SMED30014233

Overview
NameSMED30014233
Smed IDSMED30014233
Length (bp)777
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014233 (SMED30014233) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014233 (SMED30014233) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014233 (SMED30014233) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014233 (SMED30014233) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014233

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30014233SMESG000045992.1 SmedASXL_007986SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014233SMESG000045992.1 Contig41550newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014233SMESG000045992.1 Contig41550uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30014233SMESG000045992.1 dd_Smed_v6_6974_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30014233SMESG000045992.1 dd_Smed_v4_6974_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014233 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003914:42262..43084 -3803
v31.002375:44669..45491 +3785
Homology
[Close]
BLAST of SMED30014233 vs. Ensembl Human
Match: CCDC153 (coiled-coil domain containing 153 [Source:HGNC Symbol;Acc:HGNC:27446])

HSP 1 Score: 51.2174 bits (121), Expect = 3.130e-7
Identity = 44/159 (27.67%), Postives = 79/159 (49.69%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGIT 671
            L++HL  RR  AR+A  SE  L + LQ   + +LE   S+ K + A M++Q   ++  +     +L  E+   + +LE+     +   +E E+   E+D  +  L+  +  +  +Y+ I++  LD+L   L   K QW    + LH+  K+    FG+T
Sbjct:   41 LRDHLALRRDEARRAKASEDQLRQRLQG-VEAELEGARSEGKAIYAEMSRQCHALQEDMQTRSKQLEEEVKGLRGQLEACQREAAAAREEAEQALGERDQALAQLRAHMADMEAKYEEILHDSLDRLLAKLRAIKQQWDGAALRLHARHKEQQRQFGLT 198
[Close]
BLAST of SMED30014233 vs. Ensembl Human
Match: CCDC153 (coiled-coil domain containing 153 [Source:HGNC Symbol;Acc:HGNC:27446])

HSP 1 Score: 51.2174 bits (121), Expect = 3.130e-7
Identity = 44/159 (27.67%), Postives = 79/159 (49.69%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGIT 671
            L++HL  RR  AR+A  SE  L + LQ   + +LE   S+ K + A M++Q   ++  +     +L  E+   + +LE+     +   +E E+   E+D  +  L+  +  +  +Y+ I++  LD+L   L   K QW    + LH+  K+    FG+T
Sbjct:   41 LRDHLALRRDEARRAKASEDQLRQRLQG-VEAELEGARSEGKAIYAEMSRQCHALQEDMQTRSKQLEEEVKGLRGQLEACQREAAAAREEAEQALGERDQALAQLRAHMADMEAKYEEILHDSLDRLLAKLRAIKQQWDGAALRLHARHKEQQRQFGLT 198
[Close]
BLAST of SMED30014233 vs. Ensembl Zebrafish
Match: ccdc153 (coiled-coil domain containing 153 [Source:NCBI gene;Acc:792470])

HSP 1 Score: 65.855 bits (159), Expect = 1.367e-12
Identity = 51/172 (29.65%), Postives = 98/172 (56.98%), Query Frame = 3 
Query:  162 QKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDL--QHRTQQQLESFE-SDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            +K+ +   +V  L+ HL  R   AR+A + R   ++L  Q R  +QL S E SD K++ A + +QYK M+  L ++ D+L   ++  + +L    + L    + +E    EKD  I++L+++++S+ ++ + I++G LD L +HL   + +W+ ++  +H + K  L +FGI
Sbjct:   29 EKYRRSVLDVSVLKEHLALRSGVARQATAVR---DELKSQVRDLEQLLSQERSDMKDITADLNRQYKSMETDLQSKADKLEASVDLLEKQLAECQVELKSERELRENTEAEKDAIISDLQSKLDSMERECEKILHGCLDSLLSHLADTRMKWEEQSTVIHQDVKDMLREFGI 197
[Close]
BLAST of SMED30014233 vs. Ensembl Mouse
Match: Ccdc153 (coiled-coil domain containing 153 [Source:MGI Symbol;Acc:MGI:2448587])

