Four and A half lim domains
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Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014320 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 356.295 bits (913), Expect = 2.624e-119 Identity = 158/273 (57.88%), Postives = 207/273 (75.82%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YIL+EE+P+C+ C+ET FANTCE+C + IGCD KDLS+KDRHWHE CF C +C SLVD+PFA K++ L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CVPCYE ++A +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++DD YC +C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 8.988e-38 Identity = 83/273 (30.40%), Postives = 126/273 (46.15%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y YC + C C + I + + H H F C C NSL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ + A +C C K G YR + WH+ECF+C C++ + + F RD C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 3.207e-28 Identity = 72/247 (29.15%), Postives = 116/247 (46.96%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C EC IG +LS + H WH ACF+CS+C+ LVD + ++L C Y + +C C + I TR M+ H F C C + +I K+ C+ CYE + A C QCK+ I + ++++++ WH++CF C C L + F +D+ +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 40 CEECGKPIGCDCKDLSYKDRH-------WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 356.295 bits (913), Expect = 2.624e-119 Identity = 158/273 (57.88%), Postives = 207/273 (75.82%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YIL+EE+P+C+ C+ET FANTCE+C + IGCD KDLS+KDRHWHE CF C +C SLVD+PFA K++ L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CVPCYE ++A +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++DD YC +C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 8.988e-38 Identity = 83/273 (30.40%), Postives = 126/273 (46.15%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y YC + C C + I + + H H F C C NSL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ + A +C C K G YR + WH+ECF+C C++ + + F RD C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 3.207e-28 Identity = 72/247 (29.15%), Postives = 116/247 (46.96%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C EC IG +LS + H WH ACF+CS+C+ LVD + ++L C Y + +C C + I TR M+ H F C C + +I K+ C+ CYE + A C QCK+ I + ++++++ WH++CF C C L + F +D+ +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 40 CEECGKPIGCDCKDLSYKDRH-------WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 356.295 bits (913), Expect = 2.624e-119 Identity = 158/273 (57.88%), Postives = 207/273 (75.82%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YIL+EE+P+C+ C+ET FANTCE+C + IGCD KDLS+KDRHWHE CF C +C SLVD+PFA K++ L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CVPCYE ++A +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++DD YC +C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 8.988e-38 Identity = 83/273 (30.40%), Postives = 126/273 (46.15%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y YC + C C + I + + H H F C C NSL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ + A +C C K G YR + WH+ECF+C C++ + + F RD C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 3.207e-28 Identity = 72/247 (29.15%), Postives = 116/247 (46.96%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C EC IG +LS + H WH ACF+CS+C+ LVD + ++L C Y + +C C + I TR M+ H F C C + +I K+ C+ CYE + A C QCK+ I + ++++++ WH++CF C C L + F +D+ +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 40 CEECGKPIGCDCKDLSYKDRH-------WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 356.295 bits (913), Expect = 2.624e-119 Identity = 158/273 (57.88%), Postives = 207/273 (75.82%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YIL+EE+P+C+ C+ET FANTCE+C + IGCD KDLS+KDRHWHE CF C +C SLVD+PFA K++ L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CVPCYE ++A +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++DD YC +C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 8.988e-38 Identity = 83/273 (30.40%), Postives = 126/273 (46.15%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y YC + C C + I + + H H F C C NSL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ + A +C C K G YR + WH+ECF+C C++ + + F RD C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 3.207e-28 Identity = 72/247 (29.15%), Postives = 116/247 (46.96%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C EC IG +LS + H WH ACF+CS+C+ LVD + ++L C Y + +C C + I TR M+ H F C C + +I K+ C+ CYE + A C QCK+ I + ++++++ WH++CF C C L + F +D+ +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 40 CEECGKPIGCDCKDLSYKDRH-------WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 356.295 bits (913), Expect = 2.624e-119 Identity = 158/273 (57.88%), Postives = 207/273 (75.82%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YIL+EE+P+C+ C+ET FANTCE+C + IGCD KDLS+KDRHWHE CF C +C SLVD+PFA K++ L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CVPCYE ++A +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++DD YC +C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 8.988e-38 Identity = 83/273 (30.40%), Postives = 126/273 (46.15%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y YC + C C + I + + H H F C C NSL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ + A +C C K G YR + WH+ECF+C C++ + + F RD C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 3.207e-28 Identity = 72/247 (29.15%), Postives = 116/247 (46.96%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C EC IG +LS + H WH ACF+CS+C+ LVD + ++L C Y + +C C + I TR M+ H F C C + +I K+ C+ CYE + A C QCK+ I + ++++++ WH++CF C C L + F +D+ +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 40 CEECGKPIGCDCKDLSYKDRH-------WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 535.028 bits (1377), Expect = 0.000e+0 Identity = 252/517 (48.74%), Postives = 345/517 (66.73%), Query Frame = 1
Query: 208 GFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKAS---GLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G++ H WRK C C H++ + V + + + + + A+ + S G W+P GL +VE ++S LP + P + +K R ++L Q+P QD + HL + ER+ E+F+ RN L I +V + + ++C +C + +E++V I + WHPACF C C++LLVD YC N++YC RHY E +PRC+ CDELIF EYT+AM+++ H +HF C CD +LTG RYI+++E P+CIKCYE FAN C++C + IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ +FCS CYD FA RCDGC ++F+AGM+K EY+GKQWH++CF C CK IG KSFIP++ + C PCYE K+A +CSKC +VI GGVTYK PWH+ECF CTNC LAG +FTSKD+KPYCA+CYG+LFAK+C CTKPITG GG KFISFE RHWH++CF C++CTT+LVG+GF+T+ I+CP+C +
Sbjct: 271 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNIN-KAMECHKCSGILETNEMAV-IAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 785
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 535.028 bits (1377), Expect = 0.000e+0 Identity = 252/517 (48.74%), Postives = 345/517 (66.73%), Query Frame = 1
Query: 208 GFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKAS---GLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G++ H WRK C C H++ + V + + + + + A+ + S G W+P GL +VE ++S LP + P + +K R ++L Q+P QD + HL + ER+ E+F+ RN L I +V + + ++C +C + +E++V I + WHPACF C C++LLVD YC N++YC RHY E +PRC+ CDELIF EYT+AM+++ H +HF C CD +LTG RYI+++E P+CIKCYE FAN C++C + IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ +FCS CYD FA RCDGC ++F+AGM+K EY+GKQWH++CF C CK IG KSFIP++ + C PCYE K+A +CSKC +VI GGVTYK PWH+ECF CTNC LAG +FTSKD+KPYCA+CYG+LFAK+C CTKPITG GG KFISFE RHWH++CF C++CTT+LVG+GF+T+ I+CP+C +
Sbjct: 241 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNIN-KAMECHKCSGILETNEMAV-IAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 755
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 533.102 bits (1372), Expect = 0.000e+0 Identity = 252/517 (48.74%), Postives = 345/517 (66.73%), Query Frame = 1
Query: 208 GFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKAS---GLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G++ H WRK C C H++ + V + + + + + A+ + S G W+P GL +VE ++S LP + P + +K R ++L Q+P QD + HL + ER+ E+F+ RN L I +V + + ++C +C + +E++V I + WHPACF C C++LLVD YC N++YC RHY E +PRC+ CDELIF EYT+AM+++ H +HF C CD +LTG RYI+++E P+CIKCYE FAN C++C + IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ +FCS CYD FA RCDGC ++F+AGM+K EY+GKQWH++CF C CK IG KSFIP++ + C PCYE K+A +CSKC +VI GGVTYK PWH+ECF CTNC LAG +FTSKD+KPYCA+CYG+LFAK+C CTKPITG GG KFISFE RHWH++CF C++CTT+LVG+GF+T+ I+CP+C +
Sbjct: 134 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNIN-KAMECHKCSGILETNEMAV-IAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 648
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 531.561 bits (1368), Expect = 0.000e+0 Identity = 252/517 (48.74%), Postives = 345/517 (66.73%), Query Frame = 1
Query: 208 GFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKAS---GLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G++ H WRK C C H++ + V + + + + + A+ + S G W+P GL +VE ++S LP + P + +K R ++L Q+P QD + HL + ER+ E+F+ RN L I +V + + ++C +C + +E++V I + WHPACF C C++LLVD YC N++YC RHY E +PRC+ CDELIF EYT+AM+++ H +HF C CD +LTG RYI+++E P+CIKCYE FAN C++C + IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ +FCS CYD FA RCDGC ++F+AGM+K EY+GKQWH++CF C CK IG KSFIP++ + C PCYE K+A +CSKC +VI GGVTYK PWH+ECF CTNC LAG +FTSKD+KPYCA+CYG+LFAK+C CTKPITG GG KFISFE RHWH++CF C++CTT+LVG+GF+T+ I+CP+C +
Sbjct: 42 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNIN-KAMECHKCSGILETNEMAV-IAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 556
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 531.561 bits (1368), Expect = 0.000e+0 Identity = 252/517 (48.74%), Postives = 345/517 (66.73%), Query Frame = 1
Query: 208 GFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKAS---GLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G++ H WRK C C H++ + V + + + + + A+ + S G W+P GL +VE ++S LP + P + +K R ++L Q+P QD + HL + ER+ E+F+ RN L I +V + + ++C +C + +E++V I + WHPACF C C++LLVD YC N++YC RHY E +PRC+ CDELIF EYT+AM+++ H +HF C CD +LTG RYI+++E P+CIKCYE FAN C++C + IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ +FCS CYD FA RCDGC ++F+AGM+K EY+GKQWH++CF C CK IG KSFIP++ + C PCYE K+A +CSKC +VI GGVTYK PWH+ECF CTNC LAG +FTSKD+KPYCA+CYG+LFAK+C CTKPITG GG KFISFE RHWH++CF C++CTT+LVG+GF+T+ I+CP+C +
Sbjct: 42 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNIN-KAMECHKCSGILETNEMAV-IAPKLGDSTGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 556
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302779) HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0 Identity = 258/527 (48.96%), Postives = 337/527 (63.95%), Query Frame = 1
Query: 172 GSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPI-EVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G C +C C GF H WRKTC +C C + H ++ + V + + S +EA+ G W+P G++ + + LP P + SR R++ Q+P QD L+ HL+ E E F+ RN L IA+++ + C C I EL V WHP CF CS C LLVD YC H +K+YC RHY E ++PRCA CDELIF+ EYT+AMD++ H HF C CD SLTG RY++++++P+CIKCYE FANTCE+C + IG DSKDLS+KD+HWHE CF CF+CH SLVD+ F K + ++C CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C CK IG KSFIPR+Q+I C CYE K+A +C KC +VI GGVTYK PWH+ECFTCT+C LAG +FTS+D+KPYCA+C+GELFAK+CT C KPITG GG +FISFE RHWH +CF C+ C +LVGRGF+T+ +I+CPDC +
Sbjct: 37 GVRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVEARDLG----FTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE--HCAHCDNEIAAGELVVAAPKFVESV-MWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 555 HSP 2 Score: 133.265 bits (334), Expect = 1.016e-32 Identity = 81/251 (32.27%), Postives = 122/251 (48.61%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENK 1389
C EC IG +LS + H WH ACF C +C LVD + +K+YC Y + RC C E+ T+ M+ ++ H N F C C ++ +I +E+ +C CYE KFA C +C + I S +++K+ WH +CF C C+ +L + F ++DE +C+EC+ FA RC C K G R + + WH +CF+C CK + + FI I+C C + K
Sbjct: 318 CEECNKIIGIDSKDLSYKDKH-------WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302781) HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0 Identity = 258/527 (48.96%), Postives = 337/527 (63.95%), Query Frame = 1
Query: 172 GSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPI-EVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G C +C C GF H WRKTC +C C + H ++ + V + + S +EA+ G W+P G++ + + LP P + SR R++ Q+P QD L+ HL+ E E F+ RN L IA+++ + C C I EL V WHP CF CS C LLVD YC H +K+YC RHY E ++PRCA CDELIF+ EYT+AMD++ H HF C CD SLTG RY++++++P+CIKCYE FANTCE+C + IG DSKDLS+KD+HWHE CF CF+CH SLVD+ F K + ++C CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C CK IG KSFIPR+Q+I C CYE K+A +C KC +VI GGVTYK PWH+ECFTCT+C LAG +FTS+D+KPYCA+C+GELFAK+CT C KPITG GG +FISFE RHWH +CF C+ C +LVGRGF+T+ +I+CPDC +
Sbjct: 37 GVRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVEARDLG----FTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE--HCAHCDNEIAAGELVVAAPKFVESV-MWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 555 HSP 2 Score: 133.265 bits (334), Expect = 1.016e-32 Identity = 81/251 (32.27%), Postives = 122/251 (48.61%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENK 1389
