Kunitz domain-containing protein

Overview


Name	Kunitz domain-containing protein
Smed ID	SMED30014529
Length (bp)	315

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Kunitz domain-containing protein (SMED30014529) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Kunitz domain-containing protein (SMED30014529) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30014529

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
nervous system	SMED30014529	dd_Smed_v4_8055_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30014529	dd_Smed_v4_8055_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30014529	dd_Smed_v4_8055_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 62.003 bits (149), Expect = 4.978e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++ S  KC  F YGGC  N NNF +   C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 62.003 bits (149), Expect = 4.984e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++ S  KC  F YGGC  N NNF +   C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 62.003 bits (149), Expect = 4.984e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++ S  KC  F YGGC  N NNF +   C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 61.6178 bits (148), Expect = 6.355e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++ S  KC  F YGGC  N NNF +   C   C+
Sbjct:  320 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 371

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Human
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:HGNC Symbol;Acc:HGNC:30916])

HSP 1 Score: 57.7658 bits (138), Expect = 1.526e-10
Identity = 24/57 (42.11%), Postives = 31/57 (54.39%), Query Frame = 1

Query:   49 SSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            S  +  C  P   GPC+A  PR+ YN  T +C+ F YGGC  N NNF + + C   C
Sbjct:  287 SGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESC 343

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Celegans
Match: mig-6 (Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840])

HSP 1 Score: 56.225 bits (134), Expect = 1.982e-10
Identity = 25/51 (49.02%), Postives = 31/51 (60.78%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC      YYYN ++ +CE FTYGGC  N N F TI+ C+ +C
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARC 1554

HSP 2 Score: 45.4394 bits (106), Expect = 1.386e-6
Identity = 23/69 (33.33%), Postives = 36/69 (52.17%), Query Frame = 1

Query:   43 LASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFM 249
            L S+    C   +  GPC      +Y++ +   C+ FTYGGCR N+N FV+ + C+  C   + K E +
Sbjct: 1845 LWSNNTDFCTLERSAGPCTDSISMWYFDSTHLDCKPFTYGGCRGNQNRFVSKEQCQQSCRPGDTKSEDI 1913

HSP 3 Score: 44.669 bits (104), Expect = 2.147e-6
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C + K TGPC     ++YYN++   C  F YGGC+   N F   + C+  C+
Sbjct: 1447 CDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQ 1498

HSP 4 Score: 44.2838 bits (103), Expect = 3.811e-6
Identity = 25/95 (26.32%), Postives = 48/95 (50.53%), Query Frame = 1

Query:   25 LILSFHLASSRVPGCFQPKFTGP-CRAGFP-RYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFMYDCFILRIMK*LHCLSIK 303
             ++   +A  ++  C Q + +G  C AG+   ++Y+ +  +C +F YGGC  N+NNF +  +C   C +   K      C++ R+   L C  ++
Sbjct: 1075 FLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVEPPGK----GRCYLPRVDGPLRCDQLQ 1165

[Close]

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC      YYYN ++ +CE FTYGGC  N N F TI+ C+ +C
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARC 1554

HSP 2 Score: 45.4394 bits (106), Expect = 1.386e-6
Identity = 23/69 (33.33%), Postives = 36/69 (52.17%), Query Frame = 1

Query:   43 LASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFM 249
            L S+    C   +  GPC      +Y++ +   C+ FTYGGCR N+N FV+ + C+  C   + K E +
Sbjct: 1845 LWSNNTDFCTLERSAGPCTDSISMWYFDSTHLDCKPFTYGGCRGNQNRFVSKEQCQQSCRPGDTKSEDI 1913

HSP 3 Score: 44.669 bits (104), Expect = 2.147e-6
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C + K TGPC     ++YYN++   C  F YGGC+   N F   + C+  C+
Sbjct: 1447 CDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQ 1498

HSP 4 Score: 44.2838 bits (103), Expect = 3.811e-6
Identity = 25/95 (26.32%), Postives = 48/95 (50.53%), Query Frame = 1

Query:   25 LILSFHLASSRVPGCFQPKFTGP-CRAGFP-RYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFMYDCFILRIMK*LHCLSIK 303
             ++   +A  ++  C Q + +G  C AG+   ++Y+ +  +C +F YGGC  N+NNF +  +C   C +   K      C++ R+   L C  ++
Sbjct: 1075 FLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVEPPGK----GRCYLPRVDGPLRCDQLQ 1165

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Celegans
Match: mig-6 (Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840])

HSP 1 Score: 55.8398 bits (133), Expect = 2.841e-10
Identity = 25/51 (49.02%), Postives = 31/51 (60.78%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC      YYYN ++ +CE FTYGGC  N N F TI+ C+ +C
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARC 1554

HSP 2 Score: 44.2838 bits (103), Expect = 2.875e-6
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C + K TGPC     ++YYN++   C  F YGGC+   N F   + C+  C+
Sbjct: 1447 CDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQ 1498

HSP 3 Score: 43.8986 bits (102), Expect = 4.002e-6
Identity = 25/95 (26.32%), Postives = 48/95 (50.53%), Query Frame = 1

Query:   25 LILSFHLASSRVPGCFQPKFTGP-CRAGFP-RYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFMYDCFILRIMK*LHCLSIK 303
             ++   +A  ++  C Q + +G  C AG+   ++Y+ +  +C +F YGGC  N+NNF +  +C   C +   K      C++ R+   L C  ++
Sbjct: 1075 FLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVEPPGK----GRCYLPRVDGPLRCDQLQ 1165

[Close]

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC      YYYN ++ +CE FTYGGC  N N F TI+ C+ +C
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARC 1554

HSP 2 Score: 44.2838 bits (103), Expect = 2.875e-6
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C + K TGPC     ++YYN++   C  F YGGC+   N F   + C+  C+
Sbjct: 1447 CDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQ 1498

HSP 3 Score: 43.8986 bits (102), Expect = 4.002e-6
Identity = 25/95 (26.32%), Postives = 48/95 (50.53%), Query Frame = 1

