SMED30014307

Overview
NameSMED30014307
Smed IDSMED30014307
Length (bp)1992
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014307 (SMED30014307) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014307 (SMED30014307) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014307 (SMED30014307) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014307 (SMED30014307) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30014307

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neuronSMED30014307SMESG000064621.1 dd_Smed_v4_5431_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30014307SMESG000064621.1 dd_Smed_v4_5431_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
parenchymal cellSMED30014307SMESG000064621.1 dd_Smed_v4_5431_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014307 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001194:92787..94543 -6932
Homology
BLAST of SMED30014307 vs. TrEMBL
Match: A0A183VS77 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS3333 PE=4 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 7.176e-23
Identity = 108/450 (24.00%), Postives = 194/450 (43.11%), Query Frame = 3
Query:  138 MYQNSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVPNDDAEKVLLKFDDEIEPETHDHYPKTLLQS----LDNGLC-----YQVELVGSWQNFLGR--------------KAGKVQCSSVFRSTMFVVFENESQIFRMTLSENGISN-----DSIWFPKFQ-----DSSIYPIEI-----KCSNEVIYVSGKINTSEDVT---QYLIVKYNMDENVLAYTRRYYYPKFDKFDVD-HDGNLIILSPQIDDEGLN-------------SQISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSL------KKQSDDQKCPEYRLMCFRCCINKTNGD-KSQLRAALESSRWRMAGFSLGPLVVLD-DQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNL-----------IGMCSDGRDYAYLFTEHNVFRFRFQE 1265
            M  +  +    + + PVK+R    + + R + IVS+Y+P ++  +  L  +DE    + DH    LL S    +D  L      Y ++L+ S +  +                ++ K+ C +    T+F+  E    +F ++  +N  ++     +SI+            +I+P+E+     K  + V+YV G +  SE VT    +++ K++    +LA +R Y Y ++    VD +DG L++  P I++ G                QI KL+  F  R + IT+G +   Y P YV      +  W S+      K+ +++Q  P  RL+ F     + NG+ K   R  L +  W  A    G +   D D++L ID E   L YI+W  ++  P + +I+ P+S  +           +G+       AY     ++F F F+ 
Sbjct:   16 MTGDENLDVVEQGIHPVKRRPFSKSLRSRLNHIVSMYIPGNNPVENRLDGNDE----SADH--AGLLSSNELKMDFSLTKDNDIYDIKLLYSSEKIIQSIMDNVKLSDKYSIGESMKITCLAADHKTIFIGLERLPYVFLISSEKNDTTSQKSGGNSIFKGYLHLHTPGIGNIFPVELCSHSTKPDHTVLYVVGTVQKSESVTVKQNWILAKFDTSGQLLARSRVYPYQRYSGLSVDTNDGQLLLACPSINESGSPYNNTTTNKCNDSPGQICKLTPGFERRIFSITMGKESVFYKPNYVTQETAGHCCWASVERFAQGKENTENQHQPTRRLLAFPG--RQPNGEVKQSARDWLHTYSWDFADLKPGRITAFDADRLLVIDSETGTLSYITWPSDQEKPSLYRITRPSSKPIRLLYSVYNNSQVGLSDAQTKVAYFVAGSSIFSFSFRH 457          
BLAST of SMED30014307 vs. TrEMBL
Match: A0A0R3WN58 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS2183 PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.180e-21
Identity = 110/428 (25.70%), Postives = 183/428 (42.76%), Query Frame = 3
