Glutamate--cysteine ligase regulatory subunit-like

Overview


Name	Glutamate--cysteine ligase regulatory subunit-like
Smed ID	SMED30014306
Length (bp)	936

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Glutamate--cysteine ligase regulatory subunit-like (SMED30014306) t-SNE clustered cells

Violin plots show distribution of expression levels for Glutamate--cysteine ligase regulatory subunit-like (SMED30014306) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glutamate--cysteine ligase regulatory subunit-like (SMED30014306) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30014306

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
Smed sexual biotype	SMED30014306	h1SMcG0014301	Contig41432	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30014306	h1SMcG0014301	Contig41432	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
pharynx	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30014306	h1SMcG0014301	dd_Smed_v4_7012_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017
epidermis	SMED30014306	h1SMcG0014301	dd_Smed_v4_9290_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Human
Match: GCLM (glutamate-cysteine ligase modifier subunit [Source:HGNC Symbol;Acc:HGNC:4312])

HSP 1 Score: 76.6406 bits (187), Expect = 1.036e-15
Identity = 43/147 (29.25%), Postives = 76/147 (51.70%), Query Frame = 3

Query:  399 ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + +I + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P    + W P W+++ S I +SR +IKS  Y +  K
Sbjct:  102 SVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAK 248

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Human
Match: GCLM (glutamate-cysteine ligase modifier subunit [Source:HGNC Symbol;Acc:HGNC:4312])

HSP 1 Score: 77.0258 bits (188), Expect = 1.234e-15
Identity = 43/147 (29.25%), Postives = 76/147 (51.70%), Query Frame = 3

Query:  399 ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + +I + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P    + W P W+++ S I +SR +IKS  Y +  K
Sbjct:  124 SVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Celegans
Match: E01A2.1 (pep chromosome:WBcel235:I:4140649:4142367:1 gene:WBGene00017084.1 transcript:E01A2.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E01A2.1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.605e-14
Identity = 56/243 (23.05%), Postives = 115/243 (47.33%), Query Frame = 3

Query:   90 MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF-EHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPK----DNEKHVD-----ISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYES 788
            +T   + T N+  +T     +FE++++++  C    ++   +   + F E+S  + C      NY +++  I+I +K+F+ ++ ++ +++A   + +         +IL+ P+    D E   D       ++ PL+ ++   +   +I  +G++D +  QLK       V PSIN       C V PEL+  AN + ++LL HND     +P   N +  +    +  + K V  CS ++E+
Sbjct:   14 LTRFRLHTGNVNGYTQLKVRRFETSAEELAACLD--LQLSSNPIADDFKENSCLLLCDNDHIDNYERDE--IKITMKVFLSAWDVQQIDQAVTSLKEQLKTKDIEVLILSFPELDLIDGESEEDENNRWFEKVKPLYTYMEKLVETSEIASIGVSDFSARQLKTIIDHFDVKPSINHVRLDGCCQVPPELQALANDNDVQLLVHNDP----TPFPTNNIFKTFCEIDSGSQKAV--CSPLFET 246

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Celegans
Match: E01A2.1 (pep chromosome:WBcel235:I:4139514:4142335:1 gene:WBGene00017084.1 transcript:E01A2.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:E01A2.1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.601e-14
Identity = 56/243 (23.05%), Postives = 115/243 (47.33%), Query Frame = 3

Query:   90 MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF-EHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPK----DNEKHVD-----ISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYES 788
            +T   + T N+  +T     +FE++++++  C    ++   +   + F E+S  + C      NY +++  I+I +K+F+ ++ ++ +++A   + +         +IL+ P+    D E   D       ++ PL+ ++   +   +I  +G++D +  QLK       V PSIN       C V PEL+  AN + ++LL HND     +P   N +  +    +  + K V  CS ++E+
Sbjct:   28 LTRFRLHTGNVNGYTQLKVRRFETSAEELAACLD--LQLSSNPIADDFKENSCLLLCDNDHIDNYERDE--IKITMKVFLSAWDVQQIDQAVTSLKEQLKTKDIEVLILSFPELDLIDGESEEDENNRWFEKVKPLYTYMEKLVETSEIASIGVSDFSARQLKTIIDHFDVKPSINHVRLDGCCQVPPELQALANDNDVQLLVHNDP----TPFPTNNIFKTFCEIDSGSQKAV--CSPLFET 260

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Fly
Match: Gclm (gene:FBgn0046114 transcript:FBtr0334605)

HSP 1 Score: 59.6918 bits (143), Expect = 5.991e-10
Identity = 35/128 (27.34%), Postives = 64/128 (50.00%), Query Frame = 3

