bak-2

Overview
Namebak-2 (AEX93475.1)
Smed IDSMED30014459
Length (bp)845
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of bak-2 (SMED30014459) t-SNE clustered cells

Violin plots show distribution of expression levels for bak-2 (SMED30014459) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of bak-2 (SMED30014459) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for bak-2 (SMED30014459) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014459

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30014459SMESG000008222.1 dd_Smed_v4_1767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014459SMESG000008222.1 dd_Smed_v4_1767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014459SMESG000008222.1 dd_Smed_v4_1767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014459SMESG000008222.1 dd_Smed_v4_1767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30014459SMESG000008222.1 dd_Smed_v4_1767_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of bak-2 vs. Ensembl Human
Match: BAK1 (BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:HGNC:949])

HSP 1 Score: 62.003 bits (149), Expect = 5.440e-11
Identity = 41/147 (27.89%), Postives = 65/147 (44.22%), Query Frame = 1 
Query:  262 QVAQD--------IINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            QVAQD        +    +   +     +   L  I D+I++ Y +EF+ ++       +   A N Y +F +I  S+FESGI  GR+  LL FGYR  +         F      L  +  ++V F+    +  +IA  GGW A +
Sbjct:   26 QVAQDTEEVFRSYVFYRHQQQPSSTMGQVGRQLAIIGDDINRRYDSEFQTML-----QHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGF------LGQVTRFVVDFMLHHCIARWIAQRGGWVAAL 161
[Close]
BLAST of bak-2 vs. Ensembl Human
Match: BAK1 (BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:HGNC:949])

HSP 1 Score: 62.003 bits (149), Expect = 6.150e-11
Identity = 36/119 (30.25%), Postives = 57/119 (47.90%), Query Frame = 1 
Query:  322 IASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            +   L  I D+I++ Y +EF+ ++       +   A N Y +F +I  S+FESGI  GR+  LL FGYR  +         F      L  +  ++V F+    +  +IA  GGW A +
Sbjct:   74 VGRQLAIIGDDINRRYDSEFQTML-----QHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGF------LGQVTRFVVDFMLHHCIARWIAQRGGWVAAL 181
[Close]
BLAST of bak-2 vs. Ensembl Zebrafish
Match: zgc:153993 (zgc:153993 [Source:ZFIN;Acc:ZDB-GENE-060929-176])

HSP 1 Score: 50.447 bits (119), Expect = 4.773e-7
Identity = 42/168 (25.00%), Postives = 78/168 (46.43%), Query Frame = 1 
Query:  214 FITSVAQIDIERDKNAQVAQDII-----NETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT 702
            ++   A ID   D N  V+ + +     +E +P        +   L +IAD+++K+   E ++L+     S +  N A     F+ +  SIF+ GI  GR+  L    YR I ++L+         +  ++ II + ++F+    + A+I   GGW+ I  S++   T
Sbjct:   40 YVIQQATID---DPNIHVSHETLGGRPQDEEDP----QIKEVVDQLLKIADDLNKN--AELQHLI-----STVQSNCAQDV--FMTVARSIFDDGINWGRVVALFHLAYRLIFQALT------QNHLEIIKNIISWFLQFMKKH-ISAWIRQQGGWEGIFRSMTRWRT 184
[Close]
BLAST of bak-2 vs. Ensembl Xenopus
Match: rmdn1 (regulator of microtubule dynamics 1 [Source:Xenbase;Acc:XB-GENE-974332])

HSP 1 Score: 49.6766 bits (117), Expect = 8.868e-7
Identity = 32/130 (24.62%), Postives = 62/130 (47.69%), Query Frame = 1 
Query:  292 EPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNP-LQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            EP     C+ + S ++   DE ++ +   F       I+++I       Y  F E+  S+F+ G+  GRI    +FG        ++ AE+ + +++P L  I  ++V +L +  ++ +I S GGW   +
Sbjct:   28 EPAGAASCS-LHSAMRAAGDEFEERFRQAFSE-----ISTQIHVTPGTAYARFAEVAGSLFQGGVNWGRIVAFFVFG-------AALCAESVNKEMSPLLPRIQDWMVTYLET-NLRDWIQSNGGWNGFL 143
[Close]
BLAST of bak-2 vs. Ensembl Mouse
Match: Bak1 (BCL2-antagonist/killer 1 [Source:MGI Symbol;Acc:MGI:1097161])