C EC IG +LS + H WH ACF C +C LVD + +K+YC Y + RC C E+ T+ M+ ++ H N F C C ++ +I +E+ +C CYE KFA C +C + I S +++K+ WH +CF C C+ +L + F ++DE +C+EC+ FA RC C K G R + + WH +CF+C CK + + FI I+C C + K
Sbjct: 318 CEECNKIIGIDSKDLSYKDKH-------WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0306704) HSP 1 Score: 527.709 bits (1358), Expect = 0.000e+0 Identity = 258/527 (48.96%), Postives = 337/527 (63.95%), Query Frame = 1
Query: 172 GSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPI-EVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
G C +C C GF H WRKTC +C C + H ++ + V + + S +EA+ G W+P G++ + + LP P + SR R++ Q+P QD L+ HL+ E E F+ RN L IA+++ + C C I EL V WHP CF CS C LLVD YC H +K+YC RHY E ++PRCA CDELIF+ EYT+AMD++ H HF C CD SLTG RY++++++P+CIKCYE FANTCE+C + IG DSKDLS+KD+HWHE CF CF+CH SLVD+ F K + ++C CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C CK IG KSFIPR+Q+I C CYE K+A +C KC +VI GGVTYK PWH+ECFTCT+C LAG +FTS+D+KPYCA+C+GELFAK+CT C KPITG GG +FISFE RHWH +CF C+ C +LVGRGF+T+ +I+CPDC +
Sbjct: 37 GVRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVEARDLG----FTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE--HCAHCDNEIAAGELVVAAPKFVESV-MWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 555 HSP 2 Score: 133.265 bits (334), Expect = 1.016e-32 Identity = 81/251 (32.27%), Postives = 122/251 (48.61%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENK 1389
C EC IG +LS + H WH ACF C +C LVD + +K+YC Y + RC C E+ T+ M+ ++ H N F C C ++ +I +E+ +C CYE KFA C +C + I S +++K+ WH +CF C C+ +L + F ++DE +C+EC+ FA RC C K G R + + WH +CF+C CK + + FI I+C C + K
Sbjct: 318 CEECNKIIGIDSKDLSYKDKH-------WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0332703) HSP 1 Score: 510.76 bits (1314), Expect = 2.253e-176 Identity = 250/508 (49.21%), Postives = 328/508 (64.57%), Query Frame = 1
Query: 229 RKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPI-EVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
RKTC +C C + H ++ + V + + S +EA+ G W+P G++ + + LP P + SR R++ Q+P QD L+ HL+ E E F+ RN L IA+++ + C C I EL V WHP CF CS C LLVD YC H +K+YC RHY E ++PRCA CDELIF+ EYT+AMD++ H HF C CD SLTG RY++++++P+CIKCYE FANTCE+C + IG DSKDLS+KD+HWHE CF CF+CH SLVD+ F K + ++C CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C CK IG KSFIPR+Q+I C CYE K+A +C KC +VI GGVTYK PWH+ECFTCT+C LAG +FTS+D+KPYCA+C+GELFAK+CT C KPITG GG +FISFE RHWH +CF C+ C +LVGRGF+T+ +I+CPDC +
Sbjct: 26 RKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVEARDLG----FTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE--HCAHCDNEIAAGELVVAAPKFVESV-MWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 525 HSP 2 Score: 133.265 bits (334), Expect = 7.213e-33 Identity = 81/251 (32.27%), Postives = 122/251 (48.61%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENK 1389
C EC IG +LS + H WH ACF C +C LVD + +K+YC Y + RC C E+ T+ M+ ++ H N F C C ++ +I +E+ +C CYE KFA C +C + I S +++K+ WH +CF C C+ +L + F ++DE +C+EC+ FA RC C K G R + + WH +CF+C CK + + FI I+C C + K
Sbjct: 288 CEECNKIIGIDSKDLSYKDKH-------WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 527
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302780) HSP 1 Score: 511.146 bits (1315), Expect = 3.871e-176 Identity = 250/508 (49.21%), Postives = 328/508 (64.57%), Query Frame = 1
Query: 229 RKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPI-EVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
RKTC +C C + H ++ + V + + S +EA+ G W+P G++ + + LP P + SR R++ Q+P QD L+ HL+ E E F+ RN L IA+++ + C C I EL V WHP CF CS C LLVD YC H +K+YC RHY E ++PRCA CDELIF+ EYT+AMD++ H HF C CD SLTG RY++++++P+CIKCYE FANTCE+C + IG DSKDLS+KD+HWHE CF CF+CH SLVD+ F K + ++C CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C CK IG KSFIPR+Q+I C CYE K+A +C KC +VI GGVTYK PWH+ECFTCT+C LAG +FTS+D+KPYCA+C+GELFAK+CT C KPITG GG +FISFE RHWH +CF C+ C +LVGRGF+T+ +I+CPDC +
Sbjct: 55 RKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVEARDLG----FTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE--HCAHCDNEIAAGELVVAAPKFVESV-MWHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 554 HSP 2 Score: 133.265 bits (334), Expect = 8.833e-33 Identity = 81/251 (32.27%), Postives = 122/251 (48.61%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENK 1389
C EC IG +LS + H WH ACF C +C LVD + +K+YC Y + RC C E+ T+ M+ ++ H N F C C ++ +I +E+ +C CYE KFA C +C + I S +++K+ WH +CF C C+ +L + F ++DE +C+EC+ FA RC C K G R + + WH +CF+C CK + + FI I+C C + K
Sbjct: 317 CEECNKIIGIDSKDLSYKDKH-------WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 556
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 355.91 bits (912), Expect = 2.465e-119 Identity = 156/273 (57.14%), Postives = 208/273 (76.19%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L++ENP+C+KCYE+ ++NTCE+CK+ IGC+S+DLS+KDRHWH+ CF CF+CH SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C +C + I GGVTY PWHK+CF CT C +QL+G +FTS+DD PYC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 144.821 bits (364), Expect = 1.050e-38 Identity = 87/273 (31.87%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y YC + Y C C + I N D+ H + F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD S +C CY+ +FA +C C K G Y + WH++CFLC CKQ + + F RD C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 114.39 bits (285), Expect = 5.751e-28 Identity = 73/258 (28.29%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF+C +C LVD + +L C Y + +C C + I +R M+ + + H F C+ C + +I K+ + +C+ CYE +FA C QCK+ I + +++ D+ WH+ CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNSRDLSYKDRH-------WHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 4 Score: 99.7525 bits (247), Expect = 8.583e-23 Identity = 63/204 (30.88%), Postives = 97/204 (47.55%), Query Frame = 1
Query: 589 KNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIG--CDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSEC 1194
K ++ D + E + +CFECK TI S +++H +SWH CF C C++ + + N YC Y ++ +C C + I T DQ H + F C C L+G R+ +++ P+C+ C+ +A C C I SK +SF++R WH CF C +C SLV R F T+ + + C EC
Sbjct: 78 KPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHK---GNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 355.91 bits (912), Expect = 2.465e-119 Identity = 156/273 (57.14%), Postives = 208/273 (76.19%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L++ENP+C+KCYE+ ++NTCE+CK+ IGC+S+DLS+KDRHWH+ CF CF+CH SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C +C + I GGVTY PWHK+CF CT C +QL+G +FTS+DD PYC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 144.821 bits (364), Expect = 1.050e-38 Identity = 87/273 (31.87%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y YC + Y C C + I N D+ H + F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD S +C CY+ +FA +C C K G Y + WH++CFLC CKQ + + F RD C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 114.39 bits (285), Expect = 5.751e-28 Identity = 73/258 (28.29%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF+C +C LVD + +L C Y + +C C + I +R M+ + + H F C+ C + +I K+ + +C+ CYE +FA C QCK+ I + +++ D+ WH+ CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNSRDLSYKDRH-------WHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 4 Score: 99.7525 bits (247), Expect = 8.583e-23 Identity = 63/204 (30.88%), Postives = 97/204 (47.55%), Query Frame = 1
Query: 589 KNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIG--CDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSEC 1194
K ++ D + E + +CFECK TI S +++H +SWH CF C C++ + + N YC Y ++ +C C + I T DQ H + F C C L+G R+ +++ P+C+ C+ +A C C I SK +SF++R WH CF C +C SLV R F T+ + + C EC
Sbjct: 78 KPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHK---GNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 355.91 bits (912), Expect = 2.465e-119 Identity = 156/273 (57.14%), Postives = 208/273 (76.19%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L++ENP+C+KCYE+ ++NTCE+CK+ IGC+S+DLS+KDRHWH+ CF CF+CH SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C +C + I GGVTY PWHK+CF CT C +QL+G +FTS+DD PYC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 144.821 bits (364), Expect = 1.050e-38 Identity = 87/273 (31.87%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y YC + Y C C + I N D+ H + F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD S +C CY+ +FA +C C K G Y + WH++CFLC CKQ + + F RD C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 114.39 bits (285), Expect = 5.751e-28 Identity = 73/258 (28.29%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF+C +C LVD + +L C Y + +C C + I +R M+ + + H F C+ C + +I K+ + +C+ CYE +FA C QCK+ I + +++ D+ WH+ CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNSRDLSYKDRH-------WHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 4 Score: 99.7525 bits (247), Expect = 8.583e-23 Identity = 63/204 (30.88%), Postives = 97/204 (47.55%), Query Frame = 1
Query: 589 KNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIG--CDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSEC 1194
K ++ D + E + +CFECK TI S +++H +SWH CF C C++ + + N YC Y ++ +C C + I T DQ H + F C C L+G R+ +++ P+C+ C+ +A C C I SK +SF++R WH CF C +C SLV R F T+ + + C EC
Sbjct: 78 KPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHK---GNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 353.214 bits (905), Expect = 2.940e-118 Identity = 155/273 (56.78%), Postives = 206/273 (75.46%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L+E+NP+C+KCYE+ ++NTCE+CK+ IGC+S+DLS+KDRHWHE CF CF+C SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C C + I GGVTY PWHK+CF CT C +QL+G +FTS+DD YC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT+ D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECGK 277 HSP 2 Score: 145.206 bits (365), Expect = 6.353e-39 Identity = 86/273 (31.50%), Postives = 127/273 (46.52%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y + YC + Y C C + I N D+ H + F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD +C CY+ +FA +C C K G Y + WH++CFLC CKQ + + F RD C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F + D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 115.161 bits (287), Expect = 3.095e-28 Identity = 73/258 (28.29%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF+C +CK LVD + +L C Y + +C C + I +R M+ + + H F C+ C + +I K+ + +C+ CYE +FA C CK+ I + +++ D+ WH+ CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNSRDLSYKDRH-------WHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 353.214 bits (905), Expect = 2.940e-118 Identity = 155/273 (56.78%), Postives = 206/273 (75.46%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L+E+NP+C+KCYE+ ++NTCE+CK+ IGC+S+DLS+KDRHWHE CF CF+C SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C C + I GGVTY PWHK+CF CT C +QL+G +FTS+DD YC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT+ D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECGK 277 HSP 2 Score: 145.206 bits (365), Expect = 6.353e-39 Identity = 86/273 (31.50%), Postives = 127/273 (46.52%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y + YC + Y C C + I N D+ H + F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD +C CY+ +FA +C C K G Y + WH++CFLC CKQ + + F RD C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F + D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 115.161 bits (287), Expect = 3.095e-28 Identity = 73/258 (28.29%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF+C +CK LVD + +L C Y + +C C + I +R M+ + + H F C+ C + +I K+ + +C+ CYE +FA C CK+ I + +++ D+ WH+ CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNSRDLSYKDRH-------WHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl2 (four and a half LIM domains 2 [Source:NCBI gene;Acc:100145283]) HSP 1 Score: 357.066 bits (915), Expect = 1.139e-119 Identity = 159/273 (58.24%), Postives = 203/273 (74.36%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C C SL G +Y+L+EENP+C+KCYE+ ++N+CE+CK+ IGCD KDLS+KDRHWHE CF CF+C SLVD+PFA KDE L C+ECY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CV CYE ++A C +C + I GGVTY+ PWHKECF CT C K L+G +FTS+D+ YC +C+ L+AKKC CT PI+G GG K+ISFE R WHS+CF C KC +LVGRGFLT D+I+CP+CG+
Sbjct: 5 FDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 151.754 bits (382), Expect = 4.374e-41 Identity = 87/273 (31.87%), Postives = 130/273 (47.62%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C CKE L Y YC + Y C C + I + + H H + F C C SL + K+E+ C +CY ++++ C +CK+ I ++ + +K WHE CF C C + + F KD FC CY+ +FA C C K G YR + WH+ECF+C CK+P+ + F RD+ C+ C+ N YA+KC+ CT I G ++++ WH +CF C C L G F ++ D C +C