Query:  444 DISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISV 827
             +++L  L+  L     + +I +LGIADL+ A L+E   +  V+P+I Q   S  C V PEL++F   H I+L +H+D   ++  E+ + ++        +T  W ++       R V+ +  Y +  
Sbjct:  159 GVAELKELYKTLEQYALKQQITQLGIADLDAAALEELHNSAQVVPTIAQVNLSTCCVVPPELQEFCTAHDIQLNTHSDPELLLPVEQFDGLVPG------YTIDWTLRYQVHVRCRGVLTAKGYIVGA 280

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Fly
Match: Gclm (gene:FBgn0046114 transcript:FBtr0084336)

HSP 1 Score: 59.6918 bits (143), Expect = 5.991e-10
Identity = 35/128 (27.34%), Postives = 64/128 (50.00%), Query Frame = 3

Query:  444 DISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISV 827
             +++L  L+  L     + +I +LGIADL+ A L+E   +  V+P+I Q   S  C V PEL++F   H I+L +H+D   ++  E+ + ++        +T  W ++       R V+ +  Y +  
Sbjct:  159 GVAELKELYKTLEQYALKQQITQLGIADLDAAALEELHNSAQVVPTIAQVNLSTCCVVPPELQEFCTAHDIQLNTHSDPELLLPVEQFDGLVPG------YTIDWTLRYQVHVRCRGVLTAKGYIVGA 280

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Zebrafish
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:ZFIN;Acc:ZDB-GENE-030131-5906])

HSP 1 Score: 78.9518 bits (193), Expect = 1.351e-16
Identity = 61/258 (23.64%), Postives = 116/258 (44.96%), Query Frame = 3

Query:   93 TNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEHST----DIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVD-ISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNS---ELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK*SFN 842
            T L + T N++  +   K    S S+++ +C +  +  W  +   S +       D    ++ +    +   ++++ VK+F+  +    +  A +    +       +VI+  P   E     ++ L PLW  L S +   KI  +G +DL+   L++      + PS NQ   ++ C + P+L  FA    I+LL+H+D   ++S       +     EL  + W  +WV++ S I +SR +IK+  Y +  K S N
Sbjct:   15 TTLKLHTGNLVNRSRLKKKCPSSPSEELQDCIQGTLSEWFTAMPSSLDSELPSVLDCSIPENTEAITPEEREELKVSVKLFLTEWDCSSIRSAVDMACLSLGVSQLDSVIIAPPSLPEGESQTLTHLQPLWQELESLVQSQKIAAIGTSDLDKTLLEQLYNWAQIKPSSNQVNLASCCVMPPDLTAFAKEFDIQLLTHSDPKELISAAGFQEAVQGSSQELQVDDWRLEWVLRYSIIVKSRGIIKAKGYIVHAKKSNN 272

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Xenopus
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:NCBI gene;Acc:549290])

HSP 1 Score: 82.0333 bits (201), Expect = 1.864e-17
Identity = 61/252 (24.21%), Postives = 117/252 (46.43%), Query Frame = 3

Query:   99 LTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCW--KDSNQESFEHSTDIKCMKHRDKNYLKNDL--DIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPK-DNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLN---SELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            L I T N++ W    K    + S+++ +C R  +  W  K S +   E    ++C   +    +  D   ++++ VK+F+ S     + +A +            ++I+  P  ++ +   +  L P W  L S +   K+  +G +DL+ A L++      V P+ NQ   ++ C + P+L +FA    I+LL+HND   ++S E     L     E   + W+P W+++ S I ++R +IK   Y +  +
Sbjct:   18 LVIQTGNLLNWGCLKKKCPSTPSEELQDCIRTTLNEWSQKFSPELVKEIPQTLECTVPQAMETINLDEREEMKVSVKLFIVSPSHSSVTQAIDMACSTLGVAQIDSMIIAPPPLEDGRSFSLENLQPYWEELESLVRNGKVVSIGTSDLDKALLEQLYLWSQVKPASNQVNLASCCIMPPDLTEFAKQFDIQLLTHNDPKELLSEEAFQEALKESAQECHSSAWSPIWILRYSVIVKTRGIIKLKGYILQAQ 269

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Xenopus
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:NCBI gene;Acc:549290])

HSP 1 Score: 74.3294 bits (181), Expect = 6.527e-15
Identity = 62/249 (24.90%), Postives = 112/249 (44.98%), Query Frame = 3

Query:   99 LTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCW--KDSNQESFEHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLN---SELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            L I T N++ W    K    + S+++ +C R  +  W  K S +   E    ++C   +    +  +LD R  +K+            A   +    ID   + +I   P ++ +   +  L P W  L S +   K+  +G +DL+ A L++      V P+ NQ   ++ C + P+L +FA    I+LL+HND   ++S E     L     E   + W+P W+++ S I ++R +IK   Y +  +
Sbjct:   18 LVIQTGNLLNWGCLKKKCPSTPSEELQDCIRTTLNEWSQKFSPELVKEIPQTLECTVPQAMETI--NLDEREEMKV-----------SACSTLGVAQID---SMIIAPPPLEDGRSFSLENLQPYWEELESLVRNGKVVSIGTSDLDKALLEQLYLWSQVKPASNQVNLASCCIMPPDLTEFAKQFDIQLLTHNDPKELLSEEAFQEALKESAQECHSSAWSPIWILRYSVIVKTRGIIKLKGYILQAQ 250

