Ribosome biogenesis protein NSA2

Overview
NameRibosome biogenesis protein NSA2
Smed IDSMED30014496
Length (bp)833
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ribosome biogenesis protein NSA2 (SMED30014496) t-SNE clustered cells

Violin plots show distribution of expression levels for Ribosome biogenesis protein NSA2 (SMED30014496) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ribosome biogenesis protein NSA2 (SMED30014496) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ribosome biogenesis protein NSA2 (SMED30014496) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014496

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014496h1SMcG0018285 dd_Smed_v4_1012_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Human
Match: NSA2 (NSA2 ribosome biogenesis factor [Source:HGNC Symbol;Acc:HGNC:30728])

HSP 1 Score: 299.286 bits (765), Expect = 7.642e-102
Identity = 160/256 (62.50%), Postives = 207/256 (80.86%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE+R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKESREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Human
Match: NSA2 (NSA2 ribosome biogenesis factor [Source:HGNC Symbol;Acc:HGNC:30728])

HSP 1 Score: 210.305 bits (534), Expect = 5.815e-68
Identity = 104/174 (59.77%), Postives = 138/174 (79.31%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPM 540
            MPQNE+IE H+K++GYRLDY E+KRKKE+R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKESREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPM 174
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Human
Match: NSA2 (NSA2 ribosome biogenesis factor [Source:HGNC Symbol;Acc:HGNC:30728])

HSP 1 Score: 105.531 bits (262), Expect = 3.389e-28
Identity = 51/82 (62.20%), Postives = 63/82 (76.83%), Query Frame = 1 
Query:  295 RKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPM 540
            R+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM
Sbjct:    2 REGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPM 83
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Celegans
Match: W09C5.1 (pep chromosome:WBcel235:I:13626522:13628447:-1 gene:WBGene00012351.1 transcript:W09C5.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:W09C5.1)

HSP 1 Score: 282.337 bits (721), Expect = 1.137e-95
Identity = 152/255 (59.61%), Postives = 201/255 (78.82%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETMS-GDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+K+HG RLD+ ER+RKK AR+ H+ +  A+ L G KAK+Y+K+R+ EKV+++K LK +EEK  K  VE    G +P YLLDR++Q +  +LS  +KQKR +KAGK+ VPIP+VR VS+ EAF + ++GKT RK WKR++TK+TFV   FTRK  K+ERFI+PM LR K+A+VT  +L  TF+LPI+GVKKNP+S +YT LG+ITKGTI+EV+VSE+GMVT GGK+V+G +AQVTN PEN+GC NA
Sbjct:    1 MPQNEHIELHRKRHGRRLDHEERQRKKLARAAHDRSQMAKTLRGHKAKLYHKKRYSEKVEMRKLLKQHEEKDQKNTVEQPDKGAVPAYLLDRQQQTSGTVLSNMIKQKRKQKAGKFNVPIPQVRAVSDAEAFKVVKTGKTNRKGWKRMVTKVTFVGESFTRKPAKFERFIRPMGLRFKKAHVTHPELQTTFHLPIVGVKKNPSSQMYTSLGVITKGTILEVNVSELGMVTQGGKVVWGKFAQVTNNPENDGCINA 255
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Fly
Match: Ip259 (gene:FBgn0025366 transcript:FBtr0080017)

