Intraflagellar transport protein 43

Overview
NameIntraflagellar transport protein 43
Smed IDSMED30014550
Length (bp)230
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Intraflagellar transport protein 43 (SMED30014550) t-SNE clustered cells

Violin plots show distribution of expression levels for Intraflagellar transport protein 43 (SMED30014550) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30014550

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30014550h1SMcG0016281 SmedASXL_004841SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30014550h1SMcG0016281 dd_Smed_v4_5237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30014550h1SMcG0016281 dd_Smed_v4_5237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014550h1SMcG0016281 dd_Smed_v4_5237_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014550h1SMcG0016281 dd_Smed_v6_5237_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30014550h1SMcG0016281 dd_Smed_v4_5237_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Human
Match: IFT43 (intraflagellar transport 43 [Source:HGNC Symbol;Acc:HGNC:29669])

HSP 1 Score: 52.373 bits (124), Expect = 2.603e-9
Identity = 32/75 (42.67%), Postives = 43/75 (57.33%), Query Frame = 2 
Query:   17 YGTLDIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            YG  DIP IP  E   EE    Q+A  P + + RV TY++LD DL ++ A    + EIDLK+L+  L  + EV E
Sbjct:   96 YGG-DIPIIPDLEEVQEEDFVLQVAAPPSIQIKRVMTYRDLDNDLMKYSAIQTLDGEIDLKLLTKVLAPEHEVRE 169
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Human
Match: IFT43 (intraflagellar transport 43 [Source:HGNC Symbol;Acc:HGNC:29669])

HSP 1 Score: 52.373 bits (124), Expect = 2.603e-9
Identity = 32/75 (42.67%), Postives = 43/75 (57.33%), Query Frame = 2 
Query:   17 YGTLDIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            YG  DIP IP  E   EE    Q+A  P + + RV TY++LD DL ++ A    + EIDLK+L+  L  + EV E
Sbjct:   96 YGG-DIPIIPDLEEVQEEDFVLQVAAPPSIQIKRVMTYRDLDNDLMKYSAIQTLDGEIDLKLLTKVLAPEHEVRE 169
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Human
Match: IFT43 (intraflagellar transport 43 [Source:HGNC Symbol;Acc:HGNC:29669])

HSP 1 Score: 51.2174 bits (121), Expect = 6.435e-9
Identity = 30/71 (42.25%), Postives = 41/71 (57.75%), Query Frame = 2 
Query:   29 DIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP  E   EE    Q+A  P + + RV TY++LD DL ++ A    + EIDLK+L+  L  + EV E
Sbjct:  104 DIPIIPDLEEVQEEDFVLQVAAPPSIQIKRVMTYRDLDNDLMKYSAIQTLDGEIDLKLLTKVLAPEHEVRE 174
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Xenopus
Match: rgs7bp (regulator of G-protein signaling 7 binding protein [Source:Xenbase;Acc:XB-GENE-1001518])

HSP 1 Score: 59.6918 bits (143), Expect = 4.018e-12
Identity = 35/71 (49.30%), Postives = 42/71 (59.15%), Query Frame = 2 
Query:   29 DIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP  E   EE    Q+A  P V VNRV TYK+LD DL  H AF   + +IDLK+L+  L  + EV E
Sbjct:   91 DIPVIPDLEDVQEEDLALQVASPPSVQVNRVLTYKDLDNDLMRHAAFQTLDGDIDLKLLTKVLSPEPEVRE 161
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Mouse
Match: Ift43 (intraflagellar transport 43 [Source:MGI Symbol;Acc:MGI:1923661])

HSP 1 Score: 54.299 bits (129), Expect = 2.927e-10
Identity = 25/53 (47.17%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:   68 QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV E
Sbjct:  104 QVASPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVLAPEHEVRE 156
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Mouse
Match: Ift43 (intraflagellar transport 43 [Source:MGI Symbol;Acc:MGI:1923661])