HSP 1 Score: 51.9878 bits (123), Expect = 1.040e-7
Identity = 47/158 (29.75%), Postives = 79/158 (50.00%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            LQ+ L  +R  AR+A  SE  L + LQ   + +LE  +S+ K + A M++Q + +K  LG    +L  E+   K +LE+         +E E+  R++D  +  L   +  +  +Y+ I++  LD L   L V K  W    + LH+  K+ L  FG+
Sbjct:   41 LQDRLALQREEARRAKASEDRLKQRLQG-LEAELERTQSEGKAIYAEMSRQRQALKEELGTRSKQLEEEVRSLKEQLETCQREAKTAKEEAERALRKQDGTLAQLHAHVADMEAKYEEILHDNLDCLLAKLRVVKPHWDANVLRLHTRLKEQLRQFGL 197
[Close]
BLAST of SMED30014233 vs. Ensembl Mouse
Match: Ccdc153 (coiled-coil domain containing 153 [Source:MGI Symbol;Acc:MGI:2448587])

HSP 1 Score: 46.595 bits (109), Expect = 5.209e-6
Identity = 48/165 (29.09%), Postives = 80/165 (48.48%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIIN---GV----LDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            LQ+ L  +R  AR+A  SE  L + LQ   + +LE  +S+ K + A M++Q + +K  LG    +L  E+   K +LE+         +E E+  R++D  +  L   +  +  +Y+ I++   G     LD L   L V K  W    + LH+  K+ L  FG+
Sbjct:    4 LQDRLALQREEARRAKASEDRLKQRLQG-LEAELERTQSEGKAIYAEMSRQRQALKEELGTRSKQLEEEVRSLKEQLETCQREAKTAKEEAERALRKQDGTLAQLHAHVADMEAKYEEILHLKAGCSQDNLDCLLAKLRVVKPHWDANVLRLHTRLKEQLRQFGL 167
[Close]
BLAST of SMED30014233 vs. Ensembl Mouse
Match: Ccdc153 (coiled-coil domain containing 153 [Source:MGI Symbol;Acc:MGI:2448587])

HSP 1 Score: 46.595 bits (109), Expect = 7.732e-6
Identity = 48/165 (29.09%), Postives = 80/165 (48.48%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIIN---GV----LDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            LQ+ L  +R  AR+A  SE  L + LQ   + +LE  +S+ K + A M++Q + +K  LG    +L  E+   K +LE+         +E E+  R++D  +  L   +  +  +Y+ I++   G     LD L   L V K  W    + LH+  K+ L  FG+
Sbjct:   41 LQDRLALQREEARRAKASEDRLKQRLQG-LEAELERTQSEGKAIYAEMSRQRQALKEELGTRSKQLEEEVRSLKEQLETCQREAKTAKEEAERALRKQDGTLAQLHAHVADMEAKYEEILHLKAGCSQDNLDCLLAKLRVVKPHWDANVLRLHTRLKEQLRQFGL 204
[Close]
BLAST of SMED30014233 vs. UniProt/SwissProt
Match: sp|A8KB59|CC153_DANRE (Coiled-coil domain-containing protein 153 OS=Danio rerio OX=7955 GN=ccdc153 PE=2 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.032e-11
Identity = 51/172 (29.65%), Postives = 98/172 (56.98%), Query Frame = 3 
Query:  162 QKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDL--QHRTQQQLESFE-SDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            +K+ +   +V  L+ HL  R   AR+A + R   ++L  Q R  +QL S E SD K++ A + +QYK M+  L ++ D+L   ++  + +L    + L    + +E    EKD  I++L+++++S+ ++ + I++G LD L +HL   + +W+ ++  +H + K  L +FGI
Sbjct:   29 EKYRRSVLDVSVLKEHLALRSGVARQATAVR---DELKSQVRDLEQLLSQERSDMKDITADLNRQYKSMETDLQSKADKLEASVDLLEKQLAECQVELKSERELRENTEAEKDAIISDLQSKLDSMERECEKILHGCLDSLLSHLADTRMKWEEQSTVIHQDVKDMLREFGI 197
[Close]
BLAST of SMED30014233 vs. UniProt/SwissProt
Match: sp|Q5FVL4|CC153_RAT (Coiled-coil domain-containing protein 153 OS=Rattus norvegicus OX=10116 GN=Ccdc153 PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.652e-7
Identity = 46/158 (29.11%), Postives = 78/158 (49.37%), Query Frame = 3 
Query:  198 LQNHL-IQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            LQ+HL +QR    R   SE  L + LQ   + +LE  +S+ + V A M++Q + ++  LG     L  E+   +  LE+         +E E+  RE+D  +  L+  +  +  +Y+ I++  L+ L   L   K QW   T+ LH+  K+ L  FG+
Sbjct:   41 LQDHLALQREEACRAKASEDRLKQRLQG-LEAELERTQSEGRAVYAEMSRQRQALQKELGTRSKRLEEEVRGLRERLETCQREAKTAREEAERALREQDGTLTELRAHVAHMEAKYEEILHDNLNCLLAKLRAVKPQWDAATLRLHTRHKEQLRRFGL 197
[Close]
BLAST of SMED30014233 vs. UniProt/SwissProt
Match: sp|P0C7Q1|CC153_MOUSE (Coiled-coil domain-containing protein 153 OS=Mus musculus OX=10090 GN=Ccdc153 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 7.277e-7
Identity = 47/158 (29.75%), Postives = 79/158 (50.00%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            LQ+ L  +R  AR+A  SE  L + LQ   + +LE  +S+ K + A M++Q + +K  LG    +L  E+   K +LE+         +E E+  R++D  +  L   +  +  +Y+ I++  LD L   L V K  W    + LH+  K+ L  FG+
Sbjct:   41 LQDRLALQREEARRAKASEDRLKQRLQG-LEAELERTQSEGKAIYAEMSRQRQALKEELGTRSKQLEEEVRSLKEQLETCQREAKTAKEEAERALRKQDGTLAQLHAHVADMEAKYEEILHDNLDCLLAKLRVVKPHWDANVLRLHTRLKEQLRQFGL 197
[Close]
BLAST of SMED30014233 vs. UniProt/SwissProt
Match: sp|Q494R4|CC153_HUMAN (Coiled-coil domain-containing protein 153 OS=Homo sapiens OX=9606 GN=CCDC153 PE=1 SV=2)