HSP 1 Score: 278.485 bits (711), Expect = 5.006e-94
Identity = 160/255 (62.75%), Postives = 205/255 (80.39%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETM-SGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE++ERH+K +G RLDY ERKRKKEAR   + A KARKL GIKAK++NKER  EK+Q+KK ++ +EEKK KK  E +  G +P YLLDR  Q +A++LS  +KQKR EKAGKW+VPIPKVR  S+ E F + ++GKTKRKAWKR++TK+TFV   FTRK PK+ERFI+PM LR+K+A+VT  +L  TFNLPI+GVKKNP+S ++T LG+ITKGT+IEV++SE+G+VT  GK+V+G YAQVTN PEN+G  NA
Sbjct:    1 MPQNEYMERHRKLYGRRLDYEERKRKKEARLPKDRARKARKLRGIKAKLFNKERRNEKIQIKKKIQAHEEKKVKKQEEKVEDGALPHYLLDRGIQSSAKVLSNMIKQKRKEKAGKWDVPIPKVRAQSDAEVFKVLKTGKTKRKAWKRMVTKVTFVGENFTRKPPKFERFIRPMGLRMKKAHVTHPELKATFNLPIIGVKKNPSSPMFTSLGVITKGTVIEVNISELGLVTQTGKVVWGKYAQVTNNPENDGVINA 255
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Fly
Match: Ip259 (gene:FBgn0025366 transcript:FBtr0331641)

HSP 1 Score: 278.485 bits (711), Expect = 5.006e-94
Identity = 160/255 (62.75%), Postives = 205/255 (80.39%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETM-SGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE++ERH+K +G RLDY ERKRKKEAR   + A KARKL GIKAK++NKER  EK+Q+KK ++ +EEKK KK  E +  G +P YLLDR  Q +A++LS  +KQKR EKAGKW+VPIPKVR  S+ E F + ++GKTKRKAWKR++TK+TFV   FTRK PK+ERFI+PM LR+K+A+VT  +L  TFNLPI+GVKKNP+S ++T LG+ITKGT+IEV++SE+G+VT  GK+V+G YAQVTN PEN+G  NA
Sbjct:    1 MPQNEYMERHRKLYGRRLDYEERKRKKEARLPKDRARKARKLRGIKAKLFNKERRNEKIQIKKKIQAHEEKKVKKQEEKVEDGALPHYLLDRGIQSSAKVLSNMIKQKRKEKAGKWDVPIPKVRAQSDAEVFKVLKTGKTKRKAWKRMVTKVTFVGENFTRKPPKFERFIRPMGLRMKKAHVTHPELKATFNLPIIGVKKNPSSPMFTSLGVITKGTVIEVNISELGLVTQTGKVVWGKYAQVTNNPENDGVINA 255
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Zebrafish
Match: nsa2 (NSA2 ribosome biogenesis homolog (S. cerevisiae) [Source:ZFIN;Acc:ZDB-GENE-030131-488])

HSP 1 Score: 304.294 bits (778), Expect = 4.892e-104
Identity = 160/256 (62.50%), Postives = 208/256 (81.25%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+K+HGYRLD+ E+KRKKE+R  HE + KARK+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++K+K+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK ++KAWKR++TK+ FV  GFTRK PKYERFI+PM LR  +A+VT  +L  TF LPILGVKKNP+S+LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEHIELHRKRHGYRLDHHEKKRKKESREAHERSHKARKMIGLKAKLYHKQRHAEKIQMKKTIKMHEQRKSKQKDDDKTPEGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRQKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFNKAHVTHPELKATFCLPILGVKKNPSSSLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Xenopus
Match: nsa2 (NSA2, ribosome biogenesis homolog [Source:NCBI gene;Acc:550080])

HSP 1 Score: 299.671 bits (766), Expect = 4.778e-102
Identity = 161/256 (62.89%), Postives = 207/256 (80.86%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQN++IE H+K++GYRLDY E+KRKKE+R  HE + KA+KL G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+V+G YAQVTN PEN+GC NA
Sbjct:    1 MPQNDYIELHRKRYGYRLDYHEKKRKKESREAHERSQKAKKLIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPEGAVPAYLLDREGQTRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVVWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Xenopus
Match: nsa2 (NSA2, ribosome biogenesis homolog [Source:NCBI gene;Acc:550080])