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Mouse
Match: Gclm (glutamate-cysteine ligase, modifier subunit [Source:MGI Symbol;Acc:MGI:104995])

HSP 1 Score: 77.0258 bits (188), Expect = 7.386e-16
Identity = 57/255 (22.35%), Postives = 117/255 (45.88%), Query Frame = 3

Query:   93 TNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKC-MKHR-DKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTF--ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + L + T N++ W    K    ++S+++ +C +  +  W          E    ++C M H  +K       ++++  K+F+          +   ++ + + +    + ++ + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P      W P W+++ S I +SR +IKS  Y +  K
Sbjct:   16 STLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVLECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

Query:   93 TNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKC-MKHR-DKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTF--ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + L + T N++ W    K    ++S+++ +C +  +  W          E    ++C M H  +K       ++++  K+F+          +   ++ + + +    + ++ + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P      W P W+++ S I +SR +IKS  Y +  K
Sbjct:   16 STLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVLECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Mouse
Match: Gclm (glutamate-cysteine ligase, modifier subunit [Source:MGI Symbol;Acc:MGI:104995])

HSP 1 Score: 53.5286 bits (127), Expect = 4.978e-8
Identity = 27/93 (29.03%), Postives = 51/93 (54.84%), Query Frame = 3

Query:  399 ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHND 677
            + ++ + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND
Sbjct:   93 SVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHND 185

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
Match: sp|Q2T9Y6|GSH0_BOVIN (Glutamate--cysteine ligase regulatory subunit OS=Bos taurus OX=9913 GN=GCLM PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.228e-15
Identity = 59/258 (22.87%), Postives = 117/258 (45.35%), Query Frame = 3

Query:   93 TNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKCMKHRDKNYLKNDL--DIRIIVKIFMY-----SYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + L + T N++ W    K    ++S+++ +C +  +  W          E    ++C        +  D   ++++  K+F+      S     ++ A   +    +D   + +I + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P    + W P W+++ S I +SR +IKS  Y +  K
Sbjct:   16 STLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLIREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLD---SVIIASPPVEDGVNLSLEHLQPYWEELQNLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIRAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
Match: sp|P48508|GSH0_RAT (Glutamate--cysteine ligase regulatory subunit OS=Rattus norvegicus OX=10116 GN=Gclm PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 4.248e-15
Identity = 60/258 (23.26%), Postives = 118/258 (45.74%), Query Frame = 3

Query:   93 TNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKC-MKHR-DKNYLKNDLDIRIIVKIFMY-----SYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + L + T N++ W    K    ++S+++ +C +  +  W          E    ++C M H  +K       ++++  K+F+      S     ++ A   +    +D   + ++ + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P      W P W+++ S I +SR +IKS  Y +  K
Sbjct:   16 STLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVLECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLD---SVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQEWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
Match: sp|O09172|GSH0_MOUSE (Glutamate--cysteine ligase regulatory subunit OS=Mus musculus OX=10090 GN=Gclm PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.166e-15
Identity = 57/255 (22.35%), Postives = 117/255 (45.88%), Query Frame = 3

Query:   93 TNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKC-MKHR-DKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTF--ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + L + T N++ W    K    ++S+++ +C +  +  W          E    ++C M H  +K       ++++  K+F+          +   ++ + + +    + ++ + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P      W P W+++ S I +SR +IKS  Y +  K
Sbjct:   16 STLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVLECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
Match: sp|P48507|GSH0_HUMAN (Glutamate--cysteine ligase regulatory subunit OS=Homo sapiens OX=9606 GN=GCLM PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.925e-15
Identity = 43/147 (29.25%), Postives = 76/147 (51.70%), Query Frame = 3

Query:  399 ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            + +I + P ++  ++ +  L P W  L + +   KI  +G +DL+  QL++  +   V P+ NQ   ++ C + P+L  FA    I+LL+HND   ++S       L   +P    + W P W+++ S I +SR +IKS  Y +  K
Sbjct:  124 SVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAK 270

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
Match: sp|O94246|GSH0_SCHPO (Putative glutamate--cysteine ligase regulatory subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC737.06c PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 4.925e-7
Identity = 45/202 (22.28%), Postives = 92/202 (45.54%), Query Frame = 3