HSP 1 Score: 53.9138 bits (128), Expect = 3.965e-10
Identity = 25/53 (47.17%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:   68 QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV E
Sbjct:  115 QVASPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVLAPEHEVRE 167
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Mouse
Match: Ift43 (intraflagellar transport 43 [Source:MGI Symbol;Acc:MGI:1923661])

HSP 1 Score: 51.2174 bits (121), Expect = 1.841e-9
Identity = 22/45 (48.89%), Postives = 31/45 (68.89%), Query Frame = 2 
Query:   68 QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCL 202
            Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L
Sbjct:   94 QVASPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVL 138
[Close]
BLAST of Intraflagellar transport protein 43 vs. UniProt/SwissProt
Match: sp|Q28CW2|IFT43_XENTR (Intraflagellar transport protein 43 homolog OS=Xenopus tropicalis OX=8364 GN=ift43 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.129e-11
Identity = 35/71 (49.30%), Postives = 42/71 (59.15%), Query Frame = 2 
Query:   29 DIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP  E   EE    Q+A  P V VNRV TYK+LD DL  H AF   + +IDLK+L+  L  + EV E
Sbjct:   91 DIPVIPDLEDVQEEDLALQVASPPSVQVNRVLTYKDLDNDLMRHAAFQTLDGDIDLKLLTKVLSPEPEVRE 161
[Close]
BLAST of Intraflagellar transport protein 43 vs. UniProt/SwissProt
Match: sp|Q3B8E9|IFT43_XENLA (Intraflagellar transport protein 43 homolog OS=Xenopus laevis OX=8355 GN=ift43 PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 8.042e-11
Identity = 33/71 (46.48%), Postives = 42/71 (59.15%), Query Frame = 2 
Query:   29 DIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP  E   EE    Q+A  P V VNRV TY++LD DL  H AF   + ++DLK+L+  L  + EV E
Sbjct:   91 DIPVIPDLEDVQEEDLALQVASPPSVQVNRVMTYRDLDNDLMRHAAFQSLDGDVDLKLLTKVLSPEPEVRE 161
[Close]
BLAST of Intraflagellar transport protein 43 vs. UniProt/SwissProt
Match: sp|B5X7E4|IF43B_SALSA (Intraflagellar transport protein 43 homolog B OS=Salmo salar OX=8030 GN=ift43b PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 9.158e-11
Identity = 33/74 (44.59%), Postives = 44/74 (59.46%), Query Frame = 2 
Query:   20 GTLDIPTIPEAS--NEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            G  DIP IP+     EE    Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV E
Sbjct:  100 GEGDIPVIPDLDEVQEEDLNMQVAAPPSIQVNRVMTYRDLDNDLMKYSAFRTLDGEIDLKLLTKVLAPEQEVRE 173
[Close]
BLAST of Intraflagellar transport protein 43 vs. UniProt/SwissProt
Match: sp|E2QSX5|IFT43_CANLF (Intraflagellar transport protein 43 homolog OS=Canis lupus familiaris OX=9615 GN=IFT43 PE=3 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 1.147e-10
Identity = 33/71 (46.48%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:   29 DIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP  E   EE    Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV E
Sbjct:  100 DIPVIPDLEDVQEEDFALQVAAPPSIQVNRVMTYRDLDNDLMKYAAFQTLDGEIDLKLLTKVLAPEHEVRE 170
[Close]
BLAST of Intraflagellar transport protein 43 vs. UniProt/SwissProt