HSP 1 Score: 298.516 bits (763), Expect = 5.670e-101
Identity = 160/258 (62.02%), Postives = 207/258 (80.23%), Query Frame = 1 
Query:   19 FSMPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            +  PQN++IE H+K++GYRLDY E+KRKKE+R  HE + KA+KL G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+V+G YAQVTN PEN+GC NA
Sbjct:   39 YKQPQNDYIELHRKRYGYRLDYHEKKRKKESREAHERSQKAKKLIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPEGAVPAYLLDREGQTRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVVWGKYAQVTNNPENDGCINA 296
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Xenopus
Match: nsa2 (NSA2, ribosome biogenesis homolog [Source:NCBI gene;Acc:550080])

HSP 1 Score: 209.92 bits (533), Expect = 9.805e-68
Identity = 105/189 (55.56%), Postives = 144/189 (76.19%), Query Frame = 1 
Query:   28 PQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGT 588
            PQN++IE H+K++GYRLDY E+KRKKE+R  HE + KA+KL G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM   + ++ +  K +G 
Sbjct:    1 PQNDYIELHRKRYGYRLDYHEKKRKKESREAHERSQKAKKLIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPEGAVPAYLLDREGQTRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPME-NMHKSQIIQKMMGV 188
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Mouse
Match: Nsa2 (NSA2 ribosome biogenesis homolog [Source:MGI Symbol;Acc:MGI:1913883])

HSP 1 Score: 298.901 bits (764), Expect = 6.599e-102
Identity = 160/256 (62.50%), Postives = 206/256 (80.47%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKEGREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKDDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Mouse
Match: Nsa2 (NSA2 ribosome biogenesis homolog [Source:MGI Symbol;Acc:MGI:1913883])

HSP 1 Score: 256.914 bits (655), Expect = 8.296e-86
Identity = 143/255 (56.08%), Postives = 186/255 (72.94%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM                          NP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKEGREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKDDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPM--------------------------NPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 229
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. UniProt/SwissProt
Match: sp|O95478|NSA2_HUMAN (Ribosome biogenesis protein NSA2 homolog OS=Homo sapiens OX=9606 GN=NSA2 PE=1 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 3.670e-101
Identity = 160/256 (62.50%), Postives = 207/256 (80.86%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE+R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKESREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. UniProt/SwissProt
Match: sp|Q9CR47|NSA2_MOUSE (Ribosome biogenesis protein NSA2 homolog OS=Mus musculus OX=10090 GN=Nsa2 PE=2 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 4.616e-101
Identity = 160/256 (62.50%), Postives = 206/256 (80.47%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKEGREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKDDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. UniProt/SwissProt
Match: sp|Q3SX11|NSA2_BOVIN (Ribosome biogenesis protein NSA2 homolog OS=Bos taurus OX=9913 GN=NSA2 PE=2 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 4.983e-101
Identity = 160/256 (62.50%), Postives = 206/256 (80.47%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK K+KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKEGREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. UniProt/SwissProt
Match: sp|Q9QYU7|NSA2_RAT (Ribosome biogenesis protein NSA2 homolog OS=Rattus norvegicus OX=10116 GN=Nsa2 PE=2 SV=2)

HSP 1 Score: 293.893 bits (751), Expect = 4.946e-99
Identity = 157/256 (61.33%), Postives = 205/256 (80.08%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE H+K++GYRLDY E+KRKKE R  HE + KA+K+ G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWE P+PKVR   E E   + R+GK K+KAW+R++TK+ FV  GFTRK PKYE+FI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEYIELHRKRYGYRLDYHEKKRKKEGREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKDDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWESPLPKVRAQGETELLKVIRTGKRKKKAWRRMVTKVCFVGDGFTRKPPKYEKFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. UniProt/SwissProt
Match: sp|Q9UU79|NSA2_SCHPO (Ribosome biogenesis protein nsa2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nsa2 PE=1 SV=1)

