cilia- and flagella-associated protein 99
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014219 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10
Homology
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Human
Match: CFAP99 (cilia and flagella associated protein 99 [Source:HGNC Symbol;Acc:HGNC:51180]) HSP 1 Score: 219.935 bits (559), Expect = 9.750e-62 Identity = 173/563 (30.73%), Postives = 307/563 (54.53%), Query Frame = 1 Query: 76 MSLYEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLN-ISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNK--PTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQ----------DKKDPYFLMKEADRLQPACAQ----REISQKTLEKLN-QIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERA-KALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 M+ Y K + +E L+ F + +E +L +S + + F+++V + ++Y+K+L +V++ FY DGR + + + V+ +L F LEE+GF+ F + +++Q + M KFL F + +L +WI+ EW +Y+ V+ ++ L + P+++ + ++ + ++ P KT+ + T+P F+L P+P+ IP PE +P + K + +P +TY PK+ +++ L+ A+ + CA RE Q + ++L Q K K Y+ P N +K NT ILRE LY+ ++ ++ Q + KL +GA + +F WQ+ +++ D E+++ E +L GK+S+EEA+L R L+QENK+ V+ K++ AK + + E+ L E+R+ K LVEQVI Q+ K A+ + K +Q +E EESR L + A+ E RR+ ++I+++RALE + K++D T+I GYGL EMS+ EL+ERL L K+ +KR+ I K K +++ E + +CR+ + Sbjct: 1 MAYYGKCIETVIEQLDKFTPKRDNPEQFLEAAATSLQALSPQKQSFVLEVLSGCLEYRKLLTVVVDAFYVEDGRLCLRVDHSRFEVICYLATFLLEELGFQLFCNIIKSQPVDKMCKFLRFFFNPLHLCSWIKDEWSLIYEPAHVKENWIDPLMRWQPEVQELINHLEGVSASQSSPLKTKAKV--TEPKEFNLTAPRPRTIPAPEPVPVVAKPRPVPQSTYQ----PPKEQQQLETVKRYNRRKAEELLLRANIEELRCAMPRSCRERVQGSKQQLRLQFPPRIRKTPKLTFYR----PDNIL-----VKLNTTAILREGALYQRQVEQELQRVDKLVDGAGDFSEFFEWQKKMQAKDREEQLAASECRRLQGKLSHEEAVLARQSLMQENKQRVEQQKEQMAKLMLQR-AERRLREDRSRKELVEQVIEGQKNAKAAQTKLAKGRQQTVQEAIEESRGLLQRRAQAAQEEQRRRCELISQLRALETQPTRKGKLVDLTQIPGYGLEGEMSIVELRERLALLKENQRRKEEEKRDQIIQGKHTKSQELQNMVEQISLCRAAM 547
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Human
Match: CFAP99 (cilia and flagella associated protein 99 [Source:HGNC Symbol;Acc:HGNC:51180]) HSP 1 Score: 219.164 bits (557), Expect = 5.588e-61 Identity = 173/563 (30.73%), Postives = 307/563 (54.53%), Query Frame = 1 Query: 76 MSLYEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLN-ISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNK--PTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQ----------DKKDPYFLMKEADRLQPACAQ----REISQKTLEKLN-QIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERA-KALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 M+ Y K + +E L+ F + +E +L +S + + F+++V + ++Y+K+L +V++ FY DGR + + + V+ +L F LEE+GF+ F + +++Q + M KFL F + +L +WI+ EW +Y+ V+ ++ L + P+++ + ++ + ++ P KT+ + T+P F+L P+P+ IP PE +P + K + +P +TY PK+ +++ L+ A+ + CA RE Q + ++L Q K K Y+ P N +K NT ILRE LY+ ++ ++ Q + KL +GA + +F WQ+ +++ D E+++ E +L GK+S+EEA+L R L+QENK+ V+ K++ AK + + E+ L E+R+ K LVEQVI Q+ K A+ + K +Q +E EESR L + A+ E RR+ ++I+++RALE + K++D T+I GYGL EMS+ EL+ERL L K+ +KR+ I K K +++ E + +CR+ + Sbjct: 1 MAYYGKCIETVIEQLDKFTPKRDNPEQFLEAAATSLQALSPQKQSFVLEVLSGCLEYRKLLTVVVDAFYVEDGRLCLRVDHSRFEVICYLATFLLEELGFQLFCNIIKSQPVDKMCKFLRFFFNPLHLCSWIKDEWSLIYEPAHVKENWIDPLMRWQPEVQELINHLEGVSASQSSPLKTKAKV--TEPKEFNLTAPRPRTIPAPEPVPVVAKPRPVPQSTYQ----PPKEQQQLETVKRYNRRKAEELLLRANIEELRCAMPRSCRERVQGSKQQLRLQFPPRIRKTPKLTFYR----PDNIL-----VKLNTTAILREGALYQRQVEQELQRVDKLVDGAGDFSEFFEWQKKMQAKDREEQLAASECRRLQGKLSHEEAVLARQSLMQENKQRVEQQKEQMAKLMLQR-AERRLREDRSRKELVEQVIEGQKNAKAAQTKLAKGRQQTVQEAIEESRGLLQRRAQAAQEEQRRRCELISQLRALETQPTRKGKLVDLTQIPGYGLEGEMSIVELRERLALLKENQRRKEEEKRDQIIQGKHTKSQELQNMVEQISLCRAAM 547