Query:  132 QPMYQNSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVPNDDAEKVLL-----------KFDDEIEPETHDHYPKTLLQSLDN-------GLCYQV------ELVGSWQNFLGRKAGKVQCSSVFRSTM-----FVVFENESQIFRMTLSENGISNDSIWFP---------KFQDSSIYPIEI---KCSNEV--IYVSGKINTSEDVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVDHDGNLII--LSPQIDDEG---LNSQISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQSDDQKCPEYRLMCFRCCINKTNGDKSQLRAA---LESSRWRMAGFSLGPLVVLDDQ-ILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCSDGR--DYAYLFTEHNVFRF 1253
            +P+Y+  EI      V P ++R    + KDR + IVS+YVPN  + +  L             DD      +DH+    L  L+N       GLC+ V       LV S  N L   A  +  S    S M     ++ F  +S  F     E  + N S+  P           +   + P ++    C NE    YV G       +  +++  Y++D  ++A TR+Y Y ++   DVD DGN+++   S  IDD+     N+QI KL+  F  R + +TL    + Y P ++       + W S+ +   + K    R++ F     +   +   +R+    L  + W    FS G +  LD Q +L ID E  +L  I+W+E +    +++++ P +  +   C        AYL +E ++F F
Sbjct:    8 EPVYR--EITEDAPMVVP-QRRPMVRSLKDRITNIVSMYVPNGSSHESPLFASKNSQLKHDLVDDNDVAHINDHFGLLSLGELENQVESGKSGLCHIVPFIDKGTLVTSILNELQNSAYTISPSDFVISCMATDSIYLYFGVQSLPFLFKF-EIALLNTSLERPYPVTKIHLKTAESVDLIPSKVCLKSCRNETSEFYVIGTAQLEASLI-HILACYSIDGRLIAQTRKYLYQRYLAIDVDTDGNILLGCTSRSIDDDANHPSNAQICKLTPHFERRIFSVTLRKGSTSYCPDWITRSCVQGQCWASVSRWDSETKRMVRRIVAFPGVQPECESEGEVVRSPKEWLHVTSWSFEVFSAGSVFALDSQRLLTIDAEQRSLAMITWTEGQKGVSLQRLTKPGARKIDHFCVSNTYPPVAYLASEGDIFTF 430          
BLAST of SMED30014307 vs. TrEMBL
Match: C1LES2 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.385e-19
Identity = 104/437 (23.80%), Postives = 196/437 (44.85%), Query Frame = 3
Query:  147 NSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVP-NDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGL---------CYQVELVGSWQNFLG---RKAG-----------KVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQD---------SSIYPIEI-----KCSNEVIYVSGKINTSE--DVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVD-HDGNLIILSPQIDDEGLNS------QISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQ---SDD----QKCPEYRLMCFRCCINKTNGD-KSQLRAALESSRWRMAGFSLGPLVVLD-DQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCS----------DGRDYAYLFTEHNVFRFRF 1259
            NS+I     ++ PV++R    + ++R ++IVS+Y+P N   E  L+  DD     T DH    LL S D+ L          Y ++LV      +    R AG           K+   +   + ++   EN   +F ++   +   N +     F+           +I P E+     K ++  +YV G I T    D  ++++ K++     +A +R Y Y ++    VD +D +L++  P I++   +S      QI KL+  F  R + IT+G++   Y P YV      +  W S++++   ++D    ++ P  RL+ F     + +G+ K  +R  L +  W  A      +   D D++L +D +   L YI+W+++E  P++ +I+ P+S ++  +CS            ++ AY     +++ F F
Sbjct:   23 NSQIIVEQGTIHPVRRRPFSKSLRNRLNQIVSMYIPGNIPIESRLVGIDD-----TTDH--TGLLSSNDSRLGFASSKDNELYDIKLVYPGDQLIKGVLRHAGVSDKLSRDELPKITSLAADHNAIYFGLENLPYVFLISAKSHDWKNQTFESDHFKGCLHLYSPGVGNILPHELCTHSTKSNHTFLYVVGTIQTDNINDEKKWILAKFDSSGQQIARSRIYSYQRYSGLSVDTNDNHLLLACPSINESVASSNVNSPGQICKLTPGFERRVFSITMGNESVFYKPNYVTQETAGHCCWASVERRHYSTEDAENIRRPPTRRLLAFPG--RQQSGELKQSVREWLHAYSWDFADLKPCRIAAFDADRLLVVDADNGTLSYITWAKHEEKPVLYRITRPSSKHIRLLCSTYNSKVMLSDSPKNIAYFVAGSSIYSFSF 450          
BLAST of SMED30014307 vs. TrEMBL
Match: A0A4Z2DCZ5 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_002202 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.436e-19
Identity = 104/437 (23.80%), Postives = 196/437 (44.85%), Query Frame = 3
