DUF3421 domain-containing protein

Overview
NameDUF3421 domain-containing protein
Smed IDSMED30014482
Length (bp)675
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DUF3421 domain-containing protein (SMED30014482) t-SNE clustered cells

Violin plots show distribution of expression levels for DUF3421 domain-containing protein (SMED30014482) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DUF3421 domain-containing protein (SMED30014482) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DUF3421 domain-containing protein (SMED30014482) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014482

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30014482h1SMcG0009595 dd_Smed_v4_2232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014482h1SMcG0009595 dd_Smed_v4_2232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30014482h1SMcG0009595 dd_Smed_v4_2232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014482h1SMcG0009595 dd_Smed_v4_2232_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014482h1SMcG0009595 dd_Smed_v6_2232_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0345371)

HSP 1 Score: 102.064 bits (253), Expect = 7.778e-26
Identity = 58/152 (38.16%), Postives = 85/152 (55.92%), Query Frame = 3 
Query:  180 IMSGNLSWKGAKNGDIPSNSVQVG-----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTA-GNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            ++  + SW  +  G +P +++QVG     E  YV R   +G +TPGK+   H+  YI YGG+E  +E YEVL +     P++    WI +S + +  G ++GG  A G+ +YV RA  EGD+   K  P   CAY PY G EV K D+E+L 
Sbjct:   71 LVGDHFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQ-----PET----WIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213

HSP 2 Score: 87.4261 bits (215), Expect = 2.444e-20
Identity = 50/150 (33.33%), Postives = 78/150 (52.00%), Query Frame = 3 
Query:  189 GNLSWKGAK-NGDIPSNSVQVG-----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGAL-IGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            G+ +W      G +P  ++  G     +  +V RA H GEM P K+  G +  Y+ +GG+EI+  ++EVL  +         + WI +S  +VP  A+ +G +  G PLYV R   +G +  GK +P+ QC Y PY G+E     +EVL 
Sbjct:    2 GDYTWISTNVYGSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLVGD--------HFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143

HSP 3 Score: 84.7297 bits (208), Expect = 2.641e-19
Identity = 45/124 (36.29%), Postives = 66/124 (53.23%), Query Frame = 3 
Query:  249 GEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTA-GNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            G+  YV RA H+G++ P K+       Y+ YGG E+   +YE+L   G        Y W+  S  NVP  A++ G T+ G PL++ RA   G +  GK + +  C Y P+DG+EV    +EVL 
Sbjct:  169 GDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLAGYG--------YGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284

HSP 4 Score: 57.3806 bits (137), Expect = 1.231e-9
Identity = 26/67 (38.81%), Postives = 39/67 (58.21%), Query Frame = 3 
Query:  201 WKGAKNGDIPSNSVQVG-----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTE 386
            W    +G++P N+V  G     E  ++ RA H G +TPGKI + H   YI + GEE+ ++ YEVL +
Sbjct:  219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLVK 285
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0087792)

HSP 1 Score: 102.064 bits (253), Expect = 7.778e-26
Identity = 58/152 (38.16%), Postives = 85/152 (55.92%), Query Frame = 3 
Query:  180 IMSGNLSWKGAKNGDIPSNSVQVG-----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTA-GNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            ++  + SW  +  G +P +++QVG     E  YV R   +G +TPGK+   H+  YI YGG+E  +E YEVL +     P++    WI +S + +  G ++GG  A G+ +YV RA  EGD+   K  P   CAY PY G EV K D+E+L 
Sbjct:   71 LVGDHFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQ-----PET----WIASSGRGIVPGTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213

HSP 2 Score: 87.4261 bits (215), Expect = 2.444e-20
Identity = 50/150 (33.33%), Postives = 78/150 (52.00%), Query Frame = 3 
Query:  189 GNLSWKGAK-NGDIPSNSVQVG-----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGAL-IGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            G+ +W      G +P  ++  G     +  +V RA H GEM P K+  G +  Y+ +GG+EI+  ++EVL  +         + WI +S  +VP  A+ +G +  G PLYV R   +G +  GK +P+ QC Y PY G+E     +EVL 
Sbjct:    2 GDYTWISTNVYGSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLVGD--------HFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143

HSP 3 Score: 84.7297 bits (208), Expect = 2.641e-19
Identity = 45/124 (36.29%), Postives = 66/124 (53.23%), Query Frame = 3 
Query:  249 GEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTA-GNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            G+  YV RA H+G++ P K+       Y+ YGG E+   +YE+L   G        Y W+  S  NVP  A++ G T+ G PL++ RA   G +  GK + +  C Y P+DG+EV    +EVL 
Sbjct:  169 GDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLAGYG--------YGWVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284