Query:  303 RIIVKIFMYSYCLEDLEEAFEYISKNFIDLTF-------ATVILNVP-----------KDNEKHVDISQLLP--------LWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLN---SELPFNCWTPKWVVKCSKIYESRSVIKSLEYAI 821
             IIVK+F       D+++  E +S+ F +L         +T++++ P             NE +  I ++ P         W  L  K+ + KI  LG+++  + +L+    +V V+P   Q      C +  +L +FA+ H ++L  H+D + ++S  ++  +++    E+P N     WV++ + +    +VI    Y +
Sbjct:   81 EIIVKLFFLDGENIDIKKREETLSQVFYNLHMLFGIDFVSTLVVSFPHITFLKESGNSSSNEIYDSIDEIPPQEIQSWVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQINIGQNCKLPNDLLNFADRHHLKLFFHSDPSALLSESEITSVIHKACPEIP-NPARVDWVIRYTILTRHTAVIHQKGYIV 281

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
Match: K1Q9C8 (Glutamate--cysteine ligase regulatory subunit OS=Crassostrea gigas OX=29159 GN=CGI_10014941 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.960e-23
Identity = 71/254 (27.95%), Postives = 131/254 (51.57%), Query Frame = 3

Query:   90 MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDS-NQESFEHSTDIKCMKHRDKNYLKND--LDIRIIVKIFM-----YSYCLEDLEEAFEYISKNFIDLTFATVILNVPK-DNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVML---NSELPFNCWTPKWVVKCSKIYESRSVIKSLEY 815
            +T+LT+ + NI+ W    +      + +I EC    +K + DS ++ + ++ T ++C   +    L  D   D+++ VK+F+      S   E +E+    ++  FI+    TVIL +P+ + ++ V +S + P W  L   +   ++  LGIADLN  QL+E      + P INQ    + C +  +L ++A  + I+LLSHND   ++  EKL+ ++    +E   + W   WV++ S + + R +IK+  Y
Sbjct:   11 VTSLTVHSGNIVNWNRLKRHPNHGATAEIAECIDSALKTYLDSADKTALQYETSLECKSTKFMEALPADERGDLKVTVKVFLCKLLPPSTLKEGIEKVLTELNITFIE----TVILALPELEEDEEVSLSVIQPYWEALEEMVEGERVLSLGIADLNKEQLEELYNWAKIKPGINQVNLESCCVMPKDLTEYAKQNDIQLLSHNDARDILPNEKLSEIIKTNTTEKDSDFWEMLWVMRYSVLIKCRGIIKTKAY 260

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
Match: A0A564Y432 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS2691 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 9.828e-23
Identity = 72/267 (26.97%), Postives = 130/267 (48.69%), Query Frame = 3

Query:   99 LTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYS----------------YCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQ-----LLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSEL---PFNCWTPKWVVKCSKIYESRSVIKSLEYAI 821
            L + T+NI++W N   IK  +  D++  C + V++CW   N+ +   E    I C KHRDK        + +  K+F+++                  LED+E     +S    +L+   ++ + P        I Q     LL +W  L   +  +KI+RLG+ D+ +++++    AV + P +NQ    + C +  EL++FA  H I+LL+H+D   ++  EKL  ++ +E        W P W+V  ++ Y++R V++S  Y +
Sbjct:   10 LFLHTSNILKWNNVQLIK--NPFDEVETCIKTVMQCWHSCNKTNLKDEEYLCIACDKHRDKIPDWERSSVTVSAKLFLFTDDHDAIRQAVNKTLDRLGLEDMETLVVSLSP---ELSRGAIVDHPPCLGTTWARIPQNTAHSLLKIWPVLEEFVETNKIFRLGVRDMPVSEMEALYTAVKIPPKVNQVFIGSTCLMSEELENFAKQHDIQLLTHHDSADMLPEEKLQSVMRNEFCDEDGAGWRPSWLVSYAEYYKNRGVVRSKGYTL 271

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
Match: A0A068WDE9 (Glutamate cysteine ligase regulatory OS=Echinococcus granulosus OX=6210 GN=EgrG_000887650 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 4.896e-22
Identity = 73/286 (25.52%), Postives = 133/286 (46.50%), Query Frame = 3

Query:  111 TNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYS----------------YCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQ-----LLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFN---CWTPKWVVKCSKIYESRSVIKSLEYAISVK*SFNCD--KNGNLANSTAQA 884
            T+NI++W N   I+  +  +++  C R V+ CW          + +  I C KHRDK       ++ +  K+F+++                  LEDLE     +S    +L+   ++ + P        I Q     LL +W  L   +  +KI+RLG++D+ +  L+    AV + P +NQ    + C +  EL++FA  H I+LL+H+D   ++  EKL  ++ +E        W P W+++ ++ Y++R V++S  Y +  +          G+ A S+A  
Sbjct:   14 TSNILKWNNMQLIR--NPFEEVETCIRTVMHCWHSRRNADLCEQETLCIACDKHRDKIPESERSNVIVSAKLFLFTDDHDSIRQAVNKTLDRLGLEDLETLIISLSP---ELSRGALVDHPPCMGLPWARIPQNTAHSLLKIWPVLEEFVEGNKIFRLGVSDMPVTDLEALCAAVKIPPKVNQVFIGSTCLLGDELENFAKLHSIQLLTHHDSADMLPEEKLQAVMRNEFSAEDAAGWRPSWLMRYAEYYKNRGVVRSRGYTLFAERDLPASMATTGDAATSSANG 294