Query:  147 NSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVP-NDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGL---------CYQVELVGSWQNFLG---RKAG-----------KVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQD---------SSIYPIEI-----KCSNEVIYVSGKINTSE--DVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVD-HDGNLIILSPQIDDEGLNS------QISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQ---SDD----QKCPEYRLMCFRCCINKTNGD-KSQLRAALESSRWRMAGFSLGPLVVLD-DQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCS----------DGRDYAYLFTEHNVFRFRF 1259
            NS+I     ++ PV++R    + ++R ++IVS+Y+P N   E  L+  DD     T DH    LL S D+ L          Y ++LV      +    R AG           K+   +   + ++   EN   +F ++   +   N +     F+           +I P E+     K ++  +YV G I T    D  ++++ K++     +A +R Y Y ++    VD +D +L++  P I++   +S      QI KL+  F  R + IT+G++   Y P YV      +  W S++++   ++D    ++ P  RL+ F     + +G+ K  +R  L +  W  A      +   D D++L +D +   L YI+W+++E  P++ +I+ P+S ++  +CS            ++ AY     +++ F F
Sbjct:   23 NSQIIVEQGTIHPVRRRPFSKSLRNRLNQIVSMYIPGNIPIESRLVGIDD-----TTDH--TGLLSSNDSRLGFASSKDNELYDIKLVYPGDQLIKGVLRHAGVSDKLSRDELPKITSLAADHNAIYFGLENLPYVFLISAKSHDWKNQTFESDHFKGCLHLYSPGVGNILPHELCTHSTKSNHTFLYVVGTIQTDNINDEKKWILAKFDSSGQQIARSRIYSYQRYSGLSVDTNDNHLLLACPSINESVASSNVNSPGQICKLTPGFERRVFSITMGNESVFYKPNYVTQETAGHCCWASVERRHYSTEDAENIRRPPTRRLLAFPG--RQQSGELKQSVREWLHAYSWDFADLKPCRIAAFDADRLLVVDADNGTLSYITWAKHEEKPVLYRITRPSSKHIRLLCSTYNSKVMLSDSPKNIAYFVAGSSIYSFSF 450          
BLAST of SMED30014307 vs. TrEMBL
Match: A0A4Z2DJ09 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_000432 PE=4 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.175e-19
Identity = 105/437 (24.03%), Postives = 197/437 (45.08%), Query Frame = 3
Query:  147 NSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVP-NDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGL---------CYQVELVGSWQNFLG---RKAG-----------KVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQD---------SSIYPIEI-----KCSNEVIYVSGKINTSE--DVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVD-HDGNLIILSPQIDD----EGLNS--QISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQ---SDD----QKCPEYRLMCFRCCINKTNGD-KSQLRAALESSRWRMAGFSLGPLVVLD-DQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCS----------DGRDYAYLFTEHNVFRFRF 1259
            NS+I     ++ PV++R    + ++R ++IVS+Y+P N   E  L+  DD     T DH    LL S D+ L          Y ++LV      +    R AG           K+   +   + ++   EN   +F ++   +   N +     F+           +I P E+     K ++  +YV G I T    D  ++++ K++     +A +R Y Y ++    VD +D +L++  P I++      +NS  QI KL+  F  R + IT+G++   Y P YV      +  W S++++   ++D    ++ P  RL+ F     + +G+ K  +R  L +  W  A      +   D D++L +D +   L YI+W+++E  P++ +I+ P+S ++  +CS            ++ AY     +++ F F
Sbjct:   23 NSQIIVEQGTIHPVRRRPFSKSLRNRLNQIVSMYIPGNIPIESRLVGIDD-----TTDH--TGLLSSNDSRLGFASSKDNELYDIKLVYPGDQLIKGVLRHAGVSDKLSRDELPKITSLAADHNAIYFGLENLPYVFLISAKSHDWKNQTFESDHFKGCLHLYSPGVGNILPHELCTHSTKSNHTFLYVVGTIQTDNINDEKKWILAKFDSSGQQIARSRIYSYQRYSGLSVDTNDNHLLLACPSINELVATSNVNSPGQICKLTPGFERRVFSITMGNESVFYKPNYVTQETAGHCCWASVERRHYSTEDAENIRRPPTRRLLAFPG--RQQSGELKQSVREWLHAYSWDFADLKPCRIAAFDADRLLVVDADNGTLSYITWAKHEEKPVLYRITRPSSKHIRLLCSTYNSKVMLSDSPKNIAYFVAGSSIYSFSF 450          
