SMED30014212

Overview
NameSMED30014212
Smed IDSMED30014212
Length (bp)1293
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014212 (SMED30014212) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014212 (SMED30014212) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014212 (SMED30014212) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014212 (SMED30014212) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014212

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014212h1SMcG0009926 dd_Smed_v4_10207_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014212 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001257:35811..52080 -6343
Homology
[Close]
BLAST of SMED30014212 vs. TrEMBL
Match: A0A4Z2DIS9 (Uncharacterized protein OS=Schistosoma japonicum OX=6182 GN=EWB00_000503 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.848e-10
Identity = 71/306 (23.20%), Postives = 125/306 (40.85%), Query Frame = 1 
Query:  124 ILPKQNVLQGEKVNISCQFNSYISHLPWSL----DTKLLAENTKSYD------ILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKL--PFINPKK----------LSDTNATCSS----------------IETGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRVNCP------ENTKLYKITHTVNINCQHHKLVGRNEIRCQSQD-YHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKN 906
            I P Q VL G+KV + CQ       + +++      KL++E    Y+      +   + +Q    Y+L I++V+ +  G W C   A     TL +  P  L+   V  L  P  N +           LS T++  ++                I  G R  PYDL     +   CE+ CG P   +I+    N T T  +      ++N            + K T T+ I+C    ++G N+++C      + +++  +  Y+LCP      +LT GE + I +   L ++ + +   ++R N
Sbjct:   30 IPPNQPVLIGQKVILKCQVQGIRFPMQYAVLFKRKDKLISEKCHVYEGQKYHLVCEPNDWQRAETYELHIKSVSWADRGDWLCIYAANMTRQTLEVYAPAKLEKMGVSSLTLPMTNKESILKESGSFPGLSSTSSLAAAGMDSALSIIGTPPSQHIGLGTRTNPYDLRPGLGIQFTCETTCGHPR-ANITWLVINETMTKAIMPTISNKINYDYCNNSLNTINMTKTTSTLQIHCTQLDIIGLNQVQCSVNGPQYKDILLTRNVYVLCPGGERPVYLTSGEVVGIAVGSALGLILVFIGCIILRNN 334
[Close]
BLAST of SMED30014212 vs. TrEMBL
Match: A0A4S2LBY7 (Ig-like domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008244 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 3.303e-7
Identity = 74/311 (23.79%), Postives = 126/311 (40.51%), Query Frame = 1 
Query:  118 MSILPK-QNVLQGEKVNISCQFNSYISHLPWSLDTKL----LAENTKSY-------DILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTNAT----------------------------CSSIETGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNT---GELSGGTQKRVNC----PENTKLYKITHTVNINCQHHKLVGRNEIRCQ-SQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKN 906
            ++++P  Q VL G+KV ++CQ   +   L +++  K     ++E  ++Y       +  P +  Q    Y L I +V+ +  G W C   A      L +  P  LK   V  LPF NP K  +   T                               +  G R  PYDL     + + CE+ CG P   +IS    N T +     ++G  + R       P    +   T T+N+NC    L+G NE++C  +   + +    +  Y+LCP       LT GE + I +   +A++ + +   ++R +
Sbjct:   24 LTVIPANQPVLLGQKVILTCQVKGFKRPLDYAVLFKRKDVGISEKCQTYQKNKYHLECEPNNWLQ-ATTYKLYINSVSWADRGDWFCVYAANLTRQHLVVHAPAELKKMAVTSLPF-NPLKSENDRITPVDGSPVLAKLSKSSSSSAVSGAPGQPPAQHVGLGTRTNPYDLRPGLGLQLTCETTCGYP-EANISWLLLNETASRVGTTIAGPNRIRSETCDLSPSTQDMTTTTSTLNVNCVELGLIGLNEVQCAVTGRQYIDHTPTRHVYVLCPGGERPLQLTNGEVVGIAVGAAIALILLFLGCIILRAS 331
