SMED30014416

Overview
NameSMED30014416
Smed IDSMED30014416
Length (bp)710
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014416 (SMED30014416) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014416 (SMED30014416) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014416 (SMED30014416) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014416 (SMED30014416) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014416

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30014416SMESG000023005.1 Contig4394newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014416SMESG000023005.1 Contig4394uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014416SMESG000038746.1 Contig4394newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014416SMESG000038746.1 Contig4394uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30014416SMESG000023005.1 Contig16227newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014416SMESG000023005.1 Contig16227uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014416SMESG000023005.1 Contig4394newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014416SMESG000023005.1 Contig4394uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014416SMESG000038746.1 Contig4394newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014416SMESG000038746.1 Contig4394uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30014416SMESG000023005.1 dd_Smed_v6_1571_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30014416SMESG000023005.1 dd_Smed_v6_1571_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014416SMESG000023005.1 dd_Smed_v6_1571_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014416 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002725:41168..41876 -3470
v31.002725:42632..43333 -2608
Homology
[Close]
BLAST of SMED30014416 vs. Planmine SMEST
Match: SMESG000023005.1 (SMESG000023005.1)

HSP 1 Score: 335.88 bits (860), Expect = 4.816e-117
Identity = 184/188 (97.87%), Postives = 186/188 (98.94%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVGTPRLDADPEVSCQTGHLIMDSG+QTDIDD KDKIIITAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGKSSKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMG+KEQNLE SRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI
Sbjct: 3961 KKLQVGTPRLDADPEVSCQTGHLIMDSGDQTDIDDIKDKIIITAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGKSSKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGNKEQNLEVSRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 4148

HSP 2 Score: 104.375 bits (259), Expect = 4.816e-117
Identity = 49/51 (96.08%), Postives = 51/51 (100.00%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKK+
Sbjct: 3913 EVDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKL 3963

HSP 3 Score: 271.552 bits (693), Expect = 5.447e-89
Identity = 153/188 (81.38%), Postives = 166/188 (88.30%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG+ RL  D EVSCQTGHLIM+S  QT+ID+  D + IT   +D+S  ++LF+E    F+KEIQFETTE+SYSEH K+SKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYD EVIIVTNAMQKEVKGKKLQVGT KLEGDLEVSCQTGHLI
Sbjct: 3313 KKLQVGSARLYDDFEVSCQTGHLIMNSECQTEIDNEIDTLTITMHSKDYS--ERLFREPIKYFEKEIQFETTENSYSEHVKASKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDDEVIIVTNAMQKEVKGKKLQVGTSKLEGDLEVSCQTGHLI 3498

HSP 4 Score: 75.0998 bits (183), Expect = 5.447e-89
Identity = 34/51 (66.67%), Postives = 44/51 (86.27%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMIME KERNLE SLF +DLIDE+TQ+D +NL+ T+A  K++KGKK+
Sbjct: 3265 EVDEFMIMERKERNLEESLFFDDLIDESTQFDGENLNVTVAVPKEIKGKKL 3315

HSP 5 Score: 271.552 bits (693), Expect = 1.878e-88
Identity = 153/188 (81.38%), Postives = 166/188 (88.30%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG+ RL  D EVSCQTGHLIM+S  QT+ID+  D + IT   +D+S  ++LF+E    F+KEIQFETTE+SYSEH K+SKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYD EVIIVTNAMQKEVKGKKLQVGT KLEGDLEVSCQTGHLI
Sbjct: 3637 KKLQVGSARLYDDFEVSCQTGHLIMNSECQTEIDNEIDTLTITMHSKDYS--ERLFREPIKYFEKEIQFETTENSYSEHVKASKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDDEVIIVTNAMQKEVKGKKLQVGTSKLEGDLEVSCQTGHLI 3822

HSP 6 Score: 73.1738 bits (178), Expect = 1.878e-88
Identity = 33/51 (64.71%), Postives = 44/51 (86.27%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E++EFMIME KERNLE SLF +DLIDE+TQ+D +NL+ T+A  K++KGKK+
Sbjct: 3589 EVEEFMIMERKERNLEESLFFDDLIDESTQFDGENLNVTVAVPKEIKGKKL 3639

HSP 7 Score: 224.557 bits (571), Expect = 1.348e-70
Identity = 130/190 (68.42%), Postives = 152/190 (80.00%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDF--SVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVGT +L+ D EVSCQTGHLI+DS  QT+ DD  D + IT   +DF  + ED L    K   +KEIQFETTEDSYSEH K++KSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEV+E MIM +KE+NLEAS  ++DLI+E+TQYD + +  T A  K+VKGKKLQVGTP+L+ D EVSCQTGHLI
Sbjct: 3799 KKLQVGTSKLEGDLEVSCQTGHLIIDSECQTEFDDLSDTVTITLNSKDFGENKEDSL----KEYLEKEIQFETTEDSYSEHVKANKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKLQVGTPRLDADPEVSCQTGHLI 3984

HSP 8 Score: 60.8474 bits (146), Expect = 1.348e-70
Identity = 29/51 (56.86%), Postives = 41/51 (80.39%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E++E MIM +KE+NLEAS  ++DLI+E+TQYDD+ +  T A  K+VKGKK+
Sbjct: 3751 EVNEIMIMGSKEQNLEASRLIDDLIEESTQYDDEVIIVTNAMQKEVKGKKL 3801

HSP 9 Score: 223.016 bits (567), Expect = 3.833e-70
Identity = 131/190 (68.95%), Postives = 149/190 (78.42%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDF--SVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVGT +L+ D EVSCQTGHLI+DS  QT+ DD  D + IT   +DF  + ED L    K   +KEIQFETTEDSYSEH K++KSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEV E MIM  KE+NLE S   DDLI+ESTQ+DGE + VT A+ KE+KGKKLQVG+ +L  D EVSCQTGHLI
Sbjct: 3475 KKLQVGTSKLEGDLEVSCQTGHLIIDSECQTEFDDLSDTVTITLNSKDFGENKEDSL----KEYLEKEIQFETTEDSYSEHVKANKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVEEFMIMERKERNLEESLFFDDLIDESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTGHLI 3660

HSP 10 Score: 60.8474 bits (146), Expect = 3.833e-70
Identity = 29/51 (56.86%), Postives = 41/51 (80.39%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E++E MIM +KE+NLEAS  ++DLI+E+TQYDD+ +  T A  K+VKGKK+
Sbjct: 3427 EVNEIMIMGSKEQNLEASRLIDDLIEESTQYDDEVIIVTNAMQKEVKGKKL 3477

