Calbindin-32

Overview
NameCalbindin-32
Smed IDSMED30014166
Length (bp)1169
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calbindin-32 (SMED30014166) t-SNE clustered cells

Violin plots show distribution of expression levels for Calbindin-32 (SMED30014166) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calbindin-32 (SMED30014166) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calbindin-32 (SMED30014166) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014166

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30014166h1SMcG0013341 dd_Smed_v4_11287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 183.726 bits (465), Expect = 6.090e-55
Identity = 122/287 (42.51%), Postives = 176/287 (61.32%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++    +   K+ + DL+++L  +  M
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSL---AEAGKLYRKDLEIVLCSEPPM 271
[Close]
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 183.726 bits (465), Expect = 6.090e-55
Identity = 122/287 (42.51%), Postives = 176/287 (61.32%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++    +   K+ + DL+++L  +  M
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSL---AEAGKLYRKDLEIVLCSEPPM 271
[Close]
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB1 (calbindin 1 [Source:HGNC Symbol;Acc:HGNC:1434])

HSP 1 Score: 159.073 bits (401), Expect = 1.466e-45
Identity = 112/279 (40.14%), Postives = 161/279 (57.71%), Query Frame = 3 
Query:  177 FTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
             TA++F E+W H+D DG+GY+EG EL   ++E                        + ++  +   +       ++ Y + +DG+I I ELA +LP EENFLLLFR   QL+S  +F+K WR +D + SGFIE  ELK FLK LL++A  T D  K  + EYT ++L+LFD N DGKL+L+EMARLLPV+ENFL +       ++   E  K F +YDQD +G I+  ELD  LKDL E  +++ D +++   KK ++     S   K+ + DL LIL 
Sbjct:   11 ITASQFFEIWLHFDADGSGYLEGKELQNLIQEL-----------------------QQARKKAGLELSPEMKTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLFRC-QQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTK--LAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKF---QGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNITTYKKNIMAL---SDGGKLYRTDLALILC 257

HSP 2 Score: 71.2478 bits (173), Expect = 1.888e-13
Identity = 64/208 (30.77%), Postives = 109/208 (52.40%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            NFL  FR Q  K     +  EF + W  YD D +G+IE +EL  FLK+ +                      +++K + +T +    + +++ +D NNDG++E+ E+A++LP++ENFLL F+         +F K + ++D++ +G+I+ NEL   LK L ++ K  +D     I  Y K I+ L D    GKL  +++A +L   +N
Sbjct:   87 NFLLLFRCQQLK-----SCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLE---------------------KANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKM--CGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNK--QDLDINNITTYKKNIMALSD---GGKLYRTDLALILCAGDN 261

HSP 3 Score: 68.1662 bits (165), Expect = 2.367e-12
Identity = 56/177 (31.64%), Postives = 91/177 (51.41%), Query Frame = 3 
Query:  501 NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPT--EDTPKEK--IDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            + L ++  F ++W  FD + SG++E  EL+  ++ L +  K    E +P+ K  +D+Y +          DGK+ + E+A +LP  ENFL   +F+     +  E  K +  YD DHSG IE EEL  FLKDL+E   +  DD  L      +L  ++ + D K+   ++  +L  Q
Sbjct:    8 SSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDQYGQ--------RDDGKIGIVELAHVLPTEENFLL--LFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQ 174
[Close]
BLAST of Calbindin-32 vs. Ensembl Human
Match: SCGN (secretagogin, EF-hand calcium binding protein [Source:HGNC Symbol;Acc:HGNC:16941])

HSP 1 Score: 158.688 bits (400), Expect = 2.664e-45
Identity = 108/287 (37.63%), Postives = 158/287 (55.05%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKA----LKNEIMEAYDENNDGRIEIYELAQI-LPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            L    A  F +VW  +D D  GYIE  ELD F    +  + +                        +T MKA    +K + M   D + DGRI + ELA + L  +ENFLLLFRR+N L+SSV+F+++WR +D + SGFI A EL+ FL+ L    K      + K++EYT  ++++FD NKDG+L L+++AR+L ++ENFL +      S   R  + EK+F  YD   +G +E  E+DGF+KD+ME+VQ      DL   ++ LL   + + D KI K +L L L
Sbjct:    9 LGRLDAAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMKLGT-----------------------DDTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLLLFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHKKA--ISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 270

HSP 2 Score: 85.8853 bits (211), Expect = 2.049e-18
Identity = 66/219 (30.14%), Postives = 114/219 (52.05%), Query Frame = 3 
Query:  117 NKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDEL-----DLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            ++D NFL  FR +        ++ EF ++W  YD D +G+I   EL     DLFL                             K +SE  ++     +M+ +D N DGR+++ +LA+IL ++ENFLL F+ D  +  E   DF K++  +D +++G +E  E+  F+K +++  +P+       +D++ +I+L+  D NKDGK+Q SE+A  L ++ N
Sbjct:   89 SEDENFLLLFRRENPLD----SSVEFMQIWRKYDADSSGFISAAELRNFLRDLFL--------------------------HHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQFKMDACSTEERKRDFEKIFAYYDVSKTGALEGPEVDGFVKDMMELVQPS--ISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 275