BLAST of SMED30014307 vs. Planmine SMEST
Match: SMESG000064621.1 (SMESG000064621.1)

HSP 1 Score: 823.543 bits (2126), Expect = 0.000e+0
Identity = 395/395 (100.00%), Postives = 395/395 (100.00%), Query Frame = 3
Query:  111 MNQEINYQPMYQNSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVPNDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGLCYQVELVGSWQNFLGRKAGKVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQDSSIYPIEIKCSNEVIYVSGKINTSEDVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVDHDGNLIILSPQIDDEGLNSQISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQSDDQKCPEYRLMCFRCCINKTNGDKSQLRAALESSRWRMAGFSLGPLVVLDDQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCSDGRDYAYLFTEHNVFRFRFQEQLIKDSELLV 1295
            MNQEINYQPMYQNSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVPNDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGLCYQVELVGSWQNFLGRKAGKVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQDSSIYPIEIKCSNEVIYVSGKINTSEDVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVDHDGNLIILSPQIDDEGLNSQISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQSDDQKCPEYRLMCFRCCINKTNGDKSQLRAALESSRWRMAGFSLGPLVVLDDQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCSDGRDYAYLFTEHNVFRFRFQEQLIKDSELLV
Sbjct:    1 MNQEINYQPMYQNSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVPNDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGLCYQVELVGSWQNFLGRKAGKVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQDSSIYPIEIKCSNEVIYVSGKINTSEDVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVDHDGNLIILSPQIDDEGLNSQISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQSDDQKCPEYRLMCFRCCINKTNGDKSQLRAALESSRWRMAGFSLGPLVVLDDQILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCSDGRDYAYLFTEHNVFRFRFQEQLIKDSELLV 395          
BLAST of SMED30014307 vs. Planmine SMEST
Match: SMESG000073901.1 (SMESG000073901.1)

HSP 1 Score: 357.836 bits (917), Expect = 8.613e-118
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 1
Query: 1378 KPASLKTRRGYFYSSQRIRRPELFMNFGKDVNENCKTICKKRXXXXXXXXXXTEQSSKDTSFQIFSLDKILIEKSIHTERIYGINPLKTIHDQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYKVTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMVVPGLEKVTLAGVLS 1941
            KPASLKTRRGYFYSSQRIRRPELFMNFGKDVNENCKTICKKRNLSSSNSNNLTEQSSKDTSFQIFSLDKILIEKSIHTERIYGINPLKTIHDQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYKVTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMVVPGLEKVTLAGVLS
Sbjct:  207 KPASLKTRRGYFYSSQRIRRPELFMNFGKDVNENCKTICKKRNLSSSNSNNLTEQSSKDTSFQIFSLDKILIEKSIHTERIYGINPLKTIHDQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYKVTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMVVPGLEKVTLAGVLS 394          
BLAST of SMED30014307 vs. Planmine SMEST
Match: SMESG000073901.1 (SMESG000073901.1)

HSP 1 Score: 313.153 bits (801), Expect = 9.121e-101
Identity = 172/188 (91.49%), Postives = 172/188 (91.49%), Query Frame = 1
