Lysosomal aspartic protease
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014243 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 14
Homology
BLAST of Lysosomal aspartic protease vs. Ensembl Human
Match: CTSD (cathepsin D [Source:HGNC Symbol;Acc:HGNC:2529]) HSP 1 Score: 373.244 bits (957), Expect = 3.658e-126 Identity = 197/384 (51.30%), Postives = 247/384 (64.32%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFMK------------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV-------ATA----KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISML-GKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + + + PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A+A KV Q FGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLVD IFSFYL+RD + +PGGE++ GG+D YY+G +Y VT K YWQ + + + G T C C AI DTGTSL+VGP + ++ I G +I C VS LP I +++ G+ + L P DY LK T+C+SGF + SG P +ILGDVFIGRYYT FD N+R+GFA
Sbjct: 22 VRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKAGKTLCLSGFMGMDIPPPSG-PLWILGDVFIGRYYTVFDRDNNRVGFA 404
BLAST of Lysosomal aspartic protease vs. Ensembl Human
Match: CTSD (cathepsin D [Source:HGNC Symbol;Acc:HGNC:2529]) HSP 1 Score: 369.392 bits (947), Expect = 1.441e-124 Identity = 197/387 (50.90%), Postives = 248/387 (64.08%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFMK------------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV-------ATA----KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISML-GKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + + + PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A+A KV Q FGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLVD IFSFYL+RD + +PGGE++ GG+D YY+G +Y VT K YWQ + + + G T C C AI DTGTSL+VGP + ++ I G +I C VS LP I +++ G+ + L P DY LK+ T+C+SGF + SG P +ILGDVFIGRYYT FD N+R+GFA
Sbjct: 22 VRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSG-PLWILGDVFIGRYYTVFDRDNNRVGFA 407
BLAST of Lysosomal aspartic protease vs. Ensembl Human
Match: CTSD (cathepsin D [Source:HGNC Symbol;Acc:HGNC:2529]) HSP 1 Score: 362.459 bits (929), Expect = 5.908e-122 Identity = 195/386 (50.52%), Postives = 247/386 (63.99%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFMK------------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV-------ATA----KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISML-GKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + + + PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A+A KV Q FGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLVD IFSFYL+RD + +PGGE++ GG+D YY+G +Y VT K YWQ + + + G T C C AI DTGTSL+VGP + ++ K G ++ VS LP I +++ G+ + L P DY LK+ T+C+SGF + SG P +ILGDVFIGRYYT FD N+R+GFA
Sbjct: 22 VRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQ------KAIGAVPLIQGEVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSG-PLWILGDVFIGRYYTVFDRDNNRVGFA 400
BLAST of Lysosomal aspartic protease vs. Ensembl Human
Match: CTSD (cathepsin D [Source:HGNC Symbol;Acc:HGNC:2529]) HSP 1 Score: 360.918 bits (925), Expect = 1.930e-121 Identity = 197/386 (51.04%), Postives = 247/386 (63.99%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFMK------------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV-------ATA----KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + + + PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A+A KV Q FGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLVD IFSFYL+RD + +PGGE++ GG+D YY+G +Y VT K YW ++ S G T C C AI DTGTSL+VGP + ++ I G +I C VS LP I +++ G+ + L P DY LK+ T+C+SGF + SG P +ILGDVFIGRYYT FD N+R+GFA
Sbjct: 22 VRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYW---VEVAS--GLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSG-PLWILGDVFIGRYYTVFDRDNNRVGFA 401
BLAST of Lysosomal aspartic protease vs. Ensembl Human
Match: CTSD (cathepsin D [Source:HGNC Symbol;Acc:HGNC:2529]) HSP 1 Score: 360.147 bits (923), Expect = 4.859e-121 Identity = 195/387 (50.39%), Postives = 246/387 (63.57%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFMK------------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV-------ATA----KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISML-GKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + + + PIPE L NYMD Y+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A+A KV Q FGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLVD IFSFYL+RD + +PGGE++ GG+D YY+G +Y VT K YWQ + + + G T C C AI DTGTSL+VGP + ++ I G +I C VS LP I +++ G+ + L P DY LK+ T+C+SGF + SG P +ILGDVFIGRYYT FD N+R+GFA
Sbjct: 22 VRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMD--YYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSG-PLWILGDVFIGRYYTVFDRDNNRVGFA 405
BLAST of Lysosomal aspartic protease vs. Ensembl Celegans
Match: asp-4 (ASpartyl Protease [Source:UniProtKB/TrEMBL;Acc:Q21966]) HSP 1 Score: 341.658 bits (875), Expect = 9.220e-114 Identity = 179/350 (51.14%), Postives = 226/350 (64.57%), Query Frame = 2
Query: 221 NMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK--INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLSGIP-----FFILGDVFIGRYYTEFDLGNSRIGFAPVKT 1237
N + + ++ I E L NYMDAQYFG I+IGTP Q FTV+FDTGSSNLW+PSKKC ++AC+ H +YDS SSTY ++G +I+YGTGS+ GF+S D+V VA Q F EA EPG TFV AKFDGILGMAYP I+V V PVF + QK V + +FSF+LNR+ + + GGEI FGG D Y P TY PVT K YWQF M + G C+N C AIADTGTSLI GP++ I+ I + F G + I G +I C V LP ++ I G+ F LK DY+LK+ T+C+SGF + +P +ILGDVFIGRYY+ FD +R+GFA KT
Sbjct: 67 NHHFDKYQALNVEGEIDELLRNYMDAQYFGTISIGTPAQNFTVIFDTGSSNLWIPSKKCPFYDIACMLHHRYDSKSSSTYKEDGRKMAIQYGTGSMKGFISKDSVCVAGVCAEDQPFAEATSEPGITFVAAKFDGILGMAYPEIAVLGVQPVFNTLFEQKKVPSNLFSFWLNRNPDSEIGGEITFGGIDSRRYVEPITYVPVTRKGYWQFKMDKVVGSGVLGCSNGCQAIADTGTSLIAGPKAQIEAIQN-FIGAEPLIKGEYMISCDKVPTLPPVSFVIGGQEFSLKGEDYVLKVSQGGKTICLSGFMGID-LPERVGELWILGDVFIGRYYSVFDFDQNRVGFAQAKT 414
BLAST of Lysosomal aspartic protease vs. Ensembl Celegans
Match: asp-3 (Aspartic protease 3 [Source:UniProtKB/Swiss-Prot;Acc:P55956]) HSP 1 Score: 274.248 bits (700), Expect = 4.545e-88 Identity = 152/380 (40.00%), Postives = 212/380 (55.79%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIP------EPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVV----SQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVT-PVFQNMVAQKLVDAPIFSFYLNRDVNG-KPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD----TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKT 1237
+ R+ L+K R KF + H + IP E L +Y +AQY+G +TIGTPPQ F VLFDTGSSNLWVP C ++AC H ++D KSS+ G +F I+YGTGS+ G + D V +Q A EPG TFV AKFDGI GM + +ISV+ ++ P+ Q + +F+F+L+RD N GGEI +DPN+Y G + P+ S+ YW+ + S+ + G TY + +I DTGTSL+ GP +I I K G + NG + CS + LP I + G+NF L+ DYIL++ + + C+SGF + P +ILGDVFIGR+Y+ FD GN R+GFA +T
Sbjct: 18 IQRIKLEKRTYTREQ-YKFGSIQEHLKAKYVPGYIPNKDAFNEGLSDYSNAQYYGPVTIGTPPQNFQVLFDTGSSNLWVPCANCPFGDIACRMHNRFDCKKSSSCTATGASFEIQYGTGSMKGTVDNDVVCFGHDTTYCTDKNQGLACATSEPGITFVAAKFDGIFGMGWDTISVNKISQPMDQIFANSAICKNQLFAFWLSRDANDITNGGEITLCDTDPNHYVGNIAWEPLVSEDYWRIKLASVVIDGTTYTSGPIDSIVDTGTSLLTGPTDVIKKIQHKIGGIPLFNGEYEVECSKIPSLPNITFNLGGQNFDLQGKDYILQMSNGNGGSTCLSGFMGMDIPAPAGPLWILGDVFIGRFYSVFDHGNKRVGFATSRT 396
BLAST of Lysosomal aspartic protease vs. Ensembl Celegans
Match: asp-1 (ASpartyl Protease; Aspartic protease 1 [Source:UniProtKB/TrEMBL;Acc:G5EEI4]) HSP 1 Score: 231.876 bits (590), Expect = 9.235e-72 Identity = 138/392 (35.20%), Postives = 214/392 (54.59%), Query Frame = 2
Query: 116 IKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFN-----MMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLAC-------LTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDV---NGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDI--NSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQ---DTVCVSGFTSLSG--IPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
++ ++ ++ +++VP K ES R+ ++K + H +++ N +P +Y D Y G IT+GTPPQP TV+ DTGSSNLWV CK AC T +K+D++KS+T+VK FSI+YG+GS +G+L D + V SQ FG + F DGILG+ +P+++VD V P QN++AQK +DAP+F+ +L+R++ G PGG I +G D TY P+++KTYWQF + + ++ + I+DTGTS + P +I+ I +K + + CS++ P + I G FP+K +Y+L LQ ++ F+ SG P +ILGD FI +Y +D+GN +IGFA
Sbjct: 1 MQTFVLLALVAACSAAVIQVPTHKTESLRAKLIKEGKYTAFLASQHAARAQQLNTGFQPFVDYFDDFYLGNITLGTPPQPATVVLDTGSSNLWVIDAACK--TQACNGYPDSGYTKQKFDTTKSTTFVKETRKFSIQYGSGSCNGYLGKDVLNFGGLTVQSQEFGVS-THLADVFGYQPVDGILGLGWPALAVDQVVPPMQNLIAQKQLDAPLFTVWLDRNLQIAQGTPGGLITYGAIDTVNCAKQVTYVPLSAKTYWQFPLDAFAVGTYSETKKDQVISDTGTSWLGAPNTIVSAIVKQTKAVFDWSTELYTVDCSTMKTQPDLIFTIGGAQFPVKSVEYVLDLQLGGGKCALAVFSMGSGGFGPSWILGDTFIRQYCNVYDIGNGQIGFA 389
BLAST of Lysosomal aspartic protease vs. Ensembl Celegans
Match: asp-1 (ASpartyl Protease; Aspartic protease 1 [Source:UniProtKB/TrEMBL;Acc:G5EEI4]) HSP 1 Score: 231.876 bits (590), Expect = 9.235e-72 Identity = 138/392 (35.20%), Postives = 214/392 (54.59%), Query Frame = 2
Query: 116 IKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFN-----MMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLAC-------LTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDV---NGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDI--NSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQ---DTVCVSGFTSLSG--IPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
++ ++ ++ +++VP K ES R+ ++K + H +++ N +P +Y D Y G IT+GTPPQP TV+ DTGSSNLWV CK AC T +K+D++KS+T+VK FSI+YG+GS +G+L D + V SQ FG + F DGILG+ +P+++VD V P QN++AQK +DAP+F+ +L+R++ G PGG I +G D TY P+++KTYWQF + + ++ + I+DTGTS + P +I+ I +K + + CS++ P + I G FP+K +Y+L LQ ++ F+ SG P +ILGD FI +Y +D+GN +IGFA
Sbjct: 1 MQTFVLLALVAACSAAVIQVPTHKTESLRAKLIKEGKYTAFLASQHAARAQQLNTGFQPFVDYFDDFYLGNITLGTPPQPATVVLDTGSSNLWVIDAACK--TQACNGYPDSGYTKQKFDTTKSTTFVKETRKFSIQYGSGSCNGYLGKDVLNFGGLTVQSQEFGVS-THLADVFGYQPVDGILGLGWPALAVDQVVPPMQNLIAQKQLDAPLFTVWLDRNLQIAQGTPGGLITYGAIDTVNCAKQVTYVPLSAKTYWQFPLDAFAVGTYSETKKDQVISDTGTSWLGAPNTIVSAIVKQTKAVFDWSTELYTVDCSTMKTQPDLIFTIGGAQFPVKSVEYVLDLQLGGGKCALAVFSMGSGGFGPSWILGDTFIRQYCNVYDIGNGQIGFA 389
BLAST of Lysosomal aspartic protease vs. Ensembl Celegans
Match: asp-17 (ASpartyl Protease [Source:UniProtKB/TrEMBL;Acc:Q8MYN5]) HSP 1 Score: 226.098 bits (575), Expect = 1.598e-69 Identity = 131/375 (34.93%), Postives = 205/375 (54.67%), Query Frame = 2
Query: 158 GGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIK--NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLAC-------LTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGE---IIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDINSKFNGT--KINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK--LQDTVCVSGFTSL--SGI-PFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+ ++P K S R+ +++ + N I +P+ +Y D Y G T+GTPPQP +++ DTGS+N+WV C N+ C T +K+D+SKSS++ + FSI+YG G SG+L TDTV + + + + F V DGI G+A+P+++VD +TP QN+++Q +D P+FS +L+R + GG I +GG D TY P+T+KTYWQF M ++ + I+DTG+S I P ++I+DI ++ + T ++N + + CS++ P + I G FP+K +YIL L + C + L SG P +ILGDVFI +Y +D+GN+RIGFA
Sbjct: 15 AAVFQLPTKSTGSLRAKLIRAGKYQEFLITQHAARLNTISQPISDYSDEVYLGNFTVGTPPQPVSLVLDTGSANMWVIDASCD--NMFCNGWIGSNYTRQKFDTSKSSSFSRENRKFSIQYGKGLCSGYLGTDTVGLGGGLTIRKQELGIANKLDVDFAVQPMDGIFGLAWPALAVDQITPPMQNLISQ--LDVPVFSVWLDRKIQASHGGSAGMITYGGIDTKNCDAGVTYVPLTAKTYWQFKMDGFAVGTYSQYGYNQVISDTGSSWISAPYAMINDIATQTHATWDEMNEIYTVKCSTMKTQPDLVFTIGGALFPVKSVEYILDIGLDEGKCALAISPLMASGFGPSWILGDVFIRQYCNIYDIGNARIGFA 385
BLAST of Lysosomal aspartic protease vs. Ensembl Fly
Match: cathD (gene:FBgn0029093 transcript:FBtr0088898) HSP 1 Score: 408.683 bits (1049), Expect = 1.139e-140 Identity = 207/367 (56.40%), Postives = 243/367 (66.21%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
GLLRVPL K +SAR + L +PEPL NYMDAQY+G I IG+PPQ F V+FDTGSSNLWVPSKKC LTN+ACL H KYD+SKS TY KNGT F+I+YG+GSLSG+LSTDTV +A + QTF EA+ EPG FV AKFDGILG+ Y SISVD V P F M Q L+ AP+FSFYLNRD GGEIIFGGSDPN+Y G FTY PVT K YWQ M + S+ C C IADTGTSLI P IN K GT I G ++ C + +LPVI + G+ F L+ DYIL++ T+C+SGF L P +ILGDVFIG+YYTEFD+GN R+GFA K
Sbjct: 26 GLLRVPLHKFQSARRHFADVGTELQQLRIRYGGGDVPEPLSNYMDAQYYGPIAIGSPPQNFRVVFDTGSSNLWVPSKKCHLTNIACLMHNKYDASKSKTYTKNGTEFAIQYGSGSLSGYLSTDTVSIAGLDIKDQTFAEALSEPGLVFVAAKFDGILGLGYNSISVDKVKPPFYAMYEQGLISAPVFSFYLNRDPASPEGGEIIFGGSDPNHYTGEFTYLPVTRKAYWQIKMDAASIGDLQLCKGGCQVIADTGTSLIAAPLEEATSINQKIGGTPIIGGQYVVSCDLIPQLPVIKFVLGGKTFELEGKDYILRVAQMGKTICLSGFMGLDIPPPNGPLWILGDVFIGKYYTEFDMGNDRVGFADAK 392
BLAST of Lysosomal aspartic protease vs. Ensembl Fly
Match: cathD (gene:FBgn0029093 transcript:FBtr0452079) HSP 1 Score: 408.683 bits (1049), Expect = 1.139e-140 Identity = 207/367 (56.40%), Postives = 243/367 (66.21%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
GLLRVPL K +SAR + L +PEPL NYMDAQY+G I IG+PPQ F V+FDTGSSNLWVPSKKC LTN+ACL H KYD+SKS TY KNGT F+I+YG+GSLSG+LSTDTV +A + QTF EA+ EPG FV AKFDGILG+ Y SISVD V P F M Q L+ AP+FSFYLNRD GGEIIFGGSDPN+Y G FTY PVT K YWQ M + S+ C C IADTGTSLI P IN K GT I G ++ C + +LPVI + G+ F L+ DYIL++ T+C+SGF L P +ILGDVFIG+YYTEFD+GN R+GFA K
Sbjct: 26 GLLRVPLHKFQSARRHFADVGTELQQLRIRYGGGDVPEPLSNYMDAQYYGPIAIGSPPQNFRVVFDTGSSNLWVPSKKCHLTNIACLMHNKYDASKSKTYTKNGTEFAIQYGSGSLSGYLSTDTVSIAGLDIKDQTFAEALSEPGLVFVAAKFDGILGLGYNSISVDKVKPPFYAMYEQGLISAPVFSFYLNRDPASPEGGEIIFGGSDPNHYTGEFTYLPVTRKAYWQIKMDAASIGDLQLCKGGCQVIADTGTSLIAAPLEEATSINQKIGGTPIIGGQYVVSCDLIPQLPVIKFVLGGKTFELEGKDYILRVAQMGKTICLSGFMGLDIPPPNGPLWILGDVFIGKYYTEFDMGNDRVGFADAK 392
BLAST of Lysosomal aspartic protease vs. Ensembl Fly
Match: cathD (gene:FBgn0029093 transcript:FBtr0452080) HSP 1 Score: 408.683 bits (1049), Expect = 1.139e-140 Identity = 207/367 (56.40%), Postives = 243/367 (66.21%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
GLLRVPL K +SAR + L +PEPL NYMDAQY+G I IG+PPQ F V+FDTGSSNLWVPSKKC LTN+ACL H KYD+SKS TY KNGT F+I+YG+GSLSG+LSTDTV +A + QTF EA+ EPG FV AKFDGILG+ Y SISVD V P F M Q L+ AP+FSFYLNRD GGEIIFGGSDPN+Y G FTY PVT K YWQ M + S+ C C IADTGTSLI P IN K GT I G ++ C + +LPVI + G+ F L+ DYIL++ T+C+SGF L P +ILGDVFIG+YYTEFD+GN R+GFA K
Sbjct: 26 GLLRVPLHKFQSARRHFADVGTELQQLRIRYGGGDVPEPLSNYMDAQYYGPIAIGSPPQNFRVVFDTGSSNLWVPSKKCHLTNIACLMHNKYDASKSKTYTKNGTEFAIQYGSGSLSGYLSTDTVSIAGLDIKDQTFAEALSEPGLVFVAAKFDGILGLGYNSISVDKVKPPFYAMYEQGLISAPVFSFYLNRDPASPEGGEIIFGGSDPNHYTGEFTYLPVTRKAYWQIKMDAASIGDLQLCKGGCQVIADTGTSLIAAPLEEATSINQKIGGTPIIGGQYVVSCDLIPQLPVIKFVLGGKTFELEGKDYILRVAQMGKTICLSGFMGLDIPPPNGPLWILGDVFIGKYYTEFDMGNDRVGFADAK 392
BLAST of Lysosomal aspartic protease vs. Ensembl Fly
Match: Bace (gene:FBgn0032049 transcript:FBtr0343427) HSP 1 Score: 358.607 bits (919), Expect = 3.253e-121 Identity = 192/360 (53.33%), Postives = 239/360 (66.39%), Query Frame = 2
Query: 164 LLRVPLKKMES---ARSNIVKFNMMLN-HFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPV 1231
L RVP+ K ++ R N++ L + +++ E L N M+ Y+G I+IGTP Q F VLFD+GSSNLWVPS CK + ACLTH +YDSS SSTYV NG +FSI+YGTGSL+G+LSTDTV V + SQTF E+ EPGT F A FDGILGMAY S++VD V P F NMV+Q LVD +FSFYL RD GGE+IFGGSD + Y G TY P++ + YWQF+M S+ G + C +C AIADTGTSLIV P + ++ N + + CSSVS LP + I G NF LKPS YI++ D C+S F + G F+ILGDVFIG+YYTEFDLGN+RIGFAPV
Sbjct: 19 LHRVPILKEQNFVKTRQNVLAEKSYLRTKYQLPSLRSVDEEQLSNSMNMAYYGAISIGTPAQSFKVLFDSGSSNLWVPSNTCK--SDACLTHNQYDSSASSTYVANGESFSIQYGTGSLTGYLSTDTVDVNGLSIQSQTFAESTNEPGTNFNDANFDGILGMAYESLAVDGVAPPFYNMVSQGLVDNSVFSFYLARDGTSTMGGELIFGGSDASLYSGALTYVPISEQGYWQFTMAGSSIDGYSLCDDCQAIADTGTSLIVAPYNAYITLSEILN---VGEDGYLDCSSVSSLPDVTFNIGGTNFVLKPSAYIIQ-SDGNCMSAFEYM-GTDFWILGDVFIGQYYTEFDLGNNRIGFAPV 371
BLAST of Lysosomal aspartic protease vs. Ensembl Fly
Match: Bace (gene:FBgn0032049 transcript:FBtr0079701) HSP 1 Score: 358.607 bits (919), Expect = 3.253e-121 Identity = 192/360 (53.33%), Postives = 239/360 (66.39%), Query Frame = 2
Query: 164 LLRVPLKKMES---ARSNIVKFNMMLN-HFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPV 1231
L RVP+ K ++ R N++ L + +++ E L N M+ Y+G I+IGTP Q F VLFD+GSSNLWVPS CK + ACLTH +YDSS SSTYV NG +FSI+YGTGSL+G+LSTDTV V + SQTF E+ EPGT F A FDGILGMAY S++VD V P F NMV+Q LVD +FSFYL RD GGE+IFGGSD + Y G TY P++ + YWQF+M S+ G + C +C AIADTGTSLIV P + ++ N + + CSSVS LP + I G NF LKPS YI++ D C+S F + G F+ILGDVFIG+YYTEFDLGN+RIGFAPV
Sbjct: 19 LHRVPILKEQNFVKTRQNVLAEKSYLRTKYQLPSLRSVDEEQLSNSMNMAYYGAISIGTPAQSFKVLFDSGSSNLWVPSNTCK--SDACLTHNQYDSSASSTYVANGESFSIQYGTGSLTGYLSTDTVDVNGLSIQSQTFAESTNEPGTNFNDANFDGILGMAYESLAVDGVAPPFYNMVSQGLVDNSVFSFYLARDGTSTMGGELIFGGSDASLYSGALTYVPISEQGYWQFTMAGSSIDGYSLCDDCQAIADTGTSLIVAPYNAYITLSEILN---VGEDGYLDCSSVSSLPDVTFNIGGTNFVLKPSAYIIQ-SDGNCMSAFEYM-GTDFWILGDVFIGQYYTEFDLGNNRIGFAPV 371
BLAST of Lysosomal aspartic protease vs. Ensembl Zebrafish
Match: ctsd (cathepsin D [Source:ZFIN;Acc:ZDB-GENE-010131-8]) HSP 1 Score: 355.525 bits (911), Expect = 1.396e-119 Identity = 184/397 (46.35%), Postives = 241/397 (60.71%), Query Frame = 2
Query: 110 MNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSE-----------IKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISM-LGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKT 1237
M I L++ ++R+PLKK + R + L + S +P PE L NY+DAQY+GEI +GTP Q FTV+FDTGSSNLWVPS C LT++ACL H KY+ KSSTYVKNGT F+I+YG+GSLSG+LS DT + V Q FGEA+ +PG F+ AKFDGILGMAYP ISVD V PVF M++QK V+ +FSFYLNR+ + +PGGE++ GG+DP YY G F Y ++ + YWQ M +S+ G + C C AI DTGTSLI GP + + + + G ++ C V LP I+ + G+ + L YILK +C+SGF L P +ILGDVFIG+YYT FD N+R+GFA K+
Sbjct: 1 MRIAFLLLVAAFFCTSDAIVRIPLKKFRTLRRTLSDSGRSLEELVSSSNSLKYNLGFPASNDPTPETLKNYLDAQYYGEIGLGTPVQTFTVVFDTGSSNLWVPSVHCSLTDIACLLHHKYNGGKSSTYVKNGTQFAIQYGSGSLSGYLSQDTCTIGDIAVEKQIFGEAIKQPGVAFIAAKFDGILGMAYPRISVDGVPPVFDMMMSQKKVEKNVFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFNYVDISRQAYWQIHMDGMSIGSGLSLCKGGCEAIVDTGTSLITGPAAEVKALQKAIGAIPLMQGEYMVDCKKVPTLPTISFSLGGKVYSLTGEQYILKESQGGHDICLSGFMGLDIPPPAGPLWILGDVFIGQYYTVFDRENNRVGFAKAKS 397
BLAST of Lysosomal aspartic protease vs. Ensembl Zebrafish
Match: ctsd (cathepsin D [Source:ZFIN;Acc:ZDB-GENE-010131-8]) HSP 1 Score: 355.525 bits (911), Expect = 1.396e-119 Identity = 184/397 (46.35%), Postives = 241/397 (60.71%), Query Frame = 2
Query: 110 MNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSE-----------IKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISM-LGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKT 1237
M I L++ ++R+PLKK + R + L + S +P PE L NY+DAQY+GEI +GTP Q FTV+FDTGSSNLWVPS C LT++ACL H KY+ KSSTYVKNGT F+I+YG+GSLSG+LS DT + V Q FGEA+ +PG F+ AKFDGILGMAYP ISVD V PVF M++QK V+ +FSFYLNR+ + +PGGE++ GG+DP YY G F Y ++ + YWQ M +S+ G + C C AI DTGTSLI GP + + + + G ++ C V LP I+ + G+ + L YILK +C+SGF L P +ILGDVFIG+YYT FD N+R+GFA K+
Sbjct: 1 MRIAFLLLVAAFFCTSDAIVRIPLKKFRTLRRTLSDSGRSLEELVSSSNSLKYNLGFPASNDPTPETLKNYLDAQYYGEIGLGTPVQTFTVVFDTGSSNLWVPSVHCSLTDIACLLHHKYNGGKSSTYVKNGTQFAIQYGSGSLSGYLSQDTCTIGDIAVEKQIFGEAIKQPGVAFIAAKFDGILGMAYPRISVDGVPPVFDMMMSQKKVEKNVFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFNYVDISRQAYWQIHMDGMSIGSGLSLCKGGCEAIVDTGTSLITGPAAEVKALQKAIGAIPLMQGEYMVDCKKVPTLPTISFSLGGKVYSLTGEQYILKESQGGHDICLSGFMGLDIPPPAGPLWILGDVFIGQYYTVFDRENNRVGFAKAKS 397
BLAST of Lysosomal aspartic protease vs. Ensembl Zebrafish
Match: ctsd (cathepsin D [Source:ZFIN;Acc:ZDB-GENE-010131-8]) HSP 1 Score: 355.525 bits (911), Expect = 1.590e-119 Identity = 183/397 (46.10%), Postives = 242/397 (60.96%), Query Frame = 2
Query: 110 MNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSE-----------IKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISM-LGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKT 1237
M I L++ + ++R+PLKK + R + L + S +P PE L NY+DAQY+GEI +GTP Q FTV+FDTGSSNLWVPS C LT++ACL H KY+ KSSTYVKNGT F+I+YG+GSLSG+LS DT + V Q FGEA+ +PG F+ AKFDGILGMAYP I+VD V PVF M++QK V+ +FSFYLNR+ + +PGGE++ GG+DP YY G F Y ++ + YWQ M +S+ G + C C AI DTGTSLI GP + + + + G ++ C V LP I+ + G+ + L YILK +C+SGF L P +ILGDVFIG+YYT FD N+R+GFA K+
Sbjct: 1 MRIAFLLLVVAFFCTSDAIVRIPLKKFRTLRRTLSDSGRSLEELVSSSNSLKYNLGFPASNDPTPETLKNYLDAQYYGEIGLGTPVQTFTVVFDTGSSNLWVPSVHCSLTDIACLLHHKYNGGKSSTYVKNGTQFAIQYGSGSLSGYLSQDTCTIGDIAVEKQIFGEAIKQPGVAFIAAKFDGILGMAYPRIAVDGVPPVFDMMMSQKKVEKNVFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFNYVDISRQAYWQIHMDGMSIGSGLSLCKGGCEAIVDTGTSLITGPAAEVKALQKAIGAIPLMQGEYMVDCKKVPTLPTISFSLGGKVYSLTGEQYILKESQGGHDICLSGFMGLDIPPPAGPLWILGDVFIGQYYTVFDRENNRVGFAKAKS 397
BLAST of Lysosomal aspartic protease vs. Ensembl Zebrafish
Match: napsa (napsin A aspartic peptidase [Source:ZFIN;Acc:ZDB-GENE-030131-8690]) HSP 1 Score: 335.109 bits (858), Expect = 2.438e-111 Identity = 178/393 (45.29%), Postives = 233/393 (59.29%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNHF-----MKSEIKN-----------PIP---------EPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYC-TNCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
++R+PL KM + R + ++ MK++ + P P E L N+MDAQY+G I+IGTPPQ F+VLFDTGSSNLWVPS C ++AC HR+Y+S KSSTYV+NGT FSI+YG GSLSGF+S DTV +A V Q F EA+ +PG F VA+FDG+LGMAYP+ISVD VTPVF +A K++ IFSFY+NRD G GGE++ GG D Y+ G Y VT K YWQ M + + T C + C AI DTGTS+I GP + + + G I C + LPV++ + G+ F L +Y++K+ VC+SGF ++ P +ILGDVFIGRYYT FD R+GFAP K
Sbjct: 20 AIIRIPLHKMRTVRRMLADNGKTIDEIKSLAKMKAKYSDGTFTNQGSVTIPAPTTTQLPPPVEKLTNFMDAQYYGMISIGTPPQDFSVLFDTGSSNLWVPSIHCAFLDIACWLHRRYNSKKSSTYVQNGTEFSIQYGRGSLSGFISQDTVNLAGLNVTGQQFAEAVKQPGIVFAVARFDGVLGMAYPAISVDRVTPVFDTAMAAKILPQNIFSFYINRDPAGDVGGELMLGGFDQQYFNGDLHYVNVTRKAYWQIKMDEVQVGSTLTLCKSGCQAIVDTGTSMITGPVQEVRALQKAIGAIPLLMGEYWIDCKKIPTLPVVSFSLGGKMFNLTGQEYVMKVSHMGMNVCLSGFMAMDIPPPAGPLWILGDVFIGRYYTVFDRDQDRVGFAPAK 412
BLAST of Lysosomal aspartic protease vs. Ensembl Zebrafish
Match: ren (renin [Source:ZFIN;Acc:ZDB-GENE-040630-3]) HSP 1 Score: 289.271 bits (739), Expect = 1.019e-93 Identity = 160/374 (42.78%), Postives = 222/374 (59.36%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNM----MLNHFM-KSEIKNPI----PEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT-YCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
L RV LKKM S R + + ++ +L+ M K + +P P PL NY+D QYFGEI+IG+P Q F V+FDTGS+NLWVPS C AC TH +YD+SKS T++ NGT FSI+Y +G++ GFLS D V V V Q F EA P F+ AKFDG+LGM YP +++D +TPVF +++Q ++ +FS Y +RD PGGE++ GG+DPNY+ GPF Y + W+ M+ +S+ +C + CTA+ DTG+S I GP S I + ++ G + C+ V LP + + G+ + L DYIL + + +C F +L P +ILG FI RYYTEFD GN+RIGFA
Sbjct: 20 ALWRVKLKKMPSIRETLKEMSVTPAQVLSEIMPKYQEPSPTNGTAPTPLINYLDTQYFGEISIGSPAQMFNVVFDTGSANLWVPSHSCSPLYTACFTHNRYDASKSLTHIFNGTGFSIQYASGNVRGFLSEDVV-VVGGIPVVQVFAEATALPAIPFIFAKFDGVLGMGYPDVAIDGITPVFDRIMSQHVLKENVFSVYYSRDPTHIPGGELVLGGTDPNYHTGPFHYINTKEQGKWEVIMKGVSVGADILFCKDGCTAVIDTGSSYITGPASSISILMKTIGAVELAEGGYTVSCNVVRLLPTVAFHLGGQEYSLTDEDYILWQSEFGEDICTVTFKALDVPPPTGPVWILGANFIARYYTEFDRGNNRIGFA 392
BLAST of Lysosomal aspartic protease vs. Ensembl Xenopus
Match: ENSXETT00000012336.1 (cathepsin D-like [Source:NCBI gene;Acc:613063]) HSP 1 Score: 371.318 bits (952), Expect = 1.597e-125 Identity = 191/378 (50.53%), Postives = 243/378 (64.29%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFMKSEIK-----------NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
L+R+PLKK S R + + + +E+ P PE L NY+DAQY+GEI +G+PPQ FTV+FDTGSSNLWVPS C + ++AC H KYDSSKSSTYVKNGTAF+I+YGTGSLSG+LS DTV + V Q FGEA+ +PG TFV AKFDGILGMAYP ISVD V PVF N++AQKLV++ IFSFYLNR+ + +PGGE++ GG+DP YY G F Y VT K YWQ M + + + T C C I DTGTSLI GP + + I G ++ C V LPVI++ + G+ + L YI+K+ T+C+SGF L+ P +ILGDVFIG+YY+ FD N +GFA K
Sbjct: 22 LIRIPLKKFPSIRHTFTEAGKDVKELLANEVPLKYSPGFPPSGEPTPEALKNYLDAQYYGEIGLGSPPQNFTVVFDTGSSNLWVPSVHCSMLDIACWMHHKYDSSKSSTYVKNGTAFAIQYGTGSLSGYLSKDTVTIGNLAVKGQIFGEAVKQPGVTFVAAKFDGILGMAYPVISVDGVPPVFDNIMAQKLVESNIFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFHYLSVTRKAYWQIHMDQLGVGDQLTLCKGGCEVIVDTGTSLITGPLEEVTALQKAIGAVPLIQGQYMVQCDKVPTLPVISLTLGGQVYTLTGEQYIMKVSQRGSTICLSGFMGLNIPPPAGPLWILGDVFIGQYYSVFDRANDCVGFAKAK 399
BLAST of Lysosomal aspartic protease vs. Ensembl Xenopus
Match: ENSXETT00000028662.1 (fatty acid-binding protein, liver [Source:Xenbase;Acc:XB-GENE-5907627]) HSP 1 Score: 351.288 bits (900), Expect = 6.268e-118 Identity = 178/326 (54.60%), Postives = 223/326 (68.40%), Query Frame = 2
Query: 287 YMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK---LQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
Y+ AQY+GEI +G+PPQ FTV+FDTGSSNLWVPS C + ++AC H KYDSSKSSTYVKNGTAF+I+YGTGSLSG+LS DTV + V Q FGEA+ +PG TFV AKFDGILGMAYP ISVD V PVF N++AQKLV++ IFSFYLNR+ + +PGGE++ GG+DP YY G F Y VT K YWQ M + + + T C C I DTGTSLI GP + + I G ++ C V LPVI++ + G+ + L YI+K L T+C+SGF L+ P +ILGDVFIG+YY+ FD N+R+GFA K
Sbjct: 60 YLQAQYYGEIGLGSPPQNFTVVFDTGSSNLWVPSVHCSMLDIACWMHHKYDSSKSSTYVKNGTAFAIQYGTGSLSGYLSKDTVTIGILAVKGQIFGEAVKQPGVTFVAAKFDGILGMAYPVISVDGVPPVFDNIMAQKLVESNIFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFHYLNVTRKAYWQIHMDQLGVGDQLTLCKGGCEVIVDTGTSLITGPLEEVTALQKAIGAVPLIQGQYMVQCDKVPTLPVISLTLGGQVYTLTGEQYIMKVSQLGSTICLSGFMGLNIPPPAGPLWILGDVFIGQYYSVFDRANNRVGFAKAK 385
BLAST of Lysosomal aspartic protease vs. Ensembl Xenopus
Match: ctsd (cathepsin D [Source:NCBI gene;Acc:394561]) HSP 1 Score: 348.977 bits (894), Expect = 7.459e-117 Identity = 193/377 (51.19%), Postives = 241/377 (63.93%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFMKSEI----------KNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
L+R+PLKK S R + + + +E KNP PE L NY+DAQY+GEI IGTPPQPFTV+FDTGSSNLWVPS C +LAC H KYDSSKS+TY+ NGT F+I+YG+GSL+G+LS DTV + V Q F EA+ +PG TFV AKFDGILGM YP ISVD V PVF +++ QKLVD+ IFSFYLNR+ + PGGE++ GG+DP +Y G F Y VT K YWQ M +S+ + + C + C AI DTGTSLI GP + + I G +ILC S+ LPVI+ GR + L Y+LK+ TVC+SGF L P +I+GDVFIG+YYT FD N R+GFA K
Sbjct: 22 LVRIPLKKFTSIRRAMSETDQDALKLSGNEAATKYSAFLNSKNPTPETLLNYLDAQYYGEIGIGTPPQPFTVVFDTGSSNLWVPSIHCSFWDLACWLHHKYDSSKSTTYINNGTEFAIQYGSGSLTGYLSKDTVTIGDLAVNGQFFAEAIKQPGITFVAAKFDGILGMGYPKISVDGVPPVFDDIMEQKLVDSNIFSFYLNRNPDTLPGGELLLGGTDPAFYTGDFNYMNVTRKAYWQIHMDQLSVGDRLSLCKDGCEAIVDTGTSLITGPVEEVTALQRAIGAIPLIRGEYMILCDSIPSLPVISFTFGGRAYSLTGEQYVLKISKAGRTVCLSGFLGLDIPPPAGPLWIIGDVFIGQYYTVFDRANDRVGFAKAK 398
BLAST of Lysosomal aspartic protease vs. Ensembl Xenopus
Match: ENSXETT00000013456.1 (cathepsin D-like [Source:NCBI gene;Acc:101733630]) HSP 1 Score: 347.051 bits (889), Expect = 1.499e-116 Identity = 176/326 (53.99%), Postives = 221/326 (67.79%), Query Frame = 2
Query: 287 YMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
Y+ AQY+GEI +G+PPQ FTV+FDTGSSNLWVPS C + ++AC H KYDSSKSSTYVKNGTAF+I+YGTGSLSG+LS DTV + V Q FGEA+ +PG TFV AKFDGILGMAYP ISVD V PVF N++AQKLV++ IFSFYLNR+ + +PGGE++ GG+DP YY G F Y VT K YWQ M + + + T C C I DTGTSLI GP + + I G ++ C V LPVI++ + G+ + L YI+K+ T+C+SGF L+ P +ILGDVFIG+YY+ FD R+GFA K
Sbjct: 41 YLQAQYYGEIGLGSPPQNFTVVFDTGSSNLWVPSVHCSMLDIACWMHHKYDSSKSSTYVKNGTAFAIQYGTGSLSGYLSKDTVTIGNLAVKRQMFGEAVKQPGVTFVAAKFDGILGMAYPVISVDGVPPVFDNIMAQKLVESNIFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFHYLNVTRKAYWQIHMDQLGVGDQLTLCKGGCEVIVDTGTSLITGPLEEVTALQKAIGAVPLIQGQYMVQCDKVPTLPVISLTLGGQVYTLTGEQYIMKVSQRGSTICLSGFMGLNIPPPAGPLWILGDVFIGQYYSVFDRAYDRVGFAKAK 366
BLAST of Lysosomal aspartic protease vs. Ensembl Xenopus
Match: ctsd (cathepsin D [Source:NCBI gene;Acc:394561]) HSP 1 Score: 346.665 bits (888), Expect = 3.635e-116 Identity = 194/383 (50.65%), Postives = 241/383 (62.92%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNI----------------VKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
L ++PLKK S R + K++ LN KNP PE L NY+DAQY+GEI IGTPPQPFTV+FDTGSSNLWVPS C +LAC H KYDSSKS+TY+ NGT F+I+YG+GSL+G+LS DTV + V Q F EA+ +PG TFV AKFDGILGM YP ISVD V PVF +++ QKLVD+ IFSFYLNR+ + PGGE++ GG+DP +Y G F Y VT K YWQ M +S+ + + C + C AI DTGTSLI GP + + I G +ILC S+ LPVI+ GR + L Y+LK+ TVC+SGF L P +I+GDVFIG+YYT FD N R+GFA K
Sbjct: 7 LFKIPLKKFTSIRRAMSETDQDALKLSGNEAATKYSAFLNS------KNPTPETLLNYLDAQYYGEIGIGTPPQPFTVVFDTGSSNLWVPSIHCSFWDLACWLHHKYDSSKSTTYINNGTEFAIQYGSGSLTGYLSKDTVTIGDLAVNGQFFAEAIKQPGITFVAAKFDGILGMGYPKISVDGVPPVFDDIMEQKLVDSNIFSFYLNRNPDTLPGGELLLGGTDPAFYTGDFNYMNVTRKAYWQIHMDQLSVGDRLSLCKDGCEAIVDTGTSLITGPVEEVTALQRAIGAIPLIRGEYMILCDSIPSLPVISFTFGGRAYSLTGEQYVLKISKAGRTVCLSGFLGLDIPPPAGPLWIIGDVFIGQYYTVFDRANDRVGFAKAK 383
BLAST of Lysosomal aspartic protease vs. Ensembl Mouse
Match: Ctsd (cathepsin D [Source:MGI Symbol;Acc:MGI:88562]) HSP 1 Score: 363.614 bits (932), Expect = 1.415e-122 Identity = 194/386 (50.26%), Postives = 248/386 (64.25%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKF-----NMMLN-----HFMKSEIK--NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATA---------KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
++R+PL+K S R + + +++L + M+S K P+ E L NY+DAQY+G+I IGTPPQ FTV+FDTGSSNLWVPS CK+ ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V KV Q FGEA +PG FV AKFDGILGM YP ISV++V PVF N++ QKLVD IFSFYLNRD G+PGGE++ GG+D YY G +Y VT K YWQ M + + + T C C AI DTGTSL+VGP + ++ I G +I C VS LP + +++ G+N+ L P YILK+ T+C+SGF + SG P +ILGDVFIG YYT FD N+R+GFA
Sbjct: 21 IIRIPLRKFTSIRRTMTEVGGSVEDLILKGPITKYSMQSSPKTTEPVSELLKNYLDAQYYGDIGIGTPPQCFTVVFDTGSSNLWVPSIHCKILDIACWVHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSDQSKARGIKVEKQIFGEATKQPGIVFVAAKFDGILGMGYPHISVNNVLPVFDNLMQQKLVDKNIFSFYLNRDPEGQPGGELMLGGTDSKYYHGELSYLNVTRKAYWQVHMDQLEVGNELTLCKGGCEAIVDTGTSLLVGPVEEVKELQKAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSG-PLWILGDVFIGSYYTVFDRDNNRVGFA 405
BLAST of Lysosomal aspartic protease vs. Ensembl Mouse
Match: Ctsd (cathepsin D [Source:MGI Symbol;Acc:MGI:88562]) HSP 1 Score: 355.14 bits (910), Expect = 2.829e-119 Identity = 191/385 (49.61%), Postives = 245/385 (63.64%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKF-----NMMLN-----HFMKSEIK--NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATA---------KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
++R+PL+K S R + + +++L + M+S K P+ E L NY+DAQY+G+I IGTPPQ FTV+FDTGSSNLWVPS CK+ ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V KV Q FGEA +PG FV AKFDGILGM YP ISV++V PVF N++ QKLVD IFSFYLNRD G+PGGE++ GG+D YY G +Y VT K YW +++ C C AI DTGTSL+VGP + ++ I G +I C VS LP + +++ G+N+ L P YILK+ T+C+SGF + SG P +ILGDVFIG YYT FD N+R+GFA
Sbjct: 21 IIRIPLRKFTSIRRTMTEVGGSVEDLILKGPITKYSMQSSPKTTEPVSELLKNYLDAQYYGDIGIGTPPQCFTVVFDTGSSNLWVPSIHCKILDIACWVHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSDQSKARGIKVEKQIFGEATKQPGIVFVAAKFDGILGMGYPHISVNNVLPVFDNLMQQKLVDKNIFSFYLNRDPEGQPGGELMLGGTDSKYYHGELSYLNVTRKAYWLEVGNELTL-----CKGGCEAIVDTGTSLLVGPVEEVKELQKAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPDKYILKVSQGGKTICLSGFMGMDIPPPSG-PLWILGDVFIGSYYTVFDRDNNRVGFA 399
BLAST of Lysosomal aspartic protease vs. Ensembl Mouse
Match: Ctse (cathepsin E [Source:MGI Symbol;Acc:MGI:107361]) HSP 1 Score: 307.375 bits (786), Expect = 9.047e-101 Identity = 170/382 (44.50%), Postives = 229/382 (59.95%), Query Frame = 2
Query: 158 GGLLRVPLKKMESARSNIVKFNMMLNHFMKSE------------IKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT--YCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTV-----CVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPV 1231
G L RVPL++ +S R + + L+ F +S + + + EPL NY+D +YFG I+IGTPPQ FTV+FDTGSSNLWVPS C T+ AC H + S+S TY + G FSI+YGTGSL+G + D V V V Q FGE++ EPG TFV A+FDGILG+ YPS++ VTPVF NM+AQ LV P+FS YL+ D G G E+ FGG DP+++ G + PVT + YWQ ++ I +G T +C+ C AI DTGTSLI GP I + T I+G + C+++ +P + I ++ L P+DYIL D V C SGF L P +ILGDVFI ++Y+ FD GN+++G AP
Sbjct: 20 GALHRVPLRRHQSLRKKL-RAQGQLSEFWRSHNLDMTRLSESCNVYSSVNEPLINYLDMEYFGTISIGTPPQNFTVIFDTGSSNLWVPSVYC--TSPACKAHPVFHPSQSDTYTEVGNHFSIQYGTGSLTGIIGADQVSVEGLTVDGQQFGESVKEPGQTFVNAEFDGILGLGYPSLAAGGVTPVFDNMMAQNLVALPMFSVYLSSDPQGGSGSELTFGGYDPSHFSGSLNWIPVTKQAYWQIALDGI-QVGDTVMFCSEGCQAIVDTGTSLITGPPDKIKQLQEAIGATPIDGEYAVDCATLDTMPNVTFLINEVSYTLNPTDYILP--DLVEGMQFCGSGFQGLDIPPPAGPLWILGDVFIRQFYSVFDRGNNQVGLAPA 395
BLAST of Lysosomal aspartic protease vs. Ensembl Mouse
Match: Pgc (progastricsin (pepsinogen C) [Source:MGI Symbol;Acc:MGI:98909]) HSP 1 Score: 302.368 bits (773), Expect = 7.910e-99 Identity = 169/396 (42.68%), Postives = 234/396 (59.09%), Query Frame = 2
Query: 116 IKLLIIALVINGIF-GGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNP--------------IPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQS--ISMLGKTYCTN--CTAIADTGTSLIVGPQSIIDDINSKFNGTKIN-GMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFAP 1228
+K +++AL+ + L+RVPLKKM+S R + K +L F+K+ +P + EP+ YMDA Y+GEI+IGTPPQ F VLFDTGSSNLWV S C+ + AC TH +Y+ SKSSTY G FS++YGTGSL+GF DT+ V + +V +Q FG + EPGT FV A+FDGI+G+AYP +S T Q M+ + + P+F YL G GG+I+FGG D N Y G T+ PVT + YWQ ++ I +C++ C I DTGTSL+V P ++++ + G + C SVS LP + + G FPL PS YI++ ++ C+ G SL SG P +ILGDVF+ YY FD+GN+R+G AP
Sbjct: 1 MKWMVVALLCLPLLEAALIRVPLKKMKSIRETM-KEQGVLKDFLKNHKYDPGQKYHFGKFGDYSVLYEPM-AYMDASYYGEISIGTPPQNFLVLFDTGSSNLWVSSVYCQ--SEACTTHTRYNPSKSSTYYTQGQTFSLQYGTGSLTGFFGYDTLRVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPGLSSGGATTALQGMLGEGALSQPLFGVYLGSQ-QGSNGGQIVFGGVDENLYTGELTWIPVTQELYWQITIDDFLIGNQASGWCSSSGCQGIVDTGTSLLVMPAQYLNELLQTIGAQEGEYGQYFVSCDSVSSLPTLTFVLNGVQFPLSPSSYIIQ-EEGSCMVGLESLSLNAESGQPLWILGDVFLRSYYAVFDMGNNRVGLAP 390
BLAST of Lysosomal aspartic protease vs. Ensembl Mouse
Match: Napsa (napsin A aspartic peptidase [Source:MGI Symbol;Acc:MGI:109365]) HSP 1 Score: 301.982 bits (772), Expect = 2.893e-98 Identity = 165/382 (43.19%), Postives = 221/382 (57.85%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFN--MMLNHFMKSEIK--NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISM-LGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK-LQDTV--CVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNS----RIGFAPVKTPSKDET 1255
L+RVPL+++ + N L ++ NP PL +M+ QYFG I +GTPPQ FTV+FDTGSSNLWVPS +C +LAC H +++ SS++ NGT F+I+YGTG LSG LS D + + TFGEA+ EP F +A FDGILG+ +P+++V V P MV Q L++ P+FSFYLNRD G GGE++ GGSDP +Y P T+ PVT YWQ M+S+ + G + C C+AI DTGTSLI GP I +N G +NG I CS LP ++ + G F L DY++K LQ V C+ GF +L P +ILGDVF+G Y FD G+ R+G A ++ S D
Sbjct: 23 LIRVPLQRIHLGHRILNPLNGWEQLAELSRTSTSGGNPSFVPLSKFMNTQYFGTIGLGTPPQNFTVVFDTGSSNLWVPSTRCHFFSLACWFHHRFNPKASSSFRPNGTKFAIQYGTGRLSGILSQDNLTIGGIHDAFVTFGEALWEPSLIFALAHFDGILGLGFPTLAVGGVQPPLDAMVEQGLLEKPVFSFYLNRDSEGSDGGELVLGGSDPAHYVPPLTFIPVTIPAYWQVHMESVKVGTGLSLCAQGCSAILDTGTSLITGPSEEIRALNKAIGGYPFLNGQYFIQCSKTPTLPPVSFHLGGVWFNLTGQDYVIKILQSDVGLCLLGFQALDIPKPAGPLWILGDVFLGPYVAVFDRGDKNVGPRVGLARAQSRSTDRA 404
BLAST of Lysosomal aspartic protease vs. UniProt/SwissProt
Match: sp|Q03168|ASPP_AEDAE (Lysosomal aspartic protease OS=Aedes aegypti OX=7159 GN=AAEL006169 PE=1 SV=2) HSP 1 Score: 409.838 bits (1052), Expect = 3.348e-140 Identity = 210/379 (55.41%), Postives = 258/379 (68.07%), Query Frame = 2
Query: 128 IIALVINGIFG--GLLRVPLKKMESARSNIVKFNMMLNHFM--KSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
IIALV + +RV L K ESAR + + + + + P+PEPL NY+DAQY+G ITIGTPPQ F V+FDTGSSNLWVPSK+C TN+ACL H KY++ KSST+ KNGTAF I+YG+GSLSG+LSTDTV + V QTF EA+ EPG FV AKFDGILG+ Y SISVD V PVF NM Q L+DAP+FSFYLNRD + GGEIIFGGSD N Y G FTY V K YWQF M S+ + +C N C AIADTGTSLI GP S + IN GT I NG ++ CS + KLP I+ + G++F L+ +DY+L++ T+C+SGF + P +ILGDVFIG+YYTEFD+GN R+GFA
Sbjct: 6 IIALVCLAVLAQADFVRVQLHKTESARQHFRNVDTEIKQLRLKYNAVSGPVPEPLSNYLDAQYYGAITIGTPPQSFKVVFDTGSSNLWVPSKECSFTNIACLMHNKYNAKKSSTFEKNGTAFHIQYGSGSLSGYLSTDTVGLGGVSVTKQTFAEAINEPGLVFVAAKFDGILGLGYSSISVDGVVPVFYNMFNQGLIDAPVFSFYLNRDPSAAEGGEIIFGGSDSNKYTGDFTYLSVDRKAYWQFKMDSVKVGDTEFCNNGCEAIADTGTSLIAGPVSEVTAINKAIGGTPIMNGEYMVDCSLIPKLPKISFVLGGKSFDLEGADYVLRVAQMGKTICLSGFMGIDIPPPNGPLWILGDVFIGKYYTEFDMGNDRVGFA 384
BLAST of Lysosomal aspartic protease vs. UniProt/SwissProt
Match: sp|Q05744|CATD_CHICK (Cathepsin D OS=Gallus gallus OX=9031 GN=CTSD PE=1 SV=1) HSP 1 Score: 381.719 bits (979), Expect = 6.244e-129 Identity = 197/381 (51.71%), Postives = 249/381 (65.35%), Query Frame = 2
Query: 149 GIFGGLLRVPLKKMESAR-------SNIVKFNMMLNHFMK-----SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISML-GKTYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQ---DTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
G L+R+PL K S R S I N + F+K +++ P PE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS C L ++ACL H KYD+SKSSTYV+NGT F+I YGTGSLSGFLS DTV + K+ +Q FGEA+ +PG TF+ AKFDGILGMA+P ISVD VTP F N++ QKL++ IFSFYLNRD +PGGE++ GG+DP YY G F++ VT K YWQ M S+ + G T C C AI DTGTSLI GP + ++ + I G +I C +S LPV+ + + G+ + L Y+ K+ +T+C+SGF+ L G P +ILGDVFIG YYT FD N +GFA
Sbjct: 16 GPCAALIRIPLTKFTSTRRMLTEVGSEIPDMNA-ITQFLKFKLGFADLAEPTPEILKNYMDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSVHCHLLDIACLLHHKYDASKSSTYVENGTEFAIHYGTGSLSGFLSQDTVTLGNLKIKNQIFGEAVKQPGITFIAAKFDGILGMAFPRISVDKVTPFFDNVMQQKLIEKNIFSFYLNRDPTAQPGGELLLGGTDPKYYSGDFSWVNVTRKAYWQVHMDSVDVANGLTLCKGGCEAIVDTGTSLITGPTKEVKELQTAIGAKPLIKGQYVISCDKISSLPVVTLMLGGKPYQLTGEQYVFKVSAQGETICLSGFSGLDVPPPGGPLWILGDVFIGPYYTVFDRDNDSVGFA 395
BLAST of Lysosomal aspartic protease vs. UniProt/SwissProt
Match: sp|P07339|CATD_HUMAN (Cathepsin D OS=Homo sapiens OX=9606 GN=CTSD PE=1 SV=1) HSP 1 Score: 369.392 bits (947), Expect = 6.918e-124 Identity = 197/387 (50.90%), Postives = 248/387 (64.08%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFMK------------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV-------ATA----KVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISML-GKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + + + PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A+A KV Q FGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLVD IFSFYL+RD + +PGGE++ GG+D YY+G +Y VT K YWQ + + + G T C C AI DTGTSL+VGP + ++ I G +I C VS LP I +++ G+ + L P DY LK+ T+C+SGF + SG P +ILGDVFIGRYYT FD N+R+GFA
Sbjct: 22 VRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSG-PLWILGDVFIGRYYTVFDRDNNRVGFA 407
BLAST of Lysosomal aspartic protease vs. UniProt/SwissProt
Match: sp|Q4LAL9|CATD_CANLF (Cathepsin D OS=Canis lupus familiaris OX=9615 GN=CTSD PE=2 SV=1) HSP 1 Score: 365.925 bits (938), Expect = 1.257e-122 Identity = 196/384 (51.04%), Postives = 243/384 (63.28%), Query Frame = 2
Query: 167 LRVPLKKMESARSNIVKFNMMLNHFM-KSEIKN-----------PIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV---------ATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
+R+PL K S R + + + + K I PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGSSNLWVPS CKL ++AC H KY+S KSSTYVKNGT+F I YG+GSLSG+LS DTV V A KV QTFGEA +PG TF+ AKFDGILGMAYP ISV++V PVF N++ QKLV+ IFSFYLNRD N +PGGE++ GG+D YY+GP +Y VT K YWQ M+ + + T C C AI DTGTSLIVGP + ++ I G +I C VS LP + +++ G+ + L DY LK+ T+C+SGF + G P +ILGDVFIG YYT FD +R+G A
Sbjct: 22 VRIPLHKFTSVRRTMTELGGPVEDLIAKGPISKYAQGAPAVTGGPIPEMLRNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSGKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCKSALSGLAGIKVERQTFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVEKNIFSFYLNRDPNAQPGGELMLGGTDSKYYKGPLSYLNVTRKAYWQVHMEQVDVGSSLTLCKGGCEAIVDTGTSLIVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPDVTLKLGGKLYKLSSEDYTLKVSQGGKTICLSGFMGMDIPPPGGPLWILGDVFIGCYYTVFDRDQNRVGLA 405
BLAST of Lysosomal aspartic protease vs. UniProt/SwissProt
Match: sp|P80209|CATD_BOVIN (Cathepsin D OS=Bos taurus OX=9913 GN=CTSD PE=1 SV=2) HSP 1 Score: 364.77 bits (935), Expect = 1.731e-122 Identity = 192/385 (49.87%), Postives = 244/385 (63.38%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFM-KSEI-----------KNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIV---------ATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKL---QDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
++R+PL K S R + + + + K I + PIPE L NYMDAQY+GEI IGTPPQ FTV+FDTGS+NLWVPS CKL ++AC THRKY+S KSSTYVKNGT F I YG+GSLSG+LS DTV V V QTFGEA+ +PG F+ AKFDGILGMAYP ISV++V PVF N++ QKLVD +FSF+LNRD +PGGE++ GG+D YY+G + VT + YWQ M + + T C C AI DTGTSLIVGP + ++ I G +I C VS LP + +++ G+++ L P DY LK+ + TVC+SGF + G P +ILGDVFIGRYYT FD +R+G A
Sbjct: 1 VIRIPLHKFTSIRRTMSEAAGXVXXLIAKGPISKYATGEPAVRQGPIPELLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSANLWVPSIHCKLLDIACWTHRKYNSDKSSTYVKNGTTFDIHYGSGSLSGYLSQDTVSVPCNPSSSSPGGVTVQRQTFGEAIKQPGVVFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDKNVFSFFLNRDPKAQPGGELMLGGTDSKYYRGSLMFHNVTRQAYWQIHMDQLDVGSSLTVCKGGCEAIVDTGTSLIVGPVEEVRELQKAIGAVPLIQGEYMIPCEKVSSLPEVTVKLGGKDYALSPEDYALKVSQAETTVCLSGFMGMDIPPPGGPLWILGDVFIGRYYTVFDRDQNRVGLA 385
BLAST of Lysosomal aspartic protease vs. TrEMBL
Match: A0A2L2Y403 (Lysosomal aspartic protease (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1) HSP 1 Score: 426.787 bits (1096), Expect = 2.063e-144 Identity = 221/398 (55.53%), Postives = 271/398 (68.09%), Query Frame = 2
Query: 74 FRIYILHNFINMMNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFN---MMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT--YCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK---LQDTVCVSGFTSLSGI----PFFILGDVFIGRYYTEFDLGNSRIGFA 1225
F I+IL NF + L ++PL +M+S R ++ + ++N P PEPL NY+DAQY+G I+IGTPPQPF V+FDTGSSNLWVPSKKCK TN+ACL H KYDSS+SSTY +NGT IRYG+GS++GFLSTD V V +K+ QTFGEA+ EPG FV AKFDGILG+ + SI+VD V PVF NMV QKLVD+PIFSFYLNRD + GGEIIFGGSDP++Y+G FTY PV + YWQF M +S+ GKT YC C AIADTGTSLI GP + ++ +N + T + G +I C + KLP I++ GRNF L+ SDY+LK L TVC+SGF L P +ILGDVFIGRYYTEFD GNSR+GFA
Sbjct: 4 FSIFILLNFASCTR----------------LFKIPLLRMQSIREHMHEVGTPIHIVNQKWSHLEAGPFPEPLSNYLDAQYYGSISIGTPPQPFKVVFDTGSSNLWVPSKKCKWTNIACLLHNKYDSSQSSTYKENGTELEIRYGSGSMTGFLSTDIVTVGGSKIQDQTFGEAITEPGLAFVAAKFDGILGLGFSSIAVDGVVPVFDNMVKQKLVDSPIFSFYLNRDPKAEVGGEIIFGGSDPDHYKGNFTYVPVDRRGYWQFPMDGLSINGKTGVYCACGCEAIADTGTSLIAGPVAEVEKLNKELGATPLVQGEYMIDCDLIPKLPKISLTFGGRNFDLQGSDYVLKVSQLGKTVCLSGFIGLDVPKPMGPLWILGDVFIGRYYTEFDQGNSRVGFA 385
BLAST of Lysosomal aspartic protease vs. TrEMBL
Match: A0A232ETM9 (Peptidase A1 domain-containing protein OS=Trichomalopsis sarcophagae OX=543379 GN=TSAR_007250 PE=3 SV=1) HSP 1 Score: 426.402 bits (1095), Expect = 3.274e-144 Identity = 218/383 (56.92%), Postives = 263/383 (68.67%), Query Frame = 2
Query: 116 IKLLIIA-LVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
++L+ +A L + + +LRVPL +++SAR + + L+ +P+PEPL NY+DAQY+GEI IG+P Q FTV+FDTGSSNLWVPSKKC +TN+ACL H KYDS KS +Y NGT FSIRYG+GSLSGFLSTD V +A V TF EAM EPG FV AKFDGILGMAY ISVD V PVF NMV Q LV PIFSFYLNRD N K GGE+I GGSDP +Y G FTY PV+ K YWQF M IS+ K +C N C AIADTGTSLI GP I+ IN K T I G ++ C +V LP I+ + G+NF LK +Y+LK+ T+C+SGF + P +ILGDVFIGR+YTEFD+GN RIGFA K
Sbjct: 2 LRLVFLASLCLAFVTADVLRVPLYRVKSARRTLQEVGTELHQIKLRYGADPVPEPLSNYLDAQYYGEIGIGSPMQKFTVIFDTGSSNLWVPSKKCHITNIACLLHNKYDSRKSKSYKANGTEFSIRYGSGSLSGFLSTDVVTIAGVDVKDTTFAEAMSEPGLAFVAAKFDGILGMAYDRISVDGVPPVFYNMVKQNLVPQPIFSFYLNRDPNAKIGGEMILGGSDPAHYTGDFTYVPVSRKAYWQFKMDKISIGDKLFCENGCEAIADTGTSLIAGPVGEIEGINKKIGATPIVAGEAMVSCDAVPNLPTIDFVVGGKNFSLKGEEYVLKVAQFGKTICLSGFMGIDIPPPNGPLWILGDVFIGRFYTEFDMGNDRIGFANAK 384
BLAST of Lysosomal aspartic protease vs. TrEMBL
Match: A0A2L2Y0H5 (Lysosomal aspartic protease OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1) HSP 1 Score: 426.402 bits (1095), Expect = 3.854e-144 Identity = 214/370 (57.84%), Postives = 264/370 (71.35%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFMKSEI--KNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT--YCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
LLRV L R + + + + I K+P PEPL NY+DAQY+G+I+IGTPPQ F V+FDTGSSNLWVPSKKCK TN+ACL H KYDSSKSSTY NGTAF I+YG+GS++GFLSTDTV + +V SQTF EA+ EPG TFV AKFDGILGM + +ISVD VTPVF NM+ +K+VD P+FSFYL+R+ N GGEIIFGGSDPN+Y+G FTY PV K YWQF M SIS+ GKT YC++ C AIADTGTSLI GP ID +N + T + G ++ C+ + KLP ++ + G+ F LK SDY++ +L T+C+SGF + P +ILGDVFIGRYYTEFD NSR+GFA K
Sbjct: 21 LLRVKLAHSRPVRHKLHEVGTPVEIALPRRILGKSPFPEPLSNYLDAQYYGQISIGTPPQTFKVVFDTGSSNLWVPSKKCKWTNIACLLHNKYDSSKSSTYKANGTAFEIKYGSGSMTGFLSTDTVAIGGVQVASQTFAEAVTEPGLTFVAAKFDGILGMGFSTISVDGVTPVFDNMLKEKVVDKPVFSFYLSRNPNASAGGEIIFGGSDPNHYKGDFTYVPVDKKGYWQFHMDSISVNGKTSAYCSSGCEAIADTGTSLIAGPVDEIDKLNKEIGATPLMQGEYVVDCNLIPKLPTVSFTLGGKKFDLKGSDYVIQVSQLGQTICLSGFMGIDIPKPAGPLWILGDVFIGRYYTEFDYANSRVGFADAK 390
BLAST of Lysosomal aspartic protease vs. TrEMBL
Match: A0A2L2Y439 (Lysosomal aspartic protease (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1) HSP 1 Score: 425.631 bits (1093), Expect = 6.205e-144 Identity = 220/398 (55.28%), Postives = 271/398 (68.09%), Query Frame = 2
Query: 74 FRIYILHNFINMMNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFN---MMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT--YCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK---LQDTVCVSGFTSLSGI----PFFILGDVFIGRYYTEFDLGNSRIGFA 1225
F I+IL NF + L ++PL +M+S R ++ + ++N P PEPL NY+DAQY+G I+IGTPPQPF V+FDTGSSNLWVPSKKCK TN+ACL H KYDSS+SSTY +NGT IRYG+GS++GFLSTD V V +K+ QTFGEA+ EPG FV AKFDGILG+ + SI+VD V PVF NMV QKLVD+PIFSFYLNRD + GGEIIFGGSDP++Y+G FTY PV + YWQF M +S+ GKT YC C AIADTGTSLI GP + ++ +N + T + G +I C + KLP I++ GRNF L+ SDY+LK L +VC+SGF L P +ILGDVFIGRYYTEFD GNSR+GFA
Sbjct: 4 FSIFILLNFASCTR----------------LFKIPLLRMQSIREHMHEVGTPIHIVNQKWSHLEAGPFPEPLSNYLDAQYYGSISIGTPPQPFKVVFDTGSSNLWVPSKKCKWTNIACLLHNKYDSSQSSTYKENGTELEIRYGSGSMTGFLSTDIVTVGGSKIQDQTFGEAITEPGLAFVAAKFDGILGLGFSSIAVDGVVPVFDNMVKQKLVDSPIFSFYLNRDPKAEVGGEIIFGGSDPDHYKGNFTYVPVDRRGYWQFPMDGLSINGKTGVYCACGCEAIADTGTSLIAGPVAEVEKLNKELGATPLVQGEYMIDCDLIPKLPKISLTFGGRNFDLQGSDYVLKVSQLGKSVCLSGFIGLDVPKPMGPLWILGDVFIGRYYTEFDQGNSRVGFA 385
BLAST of Lysosomal aspartic protease vs. TrEMBL
Match: A0A482X3U9 (Peptidase A1 domain-containing protein OS=Laodelphax striatellus OX=195883 GN=LSTR_LSTR002334 PE=3 SV=1) HSP 1 Score: 423.32 bits (1087), Expect = 6.507e-143 Identity = 216/390 (55.38%), Postives = 269/390 (68.97%), Query Frame = 2
Query: 116 IKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFM--------KSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKP--GGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSL-----SGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
+K ++ L I G L+RVP+ K +S R V+F L S++K E L NY DAQYFGEI++GTPPQPF V+FDTGSSNLWVPSKKC N+ACLTH KYD++KSSTY KNG F I YGTGSLSGFLSTDT+ + K+ +QTFGEA+ EPG TFV AKFDGILG+A+ SIS D VTP F NM+ Q+L+ P+FSFYLNR+ + GGEIIFGGSDP Y+G FTY PV YWQF M + + GKT+C C AIADTGTSLIVGP + + +IN T++ G ++ CS +SKLP I+I +AG+ F L DY+LK+ + +C+SGF+ + SG P +ILGDVFIG+YYTEFD GN R+GFA VK
Sbjct: 1 MKAFVLLLGIGLCCGELMRVPVYKADSIRKQFVEFGNELPQSGIVYGLGSDGSKLKEGTAERLTNYFDAQYFGEISLGTPPQPFQVIFDTGSSNLWVPSKKCSYFNIACLTHHKYDATKSSTYSKNGKEFDIHYGTGSLSGFLSTDTLTIGDMKIKNQTFGEAVKEPGMTFVAAKFDGILGLAFSSISQDSVTPPFYNMMTQQLIKNPVFSFYLNRNASAPAGQGGEIIFGGSDPEKYEGEFTYLPVDRPAYWQFKMDKVQVSGKTFCAKGCEAIADTGTSLIVGPVAEVTEINKLIGATEMPGGQYVVECSLISKLPEISIILAGKEFKLSGEDYVLKISEKICLSGFSGMDIPPPSG-PLWILGDVFIGKYYTEFDFGNKRVGFASVK 389
BLAST of Lysosomal aspartic protease vs. Ensembl Cavefish
Match: ctsd (cathepsin D [Source:ZFIN;Acc:ZDB-GENE-010131-8]) HSP 1 Score: 354.369 bits (908), Expect = 3.212e-119 Identity = 180/376 (47.87%), Postives = 237/376 (63.03%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFMKSEI------------KNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDT---VCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
++R+PLKK S R ++ + + P PE L NY+DAQY+GEI +GTP Q FTV+FDTGSSNLWVPS C LT++ACL H KY+ +KSSTYVKNGTAF+I+YG+GSLSG+LS DT + V Q FGEA+ +PG F+ AKFDGILGMAYP ISVD V PVF M++QK V+ +FSFYLNR+ + +PGGE++ GG+DP YY G F Y ++ + YWQ M +++ + C C AI DTGTSLI GP + + + I G ++ C V LP I+ + G+++ L YILK +C+SGF +L P +ILGDVFIG+YYTEFD N+R+GFA
Sbjct: 21 IVRIPLKKFRSIRRSMTDSGRAMEELLADSQSTKYNLGFPPPSNGPTPETLKNYLDAQYYGEIGLGTPIQTFTVVFDTGSSNLWVPSVHCSLTDIACLLHHKYNGAKSSTYVKNGTAFAIQYGSGSLSGYLSQDTCTIGDIAVEKQIFGEAIKQPGVAFIAAKFDGILGMAYPRISVDGVPPVFDMMMSQKKVEKNVFSFYLNRNPDTQPGGELLLGGTDPKYYTGDFHYVNISRQAYWQVHMDGMNIGSQLNLCKGGCEAIVDTGTSLITGPAAEVKALQKAIGAIPLIQGEYMVDCKKVPSLPTISFVLGGQSYTLTGEQYILKESQAGKEICLSGFMALDIPPPAGPLWILGDVFIGQYYTEFDRENNRVGFA 396
BLAST of Lysosomal aspartic protease vs. Ensembl Cavefish
Match: ENSAMXT00000045448.1 (cathepsin D-like [Source:NCBI gene;Acc:103032210]) HSP 1 Score: 341.273 bits (874), Expect = 9.240e-114 Identity = 175/331 (52.87%), Postives = 215/331 (64.95%), Query Frame = 2
Query: 272 EPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
E L N+MD+QYFG I+IGTPPQ FTVLFDTGSSNLWVPS C +LAC H +Y+S KSSTYVKNGT+FSIRYG GSLSGF+S DTV +A V Q F EA+ +PG F VA+FDG+LGMAYP+ISVD V PVF +A KL+ IFSFY NRD + GGE++ GG D Y+ G Y VT K YWQF M + + T C C AIADTGTS+IVGP I +N + G I C + LP ++I + G+ F L DY++ ++ ++C+SGF + P +ILGDVFIGRYYT FD N R+GFAP K
Sbjct: 85 EKLTNFMDSQYFGVISIGTPPQEFTVLFDTGSSNLWVPSVHCSFFDLACWLHHRYNSKKSSTYVKNGTSFSIRYGRGSLSGFISQDTVNLAGLNVTGQQFAEAVKQPGIVFAVARFDGVLGMAYPTISVDGVRPVFDTAMASKLLPRNIFSFYFNRDTAAEIGGELMLGGFDAQYFDGDLHYVSVTRKAYWQFLMDEVQVGSTLTICKGGCQAIADTGTSMIVGPVQEIRALNKAIGAIPLLMGEYFIDCKKIPSLPTVSISLGGKMFNLTGKDYVVQETQMGVSICLSGFMGMDVPPPAGPLWILGDVFIGRYYTVFDRDNDRVGFAPTK 415
BLAST of Lysosomal aspartic protease vs. Ensembl Cavefish
Match: ren (renin [Source:NCBI gene;Acc:103036504]) HSP 1 Score: 297.745 bits (761), Expect = 4.049e-97 Identity = 163/375 (43.47%), Postives = 221/375 (58.93%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNH-FMKSEIKNPI---------PEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISM-LGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
L RV +KKM S R + + + F + E+K+ I P PL NY+DAQYFGEI+IG+P Q F V+FDTGS+NLWVPS C AC TH +YD+SKS T+V NGT FSI+Y +G++ GFLS D V V V Q F EA P F+ AKFDG+LGM YP+ ++D +TPVF +++Q ++ +FS Y +RD PGGE++ GG+DPNYY G F Y P + W+ M+ +S+ K +CT CTA+ DTG+S I GP S + + ++ G + C V LP + + G+ +PL DYIL + + +C F +L P +ILG FI RYYTEFD N+RIGFA
Sbjct: 19 ALQRVRMKKMPSIRETLKETGVTPAQVFAELELKSDISLSPRNGTAPTPLTNYLDAQYFGEISIGSPAQMFNVVFDTGSANLWVPSHMCSPLYTACFTHNRYDASKSHTHVYNGTGFSIQYASGNVRGFLSEDIV-VVGGIPVVQVFAEATALPAIPFIFAKFDGVLGMGYPNAAIDGITPVFDRIMSQHVLKEEVFSVYYSRDPQSIPGGELVLGGTDPNYYTGSFNYVPTKGEGKWEVIMKGVSVGADKLFCTEGCTAVIDTGSSFITGPASSVSILMKTIGAVELAEGGYTVNCDLVMSLPSVIFHLGGKEYPLTQEDYILWQSQFGEDICTVTFRALDVPPPTGPVWILGANFIARYYTEFDRRNNRIGFA 392
BLAST of Lysosomal aspartic protease vs. Ensembl Cavefish
Match: ren (renin [Source:NCBI gene;Acc:103036504]) HSP 1 Score: 285.419 bits (729), Expect = 2.634e-92 Identity = 162/380 (42.63%), Postives = 220/380 (57.89%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNH-FMKSEIKNPI---------PEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFY-------LNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISM-LGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
L RV +KKM S R + + + F + E+K+ I P PL NY+DAQYFGEI+IG+P Q F V+FDTGS+NLWVPS C AC TH +YD+SKS T+V NGT FSI+Y +G++ GFLS D V V V Q F EA P F+ AKFDG+LGM YP+ ++D +TPVF +++Q ++ +FS Y +N N PGGE++ GG+DPNYY G F Y P + W+ M+ +S+ K +CT CTA+ DTG+S I GP S + + + + + C V LP + + G+ +PL DYIL + + +C F +L P +ILG FI RYYTEFD N+RIGFA
Sbjct: 12 LNRVRMKKMPSIRETLKETGVTPAQVFAELELKSDISLSPRNGTAPTPLTNYLDAQYFGEISIGSPAQMFNVVFDTGSANLWVPSHMCSPLYTACFTHNRYDASKSHTHVYNGTGFSIQYASGNVRGFLSEDIV-VVGGIPVVQVFAEATALPAIPFIFAKFDGVLGMGYPNAAIDGITPVFDRIMSQHVLKEEVFSVYYSSENLIINNFKNIIPGGELVLGGTDPNYYTGSFNYVPTKGEGKWEVIMKGVSVGADKLFCTEGCTAVIDTGSSFITGPASSVSILMKTIGAVE---LYTVNCDLVMSLPSVIFHLGGKEYPLTQEDYILWQSQFGEDICTVTFRALDVPPPTGPVWILGANFIARYYTEFDRRNNRIGFA 387
BLAST of Lysosomal aspartic protease vs. Ensembl Cavefish
Match: nots (cathepsin E-like [Source:NCBI gene;Acc:103036771]) HSP 1 Score: 284.648 bits (727), Expect = 6.787e-92 Identity = 156/392 (39.80%), Postives = 229/392 (58.42%), Query Frame = 2
Query: 164 LLRVPLKKMESARSNIVKFNMMLNHFMKSEIK-------------------NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT-YC----TNCTAIADTGTSLIVGPQSIIDDINSKFNGTKI-NGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK--LQDTV-CVSGFTSLSGI----PFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPS 1243
L+R+PL K S RS++ N + + ++ E L N+MDAQYFG+I++GTP Q FTV+FDTGS++LWVPS C + AC +H K+ + +SSTY+ +G F I YG+G L G L+ D + V + V +Q FGE++ EPG +FV+ KFDGILG++YP+++ +H PVF NM+ Q LV+ PIFSF+LN + + GE++ GG D + + P + PVT K YWQ M + + G + +C ++C AI DTGTSLI GP I + T G ++ CS + LPVI+ + G + L YI K + D V C++GF ++ + P +ILGDVF+ + Y+ +D G R+GFA +K PS
Sbjct: 18 LVRIPLTKAPSLRSHLRAKNQLEDFLKDHQVDAFSRRYAQCYPSALLSLKSGKTAERLYNFMDAQYFGQISLGTPAQNFTVVFDTGSADLWVPSTYC--VSPACASHHKFKAIESSTYIHDGRIFGIHYGSGHLLGVLAKDMLKVGSVTVQNQEFGESVYEPGMSFVMTKFDGILGLSYPALAEEHGAPVFDNMIKQNLVEKPIFSFFLN--IGPEKEGELLLGGVDEDLFVPPINWVPVTVKGYWQIKMDVLKVQGTSAFCHGDTSSCQAIVDTGTSLIAGPTMDILILQQLIGATPTPAGEFLVDCSRIKSLPVISFVLNGVEYSLPGESYIRKDVVGDRVLCLTGFVAVDIVSPKGPLWILGDVFLSQVYSIYDRGQDRVGFAQLKVPS 405
BLAST of Lysosomal aspartic protease vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007388.1 (pep scaffold:Pmarinus_7.0:GL480547:23667:25044:-1 gene:ENSPMAG00000006669.1 transcript:ENSPMAT00000007388.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 78.5666 bits (192), Expect = 6.399e-18 Identity = 50/133 (37.59%), Postives = 75/133 (56.39%), Query Frame = 2
Query: 860 SISMLG-KTYC-TNCTAIADTGTSLIVGPQSIIDDINSKFNGTKIN-GMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLSGIP-----FFILGDVFIGRYYTEFDLGNSRIGFA 1225
SIS++G + C + C AI DTGTS+I GP + + T + G I C ++ LP I + + G+ F L Y++ +Q T+C+ GF + +P +ILGDVFIG++YTEFD + R+GFA
Sbjct: 1 SISVVGGPSLCVSGCPAIVDTGTSVIAGPSGPLRILQVALGATALPWGQYTIDCRAIPTLPDIVLTLGGKPFHLSGKQYVIVDETVQGTLCLLGFMVMD-VPSSEGLLWILGDVFIGKFYTEFDRDHDRVGFA 132
BLAST of Lysosomal aspartic protease vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006274.1 (pep scaffold:Pmarinus_7.0:GL484715:1746:11685:1 gene:ENSPMAG00000005661.1 transcript:ENSPMAT00000006274.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 63.1586 bits (152), Expect = 1.021e-11 Identity = 48/151 (31.79%), Postives = 72/151 (47.68%), Query Frame = 2
Query: 266 IPEPLDNYMDAQ---YFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVV--AKFDGILGMAYPSISV--DHVTPVFQNMVAQ 697
+P LDN Y+ E+ +GTPPQ +L DTGSSN V + R Y +SSTY + ++ + Y G+ G L TD V V S A + +F + + ++GILG+AY +I+ D + P F ++V Q
Sbjct: 65 VPGMLDNLRGKPGQGYYIEMALGTPPQMLNILVDTGSSNFAVGAAPH------LFLKRYYRPERSSTYRPSHSSVYVPYTQGNWQGDLGTDLVSVPHGGNSSVRANVAAITYSNSFFINGSNWEGILGLAYANIARPDDSLEPFFDSLVKQ 209
BLAST of Lysosomal aspartic protease vs. Ensembl Yeast
Match: PEP4 (Vacuolar aspartyl protease (proteinase A); required for posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates [Source:SGD;Acc:S000006075]) HSP 1 Score: 264.233 bits (674), Expect = 7.301e-85 Identity = 142/325 (43.69%), Postives = 194/325 (59.69%), Query Frame = 2
Query: 275 PLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYL-NRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYC--TNCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGI----PFFILGDVFIGRYYTEFDLGNSRIGFA 1225
PL NY++AQY+ +IT+GTPPQ F V+ DTGSSNLWVPS +C +LAC H KYD SS+Y NGT F+I+YGTGSL G++S DT+ + + Q F EA EPG TF KFDGILG+ Y +ISVD V P F N + Q L+D F+FYL + + + GGE FGG D + ++G T+ PV K YW+ + I LG Y + A DTGTSLI P + + IN++ K G + C++ LP + G NF + P DY L++ + C+S T + P I+GD F+ +YY+ +DLGN+ +G A
Sbjct: 82 PLTNYLNAQYYTDITLGTPPQNFKVILDTGSSNLWVPSNECG--SLACFLHSKYDHEASSSYKANGTEFAIQYGTGSLEGYISQDTLSIGDLTIPKQDFAEATSEPGLTFAFGKFDGILGLGYDTISVDKVVPPFYNAIQQDLLDEKRFAFYLGDTSKDTENGGEATFGGIDESKFKGDITWLPVRRKAYWEVKFEGIG-LGDEYAELESHGAAIDTGTSLITLPSGLAEMINAEIGAKKGWTGQYTLDCNTRDNLPDLIFNFNGYNFTIGPYDYTLEVSGS-CISAITPMDFPEPVGPLAIVGDAFLRKYYSIYDLGNNAVGLA 402
BLAST of Lysosomal aspartic protease vs. Ensembl Yeast
Match: BAR1 (Aspartyl protease; secreted into the periplasmic space of mating type a cell; helps cells find mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest [Source:SGD;Acc:S000001277]) HSP 1 Score: 86.2705 bits (212), Expect = 1.296e-18 Identity = 96/366 (26.23%), Postives = 151/366 (41.26%), Query Frame = 2
Query: 278 LDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWV----------PSKKCKLTN-----------LACLTHRKYDSSKSST--YVKNGTAFSIRYGTGSLS-GFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISV--------DHVTPVF-QNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGP-FTY-------TPVTSKTYWQFSMQSISMLGKTYCTNCT-------AIADTGTSLIVGPQSIIDDINSKFNG--TKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
L + + Y + IGTP Q TVLFDTGS++ WV S +N + C + Y+ +SST Y++NG F I Y G+ + G T+TV + + + FG A + G+LG+ +P + P F Q + ++K++D +S +LN +G G I+FG D + + G FT+ T V + ++Q + K+ C + T + D+GTSL+ P+ I D + S N ++ G+ I+ C N + S IL + GF +LGDVF+ Y FDL N +I A
Sbjct: 37 LQHEEEMYYATTLDIGTPSQSLTVLFDTGSADFWVMDSSNPFCLPNSNTSSYSNATYNGEEVKPSIDCRSMSTYNEHRSSTYQYLENG-RFYITYADGTFADGSWGTETVSINGIDIPNIQFGVAK------YATTPVSGVLGIGFPRRESVKGYEGAPNEYYPNFPQILKSEKIIDVVAYSLFLNSPDSGT--GSIVFGAIDESKFSGDLFTFPMVNEYPTIVDAPATLAMTIQGLGAQNKSSCEHETFTTTKYPVLLDSGTSLLNAPKVIADKMASFVNASYSEEEGIYILDCPVSVGDVEYNFDFGDLQISVPLSSLILSPETEGSYCGFAVQPTNDSMVLGDVFLSSAYVVFDLDNYKISLA 393
BLAST of Lysosomal aspartic protease vs. Ensembl Yeast
Match: YPS3 (Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor [Source:SGD;Acc:S000004111]) HSP 1 Score: 85.5001 bits (210), Expect = 2.064e-18 Identity = 93/353 (26.35%), Postives = 146/353 (41.36%), Query Frame = 2
Query: 302 YFGEITIGTPPQPFTVLFDTGSSNLWVPSKK---CKLTNLACLTHRKYDSSKSSTYVKNGTA-FSIRYGTGSLS-GFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISV---------DHVTPVFQN----MVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVT----SKTYWQFSMQSISMLG---KTYCTNCT-------AIADTGTSLIVGPQSIIDDINSKFNG--TKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETL 1258
Y E+ IGTP Q TVL DTGS++LWVP K C + + C + +D +KSST+ N ++ F YG G+ + G D + + +F A E +TF G+LG+ ++ V D + + N + +DA +S +LN + + G I+FG D + Y+G P+ S+ Y +++ G +T N T A+ D+GT+L P + + N +K G C S + G SD+ ++ CV +G ILGD F+ Y +DL N I A K + E +
Sbjct: 63 YSVELAIGTPSQNLTVLLDTGSADLWVPGKGNPYCG-SVMDCDQYGVFDKTKSSTFKANKSSPFYAAYGDGTYAEGAFGQDKLKYNELDLSGLSFAVAN-ESNSTF------GVLGIGLSTLEVTYSGKVAIMDKRSYEYDNFPLFLKHSGAIDATAYSLFLNDE--SQSSGSILFGAVDHSKYEGQLYTIPLVNLYKSQGYQHPVAFDVTLQGLGLQTDKRNITLTTTKLPALLDSGTTLTYLPSQAVALLAKSLNASYSKTLGYYEYTCPSSDNKTSVAFDFGGFRINAPLSDFTMQTSVGTCVLAIIPQAGNATAILGDSFLRNAYVVYDLDNYEISLAQAKYGTGKENV 405
BLAST of Lysosomal aspartic protease vs. Ensembl Yeast
Match: YPS1 (Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor [Source:SGD;Acc:S000004110]) HSP 1 Score: 62.3882 bits (150), Expect = 5.374e-11 Identity = 86/335 (25.67%), Postives = 140/335 (41.79%), Query Frame = 2
Query: 359 TGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSL-SGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISV----------------DHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTS-------KTYWQFSMQ----SISMLGKTYCTNCT----AIADTGTSLIVGPQSIIDDINSKFNG---TKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETL 1258
+G++ VP+ + + C + + +S SST+ N T FSI YG G+ SG TD + ++ V +F A E +T G+LG+ P + V D+ V +N A K + +S YLN + G I+FG D + Y G P+ + + QF + IS G + T T A+ D+GT+L PQ+++ I ++ ++I G ++ C S + ++ G + S +IL T C+ G S ILGD F+ Y +DL N I A + + E +
Sbjct: 170 SGTATQSVPASEATMD---CQQYGTFSTSGSSTFRSNNTYFSISYGDGTFASGTFGTDVLDLSDLNVTGLSFAVAN-ETNSTM------GVLGIGLPELEVTYSGSTASHSGKAYKYDNFPIVLKNSGAIK---SNTYSLYLND--SDAMHGTILFGAVDHSKYTGTLYTIPIVNTLSASGFSSPIQFDVTINGIGISDSGSSNKTLTTTKIPALLDSGTTLTYLPQTVVSMIATELGAQYSSRI-GYYVLDCPSDDSMEIV-FDFGGFHINAPLSSFILS-TGTTCLLGIIPTSDDTGTILGDSFLTNAYVVYDLENLEISMAQARYNTTSENI 486
BLAST of Lysosomal aspartic protease vs. Ensembl Yeast
Match: YPS6 (Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance [Source:SGD;Acc:S000001478]) HSP 1 Score: 58.9214 bits (141), Expect = 7.860e-10 Identity = 89/383 (23.24%), Postives = 151/383 (39.43%), Query Frame = 2
Query: 302 YFGEITIGTPPQPFTVLFDTGSSNLWVPSKK---CK---------------LT----------------NLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGS-LSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYP----------SISVDHVTPVFQN----MVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVT-------SKTYWQFSMQSISMLGKTYCTNCTA------IADTGTSLIVGPQSIIDDINSKFNG--TKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETL 1258
Y ++ IGTPPQ + DTGSS++ V + CK LT N C +D+S SST+ N T F+ YG G+ +G TD V + TFG + G GILG++ P +++++ ++ N + Q ++ +S +LN G I+FG D + Y G P+ S + QS+++L + + D+GT+ P I + I F+G + + I CS V+ ++++ G N S+++ +D ++ S S ++LGD F+ Y +DL N I A +++E +
Sbjct: 67 YVVKMEIGTPPQTLYLQLDTGSSDMIVNNADIAYCKSMSDGSDYASTDNYELTATFNGLPSTTISSEAYNTLCSYWGTFDASNSSTFENNATFFNNTYGDGTYYAGTYGTDVVSFENITLNDFTFGVSNDTIGNP------SGILGISLPIAEFTDGIEYALALNRTPFIYDNFPMELKNQGKINKIAYSLFLNGP--DAHFGSILFGAVDKSKYTGQLYTLPMLQAFNTLGSNPGMIITAQSVAILDSESGNKTVSDIQFPVMLDSGTTFSYLPTEIAEAIGKSFDGEYSSDDQGYIFDCSKVND-TLLSVDFGGFNISANISNFVTSAKDRCVLNVKQSEST---YMLGDAFLVDAYVVYDLENYEISIAQASFNNQEEDI 437
BLAST of Lysosomal aspartic protease vs. Ensembl Nematostella
Match: EDO49162 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH56]) HSP 1 Score: 406.757 bits (1044), Expect = 1.181e-140 Identity = 211/366 (57.65%), Postives = 252/366 (68.85%), Query Frame = 2
Query: 170 RVPLKKMESARSNI----VKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTK--INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK---LQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
R+PL KM + R ++ + +L + +PEPL NYMDAQY+GEITIGTPPQ FTV+FDTGSSNLWVPSKKC TN+ACL H KYDS+KSSTY KNGT F+IRYG+GSLSGFLS DTV V V QTF EA+ EPG TFV AKFDGILGM + SISVD V PVF +MV QKLV AP+FSFYLNR+ PGGE++ GGSDP YY+G F+Y PVT + YWQF M IS+ ++C++ C AIADTGTSLI GP I+ +N+ G K I G + CS++ LP I I G+ + L YILK L +VC+SGF L P +ILGDVFIG YYTEFD GN R+GFA
Sbjct: 3 RIPLHKMPTPRQSLKEVGISVEQLLGKYGGKYEGGDVPEPLINYMDAQYYGEITIGTPPQKFTVVFDTGSSNLWVPSKKCSWTNIACLLHDKYDSTKSSTYKKNGTEFAIRYGSGSLSGFLSIDTVSVGGIDVKGQTFAEALKEPGLTFVAAKFDGILGMGFSSISVDQVVPVFYDMVLQKLVPAPVFSFYLNREPGASPGGELLLGGSDPKYYKGNFSYVPVTQEGYWQFKMDGISVKEGSFCSDGCQAIADTGTSLIAGPTDEIEKLNN-LIGAKIIIGGEYTVNCSAIDSLPDITFTIGGKKYVLTGKQYILKVTTLGQSVCISGFLGLDVPPPRGPLWILGDVFIGPYYTEFDFGNKRVGFA 367
BLAST of Lysosomal aspartic protease vs. Ensembl Nematostella
Match: EDO37418 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFW0]) HSP 1 Score: 80.8777 bits (198), Expect = 2.211e-16 Identity = 68/219 (31.05%), Postives = 105/219 (47.95%), Query Frame = 2
Query: 302 YFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCK-LTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGE-AMVEPGTTFVVAKF--DGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTN----CTAIADTGTSLIV 934
Y E+ IGTPPQ L DTGSSN+ + KC+ C Y KS++ +YG G+ SG + D +VA + T E A++ F + +GILG+AY S++ D VTP++ +V +D + + + NG G+I +P Y GP YT + +T++ SM I + G + + TAI D+GT+ I+
Sbjct: 181 YVIELDIGTPPQKLEFLIDTGSSNMAIAGPKCQDQWGSKCKIDNFYFPDKSTSARDIKIPIETQYGKGAWSGDMYQD--VVAFPENGPNTLVEFAVIRNEKDFFLRNSINEGILGLAYRSLATDGVTPLYDQLV----LDGKVPNVFTLGLCNGM--GQIWLDHPEPGTYVGPIHYTEIEQETWYTVSMTGIDVAGNSLDLSPQVFPTAIVDSGTTDIL 391
BLAST of Lysosomal aspartic protease vs. Ensembl Nematostella
Match: EDO39359 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SA91]) HSP 1 Score: 77.7962 bits (190), Expect = 1.540e-15 Identity = 84/363 (23.14%), Postives = 147/363 (40.50%), Query Frame = 2
Query: 302 YFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVV--AKFDGILGMAYPSISV--DHVTPVFQNMVAQKLVDAPIFSFYL----NRDVNGKP--GGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNC------TAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTII----------LCSSVSKLP----------VINIQIAGRNFPLK--PSDYILKLQDTVCVSG-----FTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETLY 1261
Y+ +G+PPQ VL DTGSSN V + + KS++Y S+ Y GS G L +D + V+ A + F + + ++GILG+ Y ++ VTPVF V V+ FS L D + +P G ++FG D Y+G YTP+ K Y++ + +++ G + +C I D+GT+ + + + + I + K + + +I +C + + P +++ + + F LK P Y+ + + + G F ++G V + +Y FD N ++GFA K+ Y
Sbjct: 12 YYIASNLGSPPQRINVLVDTGSSNFAVAASAHPYIPYY------FHIDKSTSYKDLNKPVSVPYTQGSWEGELGSDLIKFVDGPNVTLRVDVASILQSENFFINGSMWEGILGLGYARLAKPDSSVTPVFDQFVLDHGVEKDSFSMQLCGSSEIDPSAEPTVAGTMVFGEIDEELYKGDLLYTPIVKKWYYEVVITDVAVDGDSLALDCKKYNYDKTIVDSGTTNLRVSEEVFNAILDRLR--KFDKVNLIPDDFWYGRQMMCWNYDRTPWEEFPDMSISLLSSLSSSKEFRLKIPPQLYLRETIEHGPIMGQHCYKFAITRAERGTVIGAVIMEGFYVVFDRENVQVGFAATTCGGKEPMRY 366
BLAST of Lysosomal aspartic protease vs. Ensembl Medaka
Match: ctsd (cathepsin D [Source:NCBI gene;Acc:111946846]) HSP 1 Score: 369.007 bits (946), Expect = 5.248e-125 Identity = 189/391 (48.34%), Postives = 250/391 (63.94%), Query Frame = 2
Query: 125 LIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFM--KSEIK---------NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
+I AL ++G L+R+PLKK S R + + + K +K P PE L NY+DAQY+GEI +GTPPQPFTV+FDTGSSNLWVPS C L ++ACL H KY+S+KSSTYVKNGT+FSI+YG+GSLSG+LS DT + V +Q FGEA+ +PG F+ AKFDGILGMAYP ISVD V PVF N++ QK VD+ +FSFYLNR+ + +PGGE++ GG+DP YY G F Y ++ + YWQ M +++ + + C C AI DTGTSL+ GP + + + I G +I C + LP I I G+++ L Y+LK T+C+SGF L P +ILGDVFIG+YYT FD ++R+GFA K
Sbjct: 8 VIAALALSG--EALIRIPLKKFRSIRRELTDSGREAHELLADKHSLKYNLGFPSSNGPTPETLKNYLDAQYYGEIALGTPPQPFTVVFDTGSSNLWVPSVHCSLLDIACLLHHKYNSAKSSTYVKNGTSFSIQYGSGSLSGYLSQDTCTIGDISVENQVFGEAIKQPGVAFIAAKFDGILGMAYPRISVDGVVPVFDNIMQQKKVDSNVFSFYLNRNPDTEPGGELLLGGTDPKYYSGDFHYVNISRQAYWQIHMDGMAVGSQLSLCKGGCEAIVDTGTSLLTGPSAEVKALQKAIGAIPLIQGEYMINCDKIPSLPAITFNIGGQSYTLTGDQYVLKESQAGKTICLSGFMGLDIPAPAGPLWILGDVFIGQYYTVFDRDSNRVGFAKSK 396
BLAST of Lysosomal aspartic protease vs. Ensembl Medaka
Match: ENSORLT00000000117.2 (cathepsin D [Source:NCBI gene;Acc:101161712]) HSP 1 Score: 369.007 bits (946), Expect = 5.248e-125 Identity = 189/391 (48.34%), Postives = 250/391 (63.94%), Query Frame = 2
Query: 125 LIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFM--KSEIK---------NPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCT-NCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQD---TVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
+I AL ++G L+R+PLKK S R + + + K +K P PE L NY+DAQY+GEI +GTPPQPFTV+FDTGSSNLWVPS C L ++ACL H KY+S+KSSTYVKNGT+FSI+YG+GSLSG+LS DT + V +Q FGEA+ +PG F+ AKFDGILGMAYP ISVD V PVF N++ QK VD+ +FSFYLNR+ + +PGGE++ GG+DP YY G F Y ++ + YWQ M +++ + + C C AI DTGTSL+ GP + + + I G +I C + LP I I G+++ L Y+LK T+C+SGF L P +ILGDVFIG+YYT FD ++R+GFA K
Sbjct: 8 VIAALALSG--EALIRIPLKKFRSIRRELTDSGREAHELLADKHSLKYNLGFPSSNGPTPETLKNYLDAQYYGEIALGTPPQPFTVVFDTGSSNLWVPSVHCSLLDIACLLHHKYNSAKSSTYVKNGTSFSIQYGSGSLSGYLSQDTCTIGDISVENQVFGEAIKQPGVAFIAAKFDGILGMAYPRISVDGVVPVFDNIMQQKKVDSNVFSFYLNRNPDTEPGGELLLGGTDPKYYSGDFHYVNISRQAYWQIHMDGMAVGSQLSLCKGGCEAIVDTGTSLLTGPSAEVKALQKAIGAIPLIQGEYMINCDKIPSLPAITFNIGGQSYTLTGDQYVLKESQAGKTICLSGFMGLDIPAPAGPLWILGDVFIGQYYTVFDRDSNRVGFAKSK 396
BLAST of Lysosomal aspartic protease vs. Ensembl Medaka
Match: ENSORLT00000016892.2 (cathepsin D [Source:NCBI gene;Acc:101168958]) HSP 1 Score: 350.903 bits (899), Expect = 6.457e-118 Identity = 191/391 (48.85%), Postives = 240/391 (61.38%), Query Frame = 2
Query: 122 LLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHF---------MKSEIKNPIP-EPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYC-TNCTAIADTGTSLIVGPQSIIDDINSKFNGTK-INGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILK---LQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
L II ++ G L+RVPL K S R + M L+ + S +P E L N+MDAQY+G I+IGTPPQ F+VLFDTGSSNLWVPS C ++AC HR+Y+S KSS+YVKNGT FSIRYG GSLSGF+S DTV VA V Q FGEA+ +PG TF VA+FDG+LGMAYPSISV +VTPVF +A KL+ IFS Y++RD + GGE+I GG DP Y+ G Y VT K YWQ M + + + T C C +I DTGTSL+VGP I ++ + G I C + LPVI+ I G+ F L DYILK + ++C+SGF ++ P +ILGDVFIG+YYT FD R+GFA K
Sbjct: 7 LCIIGALMMGQSTALIRVPLHKTRSLRRLMSDNGMSLDDLRALGMRVGSLDSSASPELPVERLTNFMDAQYYGLISIGTPPQNFSVLFDTGSSNLWVPSIHCSFLDVACWVHRRYNSKKSSSYVKNGTEFSIRYGRGSLSGFISQDTVSVAGLSVPGQQFGEAVKQPGITFAVARFDGVLGMAYPSISVANVTPVFDTAMAAKLLPQNIFSVYISRDTAAEVGGELILGGIDPQYFSGDLHYVNVTRKAYWQIQMDRVDVGNQLTLCKAGCQSIVDTGTSLMVGPAEEIRALHKAIGALPLLMGEYFIDCKKIPSLPVISFNIGGKTFNLTGEDYILKESQMGASICLSGFMAMDIPPPAGPLWILGDVFIGKYYTVFDRNADRVGFAAAK 397
BLAST of Lysosomal aspartic protease vs. Ensembl Medaka
Match: ren (renin [Source:NCBI gene;Acc:101168210]) HSP 1 Score: 292.738 bits (748), Expect = 3.479e-95 Identity = 158/377 (41.91%), Postives = 221/377 (58.62%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNHFMK-------SEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKT-YCTN-CTAIADTGTSLIVGPQS----IIDDINSKFN--GTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYIL---KLQDTVCVSGFTSLS----GIPFFILGDVFIGRYYTEFDLGNSRIGFA 1225
L R+ LKKM S R + + + + H + ++ P PL NY+D QYFGEI+IG+P Q F V+FDTGS+NLWVPS+ C + AC TH +YD+S S TYV+NGT FSI+Y +G++ GFLS D V V V Q F EA F+ AKFDG+LGM YP +++D +TPVF +++Q ++ +FS Y +RD PGGE++ GG+DPNYY G F Y W+ M+ +S+ +T +C CTA+ DTG+S I GP S +++ I + + G K+N C +V LP + + G+ + L DYIL +++ VC F L P +ILG FI RYYTEFD ++RIGFA
Sbjct: 23 ALRRIALKKMPSIRETLQEMGVSVEHVLTELAQMKPADNNGTAPTPLTNYLDTQYFGEISIGSPAQMFNVVFDTGSANLWVPSQSCSPFSTACFTHNRYDASHSRTYVENGTGFSIQYASGNVRGFLSEDVV-VVGGIPVVQVFAEATSLSAMPFIFAKFDGVLGMGYPDVAIDGITPVFDRIMSQHVLKEEVFSVYYSRDPKHSPGGELVLGGTDPNYYTGSFNYVKTKDTGKWEVLMKGVSVGTRTMFCAEGCTAVIDTGSSYITGPASSVSVLMETIGADLDESGYKVN------CDTVKLLPSVTFHLGGQQYSLTHEDYILWQSQIEGEVCTVTFRGLDVPPPTGPLWILGANFIARYYTEFDRRHNRIGFA 392
BLAST of Lysosomal aspartic protease vs. Ensembl Medaka
Match: nots (cathepsin D-like [Source:NCBI gene;Acc:101159061]) HSP 1 Score: 286.96 bits (733), Expect = 9.585e-93 Identity = 158/389 (40.62%), Postives = 225/389 (57.84%), Query Frame = 2
Query: 161 GLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPI--------------------PEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGK-PGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLG-KTYCTN-CTAIADTGTSLIVGPQSIIDDINSKFNGTKIN-GMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKL---QDTVCVSGFTSLSGI----PFFILGDVFIGRYYTEFDLGNSRIGFAPVK 1234
LLRVPL++ + R+ + + +L+ F+K + E + N+MDAQY+GEI +GTP Q F+V+FDTGSS+LWVPS C + AC HR + + KSS++ +G F I YG+G L G + DT+ + V++Q FGE++ EPG+TFV AKFDG+LG+AYPS++ PVF NM+AQK++D PIFSFYL+R + P G+++ GG+D + Y GP + PVT K YWQ M S+S+ G + C C AI DTGTSLI GP I ++ T + G ++ C+ +S LP + + + L YI K +C +GF + P +ILGDVF+ +YYT FD G R+GFA K
Sbjct: 17 ALLRVPLRRNPTIRTQM-RAEGLLDQFLKDNQPDTFNRRYAQCFPPGTQSLRLGRSSEKIYNFMDAQYYGEIRLGTPEQNFSVIFDTGSSDLWVPSSYC--VSQACAFHRHFKAFKSSSFHHDGRTFGIHYGSGHLLGVMGKDTLRIGNLTVLNQEFGESVYEPGSTFVTAKFDGVLGLAYPSLAEIIGKPVFDNMLAQKILDEPIFSFYLSRSKSKSVPEGQLLLGGTDESLYSGPINWVPVTIKGYWQIRMDSVSVQGVSSLCRRGCEAIVDTGTSLIAGPPREILRLHQLIGATPTHFGDFVVDCARLSSLPHVTFVLGEVEYTLTSEHYIRKETFSSRELCFTGFMAAEMFSADGPLWILGDVFLTQYYTIFDKGQDRVGFARAK 402
BLAST of Lysosomal aspartic protease vs. Planmine SMEST
Match: SMESG000035344.1 (SMESG000035344.1) HSP 1 Score: 793.112 bits (2047), Expect = 0.000e+0 Identity = 388/389 (99.74%), Postives = 389/389 (100.00%), Query Frame = 2
Query: 110 MNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETLYYKPLF 1276
MN+KLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETLYYKPLF
Sbjct: 1 MNVKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIKNPIPEPLDNYMDAQYFGEITIGTPPQPFTVLFDTGSSNLWVPSKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVVSQTFGEAMVEPGTTFVVAKFDGILGMAYPSISVDHVTPVFQNMVAQKLVDAPIFSFYLNRDVNGKPGGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGKTYCTNCTAIADTGTSLIVGPQSIIDDINSKFNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLSGIPFFILGDVFIGRYYTEFDLGNSRIGFAPVKTPSKDETLYYKPLF 389
BLAST of Lysosomal aspartic protease vs. Planmine SMEST
Match: SMESG000037819.1 (SMESG000037819.1) HSP 1 Score: 57.3806 bits (137), Expect = 1.275e-8 Identity = 83/312 (26.60%), Postives = 134/312 (42.95%), Query Frame = 2
Query: 302 YFGEITIGTPPQPFTVLFDTGSSNLWVP-SKKCKLTNLACLTHRKYDSSKSSTYVKNGTAFSIRYGTGSLSGFLSTDTVIVATAKVV----SQTFGE--------AMVEPGTTFVV--AKFDGILGMAYPSIS----VDHVTPVFQNMVAQKLVDAPIFSFYL---NRDVNGKP--GGEIIFGGSDPNYYQGPFTYTPVTSKTYWQFSMQSISMLGK-TYCTNC------TAIADTGTSLIVGPQSIIDDINSK--FNGTKINGMTIILCSSVSKLPVINIQIAGRNFPLKPSDYILKLQDTVCVSGFTSLS 1138
Y+ + IG+PPQ F ++ DTGSSN V S + L+N + S SSTYV + + + Y +G +G S+D + +Q + + +++ TTF F GILG+AYP ++ + H + +F ++V K +FS L NR+ +P G I GG + + Y P + K +++ + IS+ K ++C +I DTGT+ P I + S FN I + VI I N P PS Y ++Q ++ +SL
Sbjct: 46 YYMTVGIGSPPQKFNLVIDTGSSNTAVAGSPQQFLSNY-------FKSQLSSTYVDHKISVGVPYDSGKWNGLYSSDIFTFEGVAYIDANKTQRYSKEINVRSNFGLIKESTTFFPKNGNFQGILGLAYPVLAQPANLIHKSTLFDDVV-MKYGLPNMFSIVLCGHNRN-ESQPTTNGFFIAGGKNDSLYNPPLVDILIRRKWFYEIIISRISISNKPIILSSCKEYNRDKSILDTGTADTYFPNVIFHSLVSSIIFNMKNAPFKVTIHSPFWDRRAVICIL---SNSPNHPSTYFPEIQIEFVINDTSSLE 345 The following BLAST results are available for this feature:
BLAST of Lysosomal aspartic protease vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Lysosomal aspartic protease vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Lysosomal aspartic protease vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Lysosomal aspartic protease vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Lysosomal aspartic protease vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014243 ID=SMED30014243|Name=Lysosomal aspartic protease|organism=Schmidtea mediterranea sexual|type=transcript|length=1340bpback to top protein sequence of SMED30014243-orf-1 >SMED30014243-orf-1 ID=SMED30014243-orf-1|Name=SMED30014243-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=391bp MMNIKLLIIALVINGIFGGLLRVPLKKMESARSNIVKFNMMLNHFMKSEIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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