HSP 11 Score: 211.075 bits (536), Expect = 1.309e-69
Identity = 126/188 (67.02%), Postives = 145/188 (77.13%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG PRLD D EVSCQTGHLI+DS  QT+ID+  D + IT   +DFS  +KLF+E K   +KEIQFET E S S+  K+ KSKK+QVGHPRLN++LE ECQSGC+MDDIGVQCE +E MI  + EQNLEAS LIDDLIEE+ QY  E   VT   QKE K KKLQVG P+L+GDLEVSCQTGHLI
Sbjct: 2826 KKLQVGIPRLDVDFEVSCQTGHLIIDSECQTEIDNEIDTLTITMHSKDFS--EKLFKEPKNYLEKEIQFETAEVSDSQLVKTKKSKKIQVGHPRLNKSLEGECQSGCIMDDIGVQCETDEFMIEENNEQNLEASLLIDDLIEENIQYSDESSSVTVLSQKEQKRKKLQVGNPRLDGDLEVSCQTGHLI 3011

HSP 12 Score: 70.8626 bits (172), Expect = 1.309e-69
Identity = 34/51 (66.67%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMI E KE+NLE  LFVEDLI+EATQYD++NL+ TI A  + KGKK+
Sbjct: 2778 EVDEFMIEERKEKNLEERLFVEDLIEEATQYDEENLNVTIGAQIETKGKKL 2828

HSP 13 Score: 201.445 bits (511), Expect = 6.129e-66
Identity = 121/195 (62.05%), Postives = 152/195 (77.95%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEK-KIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            +R  + KKLQVG PRLD D EVSCQTG +I DS  QTD++D+ + ++IT   +DFS  +KLF+E  K  ++KEIQ ET +DS S+  K++KSKKVQVGHPRLNE L+VECQSGC+MDDIGVQCEV+E MI  +KEQNLEAS+ I+DLI+E+TQYD E + +T   Q +  GKKLQVG  K+ GDLEVSCQTGHL+
Sbjct: 2022 LREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLMITMHSKDFS--EKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEENKEQNLEASQFIEDLIDEATQYDDEPVKLTIVSQTKKIGKKLQVGISKMNGDLEVSCQTGHLV 2214

HSP 14 Score: 68.1662 bits (165), Expect = 6.129e-66
Identity = 33/51 (64.71%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMI E KE+NLEASLF+EDLIDEATQYDD+ +   I + ++ KGKK+
Sbjct: 1980 EVDEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKGIINSLREQKGKKL 2030

HSP 15 Score: 197.208 bits (500), Expect = 6.112e-64
Identity = 117/189 (61.90%), Postives = 147/189 (77.78%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVII-VTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG  +++ D EVSCQTGHL++DS +QT +++  DK+  T   + FS E+ L    K  FDKEIQ ETT+DS SEH K++KSKKVQVGHPRLNE L+VECQSG +MDDIGVQCEV+E MI  +KEQNLEAS  I+DLI+E+TQYD E +  + N++ +E KGKKLQVG P+L+GD EVSCQTG +I
Sbjct: 2191 KKLQVGISKMNGDLEVSCQTGHLVIDSESQTQLEEPNDKLTTTTYSKVFS-EEILIGPIKDYFDKEIQCETTDDSISEHNKANKSKKVQVGHPRLNEILDVECQSGYIMDDIGVQCEVDEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKGIINSL-REQKGKKLQVGIPRLDGDFEVSCQTGRII 2377

HSP 16 Score: 65.855 bits (159), Expect = 6.112e-64
Identity = 32/51 (62.75%), Postives = 39/51 (76.47%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMI E KE+NLEAS F+EDLIDEATQYDD+ +  TI +     GKK+
Sbjct: 2143 EVDEFMIEENKEQNLEASQFIEDLIDEATQYDDEPVKLTIVSQTKKIGKKL 2193

HSP 17 Score: 202.216 bits (513), Expect = 2.988e-59
Identity = 124/200 (62.00%), Postives = 150/200 (75.00%), Query Frame = 3 
Query:  123 LLIRT*RE---KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEK-KIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            ++I + RE   KKLQVG PRLD D EVSCQTG +I DS  QTD++D+ + + IT   +DFS  +KLF+E  K  ++KEIQ ET   S SEH K++KSKKVQVGHPRLNE L+VECQSGC+MDDIGVQCEV+E MI   KE+NLE    ++DLIEE+TQYD E + VT   Q E KGKKLQVG P+L+ D EVSCQTGHLI
Sbjct: 2652 VIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDFS--EKLFKEPMKEYYEKEIQCETPNYSISEHNKANKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEERKEKNLEERLFVEDLIEEATQYDEENLNVTIGAQIETKGKKLQVGIPRLDVDFEVSCQTGHLI 2849

HSP 18 Score: 179.104 bits (453), Expect = 5.064e-58
Identity = 106/169 (62.72%), Postives = 130/169 (76.92%), Query Frame = 3 
Query:  204 TGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            T HL++DS +QT +++  DK+  T   + FS E+ L    K  FDKEIQ ETT+DS SEH K++KSKKVQVGHPRLNE L+VECQSGC+MDDIGVQCEV+E MI  +KEQNLEAS  I+DLI+E+TQYD E + V    Q+E KGKKLQVG P+L+GD EVSCQTG +I
Sbjct: 2519 TRHLVIDSESQTQLEEPNDKLTTTTYSKVFS-EEILIGPIKDYFDKEIQCETTDDSISEHNKANKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKLQVGIPRLDGDFEVSCQTGRII 2686

HSP 19 Score: 63.929 bits (154), Expect = 5.064e-58
Identity = 30/44 (68.18%), Postives = 37/44 (84.09%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHK 133
            E+DEFMI E KE+NLEAS F+EDLIDEATQYDD+ +  TI ++K
Sbjct: 2469 EVDEFMIEENKEQNLEASQFIEDLIDEATQYDDEPVKLTIVSNK 2512