HSP 1 Score: 51.2174 bits (121), Expect = 1.503e-6
Identity = 44/159 (27.67%), Postives = 79/159 (49.69%), Query Frame = 3 
Query:  198 LQNHLIQRRAYARKAN-SERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGIT 671
            L++HL  RR  AR+A  SE  L + LQ   + +LE   S+ K + A M++Q   ++  +     +L  E+   + +LE+     +   +E E+   E+D  +  L+  +  +  +Y+ I++  LD+L   L   K QW    + LH+  K+    FG+T
Sbjct:   41 LRDHLALRRDEARRAKASEDQLRQRLQG-VEAELEGARSEGKAIYAEMSRQCHALQEDMQTRSKQLEEEVKGLRGQLEACQREAAAAREEAEQALGERDQALAQLRAHMADMEAKYEEILHDSLDRLLAKLRAIKQQWDGAALRLHARHKEQQRQFGLT 198
[Close]
BLAST of SMED30014233 vs. TrEMBL
Match: A0A3S1B1H2 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_014654 PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 8.503e-22
Identity = 56/173 (32.37%), Postives = 104/173 (60.12%), Query Frame = 3 
Query:  150 LDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            L++E KF K  +E+  L++HL  RR + R++ S        +   +++LE  + D + +NA MT+QYK M+  +G  I +L  EL +T+ +L ST   L ++ ++++K+  ++D+EI +L+ +I+S+ K Y+ ++   LD L   L   K+ W+ R+  +   +K+ LLDFG+
Sbjct:   24 LELEDKFKKTMDEIEALKDHLAIRREFTRRSKSASAEMARKKQEAEKELEEHKEDQRAINADMTRQYKTMQTEMGLRIHQLETELGRTRSQLSSTEAELRKSYEDRKKMIEQRDSEIMDLRLKIDSMEKAYENVLREALDNLMKKLDDAKELWEGRSAIIQGKNKQTLLDFGL 196
[Close]
BLAST of SMED30014233 vs. TrEMBL
Match: A0A0B6YRD7 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF33941 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 2.910e-20
Identity = 58/174 (33.33%), Postives = 106/174 (60.92%), Query Frame = 3 
Query:  150 LDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGIT 671
            L+++ K+ +  +E+  L++HL  RR   R++ S R         T+++L+  + + K++NA MT+QYK M+  +G  I  L  EL +T+ +L ST   L ++ +EK K+  E+D EI +LK +I+S+ + Y+++I   LD L   L   KD+W+++   +   +K+ALL+FG++
Sbjct:   39 LELDDKYRRTVDEITALKDHLAIRRDLTRRSKSARAEVSQKLEDTEKELQEHKENQKDINADMTRQYKTMQTEMGLRIHHLESELRQTQKQLSSTETELKESYEEKAKVMEERDAEIVDLKLKIDSMEQAYESVIREALDSLMRKLDDSKDKWESKASLIQVKNKQALLEFGLS 212
[Close]
BLAST of SMED30014233 vs. TrEMBL
Match: A0A2T7PGB9 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_07917 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.149e-18
Identity = 54/173 (31.21%), Postives = 99/173 (57.23%), Query Frame = 3 
Query:  150 LDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            L++E K+ +  +E+  L++HL  R    R+A S      +   ++++ L+    D K ++A MT+QYK M+  +G  I +L  EL KT+  LE     L Q  +EK++  +++D EI+ LK ++ S+ K Y+AI+   LD L   + +  D W+ ++  +   +K+A+L+FG+
Sbjct:   22 LELEDKYKRTMDEIEALKDHLALRSELTRRAQSASAQVYERMTQSEKMLQEQIDDQKAISADMTRQYKTMQTEMGLRIHQLETELGKTQAHLEHAKFELQQAREEKDRSVKDRDEEISELKMKVSSMEKAYEAILREALDGLMAKIDLAHDNWEVKSTNIQGKTKQAVLEFGL 194
[Close]
BLAST of SMED30014233 vs. TrEMBL
Match: A0A2C9LSQ4 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106056313 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.745e-18
Identity = 59/178 (33.15%), Postives = 101/178 (56.74%), Query Frame = 3 
Query:  150 LDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGITHQFI 683
            L++E  + +  +E+  L++HL  RR   R+A S           T++ L+  + D K++NA MT+QYK M+  +G  I +L  EL +T+ +L +T   L ++ ++++K+  EKDN I +LK +I+S+ K YD ++   LD L   L   K  W   +  + S +K+ LL+FG+  Q I
Sbjct:   22 LELEDSYKRTLDEIAALKDHLAVRRDLTRRAQSASLDITKRMEETERLLKEHKEDQKDINADMTRQYKTMQTEMGLRIHQLETELGRTRTQLSATEAELKKSYEDRKKMIDEKDNIIKDLKLKIDSMEKAYDNVLREALDNLIIKLDAAKLLWDQHSSLIQSKNKQTLLEFGLNPQDI 199
[Close]
BLAST of SMED30014233 vs. TrEMBL
Match: V4AHA5 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_224434 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 8.265e-18
Identity = 58/176 (32.95%), Postives = 109/176 (61.93%), Query Frame = 3 
Query:  150 LDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHR---TQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            L++E K+ +  +E+  L++HL  R+ + R++ + +   ++++ R    +++LE  +S+ K VNA MT+QYK M+  +   I +L  EL +T+  L  T + L +T +EK+KI + ++  I++LK +I S+ K Y+ I+ G LD L   + V K++W+ R+  + S +K  LL+FG+
Sbjct:   24 LELEDKYKRTVDEIQALKDHLSIRKEFTRRSVAYK---DEMRQRMLGAEEELEDTKSNQKTVNADMTRQYKTMQTEMSLRIHQLETELARTRSGLSQTELELKKTREEKDKIIQNRELTISDLKLKINSMEKDYEGILRGALDALIQKVEVAKERWEERSTTIQSKNKNVLLEFGL 196
[Close]
BLAST of SMED30014233 vs. Ensembl Cavefish
Match: ccdc153 (coiled-coil domain-containing protein 153-like [Source:NCBI gene;Acc:111193708])