Query:   25 LILSFHLASSRVPGCFQPKFTGP-CRAGFP-RYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFMYDCFILRIMK*LHCLSIK 303
             ++   +A  ++  C Q + +G  C AG+   ++Y+ +  +C +F YGGC  N+NNF +  +C   C +   K      C++ R+   L C  ++
Sbjct: 1075 FLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVEPPGK----GRCYLPRVDGPLRCDQLQ 1165

[Close]

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC      YYYN ++ +CE FTYGGC  N N F TI+ C+ +C
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARC 1554

HSP 2 Score: 44.2838 bits (103), Expect = 2.875e-6
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C + K TGPC     ++YYN++   C  F YGGC+   N F   + C+  C+
Sbjct: 1447 CDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQ 1498

HSP 3 Score: 43.8986 bits (102), Expect = 4.002e-6
Identity = 25/95 (26.32%), Postives = 48/95 (50.53%), Query Frame = 1

Query:   25 LILSFHLASSRVPGCFQPKFTGP-CRAGFP-RYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK*EFMYDCFILRIMK*LHCLSIK 303
             ++   +A  ++  C Q + +G  C AG+   ++Y+ +  +C +F YGGC  N+NNF +  +C   C +   K      C++ R+   L C  ++
Sbjct: 1075 FLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVEPPGK----GRCYLPRVDGPLRCDQLQ 1165

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0300337)

HSP 1 Score: 63.1586 bits (152), Expect = 1.001e-12
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 1

Query:   10 FISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            F S+        AS  +P C QP  +GPC   F R+YY+  T  C  FTYGGC+ N+NN+ T   C   C Q
Sbjct: 1711 FDSLEQCQGTCAASENLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 44.669 bits (104), Expect = 2.945e-6
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC     ++Y++    +CEEF YGGC    N F +++ C+  C
Sbjct: 1671 CLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC 1721

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301838)

HSP 1 Score: 63.1586 bits (152), Expect = 1.164e-12
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 1

Query:   10 FISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            F S+        AS  +P C QP  +GPC   F R+YY+  T  C  FTYGGC+ N+NN+ T   C   C Q
Sbjct: 1711 FDSLEQCQGTCAASENLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 45.8246 bits (107), Expect = 1.164e-6
Identity = 23/60 (38.33%), Postives = 33/60 (55.00%), Query Frame = 1

Query:   55 RVPG-----CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            R PG     C  PK TG C     ++++++S K+C  F Y GC  N+NNF T++ C   C
Sbjct: 1781 RQPGVLKDRCALPKQTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHC 1840

HSP 3 Score: 44.669 bits (104), Expect = 2.932e-6
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC     ++Y++    +CEEF YGGC    N F +++ C+  C
Sbjct: 1671 CLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC 1721

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301839)

HSP 1 Score: 63.1586 bits (152), Expect = 1.165e-12
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 1

Query:   10 FISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            F S+        AS  +P C QP  +GPC   F R+YY+  T  C  FTYGGC+ N+NN+ T   C   C Q
Sbjct: 1711 FDSLEQCQGTCAASENLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 46.2098 bits (108), Expect = 8.529e-7
Identity = 21/51 (41.18%), Postives = 29/51 (56.86%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C +P  TGPC     +YY+N +++ CE FTYGGC    N F  +  C+  C
Sbjct: 2196 CNEPVTTGPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVC 2246

HSP 3 Score: 44.669 bits (104), Expect = 3.020e-6
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC     ++Y++    +CEEF YGGC    N F +++ C+  C
Sbjct: 1671 CLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC 1721

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301837)

HSP 1 Score: 63.1586 bits (152), Expect = 1.165e-12
Identity = 30/72 (41.67%), Postives = 38/72 (52.78%), Query Frame = 1

Query:   10 FISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            F S+        AS  +P C QP  +GPC   F R+YY+  T  C  FTYGGC+ N+NN+ T   C   C Q
Sbjct: 1711 FDSLEQCQGTCAASENLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 2 Score: 46.2098 bits (108), Expect = 8.700e-7
Identity = 21/51 (41.18%), Postives = 29/51 (56.86%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C +P  TGPC     +YY+N +++ CE FTYGGC    N F  +  C+  C
Sbjct: 2253 CNEPVTTGPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVC 2303

HSP 3 Score: 45.8246 bits (107), Expect = 1.247e-6
Identity = 23/60 (38.33%), Postives = 33/60 (55.00%), Query Frame = 1

Query:   55 RVPG-----CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            R PG     C  PK TG C     ++++++S K+C  F Y GC  N+NNF T++ C   C
Sbjct: 1781 RQPGVLKDRCALPKQTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHC 1840

HSP 4 Score: 44.669 bits (104), Expect = 2.992e-6
Identity = 19/51 (37.25%), Postives = 28/51 (54.90%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPC     ++Y++    +CEEF YGGC    N F +++ C+  C
Sbjct: 1671 CLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC 1721

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Fly
Match: CG10031 (gene:FBgn0031563 transcript:FBtr0077544)

HSP 1 Score: 59.3066 bits (142), Expect = 2.364e-12
Identity = 25/53 (47.17%), Postives = 31/53 (58.49%), Query Frame = 1

Query:   61 PGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            P C QP   GPCR    R+YYN+ +K CE F YGGCR N+N +   + C   C
Sbjct:   73 PKCLQPLDVGPCRMSLERFYYNKDSKACETFKYGGCRGNDNRWGFRQTCEEAC 125

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 66.2402 bits (160), Expect = 9.606e-14
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  PK  GPCR  FPR++YN +T+KCEEF +GGC  N NN++ +  C+  C +
Sbjct:  247 CLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 299

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 66.2402 bits (160), Expect = 1.036e-13
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  PK  GPCR  FPR++YN +T+KCEEF +GGC  N NN++ +  C+  C +
Sbjct:  239 CLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 291

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 66.2402 bits (160), Expect = 1.036e-13
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  PK  GPCR  FPR++YN +T+KCEEF +GGC  N NN++ +  C+  C +
Sbjct:  239 CLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 291

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 a [Source:ZFIN;Acc:ZDB-GENE-040426-2169])

HSP 1 Score: 66.2402 bits (160), Expect = 1.171e-13
Identity = 26/53 (49.06%), Postives = 36/53 (67.92%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  PK  GPCR  FPR++YN +T+KCEEF +GGC  N NN++ +  C+  C +
Sbjct:  239 CLVPKKEGPCRGSFPRWHYNAATEKCEEFKFGGCVPNRNNYLALNECQSACNK 291

