Neutral ceramidase

Overview
NameNeutral ceramidase
Smed IDSMED30014265
Length (bp)2135
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Neutral ceramidase (SMED30014265) t-SNE clustered cells

Violin plots show distribution of expression levels for Neutral ceramidase (SMED30014265) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Neutral ceramidase (SMED30014265) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Neutral ceramidase (SMED30014265) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014265

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30014265SMESG000003228.1 Contig2188GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014265SMESG000003228.1 dd_Smed_v4_1726_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000051921.1 SMESG000051913.1 Contig977GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000003228.1 Contig11624newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000003228.1 Contig11624uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000051672.1 SMESG000051670.1 SMESG000003228.1 Contig22470newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000051672.1 SMESG000051670.1 SMESG000003228.1 Contig22470uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000003228.1 Contig39680newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000003228.1 Contig39680uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014265SMESG000003228.1 Contig977GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
goblet cellSMED30014265SMESG000003228.1 dd_Smed_v6_1726_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Neutral ceramidase vs. Ensembl Human
Match: ASAH2 (N-acylsphingosine amidohydrolase 2 [Source:HGNC Symbol;Acc:HGNC:18860])

HSP 1 Score: 640.958 bits (1652), Expect = 0.000e+0
Identity = 318/657 (48.40%), Postives = 418/657 (63.62%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRN--RPNLIYSFIFVDRF--VNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  A +N MGY    Q   GI  RLYSRAFI A P    R VF S+D GM+SQ ++ +V+  L+  +G+LY   NV LS TH+HSGP G+ QY +F I S GF N+T+  M TGI++SI  AH NMK G+IFIN G + G  INRSP SYL NP  ER RY  + DKEM VLK+V + G+++G+I+WFA+H  SMNN+N L++ DN GYAS + E+ KNK   PGQG FVAAFA +NLGDVSPNI G RCI++G  C+   STC  G    CIA GPG+DM +ST+IIG    Q A  LY +A  E+ G +   HQ+  MT++T+ L +    KTC P++GYSFAAGT DG G  +F QG    + FW+ IRD  +  PS E+  C  PKPILL TGE+S PH W P IV+ Q++ +GS+ I AIPGE TTMSGRR+R+A++         +N+TV+I+GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F NL +++  +    +  GP PPF + ++  L P I D +   R FG+VLQ             V FVGANP+N  +     +F+ V+++   + +W     DA WET+F W +        G S   ++W +P + QPG+YRIRY G
Sbjct:  102 YHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM-QNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKG-----LLGLSNATVEWHIPDTAQPGIYRIRYFG 752
[Close]
BLAST of Neutral ceramidase vs. Ensembl Human
Match: ASAH2 (N-acylsphingosine amidohydrolase 2 [Source:HGNC Symbol;Acc:HGNC:18860])

HSP 1 Score: 640.958 bits (1652), Expect = 0.000e+0
Identity = 318/657 (48.40%), Postives = 418/657 (63.62%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRN--RPNLIYSFIFVDRF--VNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  A +N MGY    Q   GI  RLYSRAFI A P    R VF S+D GM+SQ ++ +V+  L+  +G+LY   NV LS TH+HSGP G+ QY +F I S GF N+T+  M TGI++SI  AH NMK G+IFIN G + G  INRSP SYL NP  ER RY  + DKEM VLK+V + G+++G+I+WFA+H  SMNN+N L++ DN GYAS + E+ KNK   PGQG FVAAFA +NLGDVSPNI G RCI++G  C+   STC  G    CIA GPG+DM +ST+IIG    Q A  LY +A  E+ G +   HQ+  MT++T+ L +    KTC P++GYSFAAGT DG G  +F QG    + FW+ IRD  +  PS E+  C  PKPILL TGE+S PH W P IV+ Q++ +GS+ I AIPGE TTMSGRR+R+A++         +N+TV+I+GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F NL +++  +    +  GP PPF + ++  L P I D +   R FG+VLQ             V FVGANP+N  +     +F+ V+++   + +W     DA WET+F W +        G S   ++W +P + QPG+YRIRY G
Sbjct:  102 YHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM-QNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKG-----LLGLSNATVEWHIPDTAQPGIYRIRYFG 752
[Close]
BLAST of Neutral ceramidase vs. Ensembl Human
Match: ASAH2 (N-acylsphingosine amidohydrolase 2 [Source:HGNC Symbol;Acc:HGNC:18860])

HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0
Identity = 308/657 (46.88%), Postives = 402/657 (61.19%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRN--RPNLIYSFIFVDRF--VNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  A +N MGY    Q   GI  RLYSRAFI A P    R VF S+D GM+SQ ++ +V+  L+  +G+LY   NV LS TH+HSGP G+ QY +F I S GF N+T+  M TGI++SI  AH NMK G+IFIN G + G  INRSP SYL NP  ER RY  + DKEM VLK+V + G+++G+I+WFA+H  SMNN+N L++ DN GYAS + E+ KNK   PGQG FVAAFA +NLGDVSPNI G RCI++G  C+   STC  G    CIA GPG+DM +ST+IIG         +Y+ AK                             KTC P++GYSFAAGT DG G  +F QG    + FW+ IRD  +  PS E+  C  PKPILL TGE+S PH W P IV+ Q++ +GS+ I AIPGE TTMSGRR+R+A++         +N+TV+I+GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F NL +++  +    +  GP PPF + ++  L P I D +   R FG+VLQ             V FVGANP+N  +     +F+ V+++   + +W     DA WET+F W +        G S   ++W +P + QPG+YRIRY G
Sbjct:  102 YHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRA-------MYQRAK----------------------------SKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM-QNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKG-----LLGLSNATVEWHIPDTAQPGIYRIRYFG 717
[Close]
BLAST of Neutral ceramidase vs. Ensembl Human
Match: ASAH2 (N-acylsphingosine amidohydrolase 2 [Source:HGNC Symbol;Acc:HGNC:18860])

HSP 1 Score: 604.749 bits (1558), Expect = 0.000e+0
Identity = 308/657 (46.88%), Postives = 402/657 (61.19%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRN--RPNLIYSFIFVDRF--VNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  A +N MGY    Q   GI  RLYSRAFI A P    R VF S+D GM+SQ ++ +V+  L+  +G+LY   NV LS TH+HSGP G+ QY +F I S GF N+T+  M TGI++SI  AH NMK G+IFIN G + G  INRSP SYL NP  ER RY  + DKEM VLK+V + G+++G+I+WFA+H  SMNN+N L++ DN GYAS + E+ KNK   PGQG FVAAFA +NLGDVSPNI G RCI++G  C+   STC  G    CIA GPG+DM +ST+IIG         +Y+ AK                             KTC P++GYSFAAGT DG G  +F QG    + FW+ IRD  +  PS E+  C  PKPILL TGE+S PH W P IV+ Q++ +GS+ I AIPGE TTMSGRR+R+A++         +N+TV+I+GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F NL +++  +    +  GP PPF + ++  L P I D +   R FG+VLQ             V FVGANP+N  +     +F+ V+++   + +W     DA WET+F W +        G S   ++W +P + QPG+YRIRY G
Sbjct:   83 YHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRA-------MYQRAK----------------------------SKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM-QNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKG-----LLGLSNATVEWHIPDTAQPGIYRIRYFG 698
[Close]
BLAST of Neutral ceramidase vs. Ensembl Human
Match: ASAH2 (N-acylsphingosine amidohydrolase 2 [Source:HGNC Symbol;Acc:HGNC:18860])

HSP 1 Score: 572.392 bits (1474), Expect = 0.000e+0
Identity = 284/591 (48.05%), Postives = 379/591 (64.13%), Query Frame = 2 
Query:  254 KTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRN--RPNLIYSFIFVDRF--VNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            ++ V+  L+  +G+LY   NV LS TH+HSGP G+ QY +F I S GF N+T+  M TGI++SI  AH NMK G+IFIN G + G  INRSP SYL NP  ER RY  + DKEM VLK+V + G+++G+I+WFA+H  SMNN+N L++ DN GYAS + E+ KNK   PGQG FVAAFA +NLGDVSPNI G RCI++G  C+   STC  G    CIA GPG+DM +ST+IIG    Q A  LY +A  E+ G +   HQ+  MT++T+ L +    KTC P++GYSFAAGT DG G  +F QG    + FW+ IRD  +  PS E+  C  PKPILL TGE+S PH W P IV+ Q++ +GS+ I AIPGE TTMSGRR+R+A++         +N+TV+I+GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F NL +++  +    +  GP PPF + ++  L P I D +   R FG+VLQ             V FVGANP+N  +     +F+ V+++   + +W     DA WET+F W +        G S   ++W +P + QPG+YRIRY G
Sbjct:   10 RSDVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM-QNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKG-----LLGLSNATVEWHIPDTAQPGIYRIRYFG 594
[Close]
BLAST of Neutral ceramidase vs. Ensembl Fly
Match: CDase (gene:FBgn0039774 transcript:FBtr0331357)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 325/696 (46.70%), Postives = 435/696 (62.50%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
             +A++ L  +VS  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GN+Y   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRI ++   +L  NINRSP+SYL NP+EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C      C A GPGKDM EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI  P+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT++M++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  ++R +N+  W   +TDA WETK  W + N I    G SE+ I WD+     PG YRIR+ G  K +        G+T+SF V
Sbjct:   11 FLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGN-RVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVG-GIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTI---LGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. Ensembl Fly
Match: CDase (gene:FBgn0039774 transcript:FBtr0085648)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 325/696 (46.70%), Postives = 435/696 (62.50%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
             +A++ L  +VS  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GN+Y   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRI ++   +L  NINRSP+SYL NP+EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C      C A GPGKDM EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI  P+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT++M++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  ++R +N+  W   +TDA WETK  W + N I    G SE+ I WD+     PG YRIR+ G  K +        G+T+SF V
Sbjct:   11 FLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGN-RVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVG-GIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTI---LGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. Ensembl Fly
Match: CDase (gene:FBgn0039774 transcript:FBtr0085645)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 325/696 (46.70%), Postives = 435/696 (62.50%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
             +A++ L  +VS  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GN+Y   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRI ++   +L  NINRSP+SYL NP+EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C      C A GPGKDM EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI  P+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT++M++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  ++R +N+  W   +TDA WETK  W + N I    G SE+ I WD+     PG YRIR+ G  K +        G+T+SF V
Sbjct:   11 FLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGN-RVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVG-GIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTI---LGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. Ensembl Fly
Match: CDase (gene:FBgn0039774 transcript:FBtr0085646)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 325/696 (46.70%), Postives = 435/696 (62.50%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
             +A++ L  +VS  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GN+Y   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRI ++   +L  NINRSP+SYL NP+EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C      C A GPGKDM EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI  P+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT++M++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  ++R +N+  W   +TDA WETK  W + N I    G SE+ I WD+     PG YRIR+ G  K +        G+T+SF V
Sbjct:   11 FLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGN-RVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVG-GIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTI---LGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. Ensembl Fly
Match: CDase (gene:FBgn0039774 transcript:FBtr0085647)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 325/696 (46.70%), Postives = 435/696 (62.50%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
             +A++ L  +VS  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GN+Y   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRI ++   +L  NINRSP+SYL NP+EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C      C A GPGKDM EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI  P+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT++M++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  ++R +N+  W   +TDA WETK  W + N I    G SE+ I WD+     PG YRIR+ G  K +        G+T+SF V
Sbjct:   11 FLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGN-RVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVG-GIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTI---LGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. Ensembl Zebrafish
Match: asah2 (N-acylsphingosine amidohydrolase 2 [Source:NCBI gene;Acc:493602])