[Close]
BLAST of SMED30014212 vs. TrEMBL
Match: A0A267DTQ9 (Ig-like domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029336g1 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.417e-6
Identity = 66/279 (23.66%), Postives = 107/279 (38.35%), Query Frame = 1 
Query:  151 GEKVNISCQFNSY-----ISHLPWSLDTKL-LAENTKSYDILPIDIF------QTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTNATCSSIETGDRRW-----PYDLLNNASVLVLCESECGLPGP------KSISIKYTNSTNTGELS-GGTQKRVNCPENTKLYKITHTVNI--NCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILV--IAFLVRK 903
            G+  N++C   S         + W   T   +AEN  +Y+I   DIF      +   +Y + I+ V+    GLW C     Q    L+++ P +++  +    P   P + +      ++             PYDL     +   C S CG P        + +S++  +  N              C  +  L K T +  +  NC   KL G+   RC   D     I  K  Y+ CPA P    LT GE  A ++  +L +  I++  + F  RK
Sbjct:   51 GQSANLTCHLESAGKQRKTYSVYWHSPTGAEVAENCIAYNIEKHDIFCNNRDAKLATKYTVRIKTVSWKDRGLWYCKFVTNQSYAFLDVNWPATIE--RFTATPVGQPSRDAAAGQQAAAAAAAKAADGSAAAPYDLTLGGGLKFTCRSTCGYPAAQLKLDYRGVSVQLPDPANPNAFRVRSVSDNSTCSSDGNLLKTTESTVLLPNCSALKLDGKTRFRCAIGDSAEPAI-YKDIYVYCPAIPRDYPLTQGEIAAASVGAVLGVFLIILGFVLFCQRK 326
[Close]
BLAST of SMED30014212 vs. TrEMBL
Match: A0A1I8IKW1 (Ig-like domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.516e-6
Identity = 66/290 (22.76%), Postives = 110/290 (37.93%), Query Frame = 1 
Query:  151 GEKVNISCQFNSY-----ISHLPWSLDTKL-LAENTKSYDILPIDIF------QTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTNATCSSIETGDRRW-----PYDLLNNASVLVLCESECGLPGP------KSISIKYTNSTNTGELS-GGTQKRVNCPENTKLYKITHTVNI--NCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILV--IAFLVRKNCGNISDKTDK 936
            G+  N++C   S         + W   T   +AEN  +Y+I   DIF      +   +Y + I+ V+    GLW C     Q    L+++ P +++  +    P   P + +      ++             PYDL     +   C S CG P        + +S++  +  N              C  +  L K T +  +  NC   KL G+   RC   D     I  K  Y+ CPA P    LT GE  A ++  +L +  I++  + F  RK    + +   +
Sbjct:   45 GQSANLTCHLESAGKQRKTYSVYWHSPTGAEVAENCIAYNIEKHDIFCNNRDAKLATKYTVRIKTVSWKDRGLWYCKFVTNQSYAFLDVNWPATIE--RFTATPVGQPSRDAAAGQQAAAAAAAKAADGSAAAPYDLTLGGGLKFTCRSTCGYPAAQLKLDYRGVSVQLPDPANPNAFRVRSVSDNSTCSSDGNLLKTTESTVLLPNCSALKLDGKTRFRCAIGDSAEPAI-YKDIYVYCPAIPRDYPLTQGEIAAASVGAVLGVFLIILGFVLFCQRKPAEELDESASQ 331
[Close]
BLAST of SMED30014212 vs. TrEMBL
Match: A0A5K4EID0 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.931e-6
Identity = 69/312 (22.12%), Postives = 124/312 (39.74%), Query Frame = 1 
Query:  118 MSILPK-QNVLQGEKVNISCQ---------FNSYISHLPWSLDTKLLAENTKSYDIL--PIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKS---SQVKKLPFINPKKL------------------SDTNATCSSIET--------GDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRV------NCPENTKLYKITHTVNINCQHHKLVGRNEIRCQ--SQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKN 906