Match: sp|Q2TBN9|IFT43_BOVIN (Intraflagellar transport protein 43 homolog OS=Bos taurus OX=9913 GN=IFT43 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 2.347e-10
Identity = 33/71 (46.48%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:   29 DIPTIP--EASNEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP  E   EE    Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV E
Sbjct:   98 DIPVIPDLEEVQEEDFVLQVAAPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVLAPEHEVRE 168
[Close]
BLAST of Intraflagellar transport protein 43 vs. TrEMBL
Match: R7U7U5 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_18761 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 9.486e-14
Identity = 37/79 (46.84%), Postives = 53/79 (67.09%), Query Frame = 2 
Query:    5 RAVTYGTLDIPTIPEASNEE-----PQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            +A + G  DIPTIP+  ++       Q+A AP V+VNRV+TY+ELD DL  H AF   ++EIDLKILS  L  +++V++
Sbjct:  119 KAESDGGQDIPTIPDLEDQGEDDLTAQVAMAPNVVVNRVATYRELDNDLLRHAAFLTLDNEIDLKILSKGLSAEADVQD 197
[Close]
BLAST of Intraflagellar transport protein 43 vs. TrEMBL
Match: A0A1I8HV32 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025147g3 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 6.761e-13
Identity = 28/57 (49.12%), Postives = 43/57 (75.44%), Query Frame = 2 
Query:   56 NEEPQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            NE P++A+AP + VNRV+TY+ELD DL+ H +F   ++EIDLK+L+ CL    +++E
Sbjct:   98 NEPPEMAKAPSIAVNRVATYRELDFDLNRHASFATLDNEIDLKLLTRCLAADQDLQE 154
[Close]
BLAST of Intraflagellar transport protein 43 vs. TrEMBL
Match: A0A267DWM3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025147g2 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 7.172e-13
Identity = 28/57 (49.12%), Postives = 43/57 (75.44%), Query Frame = 2 
Query:   56 NEEPQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            NE P++A+AP + VNRV+TY+ELD DL+ H +F   ++EIDLK+L+ CL    +++E
Sbjct:  101 NEPPEMAKAPSIAVNRVATYRELDFDLNRHASFATLDNEIDLKLLTRCLAADQDLQE 157
[Close]
BLAST of Intraflagellar transport protein 43 vs. TrEMBL
Match: A0A0P4W2M4 (Uncharacterized protein OS=Scylla olivacea OX=85551 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.929e-12
Identity = 33/53 (62.26%), Postives = 41/53 (77.36%), Query Frame = 2 
Query:   68 QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            QIAQAP V VNRV+TYKELD DL  H AF+   D+IDL++L++CL  +SEV E
Sbjct:  116 QIAQAPSVAVNRVATYKELDSDLLRHAAFSTL-DDIDLRLLTSCLAPESEVRE 167
[Close]
BLAST of Intraflagellar transport protein 43 vs. TrEMBL
Match: A0A3R7PLZ3 (Putative intraflagellar transport protein 43-like A-like isoform X2 OS=Penaeus vannamei OX=6689 GN=C7M84_010907 PE=4 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.977e-12
Identity = 39/71 (54.93%), Postives = 48/71 (67.61%), Query Frame = 2 
Query:   29 DIPTIPEASN--EE---PQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP+     EE    QIAQAP V VNRV+TYKELD DL  H AF+   D+IDL+ L+NCL  +S++ E
Sbjct:   95 DIPVIPDLDEVVEEDFTQQIAQAPSVAVNRVATYKELDSDLLRHAAFSTL-DDIDLRQLTNCLSNESDIRE 164
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Cavefish
Match: IFT43 (intraflagellar transport 43 [Source:NCBI gene;Acc:103033244])