HSP 1 Score: 594.349 bits (1531), Expect = 0.000e+0
Identity = 301/662 (45.47%), Postives = 423/662 (63.90%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQ-GKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPP-FNED-VVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNL-IYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK 2011
            YLIG G +D TGP A +  MGYAN  Q   G+H RL+SRAFI        R VF + D GM+SQ ++ +V + LK+ +G+LY + NV LS TH+HSG GG+ QY LF ITS G++  +  A+ +GIV+SI  AH N++ GRIFIN G++  +N NRSP SY+ NP EER RY+++ DK++ VLK   ++G+ IG+++WFAVH  SMN TN+++S DN GYAS +FE+ KN    PG+ G FVA F+ +NLGD SPNI G  C+++G  C+   S+C  G  + CIAFGPG+DM EST+IIGE   + A  LY +AK E+ G +   HQ+  MT+ T++  +   G+TC P++G+SFAAGTTDG G F+F QG    + FW+ IRD  +  PS E   C  PKPIL +TGE+  P  W P IV+ Q++ IGS+ + A+PGE TTMSGRR+R+A+K+ ++ +    N  V++AGL N Y+ YI T+EEYQ QRYE ASTI+GPHTL A++ ++  L +++       +P GP PP F+ED + + +   + D +     FG+VLQ+           SV FV  NPR+  ++   + + V+RF N T  W     DA WET+F W     I    G S+  ++W +P + Q G Y+I+Y G  K
Sbjct:   61 YLIGVGRADCTGPVADLPMMGYANTDQTARGLHTRLFSRAFIVDD--GNKRVVFVTSDIGMVSQRLRLEVFQALKEKYGDLYRQDNVVLSGTHTHSGVGGYFQYTLFMITSKGYIKPSIQAIVSGIVKSIDIAHRNLRPGRIFINKGQVADSNFNRSPHSYMNNPEEERNRYEFNTDKQIVVLKFTDLDGDGIGLLSWFAVHPVSMNYTNRMVSSDNLGYASYIFEQEKNIGFLPGEKGPFVAGFSSSNLGDSSPNIRGPVCVNTGLKCDYINSSCPVGGKKACIAFGPGEDMFESTRIIGENMFKIAKELYGSAKQELHGPVYGAHQWVNMTDETVQFNSTHTGRTCKPALGHSFAAGTTDGGGEFNFLQGDTEGDPFWDGIRDAVLGPPSNETKACHQPKPILFSTGEMDSPLPWHPAIVDVQIITIGSLAVVAVPGEFTTMSGRRIREAVKRELEVKEPFTNAEVVVAGLCNIYTHYITTYEEYQIQRYEGASTIFGPHTLSAYIQRYRGLAKAIAHGTIGELPKGPEPPFFDEDKLFNQVRDPVADVAPVGTTFGDVLQEVNPVYKVGEIASVTFVSGNPRHSGDIRDTTLVTVERFHNDTGSWEIIHNDASWETRFHW-----IKGLAGRSQAKVEWHIPQTAQAGTYQIQYFGHYK 715
[Close]
BLAST of Neutral ceramidase vs. Ensembl Xenopus
Match: asah2 (N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [Source:NCBI gene;Acc:100170585])

HSP 1 Score: 621.698 bits (1602), Expect = 0.000e+0
Identity = 319/681 (46.84%), Postives = 433/681 (63.58%), Query Frame = 2 
Query:   59 LIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIR-TPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPP-FNE-DVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIY--SFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK--SLFSKYQGIHGVTNSFNV 2065
            LIG G +D TG  A V  MGYA+  Q+  GIH R YSRAFI A P N  R V+ S D GM+SQ V+ +V+++LK  +GNLY ++NV LS TH+HSGP G+ QY  F +T  GF+    +A+  GIV+SI  AH NMK+GRIF+N G +  + +NRSP+SYL NP  ER RY  + DK+M VLK+V M  +++G+I+WFAVH  SM N N LIS DN GYAS +FE+ KN    PG+G FVAAF  +N GDVSPN  G  CI++G+ C+   STC  G    C+A GPG DM E+TKIIGE   Q A  L+ N+  E++  + + HQ+  M+N+T++L       TC P++GYSFAAGT DGPG  +F QG V  + FW+ IRD I   PS E   C +PKPIL+ TGE+S P  W P IV+ Q+  IGS+ + A+PGE TTMSGRR+R+ ++KV+      ++V V+++GL N Y+ YI TFEEYQ QRYEAASTIYGPHTL A+   F  L R++V+N+   +P GP PP FNE  + SFL PV  D +  N  FG+VL++     +     SV FV  NPR+    +   +++ V+++   T  W T   DA WET++ W + +       +S   ++W +P S QPG+YRI + G  K  +L    +   G+++ F V
Sbjct:   86 LIGVGRADCTGLLADVPLMGYASSDQLAGGIHTRQYSRAFIIAEPDNSKRLVYVSADIGMVSQRVRLEVMEKLKAKYGNLYRQENVILSGTHTHSGPAGYFQYTTFMLTGKGFIRPATDAIVNGIVKSIDQAHQNMKRGRIFLNKGTVENSQLNRSPSSYLQNPESERLRYPSNTDKDMVVLKMVDMHNQDMGIISWFAVHAVSMPNVNHLISSDNLGYASYLFEQEKNSGYLPGEGPFVAAFTTSNHGDVSPNTKGPWCINTGDSCDNPGSTCPIGGVSTCVALGPGNDMFENTKIIGEHIFQKAKELFSNSSRELEKRVDFAHQWVDMSNVTVQLNGTHSATTCKPALGYSFAAGTIDGPGLINFTQGAVEGDPFWDGIRDAILGVPSNETQECHSPKPILINTGEMSRPLPWHPTIVDVQMFTIGSLAMLAVPGEFTTMSGRRLREDVQKVLDPAGT-QDVQVVLSGLCNVYTHYITTFEEYQAQRYEAASTIYGPHTLAAYQQLFRGLARAIVENKVAELPKGPQPPFFNESQLFSFLLPVPVDKAPGNTTFGDVLEEVKPQYTVGEVASVTFVTGNPRSSVEHLAGKTYLAVEKYNKDTGEWHTVHNDASWETRYYWNKVS-----RDQSNGTMEWHIPPSAQPGIYRIHHFGHYKERNLPVTIKPYEGISSEFEV 760
[Close]
BLAST of Neutral ceramidase vs. Ensembl Xenopus
Match: asah2 (N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [Source:NCBI gene;Acc:100170585])

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 317/679 (46.69%), Postives = 430/679 (63.33%), Query Frame = 2 
Query:   59 LIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIR-TPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPP-FNE-DVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK--SLFSKYQGIHGVTNSFNV 2065
            LIG G +D TG  A V  MGYA+  Q+  GIH R YSRAFI A P N  R V+ S D GM+SQ V+ +V+++LK  +GNLY ++NV LS TH+HSGP G+ QY  F +T  GF+    +A+  GIV+SI  AH NMK+GRIF+N G +  + +NRSP+SYL NP  ER RY  + DK+M VLK+V M  +++G+I+WFAVH  SM N N LIS DN GYAS +FE+ KN    PG+G FVAAF  +N GDVSPN  G  CI++G+ C+   STC  G    C+A GPG DM E+TKIIGE   Q A  L+ N+  E++  + + HQ+  M+N+T++L       TC P++GYSFAAGT DGPG  +F QG V  + FW+ IRD I   PS E   C +PKPIL+ TGE+S P  W P IV+ Q+  IGS+ + A+PGE TTMSGRR+R+ ++KV+      ++V V+++GL N Y+ YI TFEEYQ QRYEAASTIYGPHTL A+   F  L R++V+N+   +P GP PP FNE  + SFL PV  D +  N  FG+VL++    V    TVS+  + A          +++ V+++   T  W T   DA WET++ W + +       +S   ++W +P S QPG+YRI + G  K  +L    +   G+++ F V
Sbjct:   86 LIGVGRADCTGLLADVPLMGYASSDQLAGGIHTRQYSRAFIIAEPDNSKRLVYVSADIGMVSQRVRLEVMEKLKAKYGNLYRQENVILSGTHTHSGPAGYFQYTTFMLTGKGFIRPATDAIVNGIVKSIDQAHQNMKRGRIFLNKGTVENSQLNRSPSSYLQNPESERLRYPSNTDKDMVVLKMVDMHNQDMGIISWFAVHAVSMPNVNHLISSDNLGYASYLFEQEKNSGYLPGEGPFVAAFTTSNHGDVSPNTKGPWCINTGDSCDNPGSTCPIGGVSTCVALGPGNDMFENTKIIGEHIFQKAKELFSNSSRELEKRVDFAHQWVDMSNVTVQLNGTHSATTCKPALGYSFAAGTIDGPGLINFTQGAVEGDPFWDGIRDAILGVPSNETQECHSPKPILINTGEMSRPLPWHPTIVDVQMFTIGSLAMLAVPGEFTTMSGRRLREDVQKVLDPAGT-QDVQVVLSGLCNVYTHYITTFEEYQAQRYEAASTIYGPHTLAAYQQLFRGLARAIVENKVAELPKGPQPPFFNESQLFSFLLPVPVDKAPGNTTFGDVLEE----VKPQYTVSLFVLQAGK--------TYLAVEKYNKDTGEWHTVHNDASWETRYYWNKVS-----RDQSNGTMEWHIPPSAQPGIYRIHHFGHYKERNLPVTIKPYEGISSEFEV 746
[Close]
BLAST of Neutral ceramidase vs. Ensembl Xenopus
Match: asah2 (N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [Source:NCBI gene;Acc:100170585])

HSP 1 Score: 600.127 bits (1546), Expect = 0.000e+0
Identity = 316/679 (46.54%), Postives = 426/679 (62.74%), Query Frame = 2 
Query:   59 LIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIR-TPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPP-FNE-DVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK--SLFSKYQGIHGVTNSFNV 2065
            LIG G +D TG  A V  MGYA+  Q+  GIH R YSRAFI A P N  R V+ S D GM+SQ V+ +V+++LK  +GNLY ++NV LS TH+HSGP G+ QY  F +T  GF+    +A+  GIV+SI  AH NMK+GRIF+N G +  + +NRSP+SYL NP  ER RY  + DK+M VLK+V M  +++G+I+WFAVH  SM N N LIS DN GYAS +FE+ KN    PG+G FVAAF  +N GDVSPN  G  CI++G+ C+   STC  G    C+A GPG DM E+TKIIGE   Q A  L+ N+  E++  + + HQ+  M+N+T++L       TC P++GYSFAAGT DGPG  +F QG V  + FW+ IRD I   PS E   C +PKPIL+ TGE+S P  W P IV+ Q+  IGS+ + A+PGE TTMSGRR+R+ +     +    ++V V+++GL N Y+ YI TFEEYQ QRYEAASTIYGPHTL A+   F  L R++V+N+   +P GP PP FNE  + SFL PV  D +  N  FG+VL++    V    TVSV               +++ V+++   T  W T   DA WET++ W   N ++    +S   ++W +P S QPG+YRI + G  K  +L    +   G+++ F V
Sbjct:   59 LIGVGRADCTGLLADVPLMGYASSDQLAGGIHTRQYSRAFIIAEPDNSKRLVYVSADIGMVSQRVRLEVMEKLKAKYGNLYRQENVILSGTHTHSGPAGYFQYTTFMLTGKGFIRPATDAIVNGIVKSIDQAHQNMKRGRIFLNKGTVENSQLNRSPSSYLQNPESERLRYPSNTDKDMVVLKMVDMHNQDMGIISWFAVHAVSMPNVNHLISSDNLGYASYLFEQEKNSGYLPGEGPFVAAFTTSNHGDVSPNTKGPWCINTGDSCDNPGSTCPIGGVSTCVALGPGNDMFENTKIIGEHIFQKAKELFSNSSRELEKRVDFAHQWVDMSNVTVQLNGTHSATTCKPALGYSFAAGTIDGPGLINFTQGAVEGDPFWDGIRDAILGVPSNETQECHSPKPILINTGEMSRPLPWHPTIVDVQMFTIGSLAMLAVPGEFTTMSGRRLREDV----LDPAGTQDVQVVLSGLCNVYTHYITTFEEYQAQRYEAASTIYGPHTLAAYQQLFRGLARAIVENKVAELPKGPQPPFFNESQLFSFLLPVPVDKAPGNTTFGDVLEE----VKPQYTVSVWHQKQKSLFVLQAGKTYLAVEKYNKDTGEWHTVHNDASWETRYYW---NKVS--RDQSNGTMEWHIPPSAQPGIYRIHHFGHYKERNLPVTIKPYEGISSEFEV 724
[Close]
BLAST of Neutral ceramidase vs. Ensembl Xenopus
Match: grin2c (glutamate receptor, ionotropic, N-methyl D-aspartate 2C [Source:Xenbase;Acc:XB-GENE-993386])

