SMED30014423
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014423 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of SMED30014423 vs. Ensembl Nematostella
Match: EDO40301 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7U5]) HSP 1 Score: 46.9802 bits (110), Expect = 4.211e-6 Identity = 21/45 (46.67%), Postives = 28/45 (62.22%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 V +G G GWV +G GYGWVW+G+ GWV +G G V +G+ Sbjct: 790 VSLGIAGYGWVSLGIAGYGWVWLGIAGYGWVSLGMVGYRWVWLGI 834
BLAST of SMED30014423 vs. Planmine SMEST
Match: SMESG000046838.1 (SMESG000046838.1) HSP 1 Score: 63.1586 bits (152), Expect = 2.996e-11 Identity = 31/45 (68.89%), Postives = 36/45 (80.00%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 +RMGTDG GW+RMG DGYGW+ +G D GWVRMG+DG G VRMG Sbjct: 57 IRMGTDGYGWIRMGTDGYGWIRMGTDGYGWVRMGSDGYGWVRMGT 101 HSP 2 Score: 62.003 bits (149), Expect = 8.086e-11 Identity = 30/45 (66.67%), Postives = 35/45 (77.78%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 +RMGTDG GWVRMG+DGYGWV +G D GW+RM DG G +RMG Sbjct: 77 IRMGTDGYGWVRMGSDGYGWVRMGTDGYGWIRMNTDGYGWIRMGT 121 HSP 3 Score: 61.2326 bits (147), Expect = 1.491e-10 Identity = 30/45 (66.67%), Postives = 34/45 (75.56%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 +RMGTDG GW+RMG DGYGWV +G D GWVRMG DG G +RM Sbjct: 67 IRMGTDGYGWIRMGTDGYGWVRMGSDGYGWVRMGTDGYGWIRMNT 111 HSP 4 Score: 58.5362 bits (140), Expect = 1.172e-9 Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRM 129 VRMG+DG GWVRMG DGYGW+ + D GW+RMG DG G +RM Sbjct: 87 VRMGSDGYGWVRMGTDGYGWIRMNTDGYGWIRMGTDGYGWIRM 129 HSP 5 Score: 58.151 bits (139), Expect = 1.633e-9 Identity = 28/44 (63.64%), Postives = 33/44 (75.00%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGWVRMG+DG WVRMG DGYG + + DG GW+RMG D GW+ Sbjct: 84 YGWVRMGSDGYGWVRMGTDGYGWIRMNTDGYGWIRMGTDGYGWI 127 HSP 6 Score: 57.7658 bits (138), Expect = 2.134e-9 Identity = 29/44 (65.91%), Postives = 33/44 (75.00%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMG 132 VRM T G GW+RMG DGYGW+ +G D GW+RMG DG G VRMG Sbjct: 47 VRMSTVGYGWIRMGTDGYGWIRMGTDGYGWIRMGTDGYGWVRMG 90 HSP 7 Score: 56.9954 bits (136), Expect = 3.322e-9 Identity = 28/44 (63.64%), Postives = 33/44 (75.00%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGW+RMG DG W+RMG DGYG + + DG GWVRMG+D GWV Sbjct: 54 YGWIRMGTDGYGWIRMGTDGYGWIRMGTDGYGWVRMGSDGYGWV 97 HSP 8 Score: 56.9954 bits (136), Expect = 4.420e-9 Identity = 28/45 (62.22%), Postives = 33/45 (73.33%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 +RMGTDG GWVRM GYGW+ +G D GW+RMG DG G +RMG Sbjct: 37 LRMGTDGYGWVRMSTVGYGWIRMGTDGYGWIRMGTDGYGWIRMGT 81 HSP 9 Score: 56.225 bits (134), Expect = 7.678e-9 Identity = 28/44 (63.64%), Postives = 32/44 (72.73%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGW+RMG DG W+RMG DGYG V + DG GWVRMG D GW+ Sbjct: 64 YGWIRMGTDGYGWIRMGTDGYGWVRMGSDGYGWVRMGTDGYGWI 107 HSP 10 Score: 55.8398 bits (133), Expect = 9.574e-9 Identity = 28/45 (62.22%), Postives = 33/45 (73.33%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 VR+GTDG GW+RMG DGYGWV + GW+RMG DG G +RMG Sbjct: 27 VRIGTDGYGWLRMGTDGYGWVRMSTVGYGWIRMGTDGYGWIRMGT 71 HSP 11 Score: 55.4546 bits (132), Expect = 1.205e-8 Identity = 28/43 (65.12%), Postives = 32/43 (74.42%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 TDGYGW+RMG +G GWV MG D YG V MG DG GW+R+ DG Sbjct: 71 TDGYGWIRMGTDGYGWVRMGSDGYGWVRMGTDGYGWIRMNTDG 113 HSP 12 Score: 55.4546 bits (132), Expect = 1.309e-8 Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 TDGYGW+RMG +G GW+ MG D YG V MG+DG GWVR+ DG Sbjct: 61 TDGYGWIRMGTDGYGWIRMGTDGYGWVRMGSDGYGWVRMGTDG 103 HSP 13 Score: 55.0694 bits (131), Expect = 1.805e-8 Identity = 27/44 (61.36%), Postives = 31/44 (70.45%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGW+RMG DG WVRMG DGYG V + DG GW+RM D GW+ Sbjct: 74 YGWIRMGTDGYGWVRMGSDGYGWVRMGTDGYGWIRMNTDGYGWI 117 HSP 14 Score: 53.9138 bits (128), Expect = 3.937e-8 Identity = 27/43 (62.79%), Postives = 32/43 (74.42%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 TDGYGWVRMG++G GWV MG D YG + M DG GW+R+ DG Sbjct: 81 TDGYGWVRMGSDGYGWVRMGTDGYGWIRMNTDGYGWIRMGTDG 123 HSP 15 Score: 53.1434 bits (126), Expect = 6.761e-8 Identity = 27/45 (60.00%), Postives = 32/45 (71.11%), Query Frame = 1 Query: 1 VRMGTDGCGWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGV 135 +RM TDG GWVR+G DGYGW+ +G D GWVRM G G +RMG Sbjct: 17 LRMDTDGYGWVRIGTDGYGWLRMGTDGYGWVRMSTVGYGWIRMGT 61 HSP 16 Score: 52.373 bits (124), Expect = 1.226e-7 Identity = 26/44 (59.09%), Postives = 31/44 (70.45%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGWVR+G DG W+RMG DGYG V + G GW+RMG D GW+ Sbjct: 24 YGWVRIGTDGYGWLRMGTDGYGWVRMSTVGYGWIRMGTDGYGWI 67 HSP 17 Score: 51.6026 bits (122), Expect = 2.367e-7 Identity = 26/43 (60.47%), Postives = 30/43 (69.77%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 TDGYGWVRM G GW+ MG D YG + MG DG GW+R+ DG Sbjct: 41 TDGYGWVRMSTVGYGWIRMGTDGYGWIRMGTDGYGWIRMGTDG 83 HSP 18 Score: 51.6026 bits (122), Expect = 2.389e-7 Identity = 26/44 (59.09%), Postives = 30/44 (68.18%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGWVRM G W+RMG DGYG + + DG GW+RMG D GWV Sbjct: 44 YGWVRMSTVGYGWIRMGTDGYGWIRMGTDGYGWIRMGTDGYGWV 87 HSP 19 Score: 51.6026 bits (122), Expect = 2.501e-7 Identity = 26/43 (60.47%), Postives = 32/43 (74.42%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 T GYGW+RMG +G GW+ MG D YG + MG DG GWVR+ +DG Sbjct: 51 TVGYGWIRMGTDGYGWIRMGTDGYGWIRMGTDGYGWVRMGSDG 93 HSP 20 Score: 50.447 bits (119), Expect = 5.143e-7 Identity = 25/44 (56.82%), Postives = 30/44 (68.18%), Query Frame = 2 Query: 2 YGWVRMGADGCEWVRMGMDGYGSVWICVDGCGWVRMGADLCGWV 133 YGW+RMG DG WVRM GYG + + DG GW+RMG D GW+ Sbjct: 34 YGWLRMGTDGYGWVRMSTVGYGWIRMGTDGYGWIRMGTDGYGWI 77 HSP 21 Score: 49.2914 bits (116), Expect = 1.427e-6 Identity = 28/47 (59.57%), Postives = 31/47 (65.96%), Query Frame = 1 Query: 1 VRMGTDGC-GWVRMGADGYGWVWIGMDLCGWVRMGADGCGSVRMGVC 138 +RM TDG G +RM DGYGWV IG D GW+RMG DG G VRM Sbjct: 6 IRMETDGYDGRLRMDTDGYGWVRIGTDGYGWLRMGTDGYGWVRMSTV 52 HSP 22 Score: 48.9062 bits (115), Expect = 1.650e-6 Identity = 25/43 (58.14%), Postives = 30/43 (69.77%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 TDGYGW+RMG +G GWV M YG + MG DG GW+R+ DG Sbjct: 31 TDGYGWLRMGTDGYGWVRMSTVGYGWIRMGTDGYGWIRMGTDG 73 HSP 23 Score: 48.1358 bits (113), Expect = 3.639e-6 Identity = 25/43 (58.14%), Postives = 30/43 (69.77%), Query Frame = 3 Query: 3 TDGYGWVRMGANGCGWVWMGMDRYGSVWMGADGCGWVRICADG 131 TDGYGWVR+G +G GW+ MG D YG V M G GW+R+ DG Sbjct: 21 TDGYGWVRIGTDGYGWLRMGTDGYGWVRMSTVGYGWIRMGTDG 63
BLAST of SMED30014423 vs. Planmine SMEST