HSP 1 Score: 276.174 bits (705), Expect = 4.124e-92
Identity = 149/257 (57.98%), Postives = 200/257 (77.82%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK---DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLG-TFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNE+IE   +KHG R D+ ERKRKK AR  H+ +L A+K  GIKAK+Y ++R KEK+Q+KKT+K +EE+   +   D +T  G +P YLLDR+++  A++LS+ +KQKR EKA K+ VP+P+VR V+E+E F + R+GK+K+ +WKR+ITK TFV  GFTR+  KYERFI+PM+LR K+ANVT K+LG T  LPI+GVKKNP S  YT LG++TKGT+IEV+VSE+G+VT GGK+V+G YAQ+TN PE +GC NA
Sbjct:    1 MPQNEYIEESIRKHGRRFDHEERKRKKAAREAHDASLYAQKTRGIKAKLYQEKRRKEKIQMKKTIKQHEERNATQRGSDAQT-QGAVPTYLLDREQESQAKMLSSAVKQKRKEKAAKYSVPLPQVRGVAEEEMFKVIRTGKSKKNSWKRMITKATFVGDGFTRRPVKYERFIRPMALRQKKANVTHKELGVTMQLPIIGVKKNPQSPTYTQLGVLTKGTVIEVNVSELGLVTSGGKVVWGKYAQITNNPELDGCVNA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. TrEMBL
Match: A0A2L1IV00 (TGF beta inducible nuclear protein OS=Dugesia japonica OX=6161 GN=TINP1 PE=2 SV=1)

HSP 1 Score: 463.766 bits (1192), Expect = 8.407e-164
Identity = 243/253 (96.05%), Postives = 250/253 (98.81%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENA KARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVET+ GD+PIYLL+RKKQENAELLSTT+KQKRLEKAGKWEVPIPKV+QVSEKEAF +QRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPM+LRLKRANVTSKKLGTFNLPILGVKKNPNSTLYTGLGIITKGTIIEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA
Sbjct:    1 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENAEKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETVGGDIPIYLLNRKKQENAELLSTTIKQKRLEKAGKWEVPIPKVKQVSEKEAFRVQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMALRLKRANVTSKKLGTFNLPILGVKKNPNSTLYTGLGIITKGTIIEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 253
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. TrEMBL
Match: C1L532 (TGF-beta-inducible nuclear protein 1 OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 331.257 bits (848), Expect = 2.779e-111
Identity = 175/256 (68.36%), Postives = 216/256 (84.38%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETM--SGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+++HGYR DYFERKRKKEAR  HE + KA+KL G+KAKI NKERFKEKVQ+KKTL+M+E K+  ++VE +  SG++P YLLDR +Q +A++LS T+KQKR EKAGKWEVP+PKVR VSE EAF + R+GK+KRKAWKR++TKM FV   FTRK PK+ERFI+PM LR+K+ANVT  +L  TF LPI+GVKKNP+S +YT LG+ITKGTIIEV+VS++G+VTP GK+V+G YAQVTN PENEGC NA
Sbjct:    1 MPQNEHIELHRRRHGYRFDYFERKRKKEAREPHERSRKAKKLRGLKAKIANKERFKEKVQMKKTLRMHELKRKNQNVENVGRSGEVPAYLLDRGEQISAKILSNTVKQKRAEKAGKWEVPLPKVRSVSEAEAFKVVRTGKSKRKAWKRLVTKMCFVGDTFTRKPPKFERFIRPMGLRVKKANVTHPELKTTFQLPIIGVKKNPSSPMYTTLGVITKGTIIEVNVSDLGLVTPSGKVVWGKYAQVTNNPENEGCVNA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. TrEMBL
Match: A0A5J4NDA3 (Ribosome biogenesis protein NSA2 OS=Paragonimus westermani OX=34504 GN=DEA37_0013970 PE=4 SV=1)