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BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
Match: spint1b (serine peptidase inhibitor, Kunitz type 1 b [Source:ZFIN;Acc:ZDB-GENE-071218-1])

HSP 1 Score: 62.7734 bits (151), Expect = 1.701e-12
Identity = 25/52 (48.08%), Postives = 37/52 (71.15%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C  PK TGPCRA F R+ YN ++++CE+F +GGC +N NN+++   C+  CE
Sbjct:  235 CLTPKKTGPCRASFIRWNYNAASRRCEQFIFGGCMENSNNYLSETECQNACE 286

HSP 2 Score: 60.077 bits (144), Expect = 1.300e-11
Identity = 28/65 (43.08%), Postives = 35/65 (53.85%), Query Frame = 1

Query:   25 LILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            L    ++  ++   C  P  TGPCRA F  +YY+  +KKC  FTYGGC  N NNF T   C   C
Sbjct:  349 LTRLLNIEVNKKAHCTDPPATGPCRAHFHHWYYDPLSKKCHSFTYGGCDGNRNNFETADKCMKNC 413

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
Match: rlbp1 (retinaldehyde binding protein 1 [Source:Xenbase;Acc:XB-GENE-976303])

HSP 1 Score: 65.4698 bits (158), Expect = 2.608e-13
Identity = 26/51 (50.98%), Postives = 33/51 (64.71%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            CF P   G CR  F R+Y+N  T  C EFTYGGC+ N+NN++ I+ CR  C
Sbjct:  240 CFAPHKVGRCRGSFTRWYFNPETNDCLEFTYGGCKPNKNNYLRIEDCRQTC 290

HSP 2 Score: 58.9214 bits (141), Expect = 4.895e-11
Identity = 26/52 (50.00%), Postives = 31/52 (59.62%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C     TGPCRA F R+YY+ + +KC  FTYGGC  N+NNF     C   CE
Sbjct:  358 CVDLPDTGPCRASFSRFYYDPNIRKCLRFTYGGCAGNDNNFHRESDCMRYCE 409

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
Match: spint2 (serine peptidase inhibitor, Kunitz type, 2 [Source:NCBI gene;Acc:733874])

HSP 1 Score: 64.6994 bits (156), Expect = 4.289e-13
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  P  TGPCRA F R+YY+ +T  C  FTYGGCR N+NN+++++ C   C
Sbjct:  268 CAAPSLTGPCRASFRRWYYDVTTATCVAFTYGGCRGNKNNYLSVEDCVKNC 318

HSP 2 Score: 61.6178 bits (148), Expect = 6.252e-12
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C     TGPCRA F R++YN +T+ CE FTYGGC+ N NN +  ++C  +C
Sbjct:  119 CLPEAVTGPCRAAFERWWYNPNTQTCENFTYGGCKGNLNNHIGEEVCMNKC 169

HSP 3 Score: 53.9138 bits (128), Expect = 2.664e-9
Identity = 22/53 (41.51%), Postives = 31/53 (58.49%), Query Frame = 1

Query:   61 PGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            P C      G CRA FPR+Y++  ++ C  FTYGGC   ENN  +++ C  +C
Sbjct:  194 PACTGKGVIGNCRASFPRWYFDAESQNCISFTYGGCGGTENNHKSVQECADRC 246

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 63.1586 bits (152), Expect = 2.069e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++   KKC  F YGGC  N NNF +   C   C+
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 63.1586 bits (152), Expect = 2.071e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++   KKC  F YGGC  N NNF +   C   C+
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 63.1586 bits (152), Expect = 2.073e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++   KKC  F YGGC  N NNF +   C   C+
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Mouse
Match: Spint3 (serine peptidase inhibitor, Kunitz type, 3 [Source:MGI Symbol;Acc:MGI:3651470])

HSP 1 Score: 60.4622 bits (145), Expect = 4.853e-13
Identity = 30/56 (53.57%), Postives = 34/56 (60.71%), Query Frame = 1

Query:   52 SRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            S  P C  PK  G CRA F R+YYN  T KCE F YGGC+ NENNF +   C+  C
Sbjct:   30 SLPPMCILPKDIGGCRAVFVRWYYNSKTGKCEWFRYGGCKGNENNFPSRSQCQAVC 85

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 62.003 bits (149), Expect = 3.418e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++ S  KC  F YGGC  N NNF +   C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 61.6178 bits (148), Expect = 5.167e-12
Identity = 25/52 (48.08%), Postives = 30/52 (57.69%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            C Q   TGPCRA  PR+Y++ S  KC  F YGGC  N NNF +   C   C+
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Mouse
Match: Wfdc6a (WAP four-disulfide core domain 6A [Source:MGI Symbol;Acc:MGI:2684968])

HSP 1 Score: 58.5362 bits (140), Expect = 7.518e-12
Identity = 26/53 (49.06%), Postives = 34/53 (64.15%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  P+  GPC A  PR++YNQ T  C EF YGGC+ N NNF +  +C V C++
Sbjct:   77 CSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGICTVVCKK 129

[Close]

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  P+  GPC A  PR++YNQ T  C EF YGGC+ N NNF +  +C V C++
Sbjct:   77 CSLPQDPGPCLAYLPRWWYNQETDLCTEFIYGGCQGNPNNFPSEGICTVVCKK 129

[Close]

BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DN09|VKT5_ANEVI (U-actitoxin-Avd3h OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.144e-16
Identity = 32/51 (62.75%), Postives = 37/51 (72.55%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCRA FPRYYYN S+K+CE+F YGGCR N NNF T++ C   C
Sbjct:   21 CELPKVVGPCRARFPRYYYNSSSKRCEKFIYGGCRGNANNFHTLEECEKVC 71

[Close]

BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DN10|VKT6_ANEVI (U-actitoxin-Avd3i OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.422e-16
Identity = 32/51 (62.75%), Postives = 37/51 (72.55%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCRAGF RYYYN S+K+CE+F YGGCR N NNF T++ C   C
Sbjct:   21 CELPKVVGPCRAGFRRYYYNSSSKRCEKFIYGGCRGNANNFHTLEECEKVC 71