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
Match: A0A068YG87 (Glutamate cysteine ligase regulatory subunit OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000887650 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 5.312e-22
Identity = 73/286 (25.52%), Postives = 133/286 (46.50%), Query Frame = 3

Query:  111 TNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESF--EHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYS----------------YCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQ-----LLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFN---CWTPKWVVKCSKIYESRSVIKSLEYAISVK*SFNCD--KNGNLANSTAQA 884
            T+NI++W N   I+  +  +++  C R V+ CW          + +  I C KHRDK       ++ +  K+F+++                  LEDLE     +S    +L+   ++ + P        I Q     LL +W  L   +  +KI+RLG++D+ +  L+    AV + P +NQ    + C +  EL++FA  H I+LL+H+D   ++  EKL  ++ +E        W P W+++ ++ Y++R V++S  Y +  +          G+ A S+A  
Sbjct:   14 TSNILKWNNMQLIR--NPFEEVETCIRTVMHCWHSRRNADLCEQETLCIACDKHRDKIPESERSNVIVSAKLFLFTDDHDSIRQAVNKTLDRLGLEDLETLIISLSP---ELSRGALVDHPPCMGLPWARIPQNTAHSLLKIWPVLEEFVEGNKIFRLGVSDMPVTDLEALCAAVKIPPKVNQVFIGSTCLLGDELENFAKLHSIQLLTHHDSADMLPEEKLQAVMRNEFSAEDAVGWRPSWLMRYAEYYKNRGVVRSRGYTLFAERDLPASMANTGDAATSSANG 294

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
Match: T1J7Y0 (Aldo_ket_red domain-containing protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 5.323e-22
Identity = 65/248 (26.21%), Postives = 122/248 (49.19%), Query Frame = 3

Query:   78 FEILMTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCW-KDSNQESFEHSTDIKCMKHRDKNYLKNDL--DIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPF---NCWTPKWVVKCSKIYESRSVIK 803
            F   + +LT+ T NII W    +   +S +D+++E     +  W ++ + E +   T+I C+ H+  + +   +  D+++ VKI +     E L EA   + +        +VI++ P    + +++  + PLWN L   I   +I  +G++DL+   L       T+ PSINQ   ++ C +  E+  FA  + I+LL+HND   ++  +  + +L S   F     W P W+ + S + + R VI+
Sbjct:   44 FPDYVHSLTVHTGNIIHWNELKRKTSQSATDELVETLGETLSTWSRNYDPEQYIGLTNIDCINHKYDDPVDKQMRKDLKVTVKILLCQLDPEGLSEAVTKVLQELRVDFLESVIVSFPSPPNEPLELHHIKPLWNVLERYIDSGQIVTIGVSDLDTELLIALYAWATIKPSINQVNLASCCVMPQEMTAFAKENDIQLLTHNDPKDILPLDVCSGILRSSGCFLDSEAWAPLWIARYSVLVKCRGVIQ 291

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Cavefish
Match: gclm (glutamate-cysteine ligase modifier subunit [Source:NCBI gene;Acc:103026947])

HSP 1 Score: 72.7886 bits (177), Expect = 1.724e-14
Identity = 42/144 (29.17%), Postives = 73/144 (50.69%), Query Frame = 3

Query:  399 ATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVML---NSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAI 821
            + +I   P    + + ++ L PLW  L   +   K+  +G +DL+ A L++      V PS NQ   ++ C + P+L  FA    I+LL+HND   ++S E     +   + +L    W  +WV++ S I +SR +IK+  Y +
Sbjct:  121 SVIIAPPPLPEGETLALAHLQPLWEELEGLVRSQKVAAIGTSDLDKALLEQLYNWAQVKPSSNQVNLASCCVMPPDLTAFAKEFDIQLLTHNDSKEMISAEGFKEAVQESSKDLQVADWRLEWVLRYSIIVKSRGIIKAKGYLV 264

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Sea Lamprey
Match: gclm (glutamate-cysteine ligase, modifier subunit [Source:ZFIN;Acc:ZDB-GENE-030131-5906])

HSP 1 Score: 102.064 bits (253), Expect = 1.312e-25
Identity = 68/255 (26.67%), Postives = 117/255 (45.88%), Query Frame = 3

