DUF862 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014469 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: G0NDH0 (Uncharacterized protein OS=Caenorhabditis brenneri OX=135651 GN=CAEBREN_19348 PE=4 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 2.296e-9 Identity = 54/173 (31.21%), Postives = 80/173 (46.24%), Query Frame = 3 Query: 108 MAKGLSWEGKIFYVDD-----ITKPKEIYNQ--HEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVV--HHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRS--RVSLLKSNYNVNVDFAKIIQWIIN-DELKRGYYIFNFKINCQGFASCLYSEVTR 590 M+ G W G + + DD I + +Q +L G EI Y+ LD I +I H + +V+TTN+Y+S +K +G+F+Q+SK K + D RS R SN + + KI QWIIN EL Y++ + NC FAS L+ + R Sbjct: 1 MSFGGDWTGCLHFWDDDKKKWIQVSADKVHQFFRNVLKAEGEIIIFSEIRKYD-----LDMIPEWRIPGWFGNWHEYTLVETTNWYWSVEKMAEGLFVQRSKSKNIIRCKFGADDRSKDRSEGRSSNVSFGENLMKIAQWIINRRELGNTYHVLDR--NCHHFASLLHEAIVR 166
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: G0MPJ3 (Uncharacterized protein OS=Caenorhabditis brenneri OX=135651 GN=CAEBREN_06627 PE=4 SV=1) HSP 1 Score: 64.3142 bits (155), Expect = 3.577e-8 Identity = 35/100 (35.00%), Postives = 62/100 (62.00%), Query Frame = 3 Query: 297 VHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSR--VSLLKSNYNVNVDFAKIIQWIIN-----DELKRGYYIFNFKINCQGFASCLY 575 ++HAF V+TTN+++SFDK +GI +Q+SKKK++V G R++ S + ++ + ++ +WII+ +EL + Y++ +K NCQ FA +Y Sbjct: 71 LYHAFTTVETTNWHWSFDKMAEGIHVQRSKKKDEVCSYFEGKMRTKKPSSWRSAKFSNGKNILEVAKWIIDSGTPENELNKIYHV--WKQNCQLFAHTIY 168
BLAST of DUF862 domain-containing protein vs. TrEMBL
Match: A0A3M6TM43 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00021733 PE=4 SV=1) HSP 1 Score: 56.9954 bits (136), Expect = 3.351e-6 Identity = 41/132 (31.06%), Postives = 70/132 (53.03%), Query Frame = 3 Query: 189 EILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQR-SRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSE 581 E L +I + E I+ I Y PL + N K + +H F+V++TTN+Y+S K+ + I +Q+SK K V G R + V+++ N +I++I +EL + Y + + NCQ FA L+++ Sbjct: 59 EDLHRITNSEEIQYIGSYQCPL-----VENMKGHLIAYHQFLVIETTNWYWSIGKDSEKILLQRSKVKNRVEERKEGQARYTPVTMIVENEGRG-SMRDLIKFICENELDKTYDL--LRDNCQQFAERLFNQ 182
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000007684.1 (SMESG000007684.1) HSP 1 Score: 366.696 bits (940), Expect = 7.871e-131 Identity = 179/181 (98.90%), Postives = 181/181 (100.00%), Query Frame = 3 Query: 75 MSFNKSFHNQSMAKGLSWEGKIFYVDDITKPKEIYNQHEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEVTREFIEESLFT 617 MSFNKSFHNQSMAKGLSWEGKIFYVDDITKPKEIYN+HEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHA+VVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEVTREFIEESLFT Sbjct: 1 MSFNKSFHNQSMAKGLSWEGKIFYVDDITKPKEIYNEHEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAYVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEVTREFIEESLFT 181