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Zebrafish
Match: cfap99 (cilia and flagella associated protein 99 [Source:ZFIN;Acc:ZDB-GENE-161107-1]) HSP 1 Score: 284.648 bits (727), Expect = 5.904e-86 Identity = 184/557 (33.03%), Postives = 326/557 (58.53%), Query Frame = 1 Query: 85 YEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLNI-SEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQEKETYKA 1731 Y++ +N L+ F +D + + ED KNL S D+ FI++ I ++K+L +V+N FY G+NIF ++N++ V+ +L +F L+++G E F +++ M+KFL+F + NNL T I+ EW +YD+ V+ + L ++ ++++ + R NT+P FDL KPK + +P PE+IP+ +K K +P++T+ I+ D + + ++ + + EA+ Q CA + S+KT +++I+ +RE +LKF++ + +P S ++ NT ILRE LY ++ + Q L++L +GA F WQ+ ++ DL E++++LE+ +L G+ISYEEA+L R+R+++ N+ Q K+E A+ ++K+ +++ E+ + LV+QV + K AK +Q+ KQ I +E++E+SR L + L EA+AE+ RK+++I +IRA E QK +D TE AG+ LLCEMSLAEL+ERL L ++ + ++ +R+ I KQ K++ + E + + + R NL E+ ++ Sbjct: 3 YKELVNEVARFLDEFQEDKQCIDSFTEDTAKNLQTRSSADQKFIINTLYGCIMHKKLLNVVVNIFYLHHGKNIFRVDRNLFTVVCYLAMFCLDDLGLEHFSRIIKSLDNAKMHKFLSFFFNVNNLTTHIQKEWSHIYDAAIVDENWITPLLSWCSEMEMLLDQLTIKMGMGSVPKRSPRKNTEPKEFDLTKPKARPLPAPEVIPQQDKPKPVPASTHRSPKEPEILDDLR-QMNRLKAFQTLNEANSQQFRCANPQKSEKTQNIISEIIKKREAELKFDKTYTSGSPATQKTNSLPVRLNTTAILREGALYNRQLEGELQRLEQLSQGASEPSSFLQWQKEMKEKDLQEELAELERRRLEGRISYEEAVLARERVLKHNRYKAQETKEETAELMRKYAEKRLKEEKEMRELVQQVADGHKSSKAAKARLQEIKQRIVKEVSEQSRELLSQALEEAQAELSRKMELIRQIRAFELVPMVRQKFIDETETAGHDLLCEMSLAELRERLALLRESEQCDLEDRRQRIFQEKQFKEQLLLEQLDNIAL-RRNLAEQAALRS 557
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Xenopus
Match: CFAP99 (cilia and flagella associated protein 99 [Source:NCBI gene;Acc:100495334]) HSP 1 Score: 209.92 bits (533), Expect = 3.019e-59 Identity = 172/514 (33.46%), Postives = 291/514 (56.61%), Query Frame = 1 Query: 172 FKNLNISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISS-QLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEER-AKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 F++LN ++++ F+ S++Y+ +L V+ FY DG++ SE+ ++ V+ +L F+LEE+G + F + +Q + M KFL+F + NL TWI+ EW ++YDS +V+ ++ L K P++ E +++ P++ R + T+ F+L KP+P+ +P+P+LIP + + +P T Y PK+ + L+KE QK K N K++ A + Q + +K N A ILRE LY+ ++ ++ + ++KL +GA + +F WQ+ +R DL ++++++E +L GK+S EEA+L R LIQENK+ + KK+ +++ + + EK + EE+ + LVEQV + K A+L +QK KQ I +E+ EESR L + L EAE E+++K ++I EIRA+E K +D T+ AG+ L CEMSL EL+ERL + K+ ++ +KR I KQ KQ+ + +T E + + RS L Sbjct: 36 FQSLN--DEEERFVGAALCGSVQYRALLDTVVRAFYARDGKHCKQSEQYLFVVIGYLATFQLEELGLQTFVRIITSQDVTKMCKFLSFFFNVVNLNTWIKDEWSQIYDSNYVKENWISHLLKWQPEIHNITEGLQSKTAKGPSRARSS-KTTETKEFNLTKPRPRSLPVPQLIPAQKPHQPVPQTLYKA----PKEQE-----LLKE--------------QK----------------KRNRRKAESACCFSFQADNVPVKLNAAAILREGALYQRQVEEELRRIEKLVDGARDPTEFLEWQRQMREKDLEQQLAEIECRRLEGKLSREEAVLARQNLIQENKKKADI-KKQETEKLMRIYAEKRIQEEKEMRDLVEQVSEGHKNTKQARLKLQKYKQQIVQEVTEESRELLRQALEEAEEELKKKFELIREIRAMESVPFIRHKFVDLTQTAGHRLNCEMSLVELRERLAILKEAEKKAEEEKRNQILIEKQSKQQLLLDTLEQISLHRSAL 506
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Mouse