HSP 20 Score: 162.54 bits (410), Expect = 3.058e-57
Identity = 95/188 (50.53%), Postives = 130/188 (69.15%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG  +LD D EVSCQTG LIM+S  QT+ ++     + +    D  +     Q+KK   +KE+Q ++ ++S  +  K +KS KVQ+G  R+NE  EVECQSGC+MDD+GVQC++NE  I+ S+E+ +EAS  IDDLI+E+TQYD   + V    QKE KGKKLQVG+P+LE D ++SCQ GHL+
Sbjct: 4602 KKLQVGCSKLDEDLEVSCQTGRLIMNSECQTEFNEQIIITMNSPDFTDLKI-----QQKKDFMEKEMQCDSLDNSLLKENKITKSMKVQMGLARINECREVECQSGCMMDDVGVQCDINESEIIESRERKIEASLFIDDLIDETTQYDDLSLNVRIISQKEYKGKKLQVGSPRLELDSDMSCQVGHLV 4784

HSP 21 Score: 78.1814 bits (191), Expect = 3.058e-57
Identity = 36/51 (70.59%), Postives = 45/51 (88.24%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMIME KERNLEASL +EDLI+E+TQYDD+NL+ TI+  K+ KGKK+
Sbjct: 4554 ELDEFMIMENKERNLEASLLIEDLIEESTQYDDENLNVTISVLKETKGKKL 4604

HSP 22 Score: 192.586 bits (488), Expect = 6.746e-56
Identity = 119/191 (62.30%), Postives = 147/191 (76.96%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVF---DKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVGT RLD+D EVSCQTGHLI +S  QT+ D+  D+I      +DFS E    + K+ VF   +KEIQFE ++D++S   KS+KSKKVQVGH +L+E +EVECQSG +MD+IGVQCE++E MIM +KE+NLEAS LI+DLIEESTQYD E + VT ++ KE KGKKLQVG  KL+ DLEVSCQTG LI
Sbjct: 4439 KKLQVGTSRLDSDLEVSCQTGHLITESECQTEFDEKVDEITFLLNAQDFSEE----KPKRSVFEFVNKEIQFEASDDTHSISDKSNKSKKVQVGHAKLHEGVEVECQSGYMMDEIGVQCELDEFMIMENKERNLEASLLIEDLIEESTQYDDENLNVTISVLKETKGKKLQVGCSKLDEDLEVSCQTGRLI 4625

HSP 23 Score: 189.889 bits (481), Expect = 6.209e-55
Identity = 115/189 (60.85%), Postives = 145/189 (76.72%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVF-DKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQ+G PRLD + EVSCQTGHLI+DS  QTD+D+  D  IIT    DF+  ++L +E    F +KE+Q+   +D    H +++KSKKVQVGHPRLNE+L+VECQ+GCLMDDI VQC++ E MIM +K +NLEA+  I+DLIEESTQYD   + +T A+ KEVKGKKLQVGT +L+ DLEVSCQTGHLI
Sbjct: 4276 KKLQIGFPRLDGELEVSCQTGHLILDSHCQTDLDELTDTFIITNHLPDFT--ERLNREFLKEFAEKEVQYGDFDDYSLIHDEANKSKKVQVGHPRLNESLDVECQTGCLMDDIEVQCKLEEFMIMENKVRNLEANLFIEDLIEESTQYDEGNLNITTAIHKEVKGKKLQVGTSRLDSDLEVSCQTGHLI 4462

HSP 24 Score: 161.384 bits (407), Expect = 5.335e-54
Identity = 100/162 (61.73%), Postives = 128/162 (79.01%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEK-KIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVT 611
            +R  + KKLQVG PRLD D EVSCQTG +I DS  QTD++D+ + ++IT   +DFS  +KLF+E  K  ++KEIQ ET +DS S+  K++KSKKVQVGHPRLNE L+VECQSGC+MDDIGVQCEV+E MI  +KEQNLEAS+ I+DLI+E+TQYD E + +T
Sbjct: 2348 LREQKGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLMITMHSKDFS--EKLFKEPMKEYYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEENKEQNLEASQFIEDLIDEATQYDDEPVKLT 2507

HSP 25 Score: 68.1662 bits (165), Expect = 5.335e-54
Identity = 33/51 (64.71%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMI E KE+NLEASLF+EDLIDEATQYDD+ +   I + ++ KGKK+
Sbjct: 2306 EVDEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKGIINSLREQKGKKL 2356

HSP 26 Score: 148.288 bits (373), Expect = 1.037e-47
Identity = 90/188 (47.87%), Postives = 120/188 (63.83%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG+PRL+ D ++SCQ GHL+ DS  QT+  D+       +   +   E  L    K   +KEIQFE  ED  S       SK VQVG  RL E  +VECQ+ C+ DD+GVQ EV+EIMIM  +E + E   ++D +I+E+TQY G+ IIV  ++QK++K KKLQVG  +L+ D EVSCQTG+ I
Sbjct: 4761 KKLQVGSPRLELDSDMSCQVGHLVFDSECQTESFDSISITKYRSDFSEVPTERHL----KDFVEKEIQFELLEDCSSTINNVKISKNVQVGQARLAEGRDVECQAVCMKDDVGVQNEVDEIMIMTERENHFEERFVLDLMIDETTQY-GDEIIVYCSLQKDLKNKKLQVGNSRLDIDSEVSCQTGNFI 4943

HSP 27 Score: 60.077 bits (144), Expect = 1.037e-47
Identity = 28/51 (54.90%), Postives = 41/51 (80.39%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            +I+E  I+E++ER +EASLF++DLIDE TQYDD +L+  I + K+ KGKK+
Sbjct: 4713 DINESEIIESRERKIEASLFIDDLIDETTQYDDLSLNVRIISQKEYKGKKL 4763

HSP 28 Score: 167.933 bits (424), Expect = 1.127e-47
Identity = 103/190 (54.21%), Postives = 128/190 (67.37%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVED--KLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            K +Q G  +L  D E SCQTGHLI+DS  QT+ D+  + I ++   +DFS E   K+    K   DKEIQF+   DS  +       K +QVGH RLN+  EVECQSGC+MDD+ VQ EV+E MIM  KE+NLE S   DDLI+ESTQ+DGE + VT A+ KE+KGKKLQVG+ +L  D EVSCQTGHLI
Sbjct: 3151 KIIQAGFSKLGFDLEASCQTGHLIIDSECQTEFDNNNEIISLSLNYQDFSEESSGKII---KYFDDKEIQFDALCDSIPKLHPIKSKK-IQVGHTRLNDYREVECQSGCIMDDVEVQTEVDEFMIMERKERNLEESLFFDDLIDESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTGHLI 3336

