SMED30014183

Overview
NameSMED30014183
Smed IDSMED30014183
Length (bp)1584
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014183 (SMED30014183) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014183 (SMED30014183) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014183 (SMED30014183) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014183 (SMED30014183) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014183

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30014183SMESG000061464.1 Contig47816newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30014183SMESG000061464.1 Contig47816uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30014183SMESG000061464.1 dd_Smed_v4_21634_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ventral epidermisSMED30014183SMESG000061464.1 dd_Smed_v4_21634_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30014183SMESG000061464.1 dd_Smed_v4_21634_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30014183 vs. Planmine SMEST
Match: SMESG000061464.1 (SMESG000061464.1)

HSP 1 Score: 808.52 bits (2087), Expect = 0.000e+0
Identity = 398/400 (99.50%), Postives = 399/400 (99.75%), Query Frame = 3 
Query:  102 MLFILLLSSVLMHHFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFKMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 1301
            MLFILLLSSVLMH FETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETF+MVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ
Sbjct:    1 MLFILLLSSVLMHQFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFEMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 400

HSP 2 Score: 129.413 bits (324), Expect = 4.782e-32
Identity = 77/95 (81.05%), Postives = 77/95 (81.05%), Query Frame = 1 
Query: 1300 KVTYGIYQAGSGENIFSEYPVIYLAVGAXXXXXXXXXXXXXXXFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 1584
            KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFT            I     I NKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR
Sbjct:  410 KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFT------------IGTIIVIVNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 492
[Close]
BLAST of SMED30014183 vs. Planmine SMEST
Match: SMESG000061464.1 (SMESG000061464.1)

HSP 1 Score: 807.364 bits (2084), Expect = 0.000e+0
Identity = 398/400 (99.50%), Postives = 399/400 (99.75%), Query Frame = 3 
Query:  102 MLFILLLSSVLMHHFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFKMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 1301
            MLFILLLSSVLMH FETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETF+MVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ
Sbjct:    1 MLFILLLSSVLMHQFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFEMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 400

HSP 2 Score: 163.696 bits (413), Expect = 3.235e-44
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 1 
Query: 1300 KVTYGIYQAGSGENIFSEYPVIYLAVGAXXXXXXXXXXXXXXXFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 1584
            KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFTIGTIIVIVFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR
Sbjct:  410 KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFTIGTIIVIVFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 504
[Close]
BLAST of SMED30014183 vs. Planmine SMEST
Match: SMESG000061464.1 (SMESG000061464.1)

HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0
Identity = 387/389 (99.49%), Postives = 388/389 (99.74%), Query Frame = 3 
Query:  135 MHHFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFKMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 1301
            MH FETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETF+MVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ
Sbjct:    1 MHQFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFEMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 389

HSP 2 Score: 129.028 bits (323), Expect = 5.418e-32
Identity = 77/95 (81.05%), Postives = 77/95 (81.05%), Query Frame = 1 
Query: 1300 KVTYGIYQAGSGENIFSEYPVIYLAVGAXXXXXXXXXXXXXXXFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 1584
            KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFT            I     I NKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR
Sbjct:  399 KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFT------------IGTIIVIVNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 481
[Close]
BLAST of SMED30014183 vs. Planmine SMEST
Match: SMESG000061464.1 (SMESG000061464.1)

HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0
Identity = 387/389 (99.49%), Postives = 388/389 (99.74%), Query Frame = 3 
Query:  135 MHHFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFKMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 1301
            MH FETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETF+MVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ
Sbjct:    1 MHQFETREILVKDLSSISYLESIFLNNLSSFPEDFIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFEMVPLCDCFETFRNGDFCNSTHMKTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ 389

HSP 2 Score: 163.31 bits (412), Expect = 3.988e-44
Identity = 95/95 (100.00%), Postives = 95/95 (100.00%), Query Frame = 1 
Query: 1300 KVTYGIYQAGSGENIFSEYPVIYLAVGAXXXXXXXXXXXXXXXFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 1584
            KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFTIGTIIVIVFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR
Sbjct:  399 KVTYGIYQAGSGENIFSEYPVIYLAVGAFIGILFTIGTIIVIVFKCQICIPRCIRNKLQRKNPQNDEFPKFEGSVQLRNGTSYSLLSINPNRMSR 493
[Close]
BLAST of SMED30014183 vs. Planmine SMEST
Match: SMESG000060556.1 (SMESG000060556.1)