HSP 3 Score: 54.299 bits (129), Expect = 1.221e-7
Identity = 49/176 (27.84%), Postives = 93/176 (52.84%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL-LPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT-QQNRMYQ 1034
            +  F +VW+ FD ++ G+IE  EL  F  H+L +    +   K  + +  +  +   D +KDG++++ E+A + L   ENFL   +F+  + + +  E  +++  YD D SG I   EL  FL+DL    ++   +  L+    T++  ++++ D ++  +DL  IL  Q+N + Q
Sbjct:   14 AAGFWQVWQRFDADEKGYIEEKELDAFFLHMLMKLGTDDTVMKANLHKVKQQFMTTQDASKDGRIRMKELAGMFLSEDENFLL--LFRRENPLDSSVEFMQIWRKYDADSSGFISAAELRNFLRDLFLHHKKAISEAKLEEYTGTMMKIFDRNKDGRLDLNDLARILALQENFLLQ 187
[Close]
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 156.377 bits (394), Expect = 7.377e-45
Identity = 105/245 (42.86%), Postives = 151/245 (61.63%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQ 902
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD++    +  ++L  + K +M + +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKE----MNIQQLTNYRKSVMSLAE 228

HSP 2 Score: 79.7221 bits (195), Expect = 1.807e-16
Identity = 56/170 (32.94%), Postives = 94/170 (55.29%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPK-EKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            ++  F+++W+ FD + +G+IE  EL+ F + L K  K +    K +   E  K  +Q +D N DGK++++E+A++LP  ENFL    F+     +  E  + +  YD D SG IE  EL GFL DL++     YD+  LQ   +T+L  ++ + D K+   ++  +L  Q
Sbjct:   17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183
[Close]
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0340072)

HSP 1 Score: 279.256 bits (713), Expect = 6.360e-92
Identity = 159/298 (53.36%), Postives = 221/298 (74.16%), Query Frame = 3 
Query:  120 KDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIV-QEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            K  NF+ Q+RD  +++L+  +A +F +VW HYDKDGNGYIEG ELD FL+EFVSS  + +                S + +++T ++ LK+  MEAYD+N DG+I+I ELAQ+LPMEENFLLLFR DN LESSV+F+K+WR +D + SG+IEA+ELK FL+ LLKEAK   D  ++K+ EYT  +LQ+FD NKDG+LQLSEMA+LLPV+ENFLCR +FK A+++T+ +IEK+F +YD+D+SGTIENEEL GFLKDL+E+V +++YD  DL   ++T++         KI++ +L +IL
Sbjct:   16 KAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDI---------------SPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMIL 298
[Close]
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0087056)

HSP 1 Score: 279.256 bits (713), Expect = 6.360e-92
Identity = 159/298 (53.36%), Postives = 221/298 (74.16%), Query Frame = 3 
Query:  120 KDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIV-QEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            K  NF+ Q+RD  +++L+  +A +F +VW HYDKDGNGYIEG ELD FL+EFVSS  + +                S + +++T ++ LK+  MEAYD+N DG+I+I ELAQ+LPMEENFLLLFR DN LESSV+F+K+WR +D + SG+IEA+ELK FL+ LLKEAK   D  ++K+ EYT  +LQ+FD NKDG+LQLSEMA+LLPV+ENFLCR +FK A+++T+ +IEK+F +YD+D+SGTIENEEL GFLKDL+E+V +++YD  DL   ++T++         KI++ +L +IL
Sbjct:   16 KAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDI---------------SPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMIL 298
[Close]
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010])

HSP 1 Score: 181.8 bits (460), Expect = 2.529e-54
Identity = 110/286 (38.46%), Postives = 170/286 (59.44%), Query Frame = 3 
Query:  174 NFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELA-QILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNR 1025
            N  A  F ++W H+D D NGYIEG ELD F +  +  +                   +    +++  ++ +K   M AYD   DGR++I ELA  ILP EENFLL+FRR+  L++SV+F+K+WR +D + SG+I A ELK FLK L  +    +  P  K+DEYT  ++++FD NKDG+L L+++AR+L ++ENFL +     +S++ R  + EK+F  YD   +G +E  E+DGF+KD+ME+V+      DL   ++ LL   + + D KI K +L L L  +++
Sbjct:    7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHMLKKL-------------------QPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQH--KKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKHK 271