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 302/649 (46.53%), Postives = 407/649 (62.71%), Query Frame = 2 
Query:  113 MGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPF-NEDVV-SFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQTWSTEFTDADWETKFQWFQKNIIA---FFFG----------ESEVVIDWDVPGSQQPGLYRIRYEG 2002
            MGYA   QI  G+H+R YSRAFI A P +  R V+ S + GM+SQ V+ +V++ELK  +GNLY E NV LS++++HSGP G+ QY  F + S GF     N + +GIV+SI  AH NMK+G+IF+N G +  + INRSP+SYL NP  ER RY  + DK+M +LK+V M  +++GMI+WFAVHG SM N NKLIS DN GYAS +FE+ KN  S PG G FVAAF+ +NLGDVSPN  G  C+++   C+  TSTC  G +  C+A GPGKDM ESTKIIGER       LY NA  E+   + + HQ+  M+N+T+KL      KTC P++GYSFAAGTTDG G+ +F QGT+  + +WN IRD ++  PS E   C  PKPIL+ TG++  P  W P IV+ Q+  IGS+ + A+PGE TTM+GRR+++ +KK++ + +   N  V+++GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A+L  F+ L +++V+N T  +P GP PP  NE ++ + LPPV  D +  N+ FG+VL+       R   VS  FV ANPR+  N + +                         +W+   II    F FG          +S    +W +P S +PGLY I   G
Sbjct:   30 MGYAKSDQIASGLHLRQYSRAFIIAEPDDSKRVVYVSTNIGMMSQRVRLEVMEELKVNYGNLYREDNVILSASNTHSGPAGYFQYNTFVLASQGFNRPQTNVIVSGIVKSIERAHQNMKRGKIFMNKGTVENSQINRSPSSYLLNPESERIRYSSNTDKDMVILKMVDMNDQDMGMISWFAVHGVSMGNANKLISSDNLGYASYLFEQEKNNRSLPGTGSFVAAFSPSNLGDVSPNTMGPHCVNANETCDNPTSTCPAGGSVACVALGPGKDMFESTKIIGERIFLK--ELYGNASRELDKKVNFAHQWVDMSNVTVKLNETHSVKTCKPALGYSFAAGTTDGAGSINFTQGTLEGDLYWNAIRDLYLGAPSKETQECHHPKPILINTGQMDKPIPWHPKIVDIQMFTIGSIALLALPGEFTTMAGRRLQEEVKKIL-DPSGTLNTDVVLSGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYLQLFNGLAKAIVENTTDELPKGPRPPLINESLLPNSLPPVPPDSTPANKDFGDVLKDVYPRYRRGDIVSCTFVAANPRSGVNRMPTVCIYS--------------------EWWHNCIITTVIFLFGRYYWHKGPGNQSNATAEWHIPLSAEPGLYYIEVCG 655
[Close]
BLAST of Neutral ceramidase vs. Ensembl Xenopus
Match: asah2 (N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 [Source:NCBI gene;Acc:100170585])

HSP 1 Score: 564.303 bits (1453), Expect = 0.000e+0
Identity = 303/701 (43.22%), Postives = 425/701 (60.63%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNF------MGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIR-TPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET-IPVGPTPPFNEDVVSFLPPVIF-DYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIY---SFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK--SLFSKYQGIHGVTNSFNV 2065
            LI L +L   VS   +    I     P   ++F      MGYA+  Q+  GIH R YSRAFI A P N  R V+ S D GM+SQ V+ +V+++LK  +GNLY ++NV LS TH+HSGP G+ QY  F +T  GF+    +A+  GIV+SI  AH NMK+GRIF+N G +  + +NRSP+SYL NP  ER RY  + DK+M VLK+V M  +++G+I+WFAVH  SM N N LIS DN GYAS +FE+ KN    PG+G FVAAF  +N GDVSPN  G  CI++G+ C+   STC  G    C+A GPG DM E+TKIIGE   Q A  L+ N+  E++  + + HQ+  M+N+T++L       TC P++GYSFAAGT DGPG  +F QG V  + FW+ IRD I   PS E   C +PKPIL+ TGE+S P  W P IV+ Q+  IGS+ + A+PGE TTMSGRR+R+ ++KV+      ++V V+++GL N Y+ YI TFEEYQ QRYEAASTIYGPHTL A+   F  L R++V+  T +  G +P       S L  + +  ++   + + E++      +  +S +   F G N R    ++    +++ V+++   T  W T   DA WET++ W + +       +S   ++W +P S QPG+YRI + G  K  +L    +   G+++ F V
Sbjct:   16 LIVLFVLSVCVSAGLIAALFILFQIYPCHALHFCAFIPQMGYASSDQLAGGIHTRQYSRAFIIAEPDNSKRLVYVSADIGMVSQRVRLEVMEKLKAKYGNLYRQENVILSGTHTHSGPAGYFQYTTFMLTGKGFIRPATDAIVNGIVKSIDQAHQNMKRGRIFLNKGTVENSQLNRSPSSYLQNPESERLRYPSNTDKDMVVLKMVDMHNQDMGIISWFAVHAVSMPNVNHLISSDNLGYASYLFEQEKNSGYLPGEGPFVAAFTTSNHGDVSPNTKGPWCINTGDSCDNPGSTCPIGGVSTCVALGPGNDMFENTKIIGEHIFQKAKELFSNSSRELEKRVDFAHQWVDMSNVTVQLNGTHSATTCKPALGYSFAAGTIDGPGLINFTQGAVEGDPFWDGIRDAILGVPSNETQECHSPKPILINTGEMSRPLPWHPTIVDVQMFTIGSLAMLAVPGEFTTMSGRRLREDVQKVLDPAGT-QDVQVVLSGLCNVYTHYITTFEEYQAQRYEAASTIYGPHTLAAYQQLFRGLARAIVEVSTAVLYGGSP-----AGSLLCHISWITWAPLGQSYREIICAKQRALHSHSRMLCTFSG-NCRGSLFVLQAGKTYLAVEKYNKDTGEWHTVHNDASWETRYYWNKVS-----RDQSNGTMEWHIPPSAQPGIYRIHHFGHYKERNLPVTIKPYEGISSEFEV 704
[Close]
BLAST of Neutral ceramidase vs. Ensembl Mouse
Match: Asah2 (N-acylsphingosine amidohydrolase 2 [Source:MGI Symbol;Acc:MGI:1859310])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 313/657 (47.64%), Postives = 421/657 (64.08%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRP--NLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  + +N MGY    Q   G+  RL+SRAFI A P    R  F SV+  MISQ ++ +V+K L+  +G+LY   NV LS+ H+HSGP GF QY L+ + S GF N T+  + +GI++SI  AH N+K G+IFIN G +    INRSP+SYL NP  ER RY  + DKEM VLKLV + GE++G+I+WFA+H  SMNN+N  ++ DN GYA+ +FE+ KNK   PGQG FVA FA +NLGDVSPNI G  C+++G  C+   STC +G    C+A GPG+DM EST IIG    Q A  LY +A  E+ G +   HQ+  MT+++++L      KTC P++GYSFAAGT DG    +  QGT   + FW+ +RD  +  PS E+V CQ PKPILL +GE++ PH WQP IV+ Q++ +GS+ IAAIPGELTTMSGRR R+AIKK        +++TV+IAGLSN Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F +L +++  +    +  GP PPF +++++ L P I D +   + FG+VLQ           V V FVGANP+N        +F+ V+++ +    W   + DA WET+F W  K I+    G S   I W +P +  PG+YRIRY G
Sbjct:   78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGM-KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW-HKGIL----GLSNATIYWHIPDTAYPGIYRIRYFG 728
[Close]
BLAST of Neutral ceramidase vs. Ensembl Mouse
Match: Asah2 (N-acylsphingosine amidohydrolase 2 [Source:MGI Symbol;Acc:MGI:1859310])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 313/657 (47.64%), Postives = 421/657 (64.08%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRP--NLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  + +N MGY    Q   G+  RL+SRAFI A P    R  F SV+  MISQ ++ +V+K L+  +G+LY   NV LS+ H+HSGP GF QY L+ + S GF N T+  + +GI++SI  AH N+K G+IFIN G +    INRSP+SYL NP  ER RY  + DKEM VLKLV + GE++G+I+WFA+H  SMNN+N  ++ DN GYA+ +FE+ KNK   PGQG FVA FA +NLGDVSPNI G  C+++G  C+   STC +G    C+A GPG+DM EST IIG    Q A  LY +A  E+ G +   HQ+  MT+++++L      KTC P++GYSFAAGT DG    +  QGT   + FW+ +RD  +  PS E+V CQ PKPILL +GE++ PH WQP IV+ Q++ +GS+ IAAIPGELTTMSGRR R+AIKK        +++TV+IAGLSN Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F +L +++  +    +  GP PPF +++++ L P I D +   + FG+VLQ           V V FVGANP+N        +F+ V+++ +    W   + DA WET+F W  K I+    G S   I W +P +  PG+YRIRY G
Sbjct:   78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGM-KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW-HKGIL----GLSNATIYWHIPDTAYPGIYRIRYFG 728
[Close]
BLAST of Neutral ceramidase vs. Ensembl Mouse
Match: Asah2 (N-acylsphingosine amidohydrolase 2 [Source:MGI Symbol;Acc:MGI:1859310])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 313/657 (47.64%), Postives = 421/657 (64.08%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRP--NLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  + +N MGY    Q   G+  RL+SRAFI A P    R  F SV+  MISQ ++ +V+K L+  +G+LY   NV LS+ H+HSGP GF QY L+ + S GF N T+  + +GI++SI  AH N+K G+IFIN G +    INRSP+SYL NP  ER RY  + DKEM VLKLV + GE++G+I+WFA+H  SMNN+N  ++ DN GYA+ +FE+ KNK   PGQG FVA FA +NLGDVSPNI G  C+++G  C+   STC +G    C+A GPG+DM EST IIG    Q A  LY +A  E+ G +   HQ+  MT+++++L      KTC P++GYSFAAGT DG    +  QGT   + FW+ +RD  +  PS E+V CQ PKPILL +GE++ PH WQP IV+ Q++ +GS+ IAAIPGELTTMSGRR R+AIKK        +++TV+IAGLSN Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F +L +++  +    +  GP PPF +++++ L P I D +   + FG+VLQ           V V FVGANP+N        +F+ V+++ +    W   + DA WET+F W  K I+    G S   I W +P +  PG+YRIRY G
Sbjct:   78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGM-KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW-HKGIL----GLSNATIYWHIPDTAYPGIYRIRYFG 728
[Close]
BLAST of Neutral ceramidase vs. Ensembl Mouse
Match: Asah2 (N-acylsphingosine amidohydrolase 2 [Source:MGI Symbol;Acc:MGI:1859310])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 313/657 (47.64%), Postives = 421/657 (64.08%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRP--NLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  + +N MGY    Q   G+  RL+SRAFI A P    R  F SV+  MISQ ++ +V+K L+  +G+LY   NV LS+ H+HSGP GF QY L+ + S GF N T+  + +GI++SI  AH N+K G+IFIN G +    INRSP+SYL NP  ER RY  + DKEM VLKLV + GE++G+I+WFA+H  SMNN+N  ++ DN GYA+ +FE+ KNK   PGQG FVA FA +NLGDVSPNI G  C+++G  C+   STC +G    C+A GPG+DM EST IIG    Q A  LY +A  E+ G +   HQ+  MT+++++L      KTC P++GYSFAAGT DG    +  QGT   + FW+ +RD  +  PS E+V CQ PKPILL +GE++ PH WQP IV+ Q++ +GS+ IAAIPGELTTMSGRR R+AIKK        +++TV+IAGLSN Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F +L +++  +    +  GP PPF +++++ L P I D +   + FG+VLQ           V V FVGANP+N        +F+ V+++ +    W   + DA WET+F W  K I+    G S   I W +P +  PG+YRIRY G
Sbjct:   78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGM-KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW-HKGIL----GLSNATIYWHIPDTAYPGIYRIRYFG 728
[Close]
BLAST of Neutral ceramidase vs. Ensembl Mouse
Match: Asah2 (N-acylsphingosine amidohydrolase 2 [Source:MGI Symbol;Acc:MGI:1859310])