Sbjct: 5 FDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTG--GVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275 HSP 3 Score: 122.865 bits (307), Expect = 1.066e-30 Identity = 77/258 (29.84%), Postives = 122/258 (47.29%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH +CF+C +CK+ LVD + L C Y + +C+ C + I TR M+ + + H F C C + +I K+ C+ CYE +FA C QCK+ I + ++++D+ WH++CF C C L + F ++DE +C C+ N +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNSCEECKKPIGCDGKDLSYKDRH-------WHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPG--TRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAI--TTGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: shroom3 (shroom family member 3 [Source:Xenbase;Acc:XB-GENE-490224]) HSP 1 Score: 335.109 bits (858), Expect = 4.639e-111 Identity = 150/271 (55.35%), Postives = 199/271 (73.43%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
F C +C SL G +YI EE P+CI CY++ FANTC++CKE IG D ++L ++DRH+HE CF CF C SL D PF +DE L C++CY N+F+++C C K G RK EY G+ WHE CF+C C+QPIG++SFIP +Q C+PCYE+K A +C+ C + + +GGVTY+ PWHKECF CT C QLAG +FTS+D+KPYC C+G L+AKKC CTKPITGFGG K++SFE RHWH CF CS+C+T+LVG+GF+ + ++I+C C
Sbjct: 5 FDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRAC 275 HSP 2 Score: 148.673 bits (374), Expect = 5.743e-40 Identity = 87/277 (31.41%), Postives = 134/277 (48.38%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH-FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C CKE L Y YC Y C C ELI ++ + H+ H F C CD+SL + ++E C CY +F++ C C++ + S+ L + + WHE CF C C + R F ++++ +C CY++K A RC C K G YR + WH+ECF+C CK + + F +D++ C+ C+ N YA+KC+ CT+ I G V+++ WH CF C+ C+ L G F ++ C C +L
Sbjct: 5 FDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKG--GVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRACNSDL 279 HSP 3 Score: 120.939 bits (302), Expect = 4.661e-30 Identity = 73/245 (29.80%), Postives = 113/245 (46.12%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C ECK IG EL H +H CF C C L D + +L C Y + +C +C++ + Q H + F C +C + +I + +N +CI CYE+K A C CK+ + ++++D WH++CF C C T L + F ++DE +C +C+ N +A +C GCTK G + + + WH CF C C + K FIP ++ I+C C
Sbjct: 40 CDECKELIGHDCRELYYEDRH-------YHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSL--TKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRAC 275 HSP 4 Score: 89.3521 bits (220), Expect = 3.384e-19 Identity = 48/153 (31.37%), Postives = 76/153 (49.67%), Query Frame = 1
Query: 1291 ECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRG--GVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
E F C CK+ + + +I ++ C+PCY++ +A C +C E+I + Y+ +H+ CF C C LA FT +D++ C DCY F+ KC C K + G + + + G+ WH CF C+ C + R F+ N C C
Sbjct: 3 EPFDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTV--MPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPC 153
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl5 (four and a half LIM domains 5 [Source:NCBI gene;Acc:100144736]) HSP 1 Score: 328.176 bits (840), Expect = 2.476e-108 Identity = 145/272 (53.31%), Postives = 197/272 (72.43%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
F C +C SL G +Y LK++ P+CIKC+ + FAN CE+CK+ I C+SKDL++KD HWHE CF+C +C SLV++PFA KDE L C ECY +++++C GC G RK EY G WHE CF+C C++P+G K FIP++ +I C+PCYE ++A +C C + I +GG++++ WH+ECF CT+C K L G K TS+D+ PYC DC+ L+AKKC C KPITG GG K+ISFE R WHS+CFTC+KC+ +LVG F TNED+++CP CG
Sbjct: 6 FDCFHCKESLYGKKYTLKDDIPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKGGLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSCG 277 HSP 2 Score: 159.458 bits (402), Expect = 6.076e-44 Identity = 88/273 (32.23%), Postives = 137/273 (50.18%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI-FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C CKE L Y + YC + + C C + I N + D H F C CD+SL + K+E CI+CY T++++ C C+ I S+ + + +WHE CF C C + ++PF K+ ++C CY+ +FA +C C K G ++ +QWH ECF+C CK+ + + RD+ CV C++N YA+KC+ C + I G ++++ WH +CFTC C+K L G KF + +D C C
Sbjct: 6 FDCFHCKESLYGKKYTLKDDIPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKG--GLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276 HSP 3 Score: 122.094 bits (305), Expect = 2.051e-30 Identity = 66/219 (30.14%), Postives = 107/219 (48.86%), Query Frame = 1
Query: 736 WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVF--KAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
WH CF C +C LV+ + L C Y + +C C I +R M+ H F C++C + +I KE +C+ CYE +FAN C+ C++ I LSF+++ WH +CF C C +LV ++DES +C +C+DN +A +C C K + G + + +QWH +CF C +C + + + F + ++C C
Sbjct: 62 WHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPG--SRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAI--TKGGLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276 HSP 4 Score: 96.6709 bits (239), Expect = 1.325e-21 Identity = 57/200 (28.50%), Postives = 101/200 (50.50%), Query Frame = 1
Query: 601 SHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCD--SKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSEC 1194
+ D + +E + +CF C+ TI S ++++N S +WH CF C C+E + + + +K+YC Y ++ +C +C + I + +Q+ H F C +C +L G + ++E+P+C+ C++ +A C C + I +K +SF+DR WH CF C +C SLV F T ++ + C C
Sbjct: 83 AKDELLLCIECYSTEYSSKCFGCRATIMPG--SRKMEYNGS---NWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKGGLS-FQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334]) HSP 1 Score: 265.003 bits (676), Expect = 1.048e-83 Identity = 122/274 (44.53%), Postives = 169/274 (61.68%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFG-GCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C C L G +YI K+ + C+KC++ ANTC +C++ IG DSK+L +K+R+WH+ CF C +C+ L + F KD + C++C + + RC GC K + G R EY+G WHEECF C CKQ IG+ SF P+ + CV C+E K+A+ C KC I GG+TY+ PWH +CF C C K+LAG +FT+ +D YC DCY AKKC C PITGFG G +++EG WH CFTC KC+ NL + F+ + + + C DC +
Sbjct: 21 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAK 294 HSP 2 Score: 127.487 bits (319), Expect = 3.856e-32 Identity = 77/278 (27.70%), Postives = 128/278 (46.04%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI-FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG-----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C C+ L Y C + + + CA C + I + + ++ H N F C C + L ++I K+ C KC + + C C ++I +++ +K WHE+CF C C ++ F K ++C C++ KFA C C +G Y+ + WH +CF+C C + + + F + CV CY++ A+KC+ C I G V Y+G WH+ CFTC C+ LA +F +++ YC DC ++
Sbjct: 21 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSG--GITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 296 HSP 3 Score: 117.087 bits (292), Expect = 1.728e-28 Identity = 74/253 (29.25%), Postives = 116/253 (45.85%), Query Frame = 1
Query: 652 VQCFE--CKTTIGKSELSVRIDHNESGAHS--WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI----FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKV---FKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+CF+ C T + + +D E + WH CF C++C L + + NK+ CA+ + RC+ C + I N EY + H F C NC ++ + K + +C+ C+E KFA C +C I S ++++D+ WH CF C CH L + F ++ +C +CY + A +C GC F G Y G WHE CF C +C + K F+ ++Q+ C C
Sbjct: 45 VKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSA---WHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPI--TSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 292
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334]) HSP 1 Score: 264.618 bits (675), Expect = 1.229e-83 Identity = 122/274 (44.53%), Postives = 169/274 (61.68%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFG-GCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C C L G +YI K+ + C+KC++ ANTC +C++ IG DSK+L +K+R+WH+ CF C +C+ L + F KD + C++C + + RC GC K + G R EY+G WHEECF C CKQ IG+ SF P+ + CV C+E K+A+ C KC I GG+TY+ PWH +CF C C K+LAG +FT+ +D YC DCY AKKC C PITGFG G +++EG WH CFTC KC+ NL + F+ + + + C DC +
Sbjct: 16 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAK 289 HSP 2 Score: 127.102 bits (318), Expect = 4.033e-32 Identity = 77/278 (27.70%), Postives = 128/278 (46.04%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI-FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG-----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C C+ L Y C + + + CA C + I + + ++ H N F C C + L ++I K+ C KC + + C C ++I +++ +K WHE+CF C C ++ F K ++C C++ KFA C C +G Y+ + WH +CF+C C + + + F + CV CY++ A+KC+ C I G V Y+G WH+ CFTC C+ LA +F +++ YC DC ++
Sbjct: 16 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSG--GITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 291 HSP 3 Score: 117.087 bits (292), Expect = 1.412e-28 Identity = 74/253 (29.25%), Postives = 116/253 (45.85%), Query Frame = 1
Query: 652 VQCFE--CKTTIGKSELSVRIDHNESGAHS--WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI----FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKV---FKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+CF+ C T + + +D E + WH CF C++C L + + NK+ CA+ + RC+ C + I N EY + H F C NC ++ + K + +C+ C+E KFA C +C I S ++++D+ WH CF C CH L + F ++ +C +CY + A +C GC F G Y G WHE CF C +C + K F+ ++Q+ C C
Sbjct: 40 VKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSA---WHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPI--TSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 287
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762]) HSP 1 Score: 357.066 bits (915), Expect = 7.336e-120 Identity = 160/273 (58.61%), Postives = 204/273 (74.73%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YILKEENPHC+ C+E +ANTCE+C IGCD KDLS+KDRHWHE CF C C +SLVD+PFA K+E L C++CY N+++++C C K G RK EY+G WHE CF C C+QPIG KSFIP++ Q CVPCYE +YA +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++D+ PYC C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 7.615e-38 Identity = 83/273 (30.40%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y +C + E C C I + + H H F C C +SL + KEE C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F K+ FC CY+ ++A +C C K G YR + WH+ECF+C CK+ + + F RD+ C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 120.939 bits (302), Expect = 3.969e-30 Identity = 80/272 (29.41%), Postives = 121/272 (44.49%), Query Frame = 1
Query: 583 FLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
LK N H V E C EC T IG +LS + H WH CF+CS C LVD + +L C Y + +C C + I TR M+ H F C+ C + +I KE C+ CYE ++A C QCK+ I + ++++++ WH++CF C C L + F +DE +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 20 ILKEENPH-----CVACFEELYANTCEECGTPIGCDCKDLSYKDRH-------WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762]) HSP 1 Score: 357.066 bits (915), Expect = 7.336e-120 Identity = 160/273 (58.61%), Postives = 204/273 (74.73%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YILKEENPHC+ C+E +ANTCE+C IGCD KDLS+KDRHWHE CF C C +SLVD+PFA K+E L C++CY N+++++C C K G RK EY+G WHE CF C C+QPIG KSFIP++ Q CVPCYE +YA +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++D+ PYC C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 7.615e-38 Identity = 83/273 (30.40%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y +C + E C C I + + H H F C C +SL + KEE C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F K+ FC CY+ ++A +C C K G YR + WH+ECF+C CK+ + + F RD+ C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 120.939 bits (302), Expect = 3.969e-30 Identity = 80/272 (29.41%), Postives = 121/272 (44.49%), Query Frame = 1
Query: 583 FLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
LK N H V E C EC T IG +LS + H WH CF+CS C LVD + +L C Y + +C C + I TR M+ H F C+ C + +I KE C+ CYE ++A C QCK+ I + ++++++ WH++CF C C L + F +DE +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 20 ILKEENPH-----CVACFEELYANTCEECGTPIGCDCKDLSYKDRH-------WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl3 (four and a half LIM domains 3 [Source:MGI Symbol;Acc:MGI:1341092]) HSP 1 Score: 319.701 bits (818), Expect = 3.719e-105 Identity = 137/273 (50.18%), Postives = 191/273 (69.96%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C C+ SL G +YI + P+C+ CY+ FANTC +C++ IG DS++L ++DRH+HE CF C C SL D PF +D L C+ECY F+++C C + G RK EY G+ WHE CFLC C+QP+G++SF+P CVPCYENK+A +C++C++ + +GGVTY+ PWH+EC CT C LAG +FTS+DD PYC C+GELFA KC+ C +PITG GG K++SFE RHWH CF+C++C+T+LVG+GF+ + D ++C C +
Sbjct: 5 FDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQ 277 HSP 2 Score: 154.836 bits (390), Expect = 2.731e-42 Identity = 89/273 (32.60%), Postives = 123/273 (45.05%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C++C E L Y + YC Y CA C +LI ++ + H H F C C SL + ++ C +CY T F++ C C E + S+ L + + WHE CF C C L R F + +C CY+NKFA RC C+K G YR + WH EC +C CK P+ + F RD CV C+ +A KCS C I G V+++ WH CF+C C+ L G F D+ C C
Sbjct: 5 FDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275 HSP 3 Score: 114.005 bits (284), Expect = 1.038e-27 Identity = 61/245 (24.90%), Postives = 105/245 (42.86%), Query Frame = 1
Query: 658 CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYE 1383
C EC+ IG + + +H CF C C+ L D + ++L C Y +C+ C E + Q H + F C C+ L ++ + +C+ CYE KFA C +C + + ++++D+ WH +C C C T L + F ++D+ +C C+ FA +C C + G + + + WH CF C C + + F+P Q++C C +
Sbjct: 40 CAECQQLIGHDSRELFYEDRH-----FHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTL--TQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQ 277
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl5 (four and a half LIM domains 5 [Source:MGI Symbol;Acc:MGI:1913192]) HSP 1 Score: 308.145 bits (788), Expect = 1.383e-100 Identity = 142/274 (51.82%), Postives = 188/274 (68.61%), Query Frame = 1