Match: Cfap99 (cilia and flagella associated protein 99 [Source:MGI Symbol;Acc:MGI:5434801]) HSP 1 Score: 249.21 bits (635), Expect = 1.246e-72 Identity = 191/567 (33.69%), Postives = 326/567 (57.50%), Query Frame = 1 Query: 76 MSLYEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNL--NISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNI-LDNKPT-KTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDK----------KDPYFLMKEADRLQPACA----QREISQKTLEK--LNQIV--IEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERA-KALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 M+ YEK + ++ L+ F + VE F +L +S + F+++V + ++Y K+L IV++ FY DG S+ +++ V+ +L IF+LEE+GF+ F S +++Q ++ M KFL F + NL +WI+ EW +Y++ V ++ L + P++++ +++++ + D P KT+ + T P F+L P+P+ I MPE +P ++K + +P +TY +PK+ K + L+ +A+ + CA QRE ++ +EK +Q V I R +L F YK P +K NTATILRE LY+ ++ K+ Q + KL +GA + +F WQ+ +++ DL E+++ E +L GK+S+EEAIL R L+QENK+ K+E A+ + + E+ L EE+A K LVEQV +Q+ VK+ + + K +Q I +E+ EESR L + EA+ E +R+ ++IA +RA+E + K++D T+I GYGL EMS+ EL+ERL + K+ + +KR+ I +K+ K + I T E + +CR+ + Sbjct: 1 MTYYEKCIEIVIKQLDKFKPGKDNPEQFVETVFTSLQQTLSPQKFGFVLEVLSGCLEYHKLLTIVVDAFYARDGHTCLWSDYSLFEVICYLAIFQLEELGFQLFCSIIKSQPVRKMCKFLRFFFNPLNLSSWIKDEWSLIYETAHVMENWIDPLLRWQPEVQKLIKQLEGVPTDQIPVLKTKAKV--TVPKEFNLTVPRPRAITMPEPVPTMDKPRPVPQSTYK----EPKEQKLLQIIKTCNRRTAEELLLKANMEELQCAMPRVQREPQEQDVEKQLWHQSVPHIRRTPNLTF--YKPNNLP---------VKLNTATILREGALYQRQVEKELQRVDKLVDGAGDFSEFLKWQKKMQAQDLEEQLAAGECRRLQGKLSHEEAILARQNLMQENKQKADRQKEETAELMLQ-CAERRLQEEKAMKELVEQVTEAQKNVKIVQTKLVKGRQQIVQEVIEESRELLQRSAKEAKEEQKRRCELIAHLRAMETQPTRKGKLVDLTQIPGYGLEGEMSVVELRERLAMLKETQKREQEEKRDQIIQDKRAKSQMIQNTVEQISLCRAAM 549
BLAST of cilia- and flagella-associated protein 99 vs. UniProt/SwissProt
Match: sp|D6REC4|CFA99_HUMAN (Cilia- and flagella-associated protein 99 OS=Homo sapiens OX=9606 GN=CFAP99 PE=3 SV=1) HSP 1 Score: 148.673 bits (374), Expect = 2.969e-37 Identity = 95/254 (37.40%), Postives = 155/254 (61.02%), Query Frame = 1 Query: 949 LKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERA-KALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 +K NT ILRE LY+ ++ ++ Q + KL +GA + +F WQ+ +++ D E+++ E +L GK+S+EEA+L R L+QENK+ V+ K++ AK + + E+ L E+R+ K LVEQVI Q+ K A+ + K +Q +E EESR L + A+ E RR+ ++I+++RALE + K++D T+I GYGL EMS+ EL+ERL L K+ +KR+ I K K +++ E + +CR+ + Sbjct: 40 VKLNTTAILREGALYQRQVEQELQRVDKLVDGAGDFSEFFEWQKKMQAKDREEQLAASECRRLQGKLSHEEAVLARQSLMQENKQRVEQQKEQMAKLMLQRA-ERRLREDRSRKELVEQVIEGQKNAKAAQTKLAKGRQQTVQEAIEESRGLLQRRAQAAQEEQRRRCELISQLRALETQPTRKGKLVDLTQIPGYGLEGEMSIVELRERLALLKENQRRKEEEKRDQIIQGKHTKSQELQNMVEQISLCRAAM 292
BLAST of cilia- and flagella-associated protein 99 vs. UniProt/SwissProt
Match: sp|A8IUG5|CFA99_CHLRE (Cilia- and flagella-associated protein 99 OS=Chlamydomonas reinhardtii OX=3055 GN=CFAP99 PE=1 SV=1) HSP 1 Score: 102.449 bits (254), Expect = 4.364e-21 Identity = 154/566 (27.21%), Postives = 264/566 (46.64%), Query Frame = 1 Query: 88 EKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLNISEK-DKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRK--------------AIPNTKPAPFDLHKPKPKRIPM---------------PELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKT-LEKLNQ-IVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKAL-VEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEE----MLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIA-GYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAE 1671 EK + C +I +F +L HV++ L++ D FI V +++Y+++L +++ FY +G + ++Y + +L IFRLEE+ F F+ ++ Q MY L ++ + + + +W KLYD FV +E+++ L + +EM L+ K T +RK A T P PF+L +PKP+ +P+ P P E+V + + + K K P+ L R + + T ++K+ + + ER ++L F ++ ++Q++ N A ILRE LY + ++ LK+ E + F AWQ ++ D + + + +E+ + + E AI R + N EL + K+E AKRI++ K + R AL + V+ +++ V+ A V+K Q R AEE RR Q+E A EM + D+I +++ALE K DPTE +GLL MSL EL+ERL ++K++ E ++R I KQE++ + E Sbjct: 43 EKLMAACEKIYKSFNPAQVTLDTHVDNCIGQLSVHNSFDDSFIRQVVYGTVRYRRLLGALMDSFYHYNGGAASREDVDMYKLYSYLTIFRLEELNFSNFQRLVDAMTPQKMYVLLKYMFNPQYMREVVREDWLKLYDKEFV-DEIID----RLLSWKSESKEMLGRLEYKVTLSRKEDDEKETLGTGYAAARSTTVPQPFNLTQPKPRPLPVEDPPPPPIRAKPAPRPREGPTKEEVALAAAREANRAAAERKAAKAAPFKL-------------RVLERPTNIDKIREELEAERTRELTFKGIRAA---PPPPVPNAQVRLNAAAILREDALYRRKQQEEADALKRYEAELRDASSFKAWQNAMLEQDEAARAASVERRRQEMAAAQENAIRARMAAQEANAELARAAKEE-AKRIEEDLKRDREEQARLNALRRDAVVEARQNVQAA---VEKMSQE-RRLAAEEERRKQQEDARARAEVAAREMAERRDIILQLKALEKVPKQRVKEFDPTETGPDHGLLETMSLVELRERLNVAKRRQREEEERQRAEILRQKQERESALLE 582