            +SI+P  Q VL G+KV + CQ         +     H    +  K      + + ++  P D +Q    Y+L I++V+ +  G W+C   A +   TL +  P  L+    S +  LP  N + +                  +  ++  S I T        G R  PYDL +   + + C + CG P   +I+    N T T  +       +      N P    +      ++++C    ++G N+++C      Y   L++ +  YILC        LT GE + I +   L ++ + +   ++R N
Sbjct:   22 LSIIPANQPVLIGQKVILRCQIQGIRLSTKYAVLFRHKETVISEKCFVYQAQKFHLICEPND-WQIAETYELHIKSVSWADRGDWSCIYAANKTKQTLEVYAPAKLEKMGVSSLTSLPVTNKESMIKDSSSPSELSSSSSLATAGLDSALSIIGTPPSQYIGLGTRTNPYDLRSGLGIQLTCATTCGHP-RANITWLVMNETMTKAIMPTNPNIIHENVCSNSPNTIDMTTTESNLHVHCSQLDIIGLNQVQCSVNGPQYKDTLLT-RNVYILCAGGERPMHLTSGEVVGIAVGSALGLILVFIGCIILRNN 330
[Close]
BLAST of SMED30014212 vs. Planmine SMEST
Match: SMESG000064028.1 (SMESG000064028.1)

HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 286/288 (99.31%), Postives = 287/288 (99.65%), Query Frame = 1 
Query:   97 QKNCDCQMSILPKQNVLQGEKVNISCQFNSYISHLPWSLDTKLLAENTKSYDILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTNATCSSIETGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRVNCPENTKLYKITHTVNINCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKNCGNISDKTDKLHCQEIML 960
            QKNCDCQMSILPKQNVLQGEKVNISCQFNSYISHLPWSLDTKLLAENTKSYDILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFI+PKKLSDTNATCSSIETGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRVNC ENTKLYKITHTVNINCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKNCGNISDKTDKLHCQEIML
Sbjct:   16 QKNCDCQMSILPKQNVLQGEKVNISCQFNSYISHLPWSLDTKLLAENTKSYDILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFISPKKLSDTNATCSSIETGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRVNCLENTKLYKITHTVNINCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKNCGNISDKTDKLHCQEIML 303
[Close]
BLAST of SMED30014212 vs. Planmine SMEST
Match: SMESG000078594.1 (SMESG000078594.1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.072e-13
Identity = 74/299 (24.75%), Postives = 127/299 (42.47%), Query Frame = 1 
Query:  115 QMSILPKQNVL-QGEKVNISCQFNSYISHLP--WSLDTKLLAE-----NTKSYDILPIDIFQTGNR---YDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTN--ATCSSIET---------GDRRWPYDLLNNASVLVLCESECGLP-------------GPKSISIKYTNSTNTGELSGGTQKRVNCPENTKLYKITHTVNINCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKN 906
            ++  +P Q+   Q   V +SC+    +S     +  D  + +E     +T+ Y+++  D    G +   Y+  I+ V     G W C  +  + + T++I VP S ++  ++    I P+ +   N     S I++         G R  PY+L    ++ + C S C  P             GP  I +K     N   L          P+   +Y I     INC   K+VG NEI C+S +   + +     Y+LCP       LT GE I + +SV +AI+ I V+  +V+++
Sbjct:   28 EIKKIPDQDYYEQNTTVILSCEIQELLSTFVTMFRKDKNMRSEGCRVFDTQKYEMVCSDKVIDGKKFIKYEFKIKKVEWLDHGEWYCFYSNYRKSVTIDIKVPASFEALNIEP---IKPEGIKSNNLGPDFSPIDSKGNIIVRGRGSRTNPYNLRTGIALKIKCYSSCSYPRIVHSWSYANMSHGPIDIEVKNQRERNICIL----------PKTEGMYSIESEAVINCTALKMVGANEIVCRSAESKKSNV-----YVLCPGGERAWELTTGEIIGLAVSVGIAILLICVLCLMVKRS 308
[Close]
BLAST of SMED30014212 vs. Planmine SMEST
Match: SMESG000056440.1 (SMESG000056440.1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.249e-9
Identity = 71/255 (27.84%), Postives = 108/255 (42.35%), Query Frame = 1 