HSP 1 Score: 50.447 bits (119), Expect = 2.753e-7
Identity = 42/180 (23.33%), Postives = 97/180 (53.89%), Query Frame = 3 
Query:  153 DIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQ---QQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGITHQFI 683
            ++ Q + +   EV  L++ L  +   A++A S R   +DLQ++ +   Q L   +   K++   + +QYK ++  L A+++ L   +++ + +L+    +L    +++E+   EKD +I +L+ +++++  +Y+ I++G LD L +HL   +  W+  +  +H   K  L++  +   +I
Sbjct:   25 EVAQSYRRSLLEVAVLKDRLALKTDVAQQAISAR---DDLQYQIRDLKQTLSQEKLGMKDITTDLNRQYKTLQTNLEAKVEYLETRVSELQQQLDQCQGDLKTEREQREQTEEEKDAQITDLQIKLDNMETEYEKILHGCLDSLLSHLAEARRNWEDVSSDIHQEFKDMLIELRLNPHYI 201
[Close]
BLAST of SMED30014233 vs. Ensembl Nematostella
Match: EDO33635 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRQ0])

HSP 1 Score: 46.595 bits (109), Expect = 1.428e-6
Identity = 35/111 (31.53%), Postives = 65/111 (58.56%), Query Frame = 3 
Query:  336 MTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGI 668
            +T+QYK M+  +   I  L   + K + +L ST   L +T KE++ + REKD  I+ LK +IE +   Y++I++   DK+   +   + +W+T++  +   +K+ LL+FG+
Sbjct:   47 LTRQYKTMQSEMALRIHMLEQTVTKLREQLASTEEELQKTRKERDDMRREKDQIISELKFKIEHMESVYESILHDAFDKMTLKVDEARSKWETQSSEIQERNKQILLEFGL 157
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BLAST of SMED30014233 vs. Planmine SMEST
Match: SMESG000045992.1 (SMESG000045992.1)