HSP 1 Score: 329.717 bits (844), Expect = 9.052e-111
Identity = 173/256 (67.58%), Postives = 215/256 (83.98%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETM--SGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+K+HG+R DYFERKRKKEAR  HE + KA+KL GI+AK+ N+ERFKEKVQ+KKTL+M+E KKTK+ +     SG+ P YLLDR +Q +A++LS T+KQKR EKAGKWEVP+PKVR V+E EAF I R+GK+KRKAWKR++TKM FV   FTRK PK+ERFI+PM LR+++ANVT  +L  TF LPI+GVKKNP+S +YT LG+ITKGT+IEV+VS++G+VTPGGK+V+G YAQVTN PENEGC NA
Sbjct:    1 MPQNEHIELHRKRHGFRYDYFERKRKKEAREPHERSAKAKKLRGIRAKLANRERFKEKVQMKKTLRMHELKKTKQKIGDAGGSGEKPAYLLDRDEQVSAKVLSNTVKQKRAEKAGKWEVPLPKVRSVAESEAFKIVRTGKSKRKAWKRMVTKMCFVGDTFTRKPPKFERFIRPMGLRVRKANVTHPELKATFQLPIIGVKKNPSSPMYTSLGVITKGTVIEVNVSDLGLVTPGGKVVWGKYAQVTNNPENEGCVNA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. TrEMBL
Match: C1LGQ9 (TGF-beta-inducible nuclear protein 1 OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 327.791 bits (839), Expect = 5.380e-110
Identity = 174/256 (67.97%), Postives = 215/256 (83.98%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETM--SGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+++HGYR DYFERKRKKEAR  HE + KA+KL G+KAKI NK+RFKEKVQ+KKTL+M+E K   ++VE +  SG++P YLLDR +Q +A++LS T+KQKR EKAGKWEVP+PKVR VSE EAF + R+GK+KRKAWKR++TKM FV   FTRK PK+ERFI+PM LR+K+ANVT  +L  TF LPI+GVKKNP+S +YT LG+ITKGTIIEV+VS++G+VTP GK+V+G YAQVTN PENEGC NA
Sbjct:    1 MPQNEHIELHRRRHGYRFDYFERKRKKEAREPHERSRKAKKLRGLKAKIANKKRFKEKVQMKKTLRMHELKGKNQNVENVGRSGEVPAYLLDRGEQISAKILSNTVKQKRAEKAGKWEVPLPKVRSVSEAEAFKVVRTGKSKRKAWKRLVTKMCFVGDTFTRKPPKFERFIRPMGLRVKKANVTHPELKTTFQLPIIGVKKNPSSPMYTTLGVITKGTIIEVNVSDLGLVTPSGKVVWGKYAQVTNNPENEGCVNA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. TrEMBL
Match: A0A0R3SYY8 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS10981 PE=4 SV=1)

HSP 1 Score: 325.865 bits (834), Expect = 3.893e-109
Identity = 175/256 (68.36%), Postives = 213/256 (83.20%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETM--SGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+K+HGYRLDYFERKRK+EAR  HE A KARKL G+KAKI NKERFKEKVQ+KKT++M+E +  K++      SG+ P++LLDR++Q  +++LS T+KQKR EKAGKW+VPIPKV+ VSE +AF I +SGK+KRKAWKR++TKM FV   FTRK PK+ERFI+PM+LR KRANV   +   TF LPI+GVKKNP+S LYT LGIITKGTIIEV+VSE+G+VTPGGK+V+G YAQVTN PENEGC NA
Sbjct:    1 MPQNEHIELHRKRHGYRLDYFERKRKREAREPHERAQKARKLRGLKAKIANKERFKEKVQMKKTIRMHEMRNVKQNAGEAGGSGEKPVFLLDREEQNRSKILSNTIKQKRAEKAGKWQVPIPKVQTVSEADAFRIVKSGKSKRKAWKRLVTKMCFVGDNFTRKPPKFERFIRPMALRAKRANVVHPETRDTFCLPIIGVKKNPSSPLYTSLGIITKGTIIEVNVSEMGLVTPGGKVVWGKYAQVTNHPENEGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Cavefish
Match: nsa2 (NSA2 ribosome biogenesis factor [Source:NCBI gene;Acc:103026946])