[Close]

BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DN17|VKTD_ANEVI (U-actitoxin-Avd3p OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.765e-16
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCRA  PRYYYN S+K+CE+F YGGCR N NNF T++ C   C
Sbjct:   21 CELPKVVGPCRASHPRYYYNSSSKRCEKFNYGGCRGNANNFHTLEECEKVC 71

[Close]

BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
Match: sp|P0DN11|VKT7_ANEVI (U-actitoxin-Avd3j OS=Anemonia viridis OX=51769 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.151e-16
Identity = 31/51 (60.78%), Postives = 36/51 (70.59%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCRA  PRYYYN S+K+CE+F YGGCR N NNF T++ C   C
Sbjct:   21 CELPKVVGPCRASHPRYYYNSSSKRCEKFIYGGCRGNANNFHTLEECEKVC 71

[Close]

BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6T6S5|VKT2_BITGA (Kunitz-type serine protease inhibitor bitisilin-2 OS=Bitis gabonica OX=8694 PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.550e-16
Identity = 32/66 (48.48%), Postives = 42/66 (63.64%), Query Frame = 1

Query:   22 WLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            W  L+      R   C+ P  TGPC A FPR+YY+ ++KKC++FTYGGC  N NNF T + CR +C
Sbjct:   16 WAELTPVSGKKRPDFCYLPADTGPCMANFPRFYYDSASKKCKKFTYGGCHGNANNFETREECRKKC 81

[Close]

BLAST of Kunitz domain-containing protein vs. TrEMBL
Match: A0A5B2VB79 (BPTI/Kunitz inhibitor domain-containing protein (Fragment) OS=Chitinophaga sp. BN140078 OX=2607653 GN=F0L74_32960 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.382e-15
Identity = 36/72 (50.00%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 ILFISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            I F+ + L+ SF  A+   P C  P  TGPCR  FPRY Y+ S  KC EF YGGC+ N NNF TI+ C   C
Sbjct:    7 ISFLLIILLFSFTNAN---PDCLLPIKTGPCRGSFPRYAYDSSEDKCVEFIYGGCQANANNFETIEECEAAC 75

[Close]

BLAST of Kunitz domain-containing protein vs. TrEMBL
Match: E2BB27 (Four-domain proteases inhibitor OS=Harpegnathos saltator OX=610380 GN=EAI_06795 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.467e-15
Identity = 32/64 (50.00%), Postives = 42/64 (65.62%), Query Frame = 1

Query:   28 ILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            ILS  + +     C  PK  GPCRA   RY Y+ +T +CEEFTYGGC+ NENNF+T ++C+  C
Sbjct:   16 ILSHEIVAKPSSICQLPKVVGPCRASLKRYRYDSTTGQCEEFTYGGCKGNENNFITREVCQENC 79

[Close]

BLAST of Kunitz domain-containing protein vs. TrEMBL
Match: Q8T7L9 (Kazal-type serine proteinase inhibitor 1 OS=Bombyx mori OX=7091 GN=SPI1 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.087e-14
Identity = 35/72 (48.61%), Postives = 43/72 (59.72%), Query Frame = 1

Query:    4 ILFISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            I F+ + L+ SF  A+   P C  P  TGPC+  FPRY Y+ S  KC EF YGGC+ N NNF TI+ C   C
Sbjct:    7 ISFLLIILLFSFTNAN---PDCLLPIKTGPCKGSFPRYAYDSSEDKCVEFIYGGCQANANNFETIEECEAAC 75

[Close]

BLAST of Kunitz domain-containing protein vs. TrEMBL
Match: A0A1D2MN35 (Kunitz-type serine protease inhibitor kalicludine-1 OS=Orchesella cincta OX=48709 GN=Ocin01_12432 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.580e-14
Identity = 35/75 (46.67%), Postives = 46/75 (61.33%), Query Frame = 1

Query:    4 ILFISVWLIL---SFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            +L I+  LIL   +   A++    C QP   G CRA   R+YYN  TKKCE+F YGGC+KN+NNF T++ C   C
Sbjct:    6 VLAIAAMLILFCANSASAAALEDSCQQPMERGTCRAMHARFYYNSETKKCEKFNYGGCKKNQNNFKTMEECEKAC 80

[Close]

BLAST of Kunitz domain-containing protein vs. TrEMBL
Match: A0A151HYY2 (Taicatoxin, serine protease inhibitor component OS=Atta colombica OX=520822 GN=ALC53_12607 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.820e-14
Identity = 34/75 (45.33%), Postives = 47/75 (62.67%), Query Frame = 1

Query:    4 ILFISVWLILSFHLASSRV-PG-CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            +LF+ + +IL   + S ++ P  C  P  TGPCRA  PRY YN ++ +CE+F YGGC  N NNF TI  C + C+
Sbjct:   11 LLFVFIIVILMHEITSKQLYPSLCLLPMNTGPCRAQIPRYAYNSASGQCEQFIYGGCHANANNFKTISECEMTCK 85

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:103032340])

HSP 1 Score: 68.5514 bits (166), Expect = 1.252e-14
Identity = 26/53 (49.06%), Postives = 40/53 (75.47%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  PK  GPCR  FPR++YN +++KCEEFT+GGC++N NN+++ + C   C++
Sbjct:  237 CLVPKKAGPCRGSFPRWHYNAASEKCEEFTFGGCKENRNNYLSREECLNACDK 289

HSP 2 Score: 59.6918 bits (143), Expect = 1.566e-11
Identity = 26/51 (50.98%), Postives = 31/51 (60.78%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C QP  TGPCRA F ++YYN   +KC  F YGGC+ N+N F T   C   C
Sbjct:  371 CTQPPVTGPCRASFTKWYYNPYDRKCNRFNYGGCQGNDNQFETQDHCMKVC 421

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
Match: spint1b (kunitz-type protease inhibitor 1-like [Source:NCBI gene;Acc:103024868])

HSP 1 Score: 68.5514 bits (166), Expect = 1.390e-14
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCR  FPR++YN ++ KCEEFT+GGC +N NN+++   C   C
Sbjct:  235 CLTPKKVGPCRGAFPRWHYNAASNKCEEFTFGGCLENGNNYLSSDDCVDAC 285