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000070711.1 (SMESG000070711.1) HSP 1 Score: 248.44 bits (633), Expect = 3.373e-84 Identity = 116/169 (68.64%), Postives = 143/169 (84.62%), Query Frame = 3 Query: 108 MAKGLSWEGKIFYVDDITKPKEIYNQHEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEVTREFIEESLF 614 MAKG +WEGKIFY+DD+ +P+EIYNQHEIL KIGSKETI EI++Y TPLQGLDF+ EK+VHHAFVVVKTTNFYFSFDKNG+GI+IQKS+ K+DVL S+FGDQRSR LKSN NV VDF II+WII DELKRGY+I NF+ NCQGF++ LY++V+++ + + +F Sbjct: 1 MAKGQNWEGKIFYIDDLERPEEIYNQHEILQKIGSKETIVEIDVYKTPLQGLDFVEEKVYEKIVHHAFVVVKTTNFYFSFDKNGRGIYIQKSENKDDVLKSVFGDQRSRPVKLKSNTNVRVDFDTIIKWIIRDELKRGYHILNFETNCQGFSASLYTKVSKQTMMQFIF 169
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024663.1 (SMESG000024663.1) HSP 1 Score: 109.768 bits (273), Expect = 6.306e-30 Identity = 58/167 (34.73%), Postives = 97/167 (58.08%), Query Frame = 3 Query: 90 SFHNQSMAKGLSWEGKIFYVDDITKPKEIYNQHEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEVTR 590 S + ++ KG +WE +I ++++ + P E++N E+ I + ETI ++ Y L + ++HAFV+ +T ++Y+S +KNG GI+IQKSK K++++ I G++R+ +LL S V II+WI +ELK Y+ N NCQGFA Y+ + R Sbjct: 2 SVNPKAQKKGGNWESRIHFINNTSAPNEVFNGSEMRKLIDTSETIVKVWKYKEKLVWWQITS-----FYLYHAFVIFETNDWYWSMEKNGSGIWIQKSKSKDNLVAKIGGNERNSTTLLDSCQGRFVTIKNIIEWIEKEELKSAYWYMNSDDNCQGFADRGYNHIVR 163
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024661.1 (SMESG000024661.1) HSP 1 Score: 94.3597 bits (233), Expect = 5.859e-24 Identity = 49/157 (31.21%), Postives = 88/157 (56.05%), Query Frame = 3 Query: 114 KGLSWEGKIFYVDDITKPKEIYNQHEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEV 584 KG +W +IF++++ + P +++ E+ I + ETI + Y L + ++HAFV+ +T ++Y+S +KNG GI+IQKSK K +++ G++R+ +LL S + + ++I+W+ N+EL Y+ NCQGFA Y + Sbjct: 10 KGGNWLSRIFFINNTSAPNDVFKGSEMRKLIDTSETIIQAWKYKQKLVWFQITS-----FFLYHAFVIFETKDWYWSIEKNGSGIWIQKSKSKNELVTKNGGNERNSTTLLDSEFGNFITIGELIEWVENNELINRYFYIETYFNCQGFADRAYQHI 161
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Match: SMESG000024669.1 (SMESG000024669.1) HSP 1 Score: 85.8853 bits (211), Expect = 7.151e-21 Identity = 46/154 (29.87%), Postives = 82/154 (53.25%), Query Frame = 3 Query: 123 SWEGKIFYVDDITKPKEIYNQHEILPKIGSKETIKEIELYNTPLQGLDFINNAKIEKVVHHAFVVVKTTNFYFSFDKNGQGIFIQKSKKKEDVLLSIFGDQRSRVSLLKSNYNVNVDFAKIIQWIINDELKRGYYIFNFKINCQGFASCLYSEV 584 WEG+IF++ D P E+YN E+ + + I + Y TP+ + + +H F+V++T F++S +KN GI+IQK + K DV+ + G++R+ V L+ F ++ WI +EL++GY ++ N +GF +Y + Sbjct: 7 DWEGRIFFIRDTNCPNELYNGGEMKRQFDLSKPILHVWKYKTPMHWIQLTS-----FYFYHIFIVIETDEFFWSVEKNNLGIWIQKGRFKNDVISKMAGEERNLVICLEDLQGDFGQFRNLVDWIEINELQKGYSCNDYDFNSKGFGDKVYDNI 155 The following BLAST results are available for this feature:
BLAST of DUF862 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of DUF862 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of DUF862 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of DUF862 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014469 ID=SMED30014469|Name=DUF862 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=701bpback to top protein sequence of SMED30014469-orf-1 >SMED30014469-orf-1 ID=SMED30014469-orf-1|Name=SMED30014469-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=182bp MSFNKSFHNQSMAKGLSWEGKIFYVDDITKPKEIYNQHEILPKIGSKETIback to top Annotated Terms
The following terms have been associated with this transcript:
|