Query: 1378 KPASLKTRRGYFYSSQRIRRPELFMNFGKDVNENCKTICKKRXXXXXXXXXXTEQSSKDTSFQIFSLDKILIEKSIHTERIYGINPLKTIHDQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYKVTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMVVPGLEKVTLAGVLS 1941
            KPASLKTRRGYFYSSQRIRRPELFMNFGKDVNENCKTICKKRNLSSSNSNNLTEQSSKDTSFQIFSLDKILIEKS                DQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYKVTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMVVPGLEKVTLAGVLS
Sbjct:  207 KPASLKTRRGYFYSSQRIRRPELFMNFGKDVNENCKTICKKRNLSSSNSNNLTEQSSKDTSFQIFSLDKILIEKS----------------DQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYKVTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMVVPGLEKVTLAGVLS 378          
BLAST of SMED30014307 vs. Planmine SMEST
Match: SMESG000062352.1 (SMESG000062352.1)

HSP 1 Score: 135.961 bits (341), Expect = 1.909e-34
Identity = 95/343 (27.70%), Postives = 164/343 (47.81%), Query Frame = 3
Query:  252 PNDDAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGLCYQVELVGSWQNFLGRKAGKVQCSSVFRSTMFVVFENESQIFRMTLSE--NGISNDSIWFPKFQDSSIYPIEIKCSNEV-IYVSG----KINTSEDVTQYLIVKYNMDENVLAYTRRYYYPKFDKFDVDHDGNLIILSPQIDDEGLNSQISKLSYEFNSRQYYITLGDQQSKYTPLYVAAHGTSNRTWVSLKKQSDDQKCPEYRLMCFRCCINKTNGDK-SQLRAALESSRWRMAGFSLGPLVVLDDQ-ILAIDEELHNLCYISWSENEGHPIVKKISSPNSPNLIGMCSDGRDYAYLFTEHNVFRF 1253
            PN D E   L  +D+ +P       K +   ++  + Y V+ V    +       ++QC SV  + +F+   N   IF ++  E    +  +  W P  +D  +YP++IK   +  IY+S     +IN  E    +L++KY+    VL  T++Y +P+F  FD D DG +I++S   +D  +   +SKL+ +F+   Y        SKYTP  +   G + ++W+   K  +D+K P  RL+ F  C  +   +  +     + +S W    F+   +    ++   +IDE+   L  +SW EN   P V +IS+   P    +CS+    AYLFT H+++ F
Sbjct:   60 PNLDYELGQLDINDKSQP-------KMISDQINGDIFYTVQRVSKLSDIFLNPV-EIQCCSVIGNKLFIGIVNCIDIFILSFEEVDKKLHVERTWNPILRDLPLYPLQIKADRDGNIYLSAYYYPRINQDEKC--HLLIKYDCQFKVLIKTKKYCFPRFSDFDFDSDGCIIVISSSKEDNTV-YHVSKLTEDFSRFCYKFNFSSSTSKYTPHRIICLGIAEKSWIVAYKDENDEKEP--RLISFNSCACRDKDESMNSFMTKINTSAWSSGSFNPREICKFTNKYTFSIDEKTKALYCLSWDENNNCPDVAQISNIGEPCFQNICSNSNGTAYLFTHHSIYEF 389          
The following BLAST results are available for this feature:
BLAST of SMED30014307 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183VS777.176e-2324.00Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A0R3WN581.180e-2125.70Uncharacterized protein OS=Hydatigena taeniaeformi... [more]
C1LES21.385e-1923.80Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A4Z2DCZ51.436e-1923.80Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A4Z2DJ092.175e-1924.03Uncharacterized protein OS=Schistosoma japonicum O... [more]
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BLAST of SMED30014307 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014307 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000064621.10.000e+0100.00SMESG000064621.1[more]
SMESG000073901.18.613e-118100.00SMESG000073901.1[more]
SMESG000073901.19.121e-10191.49SMESG000073901.1[more]
SMESG000062352.11.909e-3427.70SMESG000062352.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014307 ID=SMED30014307|Name=SMED30014307|organism=Schmidtea mediterranea sexual|type=transcript|length=1992bp