HSP 29 Score: 152.91 bits (385), Expect = 1.127e-47
Identity = 103/188 (54.79%), Postives = 132/188 (70.21%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVGTP+L+ D EVSCQTGHLI+DS  QT+ DD  D +IIT   +D+  +++     K   +KE+QFE  E+SY+   K+  S KVQVG PRL+EN EVECQSG +MDD+ VQ EV E+M++ +KE++LE   L DDLI ES QY+             VKGKKLQ+G P+L+G+LEVSCQTGHLI
Sbjct: 4125 KKLQVGTPKLEGDLEVSCQTGHLIIDSECQTEFDDLTDTVIITLNSKDYGEKNE--NPLKEYLEKEMQFEPIEESYTRLVKTYASMKVQVGFPRLDENREVECQSGSIMDDVEVQTEVVEVMMLDNKERSLEFGFLFDDLINESIQYNV-----------AVKGKKLQIGFPRLDGELEVSCQTGHLI 4299

HSP 30 Score: 55.8398 bits (133), Expect = 1.127e-47
Identity = 27/51 (52.94%), Postives = 38/51 (74.51%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E++E MIM  KE+NLE S  ++DLI+E+TQYD + +  T A  K+VKGKK+
Sbjct: 4077 EVNEIMIMGNKEQNLEVSRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKL 4127

HSP 31 Score: 40.4318 bits (93), Expect = 1.127e-47
Identity = 21/53 (39.62%), Postives = 35/53 (66.04%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKIAS 160
            E D+F+I++ KE +LE SL  ++LI E  QYDD+++   ++   + KGK I +
Sbjct: 3103 ETDKFIIIDFKEDSLETSLRTDNLIHEEIQYDDEDVKMRLSPQAEHKGKIIQA 3155

HSP 32 Score: 163.696 bits (413), Expect = 6.596e-46
Identity = 108/200 (54.00%), Postives = 139/200 (69.50%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKL----FQE---KKIVFDKEIQFETTEDSYSEHG----XXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVII-VTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVGT RL+ D + SCQTGH++ D  +QT+I++T   I+         +E KL    FQE   K  +  +EI+ +T  D +          +KSKKVQVGHPRLNE L+VECQSGC+MDDIGVQCEV+E MI  +KEQNLEAS  I+DLI+E+TQYD E +  + N++ +E KGKKLQVG P+L+GD EVSCQTG +I
Sbjct: 1864 KKLQVGTSRLEYDFDASCQTGHIVKDDCDQTEIEETVGSIL---------IESKLALNEFQEMTSKDSISLREIEIQT--DDFPGFSVNIINMNKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQCEVDEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKGIINSL-REQKGKKLQVGIPRLDGDFEVSCQTGRII 2051

HSP 33 Score: 150.599 bits (379), Expect = 2.716e-41
Identity = 97/190 (51.05%), Postives = 122/190 (64.21%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            + KKLQVG PRLD D EVSCQTGHLI+DS  QT+ D   D   IT   +DF+   + +    +  +KEIQFE  +DS+    K  KSKK+QVG  +L+E  E ECQ+GCLMDD GVQ E ++ +I+  KE +LE S   D+LI E  QYD E + +  + Q E KGK +Q G  KL  DLE SCQTGHLI
Sbjct: 2986 KRKKLQVGNPRLDGDLEVSCQTGHLIIDSQCQTEFDWLPDTSAITLVSKDFNQVARKYSLTDLA-EKEIQFELIDDSFLRDEKCKKSKKIQVGFSKLSECSEAECQTGCLMDDTGVQYETDKFIIIDFKEDSLETSLRTDNLIHEEIQYDDEDVKMRLSPQAEHKGKIIQAGFSKLGFDLEASCQTGHLI 3174

HSP 34 Score: 129.798 bits (325), Expect = 4.407e-34
Identity = 98/205 (47.80%), Postives = 130/205 (63.41%), Query Frame = 3 
Query:   96 MIRTCILQLLLIRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            +I  C LQ  L    + KKLQVG  RLD D EVSCQTG+ IMDS  QT+++D+ D I+IT Q ++F  E K    + +  +KEIQ E      S + K S S+ +QVG  +LNE  EVECQS  ++ ++GVQ E  E MI+    +N++   LI DLIEE+ QY+ +V    N   K++KGKKLQVG P L+ D EVSCQTGHL+
Sbjct: 4907 IIVYCSLQKDL----KNKKLQVGNSRLDIDSEVSCQTGNFIMDSECQTEVEDSTDMILITHQFQNFEEERKPHVREFV--EKEIQSEF----LSRYNKPSSSQVIQVGLSKLNEYKEVECQSEFIVFEMGVQTEAKESMIIVGTGRNVDQDLLIYDLIEETVQYNEDV----NEAFKKLKGKKLQVGNPYLDSDSEVSCQTGHLL 5097

HSP 35 Score: 115.161 bits (287), Expect = 5.893e-29
Identity = 90/190 (47.37%), Postives = 123/190 (64.74%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSK--EQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQVG P LD+D EVSCQTGHL+++S  QT++ D  D II T   +DFS E+ + +  K   +KEIQFET + S S++     SK+VQVG  RL E+ EV CQS  LM+DIGV+ E  E  IM +K  ++NL  S    ++IEE  QY  +        +K+ KGKKLQ G+ +L G++++ CQTGHL+
Sbjct: 5074 KKLQVGNPYLDSDSEVSCQTGHLLIESECQTELGDEFDTIIKTEHTQDFS-EELMERPMKEFVEKEIQFETAKSSLSKYNTLENSKEVQVGQSRLVESREVGCQSEYLMNDIGVETEERECWIMEAKYNQENLSVSY---EMIEEIIQYFSDDFTDV-PKKKDQKGKKLQAGSSRLLGEMDLLCQTGHLM 5258