HSP 1 Score: 68.1662 bits (165), Expect = 3.115e-13
Identity = 51/190 (26.84%), Postives = 84/190 (44.21%), Query Frame = 1 
Query:  115 MASGDTNDRIQDDDDENYTVPEQE--SNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            MASG      +   DE+ +  EQ+   +T+     ++  + Q + E    A  A   ++      N     +   L  I D+I++ Y TEF+NL+      ++   A N Y  F +I +S+F+SGI+ GR+  LL FGYR  +         F      L  +  +L   +    +  +IA  GGW A +
Sbjct:    1 MASGQGPGPPKVGCDESPSPSEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLPLEPNSILGQVGRQLALIGDDINRRYDTEFQNLL-----EQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGF------LGQVTCFLADIILHHYIARWIAQRGGWVAAL 179
[Close]
BLAST of bak-2 vs. UniProt/SwissProt
Match: sp|O08734|BAK_MOUSE (Bcl-2 homologous antagonist/killer OS=Mus musculus OX=10090 GN=Bak1 PE=1 SV=3)

HSP 1 Score: 68.1662 bits (165), Expect = 2.179e-12
Identity = 51/190 (26.84%), Postives = 84/190 (44.21%), Query Frame = 1 
Query:  115 MASGDTNDRIQDDDDENYTVPEQE--SNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            MASG      +   DE+ +  EQ+   +T+     ++  + Q + E    A  A   ++      N     +   L  I D+I++ Y TEF+NL+      ++   A N Y  F +I +S+F+SGI+ GR+  LL FGYR  +         F      L  +  +L   +    +  +IA  GGW A +
Sbjct:    1 MASGQGPGPPKVGCDESPSPSEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLPLEPNSILGQVGRQLALIGDDINRRYDTEFQNLL-----EQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGF------LGQVTCFLADIILHHYIARWIAQRGGWVAAL 179
[Close]
BLAST of bak-2 vs. UniProt/SwissProt
Match: sp|Q16611|BAK_HUMAN (Bcl-2 homologous antagonist/killer OS=Homo sapiens OX=9606 GN=BAK1 PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.953e-10
Identity = 36/119 (30.25%), Postives = 57/119 (47.90%), Query Frame = 1 
Query:  322 IASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            +   L  I D+I++ Y +EF+ ++       +   A N Y +F +I  S+FESGI  GR+  LL FGYR  +         F      L  +  ++V F+    +  +IA  GGW A +
Sbjct:   74 VGRQLAIIGDDINRRYDSEFQTML-----QHLQPTAENAYEYFTKIATSLFESGINWGRVVALLGFGYRLALHVYQHGLTGF------LGQVTRFVVDFMLHHCIARWIAQRGGWVAAL 181
[Close]
BLAST of bak-2 vs. TrEMBL
Match: H2DL13 (Bak-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 402.905 bits (1034), Expect = 2.928e-140
Identity = 195/196 (99.49%), Postives = 196/196 (100.00%), Query Frame = 1 
Query:  115 MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT 702
            MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIIC+AANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT
Sbjct:    1 MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICDAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT 196
[Close]
BLAST of bak-2 vs. TrEMBL
Match: H2DL14 (Bak-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 7.124e-12
Identity = 51/182 (28.02%), Postives = 92/182 (50.55%), Query Frame = 1 
