G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30014302
Length (bp)414
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014302

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30014302SMESG000042531.1 dd_Smed_v4_58155_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014302SMESG000042531.1 dd_Smed_v4_58155_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014302SMESG000042531.1 dd_Smed_v4_58155_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014302SMESG000042531.1 dd_Smed_v4_61185_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014302SMESG000042531.1 dd_Smed_v4_61185_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014302SMESG000042531.1 dd_Smed_v4_61185_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014302SMESG000042531.1 dd_Smed_v6_61185_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3UHX3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS6979 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.495e-17
Identity = 52/149 (34.90%), Postives = 76/149 (51.01%), Query Frame = 2 
Query:    2 EKLALFECRPQLDSGLW-KFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKAL------RNNQKL-------------------------KIKNSDASSGLESQIKPIVKRN------KKRRTEHIVKVLTALSLIHAITTLPAAIY 334
            + + +F C P  D   W + F K+DFI++AILPYVILF CN+YI+  L++Q K L      R  QK+                         + ++  ASS   SQ K I+         KKR+ E ++KVLTAL+++H + TLP  I+
Sbjct:  166 QNVTVFVCTPDEDYTKWTRLFMKIDFISFAILPYVILFSCNLYIFCVLRRQRKLLSAAINQRQRQKVLLVANLGSDSLTLPTTSPDIEESGSRTRDFRASSRCGSQAKSILGSGSSKPERKKRKPEDVIKVLTALTVVHVVCTLPGTIF 314
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267EZ28 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017324g1 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.640e-15
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 2 
Query:   26 RPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNK-------KRRTEHIVKVLTALSLIHAITTLPAAIYQAM 343
            R   D    ++F KLD +TY  +PY IL FCNIYIY  +++Q  A++ ++  K  N+               RNK       +R+ E ++K+L  L++IH ITTLPA+I Q +
Sbjct:  219 RCSYDKAFGQWFAKLDLVTYLFIPYTILLFCNIYIYAAIRRQAAAVKQHKSKKTSNN---------------RNKQDGGGGGRRKPESVMKILAVLTVIHIITTLPASITQVL 316
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267E7V2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017324g2 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.865e-15
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 2 
Query:   26 RPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNK-------KRRTEHIVKVLTALSLIHAITTLPAAIYQAM 343
            R   D    ++F KLD +TY  +PY IL FCNIYIY  +++Q  A++ ++  K  N+               RNK       +R+ E ++K+L  L++IH ITTLPA+I Q +
Sbjct:  219 RCSYDKAFGQWFAKLDLVTYLFIPYTILLFCNIYIYAAIRRQAAAVKQHKSKKTSNN---------------RNKQDGGGGGRRKPESVMKILAVLTVIHIITTLPASITQVL 316
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A267FSS9 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017324g3 PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.865e-15
Identity = 41/113 (36.28%), Postives = 62/113 (54.87%), Query Frame = 2 
Query:   26 RPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNK-------KRRTEHIVKVLTALSLIHAITTLPAAIYQAM 343
            R   D    ++F KLD +TY  +PY IL FCNIYIY  +++Q  A++ ++  K  N+               RNK       +R+ E ++K+L  L++IH ITTLPA+I Q +
Sbjct:  219 RCSYDKAFGQWFAKLDLVTYLFIPYTILLFCNIYIYAAIRRQAAAVKKHKSKKTSNN---------------RNKQDGGGGGRRKPESVMKILAVLTVIHIITTLPASITQVL 316
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A564YLH2 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS2349 PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 5.086e-13
Identity = 45/129 (34.88%), Postives = 73/129 (56.59%), Query Frame = 2 
Query:   59 FQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALR---NNQKLKI---------------------------KNSD--ASSGLESQIKP----IVKRN--KKRRTEHIVKVLTALSLIHAITTLPAAI 331
            F+K+DFI++A++PY+ILF CN+YIY +LK+Q + LR   N ++LK+                           KNS+   +S ++ Q+K     +  R+  +KR+ E ++KVLTAL+ +H + TLP  I
Sbjct:  187 FKKIDFISFAVIPYIILFSCNLYIYCKLKRQRRLLRTTMNQRQLKMNKSALLANSTSPATYTDAENPESLIVDKNSELMPNSAIDGQVKTPSECVSTRHERRKRKPEDVIKVLTALTAVHVVCTLPGTI 315
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042531.1 (SMESG000042531.1)

HSP 1 Score: 283.108 bits (723), Expect = 4.929e-97
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 2 
Query:    2 EKLALFECRPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQAMKSRIGVNKNQYMHTIIHENLLLL 412
            EKLALFECRPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQAMKSRIGVNKNQYMHTIIHENLLLL
Sbjct:  174 EKLALFECRPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQAMKSRIGVNKNQYMHTIIHENLLLL 310
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000042465.1 (SMESG000042465.1)