HSP 36 Score: 113.235 bits (282), Expect = 2.148e-28
Identity = 72/190 (37.89%), Postives = 110/190 (57.89%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            +EK  QVG PR++ D + SCQ GHL+ +   QTD +            +D      + +      +KE+Q     D  SE  + SK   VQVGHPRLN+NL+VECQSG L++++ +Q E++E +I  + + N   ++  +++IEE  QY+ E +     ++   KGKKLQ G+ +LE D + SCQTGHL+
Sbjct: 1543 QEKNFQVGNPRIEIDYDTSCQAGHLVRELEIQTDQEPFDGFETQITMVKDQLESQNIVRTTTEFIEKEVQ----PDFVSEKSRKSKK--VQVGHPRLNDNLDVECQSGTLLNEMEIQTELDEFIIEQTTQINENLTQSSEEMIEELVQYESENLRPI-TLKTNTKGKKLQTGSSRLENDFDSSCQTGHLV 1725

HSP 37 Score: 97.4413 bits (241), Expect = 6.206e-23
Identity = 70/189 (37.04%), Postives = 113/189 (59.79%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVF-DKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            K +Q G+P+L  + + SCQ G++  ++  QTD + + ++++IT    +   ++ L + KK  + +KEIQ   T    S  GK      +QVGHP+LNE +EVECQS   +++I VQ E++E +I   + +N  A+  ++++IEES QY  + I  T    KE + K  QVG P++E D + SCQ GHL+
Sbjct: 1386 KNVQSGSPKLFMELDESCQIGYVKHEAECQTDNEFSDEELVITEVQAELQSQNVLMESKKKEYTEKEIQPILTFYKASNKGKK-----IQVGHPKLNEFVEVECQSEISIEEIAVQTEIDEFVIEEIRNKNELAALFVEEMIEESVQYMADDIF-TIQYPKETQEKNFQVGNPRIEIDYDTSCQAGHLV 1568

HSP 38 Score: 90.5077 bits (223), Expect = 1.850e-20
Identity = 74/199 (37.19%), Postives = 115/199 (57.79%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEK---KIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQ--------YDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            KKLQ G+ RL+ D + SCQTGHL+ +S  QT+ D+   KI    +    + E   FQ +   K   D+E+Q +  +D      +++K KKVQVG+PRLN  +  ECQS  ++ +  +Q E +E++     ++NLEA  +  ++I E+TQ        +DG+++ +         GKKLQVGT +LE D + SCQTGH++
Sbjct: 1702 KKLQTGSSRLENDFDSSCQTGHLVTESEVQTENDEFGGKIDKEIR---LANEQTQFQNELLFKEYVDQEVQLDI-DDFTGPIPRNTKCKKVQVGYPRLNLQIHAECQSEIVLQNEDIQTENDELLHEVVIDRNLEAFLVNKEMINEATQCNFDLMQPHDGKLMGI---------GKKLQVGTSRLEYDFDASCQTGHIV 1887

HSP 39 Score: 75.485 bits (184), Expect = 2.098e-15
Identity = 38/67 (56.72%), Postives = 52/67 (77.61%), Query Frame = 2 
Query:    5 IDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI---ASR---NTEIRCRS 187
            ++EFMIME K RNLEA+LF+EDLI+E+TQYD+ NL+ T A HK+VKGKK+    SR   + E+ C++
Sbjct: 4392 LEEFMIMENKVRNLEANLFIEDLIEESTQYDEGNLNITTAIHKEVKGKKLQVGTSRLDSDLEVSCQT 4458

HSP 40 Score: 70.4774 bits (171), Expect = 1.273e-13
Identity = 33/51 (64.71%), Postives = 42/51 (82.35%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKI 154
            E+DEFMI E KE+NLEASLF+EDLIDEATQYDD+ +   I + ++ KGKK+
Sbjct: 2615 EVDEFMIEENKEQNLEASLFIEDLIDEATQYDDEPVKVIINSQREQKGKKL 2665

HSP 41 Score: 68.5514 bits (166), Expect = 5.508e-13
Identity = 60/198 (30.30%), Postives = 89/198 (44.95%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVII--VTNAMQKE--VKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            ++T   K+ Q+G  RLD D E SCQTGH++ D  +QT+               D++             + +IQ  T  ++        K KKVQVG+P+LN + E   Q G  ++D  +Q +                   I++ I   T  DGE+    V    Q    +K KKLQ G+ +L  D E SCQTGH++
Sbjct: 1123 VKTTENKETQIGCSRLDGDFETSCQTGHIVKDFESQTE--------------NDYT-------------EHQIQLVTMANNLQAVKSQMKGKKVQVGYPQLNMSFEFGSQFGDDLNDATIQTDS------------------IEEYINPMTLTDGEIQWNDVEEGSQSSSGLKSKKLQAGSSRLNADYEASCQTGHIV 1275

HSP 42 Score: 67.3958 bits (163), Expect = 1.177e-12
Identity = 67/203 (33.00%), Postives = 109/203 (53.69%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYS-EHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEV-NEI----MIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKG---KKLQVGTPKLEGDLEVSCQTGHLI 710
            ++  + KK+QVG PRL+ + EV CQ+G L+ D G Q      + +  +  + ++ ++E+ LF +  I  D+  QF+    + +    K  K KK+QVG  RL ++ EV CQ+G L+ +   Q E+ NEI    + M SK+ +    R      E+  Q++       N+  + VK    KK+QVG P+L  +LEV CQ+G L+
Sbjct: 3552 VKANKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQ-----CEVEEFMIMERKERNLEESLFFDDLI--DESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTGHLIMNSECQTEIDNEIDTLTITMHSKDYSERLFREPIKYFEKEIQFE----TTENSYSEHVKASKSKKVQVGHPRLNENLEVECQSGCLM 3743

HSP 43 Score: 67.3958 bits (163), Expect = 1.269e-12
Identity = 64/201 (31.84%), Postives = 103/201 (51.24%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXX-XXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEE-STQYDGEVIIVT-----NAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            ++  + KK+QVG PRL+ + EV CQ+G L+ D G Q      +    +  + ++ ++E  LF E  I  D+  Q++     ++         KK+QVG PRL+ + EV CQ+G L+ D G Q ++++I              + D L +E    +D E+   T     +   K  K KK+QVG P+L  +LEV CQ+G L+
Sbjct: 3876 VKANKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQ-----CEVDEFMIMETKERNLEASLFVEDLI--DEATQYDDKNLHFTIAAHKDVKGKKLQVGTPRLDADPEVSCQTGHLIMDSGDQTDIDDIKDKIIITAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGKSSKSKKVQVGHPRLNENLEVECQSGCLM 4069