Query: 925 NHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
+ F C+ C +SL G +Y+LK++N +CI CY+ F+N CEQCKE I DSKDL +K+RHWHE CF C +CH SLV++PF KD+ L C++CY N+ +++C C + G RK E++G WHE CF+C C+QPIG K I ++ CVPC+E ++A C+ C +VI GG+T++ WHKECF C+ C K+L F SKDD P+C DCY L+AKKC CTKPITG G KFI F+ R WHSECF C KC+ +LVG GFLT+ I+C CG
Sbjct: 4 SQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCG 277 HSP 2 Score: 144.821 bits (364), Expect = 1.351e-38 Identity = 84/273 (30.77%), Postives = 132/273 (48.35%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIR--RGG--VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C L+ Y + LYC Y C C E I ++ H H F C C +SL ++ K++ C CY + ++ C CK I S+ + FK +WHE CF C C + +P +K+ +C C++ +FA C+ C KV +G +R + WH+ECFLC C++ + ++F+ +D C+ CY + YA+KC+ CT+ I RG + ++ WH ECF C C+ L G F + + + C C
Sbjct: 6 FDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSG--GITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276 HSP 3 Score: 116.701 bits (291), Expect = 1.119e-28 Identity = 65/217 (29.95%), Postives = 108/217 (49.77%), Query Frame = 1
Query: 736 WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
WH CF C++C LV+ + ++L C Y + +C C I + H F C++C + I KE +C+ C+E +FA+ C CK+ I S ++F+D+ WH++CF C C L + F +KD+ FC +CY++ +A +C CTK G + ++ +QWH ECF C +C + + F+ + +I+C C
Sbjct: 62 WHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVI--TSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl5 (four and a half LIM domains 5 [Source:MGI Symbol;Acc:MGI:1913192]) HSP 1 Score: 308.145 bits (788), Expect = 1.383e-100 Identity = 142/274 (51.82%), Postives = 188/274 (68.61%), Query Frame = 1
Query: 925 NHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
+ F C+ C +SL G +Y+LK++N +CI CY+ F+N CEQCKE I DSKDL +K+RHWHE CF C +CH SLV++PF KD+ L C++CY N+ +++C C + G RK E++G WHE CF+C C+QPIG K I ++ CVPC+E ++A C+ C +VI GG+T++ WHKECF C+ C K+L F SKDD P+C DCY L+AKKC CTKPITG G KFI F+ R WHSECF C KC+ +LVG GFLT+ I+C CG
Sbjct: 4 SQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCG 277 HSP 2 Score: 144.821 bits (364), Expect = 1.351e-38 Identity = 84/273 (30.77%), Postives = 132/273 (48.35%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIR--RGG--VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C L+ Y + LYC Y C C E I ++ H H F C C +SL ++ K++ C CY + ++ C CK I S+ + FK +WHE CF C C + +P +K+ +C C++ +FA C+ C KV +G +R + WH+ECFLC C++ + ++F+ +D C+ CY + YA+KC+ CT+ I RG + ++ WH ECF C C+ L G F + + + C C
Sbjct: 6 FDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSG--GITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276 HSP 3 Score: 116.701 bits (291), Expect = 1.119e-28 Identity = 65/217 (29.95%), Postives = 108/217 (49.77%), Query Frame = 1
Query: 736 WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA--GMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
WH CF C++C LV+ + ++L C Y + +C C I + H F C++C + I KE +C+ C+E +FA+ C CK+ I S ++F+D+ WH++CF C C L + F +KD+ FC +CY++ +A +C CTK G + ++ +QWH ECF C +C + + F+ + +I+C C
Sbjct: 62 WHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVI--TSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O70433|FHL2_MOUSE (Four and a half LIM domains protein 2 OS=Mus musculus OX=10090 GN=Fhl2 PE=1 SV=1) HSP 1 Score: 357.066 bits (915), Expect = 5.131e-119 Identity = 160/273 (58.61%), Postives = 204/273 (74.73%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YILKEENPHC+ C+E +ANTCE+C IGCD KDLS+KDRHWHE CF C C +SLVD+PFA K+E L C++CY N+++++C C K G RK EY+G WHE CF C C+QPIG KSFIP++ Q CVPCYE +YA +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++D+ PYC C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 5.327e-37 Identity = 83/273 (30.40%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y +C + E C C I + + H H F C C +SL + KEE C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F K+ FC CY+ ++A +C C K G YR + WH+ECF+C CK+ + + F RD+ C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 120.939 bits (302), Expect = 2.776e-29 Identity = 80/272 (29.41%), Postives = 121/272 (44.49%), Query Frame = 1
Query: 583 FLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
LK N H V E C EC T IG +LS + H WH CF+CS C LVD + +L C Y + +C C + I TR M+ H F C+ C + +I KE C+ CYE ++A C QCK+ I + ++++++ WH++CF C C L + F +DE +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 20 ILKEENPH-----CVACFEELYANTCEECGTPIGCDCKDLSYKDRH-------WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O35115|FHL2_RAT (Four and a half LIM domains protein 2 OS=Rattus norvegicus OX=10116 GN=Fhl2 PE=1 SV=1) HSP 1 Score: 356.681 bits (914), Expect = 7.743e-119 Identity = 159/273 (58.24%), Postives = 204/273 (74.73%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YILKEENPHC+ C+E +ANTCE+C IGCD KDLS+KDRHWHE CF C C +SLVD+PFA K+E L C++CY N+++++C C K G RK EY+G WHE CF C C+QPIG KSFIP++ Q CVPCYE +YA +C +C + I GGVTY+ PWH+ECF CT C KQL+G +FT++D+ PYC C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 4.493e-37 Identity = 83/273 (30.40%), Postives = 127/273 (46.52%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y +C + E C C I + + H H F C C +SL + KEE C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F K+ FC CY+ ++A +C C K G YR + WH ECF+C CK+ + + F RD+ C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 122.094 bits (305), Expect = 8.435e-30 Identity = 81/272 (29.78%), Postives = 120/272 (44.12%), Query Frame = 1
Query: 583 FLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
LK N H V E C EC T IG +LS + H WH CF+CS C LVD + +L C Y + +C C + I TR M+ H F C+ C + +I KE C+ CYE ++A C QCK+ I + ++++D+ WH +CF C C L + F +DE +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 20 ILKEENPH-----CVACFEELYANTCEECGTPIGCDCKDLSYKDRH-------WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q14192|FHL2_HUMAN (Four and a half LIM domains protein 2 OS=Homo sapiens OX=9606 GN=FHL2 PE=1 SV=3) HSP 1 Score: 356.295 bits (913), Expect = 1.260e-118 Identity = 158/273 (57.88%), Postives = 207/273 (75.82%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C+ SL G +YIL+EE+P+C+ C+ET FANTCE+C + IGCD KDLS+KDRHWHE CF C +C SLVD+PFA K++ L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIG KSFIP+D Q CVPCYE ++A +C +C + I GGVTY+ PWHKECF CT C KQL+G +FT++DD YC +C+ +L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 142.51 bits (358), Expect = 4.316e-37 Identity = 83/273 (30.40%), Postives = 126/273 (46.15%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C E L Y YC + C C + I + + H H F C C NSL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ + A +C C K G YR + WH+ECF+C C++ + + F RD C+ C+ + YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 1.540e-27 Identity = 72/247 (29.15%), Postives = 116/247 (46.96%), Query Frame = 1
Query: 658 CFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C EC IG +LS + H WH ACF+CS+C+ LVD + ++L C Y + +C C + I TR M+ H F C C + +I K+ C+ CYE + A C QCK+ I + ++++++ WH++CF C C L + F +D+ +C C+ + +A +C GCT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 40 CEECGKPIGCDCKDLSYKDRH-------WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q2KI95|FHL2_BOVIN (Four and a half LIM domains protein 2 OS=Bos taurus OX=9913 GN=FHL2 PE=2 SV=1) HSP 1 Score: 344.354 bits (882), Expect = 4.809e-114 Identity = 152/273 (55.68%), Postives = 201/273 (73.63%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C +C++SL G +Y+L+EE P+C+ C+E FA+TCE+C + IGCD KDLS+KDRHWHE CF C C SLVD+PFA K++ L C++CY ++++RC C K G RK EY+G WHE CF+C C+QPIG KSFIP+D + CVPCYE +YA +C +C + I GGVTY+ PWH+ECF CT C K L+G +FTS+D+ YC C+ +L+AKKC C PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CPDCG+
Sbjct: 5 FDCHHCEDSLFGRKYVLREEQPYCVACFEALFASTCEECGKLIGCDCKDLSYKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 148.673 bits (374), Expect = 3.265e-39 Identity = 83/273 (30.40%), Postives = 129/273 (47.25%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C++ L Y + YC + C C +LI + + H H F C C SL + KE+ C CY ++++ C++CK+ I ++ + +K WHE CF C C + + F KD FC CY+ ++A +C C K G YR + WH ECF+C CK+P+ + F RD+ C+ C+ + YA+KC+ C I G ++++ WH +CF C C+ L G F ++ D C DC
Sbjct: 5 FDCHHCEDSLFGRKYVLREEQPYCVACFEALFASTCEECGKLIGCDCKDLSYKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTG--GVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 117.087 bits (292), Expect = 4.998e-28 Identity = 77/268 (28.73%), Postives = 122/268 (45.52%), Query Frame = 1
Query: 595 RNSHDLSIAHVEAITEFQGVQCFECKTTIGKS--ELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
R +A EA+ C EC IG +LS + H WH ACF+CS C+ LVD + ++L C Y ++ RC C + I TR M+ H F C C + +I K+ C+ CYE ++A C QCK+ I + ++++++ WH +CF C C L + F ++DE +C C+ + +A +C GC G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 22 REEQPYCVACFEALF---ASTCEECGKLIGCDCKDLSYKDRH-------WHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPI--TTGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q3ZBI6|FHL3_BOVIN (Four and a half LIM domains protein 3 OS=Bos taurus OX=9913 GN=FHL3 PE=2 SV=1) HSP 1 Score: 317.39 bits (812), Expect = 1.954e-103 Identity = 135/273 (49.45%), Postives = 192/273 (70.33%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C C SL G +YI ++ P+C+ CY+ FANTC +C++ IG DS++L ++DRH+HE CF C C SL D PF +D L C++CY + F+++C C + G RK EY G+ WHE CFLC C+QP+G++SF+P CVPCYENK+A +C++C++ + +GGVTY+ PWH+EC CT C LAG +FTS++D PYC C+GELFA KC+ C +PITG GG K++SFE RHWH CF+C++C+T+LVG+GF+ + D ++C C +
Sbjct: 5 FDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQ 277 HSP 2 Score: 150.214 bits (378), Expect = 7.526e-40 Identity = 86/273 (31.50%), Postives = 122/273 (44.69%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C++C E L Y + YC Y CA C +LI ++ + H H F C C SL + ++ C CY + F++ C C E + S+ L + + WHE CF C C L R F + +C CY+NKFA RC C+K G YR + WH EC +C C+ P+ + F R+ CV C+ +A KCS C I G V+++ WH CF+C C+ L G F D+ C C
Sbjct: 5 FDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275 HSP 3 Score: 112.464 bits (280), Expect = 2.293e-26 Identity = 60/245 (24.49%), Postives = 105/245 (42.86%), Query Frame = 1
Query: 658 CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYE 1383
C EC+ IG + + +H CF C C+ L D + ++L C Y +C+ C E + Q H + F C C+ L ++ + +C+ CYE KFA C +C + + ++++D+ WH +C C C T L + F ++++ +C C+ FA +C C + G + + + WH CF C C + + F+P Q++C C +
Sbjct: 40 CAECQQLIGHDSRELFYEDRH-----FHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTL--TQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQ 277
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A068Y1Z9 (Four and A half lim domains OS=Echinococcus multilocularis OX=6211 PE=4 SV=1) HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0 Identity = 354/553 (64.01%), Postives = 410/553 (74.14%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRR-LDKFSHLDTNV-----------ERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRI--------DHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MAL+QDIG PC+ C C GF+LH WR TCTAC+CSYD H V D + L E++LSD + + Y AQ + GL+WLP+G++ EVE+FL+SLP+S P G + R Q+P QDR+ S N+ E +EA +F +RN D I VE + V C EC IG E VRI + S +WHP CF CS C E LVD++Y W K YC RHYG+ IRPRCATCD LIF+EEYTRAMDQEHH HFAC++CD SLTG RYIL++E PHC+ CYE KFANTCEQCKEKIGCDSKDLSFK+RHWHEKCF+C C+TSL DRPFATKD+ L+CS+CYD +FAARCDGC VFKAGMRKYEYRG+QWHEECF+C+ECKQPIGAKSFIPRD Q++CVPCYE KYAQ+C+KC+EVIRRGGVTYKG PWHKECFTCT+C KQLAG+KFTSKD++PYCADCYG+LFAKKCTKC KPITGFGGCKFISFE RHWHSECF CSKC NLVGRGFLTNED IMC DCGR
Sbjct: 1 MALQQDIGKPCLTCGSECPGFKLHPWRATCTACHCSYDKHAVTDPSKDSYLHELELSDTTVLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLTSLPSSEIPRGEAYVEMRTRRIR-HQVPPQDRKPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFREFCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: U6J6Y3 (Four and a half LIM domains protein 2 OS=Echinococcus granulosus OX=6210 GN=EGR_09187 PE=4 SV=1) HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0 Identity = 354/553 (64.01%), Postives = 410/553 (74.14%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRR-LDKFSHLDTNV-----------ERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRI--------DHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MAL+QDIG PC+ C C GF+LH WR TCTAC+CSYD H V D + L E++LSD + + Y AQ + GL+WLP+G++ EVE+FL+SLP+S P G + R Q+P QDR+ S N+ E +EA +F +RN D I VE + V C EC IG E VRI + S +WHP CF CS C E LVD++Y W K YC RHYG+ IRPRCATCD LIF+EEYTRAMDQEHH HFAC++CD SLTG RYIL++E PHC+ CYE KFANTCEQCKEKIGCDSKDLSFK+RHWHEKCF+C C+TSL DRPFATKD+ L+CS+CYD +FAARCDGC VFKAGMRKYEYRG+QWHEECF+C+ECKQPIGAKSFIPRD Q++CVPCYE KYAQ+C+KC+EVIRRGGVTYKG PWHKECFTCT+C KQLAG+KFTSKD++PYCADCYG+LFAKKCTKC KPITGFGGCKFISFE RHWHSECF CSKC NLVGRGFLTNED IMC DCGR