HSP 2 Score: 60.8474 bits (146), Expect = 7.483e-12
Identity = 26/57 (45.61%), Postives = 31/57 (54.39%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK 237
            C  P  TGPCRA F R+YY+   + C  FTYGGC  N+NNF     C   C    +K
Sbjct:  334 CVDPPVTGPCRASFTRWYYDPLQQDCHRFTYGGCTGNKNNFEGQDKCMDACRDVTEK 390

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:103032340])

HSP 1 Score: 68.5514 bits (166), Expect = 1.472e-14
Identity = 26/53 (49.06%), Postives = 40/53 (75.47%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C  PK  GPCR  FPR++YN +++KCEEFT+GGC++N NN+++ + C   C++
Sbjct:  237 CLVPKKAGPCRGSFPRWHYNAASEKCEEFTFGGCKENRNNYLSREECLNACDK 289

HSP 2 Score: 59.6918 bits (143), Expect = 1.738e-11
Identity = 26/51 (50.98%), Postives = 31/51 (60.78%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C QP  TGPCRA F ++YYN   +KC  F YGGC+ N+N F T   C   C
Sbjct:  358 CTQPPVTGPCRASFTKWYYNPYDRKCNRFNYGGCQGNDNQFETQDHCMKVC 408

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
Match: lrp11 (LDL receptor related protein 11 [Source:NCBI gene;Acc:103032685])

HSP 1 Score: 65.0846 bits (157), Expect = 1.956e-13
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C +P   GPCR  FPR+YY+ +  +C+ F YGGC+ N NNF+    C  +C Q
Sbjct:  417 CMEPPVVGPCRGTFPRWYYDAAAGECKHFLYGGCKGNRNNFLQQTDCVNECIQ 469

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
Match: lrp11 (LDL receptor related protein 11 [Source:NCBI gene;Acc:103032685])

HSP 1 Score: 64.3142 bits (155), Expect = 3.593e-13
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C +P   GPCR  FPR+YY+ +  +C+ F YGGC+ N NNF+    C  +C Q
Sbjct:  417 CMEPPVVGPCRGTFPRWYYDAAAGECKHFLYGGCKGNRNNFLQQTDCVNECIQ 469

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 68.9366 bits (167), Expect = 6.276e-16
Identity = 33/61 (54.10%), Postives = 39/61 (63.93%), Query Frame = 1

Query:   37 FHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            F L   RV  C  P   G CRA FPRY++N+ST +CE F YGGC  NENNF T++ CR  C
Sbjct:   18 FTLEDQRVT-CTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAAC 77

HSP 2 Score: 46.9802 bits (110), Expect = 7.594e-8
Identity = 21/51 (41.18%), Postives = 27/51 (52.94%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C     TG C+  F R+YYN   + C  F+Y GC  N NNF++ K C   C
Sbjct:  147 CLSRHDTGNCQGNFRRFYYNIQKRSCLPFSYSGCGGNNNNFISAKTCLSTC 197

HSP 3 Score: 43.1282 bits (100), Expect = 1.734e-6
Identity = 22/56 (39.29%), Postives = 29/56 (51.79%), Query Frame = 1

Query:   55 RVPGCFQPKF-TGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            ++P   +P   TG CRA   RY+YN +   C  F YGGC  N N F  ++ C   C
Sbjct:   82 KIPKLCRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESCVFSC 137

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009465.1 (pep scaffold:Pmarinus_7.0:GL478507:22936:25713:-1 gene:ENSPMAG00000008558.1 transcript:ENSPMAT00000009465.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.151 bits (139), Expect = 9.131e-12
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C     TGPCR  F R+ Y+ +T  C+EFTYGGCR N+NNF+    C + C
Sbjct:    1 CLLNPTTGPCRGSFTRWTYDAATSTCKEFTYGGCRGNQNNFLEEAECLMDC 51

HSP 2 Score: 51.9878 bits (123), Expect = 1.265e-9
Identity = 23/51 (45.10%), Postives = 29/51 (56.86%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C   +  GPCR  F R+ Y+  +  C+EFTYGGC  N NNF+    C V C
Sbjct:   72 CLLEEAVGPCRGSFTRWIYDAESSNCKEFTYGGCGGNFNNFLEETECMVTC 122

HSP 3 Score: 50.0618 bits (118), Expect = 6.347e-9
Identity = 27/72 (37.50%), Postives = 34/72 (47.22%), Query Frame = 1

Query:    7 LFISVWLILS-FHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            L  S W+    FH+       C     T PCR  F R+ Y+  +  C+EFTYGGC  N NNF+    C   C
Sbjct:  149 LISSCWMTRRLFHVQDV----CLLEAVTSPCRGSFTRWTYDAESGHCKEFTYGGCGGNFNNFLEETECVAMC 216

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 3.807e-10
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C      GPCRA  PR +YN+ T++CE F YGGC  N NNF ++  C  +C
Sbjct:    3 CMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

HSP 2 Score: 51.9878 bits (123), Expect = 1.212e-9
Identity = 24/53 (45.28%), Postives = 29/53 (54.72%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C      G C A  PR++YN  T  CE FTYGGC  N+NNF + + C   C Q
Sbjct:  166 CLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVCGQ 218

HSP 3 Score: 45.0542 bits (105), Expect = 3.208e-7
Identity = 21/45 (46.67%), Postives = 26/45 (57.78%), Query Frame = 1

Query:   85 TGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
             G CRA   RY++N ++  CEEF YGGC+ N NNF     C   C
Sbjct:   80 VGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 124

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 3.876e-10
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C      GPCRA  PR +YN+ T++CE F YGGC  N NNF ++  C  +C
Sbjct:    3 CMMDLDPGPCRAYLPRVFYNRYTQRCEPFHYGGCFGNGNNFESLAECNAKC 53

HSP 2 Score: 51.9878 bits (123), Expect = 1.297e-9
Identity = 24/53 (45.28%), Postives = 29/53 (54.72%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C      G C A  PR++YN  T  CE FTYGGC  N+NNF + + C   C Q
Sbjct:  167 CLMKADPGECTAVIPRFHYNPKTSNCESFTYGGCGGNDNNFSSERSCMSVCGQ 219