HSP 44 Score: 65.855 bits (159), Expect = 4.449e-12
Identity = 63/202 (31.19%), Postives = 99/202 (49.01%), Query Frame = 3 
Query:  126 LIRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEI---MIMGSKEQNLEASRL----IDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            L++T    K+QVG PRLD + EV CQ+G  IMD             +    +  +F     LF +   + ++ IQ+            + K KK+Q+G PRL+  LEV CQ+G L+ D   Q +++E+    I+ +   +    RL    + +  E+  QY G+    +    +  K KK+QVG P+L   L+V CQTG L+
Sbjct: 4201 LVKTYASMKVQVGFPRLDENREVECQSGS-IMDDVEVQTEVVEVMMLDNKERSLEFGF---LFDD---LINESIQY----------NVAVKGKKLQIGFPRLDGELEVSCQTGHLILDSHCQTDLDELTDTFIITNHLPDF-TERLNREFLKEFAEKEVQY-GDFDDYSLIHDEANKSKKVQVGHPRLNESLDVECQTGCLM 4383

HSP 45 Score: 65.0846 bits (157), Expect = 7.724e-12
Identity = 68/210 (32.38%), Postives = 108/210 (51.43%), Query Frame = 3 
Query:  126 LIRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQ--EKKIVFD----KEIQFETTEDSYSEHGXX-XXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEI-----MIMGSKEQNLEASRL-IDDLIEESTQYDGEVIIVTNAMQKE--VKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            L++T + KK+QVG PRL+   E  CQ+G ++ D G Q              +  +F +E+   Q  E  ++ D    + IQ+     S +   +   K KK+QVG+PRL+ +LEV CQ+G L+ D   Q E + +     + + SK+ N  A +  + DL E+  Q++   +I  + ++ E   K KK+QVG  KL    E  CQTG L+
Sbjct: 2902 LVKTKKSKKIQVGHPRLNKSLEGECQSGCIMDDIGVQ-------------CETDEFMIEENNEQNLEASLLIDDLIEENIQYSDESSSVTVLSQKEQKRKKLQVGNPRLDGDLEVSCQTGHLIIDSQCQTEFDWLPDTSAITLVSKDFNQVARKYSLTDLAEKEIQFE---LIDDSFLRDEKCKKSKKIQVGFSKLSECSEAECQTGCLM 3095

HSP 46 Score: 63.929 bits (154), Expect = 1.791e-11
Identity = 60/199 (30.15%), Postives = 100/199 (50.25%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEI-----MIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            ++  + KK+QVG PRL+ + EV CQ+G L+ D G Q ++++               + D L +E    +D E+   T        G     KK+QVG  +L  +LEV CQ+G L+ D   Q E +++     + + SK+        + + +E+  Q++      +  + K  K KK+QVG P+L  +LEV CQ+G L+
Sbjct: 3390 VKASKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEE-STQYDDEVIIVTNAMQKEVKG-----KKLQVGTSKLEGDLEVSCQTGHLIIDSECQTEFDDLSDTVTITLNSKDFGENKEDSLKEYLEKEIQFETTEDSYSEHV-KANKSKKVQVGHPRLNENLEVECQSGCLM 3581

HSP 47 Score: 63.929 bits (154), Expect = 1.791e-11
Identity = 60/199 (30.15%), Postives = 100/199 (50.25%), Query Frame = 3 
Query:  129 IRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEI-----MIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            ++  + KK+QVG PRL+ + EV CQ+G L+ D G Q ++++               + D L +E    +D E+   T        G     KK+QVG  +L  +LEV CQ+G L+ D   Q E +++     + + SK+        + + +E+  Q++      +  + K  K KK+QVG P+L  +LEV CQ+G L+
Sbjct: 3714 VKASKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEE-STQYDDEVIIVTNAMQKEVKG-----KKLQVGTSKLEGDLEVSCQTGHLIIDSECQTEFDDLSDTVTITLNSKDFGENKEDSLKEYLEKEIQFETTEDSYSEHV-KANKSKKVQVGHPRLNENLEVECQSGCLM 3905

HSP 48 Score: 63.929 bits (154), Expect = 2.003e-11
Identity = 63/196 (32.14%), Postives = 103/196 (52.55%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKI----VFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEV-NEI----MIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTG 701
            + KK+QVG PRL+   +V CQ+G ++ D G Q      +    +  + ++ ++E++LF E  I     +D+E    T        G     KK+QVG PRL+ + EV CQ+G L+ D   Q E+ NEI    + M SK+ + +  +   + +E+  Q++    +  + + K  K KK+QVG P+L   LE  CQ+G
Sbjct: 2745 KSKKVQVGHPRLNEILDVECQSGCIMDDIGVQ-----CEVDEFMIEERKEKNLEERLFVEDLIEEATQYDEENLNVTIGAQIETKG-----KKLQVGIPRLDVDFEVSCQTGHLIIDSECQTEIDNEIDTLTITMHSKDFSEKLFKEPKNYLEKEIQFET-AEVSDSQLVKTKKSKKIQVGHPRLNKSLEGECQSG 2929

HSP 49 Score: 63.1586 bits (152), Expect = 3.378e-11
Identity = 58/195 (29.74%), Postives = 98/195 (50.26%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            + KK+QVG PRL+ + EV CQ+G L+ D G Q ++++               + D L +E    +D E+   T        G     KK+QVG P+L  +LEV CQ+G L+ D   Q E ++     I+ + SK+   +    + + +E+  Q++  +      + K     K+QVG P+L+ + EV CQ+G ++
Sbjct: 4044 KSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGNKEQNLEVSRLIDDLIEE-STQYDGEVIIVTNAMQKEVKG-----KKLQVGTPKLEGDLEVSCQTGHLIIDSECQTEFDDLTDTVIITLNSKDYGEKNENPLKEYLEKEMQFEP-IEESYTRLVKTYASMKVQVGFPRLDENREVECQSGSIM 4231

HSP 50 Score: 62.003 bits (149), Expect = 8.580e-11
Identity = 56/191 (29.32%), Postives = 101/191 (52.88%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVK-GKKLQVGTPKLEGDLEVSCQTGHLI 710
            + K++Q G  +L  + E S QT + ++++  QT+ +      +      ++ +   L QE K   D EIQ +   +   + GK      VQ+G  +L E  E  CQ G ++ +  +Q +     ++ + E+   +S +  +L +ESTQ+   +I + N ++  +K  KKLQ+G  +LEG+LE SCQTGH++
Sbjct:  859 KNKRIQFGKSKLFGEGEFSSQTNYHLIEANVQTNNEVEDYATLSENGMLEYEI---LTQEPKQTIDVEIQSDL--EIVKQKGKK-----VQIGSSKLLETCESSCQIGTVLHNSELQTDDKLKFVIQANEK---SSPIGLELKDESTQWG--IIPIDNEIEHGMKKNKKLQIGNSRLEGNLESSCQTGHVV 1034