HSP 1 Score: 305.449 bits (781), Expect = 1.331e-104
Identity = 162/256 (63.28%), Postives = 207/256 (80.86%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+K+HGYRLDY E+KRKKE+R  HE + KA+KL G+KAK+Y+K+R  EK+Q+KKT+KM+E++ TK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + R+GK ++KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEHIELHRKRHGYRLDYHEKKRKKESREVHERSHKAKKLIGLKAKLYHKQRHAEKIQMKKTIKMHEKRSTKQKDDEKTPEGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRQKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Yeast
Match: NSA2 (Protein constituent of 66S pre-ribosomal particles; contributes to processing of the 27S pre-rRNA; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2 [Source:SGD;Acc:S000000928])

HSP 1 Score: 261.536 bits (667), Expect = 4.019e-88
Identity = 145/257 (56.42%), Postives = 197/257 (76.65%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTK---KDVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLG-TFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQN++IERH K+HG RLD+ ERKRK+EAR  H+ + +A+KLTG K K + K+R+ EKV ++K +K +E+ K K   K ++T    +P YLLDR++   A+ +S+++KQKRLEKA K+ VP+PKVR +SE+E F + ++GK++ K+WKR+ITK TFV  GFTR+  K ER I+P +LR K+ANVT  +LG T  LPIL VKKNP S +YT LG++TKGTIIEV+VSE+GMVT GGK+V+G YAQVTN P+ +GC NA
Sbjct:    1 MPQNDYIERHIKQHGKRLDHEERKRKREARESHKISERAQKLTGWKGKQFAKKRYAEKVSMRKKIKAHEQSKVKGSSKPLDTDGDALPTYLLDREQNNTAKAISSSIKQKRLEKADKFSVPLPKVRGISEEEMFKVIKTGKSRSKSWKRMITKHTFVGEGFTRRPVKMERIIRPSALRQKKANVTHPELGVTVFLPILAVKKNPQSPMYTQLGVLTKGTIIEVNVSELGMVTAGGKVVWGKYAQVTNEPDRDGCVNA 257
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Nematostella
Match: EDO34448 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPC1])

HSP 1 Score: 308.145 bits (788), Expect = 3.895e-106
Identity = 164/256 (64.06%), Postives = 207/256 (80.86%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTK--KDVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE+ +K+HGYRLD+FERKRKKE R  HE A +A+KL G+KAK+YNK+R  EKVQ+KKT+K++EEKKTK   + +   G +P YLLDR+ Q  A++LS  +KQKR EKAGKW+VP+PKVR V E E F + ++GKT+RK WKR++TK  FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPI+GVKKNP S+LYT LG+ITKGT++EV+VSE+G+VT GGK+V+G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEHIEQSRKRHGYRLDHFERKRKKEGRQPHEQAERAKKLHGLKAKLYNKKRHAEKVQMKKTMKLHEEKKTKARNNEKVPEGAVPAYLLDREGQSRAKVLSNMVKQKRKEKAGKWDVPLPKVRAVGEDEVFKVIKTGKTRRKGWKRMVTKACFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPIIGVKKNPQSSLYTQLGVITKGTVVEVNVSELGLVTQGGKVVWGKYAQVTNNPENDGCINA 256
[Close]
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Medaka
Match: nsa2 (NSA2 ribosome biogenesis factor [Source:NCBI gene;Acc:101158868])