Query:  151 DDDENYTVPEQESNTQNFVTQFITSV-AQIDIERDKNAQVAQDI-----INETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            +D++N    E ESN   ++ Q I  + A+ DI ++ N + A+ +     IN   P N    +  A  LK+I  +++K    E + LV      K+     N Y  FV I  S+FE G+++ ++ +L++FGY+W  +     A + S+       ++ +L  FL S  +++F+   GGWK ++
Sbjct:    6 NDNDNIHFAEAESN---YLLQEIFHIEAENDISQNNNLEPAEIVLSATAINTNNPFN---SSEYADRLKKILSDVEK----EIDELVNN--FEKMTETHQNAYNSFVAIANSLFEDGVSVSKLIILIVFGYKWFTKCHRSIANSISI-------VMKFLYSFLMSDRIKSFVILHGGWKKLL 168
[Close]
BLAST of bak-2 vs. TrEMBL
Match: A0A401S1P3 (Uncharacterized protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0002723 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 9.861e-12
Identity = 48/147 (32.65%), Postives = 71/147 (48.30%), Query Frame = 1 
Query:  244 ERDKNAQVAQD--IINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            ER++ A V +D  II   +P  N     +   L  I D+I++ Y  EF NL+     S +  NA N Y +F +I   +FESGI  GR+  LL FGYR  +       + F      L  I  ++  F+    +  +IA+ GGW A +
Sbjct:   61 EREEGANVPEDPEIIEMQQPPEN-TPTLVGRQLALIGDDINRRYDLEFHNLL-----STLPLNAGNAYDYFRKIADGLFESGINWGRVIALLGFGYRMAIYVFQKGMKGF------LSQIAKFIAEFVLKNRIARWIAAQGGWVAAL 195
[Close]
BLAST of bak-2 vs. TrEMBL
Match: Q9JK59 (BAK protein OS=Rattus norvegicus OX=10116 GN=Bak1 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.829e-11
Identity = 52/190 (27.37%), Postives = 84/190 (44.21%), Query Frame = 1 
Query:  115 MASGDTNDRIQDDDDENYTVPEQE--SNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            MASG      + D DE  +  EQ+   +T+     ++  + Q + E    A  A   ++      N     +   L  I D+I++ Y TEF+NL+      ++   A N Y  F +I +S+F+SGI+ GR+  LL FGYR  +         F      L  +  +L   +    +  +IA  GGW A +
Sbjct:    1 MASGQGPGPPKGDCDEALSASEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLSLEPNSVLGQVGRQLALIGDDINRRYDTEFQNLL-----EQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYVYQRGLTGF------LGQVTCFLADIILHHYIARWIAQRGGWVAAL 179
[Close]
BLAST of bak-2 vs. TrEMBL
Match: W4YE49 (Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.482e-11
Identity = 54/189 (28.57%), Postives = 86/189 (45.50%), Query Frame = 1 
Query:  115 MASGDTN--DRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNP---IASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGW 666
            MA+G +     +Q+D +EN  + + E   +NF  Q +    Q   ++  +  ++   I E +    D  +P   +   L +I D+ID  Y  EF  ++       +    +  Y+ F  +   +F  GI  GRI  LLMFGYR I R +  +   F         I+G LVRF+ S  +  +IA  GGW
Sbjct:    1 MATGTSTITQTVQEDTEEN-VIEQTEMIVRNFFHQRLAMDMQ---DQTNDINISTPRIPELQNFTADPLSPTSRVGRRLAQIGDQIDAQYEGEFRQMI-----QMLHITPSTAYQAFAGVARRLFIKGINWGRISALLMFGYR-IARDVMTSVGDF------FHKIVGDLVRFIISERIATWIAQQGGW 173
[Close]
BLAST of bak-2 vs. Ensembl Nematostella
Match: EDO49892 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF38])