Query:  193 SHLPWSLDTKLLAENTKSYDILPIDIF-QTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINP--KKLSDTNATCSSIET-----------GDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELS---GGTQKRVNCPENTKLYKITHTVNINCQHHKLVGRNEIRCQSQ-DYHGNL-ISNKPFYILCPAFPGMDF-LTLGEKIAITISVLLAIVDILVIAFLV 897
            S L +S    L++E  K Y+     I  + G  Y   I+ ++   +G W C       +  L+I VP ++  S       I P  KK+  +N    SI +           G +  PYDL  N SV ++C+S+C  P    + I    S  + ELS     T+      E  K   I+  +  NCQ  KL G   + C +  D H  L IS +  YILCP  P   + +T GE I   I   +  V ++ +  ++
Sbjct:   52 SPLLFSRKDDLVSEKCKVYNKSQYGITCKNGTEYKFEIKKMSWIDKGKWKCMVGLYSTSIMLDIYVPSNVSIS-------IEPISKKVFSSNLGPESIPSSHENSIILKGRGQKLNPYDLRKNISVNLICKSDCSYPVVNQVYIFKNFSNFSKELSKKPDNTEINTCDIEQNKTSTISR-LKFNCQAFKLTGLINLTCSTFIDKHSILNISQRTIYILCPTEPIQWWKMTNGEMINTVIGCFMGFVLLMFLFLMI 298
[Close]
BLAST of SMED30014212 vs. Planmine SMEST
Match: SMESG000056440.1 (SMESG000056440.1)

HSP 1 Score: 53.1434 bits (126), Expect = 8.905e-8
Identity = 56/203 (27.59%), Postives = 89/203 (43.84%), Query Frame = 1 
Query:  325 EGLWACGPNAVQFNGTLNISVP--WSLKSSQVKKLPF---INPKKLSDTNATCSSIE-TGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELS---GGTQKRVNCPENTKLYKITHTVNINCQHHKLVGRNEIRCQSQ-DYHGNL-ISNKPFYILCPAFPGMDF-LTLGEKIAITISVLLAIVDILVIAFLV 897
            +G W C       +  L+I VP   S+    + K  F   + P+ +  ++     ++  G +  PYDL  N SV ++C+S+C  P    + I    S  + ELS     T+      E  K   I+  +  NCQ  KL G   + C +  D H  L IS +  YILCP  P   + +T GE I   I   +  V ++ +  ++
Sbjct:    6 KGKWKCMVGLYSTSIMLDIYVPSNVSISIEPISKKVFSSNLGPESIPSSHENSIILKGRGQKLNPYDLRKNISVNLICKSDCSYPVVNQVYIFKNFSNFSKELSKKPDNTEINTCDIEQNKTSTISR-LKFNCQAFKLTGLINLTCSTFIDKHSILNISQRTIYILCPTEPIQWWKMTNGEMINTVIGCFMGFVLLMFLFLMI 207
[Close]
BLAST of SMED30014212 vs. Planmine SMEST
Match: SMESG000003800.1 (SMESG000003800.1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.215e-7
Identity = 67/273 (24.54%), Postives = 114/273 (41.76%), Query Frame = 1 
Query:  151 GEKVNISCQF-NSYISHLPWSLDTKLLAENTKSYD---ILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPNAVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTNATCSSIE-----------TGDRRWPYDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRVNCPENTKL---YKITHTVNINCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAFPGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKNCGN 915
            GE V I C   N     + +    K +AEN  SY+   I+     +    Y L I+ +  ++ G W C          ++I VP +++       P     +L  T+   S +E            G +  P+DL  +A + + C++ C  P     S  Y N T +  +   T   ++    +     Y     + I C+  ++VG NE+ C+  DY+     ++  Y+LCP       LT GE I ++I+  LA + +LV+ F+  K   N
Sbjct:   37 GESVKIKCIVQNVKDLSVLFRHKEKNIAENGHSYNKEFIVKQYPDKQSTIYTLEIKELRWAHNGEWDCVQGLSTRKLVISIIVPGTVE-------PIKPELELPATDKRRSQMELSNHLPQILLGKGIKTNPFDLTRDARIKLYCKTGCSNPQSHH-SWMYNNITMSKRIINATSVSLSSCSLSLAEDGYFTESVILIGCREFQMVGSNEVTCKINDYN----DSQSVYVLCPGGHRPWTLTNGEIIGLSIAFALAAL-MLVLFFMSAKKIIN 296
The following BLAST results are available for this feature:
BLAST of SMED30014212 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2DIS92.848e-1023.20Uncharacterized protein OS=Schistosoma japonicum O... [more]