HSP 1 Score: 131.724 bits (330), Expect = 8.076e-38
Identity = 68/135 (50.37%), Postives = 96/135 (71.11%), Query Frame = 2 
Query:   11 ALFECRPQLDSGL-WKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQAMKSRIGVNKNQYMHTIIHENLLLL 412
             +  C+P+L+S + W FF K+D +TYA+LPY+ILFFCNIYIY +L++ H+ + N     I      + + ++  PI    KKRR EHI+KVLTALSLIHAIT+LPAAIYQ++K+++ +  N  MH +I E+LLLL
Sbjct:  178 TILACKPKLNSWISWGFF-KVDLLTYAVLPYLILFFCNIYIYIKLRK-HRIILNKTYQNIPKLQQMNLITNEETPIPVIPKKRRIEHIIKVLTALSLIHAITSLPAAIYQSLKAQLKIEDNPSMHAVIQESLLLL 310
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000054459.1 (SMESG000054459.1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.687e-19
Identity = 41/100 (41.00%), Postives = 66/100 (66.00%), Query Frame = 2 
Query:   50 WKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQAMKS 349
            +  F+K+DF+++A++PYVILF  NIYIY  L++QH  L        K+  +S+ L        K+ +KRR ++++K+LT L++IH ITTLP AI+  +K+
Sbjct:  175 FAIFKKIDFVSFAVIPYVILFSTNIYIYVVLRRQHILLS-------KHKSSSTLLND------KKRRKRRPQNVMKILTGLTIIHVITTLPGAIFTMLKN 261
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063789.1 (SMESG000063789.1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.340e-16
Identity = 35/95 (36.84%), Postives = 63/95 (66.32%), Query Frame = 2 
Query:   53 KFFQKLDFITYAILPYVILFFCNIYIYFRLKQQHKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLIHAITTLPAAIYQ 337
            + F K+DFITY++LPY ILF  N+YI+  L++Q + L+++   K  N+     +  +     K N+ R++++I+K+LTAL+++H IT++P  I+ 
Sbjct:  189 RVFSKIDFITYSVLPYFILFSTNMYIFIILRRQSRILKSHLNPKNTNNKNQRTVSRK-----KTNRGRQSQNIIKILTALTVVHMITSIPGTIFN 278
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3UHX36.495e-1734.90G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267EZ285.640e-1536.28G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267E7V25.865e-1536.28G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267FSS95.865e-1536.28G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A564YLH25.086e-1334.88G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000042531.14.929e-97100.00SMESG000042531.1[more]
SMESG000042465.18.076e-3850.37SMESG000042465.1[more]
SMESG000054459.12.687e-1941.00SMESG000054459.1[more]
SMESG000063789.11.340e-1636.84SMESG000063789.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014302 ID=SMED30014302|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=414bp
CGAAAAACTCGCTCTATTCGAATGCCGACCGCAATTAGACAGTGGGCTTT
GGAAATTTTTCCAGAAATTAGATTTTATTACTTACGCTATTTTACCTTAT
GTCATTCTGTTTTTTTGCAACATTTACATTTATTTTCGATTAAAACAACA
ACATAAAGCTCTGAGAAATAATCAAAAATTAAAAATCAAAAACTCCGATG
CTTCTAGTGGACTTGAGAGTCAAATAAAGCCGATTGTGAAACGAAATAAA
AAACGTCGCACCGAACACATTGTAAAGGTTCTTACGGCTCTGTCTTTAAT
CCACGCCATCACAACGCTGCCGGCGGCAATTTATCAGGCAATGAAGTCAC
GAATAGGTGTAAACAAAAACCAGTACATGCATACGATAATCCACGAGAAT
CTTTTACTTTTGAT
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protein sequence of SMED30014302-orf-1

>SMED30014302-orf-1 ID=SMED30014302-orf-1|Name=SMED30014302-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=137bp
EKLALFECRPQLDSGLWKFFQKLDFITYAILPYVILFFCNIYIYFRLKQQ
HKALRNNQKLKIKNSDASSGLESQIKPIVKRNKKRRTEHIVKVLTALSLI
HAITTLPAAIYQAMKSRIGVNKNQYMHTIIHENLLLL
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 2..136
e-value: 2.3E-12
score: 48.4
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 16..116
e-value: 6.98548E-5
score: 38.7681
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 13..115
NoneNo IPR availableTMHMMTMhelixcoord: 25..47
NoneNo IPR availableTMHMMTMhelixcoord: 90..112