HSP 1 Score: 58.5362 bits (140), Expect = 4.263e-12
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:   29 DIPTIPEAS--NEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEEV 229
            DIP IP+     EE    Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV EV
Sbjct:   75 DIPVIPDLDEVQEEDLTMQVAAPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVLAPEQEVREV 146
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Cavefish
Match: IFT43 (intraflagellar transport 43 [Source:NCBI gene;Acc:103033244])

HSP 1 Score: 58.151 bits (139), Expect = 6.054e-12
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 2 
Query:   29 DIPTIPEAS--NEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEEV 229
            DIP IP+     EE    Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV EV
Sbjct:   80 DIPVIPDLDEVQEEDLTMQVAAPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVLAPEQEVREV 151
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Cavefish
Match: IFT43 (intraflagellar transport 43 [Source:NCBI gene;Acc:103033244])

HSP 1 Score: 56.6102 bits (135), Expect = 3.002e-11
Identity = 32/71 (45.07%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:   29 DIPTIPEAS--NEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP+     EE    Q+A  P + VNRV TY++LD DL ++ AF   + EIDLK+L+  L  + EV E
Sbjct:   80 DIPVIPDLDEVQEEDLTMQVAAPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEIDLKLLTKVLAPEQEVRE 150
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008891.1 (pep scaffold:Pmarinus_7.0:GL476825:10523:32821:-1 gene:ENSPMAG00000008049.1 transcript:ENSPMAT00000008891.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 2.781e-12
Identity = 29/71 (40.85%), Postives = 42/71 (59.15%), Query Frame = 2 
Query:   29 DIPTIPEASNEE-----PQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP+  + +      Q+A  P + VNRV T+K+LD DL  H AF   + +IDLK+L+  L  + E+ E
Sbjct:   63 DIPVIPDLDDMQEDDLTAQVAAPPSMQVNRVMTFKDLDSDLMTHAAFQTLDGDIDLKLLTRVLAPEPEIRE 133
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Nematostella
Match: EDO42250 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2A2])

HSP 1 Score: 55.4546 bits (132), Expect = 1.538e-11
Identity = 32/65 (49.23%), Postives = 40/65 (61.54%), Query Frame = 2 
Query:   32 IPTIPEASNEE--PQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEV 220
            IP + E   E+   QIA  P V VNRV+TYKELD    +H A    + +IDLK+LSN L  +SEV
Sbjct:   43 IPDLEEVQEEDMATQIAAPPSVQVNRVATYKELDSYYQKHSALFSLDGDIDLKLLSNVLSPESEV 107
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Medaka
Match: IFT43 (intraflagellar transport 43 [Source:NCBI gene;Acc:101166174])

HSP 1 Score: 51.9878 bits (123), Expect = 1.936e-9
Identity = 30/71 (42.25%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:   29 DIPTIPEAS--NEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP+     EE    Q+A  P + V++V TY++LD DL  + AF   + EIDLK+L+  L  + EV+E
Sbjct:   98 DIPVIPDLDEVQEEDLTMQVAVPPSIHVSQVMTYRDLDNDLKHYSAFQTLDGEIDLKLLTKVLAPEQEVKE 168
[Close]
BLAST of Intraflagellar transport protein 43 vs. Ensembl Medaka
Match: IFT43 (intraflagellar transport 43 [Source:NCBI gene;Acc:101166174])

HSP 1 Score: 51.6026 bits (122), Expect = 2.166e-9
Identity = 30/71 (42.25%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:   29 DIPTIPEAS--NEEP---QIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            DIP IP+     EE    Q+A  P + V++V TY++LD DL  + AF   + EIDLK+L+  L  + EV+E
Sbjct:   99 DIPVIPDLDEVQEEDLTMQVAVPPSIHVSQVMTYRDLDNDLKHYSAFQTLDGEIDLKLLTKVLAPEQEVKE 169
[Close]
BLAST of Intraflagellar transport protein 43 vs. Planmine SMEST
Match: SMESG000067883.1 (SMESG000067883.1)

HSP 1 Score: 133.65 bits (335), Expect = 1.555e-41
Identity = 64/67 (95.52%), Postives = 66/67 (98.51%), Query Frame = 2 
Query:   26 LDIPTIPEASNEEPQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            ++IPTIPEASNEEPQIAQAP VMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE
Sbjct:   82 MNIPTIPEASNEEPQIAQAPNVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 148
[Close]
BLAST of Intraflagellar transport protein 43 vs. Planmine SMEST
Match: SMESG000067883.1 (SMESG000067883.1)