Sbjct: 1 MALQQDIGKPCLTCASECPGFKLHPWRATCTACHCSYDKHAVTDPSKDSYLHELELSDTTVLKAYDVAQTVAREHGLHWLPVGIQSSEVEAFLTSLPSSEIPRGEAYVEMRTRRIR-HQVPPQDRKPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFREFCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3X1E4 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7003 PE=4 SV=1) HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0 Identity = 352/553 (63.65%), Postives = 409/553 (73.96%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRR-LDKFSHLDTNV-----------ERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRI--------DHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MAL+QD+G PC+ C C GF+LH WR TCT C+CSYD H V D + L E++LSD + + Y AQ K GL+WLP+G++ EVE+FL+SLP+S P G + R Q+P QDR+ S N+ E +EA +F +RN D I VE ++ + C EC IG E VR+ + S A +WHP CF CS C E LVD +Y W K YC RHYG+ IRPRCATCD LIF+EEYTRAMDQEHH HFAC++CD SLTG RYIL++E PHC+ CYE KFANTCEQCKEKIGCDSKDLSFK+RHWHEKCF+C C+TSL DRPFATKD+ L+CS+CYD +FAARCDGC VFKAGMRKYEYRG+QWHEECF+C+ECKQPIGAKSFIPRD Q++CVPCYE KYAQ+C+KC EVIRRGGVTYKG PWHKECFTCT+C KQLAG+KFTSKD++PYCADCYGELFAKKCTKC KPITGFGGCKFISFE RHWHSECF CSKC NLVGRGFLTN+D IMC DCGR
Sbjct: 1 MALQQDVGKPCLTCGSECSGFKLHPWRATCTGCHCSYDKHAVTDPSKDSYLHELELSDVTVLKAYDVAQAIAKEHGLHWLPVGIQSAEVEAFLTSLPSSEIPRGEVYAEMRFRRIR-HQVPPQDRKPASSLSTAKLNLPPSTAPANLEGESREATRFQNSRNRRDFGIGRVERVSAKATIVCAECSEHIGFREFCVRVRPEHRLSDSSDTSYAPAWHPGCFRCSNCSEHLVDLVYGWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCAEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNDDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3W1E0 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS3546 PE=4 SV=1) HSP 1 Score: 722.235 bits (1863), Expect = 0.000e+0 Identity = 350/553 (63.29%), Postives = 409/553 (73.96%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRR-LDKFSHLDTNV-----------ERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRI--------DHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MAL+QD G PC+ C C GF+LH WR TCT C+CSY+ H V D + L E+++SD + + Y AQ + GL+WLP+G++ EVE+FL+SLP+S P G + R Q+P QDR+ S N+ E +EA +F +RN D I VE + V C EC IG E VRI + S A +WHP CF CS C E LVD++Y W K YC RHYG+ IRPRCATCD LIF+EEYTRAMDQEHH HFAC++CD SLTG RYIL++E PHC+ CYE KFANTCEQCKEKIGCDSKDLSFK+RHWHEKCF+C C+TSL DRPFATKD+ L+CS+CYD +FAARCDGC VFKAGMRKYEYRG+QWHEECF+C+ECKQPIGAKSFIPRD Q++CVPCYE KYAQ+C+KC+EVIRRGGVTYKG PWHKECFTCT+C KQLAG+KFTSKD++PYCADCYG+LFAKKCTKC KPITGFGGCKFISFE RHWHSECF CSKC NLVGRGFLTN+D IMC DCGR
Sbjct: 1 MALQQDTGKPCLTCGSECPGFKLHPWRATCTNCHCSYEKHAVTDPSKDSYLHELEVSDTTLLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLTSLPSSEIPRGEAYAEMRFRRIR-HQVPPQDRKPASSLSTAKLNLPPSNAPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFREFCVRIRPEHRLSDSSDNSYAPAWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCNCNLVGRGFLTNDDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A4S2L6K0 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009563 PE=4 SV=1) HSP 1 Score: 718.383 bits (1853), Expect = 0.000e+0 Identity = 357/564 (63.30%), Postives = 415/564 (73.58%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRR----------------LDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRI-------------DHNESG--AHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MA QD C++C C GF H WR TCT C C+YD HDV + + +LDE++L + Y+ Y+EAQK K +WLPIGL P EV+SFL+SLP+S P G D+ R RRLRKQIP QDRR L SH + E +EA +F +RN D I VE +++ G C +C TIG + +R+ NE+ A +WH CF C+ C E LVDY Y W + YC RHYG+ IRPRCATCD LIF+EEYTRAMDQEHH HFAC++CD SLTG RYIL+++ PHC+ CYE KFANTCEQCKEKIGCDSKDLSFK+RHWHEKCF+C C TSL DRPFATK+E L+CS+CYD +FAARCDGC VFKAGMRKYEYRG+QWHEECFLC+ECKQPIGAKSFIPR+ Q++CVPCYE KYAQ+C+KC+EVIRRGGVTYKG PWHKECFTCTNCTKQLAG+KFTSKD++PYCADCYGELFAKKCTKCTKPITGFGGCKFISFE RHWHSECF C KC NLVGRGFLT++D IMC DCGR
Sbjct: 1 MAYDQDTAKACLKCGPSCSGFAKHAWRMTCTKCRCAYDEHDVSEFSTQMLLDELELGESPLYKMYVEAQKLAKQCNTHWLPIGLHPAEVDSFLNSLPSSEVPRGD-AADRLRRRRLRKQIPPQDRRPAAAVEEAWVHRTSSQLPDASH-SLDSELREANRFKNSRNRRDFGIGMVEHMSQSAGQACADCLGTIGFGDFCIRVRPEHRSLEAMTNSPSNEASDLAPAWHVGCFRCATCSEHLVDYCYAWSNGRPYCLRHYGQLIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDASLTGQRYILRDDEPHCLGCYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACATSLADRPFATKEEQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFLCVECKQPIGAKSFIPRENQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTNCTKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEDRHWHSECFLCGKCNCNLVGRGFLTSDDMIMCSDCGR 562
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:103031012]) HSP 1 Score: 354.369 bits (908), Expect = 7.333e-119 Identity = 156/273 (57.14%), Postives = 206/273 (75.46%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L+EENP+C+KCYE+ ++NTCE+CK+ IGC+S+DLS+KDRHWHE+CF CF+C SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C C + I GGVTY PWHK+CF CT C +QL+G +FTS+DD YC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 145.206 bits (365), Expect = 5.120e-39 Identity = 86/273 (31.50%), Postives = 127/273 (46.52%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y YC + Y C C + I N D+ H F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD + +C CY+ +FA +C C K G Y + WH++CFLC CKQ + + F RD C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 119.398 bits (298), Expect = 1.004e-29 Identity = 75/258 (29.07%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF+C +CK LVD + +L C Y + +C C + I +R M+ + + H F CK C + +I K+ N +C+ CYE +FA C CK+ I + +++ D+ WH+ CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNSRDLSYKDRH-------WHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:103023824]) HSP 1 Score: 339.732 bits (870), Expect = 4.693e-113 Identity = 154/271 (56.83%), Postives = 197/271 (72.69%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
F C NC SL G +YI E+NP+CI CY++ FANTC++CKE IG D+++L ++DRH+HE CF CF C SL D PF ++D++L C++CY N+F+++C C K G RK EY G WHE CF+C C+QPIG+KSFIP CVPCYENK+A +C++C +V+ +GGVTY+ PWHKECF CT C QLAG FTS+DD PYC C+G L+AKKC C+KPITGFGG K+ISFE R WH CFTCS+C+ +LVG GF D I+C DC
Sbjct: 5 FDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDC 275 HSP 2 Score: 153.295 bits (386), Expect = 6.501e-42 Identity = 89/277 (32.13%), Postives = 131/277 (47.29%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH-FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C CKE L Y + YC Y C C ELI ++ + H+ H F C CD SL + +++ C CY +F++ C C + + ++ L + WHE CF C C + + F + +C CY+NKFA RC C +V G YR + WH+ECFLC CK + + F RD C+ C+ N YA+KC C++ I G ++++ WH+ CFTC+ C+ L G F + D+ C DC L
Sbjct: 5 FDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKG--GVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCNSNL 279 HSP 3 Score: 119.013 bits (297), Expect = 1.525e-29 Identity = 68/248 (27.42%), Postives = 115/248 (46.37%), Query Frame = 1
Query: 658 CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYEN 1386
C ECK IG + + +H CF C C L D + + L C Y + +C CD+ + TR ++ H F C +C+ + +I +++ +C+ CYE KFA C +CK+ + ++++D WH++CF C C L + F ++D+S +C +C+ N +A +C+ C+K G + + +QWH+ CF C C + F P +I+C C N
Sbjct: 40 CDECKELIGHDARELFYEDRH-----YHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPG--TRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLA--KGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCNSN 278
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 297.745 bits (761), Expect = 3.995e-97 Identity = 139/273 (50.92%), Postives = 192/273 (70.33%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L+EENP+C+KCYE+ ++NTCE+CK+ I C+S+DLS+KDRHWH+ CF CF+C+ SLVD+PF+TK+E L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPC+E ++A +C +C +V Y T +CF CT C +QL+G +FTS+DD PYC +C+ L+AKKC+ CT PI+G GG K+ISFE R WH++CF C KC+ D+I+CPDCG+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKV----HTVYNST----DCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKCSI-----------DDILCPDCGK 258 HSP 2 Score: 118.242 bits (295), Expect = 1.582e-29 Identity = 76/253 (30.04%), Postives = 114/253 (45.06%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCT 1494
++C CKE L Y YC + Y C C + I N D+ H + F C C+ SL + K E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD + +C C++ +FA +C C KV +CFLC CK+ + + F RD C+ C+ N YA+KCS CT I G ++++ WH +CF C C+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNS----------TDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKCS 247 HSP 3 Score: 83.1889 bits (204), Expect = 1.978e-17 Identity = 63/237 (26.58%), Postives = 100/237 (42.19%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTI--GKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLEC 1314
V+ C ECK I +LS + H WH CF+C +C LVD + +L C Y + +C C + I +R M+ + + H F C+ C + +I K+ +C+ C+E +FA C QCK+ + CF C C L + F ++D+ +C C+ N +A +C CT G + + +QWH +CF C +C
Sbjct: 29 VKCYESLYSNTCEECKKPICCNSRDLSYKDRH-------WHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKK----------VHTVYNSTDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKC 246
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1b (four and a half LIM domains protein 1 [Source:NCBI gene;Acc:103032814]) HSP 1 Score: 259.996 bits (663), Expect = 3.199e-82 Identity = 120/276 (43.48%), Postives = 172/276 (62.32%), Query Frame = 1
Query: 925 NHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFG-GCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ F C C + L G +YI K++ P C++C+E ANTC +C+ IG DSK+L K R+WHE CF C++C+ +L F+TKD+ + C +C + A RC C K AG EY+G +HEECF C +CK+PI ++SF+ ++ I C C+E K+A++C+ C + I GGV Y+ PWH CF C +C K LAG +FTS +D+ YC DCY AKKC+ C PITGFG +++EG WH CF C KC+ NL + F++ ++ C DC +
Sbjct: 3 DRFDCFYCRDHLGGKKYIKKDDRPVCVRCFEKFCANTCTECRRPIGTDSKELHHKGRYWHEDCFRCYKCYKNLAKESFSTKDDRILCGKCSSREDAPRCHTCYKPILAGTENVEYKGNTFHEECFTCYQCKKPISSQSFLTKNDNIYCTSCHEKKFAKQCAGCKKAITSGGVNYQDQPWHSACFVCASCQKPLAGTRFTSHEDRVYCVDCYKNSVAKKCSACQNPITGFGKATNVVNYEGGTWHDYCFNCRKCSLNLSDKRFVSKNGDVYCSDCSK 278 HSP 2 Score: 142.124 bits (357), Expect = 8.198e-38 Identity = 81/274 (29.56%), Postives = 135/274 (49.27%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG-----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C C++ L Y ++ C R + + C C I + + H + F C C +L + K++ C KC + A C C + I ++++ +K +HE+CF C++C + + F TK+++++C+ C++ KFA +C GC K +G Y+ + WH CF+C C++P+ F + ++ CV CY+N A+KCS C I G V Y+G WH CF C C+ L+ +F SK+ YC+DC
Sbjct: 5 FDCFYCRDHLGGKKYIKKDDRPVCVRCFEKFCANTCTECRRPIGTDSKELHHKGRYWHEDCFRCYKCYKNLAKESFSTKDDRILCGKCSSREDAPRCHTCYKPILAGTENVEYKGNTFHEECFTCYQCKKPISSQSFLTKNDNIYCTSCHEKKFAKQCAGCKKAITSG--GVNYQDQPWHSACFVCASCQKPLAGTRFTSHEDRVYCVDCYKNSVAKKCSACQNPITGFGKATNVVNYEGGTWHDYCFNCRKCSLNLSDKRFVSKNGDVYCSDC 276 HSP 3 Score: 115.931 bits (289), Expect = 1.558e-28 Identity = 70/255 (27.45%), Postives = 109/255 (42.75%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKV---FKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V +F C EC+ IG S + H WH CF C +C + L + +++ C + + PRC TC + I E H F C C ++ ++ K +N +C C+E KFA C CK+ I S ++++D+ WH CF C C L F + ++ ++C +CY N A +C C F Y G WH+ CF C +C + K F+ ++ + C C
Sbjct: 29 VRCFEKFCANTCTECRRPIGTD--SKELHHK---GRYWHEDCFRCYKCYKNLAKESFSTKDDRILCGKCSSREDAPRCHTCYKPILAGTENVEYKGNTFHEECFTCYQCKKPISSQSFLTKNDNIYCTSCHEKKFAKQCAGCKKAI--TSGGVNYQDQPWHSACFVCASCQKPLAGTRFTSHEDRVYCVDCYKNSVAKKCSACQNPITGFGKATNVVNYEGGTWHDYCFNCRKCSLNLSDKRFVSKNGDVYCSDC 276
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707]) HSP 1 Score: 259.996 bits (663), Expect = 6.985e-82 Identity = 124/277 (44.77%), Postives = 173/277 (62.45%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDES-LFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFG-GCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR*L 1755
F C C ++L G +Y+ K++ C++C+E ANTC +C+ IG D+K+L+ K+RHWHE CF C +C+ L + FA +D+ + C +C + +ARC GC KV G + EY+ K WHEECF C ECKQPI + SF+ + C PC+E K+A+ C++C E I G+TY+ PWH ECF C C K LAG +FT+ +D YC DCY AKKC+ C PITGFG G +++E WH CF C KC+ +L + F+ N +NI C DC + L
Sbjct: 21 FDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 297 HSP 2 Score: 132.494 bits (332), Expect = 3.057e-34 Identity = 84/279 (30.11%), Postives = 126/279 (45.16%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENP-HCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG-----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C C++ L Y +K C R + + CA C I + H H + F C C L + +++ C KC + + C+ C + I K++ +K + WHE+CF CFEC + F TK + +C+ C++ KFA C C + A Y+ K WH ECF+C C++P+ F + CV CY+ A+KCS C I G V Y+ WH+ CF C C+ LA +F + YC DC +L
Sbjct: 21 FDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITAN--GITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 297 HSP 3 Score: 107.842 bits (268), Expect = 1.537e-25 Identity = 71/254 (27.95%), Postives = 112/254 (44.09%), Query Frame = 1
Query: 652 VQCFE--CKTTIGKSELSVRIDHNESGAHS--WHPACFNCSECKELLVDYIYCWHLN-KLYCARHYGEKIRPRCATCDELIF----NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKV---FKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+CFE C T + + +D E + WH CF C++C + L + + + K+ C + + RC C ++I N EY + H F C C + ++ K ++ +C C+E KFA C +CKE I + ++++D+ WH +CF C C L F ++ +C +CY A +C GC F G Y WHE CF C +C + K F+ + I C C
Sbjct: 45 VRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKV---WHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPI--TANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 293 HSP 4 Score: 85.8853 bits (211), Expect = 4.360e-18 Identity = 53/185 (28.65%), Postives = 83/185 (44.86%), Query Frame = 1