HSP 1 Score: 305.834 bits (782), Expect = 1.018e-104
Identity = 162/256 (63.28%), Postives = 208/256 (81.25%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKK--DVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKL-GTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIE H+K+HGYRLD+ ERKRKKE+R  HE + KA+KL G+KAK+Y+K+R  EK+Q+KKT+KM+E++KTK+  D +T  G +P YLLDR+ Q  A++LS  +KQKR EKAGKWEVP+PKVR   E E   + ++GK ++KAWKR++TK+ FV  GFTRK PKYERFI+PM LR K+A+VT  +L  TF LPILGVKKNP+S LYT LG+ITKGT+IEV+VSE+G+VT GGK+++G YAQVTN PEN+GC NA
Sbjct:    1 MPQNEHIELHRKRHGYRLDHHERKRKKESREAHERSHKAKKLIGLKAKLYHKQRHAEKIQMKKTIKMHEQRKTKQKNDDKTPEGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIKTGKRQKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTSLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINA 256
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BLAST of Ribosome biogenesis protein NSA2 vs. Planmine SMEST
Match: SMESG000028599.1 (SMESG000028599.1)

HSP 1 Score: 483.026 bits (1242), Expect = 6.770e-175
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 1 
Query:   25 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGTFNLPILGVKKNPNSXXXXXXXXXXXXXXXEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 783
            MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGTFNLPILGVKKNPNSTLYTGLGIITKGTIIEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA
Sbjct:    1 MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIYNKERFKEKVQLKKTLKMNEEKKTKKDVETMSGDMPIYLLDRKKQENAELLSTTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITKMTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGTFNLPILGVKKNPNSTLYTGLGIITKGTIIEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGCCNA 253
The following BLAST results are available for this feature:
BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
NSA27.642e-10262.50NSA2 ribosome biogenesis factor [Source:HGNC Symbo... [more]
NSA25.815e-6859.77NSA2 ribosome biogenesis factor [Source:HGNC Symbo... [more]
NSA23.389e-2862.20NSA2 ribosome biogenesis factor [Source:HGNC Symbo... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
W09C5.11.137e-9559.61pep chromosome:WBcel235:I:13626522:13628447:-1 gen... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ip2595.006e-9462.75gene:FBgn0025366 transcript:FBtr0080017[more]
Ip2595.006e-9462.75gene:FBgn0025366 transcript:FBtr0331641[more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nsa24.892e-10462.50NSA2 ribosome biogenesis homolog (S. cerevisiae) [... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
nsa24.778e-10262.89NSA2, ribosome biogenesis homolog [Source:NCBI gen... [more]
nsa25.670e-10162.02NSA2, ribosome biogenesis homolog [Source:NCBI gen... [more]
nsa29.805e-6855.56NSA2, ribosome biogenesis homolog [Source:NCBI gen... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Nsa26.599e-10262.50NSA2 ribosome biogenesis homolog [Source:MGI Symbo... [more]
Nsa28.296e-8656.08NSA2 ribosome biogenesis homolog [Source:MGI Symbo... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O95478|NSA2_HUMAN3.670e-10162.50Ribosome biogenesis protein NSA2 homolog OS=Homo s... [more]
sp|Q9CR47|NSA2_MOUSE4.616e-10162.50Ribosome biogenesis protein NSA2 homolog OS=Mus mu... [more]
sp|Q3SX11|NSA2_BOVIN4.983e-10162.50Ribosome biogenesis protein NSA2 homolog OS=Bos ta... [more]
sp|Q9QYU7|NSA2_RAT4.946e-9961.33Ribosome biogenesis protein NSA2 homolog OS=Rattus... [more]
sp|Q9UU79|NSA2_SCHPO4.124e-9257.98Ribosome biogenesis protein nsa2 OS=Schizosaccharo... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2L1IV008.407e-16496.05TGF beta inducible nuclear protein OS=Dugesia japo... [more]
C1L5322.779e-11168.36TGF-beta-inducible nuclear protein 1 OS=Schistosom... [more]
A0A5J4NDA39.052e-11167.58Ribosome biogenesis protein NSA2 OS=Paragonimus we... [more]
C1LGQ95.380e-11067.97TGF-beta-inducible nuclear protein 1 OS=Schistosom... [more]
A0A0R3SYY83.893e-10968.36Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nsa21.331e-10463.28NSA2 ribosome biogenesis factor [Source:NCBI gene;... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
NSA24.019e-8856.42Protein constituent of 66S pre-ribosomal particles... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO344483.895e-10664.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nsa21.018e-10463.28NSA2 ribosome biogenesis factor [Source:NCBI gene;... [more]
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BLAST of Ribosome biogenesis protein NSA2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000028599.16.770e-175100.00SMESG000028599.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014496 ID=SMED30014496|Name=Ribosome biogenesis protein NSA2|organism=Schmidtea mediterranea sexual|type=transcript|length=833bp
ATTTTTTGAACTCTAAATTTCAGCATGCCACAAAACGAACACATTGAAAG
GCATCAGAAAAAACATGGTTATCGCTTAGACTATTTTGAACGAAAGCGAA
AGAAAGAAGCACGTTCATACCATGAAAATGCTCTTAAGGCCAGAAAATTA
ACTGGTATCAAAGCCAAGATTTATAATAAAGAAAGATTTAAGGAAAAAGT
CCAATTAAAGAAAACCTTAAAAATGAATGAGGAAAAGAAAACTAAGAAAG
ATGTTGAGACTATGAGTGGGGATATGCCAATTTATTTATTAGACAGAAAA
AAGCAAGAAAATGCTGAACTTCTCAGTACAACAATGAAACAAAAACGCTT
AGAAAAGGCTGGTAAGTGGGAGGTGCCTATTCCAAAAGTTCGTCAAGTTA
GTGAGAAAGAAGCTTTCACAATTCAACGTAGTGGTAAAACTAAACGCAAA
GCGTGGAAGAGAGTTATTACAAAAATGACATTTGTTGATCCTGGATTCAC
TAGAAAGGCCCCAAAATATGAAAGATTCATCAAACCCATGTCTTTAAGAT
TAAAACGAGCCAACGTTACGAGCAAAAAATTAGGTACTTTCAATTTACCA
ATTCTCGGTGTGAAAAAGAATCCAAATTCAACTTTATACACGGGCTTAGG
AATAATTACCAAAGGAACAATTATCGAGGTTGATGTATCAGAAATAGGAA
TGGTTACACCAGGTGGTAAAATTGTGTATGGTAGCTACGCACAAGTTACA
AATGTTCCTGAGAACGAAGGCTGTTGCAACGCTATTTTGTTGGAATAAAT
CATTTGTATATTGGAAAAAAAAGATCGGAAGAG
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protein sequence of SMED30014496-orf-1