HSP 4 Score: 57.3806 bits (137), Expect = 1.231e-9
Identity = 26/67 (38.81%), Postives = 39/67 (58.21%), Query Frame = 3 
Query:  201 WKGAKNGDIPSNSVQVG-----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTE 386
            W    +G++P N+V  G     E  ++ RA H G +TPGKI + H   YI + GEE+ ++ YEVL +
Sbjct:  219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLVK 285
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG10527 (gene:FBgn0034583 transcript:FBtr0071541)

HSP 1 Score: 98.2117 bits (243), Expect = 2.712e-24
Identity = 56/147 (38.10%), Postives = 82/147 (55.78%), Query Frame = 3 
Query:  186 SGNLSWKGAKNGDIPSNSVQVG----EGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTA-GNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEV 611
            SG   W  A NG++P N+++ G    E  Y+ARA H+G++ PGK+   H   Y+++GG E    EYEVL         +G  +W+     N+P  AL  G TA G PL++ RA+ +G I  GK  P+  C Y PY G+E+   +FE+
Sbjct:  154 SGPGCWVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLC--------AGGGQWLPVDAGNIPPNALPAGETAEGEPLFIGRATHDGTITVGKVQPSHGCCYIPYGGEELAYKEFEI 292

HSP 2 Score: 55.8398 bits (133), Expect = 4.536e-9
Identity = 27/71 (38.03%), Postives = 40/71 (56.34%), Query Frame = 3 
Query:  180 IMSGNLSWKGAKNGDIPSNSVQVGEGC-----YVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEV 377
            + +G   W     G+IP N++  GE       ++ RA H G +T GK+   H  CYI YGGEE+A +E+E+
Sbjct:  222 LCAGGGQWLPVDAGNIPPNALPAGETAEGEPLFIGRATHDGTITVGKVQPSHGCCYIPYGGEELAYKEFEI 292

HSP 3 Score: 49.2914 bits (116), Expect = 8.168e-7
Identity = 25/66 (37.88%), Postives = 35/66 (53.03%), Query Frame = 3 
Query:  423 WILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCC 620
            W+  +   VP  AL GG  +   LY+ARA  EGD+  GK +P+    Y  + G E    ++EVLC 
Sbjct:  159 WVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLCA 224
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG44250 (gene:FBgn0265185 transcript:FBtr0339104)

HSP 1 Score: 85.1149 bits (209), Expect = 1.538e-19
Identity = 49/146 (33.56%), Postives = 72/146 (49.32%), Query Frame = 3 
Query:  195 LSWKGAKNGDIPSNSVQ-----VGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGN-PLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVL 614
             +W  + +G +P N+V+      GE  YV R  H G +T GK+   H   YI +GG+E+ +  YEVL ++  D        W+  S    P GA+I G  +   P+Y  RA  EG++   K  P+   AY  + G E  K  +EVL
Sbjct:   75 FAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVLIKQQHDV-------WVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVL 213

HSP 2 Score: 79.337 bits (194), Expect = 2.291e-17
Identity = 44/119 (36.97%), Postives = 61/119 (51.26%), Query Frame = 3 
Query:  261 YVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGST-AGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVL 614
            YV R+ H+GE  P K+       Y++YGG E     YEVL  +G        + W+ +S   VP  A+  G+T  G PLYV R    G +  GK +P+  C Y P+ G+EV    +EVL
Sbjct:   31 YVGRSFHEGENLPAKVVPSKGCAYVAYGGAEHTKTHYEVLVGQG--------FAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVL 141

HSP 3 Score: 71.2478 bits (173), Expect = 1.797e-14
Identity = 39/119 (32.77%), Postives = 54/119 (45.38%), Query Frame = 3 
Query:  261 YVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            Y  RAMH+GEM P K+     + Y+ +GG E     YEVLT  G        Y W+       P     G +  G  LY  R   +G +  G    + +C Y PY G+E+    +E+LC
Sbjct:  174 YAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVLTGYG--------YVWMNCGHSIPPNAVTTGRARNGELLYYGRGHYQGSLTPGLISASQRCLYIPYGGREIRINSYEILC 284
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG44250 (gene:FBgn0265185 transcript:FBtr0339105)