HSP 1 Score: 167.933 bits (424), Expect = 5.714e-46
Identity = 109/349 (31.23%), Postives = 181/349 (51.86%), Query Frame = 3 
Query:  237 FIFIIHLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCNCELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYP-VPNFNDHKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKEFGTGISGKCSRFCNRNVCNGGKCLETFKMVPLCDCFETFRNGDFCNS---THMKTDMK--NDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVDKISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITLPVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKW 1265
            F F + L R+ G L Y  D+  NQLI+ + D+ +  + IEF  K++ ++L +LC C  C +  +     + + IN QCN+       +S  +   + YP V  F      N  A LW++NTP    +   FYP+   + GCI  ++ N +  +M+  G  IS +C + C  +VCN G C ET+   P CDC ++F NG FCNS   T M ++ K  ++  + +++K SW ++   IL +  + + L ++++ N L F Y +K  FI+K I      Y  ++     + L F++  I +K  Q+ S     LPV + L+KE+F +I+ L  S     +IV K+   + +W
Sbjct:   32 FEFKMTLNRMEGTLLYMYDKYDNQLIISLSDDLEERMKIEFRGKEHHLTLYELCGCVRCRYRLIDLTVSITDRINIQCNNQPYKIFSYSNQKLINNAYPIVMTF-----ENSQAKLWLVNTP----AHGYFYPNPSQLAGCIHDVIINGVPSEMERSGIEISTECPKACVNDVCNAGVCHETYDREPFCDCTDSFLNGPFCNSSSITQMDSNRKYHSNTFRSKLIKTSWRAHSDDILGIWKEKISLKIQVKRNHLYFLYEEKFLFIKKTIT---NKYQPNQND--CVYLSFDTNFIGIKFVQNDSTIITKLPVPKDLSKEDFTSISLL--SSPGFIDIVHKLRPSQYQW 364
The following BLAST results are available for this feature:
BLAST of SMED30014183 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014183 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061464.14.782e-3299.50SMESG000061464.1[more]
SMESG000061464.13.235e-4499.50SMESG000061464.1[more]
SMESG000061464.15.418e-3299.49SMESG000061464.1[more]
SMESG000061464.13.988e-4499.49SMESG000061464.1[more]
SMESG000060556.15.714e-4631.23SMESG000060556.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014183 ID=SMED30014183|Name=SMED30014183|organism=Schmidtea mediterranea sexual|type=transcript|length=1584bp
TTTTTCTACATCGATATGTAAACAGGTTTATGATCGGTTTGCTTTACATC
ATTTTGGAAAGAGGATTTCAGAAAATAATTATTCCTACCTCAGAAAACTA
AATGCTCTTCATATTGCTTTTATCATCAGTACTTATGCATCATTTCGAAA
CCCGAGAAATTTTAGTCAAAGATTTATCAAGCATTTCTTATTTAGAAAGT
ATATTTTTGAATAATTTATCAAGCTTCCCAGAAGATTTCATCTTCATCAT
CCATTTGACGAGAGTTCCAGGATTACTGTTCTACAGTATTGATAGAAATT
TTAATCAATTAATTGTGAAAATCCATGATGAGTTTGATCCGTTTATTACG
ATAGAATTCAACTTGAAAAAATATTTGATCTCATTGAAAAAATTATGCAA
TTGCGAATTATGTTTGCATGAGAAATTGAAATTTCGGTTTCTCTTAAACA
ATGGAATTAATGCCCAGTGCAACGATGGGGAAATATCAAATCACAAATTC
TCACCCAATCAGCAGTTCCAATCAAGATATCCAGTCCCAAATTTCAACGA
TCATAAAAATTCCAATATATCAGCCTTTCTATGGATGATCAACACCCCAG
AATTTGAAATCTCTGGTTCAAAGTTTTATCCAGATAATGAATACATAACG
GGTTGCATTTTCCAAATCCTTCATAACAATATTTCCGTTAAAATGAAAGA
ATTTGGAACCGGAATATCAGGAAAATGTTCGAGATTTTGTAACAGAAACG
TGTGCAATGGAGGAAAGTGTCTTGAAACATTTAAAATGGTACCTTTATGT
GACTGTTTTGAAACCTTCAGAAACGGAGACTTTTGCAATTCTACTCATAT
GAAGACTGACATGAAAAATGATGGAATTCAGTTTGAAGTTTTAAAAATAT
CATGGAATAGTTATTTAAAACAGATATTGTCTTTAAAAAATAAATATTTA
GAATTGGTTGTAGAAATAGAAGAAAATCAGTTATGTTTCAAATACGTAGA
TAAAATATCCTTCATAAGAAAATATATTAAAATTTTTCCTGGTTATTATT
TAACAGATAAAGAATATATTTTAATAATAACATTAGAATTTGAATCAATA
AAAATACAGGTAAAATTGCACCAAAGCACTTCCTGGACGTCAATAACTCT
GCCGGTTAATCGCACCCTGAATAAGGAGAATTTTAATAACATTACATTTC
TGAATAACTCGTATACAGAACTTGGAGAAATCGTGTTCAAAGTGTCGAAG
ATCGAAAAGAAGTGGACGTATATTGGAAATAGTATTACCAAAACAACTCA
AAGTTACTTACGGAATATATCAAGCAGGTTCGGGAGAAAATATATTTTCA
GAATATCCTGTTATTTATTTGGCTGTTGGAGCATTCATTGGAATATTATT
CACAATTGGGACGATTATTGTTATAGTATTTAAATGCCAAATATGTATTC
CTAGATGTATAAGAAATAAACTTCAAAGGAAAAACCCTCAAAATGATGAA
TTTCCAAAATTTGAGGGAAGTGTTCAACTTCGAAATGGAACGAGCTACTC
TTTACTCAGTATTAATCCAAATCGAATGAGCAGG
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protein sequence of SMED30014183-orf-1

>SMED30014183-orf-1 ID=SMED30014183-orf-1|Name=SMED30014183-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=444bp
MLFILLLSSVLMHHFETREILVKDLSSISYLESIFLNNLSSFPEDFIFII
HLTRVPGLLFYSIDRNFNQLIVKIHDEFDPFITIEFNLKKYLISLKKLCN
CELCLHEKLKFRFLLNNGINAQCNDGEISNHKFSPNQQFQSRYPVPNFND
HKNSNISAFLWMINTPEFEISGSKFYPDNEYITGCIFQILHNNISVKMKE
FGTGISGKCSRFCNRNVCNGGKCLETFKMVPLCDCFETFRNGDFCNSTHM
KTDMKNDGIQFEVLKISWNSYLKQILSLKNKYLELVVEIEENQLCFKYVD
KISFIRKYIKIFPGYYLTDKEYILIITLEFESIKIQVKLHQSTSWTSITL
PVNRTLNKENFNNITFLNNSYTELGEIVFKVSKIEKKWTYIGNSITKTTQ
SYLRNISSRFGRKYIFRISCYLFGCWSIHWNIIHNWDDYCYSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000034epidermis
PLANA:0000096ventral epidermis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.605