A0A4S2LBY73.303e-723.79Ig-like domain-containing protein OS=Opisthorchis ... [more]
A0A267DTQ91.417e-623.66Ig-like domain-containing protein (Fragment) OS=Ma... [more]
A0A1I8IKW11.516e-622.76Ig-like domain-containing protein OS=Macrostomum l... [more]
A0A5K4EID01.931e-622.12Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of SMED30014212 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014212 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000064028.10.000e+099.31SMESG000064028.1[more]
SMESG000078594.12.072e-1324.75SMESG000078594.1[more]
SMESG000056440.11.249e-927.84SMESG000056440.1[more]
SMESG000056440.18.905e-827.59SMESG000056440.1[more]
SMESG000003800.11.215e-724.54SMESG000003800.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014212 ID=SMED30014212|Name=SMED30014212|organism=Schmidtea mediterranea sexual|type=transcript|length=1293bp
TGACAATTAATTTTATTTATATATGATTTTAATTCAAATCTACTTGACCA
AATGATTATTTTTTTATCAGTAAACATTCTGATAATTATTTCTTTACAAA
AAAACTGTGATTGCCAAATGTCAATTTTACCGAAACAAAATGTTTTACAG
GGTGAAAAAGTCAATATTTCATGTCAATTTAATTCGTACATAAGTCATTT
GCCATGGAGTTTGGATACAAAGCTTTTAGCAGAAAATACTAAAAGTTATG
ACATATTACCTATTGATATTTTTCAAACTGGAAATAGATATGATTTAATA
ATTAGAAATGTCACTCTTAGCTATGAAGGTTTATGGGCTTGTGGACCTAA
TGCAGTTCAATTTAACGGAACTTTAAACATTTCAGTTCCATGGAGTTTGA
AGTCCTCACAAGTAAAGAAATTACCGTTTATAAATCCGAAGAAACTTTCT
GACACGAATGCCACTTGCAGTAGTATAGAGACTGGTGATAGGAGGTGGCC
ATATGATTTACTGAATAATGCCAGTGTGCTGGTGCTCTGTGAAAGCGAGT
GCGGCCTGCCTGGTCCGAAATCGATATCGATCAAATACACAAATTCGACC
AATACTGGAGAATTGTCAGGTGGAACTCAGAAACGTGTAAATTGTCCTGA
AAACACAAAACTTTACAAAATAACTCACACAGTGAATATTAATTGTCAGC
ATCATAAACTGGTGGGTCGCAACGAAATTCGTTGTCAATCTCAAGATTAC
CACGGAAATCTAATTTCAAATAAACCTTTCTATATTCTTTGTCCAGCATT
TCCCGGAATGGATTTTTTAACTCTCGGTGAAAAGATCGCTATTACTATCT
CAGTTCTGTTGGCCATTGTTGATATCTTAGTTATAGCATTTTTAGTGAGA
AAAAACTGTGGAAATATATCAGATAAAACAGATAAATTACATTGCCAAGA
AATTATGCTCTGATTTCATTATTAAAATTTTGTAAAAATTATATTTGTAA
TAAAGTTCGTTTTTTCTTATAATTTACAATCGGAACCAGTGAACCAGTGT
TGTTATGCGCCCGATAAACGGTTGTGTGTTACTCAATCCGTCGTTATAAA
GTTCACTAATACTAATCTTTGTTTTAATTGTGGTTTTTTTAAATTTTGAC
ACTATTTGACATTGGCATCATCATTGGCAAGTCATTGTTGTTATCTCGTT
GTTTCAGATTTCTCAATGGCAAATGATTTTCACTGACTGAAAATAATAAA
TTTTTTAGTATTTCACCTCGAATAAAACAAAGTTCAATTTGCT
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protein sequence of SMED30014212-orf-1

>SMED30014212-orf-1 ID=SMED30014212-orf-1|Name=SMED30014212-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=304bp
MIIFLSVNILIIISLQKNCDCQMSILPKQNVLQGEKVNISCQFNSYISHL
PWSLDTKLLAENTKSYDILPIDIFQTGNRYDLIIRNVTLSYEGLWACGPN
AVQFNGTLNISVPWSLKSSQVKKLPFINPKKLSDTNATCSSIETGDRRWP
YDLLNNASVLVLCESECGLPGPKSISIKYTNSTNTGELSGGTQKRVNCPE
NTKLYKITHTVNINCQHHKLVGRNEIRCQSQDYHGNLISNKPFYILCPAF
PGMDFLTLGEKIAITISVLLAIVDILVIAFLVRKNCGNISDKTDKLHCQE
IML*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 23..121
e-value: 3.1E-6
score: 29.0
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..21
score: 0.591
NoneNo IPR availableTMHMMTMhelixcoord: 263..282
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 24..97