Match: SMESG000038129.1 (SMESG000038129.1) HSP 1 Score: 62.003 bits (149), Expect = 7.969e-11 Identity = 36/58 (62.07%), Postives = 41/58 (70.69%), Query Frame = -1 Query: 6 EPLVLKHFTHT-----PIRTDPHPSAPIRTHPHRSIPIHTHPYPSAPIRTHPHPSVPI 164 EPLVLKHFTHT IRTDPH S PI T P+RSIPI+T P S P+ T P+ S+PI Sbjct: 473 EPLVLKHFTHTNQRSIQIRTDPHKSIPIHTDPYRSIPINTGPCRSIPVHTGPYRSIPI 530 HSP 2 Score: 51.2174 bits (121), Expect = 3.390e-7 Identity = 27/42 (64.29%), Postives = 30/42 (71.43%), Query Frame = -2 Query: 2 SAQIRTHPHPSAPIHTDPYRSIPIHTHPHPFAPIRTHPYPSV 127 S QIRT PH S PIHTDPYRSIPI+T P P+ T PY S+ Sbjct: 487 SIQIRTDPHKSIPIHTDPYRSIPINTGPCRSIPVHTGPYRSI 528 HSP 3 Score: 48.521 bits (114), Expect = 2.820e-6 Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = -2 Query: 2 IRTHPHPSAPIHTDPYRSIPIHTHPHPFAPIRTHPYPSV 118 I T P+ S PIHTDPYRSIPIHT P+ + PI T PY S+ Sbjct: 530 IHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSI 568 HSP 4 Score: 48.521 bits (114), Expect = 2.820e-6 Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = -2 Query: 2 IRTHPHPSAPIHTDPYRSIPIHTHPHPFAPIRTHPYPSV 118 I T P+ S PIHTDPYRSIPIHT P+ + PI T PY S+ Sbjct: 650 IHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSI 688 HSP 5 Score: 48.1358 bits (113), Expect = 3.116e-6 Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = -1 Query: 6 FTHTPIRTDPHPSAPIRTHPHRSIPIHTHPYPSAPIRTHPHPSVPI 143 + + PI TDP+ S PI T P+RSIPIHT PY PI T P+ S+PI Sbjct: 645 YRYIPIHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSIPI 690
BLAST of SMED30014423 vs. Planmine SMEST
Match: SMESG000038129.1 (SMESG000038129.1) HSP 1 Score: 48.521 bits (114), Expect = 2.729e-6 Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = -2 Query: 2 IRTHPHPSAPIHTDPYRSIPIHTHPHPFAPIRTHPYPSV 118 I T P+ S PIHTDPYRSIPIHT P+ + PI T PY S+ Sbjct: 510 IHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSI 548 HSP 2 Score: 48.521 bits (114), Expect = 2.729e-6 Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = -2 Query: 2 IRTHPHPSAPIHTDPYRSIPIHTHPHPFAPIRTHPYPSV 118 I T P+ S PIHTDPYRSIPIHT P+ + PI T PY S+ Sbjct: 630 IHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSI 668 HSP 3 Score: 48.1358 bits (113), Expect = 3.155e-6 Identity = 26/42 (61.90%), Postives = 30/42 (71.43%), Query Frame = -1 Query: 6 PIRTDPHPSAPIRTHPHRSIPIHTHPYPSAPIRTHPHPSVPI 131 PI TDP+ S PI T P RSIP+HT PY S PI T P+ S+PI Sbjct: 479 PIHTDPYRSIPINTGPCRSIPVHTGPYRSIPIHTDPYRSIPI 520 HSP 4 Score: 47.7506 bits (112), Expect = 4.787e-6 Identity = 26/46 (56.52%), Postives = 32/46 (69.57%), Query Frame = -1 Query: 6 FTHTPIRTDPHPSAPIRTHPHRSIPIHTHPYPSAPIRTHPHPSVPI 143 + + PI TDP+ S PI T P+RSIPIHT PY PI T P+ S+PI Sbjct: 625 YRYIPIHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSIPI 670 HSP 5 Score: 46.9802 bits (110), Expect = 8.467e-6 Identity = 26/42 (61.90%), Postives = 30/42 (71.43%), Query Frame = -1 Query: 6 PIRTDPHPSAPIRTHPHRSIPIHTHPYPSAPIRTHPHPSVPI 131 PI TDP+ S PI T P+RSIPIHT PY PI T P+ S+PI Sbjct: 509 PIHTDPYRSIPIHTDPYRSIPIHTDPYRYIPIHTDPYRSIPI 550 The following BLAST results are available for this feature:
BLAST of SMED30014423 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30014423 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30014423 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30014423 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30014423 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30014423 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014423 ID=SMED30014423|Name=SMED30014423|organism=Schmidtea mediterranea sexual|type=transcript|length=764bpback to top Annotated Terms
The following terms have been associated with this transcript:
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