HSP 1 Score: 336.65 bits (862), Expect = 3.069e-118
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 3 
Query:   72 MPPXXXXXXXXXXXXXXXXXXXXREDLDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGITHQFI 683
            MPPKKKKGKKGGKKKGGKKSGKKREDLDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQTIKEKEKITREKDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGITHQFI
Sbjct:    1 MPPKKKKGKKGGKKKGGKKSGKKREDLDIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQTIKEKEKITREKDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGITHQFI 204
[Close]
BLAST of SMED30014233 vs. Planmine SMEST
Match: SMESG000046943.1 (SMESG000046943.1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.297e-8
Identity = 44/178 (24.72%), Postives = 90/178 (50.56%), Query Frame = 3 
Query:  153 DIEQKFSKIENEVVNLQNHLIQRRAYARKANSERFLAEDLQHR-TQQQLESFESDSKEVNAFMTKQYKIMKITLGAEIDELTLELNKTKIELESTSINLSQXXXXXXXXXXXXDNEINNLKTEIESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGITHQFI 683
            D+     K+ N++ +L+    QR  YARK N E+FL   L +   + ++ + E D  + ++ +  QYK ++      + EL  +L++   EL  T I + + I  ++   +   + I +L+  I ++H ++  ++     +L+  + VD D W+   + LH ++K+ LL++ I    I
Sbjct:   18 DLNMALQKMTNQMNSLKLVFNQRSMYARK-NHEKFLEATLYNEELRLEINAMEEDFHQFSSCINLQYKFLQKLTVLRVKELDEKLHQVVKELHLTKIKMKEEIAYRDNNIKLMADRILDLEMHINTMHLKWQKVLMESFGELERRVSVDWDTWKLGHLKLHKSTKQKLLEYSICFNVI 194
The following BLAST results are available for this feature:
BLAST of SMED30014233 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CCDC1533.130e-727.67coiled-coil domain containing 153 [Source:HGNC Sym... [more]
CCDC1533.130e-727.67coiled-coil domain containing 153 [Source:HGNC Sym... [more]
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BLAST of SMED30014233 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014233 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014233 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc1531.367e-1229.65coiled-coil domain containing 153 [Source:NCBI gen... [more]
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BLAST of SMED30014233 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014233 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Ccdc1531.040e-729.75coiled-coil domain containing 153 [Source:MGI Symb... [more]
Ccdc1535.209e-629.09coiled-coil domain containing 153 [Source:MGI Symb... [more]
Ccdc1537.732e-629.09coiled-coil domain containing 153 [Source:MGI Symb... [more]
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BLAST of SMED30014233 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|A8KB59|CC153_DANRE1.032e-1129.65Coiled-coil domain-containing protein 153 OS=Danio... [more]
sp|Q5FVL4|CC153_RAT2.652e-729.11Coiled-coil domain-containing protein 153 OS=Rattu... [more]
sp|P0C7Q1|CC153_MOUSE7.277e-729.75Coiled-coil domain-containing protein 153 OS=Mus m... [more]
sp|Q494R4|CC153_HUMAN1.503e-627.67Coiled-coil domain-containing protein 153 OS=Homo ... [more]