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 313/657 (47.64%), Postives = 421/657 (64.08%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRP--NLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  + +N MGY    Q   G+  RL+SRAFI A P    R  F SV+  MISQ ++ +V+K L+  +G+LY   NV LS+ H+HSGP GF QY L+ + S GF N T+  + +GI++SI  AH N+K G+IFIN G +    INRSP+SYL NP  ER RY  + DKEM VLKLV + GE++G+I+WFA+H  SMNN+N  ++ DN GYA+ +FE+ KNK   PGQG FVA FA +NLGDVSPNI G  C+++G  C+   STC +G    C+A GPG+DM EST IIG    Q A  LY +A  E+ G +   HQ+  MT+++++L      KTC P++GYSFAAGT DG    +  QGT   + FW+ +RD  +  PS E+V CQ PKPILL +GE++ PH WQP IV+ Q++ +GS+ IAAIPGELTTMSGRR R+AIKK        +++TV+IAGLSN Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F +L +++  +    +  GP PPF +++++ L P I D +   + FG+VLQ           V V FVGANP+N        +F+ V+++ +    W   + DA WET+F W  K I+    G S   I W +P +  PG+YRIRY G
Sbjct:   78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKA-KLYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGM-KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW-HKGIL----GLSNATIYWHIPDTAYPGIYRIRYFG 727
[Close]
BLAST of Neutral ceramidase vs. UniProt/SwissProt
Match: sp|Q29C43|NCASE_DROPS (Neutral ceramidase OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=CDase PE=3 SV=1)

HSP 1 Score: 656.366 bits (1692), Expect = 0.000e+0
Identity = 327/696 (46.98%), Postives = 439/696 (63.07%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
            LIAL+ L  + S  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GNLY   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRIF++   +L  NINRSPTSYL NP EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C     +C A GPG+DM+EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI TP+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT+++++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  V+R +N+  W   +TDA WETK  W + N I    G S++ I W++     PG+YRIR+ G  K +        G+++SF V
Sbjct:   11 LIALSGLCGLASATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKG-NRVAFVSADAGMMGYGLKREVIKRLQARYGNLYHTDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRIFLSKTTVLNVNINRSPTSYLRNPEEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPAGEGECFASGPGRDMVESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSAYNPLSRKIDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIATPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAAG-GLDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKALMRNETVEPGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKTQPEKEYGINDTVKVTYISGNPRNNLFTEKTYFTVERKINEDRWKVAYTDASWETKMIWHRTNTI---LGFSDLEIYWNISPQTLPGVYRIRHSGEYKYILGGKYPYEGLSHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. UniProt/SwissProt
Match: sp|Q9VA70|NCASE_DROME (Neutral ceramidase OS=Drosophila melanogaster OX=7227 GN=CDase PE=1 SV=1)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 325/696 (46.70%), Postives = 435/696 (62.50%), Query Frame = 2 
Query:   14 LIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLY-----ENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKT-----CLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDV-VSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT-WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
             +A++ L  +VS  Y +G G +DITGP  ++NFMGYAN  Q+G GIH R+++RAF+        R  F S D GM+   +K +V+K L+  +GN+Y   NVA+S TH+H  PGGF  ++L+DI+ LGFV +T+  M  G+   I  A +N+  GRI ++   +L  NINRSP+SYL NP+EER +Y++D DK +T L+ V +E   +G  NW+AVH TSMNNTN+L++ DN GYA+++ E+  N N  PG+GKFV AF  +NLGDVSPNI G +C  SGN C+  TS C      C A GPGKDM EST+I+G+R   AAL L      E+   E+ G +++ HQF  M N      N    K      C P+MGYSFAAGTTDGPGAF F+QGT T N  WNF+RDFI  P+ E + C  PKPILLATG  +FP+ WQP IV  Q+++IG V+IAA+P E TTM+GRR+R+ I+        G +  VIIAGL+N Y+ Y  T EEYQ QRYEAASTI+GPHT   ++  F  LT++M++NET+  GP+PP+  DV +S    V+FD    N  FG V  QP      N TV V ++  NPRN      ++  ++R +N+  W   +TDA WETK  W + N I    G SE+ I WD+     PG YRIR+ G  K +        G+T+SF V
Sbjct:   11 FLAVSFLCGLVSATYKVGVGRADITGPPVEINFMGYANIKQVGRGIHTRVFARAFVVEDEKGN-RVAFVSADAGMMGYGLKREVIKRLQARYGNIYHNDNVAISGTHTHGAPGGFLMHLLYDISILGFVPQTFEVMAQGLYLCIKRATDNLVDGRILLSKTTVLNVNINRSPSSYLRNPAEERAQYEHDTDKTLTQLRFVDLENNLLGAFNWYAVHATSMNNTNRLVTSDNVGYAALLLEKEYNPNKMPGKGKFVGAFCSSNLGDVSPNIMGPKCSISGNECDLLTSRCPTGEGDCFASGPGKDMFESTQILGQRLADAALGLLNEQSQESTAREVTGDVRFIHQFVDMPNYNGSTYNPLSRKVDKIRGCQPAMGYSFAAGTTDGPGAFSFEQGTTTDNPMWNFVRDFIAAPTQEDIKCHEPKPILLATGRATFPYEWQPKIVSDQLLKIGDVIIAAVPCEFTTMAGRRLRNQIRAAASAVG-GIDTEVIIAGLTNIYTSYTVTPEEYQAQRYEAASTIFGPHTHSIYMDVFERLTKAMMRNETVDAGPSPPYMNDVMLSLNTGVLFDGHPINTDFGYVKSQPNKEYGINETVKVTYISGNPRNNLFTEKTYFTIERKINEDRWKVAYTDASWETKMVWHRTNTI---LGFSEMDIYWDISPQTLPGEYRIRHSGEYKYILGGKYPYEGLTHSFTV 701
[Close]
BLAST of Neutral ceramidase vs. UniProt/SwissProt
Match: sp|Q55G11|NCSEB_DICDI (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 643.654 bits (1659), Expect = 0.000e+0
Identity = 341/676 (50.44%), Postives = 439/676 (64.94%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFG-NLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGK--DMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSW---GKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTT--NSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPP-FNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFV-NQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK-----SLFSKYQGI 2038
            Y IGAGI DITG +A+VN MGYANP Q+G GIH R  +RAF+F   SN  R V+ S D  MI Q VK  VV+ L+ IFG N+Y+E NV LS TH+HSGP GF QY L+ ITSLGF  + ++ +  GIV++IV AH +++   +F  +GEL   NINRSP +Y  NP EE+  Y  +VDK MTVL++  M G     I++FAVH TSMNNTN LISGDNKGYAS ++E+  N     G+G FVAAF Q+N GDVSPN  G  C D G+PC+  TSTC+GRNE+C + GPGK  DM EST+IIG  Q   AL L+ NA I++ G +QY+H + Q TN++++   +S      TC  +MGYSFAAGTTDGPGAF+F Q    T  N FWNFI DFI  P+P+ + CQ+PKPILL  G +  P  W P ++  Q++ IG +V+ A+PGE TTMSGRR+R+++++++ E    EN  V+IAGLSNTYS YIATFEEYQ QRYE AST++GPHTL +++ +F  L +S+V   T+P GPTP       + FLPPVI D +     FGEV      N S N TVS  F G NPRN   +  SF+ VD       W+T   D DW+TKF+W   ++     G S + I+W +     PG YRI + G AK     S  + YQGI
Sbjct:   46 YQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVD-SNGNRAVYVSTDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLRIEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWEKQVNGPGTAGKGPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVGMVE-PIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREIIGESI--ENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNLNYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDL-----GFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGI 711
[Close]
BLAST of Neutral ceramidase vs. UniProt/SwissProt
Match: sp|Q9NR71|ASAH2_HUMAN (Neutral ceramidase OS=Homo sapiens OX=9606 GN=ASAH2 PE=1 SV=2)

HSP 1 Score: 640.958 bits (1652), Expect = 0.000e+0
Identity = 318/657 (48.40%), Postives = 418/657 (63.62%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRN--RPNLIYSFIFVDRF--VNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  A +N MGY    Q   GI  RLYSRAFI A P    R VF S+D GM+SQ ++ +V+  L+  +G+LY   NV LS TH+HSGP G+ QY +F I S GF N+T+  M TGI++SI  AH NMK G+IFIN G + G  INRSP SYL NP  ER RY  + DKEM VLK+V + G+++G+I+WFA+H  SMNN+N L++ DN GYAS + E+ KNK   PGQG FVAAFA +NLGDVSPNI G RCI++G  C+   STC  G    CIA GPG+DM +ST+IIG    Q A  LY +A  E+ G +   HQ+  MT++T+ L +    KTC P++GYSFAAGT DG G  +F QG    + FW+ IRD  +  PS E+  C  PKPILL TGE+S PH W P IV+ Q++ +GS+ I AIPGE TTMSGRR+R+A++         +N+TV+I+GL N Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F NL +++  +    +  GP PPF + ++  L P I D +   R FG+VLQ             V FVGANP+N  +     +F+ V+++   + +W     DA WET+F W +        G S   ++W +P + QPG+YRIRY G
Sbjct:  102 YHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQDMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEFASHGM-QNMTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQLIVPLIPSIVDRAPKGRTFGDVLQPAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYWHKG-----LLGLSNATVEWHIPDTAQPGIYRIRYFG 752
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BLAST of Neutral ceramidase vs. UniProt/SwissProt
Match: sp|Q9JHE3|ASAH2_MOUSE (Neutral ceramidase OS=Mus musculus OX=10090 GN=Asah2 PE=1 SV=1)