Query: 649 GVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKI---GCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSEC 1194
+C C I + +V H WH CF C ECK+ + + + YC + +K CA C E I T D+ H F C+ C L G R+ E+ +C+ CY+T A C C+ I G + +++++ WHE CF C +C SL + F E+++C++C
Sbjct: 115 SARCQGCYKVIMPGDKNVEYKHK-----VWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGIT-YQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 293
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 205.297 bits (521), Expect = 3.658e-63 Identity = 91/170 (53.53%), Postives = 123/170 (72.35%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK-AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG 1437
+ C C SL G +Y+L+EENP+C++CYET + + CE CK+ IGCDSKDLS+KDRHWHE CF+C C SL D+ F+ +DE L C+EC+ N ++A+C G +K+F G RK E+RG WHE CF C C+QPIG+KSF+PR++ CVPCYE ++AQ C+ C +V GG
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGG 182 HSP 2 Score: 110.923 bits (276), Expect = 2.348e-28 Identity = 50/171 (29.24%), Postives = 92/171 (53.80%), Query Frame = 1
Query: 1114 FECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG---VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGG 1617
++C C SL+ + + ++E+ +C CY+ + C+ C + + Y+ + WHE CF C C++ + K+F RD++++C C+ N Y+ KC+ +++ G + ++G+ WH+ CF C C + + F ++ YC CY + FA+ CT C K + G GG
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKK-VGGEGG 182 HSP 3 Score: 89.7373 bits (221), Expect = 5.020e-21 Identity = 46/151 (30.46%), Postives = 75/151 (49.67%), Query Frame = 1
Query: 1297 FLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIR--RGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
+ C CK+ + K ++ R++ CV CYE Y C C + I ++YK WH+ CF C C + LA F+++D+K C +C+ ++ KC +K + G K + F G WH CF C++C + + F+ E + C C
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRK-MEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 162 HSP 4 Score: 71.2478 bits (173), Expect = 1.403e-14 Identity = 43/169 (25.44%), Postives = 71/169 (42.01%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIG-CDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAG 1251
++C CKE L+ Y YC R Y C +C + I + + H H F C C+ SL + ++E C +C+ ++ C + ++ + F+ WHE CF C C + + F +++ +C CY+ +FA C C KV G
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 181 HSP 5 Score: 60.077 bits (144), Expect = 8.289e-11 Identity = 41/140 (29.29%), Postives = 61/140 (43.57%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIG 1062
C CK IG +LS + H WH CF C+ C+ L D + KL C + +CA +L F+ TR M+ H F C C + ++ +E++ +C+ CYE +FA C CK+ G
Sbjct: 48 CESCKQPIGCDSKDLSYKDRH-------WHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKL-FSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGG 179
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 198.364 bits (503), Expect = 1.787e-60 Identity = 89/171 (52.05%), Postives = 119/171 (69.59%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVT 1443
+ C C SL G +Y+L+EENP+C++CYET + + CE CK+ IGCDSKDLS+KDRHWHE CF+C C SL D+ F+ +DE L C+EC+ N ++A+C G RK E+RG WHE CF C C+QPIG+KSF+PR++ CVPCYE ++AQ C+ C +V GG T
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAA--NPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGGPT 174 HSP 2 Score: 111.694 bits (278), Expect = 1.283e-28 Identity = 50/173 (28.90%), Postives = 91/173 (52.60%), Query Frame = 1
Query: 1114 FECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFIS 1632
++C C SL+ + + ++E+ +C CY+ + C+ C + + Y+ + WHE CF C C++ + K+F RD++++C C+ N Y+ KC+ + + ++G+ WH+ CF C C + + F ++ YC CY + FA+ CT C K + G GG +S
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKK-VGGEGGPTRLS 177 HSP 3 Score: 89.7373 bits (221), Expect = 5.465e-21 Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 1
Query: 1303 CLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIR--RGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
C CK+ + K ++ R++ CV CYE Y C C + I ++YK WH+ CF C C + LA F+++D+K C +C+ ++ KC P+ G + + F G WH CF C++C + + F+ E + C C
Sbjct: 8 CHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPV----GTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 152 HSP 4 Score: 74.3294 bits (181), Expect = 1.214e-15 Identity = 44/168 (26.19%), Postives = 72/168 (42.86%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAG 1251
++C CKE L+ Y YC R Y C +C + I + + H H F C C+ SL + ++E C +C+ ++ C +G ++ + F+ WHE CF C C + + F +++ +C CY+ +FA C C KV G
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVG--TRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 171 HSP 5 Score: 67.3958 bits (163), Expect = 2.987e-13 Identity = 31/92 (33.70%), Postives = 50/92 (54.35%), Query Frame = 1
Query: 1474 FTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGC--KFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
+ C C + L G K+ +++ PYC CY L+ C C +PI GC K +S++ RHWH CF C+ C +L + F ++ ++C +C
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPI----GCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTEC 93 HSP 6 Score: 55.4546 bits (132), Expect = 4.164e-9 Identity = 40/140 (28.57%), Postives = 58/140 (41.43%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIG 1062
C CK IG +LS + H WH CF C+ C+ L D + KL C + +CA N TR M+ H F C C + ++ +E++ +C+ CYE +FA C CK+ G
Sbjct: 41 CESCKQPIGCDSKDLSYKDRH-------WHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANP----NPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGG 169
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl3b (four and a half LIM domains 3b [Source:ZFIN;Acc:ZDB-GENE-030131-8356]) HSP 1 Score: 180.259 bits (456), Expect = 3.780e-53 Identity = 85/227 (37.44%), Postives = 123/227 (54.19%), Query Frame = 1
Query: 1078 LSFKDRHWHEKCFECFECHTSL-VDRPFATKDESLFCSECYDNK---FAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
LS + HWHE CF+C EC T + + + + + C C + C C + + G+ K Y+G+ WHE CF C C + IG KSF+P +I CV C E K A +C C + + GV YK +H+EC C C LAG+ ++ + YC C+ + ++KKCT C KPIT G KF++ + WH+ECF C +C N+ G+GF+ D+I C DC
Sbjct: 2 LSLDNLHWHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCA 228 HSP 2 Score: 119.013 bits (297), Expect = 7.428e-31 Identity = 65/231 (28.14%), Postives = 107/231 (46.32%), Query Frame = 1
Query: 901 AMDQEH-HPNHFACKNCDNSL-TGHRYILKEENPHCIKCYETK---FANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
++D H H F C C + Y+L+ + C C + ++C CK I +++K R WHE+CF C C ++ + F + ++C C + K A RC C K + Y+ +H EC +C+ C P+ + R + + C PC+E KY++KC+ C + I +T+ WH ECF C C +AG F ++ D +C DC ++
Sbjct: 3 SLDNLHWHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTE--KGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCANQI 231 HSP 3 Score: 90.8929 bits (224), Expect = 4.867e-21 Identity = 59/221 (26.70%), Postives = 92/221 (41.63%), Query Frame = 1
Query: 736 WHPACFNCSECKELLVDYI-YCWHLNKLY---CARHYGEKIRPRCATCDELIFNEEYTRAM-DQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVF--KAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
WH CF CSEC + Y ++ CA + C +C I A + H FAC C ++ ++ + +C+ C E K A C C + + K + +K+ +H +C C C+T L T+ E L+C C++ K++ +C C K AG + + +WH ECF C C + K F+ R I C C
Sbjct: 9 WHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPV--TEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDC 227 HSP 4 Score: 80.1073 bits (196), Expect = 2.924e-17 Identity = 52/207 (25.12%), Postives = 92/207 (44.44%), Query Frame = 1
Query: 619 AHVEAITEFQGVQCFECKTT----IGKSELSVRIDHNESGA------HSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKI--GCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDN 1203
A E + E + + C C T+ +G S +S + E A +WH CF C+ C E + + H +++YC +K RC TC + + E+ + +H C C+ L G + E +C C+E K++ C C++ I +K L+ WH +CF+C C ++ + F + + +FC +C +
Sbjct: 25 AATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPV-TEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCANQ 230
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: tes (testis derived transcript (3 LIM domains) [Source:ZFIN;Acc:ZDB-GENE-040718-59]) HSP 1 Score: 136.346 bits (342), Expect = 1.662e-35 Identity = 89/289 (30.80%), Postives = 133/289 (46.02%), Query Frame = 1
Query: 424 FLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQG----------------------------VQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDE----LIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHE--KCFECFECHTSLVDRPFATKDESLFCS 1188
++ LP P R R+L +Q+P D+ + L + E ++ E+F+K + L + V + G +C CK + + + +V + S H WHP CF CS C E+LVD +Y W KLYC RHY + RPRC CDE LIF++EYT+A +Q H HF C CD L G Y+++++ P C CY K+A +C+ C+ I +++ ++ + WH CF C C LV F +FCS
Sbjct: 89 YIELLPRDKQPIEGSQGAMYRRRQLARQLPEHDQDPSRCHDL-SAAEVKQMEEFVKKYKAEALGVGDVMLPHQVGGSAAFAQAAGEASAGERGVGDGAAQAQRDYRCNRCKQPMQQGDPAVFAERAGS-EHLWHPGCFCCSACDEILVDLVYFWKRGKLYCGRHYCDTERPRCGGCDEVWTQLIFSKEYTQAEEQNWHLKHFCCFECDCVLAGEIYVMEQDKPVCKPCYMKKYARSCQSCQGSIEPEAQRVTLGEFSWHADPTCFLCSCCGIPLVGLRFLPHQAMIFCS 375 HSP 2 Score: 78.5666 bits (192), Expect = 5.996e-16 Identity = 52/165 (31.52%), Postives = 74/165 (44.85%), Query Frame = 1
Query: 1099 WHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEY---RGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG--VTYKGTPWHKE--CFTCTNCTKQLAGIKFTSKDDKPYC-ADCYGELF 1569
WH CF C C LVD + K L+C Y + RC GC +V+ + EY + WH + F C EC + + ++ + +C PCY KYA+ C C I VT WH + CF C+ C L G++F +C ADC G++
Sbjct: 219 WHPGCFCCSACDEILVDLVYFWKRGKLYCGRHYCDTERPRCGGCDEVWTQLIFSKEYTQAEEQNWHLKHFCCFECDCVLAGEIYVMEQDKPVCKPCYMKKYARSCQSCQGSIEPEAQRVTLGEFSWHADPTCFLCSCCGIPLVGLRFLPHQAMIFCSADCRGKVV 383 HSP 3 Score: 61.2326 bits (147), Expect = 2.241e-10 Identity = 45/158 (28.48%), Postives = 69/158 (43.67%), Query Frame = 1
Query: 1282 WHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTP-----WHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSE--CFTCSKCTTNLVGRGFLTNEDNIMC 1734
WH CF C C + + + + ++ C Y + +C C EV + + + T WH + F C C LAG + + DKP C CY + +A+ C C I + ++ WH++ CF CS C LVG FL ++ I C
Sbjct: 219 WHPGCFCCSACDEILVDLVYFWKRGKLYCGRHYCDTERPRCGGCDEVWTQLIFSKEYTQAEEQNWHLKHFCCFECDCVLAGEIYVMEQDKPVCKPCYMKKYARSCQSCQGSIEPEA--QRVTLGEFSWHADPTCFLCSCCGIPLVGLRFLPHQAMIFC 374
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: PXN (paxillin [Source:HGNC Symbol;Acc:HGNC:9718]) HSP 1 Score: 122.479 bits (306), Expect = 4.378e-32 Identity = 70/229 (30.57%), Postives = 107/229 (46.72%), Query Frame = 1
Query: 889 EYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKY-EYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFA 1572
+ AM + HP HF C +C + + K+ P+C + Y + F+ C C E + + + ++ DR WH + F C +C + F +D +C + Y FA +C+GC+ +A M+ Y WH ECF+C EC P SF + Q C Y + CS C + I ++ +H E F C C KQL F ++DKPYC C+ +LF
Sbjct: 1 QVVTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCS---RAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCFIKLFG 226 HSP 2 Score: 117.857 bits (294), Expect = 2.167e-30 Identity = 69/217 (31.80%), Postives = 93/217 (42.86%), Query Frame = 1
Query: 1093 RHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
R WH + F C C + R F KD +C E Y + F+ RC C + R + WH E F C +C +P G + F RD + C Y +A KC+ C+ I + ++ T WH ECF C C F + +PYC Y E C+ C KPITG + IS + +H E F C+ C L F D C C
Sbjct: 8 RTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITG----RCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPC 220 HSP 3 Score: 94.7449 bits (234), Expect = 2.339e-22 Identity = 60/219 (27.40%), Postives = 93/219 (42.47%), Query Frame = 1
Query: 727 AHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCY 1380
+WHP F C C+ + + + YC Y PRCA C+E + N+ A+D+ HP HF C C + ++ P+C + Y FA C C I LS + WH +CF C EC T ++ F + +C Y + + C GC K R K++H E F+C C + + +F ++ + C PC+
Sbjct: 7 GRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAI--MQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITG--RCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCF 221 HSP 4 Score: 58.5362 bits (140), Expect = 5.146e-10 Identity = 30/99 (30.30%), Postives = 45/99 (45.45%), Query Frame = 1
Query: 1438 VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMC 1734
VT G WH E F C +C ++ F KD +PYC + Y LF+ +C C + + + ++ R WH E F C++C GF + C
Sbjct: 3 VTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVM--DNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYC 99
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: LIMPET (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX6]) HSP 1 Score: 360.147 bits (923), Expect = 9.989e-118 Identity = 192/531 (36.16%), Postives = 296/531 (55.74%), Query Frame = 1
Query: 163 QDI-GSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQ--KQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHS-WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