HSP 51 Score: 60.4622 bits (145), Expect = 2.798e-10
Identity = 56/191 (29.32%), Postives = 96/191 (50.26%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEV--KGKKLQVGTPKLEGDLEVSCQTGHL 707
            + KKLQ G+ RL+AD E SCQTGH++    N+ +          +  P+++S             +KE Q E+           +K KK+QVG+P +N+  E E Q    + +  +Q +      +GS+++ +       +LI+E  QY G    + + +  E+  +GK +Q G+PKL  +L+ SCQ G++
Sbjct: 1250 KSKKLQAGSSRLNADYEASCQTGHIV----NEIETQTEFFYKTESILPKEYS-------------NKETQPES-------EMAITKGKKIQVGYPHINKISEKEIQIEIDITESSIQTDQ-----IGSEKEMINQRSFGLELIDEELQYYGS---LQDRLGNELFYEGKNVQSGSPKLFMELDESCQIGYV 1408

HSP 52 Score: 60.4622 bits (145), Expect = 2.877e-10
Identity = 55/190 (28.95%), Postives = 85/190 (44.74%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTGHLI 710
            + KKLQ+G  RL+ + E SCQTGH++ +   Q                     E+ LF E   +  KEIQ +       +     K KKVQVG+P+  + ++VECQ         VQ    E     ++ + +  +R       E    + E  +      K  + K+ Q+G  +L+GD E SCQTGH++
Sbjct: 1009 KNKKLQIGNSRLEGNLESSCQTGHVVDEFHTQ--------------------TEELLFMEPMGLLSKEIQSDIESADKYQIRLEMKGKKVQVGYPQTYDRIDVECQ---------VQSSFFE-----AETETISLAR------NEFEMNEREWTV------KTTENKETQIGCSRLDGDFETSCQTGHIV 1152

HSP 53 Score: 58.9214 bits (141), Expect = 7.701e-10
Identity = 65/199 (32.66%), Postives = 104/199 (52.26%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYS-EHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEV-NEI----MIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKG---KKLQVGTPKLEGDLEVSCQTGHLI 710
            + KK+QVG  RL+   EV CQ+G ++ D   Q     T+    +  + ++ ++E+ LF +  I  D+  QF+    + +    K  K KK+QVG  RL ++ EV CQ+G L+ +   Q E+ NEI    + M SK+ +    R      E+  Q++       N+  + VK    KK+QVG P+L  +LEV CQ+G L+
Sbjct: 3232 KSKKIQVGHTRLNDYREVECQSGCIMDDVEVQ-----TEVDEFMIMERKERNLEESLFFDDLI--DESTQFDGENLNVTVAVPKEIKGKKLQVGSARLYDDFEVSCQTGHLIMNSECQTEIDNEIDTLTITMHSKDYSERLFREPIKYFEKEIQFE----TTENSYSEHVKASKSKKVQVGHPRLNENLEVECQSGCLM 3419

HSP 54 Score: 58.9214 bits (141), Expect = 8.218e-10
Identity = 63/202 (31.19%), Postives = 106/202 (52.48%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTE-----DSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGSKEQNLEASRLIDDLIEESTQYDGEVII-----VTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTG 701
            + KK+QVG PRL+   +V CQ+G+++ D G Q      +    +  + ++ ++E  LF E  I  D+  Q++        +S  E     K KK+QVG PRL+ + EV CQ+G ++ D   Q ++N+     ++ M SK+    + +L  + ++E   Y+ E+        T+   K  K KK+QVG P+L   L+V CQ+G
Sbjct: 2273 KSKKVQVGHPRLNEILDVECQSGYIMDDIGVQ-----CEVDEFMIEENKEQNLEASLFIEDLI--DEATQYDDEPVKGIINSLREQ----KGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLMITMHSKDF---SEKLFKEPMKE--YYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 2458

HSP 55 Score: 56.9954 bits (136), Expect = 4.160e-9
Identity = 64/207 (30.92%), Postives = 107/207 (51.69%), Query Frame = 3 
Query:  126 LIRT*REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTE-----DSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGSKEQNLEASRLIDDLIEESTQYDGEVII-----VTNAMQKEVKGKKLQVGTPKLEGDLEVSCQTG 701
            +I   + KK+QVG PRL+   +V CQ+G ++ D G Q      +    +  + ++ ++E  LF E  I  D+  Q++        +S  E     K KK+QVG PRL+ + EV CQ+G ++ D   Q ++N+     ++ M SK+    + +L  + ++E   Y+ E+        T+   K  K KK+QVG P+L   L+V CQ+G
Sbjct: 1942 IINMNKSKKVQVGHPRLNEILDVECQSGCIMDDIGVQ-----CEVDEFMIEENKEQNLEASLFIEDLI--DEATQYDDEPVKGIINSLREQ----KGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLMITMHSKDF---SEKLFKEPMKE--YYEKEIQCETPDDSTSDQIKANKSKKVQVGHPRLNEILDVECQSG 2132

HSP 56 Score: 56.6102 bits (135), Expect = 5.340e-9
Identity = 67/187 (35.83%), Postives = 95/187 (50.80%), Query Frame = 3 
Query:  147 KKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMD-DIGVQCEVNEIM-IMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVK-GKKLQVGTPKLEGDLEVSCQT 698
            KKLQVG  RL  D +   QT   + DS N  D+   + +         FS  DK   E + +FD+E++F TT + +  H        VQVG+PRLN  ++ E Q    +D D     E +EI   + SKEQ        D+LI+E+ QY   + + ++     VK  KKLQVG+ +LE D + SCQT
Sbjct: 5542 KKLQVGNSRLLDDSDEFTQTE--LNDSRNIEDLCIQQVQSFQNTNTISFS--DK---EVEPLFDEEVEFPTT-NVFKSHS-------VQVGYPRLNACVDFEGQYSFDIDFDGSDHFESSEIEDRVFSKEQ-------FDELIDETVQYS--IGMQSSTYPSSVKVNKKLQVGSSRLEVDHDQSCQT 5704