TTCCGATCTATGAAATATATGCGACCAATCAAATCAAAGCCTAAAAATAG
CAACCAATTGTGTCAATGACAAAGTAGAGTTTATTGATTATATCTGATGA
GAAATTTAAAATGAATCAAGAAATTAATTATCAACCCATGTATCAAAATT
CTGAAATTCAGTCAGCAAATAAATCGGTAACTCCAGTTAAGAAACGAAAC
GAAAAAGGTAATTTTAAAGATAGATTTTCAAAAATCGTTAGTGTTTATGT
TCCAAACGATGATGCTGAAAAGGTGTTATTGAAATTTGACGATGAAATTG
AACCTGAAACCCACGATCATTATCCGAAAACTTTGCTACAGAGTCTTGAT
AACGGATTGTGTTATCAAGTGGAATTAGTTGGAAGTTGGCAAAATTTTCT
TGGCAGAAAAGCAGGAAAAGTTCAATGTAGTTCTGTTTTCAGGTCAACCA
TGTTTGTTGTGTTTGAAAACGAAAGCCAAATTTTTAGAATGACATTATCA
GAAAATGGGATCAGTAATGATAGTATATGGTTTCCTAAATTTCAAGATAG
TTCGATTTATCCTATAGAAATCAAATGCTCTAATGAAGTAATTTATGTGA
GCGGGAAAATAAACACTTCGGAAGACGTAACTCAGTATCTTATTGTAAAA
TACAATATGGATGAGAATGTATTGGCATATACTCGCCGATATTACTATCC
AAAATTCGATAAATTTGATGTCGATCACGATGGAAATCTTATAATTTTGT
CTCCTCAGATTGATGATGAAGGATTGAATTCCCAAATTTCAAAATTAAGT
TATGAATTCAATTCCAGGCAATACTATATTACATTAGGGGACCAACAGTC
TAAATATACCCCTCTTTATGTCGCTGCACATGGAACCTCAAACAGAACTT
GGGTTAGTCTGAAAAAGCAATCAGATGATCAGAAATGCCCAGAATACCGG
TTAATGTGTTTTAGATGTTGTATTAATAAAACGAATGGCGATAAAAGCCA
ACTCCGCGCAGCTCTGGAATCAAGTAGATGGAGAATGGCCGGCTTCAGTT
TGGGACCCTTGGTAGTTTTAGATGATCAAATTTTAGCTATAGATGAAGAA
CTTCACAATTTATGTTACATTTCATGGAGTGAAAATGAAGGTCATCCTAT
AGTTAAAAAAATATCATCACCAAATTCTCCTAATCTGATTGGCATGTGTT
CCGACGGACGAGATTATGCGTATTTATTTACTGAACACAACGTATTCAGG
TTTCGATTTCAAGAGCAGTTGATTAAAGATTCAGAACTTTTGGTTTGAAA
TAAAATATTTAATCCGTCATAATATTTATTTCTAAAATTTTATTTGAATA
CTTTTCTGTTTATTTATTCCATTTTTAAAACCGGCATCACTGAAAACAAG
AAGAGGATATTTTTATTCATCACAAAGGATTAGGCGTCCCGAGTTATTTA
TGAATTTTGGTAAAGATGTAAATGAAAATTGTAAAACGATTTGTAAGAAA
CGCAATCTGTCATCTTCCAATTCCAATAATTTAACTGAGCAAAGTAGTAA
AGATACTTCTTTTCAAATTTTTTCTCTTGATAAAATACTTATTGAAAAAT
CGATTCATACAGAAAGAATTTATGGAATAAATCCTTTGAAAACAATTCAT
GATCAATGTGTCAATCTTAATAGAATTGGAGTTCATATTCGTTTATTTAC
CATGGTCTTTTACAATTTCATTTTTATGTATTCCTTCAATGCATATAAAG
TGACAACATCGTATGTCATGTTACTCATTGTAGCTTTGGGTTTTTTCGTG
AAAGGAATATTTAGATTATTTTATTTGAAAAAATTTCCAACTAAAATCTT
TATATTATTTATTAGTATTTATATTTCTCAAATTCTTAATTACATGGTAG
TGCCGGGATTGGAAAAAGTAACATTAGCTGGAGTATTAAGTTAACTGAAC
TTTCTTTAACGAAAATATTAAAATGGAAAAAATTGTGCGAAC
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protein sequence of SMED30014307-orf-1

>SMED30014307-orf-1 ID=SMED30014307-orf-1|Name=SMED30014307-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=396bp
MNQEINYQPMYQNSEIQSANKSVTPVKKRNEKGNFKDRFSKIVSVYVPND
DAEKVLLKFDDEIEPETHDHYPKTLLQSLDNGLCYQVELVGSWQNFLGRK
AGKVQCSSVFRSTMFVVFENESQIFRMTLSENGISNDSIWFPKFQDSSIY
PIEIKCSNEVIYVSGKINTSEDVTQYLIVKYNMDENVLAYTRRYYYPKFD
KFDVDHDGNLIILSPQIDDEGLNSQISKLSYEFNSRQYYITLGDQQSKYT
PLYVAAHGTSNRTWVSLKKQSDDQKCPEYRLMCFRCCINKTNGDKSQLRA
ALESSRWRMAGFSLGPLVVLDDQILAIDEELHNLCYISWSENEGHPIVKK
ISSPNSPNLIGMCSDGRDYAYLFTEHNVFRFRFQEQLIKDSELLV*
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protein sequence of SMED30014307-orf-2

>SMED30014307-orf-2 ID=SMED30014307-orf-2|Name=SMED30014307-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=165bp
MNFGKDVNENCKTICKKRNLSSSNSNNLTEQSSKDTSFQIFSLDKILIEK
SIHTERIYGINPLKTIHDQCVNLNRIGVHIRLFTMVFYNFIFMYSFNAYK
VTTSYVMLLIVALGFFVKGIFRLFYLKKFPTKIFILFISIYISQILNYMV
VPGLEKVTLAGVLS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0003116parenchymal cell