HSP 1 Score: 63.1586 bits (152), Expect = 3.661e-12
Identity = 49/179 (27.37%), Postives = 78/179 (43.58%), Query Frame = 1 
Query:  151 DDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKR---IADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            +D E   V E E   + F+ Q     A+      +  +   D + E    N+     IA+  +R   + DEID  Y  +F +++       +       Y  F  +   +F SGI+ GRI  LL FGY     +++V    FS     L+ II ++V F+    +  +IA  GGW A+M
Sbjct:   19 EDSEKTVVSETEGVFRYFMYQRFQDEAERGDTEVQFTEARSDALEELRSCNDTSRQHIATIGRRLGEVGDEIDAKYRDQFHDMI-----ESLHLTPGTAYDTFAAVARRLFRSGISWGRIIALLCFGYEI---AITVIRRGFSGSF--LRRIIRFVVDFIFRERIARWIAEHGGWVAVM 187
[Close]
BLAST of bak-2 vs. Ensembl Nematostella
Match: EDO37464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFS9])

HSP 1 Score: 58.9214 bits (141), Expect = 1.095e-10
Identity = 41/146 (28.08%), Postives = 72/146 (49.32%), Query Frame = 1 
Query:  265 VAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT 702
            +A+D+I+     N    N  A  L+++AD+I++ Y     +L  K   S+     +  Y  FVEI   +F  G+  GRI VL  FG R     L+V  E  ++  + +Q++  ++ +++  AG+  +IA+ GGW  +        T
Sbjct:   22 LAKDLISYRLGKNPKPPNANAETLRKLADKIEEQYPALLNHLCHKLNISR-----STAYSTFVEIAGEVFADGVNWGRIVVLYAFGGR-----LAVYCEQHNMK-DLVQSVTDWVGKYI--AGLSEWIANEGGWDGLKEQFRDNNT 154
[Close]
BLAST of bak-2 vs. Planmine SMEST
Match: SMESG000008222.1 (SMESG000008222.1)

HSP 1 Score: 402.905 bits (1034), Expect = 8.142e-144
Identity = 195/196 (99.49%), Postives = 196/196 (100.00%), Query Frame = 1 
Query:  115 MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT 702
            MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIIC+AANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT
Sbjct:    1 MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQVAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICDAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKAT 196
[Close]
BLAST of bak-2 vs. Planmine SMEST
Match: SMESG000023905.1 (SMESG000023905.1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.791e-16
Identity = 54/193 (27.98%), Postives = 97/193 (50.26%), Query Frame = 1 
Query:  133 NDRIQD-----DDDENYTVPEQESNTQNFVTQFITSV-AQIDIERDKNAQVAQDI-----INETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            NDR  +     +D++N    E ESN   ++ Q I  + A+ DI ++ N + A+ +     IN   P N+      A  LK+I  +++K    E + LV      K+     N Y +FV I  S+FE G+++ ++ +L++FGY+W  +     A + S+       ++ +L  FL S  +++F+   GGWK ++
Sbjct:    6 NDRFLNMASKYNDNDNIHCAEPESN---YLLQEIFHIEAENDISQNNNLEPAEIVLSATTINTNNPFNSSA---YADRLKKILSDVEK----EIDELVNN--FEKMTETHQNAYNNFVAIANSLFEDGVSVSKLIILIVFGYKWFTKCHKSIANSISI-------VMKFLYSFLMSDRIRSFVILHGGWKKLL 179
[Close]
BLAST of bak-2 vs. Planmine SMEST
Match: SMESG000023905.1 (SMESG000023905.1)