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 313/657 (47.64%), Postives = 421/657 (64.08%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTC-HGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRP--NLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEG 2002
            Y IG G +D TG  + +N MGY    Q   G+  RL+SRAFI A P    R  F SV+  MISQ ++ +V+K L+  +G+LY   NV LS+ H+HSGP GF QY L+ + S GF N T+  + +GI++SI  AH N+K G+IFIN G +    INRSP+SYL NP  ER RY  + DKEM VLKLV + GE++G+I+WFA+H  SMNN+N  ++ DN GYA+ +FE+ KNK   PGQG FVA FA +NLGDVSPNI G  C+++G  C+   STC +G    C+A GPG+DM EST IIG    Q A  LY +A  E+ G +   HQ+  MT+++++L      KTC P++GYSFAAGT DG    +  QGT   + FW+ +RD  +  PS E+V CQ PKPILL +GE++ PH WQP IV+ Q++ +GS+ IAAIPGELTTMSGRR R+AIKK        +++TV+IAGLSN Y+ YI T+EEYQ QRYEAASTIYGPHTL A++  F +L +++  +    +  GP PPF +++++ L P I D +   + FG+VLQ           V V FVGANP+N        +F+ V+++ +    W   + DA WET+F W  K I+    G S   I W +P +  PG+YRIRY G
Sbjct:   78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGM-KDMTVVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKHFGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW-HKGIL----GLSNATIYWHIPDTAYPGIYRIRYFG 728
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BLAST of Neutral ceramidase vs. TrEMBL
Match: A0A3S1ACQ3 (Neutral ceramidase OS=Elysia chlorotica OX=188477 GN=EGW08_003757 PE=3 SV=1)

HSP 1 Score: 781.941 bits (2018), Expect = 0.000e+0
Identity = 372/690 (53.91%), Postives = 483/690 (70.00%), Query Frame = 2 
Query:    2 MLAKLIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPP-FNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQ-TWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
            +L  +I   I +      Y  G GI+DITGPAA+VN MGYANPAQ  HGIH+R +SRAFIF++ +   R VF S+D    S  VK +VV+ L   +G +Y+ +N+ +S TH+HSG GGF QY+L+DIT+LGF  + ++A+  GIV+S+V AHN+++   I+ NSGELL +NINRSP++YL NP EER  Y YDVDK MTVLK+V  +G+ +GMI+WFAVH TSMNNTN ++S DNKGYASM+FE   N N+ PGQG FVAAFAQ+N GDVSPN  G  CIDSG PC+  TSTCHG+NE CIA GPGKDM EST+IIG  Q + A++LY +A ++  G + ++H +  MT   + L + S G TC  SMGYSFAAGTTDGPGAFDFKQGT ++N FWN IRD I+ PS ELV CQ+PKPILLATGEIS P+ WQP IV+ Q++RIG  +I A+PGE +TMSGRRMR A+     +       T +IAGL+N Y+DYI T+EEYQ QRYE ASTIYGPHTL A +  +S+L  ++ KN ++PVGP+PP  +   +S LP VIFD +   + FG V++   A  +  STV+V+FV ANPRN      +F+ V++  ++  WS EFTD+ WET++QW  ++II    GES   I WD+  +Q  G YRIR+ G +KS+  KY    G T  F V
Sbjct:    7 LLGVVILFCISIVTSDTNYYAGVGIADITGPAAEVNMMGYANPAQTSHGIHLRQFSRAFIFSASATGERIVFISIDACWASGAVKREVVRLLGAKYGGVYTVRNLVVSGTHTHSGSGGFSQYLLYDITALGFNKQGFDALVAGIVQSVVMAHNSVQPANIYFNSGELLESNINRSPSAYLNNPKEERAEYNYDVDKLMTVLKIVDAQGKGMGMISWFAVHCTSMNNTNHMLSSDNKGYASMLFEAEMNNNAAPGQGPFVAAFAQSNEGDVSPNTKGPHCIDSGKPCDILTSTCHGKNELCIAAGPGKDMFESTQIIGTNQFKKAMDLYNSATVKASGPVDFRHSYIDMTKQNVTLPDGSVGYTCKTSMGYSFAAGTTDGPGAFDFKQGTNSSNPFWNLIRDLIKDPSEELVKCQSPKPILLATGEISVPYKWQPEIVDNQLLRIGDFLIVAVPGEFSTMSGRRMRSAVLAAAIQGGMPATTTAVIAGLANEYADYITTYEEYQIQRYEGASTIYGPHTLSAFIQSYSHLATALAKNVSVPVGPSPPDLSSKQISLLPGVIFDTAPLGKHFGSVVKDAHATYTPGSTVTVEFVSANPRNDLMTNRTFLTVEKKSSEGVWSVEFTDSYWETRYQWIDESII---LGESNARISWDIGNNQAEGTYRIRHFGTSKSVLGKYTKFTGATREFEV 693
[Close]
BLAST of Neutral ceramidase vs. TrEMBL
Match: K1QV76 (Neutral ceramidase OS=Crassostrea gigas OX=29159 GN=CGI_10021248 PE=3 SV=1)

HSP 1 Score: 777.704 bits (2007), Expect = 0.000e+0
Identity = 365/674 (54.15%), Postives = 482/674 (71.51%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYEN-AKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPP-FNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFV-NQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNVI 2068
            Y+IGAGI+DITGPAA+V+ MGYANPAQ   GIH+R YSRAFI    +N++R VF S D  M SQ +K +++K LK  +G+LY+E+NVA+S TH+HSGPGGFHQY+L+DITSLGFVNET+  +  GIV SI  AHN+MK G +++N+GELL +NINRSPT+YL NP+EER +YKY+VDK MTVL+L   +   IGMINWFAVH TSMNNTN LIS DNKG A+ MFE   NK + PG+GKFVAAFAQ+N GDVSPN  G  C+D+G PC+  TSTC+GRNE C++ GPGKDM +ST+II + Q Q A  LY + + ++++G + ++H +  M+N+ +++   +  KTC P+MG+SFAAGTTDGPGAF+F QG  T N FW+F+RDF++ PSPE + CQ PKPILL TGE++ P+ W P IV+ Q+ RIG + I ++P E +TMSGRR RD+I   +K      N   +IAGL+N Y+DY+ T+EEYQ QRYE ASTIYGPHTLEA + +F+ L  ++ K    P GPTPP   +  +SFLPPV+FD++ F + FG+V+        + S+V V F+GANPRN   L  +F+ V+    +  W   FTD+ WETKF+W   +++    GESEV I WD+P +Q  G YRI+Y G AKSL  K     G +  F V+
Sbjct:   27 YIIGAGIADITGPAAEVDMMGYANPAQTSKGIHLRQYSRAFIITDSANKSRIVFVSTDSCMQSQGLKLEIIKRLKSKYGDLYNERNVAISGTHTHSGPGGFHQYLLYDITSLGFVNETFVPLVEGIVASIDKAHNSMKPGNLYVNTGELLESNINRSPTAYLNNPAEERAKYKYNVDKNMTVLRLTDAQNNGIGMINWFAVHCTSMNNTNHLISSDNKGLAAQMFEADMNKGAFPGKGKFVAAFAQSNEGDVSPNTKGPHCMDTGKPCDVPTSTCNGRNELCVSPGPGKDMYDSTRIIAKNQYQKAKILYNDPSNMKLEGPVDFRHTYVDMSNVMVQINQTTKVKTCKPAMGFSFAAGTTDGPGAFNFTQGDTTDNPFWDFVRDFLKKPSPEQLACQHPKPILLDTGEMTVPYAWSPSIVDLQIFRIGQLAIISVPAEFSTMSGRRTRDSITATLKAAGFPANTVSVIAGLTNDYADYVTTYEEYQIQRYEGASTIYGPHTLEAFIQEFNKLADALAKGTPAPKGPTPPNLLDKQLSFLPPVVFDHAPFGKSFGDVVVDADVQYKQGSSVKVTFIGANPRNNVKLEGTFLSVEMLQPDNNWKVVFTDSQWETKFEWKHTSLL---LGESEVTITWDIPQTQALGTYRIQYFGDAKSLDQKISSFTGKSTEFKVM 697
[Close]
BLAST of Neutral ceramidase vs. TrEMBL
Match: A0A2T7PXE6 (Neutral ceramidase OS=Pomacea canaliculata OX=400727 GN=C0Q70_00667 PE=3 SV=1)

HSP 1 Score: 771.541 bits (1991), Expect = 0.000e+0
Identity = 375/692 (54.19%), Postives = 482/692 (69.65%), Query Frame = 2 
Query:   23 LNILLTI------VSG--EYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFER-MKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPP-FNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVD-RFVNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
            L ILL +      VSG  E+  GAGI DITGPAA+VN MGYA P Q  +GIHMR +SRAF+FA  + E RFVF + D  M+SQ VK +V+K+LK  +G+LY+E+NV +S TH+HSGPGGFHQY+LFDITSLGFVN T+ A+  GIV+SI  AH  ++   ++I+ GELL ++INRSPT YL NP EERQ+YKYDVDK MTVL++    G  IG+INW+AVH TSMNNTN LIS DNKGYA  +FER M  + +    G+FVAAFAQ+N GDVSPN  G  C DSG PC+  TSTCHG NE CIAFGPGKDM EST+IIG  Q   AL LY +A  ++ G + ++H +  MT + + L + +  KTC P++GYSFAAGT DGPGAFDFKQGT T+N FWN +RD ++TP+ E VNC APKPILL TGEISFP++W P +V+ Q++++G  VI A+PGE TTMSGRR RDA+ + +       + + +IAGLSN Y+ Y+ATFEEYQ QRYEAASTIYGPHTL+A++  F  L  ++ K + + +GP PP       SFLP V+FD S   +KFG+V      +    S V V+FV ANPRN   L  +F+ V+ +  + +W   FTD DWET++QW   N++    GES+ +I WD+P  Q PG YRIR+ G +KS+F       G ++ F V
Sbjct:   10 LGILLCLLAYSLNVSGQAEFQAGAGIFDITGPAAEVNLMGYAKPGQTANGIHMRQFSRAFVFADKAGEKRFVFVNADSCMVSQGVKLEVIKQLKATYGDLYTERNVVISGTHTHSGPGGFHQYLLFDITSLGFVNATFEALVKGIVQSIQLAHKTLRPANLYISEGELLDSSINRSPTGYLNNPPEERQKYKYDVDKNMTVLRIDDAAGHPIGLINWYAVHCTSMNNTNGLISSDNKGYAEQLFERYMLARGNLSIPGQFVAAFAQSNEGDVSPNTKGPHCTDSGLPCDILTSTCHGENELCIAFGPGKDMFESTQIIGRNQFMKALELYSSAGKKLTGSVDFRHSYVNMTEVEVVLNSTTKVKTCKPALGYSFAAGTIDGPGAFDFKQGTNTSNPFWNAVRDVLKTPTEEQVNCHAPKPILLDTGEISFPYLWHPQVVDVQLLKLGQFVIIAVPGEFTTMSGRRTRDAVVQTLISNGLPLDTSSVIAGLSNDYTHYVATFEEYQVQRYEAASTIYGPHTLQAYIQNFEILAEALAKGKPVSLGPNPPNLLGQQWSFLPGVLFDSSPVGKKFGDVKTDAEPSYQPGSVVQVRFVSANPRNDLRLNGTFLTVEQKQESGSWRVIFTDRDWETRYQWINDNLL---LGESDAIIRWDIPEGQTPGTYRIRHFGTSKSIFGSLTSFEGSSSLFMV 698
[Close]
BLAST of Neutral ceramidase vs. TrEMBL
Match: A0A0B7A399 (Neutral ceramidase OS=Arion vulgaris OX=1028688 GN=ORF94828 PE=3 SV=1)