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BLAST of SMED30014233 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S1B1H28.503e-2232.37Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A0B6YRD72.910e-2033.33Uncharacterized protein (Fragment) OS=Arion vulgar... [more]
A0A2T7PGB91.149e-1831.21Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A2C9LSQ41.745e-1833.15Uncharacterized protein OS=Biomphalaria glabrata O... [more]
V4AHA58.265e-1832.95Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of SMED30014233 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ccdc1532.753e-723.33coiled-coil domain-containing protein 153-like [So... [more]
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BLAST of SMED30014233 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014233 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014233 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO336351.428e-631.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30014233 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014233 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000045992.13.069e-118100.00SMESG000045992.1[more]
SMESG000046943.11.297e-824.72SMESG000046943.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014233 ID=SMED30014233|Name=SMED30014233|organism=Schmidtea mediterranea sexual|type=transcript|length=777bp
GTTTTCATAGTAACATACTCAGTGTATTTTCAAATAGATTGAATTAAAAA
TTGGTAAATTGATATTTATTAATGCCCCCGAAAAAGAAAAAGGGTAAAAA
AGGTGGAAAGAAAAAAGGTGGTAAGAAAAGTGGGAAAAAACGTGAAGATT
TAGATATCGAACAAAAATTTTCAAAAATTGAAAATGAAGTAGTAAATCTC
CAAAATCATTTGATTCAAAGACGAGCATACGCAAGAAAGGCGAATTCTGA
AAGATTTCTTGCAGAAGACTTGCAACACAGAACGCAGCAACAACTGGAAA
GTTTTGAAAGTGATTCTAAAGAAGTAAATGCTTTTATGACAAAGCAATAT
AAAATTATGAAAATAACACTTGGCGCTGAGATCGACGAGTTGACATTAGA
ATTAAATAAAACCAAAATCGAATTAGAGTCCACTTCTATAAATCTTTCTC
AGACAATAAAAGAGAAAGAGAAAATAACAAGAGAAAAGGACAATGAAATA
AACAACTTAAAAACAGAAATAGAAAGCATCCATAAACAATATGATGCAAT
CATTAACGGGGTGCTGGATAAATTGCAGAACCATTTATTAGTTGACAAAG
ATCAATGGCAAACGAGAACGGTTGCGTTGCACAGCAATAGTAAAAAGGCG
CTTTTAGATTTTGGTATCACACATCAATTTATTTAGTGAATTTTATGTTA
AATTTTCTGTTGATTATATTTTCAAATTATTTTATACTAGAGTTTAAGAA
AATTTTTTAAAGTTTTCACACATTGAA
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protein sequence of SMED30014233-orf-1

>SMED30014233-orf-1 ID=SMED30014233-orf-1|Name=SMED30014233-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=205bp
MPPKKKKGKKGGKKKGGKKSGKKREDLDIEQKFSKIENEVVNLQNHLIQR
RAYARKANSERFLAEDLQHRTQQQLESFESDSKEVNAFMTKQYKIMKITL
GAEIDELTLELNKTKIELESTSINLSQTIKEKEKITREKDNEINNLKTEI
ESIHKQYDAIINGVLDKLQNHLLVDKDQWQTRTVALHSNSKKALLDFGIT
HQFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR033585CCDC153
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
Vocabulary: biological process
TermDefinition
GO:0008150biological_process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 104..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
IPR033585Coiled-coil domain-containing protein 153PANTHERPTHR28656FAMILY NOT NAMEDcoord: 1..201