Query:   96 NLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEHSTDIKCMKH-------RDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNF-IDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP--FNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
             LT+ T N++           S S+++++C R  +  W  S  + F       C+          +K   ++  D++I  KIF+     + ++ A E       + L  A V+   P   E+ +   +L P W  L S I+ HK+  +G +DL+   L+E      V P+ NQ   ++ C + PEL  FA  + I+LL+HND   ++  +    M+ +  P     WTP WV++ S I + R V+KS  Y +  +
Sbjct:   21 TLTVHTGNVVNRGALRNKYGSSPSEELVDCVRSTLSDWA-SKLDGFARDQSPSCLAQDVCVCPGTEKISAEDRDDLKITAKIFLLGGDAQVIKPAVEKACMALGVSLLDAVVLAPPPLPEEEKLTADRLSPYWAELESLIAAHKVATIGTSDLDSQLLEELYHWAQVKPASNQVNLASCCVMPPELTAFAKEYDIQLLTHNDPTEILPTKSFQDMVATVAPEQAEVWTPLWVLRYSVIIKCRGVLKSKGYFLQAR 274

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Nematostella
Match: EDO44307 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWF3])

HSP 1 Score: 61.6178 bits (148), Expect = 4.643e-11
Identity = 59/247 (23.89%), Postives = 110/247 (44.53%), Query Frame = 3

Query:  105 ILTNNIIQWTNTNKIKFESNSDKII-ECFRCVIKCWKDSNQESFEHSTDIKCMKH-----RDKNYLKNDLDIRIIVKIFMYSYCLEDLEEA-FEYISKNFIDLTFATVILNVP--KDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKD-FANHHGIELLSHNDDNHVVSPEKLNVMLN---SELPFNCWTPKWVVKCSKIYESRSVIKS 806
            I   NI  W+ T +     +SD++  E         + +      H   +K ++       +K  L   + ++I  KIF  S     + ++ F  + +  +D +  T+IL +P  +D E+H   + +   W  +        +  LG+ DL+   L++      + P I+Q    A+C V+P+    ++    I+LL+H D   ++S E+L  +L     E+    W P WV++ S + + R VIKS
Sbjct:   23 IHGGNISNWSRTKRKTTADSSDEVSSETLVRAQDIHRATIPPPLPHRVSVKTVRFVNDCFMEKIKLDERMHLKISAKIFAISLNPAVVRDSLFRAMLELGVD-SIETLILTLPDIQDPEQH--FTTIKSFWQEMEQLYDSGYVQTLGVCDLDKPTLEKLYDWARIKPEIDQVNL-ASCCVMPKASSTYSKTRDIQLLTHADPREMLSEERLQQVLGVVSEEMNVAGWRPSWVMRYSVLVKCRGVIKS 265

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Medaka
Match: gclm (glutamate-cysteine ligase modifier subunit [Source:NCBI gene;Acc:101162516])

HSP 1 Score: 81.6481 bits (200), Expect = 1.538e-17
Identity = 59/246 (23.98%), Postives = 110/246 (44.72%), Query Frame = 3

Query:  111 TNNIIQWTNTNKIKFESNSDKIIECFRCVIKCW---KDSNQESFEHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEK-LNVMLNS--ELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            T N++  +   K    S S+++ +C +  +  W   K +    F    D    +  +    +   ++++ VK+F+       + +A +   +        +VI+  P   E    ++ L P W  L + +   KI  +G +DL+   L++      V PS NQ   ++ C + P+L  FA    I+LL+H+D   ++S E  L  M     +   + W  +WV++ S I +SR + KS  Y +S K
Sbjct:   22 TGNLVNRSRLKKKCPGSTSEELRDCIQATMSDWVSMKKAASPGFPDVLDCSLPEATETITPEEREELKVSVKLFLCEAGQSAIGDAVDLACQTLAVSQLDSVIIAPPPPLEADQSLAHLQPAWEQLEALVRSQKIAAIGTSDLDKDLLEQLYNWAQVKPSSNQVNLASCCVMPPDLTAFAKDFDIQLLTHSDPKELMSAESFLEAMREGARDQSLSGWRLEWVLRYSVIVKSRGIFKSKGYLVSAK 267

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Medaka
Match: gclm (glutamate-cysteine ligase modifier subunit [Source:NCBI gene;Acc:101162516])

HSP 1 Score: 75.8702 bits (185), Expect = 1.712e-15
Identity = 49/181 (27.07%), Postives = 87/181 (48.07%), Query Frame = 3