BLAST of cilia- and flagella-associated protein 99 vs. TrEMBL
Match: A0A2R2MSK6 (cilia- and flagella-associated protein 99 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106177570 PE=4 SV=1) HSP 1 Score: 337.806 bits (865), Expect = 8.432e-104 Identity = 215/553 (38.88%), Postives = 347/553 (62.75%), Query Frame = 1 Query: 76 MSLYEKKL-NHCVEILNTFPKDCKSLTIHVEDYFKNL-NISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 MSL K+L HCVE+L+TF + +S+ HV +Y L NI + D+ FI +VF+ ++Y +L +V++ FY G++ SE N+Y VL +L +FRL+E+ ++ ++++Q + MYKFLNF D+ NL TWI+ EW KLY+ FV+ L++ L + LP+L+ ++++ + + NK ++ IP T+P F+L +P+P+ +PMPE IP++EK K +P TTY I+ + K +K LM E+ R Q ACA E S KT +L I+ E+E L+F+++K+ P P S+ + +K N ATILRE KL++ R ++ ++L++LE GA + F WQQ +R D+ +++++E+ +L GK+S+E+AIL R LI++NK+ V +K+E ++++ ++K E+ + LVEQ + + K AK ++ K+ I +++ EES+ L + L EAEAEMRRK+++I +IRA+E + K +D T+ AGYGLL E SLAELKERL L K E +KR+ I +K+ K + + T E + RS + Sbjct: 1 MSLAHKQLLEHCVEVLDTFQPELRSVEQHVNNYMAKLKNIEDSDQTFITEVFSGCVRYDSVLNVVLDGFYIRSGKSSLRSESNLYKVLTYLALFRLDELEVAHYRKFVKSQDVNKMYKFLNFFMDDKNLRTWIKDEWNKLYERPFVQMTLLSPLLRWLPELQEMIQQLADKIANKVKPKKQIIPTTEPKAFNLTQPRPRSVPMPEPIPKLEKHKPVPKTTYQDPKQKRIIEENKVMNRRKAEERLM-ESSRYQFACANPEKSDKTRHRLQSILAEQESKLEFDKHKANPLPNFVSE-NIPIKLNAATILREGKLFQKREEEELRKLERLEAGAKDASDFLRWQQEMRKQDMDRQLAEIEQRRLEGKLSHEDAILARQNLIKDNKQKVTEMKEEAKAIMQEYLEKKFKEEQEMRVLVEQTMEGHQNTKEAKKKLKDYKRKIVQDVNEESKELMRQALEEAEAEMRRKMEIIQQIRAMEAVPAIRVKFVDFTKTAGYGLLSEFSLAELKERLALMKMAEREAEEEKRDEILASKEAKDQLLMNTLETISKHRSEM 551
BLAST of cilia- and flagella-associated protein 99 vs. TrEMBL
Match: A0A1S3K0F9 (cilia- and flagella-associated protein 99 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106177570 PE=4 SV=1) HSP 1 Score: 338.576 bits (867), Expect = 7.414e-103 Identity = 215/553 (38.88%), Postives = 347/553 (62.75%), Query Frame = 1 Query: 76 MSLYEKKL-NHCVEILNTFPKDCKSLTIHVEDYFKNL-NISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 MSL K+L HCVE+L+TF + +S+ HV +Y L NI + D+ FI +VF+ ++Y +L +V++ FY G++ SE N+Y VL +L +FRL+E+ ++ ++++Q + MYKFLNF D+ NL TWI+ EW KLY+ FV+ L++ L + LP+L+ ++++ + + NK ++ IP T+P F+L +P+P+ +PMPE IP++EK K +P TTY I+ + K +K LM E+ R Q ACA E S KT +L I+ E+E L+F+++K+ P P S+ + +K N ATILRE KL++ R ++ ++L++LE GA + F WQQ +R D+ +++++E+ +L GK+S+E+AIL R LI++NK+ V +K+E ++++ ++K E+ + LVEQ + + K AK ++ K+ I +++ EES+ L + L EAEAEMRRK+++I +IRA+E + K +D T+ AGYGLL E SLAELKERL L K E +KR+ I +K+ K + + T E + RS + Sbjct: 1 MSLAHKQLLEHCVEVLDTFQPELRSVEQHVNNYMAKLKNIEDSDQTFITEVFSGCVRYDSVLNVVLDGFYIRSGKSSLRSESNLYKVLTYLALFRLDELEVAHYRKFVKSQDVNKMYKFLNFFMDDKNLRTWIKDEWNKLYERPFVQMTLLSPLLRWLPELQEMIQQLADKIANKVKPKKQIIPTTEPKAFNLTQPRPRSVPMPEPIPKLEKHKPVPKTTYQDPKQKRIIEENKVMNRRKAEERLM-ESSRYQFACANPEKSDKTRHRLQSILAEQESKLEFDKHKANPLPNFVSE-NIPIKLNAATILREGKLFQKREEEELRKLERLEAGAKDASDFLRWQQEMRKQDMDRQLAEIEQRRLEGKLSHEDAILARQNLIKDNKQKVTEMKEEAKAIMQEYLEKKFKEEQEMRVLVEQTMEGHQNTKEAKKKLKDYKRKIVQDVNEESKELMRQALEEAEAEMRRKMEIIQQIRAMEAVPAIRVKFVDFTKTAGYGLLSEFSLAELKERLALMKMAEREAEEEKRDEILASKEAKDQLLMNTLETISKHRSEM 551
