SMED30014358

Overview
NameSMED30014358
Smed IDSMED30014358
Length (bp)1205
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014358 (SMED30014358) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014358 (SMED30014358) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014358

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30014358h1SMcG0000564 Contig1864uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014358h1SMcG0000564 Contig1864newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014358h1SMcG0016265 Contig1864uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014358h1SMcG0016265 Contig1864newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30014358h1SMcG0000564 Contig1864uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014358h1SMcG0000564 Contig1864newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014358h1SMcG0016265 Contig1864uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014358h1SMcG0016265 Contig1864newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30014358h1SMcG0016265 dd_Smed_v4_11780_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014358 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002024:33141..53567 -5821
Homology
[Close]
BLAST of SMED30014358 vs. TrEMBL
Match: R7U9B9 (Uncharacterized protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_51336 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 5.676e-8
Identity = 46/155 (29.68%), Postives = 76/155 (49.03%), Query Frame = 3 
Query:  456 TNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRL-SREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKI-----NGEKHKVIKQFHVTTTVANNEPVYK-SFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISID 899
            TN  +YI S  P K+++ +F RMIW+ KV+ +  LT L        + +WP   N    + E+ +  L  KE+V   +  +R +++     NG +H  + Q+H TT   +  P +  +  RLF +L  V     PI+ H S     +   LI++D
Sbjct:   27 TNGRKYIASQGPKKKTLTDFWRMIWEQKVERIAMLTNLVENGAIKCVRYWPKEVNGDPLKSERFTVKLL-KEDVW-PDFTRRQMEVSKKGSNGARH--VTQYHYTTWPDHGVPSHATALWRLFRKLTHVANNTEPIIVHCS-AGVGRTGTLIAMD 176
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BLAST of SMED30014358 vs. TrEMBL
Match: R7VKM1 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_220101 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.697e-6
Identity = 44/145 (30.34%), Postives = 71/145 (48.97%), Query Frame = 3 
Query:  456 TNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHG-IHFWPALNN----TIQFQFE-----KDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKS-FKRLFIQLNEVKETDYPILFHTS 857
            TN  +YI S  P  +S+ +F RMIW+ KV+ +  LT L  +  +  + +WP   N     I  QFE     +D +  FT+ +++       +IK N  + + + Q+H TT   +  P + +   RLF +L    +   PIL H S
Sbjct:  862 TNGRKYIASQGPKPKSLTDFWRMIWEQKVERIAMLTNLVEKGNNKCMQYWPEQVNGDPLKIHSQFEINLLKEDVWPDFTRRQME-------VIKKNSNRARHVTQYHYTTWPDHGVPSHATALWRLFRKLTNDADNTQPILIHCS 999
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BLAST of SMED30014358 vs. Planmine SMEST
Match: SMESG000068926.1 (SMESG000068926.1)

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 336/338 (99.41%), Postives = 336/338 (99.41%), Query Frame = 3 
Query:   54 MFXXXXXXXXXXXXRFLSMKTETTEIVLETENLYQYLEEIKNYSIPRNEFHDIYESLLKDRYIPLNKEFKNLVEISMQINQEQXXXXXXXXXXXXXFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKXXXXXXXXXXXXXXXXTVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV 1067
            MFPKSLSPKSPLLKRFLSMKTE TEIVLETENLYQYLEEIKNYSIPRNEFHDIYE LLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYETVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV
Sbjct:    1 MFPKSLSPKSPLLKRFLSMKTEITEIVLETENLYQYLEEIKNYSIPRNEFHDIYEGLLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYETVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV 338
[Close]
BLAST of SMED30014358 vs. Planmine SMEST
Match: SMESG000068926.1 (SMESG000068926.1)

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 336/340 (98.82%), Postives = 336/340 (98.82%), Query Frame = 3 
Query:   54 MFXXXXXXXXXXXXRFLSMKTETTEIVLETENLYQYLEEIKNYSIPRNEFHDIYESLLKDRYIPLNKEFKNLVEISMQINQEQXXXXXXXXXXXXXFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKI--NGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKXXXXXXXXXXXXXXXXTVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV 1067
            MFPKSLSPKSPLLKRFLSMKTE TEIVLETENLYQYLEEIKNYSIPRNEFHDIYE LLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKI  NGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYETVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV
Sbjct:    1 MFPKSLSPKSPLLKRFLSMKTEITEIVLETENLYQYLEEIKNYSIPRNEFHDIYEGLLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKIVKNGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYETVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV 340
[Close]
BLAST of SMED30014358 vs. Planmine SMEST
Match: SMESG000068926.1 (SMESG000068926.1)