HSP 1 Score: 73.9442 bits (180), Expect = 7.032e-16
Identity = 51/182 (28.02%), Postives = 93/182 (51.10%), Query Frame = 1 
Query:  151 DDDENYTVPEQESNTQNFVTQFITSV-AQIDIERDKNAQVAQDI-----INETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSKIICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAETFSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIM 678
            +D++N    E ESN   ++ Q I  + A+ DI ++ N + A+ +     IN   P N+      A  LK+I  +++K    E + LV      K+     N Y +FV I  S+FE G+++ ++ +L++FGY+W  +     A + S+       ++ +L  FL S  +++F+   GGWK ++
Sbjct:    6 NDNDNIHCAEPESN---YLLQEIFHIEAENDISQNNNLEPAEIVLSATTINTNNPFNSSA---YADRLKKILSDVEK----EIDELVNN--FEKMTETHQNAYNNFVAIANSLFEDGVSVSKLIILIVFGYKWFTKCHKSIANSISI-------VMKFLYSFLMSDRIRSFVILHGGWKKLL 168
The following BLAST results are available for this feature:
BLAST of bak-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
BAK15.440e-1127.89BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:H... [more]
BAK16.150e-1130.25BCL2 antagonist/killer 1 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of bak-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
zgc:1539934.773e-725.00zgc:153993 [Source:ZFIN;Acc:ZDB-GENE-060929-176][more]
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BLAST of bak-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rmdn18.868e-724.62regulator of microtubule dynamics 1 [Source:Xenbas... [more]
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BLAST of bak-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Bak13.115e-1326.84BCL2-antagonist/killer 1 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of bak-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|O08734|BAK_MOUSE2.179e-1226.84Bcl-2 homologous antagonist/killer OS=Mus musculus... [more]
sp|Q16611|BAK_HUMAN2.953e-1030.25Bcl-2 homologous antagonist/killer OS=Homo sapiens... [more]
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BLAST of bak-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H2DL132.928e-14099.49Bak-2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
H2DL147.124e-1228.02Bak-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
A0A401S1P39.861e-1232.65Uncharacterized protein OS=Chiloscyllium punctatum... [more]
Q9JK592.829e-1127.37BAK protein OS=Rattus norvegicus OX=10116 GN=Bak1 ... [more]
W4YE494.482e-1128.57Uncharacterized protein OS=Strongylocentrotus purp... [more]
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BLAST of bak-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO498923.661e-1227.37Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO374641.095e-1028.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of bak-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of bak-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000008222.18.142e-14499.49SMESG000008222.1[more]
SMESG000023905.16.791e-1627.98SMESG000023905.1[more]
SMESG000023905.17.032e-1628.02SMESG000023905.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30014459
GenBankAEX93475.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014459 ID=SMED30014459|Name=bak-2|organism=Schmidtea mediterranea sexual|type=transcript|length=845bp
GTTTGTTTAAAAATTCAAGCTAAAAAATATTATTTTAAAATTTAAAACAA
TTCTAATTTTTACCATCATTATCATTTCTGAATCTTTTTATTTTGTTAAG
TGTATTTAATTTTTATGGCATCGGGAGATACCAATGATAGAATTCAAGAT
GATGATGATGAAAATTATACAGTGCCGGAACAAGAGTCTAATACTCAAAA
TTTTGTAACGCAATTTATTACCTCGGTCGCCCAAATTGATATTGAACGCG
ATAAAAATGCTCAAGTTGCACAAGATATTATTAATGAAACGGAGCCATTA
AATAATGATTGTTGCAACCCAATCGCATCTGATTTGAAAAGAATAGCAGA
TGAAATTGATAAATCATATGGAACGGAGTTTGAAAATTTAGTAACTAAAG
CTATAACATCAAAGATCATATGTAACGCGGCAAATGGGTACAGGCATTTT
GTAGAAATTACAGCTAGCATATTTGAAAGTGGAATAACGCTAGGAAGAAT
CTATGTTTTATTGATGTTTGGTTATCGATGGATTGTCCGCAGTTTGTCAG
TTGCTGCCGAAACATTTTCAGTGGACGTTAATCCCTTACAAACCATTATA
GGGTATTTAGTGCGATTTCTTTCCAGTGCTGGTGTTCAGGCTTTTATTGC
AAGTATTGGTGGCTGGAAAGCAATCATGCATTCTCTTAGTTCGAAAGCCA
CTATAATATTTGCCGCCTTAGCTGGTGTGTTTGTATTCCTCGCGGTGATT
TTTAACAAATAAACTATTTGATATGAATTTGTTAAATTTTAATGTATTTT
GTCAAACGATATAGTTCAATATATTTTGTATTCAGGCAAAAAAAA
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protein sequence of SMED30014459-orf-1

>SMED30014459-orf-1 ID=SMED30014459-orf-1|Name=SMED30014459-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=216bp
MASGDTNDRIQDDDDENYTVPEQESNTQNFVTQFITSVAQIDIERDKNAQ
VAQDIINETEPLNNDCCNPIASDLKRIADEIDKSYGTEFENLVTKAITSK
IICNAANGYRHFVEITASIFESGITLGRIYVLLMFGYRWIVRSLSVAAET
FSVDVNPLQTIIGYLVRFLSSAGVQAFIASIGGWKAIMHSLSSKATIIFA
ALAGVFVFLAVIFNK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036834Blc2-like_sf
IPR026298Blc2_fam
IPR002475Bcl2-like
Vocabulary: biological process
TermDefinition
GO:0042981regulation of apoptotic process
GO:0043065positive regulation of apoptotic process
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026298Blc2 familyPFAMPF00452Bcl-2coord: 74..184
e-value: 8.6E-10
score: 39.2
IPR026298Blc2 familyPANTHERPTHR11256BCL-2 RELATEDcoord: 20..192
IPR036834Blc2-like superfamilyGENE3DG3DSA:1.10.437.10coord: 14..189
e-value: 8.2E-24
score: 86.2
IPR036834Blc2-like superfamilySUPERFAMILYSSF56854Bcl-2 inhibitors of programmed cell deathcoord: 61..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR11256:SF56coord: 20..192
NoneNo IPR availableTMHMMTMhelixcoord: 190..212
NoneNo IPR availableTMHMMTMhelixcoord: 161..183
NoneNo IPR availableTMHMMTMhelixcoord: 119..141
IPR002475Bcl2-likePROSITEPS50062BCL2_FAMILYcoord: 74..186
score: 15.454