>SMED30014496-orf-1 ID=SMED30014496-orf-1|Name=SMED30014496-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=258bp
MPQNEHIERHQKKHGYRLDYFERKRKKEARSYHENALKARKLTGIKAKIY
NKERFKEKVQLKKTLKMNEEKKTKKDVETMSGDMPIYLLDRKKQENAELL
STTMKQKRLEKAGKWEVPIPKVRQVSEKEAFTIQRSGKTKRKAWKRVITK
MTFVDPGFTRKAPKYERFIKPMSLRLKRANVTSKKLGTFNLPILGVKKNP
NSTLYTGLGIITKGTIIEVDVSEIGMVTPGGKIVYGSYAQVTNVPENEGC
CNAILLE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000463maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000466maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006364rRNA processing
GO:0042254ribosome biogenesis
GO:0042273ribosomal large subunit biogenesis
GO:0000460maturation of 5.8S rRNA
GO:0000470maturation of LSU-rRNA
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005730nucleolus
GO:0030687preribosome, large subunit precursor
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR039411NSA2_fam
IPR022309Ribosomal_S8e/biogenesis_NSA2
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.40.10.310coord: 177..257
e-value: 5.1E-8
score: 34.4
IPR022309Ribosomal protein S8e/ribosomal biogenesis NSA2PFAMPF01201Ribosomal_S8ecoord: 33..256
e-value: 6.8E-36
score: 123.5
IPR039411Ribosomal biogenesis NSA2 familyPANTHERPTHR12642FAMILY NOT NAMEDcoord: 1..256