Query:  297 DIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEK-LNVMLNS--ELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            ++++ VK+F+       + +A +   +        +VI+  P   E    ++ L P W  L + +   KI  +G +DL+   L++      V PS NQ   ++ C + P+L  FA    I+LL+H+D   ++S E  L  M     +   + W  +WV++ S I +SR + KS  Y +S K
Sbjct:   88 ELKVSVKLFLCEAGQSAIGDAVDLACQTLAVSQLDSVIIAPPPPLEADQSLAHLQPAWEQLEALVRSQKIAAIGTSDLDKDLLEQLYNWAQVKPSSNQVNLASCCVMPPDLTAFAKDFDIQLLTHSDPKELMSAESFLEAMREGARDQSLSGWRLEWVLRYSVIVKSRGIFKSKGYLVSAK 268

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Planmine SMEST
Match: SMESG000005952.1 (SMESG000005952.1)

HSP 1 Score: 496.508 bits (1277), Expect = 8.605e-180
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 3

Query:   90 MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 830
            MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDIS LLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQ+CS ACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK
Sbjct:    1 MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEHSTDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISLLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQSCSVACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK 247

[Close]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Planmine SMEST
Match: SMESG000053228.1 (SMESG000053228.1)

HSP 1 Score: 110.923 bits (276), Expect = 1.147e-28
Identity = 78/253 (30.83%), Postives = 133/253 (52.57%), Query Frame = 3

Query:   90 MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEHS----TDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLED----LEEAFEYISKNFIDLTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLAQLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHVVSPEKLNVMLNSELP---FNCWTPKWVVKCSKIYESRSVIKSLEY 815
            M  + I T+NII W +       +++D+I++C   V+KC+   N+  F ++     +++C+ H+D   L +  D+R+ VK+F+ +   ED    L + F+Y+  N +D   + +I   P+     + +  +  LW+ L   +  H+I +LG+ D  L QL++  ++V + PSINQ      CSV   LKDFA  H I+LLSH+D   ++        L   +P        P WV + S +Y++RSVI+S  Y
Sbjct:    1 MNEMLIHTSNIINWKSIKNNPQLTSTDEILKCSIQVLKCYM-KNENLFANNKLSDVEVECINHKDL-ILDDRSDLRVNVKLFLINNNYEDISKHLAQIFKYLECNELD---SLIISITPETICSSICMGDVYELWSKLEEYVKDHRILQLGVCDFTLEQLEDMWQSVEIKPSINQIYWKRMCSVSQSLKDFAADHHIQLLSHHDKPDILPSNLFKTRLQEIMPNIMIESVDPSWVCRYSIVYKNRSVIRSKGY 248

The following BLAST results are available for this feature:

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Human
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Celegans
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Fly
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Zebrafish
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Xenopus
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Mouse
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Cavefish
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Sea Lamprey
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Yeast
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Nematostella
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Medaka
BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Planmine SMEST

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
GCLM	1.036e-15	29.25	glutamate-cysteine ligase modifier subunit [Source...	[more]
GCLM	1.234e-15	29.25	glutamate-cysteine ligase modifier subunit [Source...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
E01A2.1	5.605e-14	23.05	pep chromosome:WBcel235:I:4140649:4142367:1 gene:W...	[more]
E01A2.1	6.601e-14	23.05	pep chromosome:WBcel235:I:4139514:4142335:1 gene:W...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Gclm	5.991e-10	27.34	gene:FBgn0046114 transcript:FBtr0334605	[more]
Gclm	5.991e-10	27.34	gene:FBgn0046114 transcript:FBtr0084336	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gclm	1.351e-16	23.64	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gclm	1.864e-17	24.21	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]
gclm	6.527e-15	24.90	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Gclm	7.386e-16	22.35	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]
Gclm	7.386e-16	22.35	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]
Gclm	4.978e-8	29.03	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q2T9Y6\|GSH0_BOVIN	3.228e-15	22.87	Glutamate--cysteine ligase regulatory subunit OS=B...	[more]
sp\|P48508\|GSH0_RAT	4.248e-15	23.26	Glutamate--cysteine ligase regulatory subunit OS=R...	[more]
sp\|O09172\|GSH0_MOUSE	5.166e-15	22.35	Glutamate--cysteine ligase regulatory subunit OS=M...	[more]
sp\|P48507\|GSH0_HUMAN	5.925e-15	29.25	Glutamate--cysteine ligase regulatory subunit OS=H...	[more]
sp\|O94246\|GSH0_SCHPO	4.925e-7	22.28	Putative glutamate--cysteine ligase regulatory sub...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
K1Q9C8	1.960e-23	27.95	Glutamate--cysteine ligase regulatory subunit OS=C...	[more]
A0A564Y432	9.828e-23	26.97	Uncharacterized protein OS=Hymenolepis diminuta OX...	[more]
A0A068WDE9	4.896e-22	25.52	Glutamate cysteine ligase regulatory OS=Echinococc...	[more]
A0A068YG87	5.312e-22	25.52	Glutamate cysteine ligase regulatory subunit OS=Ec...	[more]
T1J7Y0	5.323e-22	26.21	Aldo_ket_red domain-containing protein OS=Strigami...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gclm	1.724e-14	29.17	glutamate-cysteine ligase modifier subunit [Source...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
gclm	1.312e-25	26.67	glutamate-cysteine ligase, modifier subunit [Sourc...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO44307	4.643e-11	23.89	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
gclm	1.538e-17	23.98	glutamate-cysteine ligase modifier subunit [Source...	[more]
gclm	1.712e-15	27.07	glutamate-cysteine ligase modifier subunit [Source...	[more]

