SMED30014371
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014371 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Alignments
SMED30014371 aligns in the following genomic locations:
Homology
BLAST of SMED30014371 vs. TrEMBL
Match: A0A4S2LMN0 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007772 PE=4 SV=1) HSP 1 Score: 173.326 bits (438), Expect = 1.055e-49 Identity = 83/195 (42.56%), Postives = 125/195 (64.10%), Query Frame = 3 Query: 120 FLVYSSIPSAFSSTYSPEFTKQVQEYPNVVTDLKKCVP-QGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFC--TSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKA 695 L++ S+ + Y EFT QVQ YPN++ + +C+P +G Y VCDPD LL + IN+LN +++ +N + C P P P IA+ALVN++K + + +L+ AS+FTY L+++W + S C SDK+IIFYSK DG +YT+AG L+ KLPS++IID AV ++ FS G A GL ++++RY +A Sbjct: 14 LLLFISLFGFTLAEYPHEFTAQVQSYPNIMRESAQCLPLKGLYDHDQTFVCDPDRLLTVDQINHLNSMLKEFRNLRSRDESMCSPHSPHPVIALALVNKLKVGNENPDRLLSYASIFTYYLFNEWNLPSTCHTGSDKVIIFYSKDDGVIYTFAGNLLERKLPSQRIIDFAVEARIHFSNGIAAGLGFLIQRYREA 208
BLAST of SMED30014371 vs. TrEMBL
Match: A0A5J4NE80 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0006470 PE=4 SV=1) HSP 1 Score: 167.933 bits (424), Expect = 9.403e-48 Identity = 81/181 (44.75%), Postives = 117/181 (64.64%), Query Frame = 3 Query: 162 YSPEFTKQVQEYPNVVTDLKKCVP-QGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFC--TSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKA 695 Y EFT QVQ YPN++ + C+P +GNY VCD D LL + IN LN+ +Q+ +N +S C P P P IAIALVN++K + + +L+ AS+F Y L+++W + S C SDK+IIFYSK DG +YT+AG L+ KLPS ++IDIAV ++ +FS G GL Y++++Y + Sbjct: 26 YPLEFTAQVQSYPNIMRAKEDCLPLKGNYEHAQTYVCDVDRLLTVNQINRLNELLQEFRNLKNRDSSACSPANPNPVIAIALVNKLKVGNENPDRLLSYASIFAYYLFNEWNLPSTCHTGSDKMIIFYSKDDGMIYTFAGNLLERKLPSSRLIDIAVQARLYFSNGIYEGLTYLIQKYRQV 206
BLAST of SMED30014371 vs. TrEMBL
Match: A0A074ZXD3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_02101 PE=4 SV=1) HSP 1 Score: 172.17 bits (435), Expect = 1.049e-47 Identity = 84/192 (43.75%), Postives = 120/192 (62.50%), Query Frame = 3 Query: 162 YSPEFTKQVQEYPNVVTDLKKCVP-QGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFCT--SDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKAFLGRDKGVLGN 728 Y EFT QVQ YPN++ + +C+P +G Y VCDPD LL + IN+LN +++ +N + C P P P IA+ALVN++K + + +L+ AS+FTY L+++W + S C SDK+IIFYSK DG +YT+AG L+ KLPS++IID AV S+ FS G A GL ++++RY LGN Sbjct: 28 YPHEFTAQVQSYPNIMRESAQCLPLKGLYDHDQTFVCDPDRLLTVDQINHLNSMLKEFRNLRSRDESMCSPHSPHPVIALALVNKLKVGNENPDRLLSYASIFTYYLFNEWNLPSTCHTGSDKVIIFYSKDDGVIYTFAGNLLERKLPSQRIIDFAVESRIHFSNGIAAGLGFLIQRYRMLCRSFCASALGN 219
BLAST of SMED30014371 vs. TrEMBL
Match: A0A419QCC8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_23s PE=4 SV=1) HSP 1 Score: 172.555 bits (436), Expect = 2.479e-47 Identity = 86/196 (43.88%), Postives = 123/196 (62.76%), Query Frame = 3 Query: 108 LFGLFLVYSSIPSAFSSTYSPEFTKQVQEYPNVVTDLKKCVP-QGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFC--TSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERY 686 LFGL L + Y EFT QVQ YPN++ + +C+P +G Y VCDPD LL + IN+LN +++ +N + C P P P IA+ALVN++K + + +L+ AS+FTY L+++W + S C SDK+IIFYSK DG +YT+AG L+ KLPS++IID AV S+ FS G A GL ++++RY Sbjct: 20 LFGLTL----------AEYPHEFTAQVQSYPNIMRESAQCLPLKGLYDHDQTFVCDPDRLLTVDQINHLNSMLKEFRNLRSRDESMCSPHSPHPVIALALVNKLKVGNENPDRLLSYASIFTYYLFNEWNLPSTCHTGSDKVIIFYSKDDGVIYTFAGNLLERKLPSQRIIDFAVESRIHFSNGIAAGLGFLIQRY 205
BLAST of SMED30014371 vs. TrEMBL