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 318/320 (99.38%), Postives = 318/320 (99.38%), Query Frame = 3 
Query:  108 MKTETTEIVLETENLYQYLEEIKNYSIPRNEFHDIYESLLKDRYIPLNKEFKNLVEISMQINQEQXXXXXXXXXXXXXFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKXXXXXXXXXXXXXXXXTVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV 1067
            MKTE TEIVLETENLYQYLEEIKNYSIPRNEFHDIYE LLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYETVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV
Sbjct:    1 MKTEITEIVLETENLYQYLEEIKNYSIPRNEFHDIYEGLLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYETVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV 320
[Close]
BLAST of SMED30014358 vs. Planmine SMEST
Match: SMESG000068931.1 (SMESG000068931.1)

HSP 1 Score: 165.236 bits (417), Expect = 3.791e-47
Identity = 108/324 (33.33%), Postives = 194/324 (59.88%), Query Frame = 3 
Query:   96 RFLSMKTETTEIVLETENLYQYLEEIKNYSIPRNEFHDIYESLLKDRYIPLNKEFKNLVEISMQINQEQXXXXXXXXXXXXXFDHQKQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKR--IIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEVKETD--YPILFHTSNLNREQCSMLISIDKXXXXXXXXXXXXXXXXTVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSK 1055
            R +S K  +++   E     Q   ++ N +I  +EF + Y+ L  + ++ L KE+K+L+  SM + + + ++ + + ++S++  H +   +Q  +L+V +S S +S++ LD  +I +  +   IQYI+S DPS++SI +F +MIW+  + Y++TLT++S+ K     +W + +    +  +K S+ +   +E    NL +R  I++ N  + + I Q H+ T+   N+ V   +++LFI+L+E+K     YP+LFH+SN N +QC+MLI IDKII +L    + +NIY  + ++NQY+N ML +E  Y  IYEYI  +L+K  K
Sbjct:   15 RMMSQKKRSSKHKTEEPQPVQEEAKVLNRAIRISEFKNEYKKLCCNDFVILKKEYKDLINESMTMKESEYNIWTSIESMSNINLHDRIHIVQVDNLRVTMSSSLFSNAKLDAVFIPDQVKHQVIQYIVSQDPSEDSIFDFFQMIWELNISYLITLTKISKRKGFIYEYWDSKSENKTYSKDKVSYKIKKTKETNSGNLTERLFILENNNLECREITQLHLMTSTCQNDSVLNYYEKLFIKLHEIKSLGLCYPLLFHSSNSNLDQCAMLICIDKIISTLAFGSDMVNIYRMMLELNQYRNLMLQQECHYLYIYEYIDWFLRKSFK 338
[Close]
BLAST of SMED30014358 vs. Planmine SMEST
Match: SMESG000068923.1 (SMESG000068923.1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.307e-20
Identity = 67/234 (28.63%), Postives = 120/234 (51.28%), Query Frame = 3 
Query:  366 LQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKESIVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFSLFTKEEVQRKNLVKRIIKING---EKHKVIKQFHVTTTVANNEPVYKSFKRLFIQLNEV---KETDYPILFHTSNLNREQCSMLISIDKXXXXXXXXXXXXXXXXTVFQMNQYKNSMLVEERQYFDIYEYISCYLKKQ 1049
            LQ  D+ V   +  +S+  L   YI+     N ++YI S DP  ES+ +FL+MIW+ K+  +V +  L   +     +WP    +  +  +   + + T+ E    N++ R + I     E+ ++I Q  ++     NE    +F  L +++ EV   K+  + ILFH    N   C++LI +D+II S+E      N+YETV ++ Q ++ ++  E QY +IY+ +  +LK +
Sbjct:   62 LQVKDINVISKKLPFSNQNLKANYIYQTINENLLEYIASNDPDYESVSDFLQMIWECKITLIVRIKELYETQNDTCEYWPPNLESNNYCNQSSCYKIKTQNESISNNIIIRKLLIQNVKYEETRIIVQIEISIDTKLNENTASTFDSLLLEIQEVKSIKDRSHRILFHARENNLHHCAVLICLDQIITSVEAGISEFNVYETVLRICQSRSMLIKNENQYINIYQSVYRFLKSK 295
The following BLAST results are available for this feature:
BLAST of SMED30014358 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 2
Match NameE-valueIdentityDescription
R7U9B95.676e-829.68Uncharacterized protein (Fragment) OS=Capitella te... [more]
R7VKM12.697e-630.34Uncharacterized protein OS=Capitella teleta OX=283... [more]
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BLAST of SMED30014358 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014358 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000068926.10.000e+099.41SMESG000068926.1[more]
SMESG000068926.10.000e+098.82SMESG000068926.1[more]
SMESG000068926.10.000e+099.38SMESG000068926.1[more]
SMESG000068931.13.791e-4733.33SMESG000068931.1[more]
SMESG000068923.11.307e-2028.63SMESG000068923.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014358 ID=SMED30014358|Name=SMED30014358|organism=Schmidtea mediterranea sexual|type=transcript|length=1205bp
GACAAATTTCATTAGTATGATTCGATTTCATTAATAAAATAGATATTATT
GCGATGTTCCCAAAATCCTTATCACCAAAATCGCCATTACTAAAACGATT
TCTTTCCATGAAAACGGAAACAACTGAAATTGTTTTGGAAACAGAGAACC
TCTATCAGTACCTGGAAGAAATAAAAAATTATTCAATTCCTAGGAATGAG
TTTCACGATATTTACGAAAGTCTTCTCAAAGACAGATACATTCCTTTGAA
TAAAGAATTTAAAAACTTGGTTGAAATCTCTATGCAAATTAATCAAGAAC
AATCTAGCCTACTCAGTATACTGTCCAACATAAGTTCCTTGTTTGACCAT
CAAAAGCAGATCACTCTGCAAGCTTTGGATTTGAAAGTATACATATCCCA
ATCGTTTTATTCCAGCAGCGGACTAGATTGTACATATATACACAATGATA
GACAAACGAATTGCATTCAGTACATCATATCAACCGATCCATCCAAAGAG
AGTATTGTAGAATTTCTTCGAATGATCTGGAAATCAAAAGTAAAATATGT
GGTAACCCTCACCAGGCTCAGTAGGGAGAAGTTTCACGGAATTCATTTTT
GGCCAGCATTAAACAATACAATACAATTTCAATTTGAAAAAGATTCATTC
AGCCTCTTTACAAAAGAAGAAGTTCAAAGAAAAAACTTAGTGAAAAGAAT
CATAAAAATTAATGGAGAAAAACATAAAGTCATCAAGCAGTTCCACGTTA
CAACGACGGTAGCTAATAACGAACCCGTGTATAAATCGTTTAAAAGGCTT
TTTATCCAACTAAACGAGGTCAAGGAAACAGATTACCCAATTTTATTTCA
TACCAGTAACCTAAACCGAGAACAGTGCTCGATGCTCATTTCCATTGACA
AGATTATTAAAAGTTTGGAAAACAACGAAGAAAATATAAATATCTACGAA
ACCGTTTTTCAAATGAATCAATATAAGAACAGTATGCTAGTTGAAGAGCG
CCAGTATTTCGACATCTATGAATACATCAGCTGCTATTTGAAGAAACAAT
CTAAATATTATGACGTTTAGATGATTGAATAAGAATCTTAAACCCAGAAA
TCTACTGATAAACTATAATTATTTTTATTTTAAATTATCAGATTTACTTT
TTGAATTTCTTTAATGAAATTTGTATTTTCTTTTTGGACAGAATTTCATG
AAAGC
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protein sequence of SMED30014358-orf-1