HSP 1 Score: 133.65 bits (335), Expect = 1.600e-41
Identity = 64/67 (95.52%), Postives = 66/67 (98.51%), Query Frame = 2 
Query:   26 LDIPTIPEASNEEPQIAQAPKVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 226
            ++IPTIPEASNEEPQIAQAP VMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE
Sbjct:   83 MNIPTIPEASNEEPQIAQAPNVMVNRVSTYKELDRDLSEHVAFTVFEDEIDLKILSNCLMLKSEVEE 149
The following BLAST results are available for this feature:
BLAST of Intraflagellar transport protein 43 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
IFT432.603e-942.67intraflagellar transport 43 [Source:HGNC Symbol;Ac... [more]
IFT432.603e-942.67intraflagellar transport 43 [Source:HGNC Symbol;Ac... [more]
IFT436.435e-942.25intraflagellar transport 43 [Source:HGNC Symbol;Ac... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rgs7bp4.018e-1249.30regulator of G-protein signaling 7 binding protein... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ift432.927e-1047.17intraflagellar transport 43 [Source:MGI Symbol;Acc... [more]
Ift433.965e-1047.17intraflagellar transport 43 [Source:MGI Symbol;Acc... [more]
Ift431.841e-948.89intraflagellar transport 43 [Source:MGI Symbol;Acc... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q28CW2|IFT43_XENTR2.129e-1149.30Intraflagellar transport protein 43 homolog OS=Xen... [more]
sp|Q3B8E9|IFT43_XENLA8.042e-1146.48Intraflagellar transport protein 43 homolog OS=Xen... [more]
sp|B5X7E4|IF43B_SALSA9.158e-1144.59Intraflagellar transport protein 43 homolog B OS=S... [more]
sp|E2QSX5|IFT43_CANLF1.147e-1046.48Intraflagellar transport protein 43 homolog OS=Can... [more]
sp|Q2TBN9|IFT43_BOVIN2.347e-1046.48Intraflagellar transport protein 43 homolog OS=Bos... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7U7U59.486e-1446.84Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A1I8HV326.761e-1349.12Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DWM37.172e-1349.12Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A0P4W2M41.929e-1262.26Uncharacterized protein OS=Scylla olivacea OX=8555... [more]
A0A3R7PLZ31.977e-1254.93Putative intraflagellar transport protein 43-like ... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
IFT434.263e-1245.83intraflagellar transport 43 [Source:NCBI gene;Acc:... [more]
IFT436.054e-1245.83intraflagellar transport 43 [Source:NCBI gene;Acc:... [more]
IFT433.002e-1145.07intraflagellar transport 43 [Source:NCBI gene;Acc:... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000008891.12.781e-1240.85pep scaffold:Pmarinus_7.0:GL476825:10523:32821:-1 ... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO422501.538e-1149.23Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
IFT431.936e-942.25intraflagellar transport 43 [Source:NCBI gene;Acc:... [more]
IFT432.166e-942.25intraflagellar transport 43 [Source:NCBI gene;Acc:... [more]
back to top
BLAST of Intraflagellar transport protein 43 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000067883.11.555e-4195.52SMESG000067883.1[more]
SMESG000067883.11.600e-4195.52SMESG000067883.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014550 ID=SMED30014550|Name=Intraflagellar transport protein 43|organism=Schmidtea mediterranea sexual|type=transcript|length=230bp
TTTTAGAGCAGTTACTTATGGAACTTTAGACATTCCAACTATTCCGGAAG
CATCGAATGAAGAACCACAAATCGCGCAAGCCCCAAAGGTTATGGTAAAC
CGGGTATCAACCTACAAGGAATTAGACCGAGATCTCAGTGAACATGTCGC
ATTTACCGTGTTTGAGGACGAGATCGATTTGAAAATCTTATCTAATTGTT
TGATGTTGAAATCTGAAGTTGAAGAGGTAA
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0031514motile cilium
GO:0030991intraciliary transport particle A
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0005929cilium
GO:0042995cell projection
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR029302IFT43
Vocabulary: biological process
TermDefinition
GO:0030030cell projection organization
GO:0035721intraciliary retrograde transport
GO:0060271cilium morphogenesis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029302Intraflagellar transport protein 43PFAMPF15305IFT43coord: 10..75
e-value: 4.6E-15
score: 56.0
IPR029302Intraflagellar transport protein 43PANTHERPTHR33724FAMILY NOT NAMEDcoord: 10..75