HSP 1 Score: 769.615 bits (1986), Expect = 0.000e+0
Identity = 358/675 (53.04%), Postives = 475/675 (70.37%), Query Frame = 2 
Query:   47 SGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPP-FNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQ-TWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNV 2065
            S  Y +G GI D+TGP+A +N MGYANP QI HGIHMR +SRAFIFA+     R VF S+D       VK +VVK+LK +FGN+Y+E+NV +S TH+HSG GGF QY+L+DIT+ GF ++ Y A+  G+++SI  AH+N+K   I++N G+LL +NINRSPT+YL NP+ ER +Y YDVDK +TVLK+V   G  +GMI+WFAVH TSMNNTN+L+S DNKGYAS +FE+  N  + PGQG FVAAFAQ+NLGDVSPN  G  C+DSGNPC++ TSTCHG+NE C+AFGPGKDM EST+IIG  Q   AL+LY +A  ++ G + ++H +  M    + L + S  + C P+MGYSFAAGTTDGPGAFDFKQG+ +++  WNF+RD I+ P+ + V C +PKPILL TGEI+ P++WQP IV+ Q++R+G  VI A+PGE +TM+GRRMR+ +   +  +   EN T +IAGL+N YSDYI TFEEYQ QRYE ASTIYGPHTLEAHL  ++ L+  M   +  P+GP PP      +SF+P VIFD + F + FG VL     + ++ STVSV FV ANPRN      +F+ V+   N  +W  ++TD+ W+T+F W + + I    GESE  I WD+P  Q  G YRI++ G +KSLF +     G +  F++
Sbjct:   26 SANYYVGVGIGDVTGPSADINMMGYANPGQISHGIHMREFSRAFIFANTPTSDRVVFVSIDACWAPGAVKQEVVKQLKALFGNIYTERNVVISGTHTHSGSGGFSQYLLYDITAFGFNHQGYQALVDGVIQSIKQAHSNLKPADIYLNVGQLLDSNINRSPTAYLNNPAAERAQYDYDVDKNITVLKIVDGAGVGMGMISWFAVHCTSMNNTNQLLSSDNKGYASQLFEKEMNGAAHPGQGPFVAAFAQSNLGDVSPNTQGPHCLDSGNPCDRDTSTCHGKNELCVAFGPGKDMFESTEIIGNNQFLKALDLYNSANFKLNGPVDFRHTYVDMPKQNVTLTDGSKVQLCKPAMGYSFAAGTTDGPGAFDFKQGSNSSSPIWNFVRDLIKAPTAQQVKCHSPKPILLDTGEITLPYLWQPQIVDHQILRVGEFVIIAVPGEFSTMAGRRMRNTVYSTLVGKGFSENTTTVIAGLANEYSDYITTFEEYQIQRYEGASTIYGPHTLEAHLQSYTLLSTQMADGQPSPLGPDPPNMIGKQISFVPGVIFDTAPFGKHFGSVLVDAQPSYTQGSTVSVTFVSANPRNNLKTNDTFLTVEHKTNNGSWVVDYTDSYWQTRFYWIRTSTI---LGESEAKITWDIPADQALGTYRIQHYGTSKSLFGQLTPFVGNSREFSI 697
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BLAST of Neutral ceramidase vs. TrEMBL
Match: A0A2R2MQ33 (Neutral ceramidase OS=Lingula unguis OX=7574 GN=LOC106168246 PE=3 SV=1)

HSP 1 Score: 769.615 bits (1986), Expect = 0.000e+0
Identity = 368/699 (52.65%), Postives = 485/699 (69.38%), Query Frame = 2 
Query:    2 MLAKLIALNILLTIVSGE------YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGP-TPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQ-TWSTEFTDADWETKFQWFQKN-IIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFS-KYQGIHGVTNSFNVI 2068
            +LA+ I     L  ++ +      Y IGAGI+D+TGPAA+VN MGYANP+Q  HGIHMRLYSRAFI A  +  +R VF +VD  MIS I+K KV  +LK ++  LY E NV +S TH+HSGPGG+H+Y+LFD+TSLGFV E+ + +  GIV+SI NAHNNM  G I+IN+G LL +NINRSPT+YL NP  E+ +Y Y+VDK+MTVLK V   G+ +GMI+WFAVH TSMNNTN  ISGDNKGYA+ +FER  N  S PG+GKF+AAFAQ+N GDVSPN  GA CID+G PC+  TSTC+G+ E C+A GPGKDM ESTKIIG++Q + A  L++ A   + G + Y+HQF  M+N  +++    +  TC P+MGYSFAAGTTDGPG+FDF QGT T N FWNF+R+ +  PS   + CQ PKPILL TGE+SFP+ WQP +V TQ++RIG ++I A+PGE +TMSGRR+R+A+ ++  ++    N   ++AGLSN+Y+DY+ T+EEYQ QRYE ASTIYGPHTL A + +F  LT +M +  T+  GP TP F   ++SFL PV+ D     R FG+V++QP +   +   V V F+ ANPRN      SF+ V++  +  +W T FTDADWET + W + + I+A    E    I W +P  Q  G YRIR+ G AK + +   +   G T SF V+
Sbjct:    5 VLARHILFACFLACLATDCQGVANYYIGAGIADVTGPAAEVNMMGYANPSQKSHGIHMRLYSRAFIIADAAKTSRVVFVNVDACMISDIMKKKVANKLKTLYNGLYDETNVCVSGTHTHSGPGGYHRYVLFDVTSLGFVEESLDPLVDGIVKSIQNAHNNMVAGNIYINTGVLLNSNINRSPTAYLNNPPGEKAKYNYNVDKDMTVLKFVDGNGQPLGMISWFAVHCTSMNNTNGYISGDNKGYAAQLFEREMNPGSLPGEGKFIAAFAQSNEGDVSPNTKGAHCIDTGKPCDVSTSTCNGKPELCVASGPGKDMYESTKIIGKKQYEKAKELFDGANTLLTGPVDYRHQFVDMSNQKVQIDATHYATTCPPAMGYSFAAGTTDGPGSFDFTQGTTTGNPFWNFVRNILSKPSRAQIQCQHPKPILLNTGEMSFPYPWQPSVVPTQILRIGELMILAVPGEFSTMSGRRLRNAVTEIATQQGLPSNTKTVVAGLSNSYADYVVTYEEYQIQRYEGASTIYGPHTLLAFIQKFKGLTTAMAQGSTLSSGPSTPNFPSKLISFLTPVLHDSPPLFRSFGDVIKQPKSKYIQGDVVKVSFISANPRNNVMSGKSFLTVEKKQSDGSWKTVFTDADWETSYYWKRTSTILALSVSE----ISWKIPDQQPKGTYRIRHFGTAKKMITGSLKSFVGSTKSFQVV 699
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BLAST of Neutral ceramidase vs. Ensembl Cavefish
Match: asah2 (neutral ceramidase [Source:NCBI gene;Acc:103021762])

HSP 1 Score: 623.239 bits (1606), Expect = 0.000e+0
Identity = 318/681 (46.70%), Postives = 434/681 (63.73%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFL--PPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNL-IYSFIFVDRFVN--QTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKS--LFSKYQGIHGVTNSFNV 2065
            YLIG G +D TGP A V  MGYANP Q   GIH RL+SRAFI    S   R VF + D GM+SQ ++ +V+KEL+  +G+LY ++NV LS TH+HSG  G+ QY LF ITS G++  +   +  GIV+SI  AH NM+ GRIF++ GE++G+N+NRSP SYL NP EER RYK + D+++  LK   ++G+ +G+ +WFAVH  SMN TN+++S DN GYAS +FE+ KN    PG+G FVAAFA AN GDV+PN  G  CI++G  C+   S+C  G    C+AFGPG D+ +ST+IIGE   + A  LY +AK E++G +   HQ+  MT++T++L +    KTC P++G+SFAAGT DG G  +F QG V  + FW+ IRD  +  PS E+  C  PKPIL +TGE+++P  W P IV+ Q++ IGSV I A+PGE TTMSGRR+R+ +K+ +++     N  V+I GL N Y+ YI T+EEYQ QRYE ASTIYGPHTL A++  F  L R++ + +   +P GP PPF  ++ S L  PPV  D S  N  FG+VLQQ           SV FV  NPRN   +   +F+ V++F N   TW    TDA WET+F W +         +S   ++W +P S + G YRIR+ G  K   +F       G +++F V
Sbjct:   60 YLIGVGRADCTGPVADVPLMGYANPDQTAGGIHTRLFSRAFIVDDGSK--RVVFVNADIGMVSQRLRLEVLKELELKYGDLYRQENVILSGTHTHSGLAGYFQYTLFIITSKGYIKSSIQPIVNGIVKSIDVAHKNMRPGRIFVSKGEIVGSNLNRSPHSYLNNPEEERNRYKSNTDEQIVTLKFTDLDGDGMGVFSWFAVHPVSMNYTNRMVSSDNLGYASYLFEQEKNIGYLPGEGPFVAAFATANSGDVTPNTRGPYCINTGERCDYLNSSCPIGGTRMCVAFGPGNDIFDSTRIIGENIYRKAKELYGSAKQELRGSVHAAHQWVNMTDVTVQLDSTHSVKTCKPALGHSFAAGTIDGGGDLNFTQGAVEGDPFWDGIRDALLGAPSKEIQECHNPKPILFSTGEMNWPFPWHPDIVDVQIITIGSVAIVAVPGEATTMSGRRIREGVKQELEKNAAFTNSEVVITGLCNIYTHYITTYEEYQVQRYEGASTIYGPHTLSAYIQHFRGLARAIAEGKVGELPKGPEPPFFSNLFSLLGEPPV--DRSPENTTFGDVLQQVHPVYKVGEVASVTFVAGNPRNSGEMRDKTFVTVEKFHNSTSTWEVVHTDASWETRFYWIKGT-----GNQSNATVEWHIPLSAESGTYRIRHFGHYKQRVIFPVITAYEGSSHTFRV 731
[Close]
BLAST of Neutral ceramidase vs. Ensembl Cavefish
Match: asah2 (neutral ceramidase [Source:NCBI gene;Acc:103021762])

HSP 1 Score: 599.356 bits (1544), Expect = 0.000e+0
Identity = 301/619 (48.63%), Postives = 406/619 (65.59%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFL--PPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNL-IYSFIFVDRFVN--QTWSTEFTDADWETK 1885
            YLIG G +D TGP A V  MGYANP Q   GIH RL+SRAFI    S   R VF + D GM+SQ ++ +V+KEL+  +G+LY ++NV LS TH+HSG  G+ QY LF ITS G++  +   +  GIV+SI  AH NM+ GRIF++ GE++G+N+NRSP SYL NP EER RYK + D+++  LK   ++G+ +G+ +WFAVH  SMN TN+++S DN GYAS +FE+ KN    PG+G FVAAFA AN GDV+PN  G  CI++G  C+   S+C  G    C+AFGPG D+ +ST+IIGE   + A  LY +AK E++G +   HQ+  MT++T++L +    KTC P++G+SFAAGT DG G  +F QG V  + FW+ IRD  +  PS E+  C  PKPIL +TGE+++P  W P IV+ Q++ IGSV I A+PGE TTMSGRR+R+ +K+ +++     N  V+I GL N Y+ YI T+EEYQ QRYE ASTIYGPHTL A++  F  L R++ + +   +P GP PPF  ++ S L  PPV  D S  N  FG+VLQQ           SV FV  NPRN   +   +F+ V++F N   TW    TDA WET+
Sbjct:   60 YLIGVGRADCTGPVADVPLMGYANPDQTAGGIHTRLFSRAFIVDDGSK--RVVFVNADIGMVSQRLRLEVLKELELKYGDLYRQENVILSGTHTHSGLAGYFQYTLFIITSKGYIKSSIQPIVNGIVKSIDVAHKNMRPGRIFVSKGEIVGSNLNRSPHSYLNNPEEERNRYKSNTDEQIVTLKFTDLDGDGMGVFSWFAVHPVSMNYTNRMVSSDNLGYASYLFEQEKNIGYLPGEGPFVAAFATANSGDVTPNTRGPYCINTGERCDYLNSSCPIGGTRMCVAFGPGNDIFDSTRIIGENIYRKAKELYGSAKQELRGSVHAAHQWVNMTDVTVQLDSTHSVKTCKPALGHSFAAGTIDGGGDLNFTQGAVEGDPFWDGIRDALLGAPSKEIQECHNPKPILFSTGEMNWPFPWHPDIVDVQIITIGSVAIVAVPGEATTMSGRRIREGVKQELEKNAAFTNSEVVITGLCNIYTHYITTYEEYQVQRYEGASTIYGPHTLSAYIQHFRGLARAIAEGKVGELPKGPEPPFFSNLFSLLGEPPV--DRSPENTTFGDVLQQVHPVYKVGEVASVTFVAGNPRNSGEMRDKTFVTVEKFHNSTSTWEVVHTDASWETR 674
[Close]
BLAST of Neutral ceramidase vs. Ensembl Cavefish
Match: asah2 (neutral ceramidase [Source:NCBI gene;Acc:103021762])

