKSYWKSILNIHLS
LNLKQKSIFGIRDIFEKMTQSTEEFESVMKQFSEITSRKIENPLNQKSSA
VINDQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001849PH_domain
IPR011993PH-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 258..278
NoneNo IPR availableCOILSCoilCoilcoord: 4..31