HSP 3 Score: 45.4394 bits (106), Expect = 3.005e-7
Identity = 21/45 (46.67%), Postives = 26/45 (57.78%), Query Frame = 1

Query:   85 TGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
             G CRA   RY++N ++  CEEF YGGC+ N NNF     C   C
Sbjct:   81 VGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 125

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 50.447 bits (119), Expect = 7.067e-9
Identity = 23/51 (45.10%), Postives = 29/51 (56.86%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  P   GPC+    R+ YN   K+C+ F YGGC  NENNF + +LC   C
Sbjct:  374 CSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCEDNC 424

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 66.6254 bits (161), Expect = 2.098e-16
Identity = 28/51 (54.90%), Postives = 38/51 (74.51%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C QPK TGPCRA F R++YNQ+++KC++F YGGC+ N NNF +   C  +C
Sbjct:    2 CLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 66.6254 bits (161), Expect = 2.098e-16
Identity = 28/51 (54.90%), Postives = 38/51 (74.51%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C QPK TGPCRA F R++YNQ+++KC++F YGGC+ N NNF +   C  +C
Sbjct:    2 CLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
Match: EDO29819 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2J6])

HSP 1 Score: 60.077 bits (144), Expect = 1.200e-13
Identity = 27/58 (46.55%), Postives = 34/58 (58.62%), Query Frame = 1

Query:   46 ASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            A S    C  PK  G CRA  PR ++N  + KCE+F YGGC  N NNF T++ C+  C
Sbjct:    7 AVSGNSACTLPKKVGDCRAAMPRVFFNSQSGKCEDFIYGGCGGNANNFHTMEECKKAC 64

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
Match: EDO38284 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDB9])

HSP 1 Score: 58.9214 bits (141), Expect = 2.888e-13
Identity = 26/51 (50.98%), Postives = 30/51 (58.82%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PKF GPC A   R+YYN    KC+ F +GGC  N NNF T K C  +C
Sbjct:   13 CKLPKFAGPCMASIERFYYNAKIGKCQSFVFGGCLPNGNNFKTRKACEAKC 63

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
Match: EDO38466 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCV3])

HSP 1 Score: 57.7658 bits (138), Expect = 1.089e-12
Identity = 27/65 (41.54%), Postives = 40/65 (61.54%), Query Frame = 1

Query:   28 ILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCE 222
            +L FH +++    C  PK TGPC A F R++Y+  + +C+ FTYGGC+ N NNF +   C  +C 
Sbjct:    3 LLIFH-SNTATQVCSLPKLTGPCMARFIRWHYDMRSSECKMFTYGGCQGNANNFESKAACLKKCS 66

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
Match: spint1a (kunitz-type protease inhibitor 1 [Source:NCBI gene;Acc:101175207])

HSP 1 Score: 67.781 bits (164), Expect = 2.753e-14
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCR  FPR+YYN +++KCE F +GGCR+N+NN++T + C + C
Sbjct:  234 CLVPKKVGPCRGSFPRWYYNAASEKCETFFFGGCRQNKNNYLTEEECAMAC 284

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:101173105])

HSP 1 Score: 65.855 bits (159), Expect = 1.034e-13
Identity = 25/51 (49.02%), Postives = 37/51 (72.55%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCR  FPR++YN +++KCE+F +GGCR+N NN++  + C+  C
Sbjct:  239 CMAPKKVGPCRGSFPRWHYNAASQKCEQFIFGGCRENRNNYLLEEECKFAC 289

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
Match: spint1a (serine peptidase inhibitor, Kunitz type 1 [Source:NCBI gene;Acc:101173105])

HSP 1 Score: 65.855 bits (159), Expect = 1.078e-13
Identity = 25/51 (49.02%), Postives = 37/51 (72.55%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            C  PK  GPCR  FPR++YN +++KCE+F +GGCR+N NN++  + C+  C
Sbjct:  239 CMAPKKVGPCRGSFPRWHYNAASQKCEQFIFGGCRENRNNYLLEEECKFAC 289

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
Match: lrp11 (low-density lipoprotein receptor-related protein 11 [Source:NCBI gene;Acc:101175213])

HSP 1 Score: 60.8474 bits (146), Expect = 7.068e-12
Identity = 24/53 (45.28%), Postives = 33/53 (62.26%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C      GPC+  FPR+YY+Q+T +C+ F YGGC+ N NNF+    C  +C Q
Sbjct:  394 CGADPVVGPCKGTFPRWYYDQNTGECKHFLYGGCQGNHNNFLQELDCISECIQ 446

[Close]

BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000018112.2 (tissue factor pathway inhibitor [Source:NCBI gene;Acc:101174546])

HSP 1 Score: 59.6918 bits (143), Expect = 1.636e-11
Identity = 34/83 (40.96%), Postives = 42/83 (50.60%), Query Frame = 1

Query:    7 LFISVWLILSFHLASSRVPG------------CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            +F ++ L    HL SSR               C      GPCRA   RY++N ST +CEEF YGGC  N NNFVT++ C   C
Sbjct:    8 IFCALSLASLAHLGSSRKHRAHGQQPIIFNELCAMKADPGPCRAIKDRYFFNVSTARCEEFEYGGCSGNSNNFVTLQECEETC 90

HSP 2 Score: 48.9062 bits (115), Expect = 8.955e-8
Identity = 20/53 (37.74%), Postives = 31/53 (58.49%), Query Frame = 1

Query:   67 CFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            C   +  GPCR    RY+++  T++C  F YGGC  N NNF ++  C+ +C+ 
Sbjct:   99 CHLEEAPGPCRGLLSRYFFDSKTRQCRRFFYGGCFGNANNFWSMAECQAKCQN 151

[Close]

BLAST of Kunitz domain-containing protein vs. Planmine SMEST
Match: SMESG000013917.1 (SMESG000013917.1)

HSP 1 Score: 155.606 bits (392), Expect = 4.872e-51
Identity = 74/75 (98.67%), Postives = 74/75 (98.67%), Query Frame = 1