HSP 1 Score: 85.1149 bits (209), Expect = 1.660e-19
Identity = 49/146 (33.56%), Postives = 72/146 (49.32%), Query Frame = 3 
Query:  195 LSWKGAKNGDIPSNSVQ-----VGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAG-NPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVL 614
             +W  + +G +P N+V+      GE  YV R  H G +T GK+   H   YI +GG+E+ +  YEVL ++  D        W+  S    P GA+I G  +   P+Y  RA  EG++   K  P+   AY  + G E  K  +EVL
Sbjct:   86 FAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVLIKQQHDV-------WVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVL 224

HSP 2 Score: 79.337 bits (194), Expect = 2.087e-17
Identity = 44/119 (36.97%), Postives = 61/119 (51.26%), Query Frame = 3 
Query:  261 YVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGST-AGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVL 614
            YV R+ H+GE  P K+       Y++YGG E     YEVL  +G        + W+ +S   VP  A+  G+T  G PLYV R    G +  GK +P+  C Y P+ G+EV    +EVL
Sbjct:   42 YVGRSFHEGENLPAKVVPSKGCAYVAYGGAEHTKTHYEVLVGQG--------FAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVL 152

HSP 3 Score: 71.2478 bits (173), Expect = 1.910e-14
Identity = 39/119 (32.77%), Postives = 54/119 (45.38%), Query Frame = 3 
Query:  261 YVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLC 617
            Y  RAMH+GEM P K+     + Y+ +GG E     YEVLT  G        Y W+       P     G +  G  LY  R   +G +  G    + +C Y PY G+E+    +E+LC
Sbjct:  185 YAGRAMHEGEMLPAKVVPSKGTAYVCFGGYEFQKPSYEVLTGYG--------YVWMNCGHSIPPNAVTTGRARNGELLYYGRGHYQGSLTPGLISASQRCLYIPYGGREIRINSYEILC 295
[Close]
BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q66S17|NATT3_THANI (Natterin-3 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 9.815e-7
Identity = 28/80 (35.00%), Postives = 45/80 (56.25%), Query Frame = 3 
Query:  144 RNCLIFL*LISKIMSGNLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLT 383
            + C  F  L+++    NL WKG  +G +P N+V+V E  YV     K +   GK+   H++ ++ + GEE   ++YEVLT
Sbjct:  107 KTCSGFSILVNRDNFENLEWKGGSDGSVPKNAVEVCEKVYVG----KNKYGLGKVHTKHEALFLPWHGEEHWYKDYEVLT 182
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H8B2 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012514g1 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.986e-34
Identity = 70/141 (49.65%), Postives = 87/141 (61.70%), Query Frame = 3 
Query:  195 LSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKC-DFEVL 614
            LSW  A NGDIP+ +V+ GE CYVARA H+GE  PGK+  GH S Y+ YG EE+   EYEVL          G Y+W+     +VP GAL+ G   G PLYVARA+ EG++C GK  P  +CAY  + GKE     D+EVL
Sbjct:   16 LSWHPASNGDIPAGAVKAGEDCYVARAKHEGEWVPGKLSAGHTSAYVPYGDEEVPYSEYEVLVSSEIGHFGCG-YQWLPAKGNSVPKGALVSGYDNGAPLYVARATFEGEVCCGKAQPDLECAYISWGGKEHKIVDDYEVL 155
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8H2V3 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.215e-32
Identity = 64/145 (44.14%), Postives = 89/145 (61.38%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAG-NPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCK 623
             LSW  A+ G IP+ ++QVG G +VARA H GE+ PGK   GH++ Y+SYGG+E AV +YE+L +          Y W     ++VP GALIGG +A   P Y+ R+ I G++ AGK +    CAYFP+ G+E    +FE+L  K
Sbjct:   22 TLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHQAAYVSYGGQEHAVSDYEILCDTHMQCFGC-NYTWERCHGRSVPRGALIGGYSADRQPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEILVLK 165
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A267EA64 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026761g1 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.486e-32
Identity = 64/145 (44.14%), Postives = 89/145 (61.38%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAG-NPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCK 623
             LSW  A+ G IP+ ++QVG G +VARA H GE+ PGK   GH++ Y+SYGG+E AV +YE+L +          Y W     ++VP GALIGG +A   P Y+ R+ I G++ AGK +    CAYFP+ G+E    +FE+L  K
Sbjct:   20 TLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHQAAYVSYGGQEHAVSDYEILCDTHMQCFGC-NYTWERCHGRSVPRGALIGGYSADRQPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEILVLK 163
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A1I8JBN4 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020417g2 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 2.787e-32
Identity = 64/145 (44.14%), Postives = 89/145 (61.38%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAG-NPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCK 623
             LSW  A+ G IP+ ++QVG G +VARA H GE+ PGK   GH++ Y+SYGG+E AV +YE+L +          Y W     ++VP GALIGG +A   P Y+ R+ I G++ AGK +    CAYFP+ G+E    +FE+L  K
Sbjct:   24 TLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHQAAYVSYGGQEHAVSDYEILCDTHMQCFGC-NYTWERCHGRSVPRGALIGGYSADRQPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEILVLK 167
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A267GMN7 (DUF3421 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig020417g1 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 4.370e-32
Identity = 64/145 (44.14%), Postives = 88/145 (60.69%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGN-PLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCK 623
             LSW  A+ G IP+ ++QVG G +VARA H GE+ PGK   GH + Y+SYGG+E AV +YE+L +          Y W     ++VP GALIGG +A   P Y+ R+ I G++ AGK +    CAYFP+ G+E    +FE+L  K
Sbjct:   22 TLSWIPARGGQIPNGAIQVGHGVFVARAQHAGELIPGKASMGHHAAYVSYGGQEHAVSDYEILCDTHMQCFGC-NYTWERCHGRSVPRGALIGGYSADRKPYYIGRSQINGEMVAGKVFEPHGCAYFPWGGQEHRSNEFEILVLK 165
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BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000051527.1 (SMESG000051527.1)