Match: A0A183AD73 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS4908 PE=4 SV=1) HSP 1 Score: 158.303 bits (399), Expect = 4.286e-44 Identity = 85/206 (41.26%), Postives = 124/206 (60.19%), Query Frame = 3 Query: 78 FMMIMHWNSVLFGLFLVYSSIPSAFSSTYSPEFTKQVQEYPNVVTDLKKCVP-QGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFC--TSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERY 686 F++++ W GLF + Y PEFT QVQ YPNV+ +C+P +GNY VCDP LL + IN LNK +QD +Y ++ S +C P P I +AL N +K ++ + +L+ AS+F Y L+ +W + C SDK+IIFYSK DG LYT+AG L+ KL S+++IDIAV S+ +FS G G+ Y++++Y Sbjct: 8 FLLLITW----IGLF------GHVIQAEYPPEFTHQVQNYPNVIDSKDQCLPLKGNYDHPTTFVCDPFRLLTVDQINRLNKLLQD--DYSRDGS-QCSVTNPNPVIGVALTNRLKVGNESPDRLLDYASIFAYYLFREWNLPFKCHTGSDKIIIFYSKDDGVLYTFAGDLIERKLSSQQLIDIAVQSRVYFSNGIYEGIAYLIQQY 200
BLAST of SMED30014371 vs. Ensembl Nematostella
Match: EDO30764 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZU4]) HSP 1 Score: 63.1586 bits (152), Expect = 2.486e-12 Identity = 45/158 (28.48%), Postives = 74/158 (46.84%), Query Frame = 3 Query: 171 EFTKQVQEYPNVVTDLKKCVPQGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPD-KPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFCTSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFF 641 E++K+ +PN + D + C +G + +CDPD +L D N L + + +N +++ + C+ KP IA+AL+ +IK + S + LA F L+D W + D + S D +Y G + E L S +I I KPF Sbjct: 4 EWSKE--NFPNPIKDTEMCGRRG----VSSWICDPDEVLSYADANTLEEML---RNIEQKTTSGCEHSRKPGYQIAVALMKKIKHHYGISGE--ELARDFAVHLHDSWGVGHRGCGDGAVFLLSVTDRTMYISTGRVAKEVLTSDQIGIIFDEMKPFL 150
BLAST of SMED30014371 vs. Planmine SMEST
Match: SMESG000077296.1 (SMESG000077296.1) HSP 1 Score: 432.18 bits (1110), Expect = 2.050e-155 Identity = 211/213 (99.06%), Postives = 211/213 (99.06%), Query Frame = 3 Query: 90 MHWNSVLFGLFLVYSSIPSAFSSTYSPEFTKQVQEYPNVVTDLKKCVPQGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSFCTSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKAFLGRDKGVLGN 728 MHWNSVLFGLFLVYSSIPS FSSTYSPEFTKQVQEYPNVVTDLKKCVPQGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISS CTSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKAFLGRDKGVLGN Sbjct: 1 MHWNSVLFGLFLVYSSIPSTFSSTYSPEFTKQVQEYPNVVTDLKKCVPQGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNEIKSNKKTSEDILNLASVFTYSLYDDWKISSVCTSDKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKAFLGRDKGVLGN 213
BLAST of SMED30014371 vs. Planmine SMEST
Match: SMESG000021837.1 (SMESG000021837.1) HSP 1 Score: 133.265 bits (334), Expect = 5.478e-38 Identity = 76/207 (36.71%), Postives = 118/207 (57.00%), Query Frame = 3 Query: 105 VLFGLF-LVYSSIPSAFSSTYSPEFTKQVQEYPNVVTDLKKCVP-QGNYHSQVNNVCDPDNLLLIGDINYLNKKIQDIKNYDKENSRECDPDKPRPSIAIALVNE--IKSNKKTSEDILNLASVFTYSLYDDWKISSFCTS--DKLIIFYSKKDGALYTYAGTLMNEKLPSKKIIDIAVASKPFFSLGAARGLKYVLERYSKAFLGR 707 ++FGLF L+ + P + +Q+Q YP+++T KC P +GNY S + VCDPD LL + IN LN I+ ++ +C + P+P+IAIALV + I N K E++++ S+F+YSL+ +W + C S DK+IIFYSK D LY + G + +L + K+++I SKP F+ G +GL V+ R + R Sbjct: 7 LIFGLFSLIVAESPI--------DLDEQIQGYPHIITQTSKCRPIEGNYKSLITYVCDPDKLLDVNQINSLNDMIESVRKDINVGEPDCSAENPKPTIAIALVKKMIIPENSKV-EELMSFGSLFSYSLFTNWNLQVNCKSDPDKIIIFYSKDDSVLYIFVGKKLKARLDTNKLMEIINDSKPAFTNGLYQGLTAVIARLKDTMIRR 204 The following BLAST results are available for this feature:
BLAST of SMED30014371 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30014371 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30014371 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30014371 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30014371 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30014371 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30014371 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014371 ID=SMED30014371|Name=SMED30014371|organism=Schmidtea mediterranea sexual|type=transcript|length=853bpback to top protein sequence of SMED30014371-orf-1 >SMED30014371-orf-1 ID=SMED30014371-orf-1|Name=SMED30014371-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=217bp MMIMHWNSVLFGLFLVYSSIPSAFSSTYSPEFTKQVQEYPNVVTDLKKCVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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