Query:    1 FILFISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            FILFISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNE NFVTIKLCRVQCEQ
Sbjct:    3 FILFISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNEKNFVTIKLCRVQCEQ 77

[Close]

BLAST of Kunitz domain-containing protein vs. Planmine SMEST
Match: SMESG000013910.1 (SMESG000013910.1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.447e-19
Identity = 35/70 (50.00%), Postives = 45/70 (64.29%), Query Frame = 1

Query:   16 SVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ 225
            SV   ++ HL   + P C  PK  G CR  FPRY++N+ T KCE+F YGGC+ N+NNF T   CR +CEQ
Sbjct:    3 SVIFAVAIHLGDCKEPECSLPKEVGLCRGYFPRYFFNEETGKCEKFIYGGCQGNKNNFYTPIECRARCEQ 72

[Close]

BLAST of Kunitz domain-containing protein vs. Planmine SMEST
Match: SMESG000038002.1 (SMESG000038002.1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.830e-18
Identity = 37/79 (46.84%), Postives = 47/79 (59.49%), Query Frame = 1

Query:    7 LFISVWLILSFHL--ASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK 237
            L I + L+LSF +    S  P C  P   G C A FPRY+YN +++KCE F YGGC  N NNF T+K C+  CE+ N  
Sbjct:    5 LIICLALVLSFIIFTIESLEPNCEDPLVKGLCEAYFPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLCEETNSD 83

[Close]

BLAST of Kunitz domain-containing protein vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 72.0182 bits (175), Expect = 6.036e-18
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 1

Query:    4 ILFISVWLILSFHLASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQC 219
            I+  ++   + F L  SR P C +PK TG C A FPR+Y+N  +  CE F YGGC+ N NNF TI+ C  +C
Sbjct:    4 IVIFTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 75

[Close]

BLAST of Kunitz domain-containing protein vs. Planmine SMEST
Match: SMESG000037997.1 (SMESG000037997.1)

HSP 1 Score: 72.0182 bits (175), Expect = 6.900e-18
Identity = 37/79 (46.84%), Postives = 47/79 (59.49%), Query Frame = 1

Query:    7 LFISVWLILSFHL--ASSRVPGCFQPKFTGPCRAGFPRYYYNQSTKKCEEFTYGGCRKNENNFVTIKLCRVQCEQ*NKK 237
            L I + L+LSF +    S  P C  P   G C A FPRY+YN +++KCE F YGGC  N NNF T+K C+  CE+ N  
Sbjct:    5 LIIWLALVLSFIIFTIESLEPNCEDPLVKGLCEAYFPRYFYNDTSEKCERFIYGGCGANGNNFKTLKDCQDLCEETNSD 83

The following BLAST results are available for this feature:

BLAST of Kunitz domain-containing protein vs. Ensembl Human
BLAST of Kunitz domain-containing protein vs. Ensembl Celegans
BLAST of Kunitz domain-containing protein vs. Ensembl Fly
BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
BLAST of Kunitz domain-containing protein vs. Ensembl Mouse
BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
BLAST of Kunitz domain-containing protein vs. TrEMBL
BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
BLAST of Kunitz domain-containing protein vs. Ensembl Yeast
BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
BLAST of Kunitz domain-containing protein vs. Planmine SMEST

BLAST of Kunitz domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
APLP2	4.978e-12	48.08	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	4.984e-12	48.08	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	4.984e-12	48.08	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	6.355e-12	48.08	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
WFIKKN2	1.526e-10	42.11	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mig-6	1.982e-10	49.02	Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840]	[more]
mig-6	1.982e-10	49.02	Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840]	[more]
mig-6	2.841e-10	49.02	Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840]	[more]
mig-6	2.841e-10	49.02	Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840]	[more]
mig-6	2.841e-10	49.02	Papilin [Source:UniProtKB/Swiss-Prot;Acc:O76840]	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Ppn	1.001e-12	41.67	gene:FBgn0003137 transcript:FBtr0300337	[more]
Ppn	1.164e-12	41.67	gene:FBgn0003137 transcript:FBtr0301838	[more]
Ppn	1.165e-12	41.67	gene:FBgn0003137 transcript:FBtr0301839	[more]
Ppn	1.165e-12	41.67	gene:FBgn0003137 transcript:FBtr0301837	[more]
CG10031	2.364e-12	47.17	gene:FBgn0031563 transcript:FBtr0077544	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
spint1a	9.606e-14	49.06	serine peptidase inhibitor, Kunitz type 1 a [Sourc...	[more]
spint1a	1.036e-13	49.06	serine peptidase inhibitor, Kunitz type 1 a [Sourc...	[more]
spint1a	1.036e-13	49.06	serine peptidase inhibitor, Kunitz type 1 a [Sourc...	[more]
spint1a	1.171e-13	49.06	serine peptidase inhibitor, Kunitz type 1 a [Sourc...	[more]
spint1b	1.701e-12	48.08	serine peptidase inhibitor, Kunitz type 1 b [Sourc...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
rlbp1	2.608e-13	50.98	retinaldehyde binding protein 1 [Source:Xenbase;Ac...	[more]
spint2	4.289e-13	50.98	serine peptidase inhibitor, Kunitz type, 2 [Source...	[more]
APLP2	2.069e-12	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	2.071e-12	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	2.073e-12	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Spint3	4.853e-13	53.57	serine peptidase inhibitor, Kunitz type, 3 [Source...	[more]
Aplp2	3.418e-12	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
Aplp2	5.167e-12	48.08	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
Wfdc6a	7.518e-12	49.06	WAP four-disulfide core domain 6A [Source:MGI Symb...	[more]
Wfdc6a	7.518e-12	49.06	WAP four-disulfide core domain 6A [Source:MGI Symb...	[more]

BLAST of Kunitz domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P0DN09\|VKT5_ANEVI	1.144e-16	62.75	U-actitoxin-Avd3h OS=Anemonia viridis OX=51769 PE=...	[more]
sp\|P0DN10\|VKT6_ANEVI	1.422e-16	62.75	U-actitoxin-Avd3i OS=Anemonia viridis OX=51769 PE=...	[more]
sp\|P0DN17\|VKTD_ANEVI	2.765e-16	60.78	U-actitoxin-Avd3p OS=Anemonia viridis OX=51769 PE=...	[more]
sp\|P0DN11\|VKT7_ANEVI	3.151e-16	60.78	U-actitoxin-Avd3j OS=Anemonia viridis OX=51769 PE=...	[more]
sp\|Q6T6S5\|VKT2_BITGA	3.550e-16	48.48	Kunitz-type serine protease inhibitor bitisilin-2 ...	[more]