BLAST of cilia- and flagella-associated protein 99 vs. TrEMBL
Match: A0A2R2MS83 (cilia- and flagella-associated protein 99 isoform X3 OS=Lingula unguis OX=7574 GN=LOC106177570 PE=4 SV=1) HSP 1 Score: 337.035 bits (863), Expect = 2.425e-102 Identity = 214/553 (38.70%), Postives = 347/553 (62.75%), Query Frame = 1 Query: 76 MSLYEKKL-NHCVEILNTFPKDCKSLTIHVEDYFKNL-NISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNL 1707 MSL K+L HCVE+L+TF + +S+ HV +Y L NI + D+ FI +VF+ ++Y +L +V++ FY G++ SE N+Y VL +L +FRL+E+ ++ ++++Q + MYKFLNF D+ NL TWI+ EW KLY+ FV+ L++ L + LP+L+ ++++ + + NK ++ IP T+P F+L +P+P+ +PMPE IP++EK K +P TTY I+ + K +K LM E+ R Q ACA E S KT +L I+ E+E L+F+++K+ P P S+ + +K N A+ILRE KL++ R ++ ++L++LE GA + F WQQ +R D+ +++++E+ +L GK+S+E+AIL R LI++NK+ V +K+E ++++ ++K E+ + LVEQ + + K AK ++ K+ I +++ EES+ L + L EAEAEMRRK+++I +IRA+E + K +D T+ AGYGLL E SLAELKERL L K E +KR+ I +K+ K + + T E + RS + Sbjct: 1 MSLAHKQLLEHCVEVLDTFQPELRSVEQHVNNYMAKLKNIEDSDQTFITEVFSGCVRYDSVLNVVLDGFYIRSGKSSLRSESNLYKVLTYLALFRLDELEVAHYRKFVKSQDVNKMYKFLNFFMDDKNLRTWIKDEWNKLYERPFVQMTLLSPLLRWLPELQEMIQQLADKIANKVKPKKQIIPTTEPKAFNLTQPRPRSVPMPEPIPKLEKHKPVPKTTYQDPKQKRIIEENKVMNRRKAEERLM-ESSRYQFACANPEKSDKTRHRLQSILAEQESKLEFDKHKANPLPNFVSE-NIPIKLNAASILREGKLFQKREEEELRKLERLEAGAKDASDFLRWQQEMRKQDMDRQLAEIEQRRLEGKLSHEDAILARQNLIKDNKQKVTEMKEEAKAIMQEYLEKKFKEEQEMRVLVEQTMEGHQNTKEAKKKLKDYKRKIVQDVNEESKELMRQALEEAEAEMRRKMEIIQQIRAMEAVPAIRVKFVDFTKTAGYGLLSEFSLAELKERLALMKMAEREAEEEKRDEILASKEAKDQLLMNTLETISKHRSEM 551
BLAST of cilia- and flagella-associated protein 99 vs. TrEMBL
Match: A0A210PYE8 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT17394 PE=4 SV=1) HSP 1 Score: 323.553 bits (828), Expect = 3.169e-97 Identity = 201/553 (36.35%), Postives = 329/553 (59.49%), Query Frame = 1 Query: 73 MMSL-YEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLNI-SEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQL--KTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICR 1698 M SL + + L H V +L+T+ +D +S+ HV K I E D+ FIV+VF+ ++Y ++ ++ + F+ DG+N+ SE+N+Y V +L FRL+E+G E F+ ++ Q + MY+FLNF +E NL TW++ W K Y+ FV+ +++ +++ +P+L+ + MK +DNK RK P T+ PFD+ +P+P+ IP+PE IP++ K K P + Y ++ + +K LM EA R+Q ACA E S KT E N I+ E + L F +++KP P S +S ++ K NT ILRE LY R K+ + + LE GA + +F+ WQ +++ DL ++++ +E+ +L GK+S+EEAIL R L++ NK+ V ++E K +F +K E+ + LVEQ + + K A +Q+ K+ I +++ EESR L ++ + EAE+EMRR++++I +IRA+E QK +D T +G+GLL EMS+AEL+ER+ L EE Q+R+ I +KQ K +++ +T E + R Sbjct: 1 MASLSHGQLLVHAVRVLDTYNRDMQSVEEHVNKCLKAQRIYDEGDQTFIVEVFSNCVRYWDLMKVITDGFFVKDGKNVLRSEENLYIVTSYLAFFRLDELGVEHFRKFVSAQDINKMYRFLNFCLNERNLLTWMKDGWEKFYEHTFVQTGILSPIQRWMPELQEMLTHMKAKIDNKIKPRRKTTPKTETRPFDITQPRPRSIPIPEAIPKLHKHKPPPRSLYDDPEEFDVLKRNRDVNRRKAEERLM-EASRIQFACANPEKSDKTKEIWNNIISEEDGKLDFERHRAKPTP---SFLSKEVPFKMNTTAILREGHLYSQREEKEIKRFENLEAGAKDKSEFEKWQNKMKTQDLEQELAAIERRRLEGKLSHEEAILARQNLMRTNKQKVLNHREETKKMFDEFLHQKFKEEQEMRTLVEQTMQGHKNAKDATKKIQEIKRKIVQDVNEESRELMKQAVEEAESEMRRRMELIHQIRAMEAVPIIRQKFVDLTATSGHGLLSEMSIAELRERMSLLATAEEELEEQRRDDILASKQAKDQKLLDTLETISRHR 549
BLAST of cilia- and flagella-associated protein 99 vs. TrEMBL