HSP 1 Score: 596.275 bits (1536), Expect = 0.000e+0
Identity = 301/619 (48.63%), Postives = 406/619 (65.59%), Query Frame = 2 
Query:   56 YLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFL--PPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNL-IYSFIFVDRFVN--QTWSTEFTDADWETK 1885
            YLIG G +D TGPAA V  MGYAN  Q   GIH RL+SRAFI    S   R VF + + GMISQ ++ +V+KEL+  +G+LY ++NV LS TH+HSG  G+ QY LF ITS G++  +   +  GIV+SI  AH NM+ GRIF++ GE++G+N+NRSP SYL NP EER RYK + D+++  LK   ++G+ +G+ +WFAVH  SMN TN+++S DN GYAS +FE+ KN    PG+G FVAAFA AN GDV+PN  G  CI++G  C+   S+C  G    C+AFGPG D+ +ST+IIGE   + A  LY +AK E++G +   HQ+  MT++T++L +    KTC P++G+SFAAGT DG G  +F QG V  + FW+ IRD  +  PS E+  C  PKPIL +TGE+++P  W P IV+ Q++ IGSV I A+PGE TTMSGRR+R+ +K+ +++     N  V+I GL N Y+ YI T+EEYQ QRYE ASTIYGPHTL A++  F  L R++ + +   +P GP PPF  ++ S L  PPV  D S  N  FG+VLQQ           SV FV  NPRN   +   +F+ V++F N   TW    TDA WET+
Sbjct:   60 YLIGLGRADCTGPAADVPLMGYANLEQTAGGIHTRLFSRAFIVDDGSK--RVVFVTAEIGMISQRLRLEVLKELELKYGDLYRQENVILSGTHTHSGLAGYFQYTLFIITSKGYIKSSIQPIVNGIVKSIDVAHKNMRPGRIFVSKGEIVGSNLNRSPHSYLNNPEEERNRYKSNTDEQIVTLKFTDLDGDGMGVFSWFAVHPVSMNYTNRMVSSDNLGYASYLFEQEKNIGYLPGEGPFVAAFATANSGDVTPNTRGPYCINTGERCDYLNSSCPIGGTRMCVAFGPGNDIFDSTRIIGENIYRKAKELYGSAKQELRGSVHAAHQWVNMTDVTVQLDSTHSVKTCKPALGHSFAAGTIDGGGDLNFTQGAVEGDPFWDGIRDALLGAPSKEIQECHNPKPILFSTGEMNWPFPWHPDIVDVQIITIGSVAIVAVPGEATTMSGRRIREGVKQELEKNAAFTNSEVVITGLCNIYTHYITTYEEYQVQRYEGASTIYGPHTLSAYIQHFRGLARAIAEGKVGELPKGPEPPFFSNLFSLLGEPPV--DRSPENTTFGDVLQQVHPVYKVGEVASVTFVAGNPRNSGEMRDKTFVTVEKFHNSTSTWEVVHTDASWETR 674
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BLAST of Neutral ceramidase vs. Ensembl Sea Lamprey
Match: asah2 (N-acylsphingosine amidohydrolase 2 [Source:NCBI gene;Acc:493602])

HSP 1 Score: 482.641 bits (1241), Expect = 2.291e-162
Identity = 281/680 (41.32%), Postives = 383/680 (56.32%), Query Frame = 2 
Query:   59 LIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQ----------------GKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIR-TPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETI------PVGPTPPFNEDVVSFLPPVIFDYS---GFNRKFGEVLQQPPANVSR-NSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAK 2011
            +IG G +D TGPA +V  MGYA+P+Q+  GIH RLYSRAFI A  S   R VF SVD GMISQ V+ +V+  LK  +G+ Y+E NV LS TH+HSGPGG+ QY LF + S GF+   + A+T+GI+ SI  AH NMK G +F++ GE+  +NINRSP SYL NP+ ER RYK + D+++ +LKLV  EG  IG++ WFAVH  SMN TNKLIS DN GYA+ +FE+  N    PG+                G +    + A   D  P I+    I + +P                   P   M   T  I + +      L+      + G +    Q+  MT++ ++  N +  KTC P++GYSFAAGTTDGPG+ +F QGT   N+FW+ IRDF+   PS E   CQAPKPIL  TG+++ P  W P IV+ Q++  G   + A+PGE TTMSGRR+R+A+KK ++     ++V V+I+GLSN Y+ Y+ TFEEYQ QRYE ASTIYGPHTL A++  +S L R+ + N T+      P  P+          LP    D     G         Q+P  +++R + +V+          R     +F+ V+    Q   W    TDA WET+F W  K I    FG S   + W +P S + G YR+R+ G  K
Sbjct:    1 MIGVGRADCTGPAVEVAMMGYASPSQVSKGIHTRLYSRAFIAADFSGN-RVVFVSVDVGMISQKVRLEVLMRLKDKYGSQYNESNVVLSGTHTHSGPGGYFQYSLFVVASQGFIKPCFEAITSGILHSIDIAHGNMKPGNLFLSRGEVKESNINRSPFSYLNNPAAERSRYKTNTDEDILLLKLVDQEGTAIGVVTWFAVHAVSMNLTNKLISSDNMGYAAYLFEQEMNPGFLPGEVSASMYNNSFVDMCVHGYYTGVHSTARTKDKIPAID-TPIIYTYSPVR-----------------PLLCMFTHTLTIQQLKSFKTNELFSGEGRTLTGPVNSAQQWVNMTDVVVRF-NSTHAKTCKPALGYSFAAGTTDGPGSLNFTQGTTEGNAFWDAIRDFLTGPPSNETTECQAPKPILFDTGDMTRPLPWHPEIVDVQMVTFGDFAVLAVPGEFTTMSGRRLREAVKKELQRNEFFQSVDVMISGLSNVYTHYVTTFEEYQIQRYEGASTIYGPHTLAAYVQLYSGLARA-IANTTVEEARRKPSTPSYRLATLWWRCLPASRHDREPSLGSCAIAIGCAQEPLLHITRSDPSVTTPISSGGHFIRGG---TFLTVEWHNPQAGNWRVIHTDASWETRFIW-DKGI----FGRSNATVYWAIPASAEAGKYRVRHFGHYK 651
[Close]
BLAST of Neutral ceramidase vs. Ensembl Medaka
Match: asah2 (N-acylsphingosine amidohydrolase 2 [Source:NCBI gene;Acc:101165218])

HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0
Identity = 311/664 (46.84%), Postives = 435/664 (65.51%), Query Frame = 2 
Query:   47 SGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCH-GRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRD-FIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNET--IPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNL-IYSFIFVDRFVNQT--WSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSL 2017
            S  YLIG G +D TG AA++  MGYAN  Q   GIH RL+SRAFI        R +F + D GMISQ ++ +V+K L++ +G+LY  +NV +S TH+H GPGG+ QY LF IT  G+V E+   +  GIV+SI  AH NM+ GRI++N GEL+ +++NRSP SY+ NP +ER+RY+ + DK++ VLK   ++G+ IGMI+WFAVH  SMN TN+++S DN GYAS + E+ KN    PGQG FVA F+ +NLGDVSPN  G  C+++G  C+   S+C  G  + C AFGPG+DM EST+IIG      A  LY NA  E+ G +Q+ HQ+  MT++T++L +   G+TC P++G+SFAAGTTDG G  +F QG V  + FW+ IRD  +  PS +  +C  PKPIL +TGE+++P  W P IV+ Q++ IGSV + AIPGE+TTM+GRR+R A+KK ++   + +N  V+IAGLSN Y+ YI TFEEYQ QRYE ASTI+GPHT  A+L +F+NL R++ +++   +PVGP PPF  ++ + +    +D    N  FG+VL Q  +   +   VSV FV  NPR+  ++   +F+ V+ + N T  W     DA WET+F W + +        S   ++W +P S   GLYRIR+ G  K L
Sbjct:   47 SKTYLIGVGRADCTGSAAEIPMMGYANSQQKAAGIHTRLFSRAFIV--DDGNQRVLFVTADIGMISQRLRLEVLKALQEKYGDLYRRENVVMSGTHTHCGPGGYFQYTLFMITGNGYVKESTEPLVNGIVKSIDIAHRNMRPGRIYVNKGELVDSSLNRSPHSYMNNPEDERKRYEKNTDKQVVVLKFTDLDGDGIGMISWFAVHAVSMNFTNRMVSSDNMGYASYLLEQDKNFGELPGQGSFVAGFSSSNLGDVSPNTRGPHCMNTGLLCDYLNSSCPVGGTKMCKAFGPGEDMFESTRIIGHNIFMKAKELYANAVHEVTGSVQFAHQWVNMTDVTVQLNDTHTGRTCKPALGHSFAAGTTDGGGDLNFTQGAVEGDPFWDGIRDALLGPPSNQTKDCHHPKPILFSTGEMNWPLPWHPSIVDVQIITIGSVAVLAIPGEMTTMAGRRLRAAVKKELEAAEKFKNAEVVIAGLSNVYTHYITTFEEYQIQRYEGASTIFGPHTFAAYLQKFTNLARAIAQDKVSELPVGPEPPFFTNLFNLMAAAAYDAKPENTSFGDVLDQVNSIYRQGDVVSVTFVAGNPRHSGDIRDKTFVTVEMYDNLTDSWGVVHNDASWETRFHWLKGS-----NRRSNATVEWHIPSSAPSGLYRIRHFGHYKQL 703
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BLAST of Neutral ceramidase vs. Planmine SMEST
Match: SMESG000003228.1 (SMESG000003228.1)

HSP 1 Score: 1434.47 bits (3712), Expect = 0.000e+0
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 2 
Query:    2 MLAKLIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNVI 2068
            MLAKLIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNVI
Sbjct:    1 MLAKLIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGIHMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLYSEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVNAHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTVLKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKNSRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKCIAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTNLTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIRDFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIAAIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFEEYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNEDVVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPNLIYSFIFVDRFVNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDWDVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNVI 689
The following BLAST results are available for this feature:
BLAST of Neutral ceramidase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ASAH20.000e+048.40N-acylsphingosine amidohydrolase 2 [Source:HGNC Sy... [more]
ASAH20.000e+048.40N-acylsphingosine amidohydrolase 2 [Source:HGNC Sy... [more]
ASAH20.000e+046.88N-acylsphingosine amidohydrolase 2 [Source:HGNC Sy... [more]
ASAH20.000e+046.88N-acylsphingosine amidohydrolase 2 [Source:HGNC Sy... [more]
ASAH20.000e+048.05N-acylsphingosine amidohydrolase 2 [Source:HGNC Sy... [more]
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BLAST of Neutral ceramidase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neutral ceramidase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CDase0.000e+046.70gene:FBgn0039774 transcript:FBtr0331357[more]
CDase0.000e+046.70gene:FBgn0039774 transcript:FBtr0085648[more]
CDase0.000e+046.70gene:FBgn0039774 transcript:FBtr0085645[more]
CDase0.000e+046.70gene:FBgn0039774 transcript:FBtr0085646[more]
CDase0.000e+046.70gene:FBgn0039774 transcript:FBtr0085647[more]
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BLAST of Neutral ceramidase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
asah20.000e+045.47N-acylsphingosine amidohydrolase 2 [Source:NCBI ge... [more]
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BLAST of Neutral ceramidase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
asah20.000e+046.84N-acylsphingosine amidohydrolase (non-lysosomal ce... [more]
asah20.000e+046.69N-acylsphingosine amidohydrolase (non-lysosomal ce... [more]
asah20.000e+046.54N-acylsphingosine amidohydrolase (non-lysosomal ce... [more]
grin2c0.000e+046.53glutamate receptor, ionotropic, N-methyl D-asparta... [more]
asah20.000e+043.22N-acylsphingosine amidohydrolase (non-lysosomal ce... [more]
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BLAST of Neutral ceramidase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Asah20.000e+047.64N-acylsphingosine amidohydrolase 2 [Source:MGI Sym... [more]
Asah20.000e+047.64N-acylsphingosine amidohydrolase 2 [Source:MGI Sym... [more]
Asah20.000e+047.64N-acylsphingosine amidohydrolase 2 [Source:MGI Sym... [more]
Asah20.000e+047.64N-acylsphingosine amidohydrolase 2 [Source:MGI Sym... [more]
Asah20.000e+047.64N-acylsphingosine amidohydrolase 2 [Source:MGI Sym... [more]
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BLAST of Neutral ceramidase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q29C43|NCASE_DROPS0.000e+046.98Neutral ceramidase OS=Drosophila pseudoobscura pse... [more]
sp|Q9VA70|NCASE_DROME0.000e+046.70Neutral ceramidase OS=Drosophila melanogaster OX=7... [more]
sp|Q55G11|NCSEB_DICDI0.000e+050.44Neutral ceramidase B OS=Dictyostelium discoideum O... [more]
sp|Q9NR71|ASAH2_HUMAN0.000e+048.40Neutral ceramidase OS=Homo sapiens OX=9606 GN=ASAH... [more]
sp|Q9JHE3|ASAH2_MOUSE0.000e+047.64Neutral ceramidase OS=Mus musculus OX=10090 GN=Asa... [more]
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BLAST of Neutral ceramidase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S1ACQ30.000e+053.91Neutral ceramidase OS=Elysia chlorotica OX=188477 ... [more]
K1QV760.000e+054.15Neutral ceramidase OS=Crassostrea gigas OX=29159 G... [more]
A0A2T7PXE60.000e+054.19Neutral ceramidase OS=Pomacea canaliculata OX=4007... [more]
A0A0B7A3990.000e+053.04Neutral ceramidase OS=Arion vulgaris OX=1028688 GN... [more]
A0A2R2MQ330.000e+052.65Neutral ceramidase OS=Lingula unguis OX=7574 GN=LO... [more]
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BLAST of Neutral ceramidase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
asah20.000e+046.70neutral ceramidase [Source:NCBI gene;Acc:103021762... [more]
asah20.000e+048.63neutral ceramidase [Source:NCBI gene;Acc:103021762... [more]
asah20.000e+048.63neutral ceramidase [Source:NCBI gene;Acc:103021762... [more]
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BLAST of Neutral ceramidase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
asah22.291e-16241.32N-acylsphingosine amidohydrolase 2 [Source:NCBI ge... [more]
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BLAST of Neutral ceramidase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neutral ceramidase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Neutral ceramidase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
asah20.000e+046.84N-acylsphingosine amidohydrolase 2 [Source:NCBI ge... [more]
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BLAST of Neutral ceramidase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000003228.10.000e+0100.00SMESG000003228.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014265 ID=SMED30014265|Name=Neutral ceramidase|organism=Schmidtea mediterranea sexual|type=transcript|length=2135bp
AATGTTGGCCAAATTGATAGCGTTAAATATTTTGCTAACAATTGTTTCCG
GTGAATATTTGATAGGAGCCGGAATAAGTGACATTACTGGGCCTGCTGCC
CAGGTTAATTTTATGGGCTATGCCAATCCGGCTCAAATTGGCCATGGAAT
ACACATGCGACTCTACAGTAGAGCATTCATATTTGCAAGTCCCTCAAATG
AGACCCGATTTGTGTTTGCCAGTGTTGATTGTGGAATGATATCCCAAATC
GTTAAAACAAAAGTTGTAAAAGAGCTGAAAAAAATCTTCGGGAATTTGTA
TTCGGAACAAAATGTAGCGTTGAGTTCTACTCACAGTCATTCTGGACCGG
GAGGATTTCATCAGTACATCCTCTTCGATATCACATCATTGGGATTCGTG
AATGAAACATACAACGCCATGACAACTGGCATTGTTCGGAGTATTGTAAA
TGCACATAATAACATGAAAAAAGGAAGAATATTTATCAATTCTGGGGAGT
TATTGGGAGCCAATATCAATAGGAGTCCTACATCATACTTAGCTAATCCC
TCTGAAGAAAGACAAAGATACAAATACGATGTTGACAAAGAGATGACAGT
ATTGAAGTTAGTAAGCATGGAAGGTGAGGAAATAGGGATGATCAATTGGT
TCGCTGTCCACGGAACCAGTATGAACAATACAAACAAATTAATCAGTGGT
GACAATAAGGGATATGCGTCAATGATGTTTGAACGAATGAAAAATAAGAA
CAGCCGACCTGGACAAGGTAAATTTGTTGCCGCATTTGCTCAAGCAAATC
TCGGAGATGTCTCACCAAACATAAATGGTGCTCGGTGCATTGACTCGGGG
AATCCTTGTGAAAAATTCACAAGCACTTGCCACGGTCGAAATGAAAAGTG
TATTGCATTTGGACCTGGAAAGGATATGATTGAAAGCACGAAAATTATTG
GAGAAAGACAATTACAAGCAGCTCTTAATTTATATGAAAATGCAAAAATT
GAGATCAAAGGTCTGATTCAATATAAACATCAGTTCAAACAAATGACAAA
TTTGACAATAAAATTGGCAAATGATAGCTGGGGCAAAACATGCTTGCCAT
CGATGGGGTACAGTTTCGCCGCAGGAACGACCGACGGACCCGGCGCTTTT
GATTTTAAACAAGGTACGGTGACCACTAATTCCTTTTGGAACTTCATTAG
AGATTTTATTCGTACTCCTAGTCCTGAGCTTGTAAATTGTCAGGCCCCTA
AGCCTATTTTACTAGCAACTGGTGAAATTTCGTTTCCTCATATATGGCAA
CCCCATATCGTAGAAACACAAGTTATGAGAATAGGATCAGTAGTCATAGC
TGCAATTCCCGGAGAATTGACAACGATGTCTGGCAGACGCATGAGAGACG
CAATAAAAAAAGTAATGAAAGAAGAAAATCAAGGCGAAAATGTAACAGTT
ATAATAGCTGGCTTGTCAAATACGTATTCAGATTATATCGCTACATTTGA
GGAATATCAGAAACAGAGGTATGAAGCAGCATCAACCATATACGGTCCCC
ATACACTTGAAGCACATTTATCTCAATTTTCCAATTTAACTAGAAGCATG
GTCAAAAATGAAACAATACCAGTCGGACCGACGCCTCCCTTTAATGAAGA
TGTTGTGAGCTTTCTGCCTCCTGTAATTTTTGACTATTCTGGTTTTAATC
GAAAATTTGGTGAGGTCTTACAGCAGCCTCCGGCAAATGTTTCTAGAAAT
AGCACGGTATCAGTGAAATTTGTTGGAGCAAATCCAAGGAATCGTCCCAA
TTTAATTTATTCGTTCATTTTTGTCGACAGATTCGTTAATCAAACTTGGT
CCACTGAATTCACAGACGCGGACTGGGAAACTAAATTCCAATGGTTTCAG
AAAAATATAATCGCATTTTTTTTCGGTGAAAGTGAAGTGGTCATAGATTG
GGATGTTCCTGGCTCCCAGCAACCCGGTCTTTACAGGATCAGATATGAAG
GTGTAGCGAAATCATTATTTTCTAAATATCAAGGCATTCACGGAGTCACA
AATTCATTCAATGTTATCTGATGTCTGGAGTTGTTGATATTATATAGACA
TTAATAAATTTAATATAACTAATTTTATTGGTGAC
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protein sequence of SMED30014265-orf-1

>SMED30014265-orf-1 ID=SMED30014265-orf-1|Name=SMED30014265-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=690bp
MLAKLIALNILLTIVSGEYLIGAGISDITGPAAQVNFMGYANPAQIGHGI
HMRLYSRAFIFASPSNETRFVFASVDCGMISQIVKTKVVKELKKIFGNLY
SEQNVALSSTHSHSGPGGFHQYILFDITSLGFVNETYNAMTTGIVRSIVN
AHNNMKKGRIFINSGELLGANINRSPTSYLANPSEERQRYKYDVDKEMTV
LKLVSMEGEEIGMINWFAVHGTSMNNTNKLISGDNKGYASMMFERMKNKN
SRPGQGKFVAAFAQANLGDVSPNINGARCIDSGNPCEKFTSTCHGRNEKC
IAFGPGKDMIESTKIIGERQLQAALNLYENAKIEIKGLIQYKHQFKQMTN
LTIKLANDSWGKTCLPSMGYSFAAGTTDGPGAFDFKQGTVTTNSFWNFIR
DFIRTPSPELVNCQAPKPILLATGEISFPHIWQPHIVETQVMRIGSVVIA
AIPGELTTMSGRRMRDAIKKVMKEENQGENVTVIIAGLSNTYSDYIATFE
EYQKQRYEAASTIYGPHTLEAHLSQFSNLTRSMVKNETIPVGPTPPFNED
VVSFLPPVIFDYSGFNRKFGEVLQQPPANVSRNSTVSVKFVGANPRNRPN
LIYSFIFVDRFVNQTWSTEFTDADWETKFQWFQKNIIAFFFGESEVVIDW
DVPGSQQPGLYRIRYEGVAKSLFSKYQGIHGVTNSFNVI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005576extracellular region
GO:0005737cytoplasm
GO:0045202synapse
GO:0098793presynapse
Vocabulary: INTERPRO
TermDefinition
IPR038445NCDase_C_sf
IPR006823Ceramidase_alk
IPR031329NEUT/ALK_ceramidase_N
IPR031331NEUT/ALK_ceramidase_C
Vocabulary: biological process
TermDefinition
GO:0006629lipid metabolic process
GO:0006665sphingolipid metabolic process
GO:0007268chemical synaptic transmission
GO:0016079synaptic vesicle exocytosis
GO:0031629synaptic vesicle fusion to presynaptic active zone membrane
GO:0035187hatching behavior
GO:0045494photoreceptor cell maintenance
GO:0046514ceramide catabolic process
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
GO:0017040ceramidase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR031329Neutral/alkaline non-lysosomal ceramidase, N-terminalPFAMPF04734Ceramidase_alkcoord: 19..523
e-value: 7.5E-229
score: 760.5
IPR031331Neutral/alkaline non-lysosomal ceramidase, C-terminalPFAMPF17048Ceramidse_alk_Ccoord: 526..688
e-value: 3.4E-42
score: 144.2
IPR038445Neutral ceramidase, C-terminal domain superfamilyGENE3DG3DSA:2.60.40.2300coord: 558..689
e-value: 4.8E-35
score: 122.2
NoneNo IPR availablePANTHERPTHR12670:SF1NEUTRAL CERAMIDASE-RELATEDcoord: 9..688
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.867
IPR006823Neutral/alkaline nonlysosomal ceramidasePANTHERPTHR12670CERAMIDASEcoord: 9..688