+DI G+ C++C + C+GF +H WRK C +C C +++H + +N E ++ + ++ +Q G W+P GL E++ ++S LP P + R ++L QIP+QD L + ++ + F + R L + V A Q C C + ++++ + G S WH CF C E ELLVD IYC +++YC RH+GEK++PRCA C+ELI+ EY++A+++ HP H C CD SL+ +++ E +P C +CY+ FAN CE C E IG SKD+ + +HWHE CF+C +C L++ F KDE L C C + C C F G +K Y+ K +H++CF+C ECKQPIG+K FI RD++ +C C+++K+A+ C KC +VI+ V + G+ +H ECFTC +C K LAG FT ++ + C +CY E +AK+C C I G KF++++ +++H ECFTC KC L G F + +C C
Sbjct: 27 EDIEGNKCLQCGNICRGFSVHPWRKFCNSCKCPHELHVLSTNNNDQNTPEERMGVEEEDPPMLQGSHLQQAMMEGYEWVPRGLTSEEIQIYMSLLPEDKIPKLKSLGAQHRTKQLSLQIPLQDFSSKHCKKLTLD-QKMAMDDFCEKRIKTALGVGAVRACMA-QPSTCKRCNGPVNPGDMALFAE--RLGPDSCWHVKCFTCEEDGELLVDLIYCSKDDEIYCCRHWGEKLKPRCAGCEELIYVGEYSQALEKNWHPGHLCCSYCDESLSNQKFVTVEGSPSCFRCYDENFANRCEACGEPIGPGSKDVDVRSKHWHEGCFKCSQCSKQLMNEGFTLKDEKLICHGCRGINPSKVCAACNGDFAPGEKKVGYQSKTFHDKCFICDECKQPIGSKQFIRRDERRLCNNCFDSKFAKVCVKCNQVIKTSSVQHAGSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIE--GNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPC 551 HSP 2 Score: 69.707 bits (169), Expect = 1.441e-12 Identity = 40/159 (25.16%), Postives = 71/159 (44.65%), Query Frame = 1
Query: 727 AHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDN 1203
+ ++H CF C ECK+ + + + C + K C C+++I A +H F C +CD L G + +E C CY ++A C C I ++K +++ ++++H +CF C +C+ L F +D C C D+
Sbjct: 397 SKTFHDKCFICDECKQPIGSKQFIRRDERRLCNNCFDSKFAKVCVKCNQVIKTSSVQHA-GSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIEGNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPCDDD 554
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41100 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D2]) HSP 1 Score: 359.377 bits (921), Expect = 1.184e-117 Identity = 196/542 (36.16%), Postives = 286/542 (52.77%), Query Frame = 1
Query: 151 MALKQDI--GSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDD-----NISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQ-CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKD-RHWHEKCFECFECHTSL-VDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
M L QDI G PC++C DRC GF LH W+K C C C + HD+ ++ +I+ ++ + L K I G W P GL +VE ++ LP P K ++ Q+PVQDR +K S E F++ +S D + F Q C C I +SE +V + N S+HP CF C C ELLV+ Y H +K+YC RH+ E + RC CDELIF EYT AM++ H HF C+ CD+S+TG ++I++ + P C C++ +A+ CE C +KIG +S+D+S D RHWH+KCF C C L + + + C++CY + C C ++ +G + EY G WHE CF C C + IG F+P+D C CY++K++++C+ C E + GGV Y G WHK CF+C C + LA F+ +D YC +CYG+ +AK+C C K I G ++ + E ++H ECF CS+C +L GF+ D ++C DC
Sbjct: 1 MILLQDIDEGKPCLKCKDRCPGFSLHVWKKACQYCKCPREDHDIQNEANETMDINTRINGIDLPPPP---KVI----SGGFPKYLWTPSGLSQSQVELYMKELPPQKVPIKDSPGSKYYRAQMAYQLPVQDRDPEKLSTFSA----AHRESFMQFNSSADNARGEGVVKVPFGRRQRCARCSHPILRSEPAV-VAENIGAEASFHPGCFTCETCNELLVELTYFQHADKVYCGRHFAELQKSRCGGCDELIFTGEYTVAMNKNWHLGHFQCQTCDHSITGRQFIVRGDKPVCTDCFKDSYAHECEACHQKIGPESRDISSDDDRHWHDKCFICDICRRPLKSEGSLHSTETKSCCNKCYVANYQKECCACGQIIDSGASRLEYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLLEGGVLYNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEICLKAIVGG---EYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCA 527 HSP 2 Score: 70.0922 bits (170), Expect = 1.074e-12 Identity = 43/161 (26.71%), Postives = 73/161 (45.34%), Query Frame = 1
Query: 718 ESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYD 1200
E + WH CF C+ C E + + + +C Y K RCA+C E + E + H F+C C SL + +++ +C++CY +A CE C + I + + ++ ++H++CF C C SL F + + L C +C D
Sbjct: 370 EYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLL-EGGVLYNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEICLKAI-VGGEYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCAD 528
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49801 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFY0]) HSP 1 Score: 209.534 bits (532), Expect = 5.175e-62 Identity = 115/363 (31.68%), Postives = 182/363 (50.14%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSF-----------YQKYIEAQKQGK---ASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGV-QCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWH--EKCFECFECHTSLVDRPFATKDESLFCS 1188
+ +++ G+ C++C D+C+G LH WRK C C C + HD+++D + +E ++ + F + K A Q K W+P G+ + ++ SLP P + K R ++ Q+P+ D + +L ++ ERQ+ E F RN L + + T+ CF C + E++V WHP CF C+ C LLVD IY + +YC RHY E+ +PRCA CDELIF+E YT+A D+ H HF C CD L G Y+ + PHC++CY+ +A C CK+ I D+K + + + WH +CF C +C+ ++ + F ++FCS
Sbjct: 21 LGHERNEGAKCLKCGDKCEGLDLHFWRKICKNCKCKQEDHDLYED----IANEHEVKSRLFKDSNVAHLRDYFNKLTAANDQPKTEFVKKFTWVPPGMNQGILVKYMESLPKELVPIVGSEGAKYRRAQMVYQLPIHDHDPNYCDNL-SDAERQKMEDFCAMRNQDALGVGDIREKTQAASKWNCFRCSKPVMTGEVAV-FASRAGEDKCWHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCS 377 HSP 2 Score: 96.2857 bits (238), Expect = 1.526e-21 Identity = 52/160 (32.50%), Postives = 82/160 (51.25%), Query Frame = 1
Query: 1282 WHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTY-KGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWH--SECFTCSKCTTNLVGRGFLTNEDNIMCP-DCGR 1749
WH CF+C C + + +D I C Y ++ +C+ C E+I T + WH+ F C C + L G + ++ +P+C +CY + +AK C C K I K I +G+ WH SECF C+KC +++G+ FL ++NI C DC +
Sbjct: 225 WHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIA--ADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCSVDCAK 382 HSP 3 Score: 90.5077 bits (223), Expect = 1.224e-19 Identity = 51/159 (32.08%), Postives = 79/159 (49.69%), Query Frame = 1
Query: 1087 KDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTK-VFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG--VTYKGTPWH--KECFTCTNCTKQLAGIKFTSKDDKPYCA 1548
+D+ WH CF C C+ LVD + KD ++C Y +F RC C + +F + E R WH+ F CLEC + +G + ++ R Q C+ CY+ YA+ C C + I + ++G WH ECF C C K + G +F + +C+
Sbjct: 221 EDKCWHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRN--WHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCS 377
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49798 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX7]) HSP 1 Score: 199.519 bits (506), Expect = 1.564e-58 Identity = 118/353 (33.43%), Postives = 182/353 (51.56%), Query Frame = 1
Query: 148 IMALKQDIGS-PCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKA-SGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEF-QGVQCFECKTTIGKSELSVRIDHNESGAHS-WHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWH--EKCFECFECHTSLVDRPFATKDESLFCS 1188
++A D GS C C D C GF H WRK C C C + H++ D+ + ++D G A W+P GL P +V S++++LP P+ ++ R R++ Q+P D +F + + E++E F+ R L V+ + E +G C EC + ++V + +G H+ WH +CF C+ CKELLVD IY + +K+YC RH+ E ++PRCA CDE+IF E+ T A D H HF C CD L G RY++++ P+C C+E+ +A C+ C E I D+ ++ +HWH +CF C C +L+ PF K ++CS
Sbjct: 1 MVAHDDDDGSKACNNCGDTCPGFSAHYWRKICRNCKCPREDHNIISDDAR-AGRNIYMADVHRASSLASDDDSGCALDEYAWVPPGLGPEQVHSYMNALPEEKVPYVDSIGERYRNRQIILQLPPHDNEA-RFCNGLSEEEKRELCFFVALRKRDSLGRGVVKQVPEMSEGYSCKECGECVTAGSMAVYA--SRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCS 349 HSP 2 Score: 95.9005 bits (237), Expect = 1.776e-21 Identity = 59/183 (32.24%), Postives = 89/183 (48.63%), Query Frame = 1
Query: 1219 CDGCTKVFKAG-MRKYEYRGKQ---WHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVT-YKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHS--ECFTCSKCTTNLVGRGFLTNEDNIMC-PDC 1743
C C + AG M Y R Q WH CF+C CK+ + + +D ++ C + +C+ C E+I T + + WH + F C C L G+++ +D+KPYC C+ L+A+ C C +PI ++ G+HWH+ ECF+C C L+G FL I C PDC
Sbjct: 172 CKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDA--SQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCSPDC 352 HSP 3 Score: 90.5077 bits (223), Expect = 1.092e-19 Identity = 58/186 (31.18%), Postives = 85/186 (45.70%), Query Frame = 1
Query: 1015 ETKFANTCEQCKEKIGCDSKDL--SFKDRH--WHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG--VTYKGTPWH--KECFTCTNCTKQLAGIKFTSKDDKPYCA 1548
E +C++C E + S + S +H WH CF C C LVD + KD ++C + RC C ++ A + E WH + F C EC P+G ++ RD + C C+E+ YA+ C C E I + + G WH ECF+C C K L G+ F K + YC+
Sbjct: 165 EMSEGYSCKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFA-EQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCS 349
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S548]) HSP 1 Score: 127.487 bits (319), Expect = 2.648e-33 Identity = 83/240 (34.58%), Postives = 110/240 (45.83%), Query Frame = 1
Query: 856 CATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKY-EYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFA 1572
CA C++ I + T A+ + HP HFAC C+ L + ++ P+C + Y FA C C I DS ++ D+ WH + F C EC D F +D FC E Y FA RC GC + M Y WH ECF+C EC+QP SF D + C Y K C C + I +T +H E F C C KQL F ++DKPYC C+ +LF
Sbjct: 26 CAACNKPIIGQVCT-ALGKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPI-LDSC-VTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPI---MDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITGRCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLFG 259 HSP 2 Score: 106.301 bits (264), Expect = 7.430e-26 Identity = 67/216 (31.02%), Postives = 90/216 (41.67%), Query Frame = 1
Query: 1099 WHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCT-KVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
WH + F C C L + F +D +C Y + FA RC C + + + + + WH E F+C EC P G F RD + C Y +A +C C + I ++ WH ECF CT C + G F D +PYC Y C C KPITG + I+ R +H E F C+ C L F D C C
Sbjct: 45 WHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALD---QTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITG----RCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPC 253 HSP 3 Score: 104.375 bits (259), Expect = 3.526e-25 Identity = 52/154 (33.77%), Postives = 74/154 (48.05%), Query Frame = 1
Query: 1273 GKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMC 1734
GK WH E F C+ C+ P+G ++F RD + C Y + +A +C+ C I VT WH E F C C F +D KP+C + Y +FA +C C +PI +IS HWH+ECF C++C G F ++ C
Sbjct: 42 GKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMD----NYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYC 191 HSP 4 Score: 95.9005 bits (237), Expect = 3.256e-22 Identity = 64/221 (28.96%), Postives = 91/221 (41.18%), Query Frame = 1
Query: 733 SWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYA 1395
+WHP F C C+ L + K YC R Y + PRCA C+ I + T A+DQ HP HF C C N + ++ P C + Y FA C C + I +S HWH +CF C EC F D +C Y K C C K R +++H E F+C C + + +F ++ + C PC+ +
Sbjct: 44 TWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVT-ALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPI--MDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITG--RCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLFG 259 HSP 5 Score: 58.151 bits (139), Expect = 1.859e-9 Identity = 34/111 (30.63%), Postives = 45/111 (40.54%), Query Frame = 1
Query: 1402 CSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMC 1734
C+ C + I T G WH E F C C L F +D KPYC Y + FA +C C PI ++ + WH E F C++C GF + C
Sbjct: 26 CAACNKPIIGQVCTALGKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILD----SCVTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFC 132
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:101169645]) HSP 1 Score: 354.755 bits (909), Expect = 5.382e-119 Identity = 156/273 (57.14%), Postives = 208/273 (76.19%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
+ C C SL G +Y+L+EENP+C+KCYE+ ++NTCE+CK+ IGC+++DLS+KDRHWHE CF+CF+C SLVD+PF+TKDE L C+ECY N+++++C C K G RK E++G WHE CF C C+QPIG KSFIP+D CVPCYE ++A +C C + I GGVTY+ PWHK+CF CT+C +QL+G +FTSKDD YC +C+ L+AKKC CT PI+G GG K+ISFE R WH++CF C KC+ +LVGRGFLT D+I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 146.362 bits (368), Expect = 2.323e-39 Identity = 86/273 (31.50%), Postives = 128/273 (46.89%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
++C CKE L Y YC + Y C C + I N D+ H + F C C SL + K+E C +CY ++++ C +CK+ I S+ + K WHE CF C C + + F KD +C CY+ +FA +C C K G YR + WH++CFLC CKQ + + F +D C+ C+ N YA+KC+ CT I G ++++ WH +CF C C+ L G F ++ D C +C
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 121.324 bits (303), Expect = 1.920e-30 Identity = 76/258 (29.46%), Postives = 119/258 (46.12%), Query Frame = 1
Query: 625 VEAITEFQGVQCFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
V+ C ECK IG +LS + H WH CF C +CK LVD + +L C Y + +C C + I +R M+ + H H F CK C + +I K+ + +C+ CYE +FA C CK+ I + ++++D+ WH+ CF C C L + F +KD+ +C C+ N +A +C CT G + + +QWH +CF C +C + + F+ I+C C
Sbjct: 29 VKCYESLYSNTCEECKKPIGCNTRDLSYKDRH-------WHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533]) HSP 1 Score: 336.65 bits (862), Expect = 3.081e-111 Identity = 149/283 (52.65%), Postives = 202/283 (71.38%), Query Frame = 1
Query: 895 TRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
++A+ + + F C NC SL G +YI ++P+CI CY++ F+NTC++CKE IG D+++L ++DRH+HE CF CF C SL D PF ++D +L C++CY N+F+++C C K+ G RK EY G WHE CF+C C+QPIG+KSFIP + CVPCYE+K+A +C++C + + +GGVTY+ PWHKECF CT+C QLAG FTS+D+ PYC C+ L+AKKC C KPITGFGG K+ISFE R WH CFTCS+C+ +LVG GF N D I+C DC
Sbjct: 40 SQALKKTTMADRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 322 HSP 2 Score: 150.599 bits (379), Expect = 1.998e-40 Identity = 85/277 (30.69%), Postives = 128/277 (46.21%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH-FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C CKE L Y + YC Y C C ELI ++ + H+ H F C CD SL + ++ C CY +F++ C C + + ++ L + WHE CF C C + + F + +C CY++KFA RC C K G YR + WH+ECF+C C+ + + F RD+ C+ C+ + YA+KC C + I G ++++ WH+ CFTC+ C+ L G F D+ C DC L