Match: A0A267FQQ8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig017297g1 PE=4 SV=1) HSP 1 Score: 322.398 bits (825), Expect = 1.350e-96 Identity = 195/538 (36.25%), Postives = 321/538 (59.67%), Query Frame = 1 Query: 97 LNHCVEILNTFPKDCKSLTIHVEDYFKN-LNISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYI----VSDFKPKQDKKDPYF--LMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQIS-SQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIV 1686 L H EIL++F ++ H YF L + +V IV++FA I+Y+K L VI FY +GR + SE+N+Y V +L++FR+EE+G K + T + + +Y+FL+F+ + +L+TW+ EW KLYD ++VENE+++ L++ LP L +E ++ ++ ++ I T+P PF L +PKP+ +PMPE +P + + +PS+TY +D ++ K + EA R +P CA S+K K+ +I E+E+ L F+ KS P PK + +K N A I+REW Y ++ + L LE GAF+ F+AWQQ+ + ADL ++++LE+ +L G++SYEEA++ R RL + N+ +V+ ++ A+R ++ +A EE+ K + +++A + A QK K E +E+ L ++ EA AE+RRK+++IAEIRA+E +Q+ +K LD TEIAG+G L EMS+AEL+ERL L+KQ+ + V +KR+ I + KQ K +++ T E++ Sbjct: 14 LQHSQEILDSFRSADMAVDAHANQYFDGVLPRDDPVRVTIVEIFAGCIRYEKALDTVITAFYNVEGRVVKRSEQNLYRVFAYLIVFRMEELGIPNLKKLIATLERKRIYRFLSFLLNRKHLYTWMLDEWAKLYDRVYVENEIISPLEEFLPQLEEILERLRVQIEEGLKSKKELIKPTEPQPFTLTEPKPRSVPMPEELPTLAPSRPVPSSTYERPQESADLAERRRKNREVAEQRLAEATRQRPRCADPGKSEKCERKIREIRAEQERALAFDAPKSNPMPKERQGAGQAAVKLNAAAIMREWARYREAEKREAERLGALEAGAFDKSAFEAWQQAQKEADLQSQLAELERRRLEGQLSYEEAVIARRRLAESNRAVVEKEREAAAERQEELAAMRAAEEEQLKQRMAEIMAGHAKARQAVRDAQKAKALSRAEQQQETEELLKQAYEEAAAELRRKMELIAEIRAIEATQAPREKRLDLTEIAGFGFLSEMSIAELRERLSLAKQRQTQEVEEKRDQILEEKQAKDEKLLHTMELI 551
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Cavefish
Match: cfap99 (cilia and flagella associated protein 99 [Source:NCBI gene;Acc:103035029]) HSP 1 Score: 273.863 bits (699), Expect = 6.028e-82 Identity = 186/555 (33.51%), Postives = 319/555 (57.48%), Query Frame = 1 Query: 109 VEILNTFPKDCKSLTIHVEDYFKNL-NISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPN-------TKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQEKETYK 1728 + +L+ + D +S+ +ED K L + S D+ F +DV + Y+++L +V+N Y G+++F +++ Y V+ +L +F LE++GFE+F +++ M+KFL+F + NL TWI EW + YD+ +VE+ K + L R+ E++ +LD+ K K+ + T+P FDL KPKP+ +P PE IP+ EK K +P+TT+ ++ + K K K L EA+ Q CA + S+KT ++QI + LKF+ + P + ++ NT ILRE LY ++ ++ L++L +G F WQ+ ++ DL E+++Q+E+ +L GKIS+EEA+L R R+++ N+ L K+E A+ ++++ +++ E+ K LV+QV + K AK+ + + KQ I RE++E+SR L ++ L EA+AE+ RK ++I +IRALE S K +D TE G+ LLCEMSLAEL+ERL +++ ++ +KR+ I + KQ K++ + E + + I R+ + + K Sbjct: 11 ISLLDKYNPDEQSVDSFIEDSVKTLGDHSSGDQKFFIDVVYGCVVYRRLLDVVVNVLYNQTGKSLFKADRTQYVVVCYLAMFHLEDLGFEQFSRIIKSLDSSKMHKFLSFFFNVTNLTTWIHGEWSQFYDAAYVEH-------KWIAPLLRWRTEIEGLLDHLAKKMTKSSLSRNSPKKYTQPKEFDLTKPKPRPLPAPECIPQQEKAKPVPATTHRTPKEPNVLEESKQKNHLKAQQVLY-EANIQQFRCANPQTSEKTKNTVSQIQQSYDAKLKFDNVYTSGTPATHKMNDLPIRMNTTAILREGALYNRQMEEELNRLERLMQGGNEPSVFLQWQREMKEKDLQEELAQVERRRLEGKISHEEALLARQRVLELNQHKAHLKKEETAELMRRYAEKRLTEEKEIKELVQQVANGHKNSKAAKMKLHELKQCIVREVSEQSRELLQQALEEAQAELSRKFELIRQIRALESVPSIRHKFVDDTETGGHDLLCEMSLAELRERLARLREEEQQEQEEKRQKILEEKQSKEQLLLEQLDTIAIRRAGAGQAQARK 557
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Cavefish
Match: cfap99 (cilia and flagella associated protein 99 [Source:NCBI gene;Acc:103035029]) HSP 1 Score: 257.684 bits (657), Expect = 2.497e-76 Identity = 183/555 (32.97%), Postives = 311/555 (56.04%), Query Frame = 1 Query: 109 VEILNTFPKDCKSLTIHVEDYFKNL-NISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPN-------TKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTY-------IVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQEKETYK 1728 + +L+ + D +S+ +ED K L + S D+ F +DV + Y+++L +V+N Y G+++F +++ Y V+ +L +F LE++GFE+F +++ M+KFL+F + NL TWI EW + YD+ +VE+ K + L R+ E++ +LD+ K K+ + T+P FDL KPKP+ +P PE IP+ EK K +P+TT+ ++ + K K K L EA+ Q CA + S+KT K+N + I + NT ILRE LY ++ ++ L++L +G F WQ+ ++ DL E+++Q+E+ +L GKIS+EEA+L R R+++ N+ L K+E A+ ++++ +++ E+ K LV+QV + K AK+ + + KQ I RE++E+SR L ++ L EA+AE+ RK ++I +IRALE S K +D TE G+ LLCEMSLAEL+ERL +++ ++ +KR+ I + KQ K++ + E + + I R+ + + K Sbjct: 11 ISLLDKYNPDEQSVDSFIEDSVKTLGDHSSGDQKFFIDVVYGCVVYRRLLDVVVNVLYNQTGKSLFKADRTQYVVVCYLAMFHLEDLGFEQFSRIIKSLDSSKMHKFLSFFFNVTNLTTWIHGEWSQFYDAAYVEH-------KWIAPLLRWRTEIEGLLDHLAKKMTKSSLSRNSPKKYTQPKEFDLTKPKPRPLPAPECIPQQEKAKPVPATTHRTPKEPNVLEESKQKNHLKAQQVLY-EANIQQFRCANPQTSEKT--KMNDLPI---------------------------RMNTTAILREGALYNRQMEEELNRLERLMQGGNEPSVFLQWQREMKEKDLQEELAQVERRRLEGKISHEEALLARQRVLELNQHKAHLKKEETAELMRRYAEKRLTEEKEIKELVQQVANGHKNSKAAKMKLHELKQCIVREVSEQSRELLQQALEEAQAELSRKFELIRQIRALESVPSIRHKFVDDTETGGHDLLCEMSLAELRERLARLREEEQQEQEEKRQKILEEKQSKEQLLLEQLDTIAIRRAGAGQAQARK 528
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Medaka
Match: cfap99 (cilia and flagella associated protein 99 [Source:NCBI gene;Acc:101156371]) HSP 1 Score: 154.451 bits (389), Expect = 2.600e-39 Identity = 155/557 (27.83%), Postives = 275/557 (49.37%), Query Frame = 1 Query: 85 YEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLNISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTK-------PAPFDLHKPKPKRIPMPELIPEIEKVKEIPST-------TYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQE 1713 Y + + +L+TF +SL +E+ ++L + + FI+D+F+ I+ +K L +V+N FY T+G+ + ++N + ++ +L + + +E+ ++NMY FLNF +NL + I+ EW + D+ FVE +N L R+ E+ N+L KT + P K P F KPKP+ + +PEL PE EK K +PS+ +I+ + K K+ +K F +++ + S + EKL + S S +K N A ILR L + ++ ++ Q + L +G F WQ+ + + D ++ EK +L +I E L R++++ N++ Q K+E A+ +++ +E+ E++ K LV+QV S + K AK ++K KQ I +E++EES+ L + E +AE+ ++ +MI E A + D T+ AG+GLL EM LAEL+ RL L K + +E ++R I KQ++++ + + E +++ L E Sbjct: 5 YGSLVKKAIGLLDTFGSKIQSLDAFMEEALQDL----QQRNFILDIFSGCIENKKQLDVVVNAFYATNGKLMSRRDRNQFVMICYLTFSLDDLGLQNF-SNMIESIGIKNMYTFLNFYF--SNLTSSIKHEWSSIIDAAFVEEHWIN-------PLLRWRPEIYNLLSQLAAKTSQDNPKKKVTTKVTVPQEFSFSKPKPRLLSVPELPPEHEKTKPVPSSIHRSPKEMHILKEIKEKKHQKAELFYEANLKQIRMENPLKSESSEVFEKLWSRL---------------------SAGSFSVKLNKAAILRREALCDQQLEQELQRQEWLMQGGLEPSAFLQWQREMLTRD---QLPMTEKKQLEAQIRDERIALAREQIMDRNQKTAQQTKEEIAQLMQEIAEERIQEEQKKKELVQQVAESHKNTKEAKEKLKKLKQTIVKEVSEESQELLFQAFEEKQAELCKRFEMIHENHAKQSLPRIRLNPFDDTKTAGHGLLEEMPLAELRLRLDLLKMERQEEEQERRMHILQEKQKQEELLQKRLEAIELHSRALAE 523
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Medaka
Match: cfap99 (cilia and flagella associated protein 99 [Source:NCBI gene;Acc:101156371]) HSP 1 Score: 149.828 bits (377), Expect = 6.905e-38 Identity = 140/480 (29.17%), Postives = 246/480 (51.25%), Query Frame = 1 Query: 172 FKNLNISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTK-------PAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSK 1590 +N+N+ +++ FI+D+F+ I+ +K L +V+N FY T+G+ + ++N + ++ +L + + +E+ ++NMY FLNF +NL + I+ EW + D+ FVE +N L R+ E+ N+L KT + P K P F KPKP+ + +PEL PE EK K +PS+ + + K+ + L +EI +K +K ++ E +LK ++ +++ S +K N A ILR L + ++ ++ Q + L +G F WQ+ + + D ++ EK +L +I E L R++++ N++ Q K+E A+ +++ +E+ E++ K LV+QV S + K AK ++K KQ I +E++EES+ L + E +AE+ ++ +MI E A + D T+ AG+GLL EM LAEL+ RL L K Sbjct: 8 LQNINVQQRN--FILDIFSGCIENKKQLDVVVNAFYATNGKLMSRRDRNQFVMICYLTFSLDDLGLQNF-SNMIESIGIKNMYTFLNFYF--SNLTSSIKHEWSSIIDAAFVEEHWIN-------PLLRWRPEIYNLLSQLAAKTSQDNPKKKVTTKVTVPQEFSFSKPKPRLLSVPELPPEHEKTKPVPSSIH--------RSPKEMHIL-------------KEIKEKKHQKAEELFYE--ANLKQIRMENPLKSESSEAGSFSVKLNKAAILRREALCDQQLEQELQRQEWLMQGGLEPSAFLQWQREMLTRD---QLPMTEKKQLEAQIRDERIALAREQIMDRNQKTAQQTKEEIAQLMQEIAEERIQEEQKKKELVQQVAESHKNTKEAKEKLKKLKQTIVKEVSEESQELLFQAFEEKQAELCKRFEMIHENHAKQSLPRIRLNPFDDTKTAGHGLLEEMPLAELRLRLDLLK 449