HSP 1 Score: 301.982 bits (772), Expect = 6.154e-106
Identity = 146/149 (97.99%), Postives = 148/149 (99.33%), Query Frame = 3 
Query:  183 MSGNLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK 629
            MSGNLSWK AKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPD+GTYEWILT+EQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK
Sbjct:    1 MSGNLSWKAAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDAGTYEWILTNEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK 149
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074377.1 (SMESG000074377.1)

HSP 1 Score: 140.584 bits (353), Expect = 4.662e-42
Identity = 66/145 (45.52%), Postives = 90/145 (62.07%), Query Frame = 3 
Query:  195 LSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK 629
            +SW+ A NG+IP  +V+ G  C+V RA   G+  PGK+++GHK  Y+SYGG+EI V  YE L E      D   ++W      NVP GA++GG+  G PLY+AR+ + G+   GKY+P   CAYFP+ G+E  K   EVLCC  K
Sbjct:   16 VSWQKASNGEIPHRTVKAGGKCFVGRAQQNGQYIPGKLYEGHKGVYVSYGGKEICVNNYEALVETNVLHNDG--FQWKYVDGNNVPSGAIVGGTDNGQPLYIARSEVNGEPVVGKYFPPHGCAYFPFGGEEHRKTGVEVLCCVAK 158
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000029913.1 (SMESG000029913.1)

HSP 1 Score: 128.642 bits (322), Expect = 1.944e-37
Identity = 61/146 (41.78%), Postives = 87/146 (59.59%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK 629
            ++SW   ++G+IP  +V  G+ CYVARA H  E  PGK  +GH S Y+SY G+EIA  EY++L +       S   EW  T    +P  +++GG+    PLY+A+++I G++  GKY P   C +FPY GKE  K   E+LCC  K
Sbjct:   15 SVSWIDCQDGNIPDKAVSAGDSCYVARAKHLDEYIPGKFVQGHTSAYVSYDGKEIAKSEYQILVDSSILGGPS--IEWHYTRGNQIPYRSIVGGTDNERPLYIAKSAINGEVVVGKYLPEFACGFFPYGGKEHRKSMVEILCCTTK 158
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074396.1 (SMESG000074396.1)

HSP 1 Score: 128.642 bits (322), Expect = 2.045e-37
Identity = 68/146 (46.58%), Postives = 86/146 (58.90%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK 629
            +LSW  A NG IP  +V+ G   +VARA H G+  PGK  +G+   ++S+GG E A   YE+L E        G YEW  T+ +NVP GAL+GG   G PLYVAR+ IE  +  GKYYP   CAY PY G+E    + EVLC   K
Sbjct:   15 SLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKG-YEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 159
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000074378.1 (SMESG000074378.1)