>SMED30014358-orf-1 ID=SMED30014358-orf-1|Name=SMED30014358-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=339bp
MFPKSLSPKSPLLKRFLSMKTETTEIVLETENLYQYLEEIKNYSIPRNEF
HDIYESLLKDRYIPLNKEFKNLVEISMQINQEQSSLLSILSNISSLFDHQ
KQITLQALDLKVYISQSFYSSSGLDCTYIHNDRQTNCIQYIISTDPSKES
IVEFLRMIWKSKVKYVVTLTRLSREKFHGIHFWPALNNTIQFQFEKDSFS
LFTKEEVQRKNLVKRIIKINGEKHKVIKQFHVTTTVANNEPVYKSFKRLF
IQLNEVKETDYPILFHTSNLNREQCSMLISIDKIIKSLENNEENINIYET
VFQMNQYKNSMLVEERQYFDIYEYISCYLKKQSKYYDV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR029021Prot-tyrosine_phosphatase-like
IPR003595Tyr_Pase_cat
IPR000242PTPase_domain
Vocabulary: molecular function
TermDefinition
GO:0004725protein tyrosine phosphatase activity
Vocabulary: biological process
TermDefinition
GO:0006470protein dephosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000242PTP type protein phosphataseSMARTSM00194PTPc_3coord: 32..330
e-value: 1.5E-4
score: -37.0
IPR000242PTP type protein phosphatasePFAMPF00102Y_phosphatasecoord: 128..325
e-value: 9.1E-17
score: 61.4
IPR000242PTP type protein phosphatasePROSITEPS50055TYR_PHOSPHATASE_PTPcoord: 80..328
score: 16.08
IPR003595Protein-tyrosine phosphatase, catalyticSMARTSM00404ptp_7coord: 226..327
e-value: 0.0052
score: 15.4
IPR029021Protein-tyrosine phosphatase-likeGENE3DG3DSA:3.90.190.10coord: 26..337
e-value: 1.1E-22
score: 82.7
IPR029021Protein-tyrosine phosphatase-likeSUPERFAMILYSSF52799(Phosphotyrosine protein) phosphatases IIcoord: 132..336
NoneNo IPR availablePANTHERPTHR19134PROTEIN-TYROSINE PHOSPHATASEcoord: 113..331
NoneNo IPR availableCDDcd00047PTPccoord: 128..323
e-value: 7.1738E-17
score: 75.7848