BLAST of Kunitz domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A5B2VB79	5.382e-15	50.00	BPTI/Kunitz inhibitor domain-containing protein (F...	[more]
E2BB27	9.467e-15	50.00	Four-domain proteases inhibitor OS=Harpegnathos sa...	[more]
Q8T7L9	1.087e-14	48.61	Kazal-type serine proteinase inhibitor 1 OS=Bombyx...	[more]
A0A1D2MN35	1.580e-14	46.67	Kunitz-type serine protease inhibitor kalicludine-...	[more]
A0A151HYY2	1.820e-14	45.33	Taicatoxin, serine protease inhibitor component OS...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
spint1a	1.252e-14	49.06	serine peptidase inhibitor, Kunitz type 1 [Source:...	[more]
spint1b	1.390e-14	50.98	kunitz-type protease inhibitor 1-like [Source:NCBI...	[more]
spint1a	1.472e-14	49.06	serine peptidase inhibitor, Kunitz type 1 [Source:...	[more]
lrp11	1.956e-13	45.28	LDL receptor related protein 11 [Source:NCBI gene;...	[more]
lrp11	3.593e-13	45.28	LDL receptor related protein 11 [Source:NCBI gene;...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
tfpi2	6.276e-16	54.10	tissue factor pathway inhibitor 2 [Source:ZFIN;Acc...	[more]
ENSPMAT00000009465.1	9.131e-12	49.02	pep scaffold:Pmarinus_7.0:GL478507:22936:25713:-1 ...	[more]
ENSPMAT00000005043.1	3.807e-10	47.06	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
ENSPMAT00000005038.1	3.876e-10	47.06	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
WFIKKN1	7.067e-9	45.10	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Kunitz domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO41696	2.098e-16	54.90	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26263	2.098e-16	54.90	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO29819	1.200e-13	46.55	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38284	2.888e-13	50.98	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38466	1.089e-12	41.54	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Kunitz domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
spint1a	2.753e-14	52.94	kunitz-type protease inhibitor 1 [Source:NCBI gene...	[more]
spint1a	1.034e-13	49.02	serine peptidase inhibitor, Kunitz type 1 [Source:...	[more]
spint1a	1.078e-13	49.02	serine peptidase inhibitor, Kunitz type 1 [Source:...	[more]
lrp11	7.068e-12	45.28	low-density lipoprotein receptor-related protein 1...	[more]
ENSORLT00000018112.2	1.636e-11	40.96	tissue factor pathway inhibitor [Source:NCBI gene;...	[more]

BLAST of Kunitz domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000013917.1	4.872e-51	98.67	SMESG000013917.1	[more]
SMESG000013910.1	1.447e-19	50.00	SMESG000013910.1	[more]
SMESG000038002.1	3.830e-18	46.84	SMESG000038002.1	[more]
SMESG000037994.1	6.036e-18	45.83	SMESG000037994.1	[more]
SMESG000037997.1	6.900e-18	46.84	SMESG000037997.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30014529 ID=SMED30014529|Name=Kunitz domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=315bp
TTTATATTATTTATTTCAGTTTGGTTAATTTTGTCATTTCATTTAGCCAG
TAGTCGAGTCCCAGGATGTTTCCAACCTAAATTCACTGGACCTTGTCGAG
CTGGTTTTCCTAGATATTATTATAACCAATCAACTAAAAAATGTGAAGAA
TTTACTTATGGAGGATGCCGGAAAAATGAAAATAATTTCGTAACCATTAA
ATTATGCCGTGTTCAATGTGAACAATAAAACAAAAAATAAGAATTTATGT
ATGACTGTTTTATTTTGAGAATTATGAAATAGTTACACTGTTTATCAATA
AAATTTAATTTGTGA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **cellular component**
Term	Definition
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0005576	extracellular region

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000099	neuron

Vocabulary: **INTERPRO**
Term	Definition
IPR036880	Kunitz_BPTI_sf
IPR002223	Kunitz_BPTI
IPR020901	Prtase_inh_Kunz-CS

Vocabulary: **molecular function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004857	enzyme inhibitor activity
GO:0030414	peptidase inhibitor activity

Vocabulary: **biological process**
Term	Definition
GO:0010466	negative regulation of peptidase activity
GO:0010951	negative regulation of endopeptidase activity
GO:0043086	negative regulation of catalytic activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PRINTS	PR00759	BASICPTASE	coord: 48..58 score: 55.25 coord: 20..34 score: 35.56 coord: 58..73 score: 33.64
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	SMART	SM00131	KU_2	coord: 21..74 e-value: 7.1E-16 score: 68.7
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PFAM	PF00014	Kunitz_BPTI	coord: 23..73 e-value: 8.2E-16 score: 58.0
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PROSITE	PS50279	BPTI_KUNITZ_2	coord: 23..73 score: 14.232
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	CDD	cd00109	KU	coord: 23..73 e-value: 6.20622E-19 score: 70.3566
IPR036880	Pancreatic trypsin inhibitor Kunitz domain superfamily	GENE3D	G3DSA:4.10.410.10		coord: 20..75 e-value: 1.1E-19 score: 71.9
IPR036880	Pancreatic trypsin inhibitor Kunitz domain superfamily	SUPERFAMILY	SSF57362	BPTI-like	coord: 19..74
None	No IPR available	PANTHER	PTHR10083	KUNITZ-TYPE PROTEASE INHIBITOR-RELATED	coord: 21..74
None	No IPR available	PANTHER	PTHR10083:SF328	TISSUE FACTOR PATHWAY INHIBITOR	coord: 21..74
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..18 score: 0.916
IPR020901	Proteinase inhibitor I2, Kunitz, conserved site	PROSITE	PS00280	BPTI_KUNITZ_1	coord: 51..69

Main menu

Kunitz domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

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