HSP 1 Score: 128.642 bits (322), Expect = 2.045e-37
Identity = 68/146 (46.58%), Postives = 86/146 (58.90%), Query Frame = 3 
Query:  192 NLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCYISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTAGNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK 629
            +LSW  A NG IP  +V+ G   +VARA H G+  PGK  +G+   ++S+GG E A   YE+L E        G YEW  T+ +NVP GAL+GG   G PLYVAR+ IE  +  GKYYP   CAY PY G+E    + EVLC   K
Sbjct:   15 SLSWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKG-YEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLCFTTK 159
The following BLAST results are available for this feature:
BLAST of DUF3421 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG133217.778e-2638.16gene:FBgn0033787 transcript:FBtr0345371[more]
CG133217.778e-2638.16gene:FBgn0033787 transcript:FBtr0087792[more]
CG105272.712e-2438.10gene:FBgn0034583 transcript:FBtr0071541[more]
CG442501.538e-1933.56gene:FBgn0265185 transcript:FBtr0339104[more]
CG442501.660e-1933.56gene:FBgn0265185 transcript:FBtr0339105[more]
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BLAST of DUF3421 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q66S17|NATT3_THANI9.815e-735.00Natterin-3 OS=Thalassophryne nattereri OX=289382 P... [more]
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BLAST of DUF3421 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8H8B21.986e-3449.65DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A1I8H2V32.215e-3244.14DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A267EA642.486e-3244.14DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A1I8JBN42.787e-3244.14DUF3421 domain-containing protein OS=Macrostomum l... [more]
A0A267GMN74.370e-3244.14DUF3421 domain-containing protein OS=Macrostomum l... [more]
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BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000051527.16.154e-10697.99SMESG000051527.1[more]
SMESG000074377.14.662e-4245.52SMESG000074377.1[more]
SMESG000029913.11.944e-3741.78SMESG000029913.1[more]
SMESG000074396.12.045e-3746.58SMESG000074396.1[more]
SMESG000074378.12.045e-3746.58SMESG000074378.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014482 ID=SMED30014482|Name=DUF3421 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=675bp
CTTTTTCTGACCTCCAAAGCCAGCTTGATTTCATTTTATCTGAAAAAATA
TCAGTTTAATTAACTGATCTGATTTTCCTGTTTTGGTGCGTAAACTGATG
AATATAAATACACAAGGGAAATTTTCAATTTAGTTGTTGCTGTAGAAATT
GTTTAATTTTTCTGTAATTGATATCAAAAATCATGTCAGGCAATCTCTCG
TGGAAAGGGGCCAAAAATGGAGATATTCCTTCAAATTCTGTTCAAGTTGG
TGAAGGTTGTTATGTTGCCAGGGCTATGCATAAAGGCGAGATGACTCCAG
GGAAAATATTTAAGGGTCATAAATCTTGCTATATATCTTATGGAGGAGAA
GAAATAGCAGTTGAGGAATATGAGGTTCTTACAGAAGAGGGTAAAGACGC
TCCTGATTCAGGAACATATGAATGGATTCTTACAAGCGAACAAAACGTTC
CTCTTGGTGCTTTGATCGGTGGTTCTACTGCTGGAAATCCACTTTATGTT
GCAAGAGCATCAATTGAAGGTGACATATGTGCAGGGAAATATTATCCTGC
TGCCCAATGTGCTTATTTTCCATATGACGGAAAAGAAGTTCCAAAGTGTG
ATTTTGAAGTTTTGTGTTGCAAGCCAAAATGAACATTCAAAAATAACAAC
AATTTTTTCTTTTTTTTATTTATAT
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protein sequence of SMED30014482-orf-1

>SMED30014482-orf-1 ID=SMED30014482-orf-1|Name=SMED30014482-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MSGNLSWKGAKNGDIPSNSVQVGEGCYVARAMHKGEMTPGKIFKGHKSCY
ISYGGEEIAVEEYEVLTEEGKDAPDSGTYEWILTSEQNVPLGALIGGSTA
GNPLYVARASIEGDICAGKYYPAAQCAYFPYDGKEVPKCDFEVLCCKPK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR006616DM9_repeat
IPR024518DUF3421
Vocabulary: molecular function
TermDefinition
GO:0008168methyltransferase activity
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0032259methylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006616DM9 repeatSMARTSM00696dm9coord: 78..147
e-value: 1.1E-12
score: 58.1
coord: 4..69
e-value: 1.3E-16
score: 71.2
IPR024518Domain of unknown function DUF3421PFAMPF11901DUF3421coord: 23..136
e-value: 7.6E-33
score: 112.9
coord: 101..146
e-value: 1.3E-10
score: 41.3
NoneNo IPR availablePANTHERPTHR31649FAMILY NOT NAMEDcoord: 5..147