BLAST of Glutamate--cysteine ligase regulatory subunit-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000005952.1	8.605e-180	98.79	SMESG000005952.1	[more]
SMESG000053228.1	1.147e-28	30.83	SMESG000053228.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30014306 ID=SMED30014306|Name=Glutamate--cysteine ligase regulatory subunit-like|organism=Schmidtea mediterranea sexual|type=transcript|length=936bp
CTGACATTTATAATTTATCAACTTGATTTAAAGTTAATATTTTAAAAACA
GTTGAAATTAATTTATTTAAGTGTTTATTTGAAATTTTAATGACAAATTT
AACAATTTTAACTAACAACATAATTCAGTGGACAAATACTAATAAAATCA
AATTCGAAAGTAATTCTGACAAAATTATTGAATGTTTCAGATGCGTAATA
AAGTGCTGGAAAGATTCAAACCAGGAATCTTTTGAACACTCCACTGACAT
CAAGTGCATGAAACACAGAGACAAAAATTATTTGAAAAACGACTTAGATA
TCCGCATAATAGTAAAAATATTCATGTATTCTTACTGCCTTGAAGATCTC
GAAGAAGCTTTTGAATATATTTCCAAAAACTTCATTGATTTGACCTTTGC
GACAGTAATTTTAAATGTTCCCAAGGACAATGAAAAACACGTAGATATTT
CACAGTTGTTACCATTATGGAACTTTCTATCCAGCAAAATTTCACAACAC
AAAATTTGGCGGCTAGGAATAGCTGATTTGAATTTAGCACAGCTAAAAGA
ATTTACTAAAGCTGTTACGGTTCTACCAAGTATAAATCAACAAGCTTGCT
CAGCTGCCTGTTCAGTTATTCCAGAATTGAAAGATTTTGCCAACCATCAC
GGAATTGAGCTTTTGTCTCATAATGATGATAATCATGTGGTCTCCCCAGA
GAAGTTAAATGTTATGCTAAACTCTGAATTACCTTTCAATTGTTGGACTC
CAAAATGGGTCGTAAAATGTTCAAAAATCTATGAAAGTCGGAGCGTTATC
AAGTCGCTGGAATATGCGATTTCCGTCAAATAAAGTTTTAACTGTGATAA
AAATGGAAATCTAGCGAATTCTACAGCACAAGCTTTTATAATCTTTACTT
TTTCATTTTATAATTAAATTAAATTTTTAATTAAAA

protein sequence of SMED30014306-orf-1

>SMED30014306-orf-1 ID=SMED30014306-orf-1|Name=SMED30014306-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=248bp
MTNLTILTNNIIQWTNTNKIKFESNSDKIIECFRCVIKCWKDSNQESFEH
STDIKCMKHRDKNYLKNDLDIRIIVKIFMYSYCLEDLEEAFEYISKNFID
LTFATVILNVPKDNEKHVDISQLLPLWNFLSSKISQHKIWRLGIADLNLA
QLKEFTKAVTVLPSINQQACSAACSVIPELKDFANHHGIELLSHNDDNHV
VSPEKLNVMLNSELPFNCWTPKWVVKCSKIYESRSVIKSLEYAISVK*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000034	epidermis

Vocabulary: **INTERPRO**
Term	Definition
IPR036812	NADP_OxRdtase_dom_sf
IPR032963	Gclm
IPR023210	NADP_OxRdtase_dom

Vocabulary: **biological process**
Term	Definition
GO:0006750	glutathione biosynthetic process

Vocabulary: **molecular function**
Term	Definition
GO:0016874	ligase activity
GO:0035226	glutamate-cysteine ligase catalytic subunit binding

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036812	NADP-dependent oxidoreductase domain superfamily	GENE3D	G3DSA:3.20.20.100		coord: 67..209 e-value: 5.8E-6 score: 27.1
IPR036812	NADP-dependent oxidoreductase domain superfamily	SUPERFAMILY	SSF51430	NAD(P)-linked oxidoreductase	coord: 70..193
IPR032963	Glutamate--cysteine ligase regulatory subunit	PANTHER	PTHR13295	GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT	coord: 2..246

Main menu

Glutamate--cysteine ligase regulatory subunit-like

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 11

Owlery

blazegraph-runner