HSP 57 Score: 56.225 bits (134), Expect = 7.519e-9
Identity = 63/202 (31.19%), Postives = 104/202 (51.49%), Query Frame = 3 
Query:  141 REKKLQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTE-----DSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNE-----IMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVT-----NAMQKEVKGKKLQVGTPKLEGDLEVSCQTG 701
            + KK+QVG PRL+   +V CQ+G ++ D G Q      +    +  + ++ ++E  LF E  I  D+  Q++        +S  E     K KK+QVG PRL+ + EV CQ+G ++ D   Q ++N+      + M SK+    + +L  + ++E   Y+ E+   T     +   K  K KK+QVG P+L   L+V CQ+G
Sbjct: 2582 KSKKVQVGHPRLNEILDVECQSGCIMDDIGVQ-----CEVDEFMIEENKEQNLEASLFIEDLI--DEATQYDDEPVKVIINSQREQ----KGKKLQVGIPRLDGDFEVSCQTGRIIKDSECQTDLNDSVETLTITMHSKDF---SEKLFKEPMKE--YYEKEIQCETPNYSISEHNKANKSKKVQVGHPRLNEILDVECQSG 2767

HSP 58 Score: 55.4546 bits (132), Expect = 1.309e-8
Identity = 32/97 (32.99%), Postives = 60/97 (61.86%), Query Frame = 3 
Query:  408 VQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQT 698
            VQ+G  R+N    ++ Q+    +D+GVQ  ++E +I+ + +QN+ +  + +DLI E  Q++   ++  ++++K+ KGKKLQVG  +L  D +   QT
Sbjct: 5467 VQIGPSRINLCRTIDSQTQFHGEDVGVQGALDEALIIETTQQNVHSKLIFEDLIVEEIQFN--CLLPRSSIKKDSKGKKLQVGNSRLLDDSDEFTQT 5561

HSP 59 Score: 54.6842 bits (130), Expect = 2.154e-8
Identity = 29/68 (42.65%), Postives = 42/68 (61.76%), Query Frame = 2 
Query:    2 EIDEFMIMETKERNLEASLFVEDLIDEATQYDDKNLHFTIAAHKDVKGKKIASRNT------EIRCRS 187
            E DEFMI E  E+NLEASL ++DLI+E  QY D++   T+ + K+ K KK+   N       E+ C++
Sbjct: 2940 ETDEFMIEENNEQNLEASLLIDDLIEENIQYSDESSSVTVLSQKEQKRKKLQVGNPRLDGDLEVSCQT 3007

HSP 60 Score: 53.5286 bits (127), Expect = 5.122e-8
Identity = 54/184 (29.35%), Postives = 98/184 (53.26%), Query Frame = 3 
Query:  153 LQVGTPRLDADPEVSCQTGHLIMDSGNQXXXXXXXXXXXXXAQPRDFSVEDKLFQEKKIVFDKEIQFETTEDSYSEHGXXXXXXXVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIMGSKEQNLEASRLIDDLIEESTQY--DGEVIIVTNAMQKEVKGKKLQVGTPKLEGDLEVSCQT 698
            LQ G+ RL  + ++ CQTGHL++D+  QT+   T D +       +    D+ F  K +  ++E Q++  E++      S +++ +QVG P+L+++ E+ CQS     D  VQ E     ++ ++E  L+     + +IEE+ QY  DG   I   +M+   K + +Q G  +++ D++ S QT
Sbjct: 5237 LQAGSSRLLGEMDLLCQTGHLMLDNECQTNKMTTNDIMENFQNELN---ADESFPTKSLS-NQESQYDLCEETV----LSCQNQALQVGFPKLHQSQEISCQSTVETVDAVVQSE----SVLTNEEYQLKKQLCQEVMIEETVQYVPDGFGSI---SMKPNTKDRAIQNGNSRIQKDIDASYQT 5405
The following BLAST results are available for this feature:
BLAST of SMED30014416 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014416 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000023005.14.816e-11797.87SMESG000023005.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014416 ID=SMED30014416|Name=SMED30014416|organism=Schmidtea mediterranea sexual|type=transcript|length=710bp
TGAAATAGATGAATTCATGATCATGGAAACTAAAGAGCGAAATCTTGAAG
CGAGTTTATTTGTTGAAGATCTGATTGATGAAGCAACTCAATACGATGAT
AAGAACTTGCATTTTACAATTGCTGCTCATAAGGACGTAAAGGGAAAAAA
AATTGCAAGTAGGAACACCGAGATTAGATGCAGATCCTGAGGTTTCCTGC
CAAACGGGACATTTGATCATGGATTCAGGTAACCAAACAGATATTGATGA
TACAAAAGATAAAATAATTATTACTGCACAACCTCGTGATTTTTCTGTGG
AAGATAAACTATTTCAAGAGAAAAAAATTGTTTTTGACAAAGAAATACAA
TTTGAAACAACTGAAGATTCTTATTCTGAACATGGTAAATCAAGTAAATC
AAAGAAAGTCCAAGTCGGTCATCCAAGATTGAATGAAAATTTAGAAGTGG
AATGCCAATCAGGATGCCTAATGGATGACATCGGGGTTCAATGCGAAGTA
AATGAAATTATGATCATGGGAAGTAAAGAACAAAATCTTGAAGCGAGTCG
TTTAATTGACGATCTGATTGAAGAATCAACTCAATATGATGGTGAAGTTA
TCATTGTTACAAATGCTATGCAGAAAGAAGTCAAAGGAAAGAAATTACAA
GTGGGAACCCCAAAATTAGAAGGAGATTTGGAAGTATCATGTCAGACAGG
ACATTTAATA
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protein sequence of SMED30014416-orf-1

>SMED30014416-orf-1 ID=SMED30014416-orf-1|Name=SMED30014416-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
MDSGNQTDIDDTKDKIIITAQPRDFSVEDKLFQEKKIVFDKEIQFETTED
SYSEHGKSSKSKKVQVGHPRLNENLEVECQSGCLMDDIGVQCEVNEIMIM
GSKEQNLEASRLIDDLIEESTQYDGEVIIVTNAMQKEVKGKKLQVGTPKL
EGDLEVSCQTGHLI
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte