Myeloid differentiation primary response protein MyD88

Overview
NameMyeloid differentiation primary response protein MyD88
Smed IDSMED30014235
Length (bp)1401
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Myeloid differentiation primary response protein MyD88 (SMED30014235) t-SNE clustered cells

Violin plots show distribution of expression levels for Myeloid differentiation primary response protein MyD88 (SMED30014235) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Myeloid differentiation primary response protein MyD88 (SMED30014235) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Myeloid differentiation primary response protein MyD88 (SMED30014235) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30014235

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30014235h1SMcG0009662 Contig6231newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30014235h1SMcG0009662 Contig6231uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
reproductive organSMED30014235h1SMcG0009662 Contig31270newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30014235h1SMcG0009662 Contig31270uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30014235h1SMcG0009662 Contig40753uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30014235h1SMcG0009662 Contig40753newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Zebrafish
Match: myd88 (MYD88 innate immune signal transduction adaptor [Source:NCBI gene;Acc:403145])

HSP 1 Score: 55.8398 bits (133), Expect = 2.970e-8
Identity = 57/262 (21.76%), Postives = 116/262 (44.27%), Query Frame = 3
Query:  297 SVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESR--GLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKP 1076
            ++P+ AL+   ++KL      F+ P   V   D+R +A L++++ LE+     +KR      ++ D       T  +L   L+K +R D+I   +E L  D  +++++  +           K +  P       +T  +   T   +      + +D FI +      DI+ +  +  +LE     L +       + G C+    +  I  R K+++++IS  +++S++C  Q  FA      +R  ++IPV  + +K+P
Sbjct:   11 AIPVTALNCSFRKKLGL----FLNPTNTVAA-DWRTVAELMDFTYLEIKNF--EKRECPFEKVLTDWETRPDATVANLLSILEKAERKDVISELKEILDDDCRKYMERQQR-----------KPLQVPVVDSCGPRTQEREGITLYDDPQGLTPETFDAFICY---CQSDIQFVHEMIKQLEHTEYNLKLCVFDRDVLPGTCVWTIASELIEKRCKRMVVVISDDYLDSDACDFQTKFALSLCPGARTKRLIPVVYKSMKRP 251          
BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Xenopus
Match: myd88 (myeloid differentiation primary response 88 [Source:NCBI gene;Acc:549591])

HSP 1 Score: 51.6026 bits (122), Expect = 1.264e-6
Identity = 67/298 (22.48%), Postives = 129/298 (43.29%), Query Frame = 3
Query:  291 FSSVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRM--KMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRG--LTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGF----NYYNNSIREMFFDSFINSF 1160
             +S+P+ AL+   + +L      ++ P+  V    +  LA  + Y  LE+      +R+   T  +L+    K  + T   L   LKK++R+DI+ +    +  D M+ L+K    L    ++    +    T+ D+   ++ + F               D FI + ++ I  ++ M  IS RLE     L +       + G C+    +  I +R +K++++IS  +++S  C  Q  FA      +R  ++IPV  + +K+P    SI  F    +Y N   +  F++    + 
Sbjct:   12 MNSIPLVALNYNVRHRLSL----YLNPNAVVA-AGWTQLAEEMGYDYLEIKNF---ERFPDCTMKLLEDWEKKCFRATVGGLLEMLKKMERNDILTDLGPLIEADCMKHLEKKNAPLPLQDDKVDSSEQYRITKSDDPSGSLPETF---------------DAFICYCAQDISFVQEM--IS-RLEQTDYKLKLCVFDRDVLPGTCLWSITSELIEHRCRKMVVIISDDYLDSSECDFQTKFALSLGPGAREKRLIPVKYKPMKRP--FPSILRFITLCDYTNPCTKGWFWERLAKAL 281          
BLAST of Myeloid differentiation primary response protein MyD88 vs. UniProt/SwissProt
Match: sp|Q9DF60|MY88A_XENLA (Myeloid differentiation primary response protein MyD88-A OS=Xenopus laevis OX=8355 GN=myd88-a PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 5.516e-11
Identity = 69/296 (23.31%), Postives = 129/296 (43.58%), Query Frame = 3
Query:  291 FSSVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRM--KMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRG--LTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKK--PECIASINGFNYYNNSIREMFFDSFINSF 1160
             +S+P+ AL+   + +L    C ++ PD  V   D+  LA  + Y  LE+      +RY   T  +L+    K  + T   L   LKK++R+DI+ +    +  D M++L+K              K +  P + D+   +  +   T   +      + +D FI + ++ I  ++ M     RLE     L +       + G C+    +  I NR +K++++IS  +++S  C  Q  FA      +R  ++IPV  + +K+  P  +  I   +Y N   +  F+D    + 
Sbjct:   12 MNSIPLVALNYTVRHRL----CLYLNPDAVVA-ADWTRLAEEMGYDYLEIRNF---QRYPDSTMKLLEDWEKKCFRATVGGLLEMLKKMERNDILTDLGPLIEADCMKYLEK--------------KHVPLPIQDDKVDSS-EQYRITKSDDPYGSMPETFDAFICYCAQDISFVQEM---ISRLEQTDYKLKLCVFDRDVLPGTCLWSITSELIENRCRKMVVIISDDYLDSSDCDFQTKFALSLGPGAREKRLIPVKYKPMKRPFPSILRFITVCDYTNPYTKAWFWDKLAKAL 281          
BLAST of Myeloid differentiation primary response protein MyD88 vs. UniProt/SwissProt
Match: sp|Q5FWM2|MY88B_XENLA (Myeloid differentiation primary response protein MyD88-B OS=Xenopus laevis OX=8355 GN=myd88-b PE=2 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 3.935e-8
Identity = 67/273 (24.54%), Postives = 122/273 (44.69%), Query Frame = 3
Query:  273 MNISDYFSSVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILD--RMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFL-KKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRG--LTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKP 1076
            MN  D  +S+P+ AL+   + +L    C ++ P+  V   D+  LA  + Y  LE+       RY   T  +L+  + K  + T   L   LKK++R+DI+ +    +  D  ++L KK G    +  N ++ +Q      + ++    G +  T            +D FI   ++   DI  +  +  RLE     L +       + G C+    +  I NR +K++++IS  +++S  C  Q  FA      +R  ++IPV  + +K+P
Sbjct:    7 MNSFD-MNSIPLVALNYTVRHRL----CLYLNPNAVVA-ADWTRLAEEMGYDYLEIRNF---DRYPDSTMKLLEDWQKKCFRATVGGLLEMLKKMERNDILTDLAPLIEADCKKYLEKKHGPLPLQDDNVDSSEQY----RITKSDDPYGSMPET------------FDAFICCCAQ---DILFVQEMISRLEQTDYKLKLCVFDRDVLPGTCLWSITSELIENRCRKMVVIISDDYLDSSECDFQTKFALSLGPGAREKRLIPVKYKPMKRP 251          
BLAST of Myeloid differentiation primary response protein MyD88 vs. UniProt/SwissProt
Match: sp|Q5XJ85|MYD88_DANRE (Myeloid differentiation primary response protein MyD88 OS=Danio rerio OX=7955 GN=myd88 PE=2 SV=2)

HSP 1 Score: 55.8398 bits (133), Expect = 2.764e-7
Identity = 57/262 (21.76%), Postives = 116/262 (44.27%), Query Frame = 3
Query:  297 SVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESR--GLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKP 1076
            ++P+ AL+   ++KL      F+ P   V   D+R +A L++++ LE+     +KR      ++ D       T  +L   L+K +R D+I   +E L  D  +++++  +           K +  P       +T  +   T   +      + +D FI +      DI+ +  +  +LE     L +       + G C+    +  I  R K+++++IS  +++S++C  Q  FA      +R  ++IPV  + +K+P
Sbjct:   11 AIPVTALNCSFRKKLGL----FLNPTNTVAA-DWRTVAELMDFTYLEIKNF--EKRDCPFEKVLTDWETRPDATVANLLSILEKAERKDVISELKEILDDDCRKYMERQQR-----------KPLQVPVVDSCGPRTQEREGITLYDDPQGLTPETFDAFICY---CQSDIQFVHEMIKQLEHTEYNLKLCVFDRDVLPGTCVWTIASELIEKRCKRMVVVISDDYLDSDACDFQTKFALSLCPGARTKRLIPVVYKSMKRP 251          
BLAST of Myeloid differentiation primary response protein MyD88 vs. UniProt/SwissProt
Match: sp|Q28DJ2|MYD88_XENTR (Myeloid differentiation primary response protein MyD88 OS=Xenopus tropicalis OX=8364 GN=myd88 PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 5.688e-6
Identity = 67/298 (22.48%), Postives = 129/298 (43.29%), Query Frame = 3
Query:  291 FSSVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRM--KMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRG--LTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGF----NYYNNSIREMFFDSFINSF 1160
             +S+P+ AL+   + +L      ++ P+  V    +  LA  + Y  LE+      +R+   T  +L+    K  + T   L   LKK++R+DI+ +    +  D M+ L+K    L    ++    +    T+ D+   ++ + F               D FI + ++ I  ++ M  IS RLE     L +       + G C+    +  I +R +K++++IS  +++S  C  Q  FA      +R  ++IPV  + +K+P    SI  F    +Y N   +  F++    + 
Sbjct:   12 MNSIPLVALNYNVRHRLSL----YLNPNAVVA-AGWTQLAEEMGYDYLEIKNF---ERFPDCTMKLLEDWEKKCFRATVGGLLEMLKKMERNDILTDLGPLIEADCMKHLEKKNAPLPLQDDKVDSSEQYRITKSDDPSGSLPETF---------------DAFICYCAQDISFVQEM--IS-RLEQTDYKLKLCVFDRDVLPGTCLWSITSELIEHRCRKMVVIISDDYLDSSECDFQTKFALSLGPGAREKRLIPVKYKPMKRP--FPSILRFITLCDYTNPCTKGWFWERLAKAL 281          
BLAST of Myeloid differentiation primary response protein MyD88 vs. TrEMBL
Match: A0A0A7NVN0 (MYD88 OS=Cyclina sinensis OX=120566 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.405e-16
Identity = 78/300 (26.00%), Postives = 139/300 (46.33%), Query Frame = 3
Query:  276 NISDYFSSVPIHALSEKCKEKLKF-LDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKME---KRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVN-QEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRGLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFDSFINSF 1160
            ++S  +S +P+ AL+  C+ ++   L+    + +  + + DYRGLA L+ ++ LE+      +R  SPT  +L           T   L+  L  L+R D++ + ++ + RD  ++L+   +F    +  Q+     T   ++DE+      LF   + +  + +  IYD F+ +  E  D   +  LI       GL +       + G   N   A  I  R K++I+++S +++ S +C  Q  FA      SR  K+IPV IE+   PE I  +   NY    +RE F+   I+S 
Sbjct:   10 DLSSKYSVIPLEALNASCRRQIALHLNLEGTMNEDNI-INDYRGLAELVGFNYLEIKNF---ERNNSPTEQLLSDWTTRTDLSPTVGTLWKNLLSLERVDVLTDSKKYIERDCEKYLEIKARFQTSQIPVQDNTVSQTPDHDVDES------LFM-GVDDVIHGKPTIYDAFVCYNPEGKDLAFVRQLIQKLEVENGLKLFVPWRDDLPGASHNTVCAKLIEYRCKRMIIILSPSYLQSPACDFQTKFAHGLSPGSRNKKLIPVLIEDCVIPEIIRHVTCCNYTKKDLREWFWSRLISSL 298          
BLAST of Myeloid differentiation primary response protein MyD88 vs. TrEMBL
Match: A0A3G1HV23 (Myeloid differentiation factor 88 OS=Anadara sativa OX=1028096 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.518e-15
Identity = 75/298 (25.17%), Postives = 143/298 (47.99%), Query Frame = 3
Query:  273 MNISDYFSSVPIHALSEKCKEKL-KFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKME---KRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEH--VNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESR-GLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIE-EVKKPECIASINGFNYYNNSIREMFFD 1142
            M++SD +  +P+  L+   + KL K+L+      +    + DY GLA L+ +  L++      +R  SPT  +LD        K T  +L+  L +L R D++++C + + RD   FL++  KF  E+  V ++   Q T         +T+  + + D +E     +  YD F+ +  E   D+K +  +   LE++  L +       + G       A  I +R +++++++S+ ++ S +C  Q+ FA      +R  K+IPV IE  VK P+ +  +   ++    + E F+D
Sbjct:   14 MSLSDQYKEIPLDGLNISSRRKLAKYLNIPAEFHEDSGHIGDYSGLAELIGFDYLDIKNF---ERQKSPTEEVLDAWSQRADFKPTIGNLWKYLIELGRLDVLEDCEQSIRRDIEGFLQRRAKFQDEYKPVQEDTVSQSTETAPDHHVNETL--ILSKDDVEG---HLQFYDAFVCYNPEG-GDVKFVKEMIKMLENKYKLKLFVPYRDDLPGGARYVMDAKLIESRCRRMVIIMSQNYMKSSACDFQVKFAHALSPGARSKKLIPVLIESSVKIPQILRHVTLCDFTKQDLMEWFWD 302          
BLAST of Myeloid differentiation primary response protein MyD88 vs. TrEMBL
Match: A0A5C0NSG4 (Myeloid differentiation factor 88 OS=Mimachlamys nobilis OX=106276 GN=Myd88 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 4.445e-15
Identity = 75/312 (24.04%), Postives = 143/312 (45.83%), Query Frame = 3
Query:  300 VPIHALSEKCKEKLKFL--DCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRM---KMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESR--GLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFDSFINSF*TVLHGMSRTILYDFLSY 1214
            V + +L+  C+ K+  L      +IPD+++   D+ G A LLN+S  E+  L   KR+ SPT  +L      K  + T  +L   L +L+R D+I +CR  + RD  ++ K+  K + +  N   F+       L+       +     + E        YD F+ +  ++ D +  +  +SG LE     L +      ++ G  ++   A  I  + ++  +++SK+F NS +   QL FA      +R+ ++IP+ IE+++ P  +  +    +YN  IR   +     +  + L     T L ++ ++
Sbjct:   17 VKLKSLNNGCRRKIGMLLDPEGSLIPDSDM-FNDWGGYAELLNFSQPEIENL---KRHKSPTQEMLHLWTTRKYPEPTVGNLIRFLYQLERVDVITDCRNMIERDVKKW-KENEKSIEDMYNDPTFRD----PNLNATSNPPSRKTPESVPEVEREHEMYYDCFVIYNPDAPDQLDFVKEMSGILEGPEYNLRLFIPWRDYLVGTAMHSVSAAIIEKKCRRCAVILSKSFNNSPAADFQLKFAHALSPGARQKRVIPIVIEKMEVPNILNFVAPAPFYNVGIRHWQWPRVAATIKSELLPDPETWLPEYDAW 319          
BLAST of Myeloid differentiation primary response protein MyD88 vs. TrEMBL
Match: A0A0H3Y827 (MyD88-1 OS=Mizuhopecten yessoensis OX=6573 PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 7.267e-15
Identity = 75/298 (25.17%), Postives = 140/298 (46.98%), Query Frame = 3
Query:  276 NISDYFSSVPIHALSEKCKEKLK-FLDC-TFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQI-TSPTELDEAKKTIGKLFTTDIIETANRQIDIY-DVFISFESESIDDIKIMWLISGRLESRGLTVAKGSYSF---------IQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIRE 1130
            N+ +    V +++L+  C+ K+  F+D    +IPD+++   D+ G A LLN+S  E+  +  QK  T     +       + T  +L   L +L+R D+I  CR+ + RD  ++ +          NQ++ K+I   PT  D +  +       + +  A  Q +IY D F+ +  ++ D ++ +  +S  LE          Y+F         + G  ++   A  I  + ++ I+++SK+F NS +   QL FA      +R+ ++IP+ IE V+ P  +  +    +YN  IR 
Sbjct:    8 NLIESMKDVHMNSLNNGCRRKIGMFMDPEGSLIPDSDM-FNDWCGCAELLNFSQPEIENMKRQKSPTQEMLHLWSTRNDPEPTVGNLISFLFQLERFDVISECRQMIERDVKKWKQ----------NQQSLKKIYDDPTFRDPSSPSSPPPDKIETVSDAGSQHEIYYDCFVIYNPDASDQLEFVKEMSRILEG-------PEYNFRLFIPWRDDLVGTAMHSVSAAIIEKKCRRCIVIVSKSFNNSPAADFQLKFAHALSPGARQKRVIPIVIENVEVPSILNFVAPAPFYNVGIRH 287          
BLAST of Myeloid differentiation primary response protein MyD88 vs. TrEMBL
Match: A0A2U6KF89 (Myeloid differentiation factor 88 OS=Pinctada fucata OX=50426 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 2.021e-14
Identity = 76/297 (25.59%), Postives = 135/297 (45.45%), Query Frame = 3
Query:  276 NISDYFSSVPIHALSEKCKEKL-KFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRM--KMEKRTFLD-LYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQ----ID--IYDVFISFESESIDDIKIMWLISGRLESR--GLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIRE 1130
            +I D    VP+HAL+   + K+  FLD    +  T     DY GLA    +   E+  L  Q++   PT   L     + ++  ++D L   L+++ R D+ID CR+++ +D  ++     K   E ++    K+++   E+   K  I     T       RQ    ID   YD F+ +      D+  +  ++ RLE    GL +       + G    E  A  I NR K+ ++++S +F+ S +   QL FA+     +R  K++PV+    K P  + ++N  ++ N  +R+
Sbjct:    6 SIKDVCKGVPLHALNMAVRNKMASFLDPEGFV--TGDFCNDYTGLAECAGFQYEEIKRLQSQRK---PTLEFLHEWGTRQDRDPYVDVLISHLERIGRPDVIDECRQQIVQDVQKY-----KRCHEDLDPVQSKEVSCGNEVTLYKHEIKSSILTKEEALDPRQACGSIDKLYYDGFVIYNPNGQADLNFVKELADRLEGPEYGLKLFIPFRDDLPGTARYEVTAEIIANRCKRALVILSPSFLQSPAADFQLKFAQCLSPGARSKKVVPVFNGRCKMPNILRAVNFVDFTNPGLRD 292          
BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Nematostella
Match: EDO37422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFW6])

HSP 1 Score: 45.4394 bits (106), Expect = 3.982e-6
Identity = 35/123 (28.46%), Postives = 60/123 (48.78%), Query Frame = 3
Query:  774 FISFESESIDDIKIMWLISGRLESRGLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFD 1142
            FI + S+   DIK    +  RLE+ G         F+ G  I    ANAIY+ S+K + ++S  FI SE C  +L+ A   +R+ R ++++ +   E   P  +      +Y N  ++  F++
Sbjct:    1 FICYSSK---DIKFAKELQERLENAGFKCCIDLRDFMPGEAIVVNIANAIYH-SRKTLAILSPDFIESEYCKHELHQA--LNRSIRAHQVVAILYRECNVPLELRHKTYLDYLNCYVKPHFWE 117          
BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Medaka
Match: myd88 (MYD88 innate immune signal transduction adaptor [Source:NCBI gene;Acc:101168580])

HSP 1 Score: 53.1434 bits (126), Expect = 2.150e-7
Identity = 56/265 (21.13%), Postives = 118/265 (44.53%), Query Frame = 3
Query:  297 SVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLEL-NYLIDQKRYTSPTYLILDRMKMEKR--TFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLES--RGLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKP 1076
            +VP+ AL+   + KL      ++ P   V   D+  +A  + ++ LE+ NY    +   +PT  +L+  +   +  +   L   L +++R D++++ R ++  D  ++L+   +        E   Q+    E+D       + F   + +  +   +++D FI +     DD   +  +   LE     L +       + G C+    +  I  R K+++++IS  ++ S++C  Q  FA      +R  ++IPV  + +KKP
Sbjct:   12 TVPLVALNVTVRRKLGL----YLNPKNTVAA-DWMTVAEEMGFTYLEIKNY----EASKNPTKTVLEDWQARSKDASVGKLLSILTEVERKDVVEDLRPQIDEDVRKYLESLQR------KSEPPLQVA---EVDSCVPRTPERFGITVEDDPDGSPEMFDAFICY---CQDDFHFVCEMIRELEQTDHKLKLCVFDRDVLPGSCVWTITSELIEKRCKRMVVVISDEYLESDACDFQTKFALSLCPGARNKRLIPVIYKTMKKP 255          
BLAST of Myeloid differentiation primary response protein MyD88 vs. Planmine SMEST
Match: SMESG000051766.1 (SMESG000051766.1)

HSP 1 Score: 601.282 bits (1549), Expect = 0.000e+0
Identity = 295/296 (99.66%), Postives = 296/296 (100.00%), Query Frame = 3
Query:  273 MNISDYFSSVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRGLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFDSFINSF 1160
            MNISDYFSSVPIHALSEKCKEKLKFLDCTFIIPDTE+KLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRGLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFDSFINSF
Sbjct:    1 MNISDYFSSVPIHALSEKCKEKLKFLDCTFIIPDTEMKLRDYRGLAVLLNYSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIIDNCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLFTTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRGLTVAKGSYSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADRASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFDSFINSF 296          
The following BLAST results are available for this feature:
BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
myd882.970e-821.76MYD88 innate immune signal transduction adaptor [S... [more]
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
myd881.264e-622.48myeloid differentiation primary response 88 [Sourc... [more]
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9DF60|MY88A_XENLA5.516e-1123.31Myeloid differentiation primary response protein M... [more]
sp|Q5FWM2|MY88B_XENLA3.935e-824.54Myeloid differentiation primary response protein M... [more]
sp|Q5XJ85|MYD88_DANRE2.764e-721.76Myeloid differentiation primary response protein M... [more]
sp|Q28DJ2|MYD88_XENTR5.688e-622.48Myeloid differentiation primary response protein M... [more]
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BLAST of Myeloid differentiation primary response protein MyD88 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0A7NVN01.405e-1626.00MYD88 OS=Cyclina sinensis OX=120566 PE=2 SV=1[more]
A0A3G1HV231.518e-1525.17Myeloid differentiation factor 88 OS=Anadara sativ... [more]
A0A5C0NSG44.445e-1524.04Myeloid differentiation factor 88 OS=Mimachlamys n... [more]
A0A0H3Y8277.267e-1525.17MyD88-1 OS=Mizuhopecten yessoensis OX=6573 PE=2 SV... [more]
A0A2U6KF892.021e-1425.59Myeloid differentiation factor 88 OS=Pinctada fuca... [more]
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO374223.982e-628.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
myd882.150e-721.13MYD88 innate immune signal transduction adaptor [S... [more]
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BLAST of Myeloid differentiation primary response protein MyD88 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000051766.10.000e+099.66SMESG000051766.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014235 ID=SMED30014235|Name=Myeloid differentiation primary response protein MyD88|organism=Schmidtea mediterranea sexual|type=transcript|length=1401bp
CATTTTTCGATGGAAATTTGTTTTCTTTCGGATCACAGACAAGAGGATTT
TGATAAAGTTTGATAAAAAATAAAACTAATTTTCCAACTTACTTTATTAT
ACATAATTTTCTCTGTTGATTTTTAATATTGATTTTCTAATAATCATTAT
AACGGCATCCAATCAAAGTCCATGAAAAATCGATCGATTGTCAGTGATTT
GCTAATCATATGTATTAAGCGTCAATCCATCAATTGCACTGCATTCATCG
GCAGATAATAACAATAATATTCATGAATATTTCTGATTACTTTTCTTCAG
TTCCAATTCATGCTTTGTCAGAAAAATGCAAAGAAAAACTGAAATTTCTC
GACTGTACCTTTATCATTCCTGACACGGAAGTGAAACTTCGGGATTACAG
AGGATTGGCGGTTTTGTTGAATTATTCCACTTTGGAACTGAATTATTTAA
TCGACCAAAAACGATACACAAGTCCGACCTATTTGATTCTGGATAGAATG
AAAATGGAAAAGAGGACGTTTCTTGATTTGTATTGTTGTTTGAAAAAATT
GAAACGAAGTGACATAATTGACAACTGCAGAGAAGAATTGACAAGAGACG
CTATGGAATTTTTGAAAAAAACTGGCAAATTTCTATTCGAGCACGTGAAT
CAGGAAGCATTTAAACAAATCACATCACCAACGGAACTCGATGAAGCGAA
AAAGACAATTGGGAAATTATTCACCACAGACATAATCGAAACCGCTAATA
GACAAATTGACATTTATGATGTTTTTATTAGTTTTGAATCGGAATCCATT
GATGATATCAAGATAATGTGGTTGATTAGCGGTAGGCTGGAAAGCAGGGG
TCTGACGGTAGCAAAGGGGTCGTACTCATTTATTCAAGGAGTCTGTATTA
ATGAGGCATTTGCCAATGCCATTTATAATCGAAGCAAGAAAGTTATTCTG
CTCATTAGTAAGGCCTTTATTAATTCTGAGAGCTGTCTGATGCAACTGAA
TTTTGCGAGAGATGCTGATAGGGCGTCAAGGAGAAACAAGATTATTCCAG
TTTATATTGAAGAAGTTAAGAAACCAGAATGCATTGCTAGTATTAACGGA
TTTAACTATTATAACAATTCCATCCGTGAAATGTTTTTTGATAGTTTTAT
TAATTCTTTTTAAACTGTCCTTCATGGCATGAGTAGAACCATTTTGTACG
ACTTTCTATCATATTTGTTAAATATTATTTTGATACTTCTTTTAATTATT
ATTTGTTTTAACCATTCGTTGATTAAAGTACTTTGTAAACATCTGTAATC
ATTCCAAGTGCTATATGTTTATGTTTATGTTAATAATGATATGTGTATCA
GTGCCATGTTTGTAATTTTGACGTCCCTAAATAATGCATATTGAAGCGCC
C
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protein sequence of SMED30014235-orf-1

>SMED30014235-orf-1 ID=SMED30014235-orf-1|Name=SMED30014235-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=297bp
MNISDYFSSVPIHALSEKCKEKLKFLDCTFIIPDTEVKLRDYRGLAVLLN
YSTLELNYLIDQKRYTSPTYLILDRMKMEKRTFLDLYCCLKKLKRSDIID
NCREELTRDAMEFLKKTGKFLFEHVNQEAFKQITSPTELDEAKKTIGKLF
TTDIIETANRQIDIYDVFISFESESIDDIKIMWLISGRLESRGLTVAKGS
YSFIQGVCINEAFANAIYNRSKKVILLISKAFINSESCLMQLNFARDADR
ASRRNKIIPVYIEEVKKPECIASINGFNYYNNSIREMFFDSFINSF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR035897Toll_tir_struct_dom_sf
IPR011029DEATH-like_dom_sf
IPR000488Death_domain
IPR000157TIR_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0070976TIR domain binding
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
GO:0002376immune system process
GO:0002755MyD88-dependent toll-like receptor signaling pathway
GO:0006954inflammatory response
GO:0043123positive regulation of I-kappaB kinase/NF-kappaB signaling
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilyGENE3DG3DSA:3.40.50.10140coord: 165..296
e-value: 4.7E-16
score: 60.9
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilySUPERFAMILYSSF52200Toll/Interleukin receptor TIR domaincoord: 164..270
NoneNo IPR availableGENE3DG3DSA:1.10.533.10coord: 9..125
e-value: 1.6E-13
score: 52.5
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPFAMPF13676TIR_2coord: 167..279
e-value: 6.8E-12
score: 46.0
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPROSITEPS50104TIRcoord: 163..267
score: 13.676
IPR000488Death domainPFAMPF00531Deathcoord: 37..104
e-value: 6.7E-5
score: 23.0
IPR011029Death-like domain superfamilySUPERFAMILYSSF47986DEATH domaincoord: 35..103