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Medaka
Match: cfap99 (cilia and flagella associated protein 99 [Source:NCBI gene;Acc:101156371]) HSP 1 Score: 149.828 bits (377), Expect = 7.286e-38 Identity = 140/480 (29.17%), Postives = 246/480 (51.25%), Query Frame = 1 Query: 172 FKNLNISEKDKVFIVDVFAESIKYQKILMIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTK-------PAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSK 1590 +N+N+ +++ FI+D+F+ I+ +K L +V+N FY T+G+ + ++N + ++ +L + + +E+ ++NMY FLNF +NL + I+ EW + D+ FVE +N L R+ E+ N+L KT + P K P F KPKP+ + +PEL PE EK K +PS+ + + K+ + L +EI +K +K ++ E +LK ++ +++ S +K N A ILR L + ++ ++ Q + L +G F WQ+ + + D ++ EK +L +I E L R++++ N++ Q K+E A+ +++ +E+ E++ K LV+QV S + K AK ++K KQ I +E++EES+ L + E +AE+ ++ +MI E A + D T+ AG+GLL EM LAEL+ RL L K Sbjct: 8 LQNINVQQRN--FILDIFSGCIENKKQLDVVVNAFYATNGKLMSRRDRNQFVMICYLTFSLDDLGLQNF-SNMIESIGIKNMYTFLNFYF--SNLTSSIKHEWSSIIDAAFVEEHWIN-------PLLRWRPEIYNLLSQLAAKTSQDNPKKKVTTKVTVPQEFSFSKPKPRLLSVPELPPEHEKTKPVPSSIH--------RSPKEMHIL-------------KEIKEKKHQKAEELFYE--ANLKQIRMENPLKSESSEAGSFSVKLNKAAILRREALCDQQLEQELQRQEWLMQGGLEPSAFLQWQREMLTRD---QLPMTEKKQLEAQIRDERIALAREQIMDRNQKTAQQTKEEIAQLMQEIAEERIQEEQKKKELVQQVAESHKNTKEAKEKLKKLKQTIVKEVSEESQELLFQAFEEKQAELCKRFEMIHENHAKQSLPRIRLNPFDDTKTAGHGLLEEMPLAELRLRLDLLK 449
BLAST of cilia- and flagella-associated protein 99 vs. Planmine SMEST
Match: SMESG000025927.1 (SMESG000025927.1) HSP 1 Score: 1026.54 bits (2653), Expect = 0.000e+0 Identity = 570/572 (99.65%), Postives = 570/572 (99.65%), Query Frame = 1 Query: 256 MIVINRFYETDGRNIFLSEKNVYXXXXXXXXXXXXXXXXXXXKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQEKETYKAPIQPISLENLDENKLKEIKTLQAQLEEXXXXXXXXXXXXXXSNRIAXXXXXXXXXXXXLEKYPIKSQSDTVERMKQILSV 1971 MIVINRFYE DGRNIFLSEKNVYFVLLFLLIFRLEEIGFEEFKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQEKETYKAPIQPISLENLDENKLKEIKTLQAQLEEKRSQRKQQRQDQIKSNRIASSDKSV KSSGVLEKYPIKSQSDTVERMKQILSV Sbjct: 1 MIVINRFYEKDGRNIFLSEKNVYFVLLFLLIFRLEEIGFEEFKSYLETQKLQNMYKFLNFVTDENNLFTWIELEWRKLYDSIFVENELMNGLKKSLPDLRRYVEEMKNILDNKPTKTRKAIPNTKPAPFDLHKPKPKRIPMPELIPEIEKVKEIPSTTYIVSDFKPKQDKKDPYFLMKEADRLQPACAQREISQKTLEKLNQIVIEREKDLKFNEYKSKPAPKNNSQISSQLKTNTATILREWKLYETRITKKNQELKKLEEGAFNTQQFDAWQQSLRSADLSEKISQLEKLKLAGKISYEEAILKRDRLIQENKELVQLLKKENAKRIKKFFKEKALNEERAKALVEQVIASQEGVKVAKLTVQKKKQAIAREIAEESRRLQEEMLIEAEAEMRRKIDMIAEIRALEDSQSSNQKILDPTEIAGYGLLCEMSLAELKERLILSKQKYEENVNQKREMISDNKQEKQKQIAETFEIVKICRSNLQEKETYKAPIQPISLENLDENKLKEIKTLQAQLEEKRSQRKQQRQDQIKSNRIASSDKSVVKSSGVLEKYPIKSQSDTVERMKQILSV 572 The following BLAST results are available for this feature:
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of cilia- and flagella-associated protein 99 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of cilia- and flagella-associated protein 99 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of cilia- and flagella-associated protein 99 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of cilia- and flagella-associated protein 99 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014219 ID=SMED30014219|Name=cilia- and flagella-associated protein 99|organism=Schmidtea mediterranea sexual|type=transcript|length=2115bpback to top protein sequence of SMED30014219-orf-1 >SMED30014219-orf-1 ID=SMED30014219-orf-1|Name=SMED30014219-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=634bp MMSLYEKKLNHCVEILNTFPKDCKSLTIHVEDYFKNLNISEKDKVFIVDVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|