Sbjct: 52 FDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSNL 326 HSP 3 Score: 123.635 bits (309), Expect = 9.115e-31 Identity = 70/248 (28.23%), Postives = 116/248 (46.77%), Query Frame = 1
Query: 658 CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYEN 1386
C ECK IG + + +H CF C C L D + N L C Y + +C CD+++ TR ++ H F C +C+ + +I ++ +C+ CYE KFA C +CK+ + ++++D WH++CF C C T L + F ++DES +C +C+ + +A +C+ C K G + + +QWH+ CF C +C + F P +I+C C N
Sbjct: 87 CDECKELIGHDARELFYEDRH-----YHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 325
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533]) HSP 1 Score: 332.028 bits (850), Expect = 4.038e-110 Identity = 148/273 (54.21%), Postives = 197/273 (72.16%), Query Frame = 1
Query: 925 NHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
+ F C NC SL G +YI ++P+CI CY++ F+NTC++CKE IG D+++L ++DRH+HE CF CF C SL D PF ++D +L C++CY N+F+++C C K+ G RK EY G WHE CF+C C+QPIG+KSFIP + CVPCYE+K+A +C++C + + +GGVTY+ PWHKECF CT+C QLAG FTS+D+ PYC C+ L+AKKC C KPITGFGG K+ISFE R WH CFTCS+C+ +LVG GF N D I+C DC
Sbjct: 3 DRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275 HSP 2 Score: 149.443 bits (376), Expect = 1.910e-40 Identity = 85/277 (30.69%), Postives = 128/277 (46.21%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH-FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGEL 1566
F+C CKE L Y + YC Y C C ELI ++ + H+ H F C CD SL + ++ C CY +F++ C C + + ++ L + WHE CF C C + + F + +C CY++KFA RC C K G YR + WH+ECF+C C+ + + F RD+ C+ C+ + YA+KC C + I G ++++ WH+ CFTC+ C+ L G F D+ C DC L
Sbjct: 5 FDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSNL 279 HSP 3 Score: 122.094 bits (305), Expect = 9.690e-31 Identity = 70/248 (28.23%), Postives = 116/248 (46.77%), Query Frame = 1
Query: 658 CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMD---QEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYEN 1386
C ECK IG + + +H CF C C L D + N L C Y + +C CD+++ TR ++ H F C +C+ + +I ++ +C+ CYE KFA C +CK+ + ++++D WH++CF C C T L + F ++DES +C +C+ + +A +C+ C K G + + +QWH+ CF C +C + F P +I+C C N
Sbjct: 40 CDECKELIGHDARELFYEDRH-----YHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 278
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl5 (four and a half LIM domains protein 2-like [Source:NCBI gene;Acc:101156370]) HSP 1 Score: 332.028 bits (850), Expect = 4.418e-110 Identity = 147/274 (53.65%), Postives = 195/274 (71.17%), Query Frame = 1
Query: 928 HFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
F C C +SL G +YI+KE+ +C KCYE FAN CE+C IGC+SKDLS+KDRHWHE+CF+C +C SL D+ FA +++ L C+ECY ++++C C K G RK EY+G WHE CFLC C+QP+G KSFIP++ CVPC+E ++A +C C + I GGVTY+ PWH+ECF C C +QL+G +FTS+++ PYC +C+ L+AKKC CTKPIT G K+ISFE R WHSECF C +C+ +LVGRGFLT D+I+C +CGR
Sbjct: 6 RFDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGR 279 HSP 2 Score: 147.517 bits (371), Expect = 8.000e-40 Identity = 85/273 (31.14%), Postives = 127/273 (46.52%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIF-NEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C CK+ L+ Y + YC + Y C C I N + D+ H F C C SL + +E+ C +CY +++ C CK+ I S+ + +K WHE CF C C L + F K+ FC C++ +FA +C C K G Y+ K WH ECFLC+ CK+ + + F R+ C+ C+ N YA+KC CT+ I ++++ WH ECF C C+ L G F ++ D C +C
Sbjct: 7 FDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTG--GVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTEC 277 HSP 3 Score: 127.102 bits (318), Expect = 1.823e-32 Identity = 77/251 (30.68%), Postives = 119/251 (47.41%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNH---FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENK 1389
C EC T IG +LS + H WH CF C++C L D + + L C Y +C TC + I +R M+ + + H F C C L +I KE C+ C+E +FA C CK+ I + ++++D+ WH +CF C C L + F +++ +C EC+ N +A +C GCTK AG + + +QWH ECF C++C + + F+ + I+C C K
Sbjct: 42 CEECSTPIGCNSKDLSYKDRH-------WHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPG--SRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAI--TTGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGREK 281
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: rragcb (Ras-related GTP binding Cb [Source:ZFIN;Acc:ZDB-GENE-070705-380]) HSP 1 Score: 305.064 bits (780), Expect = 3.373e-94 Identity = 132/271 (48.71%), Postives = 181/271 (66.79%), Query Frame = 1
Query: 931 FACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
F C+NC SL G +YI E PHCI CY+ ANTC++C E IG + K+L+FKDR++HE CF CF C SL + F + ++ L C++CY N+F+++C C K+ G + E+ G WHE+CF+C C++PIG +SF+P + Q CVPCYE ++ +CS C + + +GGV+Y+G WH+ECF CT C+ LAG FTS+ + PYC C+ L+AKKC C ITGFG K+ISFE R WH CF CS+C+ +LVG GF D I+C DC
Sbjct: 449 FDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC 719 HSP 2 Score: 150.984 bits (380), Expect = 3.946e-38 Identity = 86/273 (31.50%), Postives = 125/273 (45.79%), Query Frame = 1
Query: 751 FNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI-FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
F+C CK L Y N+ +C Y + C C E+I N++ D+ +H + F C CD SL+ + E+ C CY +F++ C C + + SK L F WHE CF C C + F + +C CY+ +F RC C K G Y+G WH ECFLC C P+ + F + + CV C+ N YA+KC+ C I G ++++ WH+ CF C+ C+ L G F D+ C DC
Sbjct: 449 FDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKG--GVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC 719 HSP 3 Score: 113.235 bits (282), Expect = 8.375e-26 Identity = 64/246 (26.02%), Postives = 110/246 (44.72%), Query Frame = 1
Query: 658 CFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKA-GMRKY-EYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYEN 1386
C EC IG ++ + +H CF C C L + + H + L C Y + +C +C++++ D H + F C +C+ + G ++ E +C+ CYE +F C C + + +S++ WH +CF C C L +PF ++ E+ +C +C+ N +A +C GC G KY + ++WH+ CF C C + F P QI+C C ++
Sbjct: 484 CQECTEIIGHNDKELTFKD-----RYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKAL--TKGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDCNDD 722 HSP 4 Score: 101.293 bits (251), Expect = 6.447e-22 Identity = 52/153 (33.99%), Postives = 77/153 (50.33%), Query Frame = 1
Query: 1291 ECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG--VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 1743
E F C CK + + +I + + C+ CY+ +A C +CTE+I +T+K +H+ CF C C + L+ FTS +D C DCY F+ KC C K + G K + F+G WH +CF C C + G F+ NE+ C C
Sbjct: 447 ERFDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIV--MPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPC 597
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1) HSP 1 Score: 1087.79 bits (2812), Expect = 0.000e+0 Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSRLRRLRKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR
Sbjct: 1 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSRLRRLRKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 533
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000072127.1 (SMESG000072127.1) HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0 Identity = 363/533 (68.11%), Postives = 444/533 (83.30%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MALKQDIG+PC++C D+C+GF++HGWRKTC C+CS D HD+ D++ +L+EMKL+DK +Y ++E+ K + GL+WLPIGL+ EVE F S + + +K R ++LR+QIPV DR ++KFSHL+T ERQEAEKFL+ RN+ DL + HV + + + C +C+++I +L+VR++H + SWHP CF C+EC E+LVDYIYCW+ NK YC RHY + IRPRCATCDELIF EEYTRAM+QEHHP HFACK+CDNSLTGHRYILK++ P CI CYE+++AN C+QC +IGCDSKDLSFKDRHWHEKCF+CFEC +SLVD+PFATKD +L+CS+CYDNKFA RCDGC KVFKAGMRKYEYRGKQWHEECFLC++C+QPIG KSFIPR+Q I+CVPCYE KYAQKC+KC VIRRGGVTYKG+PWHKECFTCT+C+KQLAGIKFTSK++KPYCA+CYGELFAKKC+KCTKPITGFGGCKFISFE RHWHSECFTC KC +LVG+GFLTN+D I+CPDCGR
Sbjct: 1 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRL-DMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 529
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1) HSP 1 Score: 583.948 bits (1504), Expect = 0.000e+0 Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 1
Query: 904 MDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 1749
MDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR
Sbjct: 1 MDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 282 HSP 2 Score: 167.933 bits (424), Expect = 2.571e-47 Identity = 91/277 (32.85%), Postives = 135/277 (48.74%), Query Frame = 1
Query: 739 HPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEH-HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGG----VTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADC 1554
HP F C C L + Y +C + Y K C C E I + + H H F C C SL + K+E+ C +CY+ KFA C+ C + + ++ + WHE+CF C EC + + F +D+ + C CY+NK+A +C CT+V + G Y+G WH+ECF C C + + F +D + C CY +A+KC+KCT+ I G ++++G WH ECFTC+ CT L G F + +D C DC
Sbjct: 6 HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRG--GVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 280 HSP 3 Score: 120.939 bits (302), Expect = 2.219e-30 Identity = 73/245 (29.80%), Postives = 111/245 (45.31%), Query Frame = 1
Query: 658 CFECKTTIG--KSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELI-FNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFK--AGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPC 1377
C +CK IG +LS + H WH CF C EC LVD + L+C+ Y K RC C ++ ++ H F C C + +I +++ C+ CYE K+A C +C E I +++K WH++CF C C L F +KD+ +C++CY FA +C CTK G + + G+ WH ECF C +C + + F+ + I+C C
Sbjct: 45 CEQCKEKIGCDSKDLSFKDRH-------WHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVI--RRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 280
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000009923.1 (SMESG000009923.1) HSP 1 Score: 483.026 bits (1242), Expect = 3.388e-165 Identity = 255/537 (47.49%), Postives = 338/537 (62.94%), Query Frame = 1
Query: 202 CKGFQLHGWRKTCTACNCSYDIH----DVFDDNIS----CV---LDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFL---SSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKF-SHLDTNVER----QEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSEL-SVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNK--LYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
C + H WR C +C C H + D S CV +D + + + Q Y A +++ L WLPIGL+ E+ FL + + + + R + QIP DR + +H D E Q A F K+R+++ L +E + Q V C C ++ +RI H S + H +CF C CKE LVD+ YCW + ++C RH+GE RCA CDELI N+++T+AMDQE H HFAC CD+SLTG +YIL+++NP C+KC+E +F NTC +C EKI CDSKDLSFK+ HWHEKCF C CH SLVDR F TKD L+C C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C +C Q IG KSFIP + +I C+ CYENK+AQKC KC+E+IR+ G+TYKG PWHKECF CT+C K L+ KFTSK++KPYC +C+ ELF K+C KCT+PI+GFGGCKFIS HWH+ECF CSKC ++VG+GF ED ++C CG
Sbjct: 9 CDKYTNHKWRFRCVSCRCPNYRHVSHVNSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDN-QEV-CSICDKQFEDEDIFCIRITH--SAEFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDICHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 541
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000004969.1 (SMESG000004969.1) HSP 1 Score: 456.062 bits (1172), Expect = 4.605e-155 Identity = 236/533 (44.28%), Postives = 329/533 (61.73%), Query Frame = 1
Query: 151 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIE-VESFLSSLPASANPHGVGDDDKSXXXXXXKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCG 1746
MALKQDIG C C++ C GF++HGWRKTC C C+ D HD+FD++ +LDEMK+ DK +Y EA G WLP+GLK +E F+ + A P+ + R + LR QIPVQDR ++KF+HL T ER+EAEKFL NRN+ D+ + V E + C EC T I + L V + + WH CF C+ C+ELLVDYIY + KLYC RHY + IRPRC+ CDELIF+ EY RA D E H NHFAC CD SL+ Y+ + CI+CY + +N C +C ++I CDSKDLS+ + HWH CF C C+ +L F +E L+C C+ +K +C C + + G + + K WH CF C +CK+PIG SF+ +D++ IC C++ KYA++C+KC ++++ GG++YK +H +CF C NC ++A KF K+++ YC C LF+ +C KCTKPITG CK+ISFE ++WH CF C KC +LV +GF +++ +CP+C
Sbjct: 1 MALKQDIGLSCNNCSN-CTGFKIHGWRKTCDKCKCAVDKHDIFDESTLPILDEMKV-DKPISAEYKEALAVAGKVGKPWLPVGLKNKNMIEEFILN---KAYPNLL-----KRKKCLRLQIPVQDRCVEKFNHLGTLAEREEAEKFLLNRNNRDIGLGVVRISDEH--MNCTECHTNIKPNTLCVTNEQTSAVKQFWHLECFKCTSCQELLVDYIYASYNQKLYCIRHYAKNIRPRCSECDELIFSVEYIRAGDNEFHLNHFACYICDASLSKQSYLQHKGFATCIQCYNHELSNKCVKCSKQISCDSKDLSYNNNHWHSDCFMCSICNKNLEGNQFLYLNELLYCVNCHQDKILPKCISCDRPIENGAKMICFDEKNWHFNCFNCKDCKRPIGNDSFVQQDKRSICSKCFDAKYAKRCAKCEKLVKEGGISYKNQSYHYDCFNCANCKCRIADTKFLVKNNENYCKVCCDRLFSTRCKKCTKPITGDAICKYISFEDKNWHVGCFNCDKCHVSLVDKGFFNKDNDTLCPNCS 521 The following BLAST results are available for this feature:
BLAST of Four and A half lim domains vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Four and A half lim domains vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014320 ID=SMED30014320|Name=Four and A half lim domains|organism=Schmidtea mediterranea sexual|type=transcript|length=1793bpback to top protein sequence of SMED30014320-orf-1 >SMED30014320-orf-1 ID=SMED30014320-orf-1|Name=SMED30014320-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=534bp MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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