HSP 2 Score: 83.5741 bits (205), Expect = 8.369e-18
Identity = 65/214 (30.37%), Postives = 111/214 (51.87%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDEL-----DLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRE 749
            NFL  FR +    L N  + EF ++W  YD D +GYI   EL     DLFL                          +  K +    +    + +M+ +D+N DGR+++ +LA+IL ++ENFLL F+ D  +Q+E   DF K++  +D +++G +E  E+  F+K +++  +P+       +D++ + +L   D NKDGK+Q SE+A  L ++ 
Sbjct:   89 NFLLIFRREAP--LDN--SVEFMKIWRKYDADSSGYISAAELKNFLKDLFL--------------------------QHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPS--ISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270

HSP 3 Score: 70.8626 bits (172), Expect = 2.311e-13
Identity = 56/176 (31.82%), Postives = 98/176 (55.68%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL-LPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT-QQNRMYQ 1034
            +  F+++W+ FD + +G+IE  EL +F +H+LK+ +P +    E++ +  K  +  +D   DG+LQ+ E+A + LP  ENFL   IF+  + +    E  K++  YD D SG I   EL  FLKDL    +++   + L      ++  ++K+ D ++  +DL  IL  Q+N + Q
Sbjct:   10 AAGFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQ 183
[Close]
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010])

HSP 1 Score: 181.8 bits (460), Expect = 2.529e-54
Identity = 110/286 (38.46%), Postives = 170/286 (59.44%), Query Frame = 3 
Query:  174 NFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELA-QILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNR 1025
            N  A  F ++W H+D D NGYIEG ELD F +  +  +                   +    +++  ++ +K   M AYD   DGR++I ELA  ILP EENFLL+FRR+  L++SV+F+K+WR +D + SG+I A ELK FLK L  +    +  P  K+DEYT  ++++FD NKDG+L L+++AR+L ++ENFL +     +S++ R  + EK+F  YD   +G +E  E+DGF+KD+ME+V+      DL   ++ LL   + + D KI K +L L L  +++
Sbjct:    7 NLDAAGFLQIWQHFDADDNGYIEGKELDDFFRHMLKKL-------------------QPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQH--KKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPSISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKHK 271

HSP 2 Score: 83.5741 bits (205), Expect = 8.369e-18
Identity = 65/214 (30.37%), Postives = 111/214 (51.87%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDEL-----DLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRE 749
            NFL  FR +    L N  + EF ++W  YD D +GYI   EL     DLFL                          +  K +    +    + +M+ +D+N DGR+++ +LA+IL ++ENFLL F+ D  +Q+E   DF K++  +D +++G +E  E+  F+K +++  +P+       +D++ + +L   D NKDGK+Q SE+A  L ++ 
Sbjct:   89 NFLLIFRREAP--LDN--SVEFMKIWRKYDADSSGYISAAELKNFLKDLFL--------------------------QHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPS--ISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270

HSP 3 Score: 70.8626 bits (172), Expect = 2.311e-13
Identity = 56/176 (31.82%), Postives = 98/176 (55.68%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL-LPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT-QQNRMYQ 1034
            +  F+++W+ FD + +G+IE  EL +F +H+LK+ +P +    E++ +  K  +  +D   DG+LQ+ E+A + LP  ENFL   IF+  + +    E  K++  YD D SG I   EL  FLKDL    +++   + L      ++  ++K+ D ++  +DL  IL  Q+N + Q
Sbjct:   10 AAGFLQIWQHFDADDNGYIEGKELDDFFRHMLKKLQPKDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLL--IFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQ 183
[Close]
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2a (calbindin 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1677])

HSP 1 Score: 181.03 bits (458), Expect = 3.931e-54
Identity = 120/283 (42.40%), Postives = 167/283 (59.01%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQ 1016
            L   TA++F ++W H+D DGNGYIEG ELD F  +   +                          S  A K    E M+ +D+N DGRIE+ ELAQILP EENFLL FR+   + SS +F+  WR +D ++SGFIEANELK FL  LLK+A    D   +K+ EYT+ IL++FD N DGKL LSEMARLLPV+ENFL +  F+   R++  +   +F  YD+D +G I+ +ELD  LKDL E   +E D   L   K++++     S   K+ + +L+++L +
Sbjct:   13 LAELTASQFLDIWKHFDADGNGYIEGKELDNFFSQLEIARRGAGVDP------------------SNAAFKGRMTEFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSTEFMAAWRKYDTDRSGFIEANELKGFLSDLLKKANRHYDD--QKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGV-RLSAEQFNAIFTYYDKDGNGYIDEQELDALLKDLYEKNNKEVDSKSLTGYKQSIMAL---SDGGKLYRSELEIVLCR 267

HSP 2 Score: 86.6557 bits (213), Expect = 5.918e-19
Identity = 56/175 (32.00%), Postives = 91/175 (52.00%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEANX-----XXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFL-CRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            ++  F+ +W+ FD + +G+IE  E      +  +        P+    K ++ E+    +Q FD NKDG++++SE+A++LP  ENFL C   F  +S     E    +  YD D SG IE  EL GFL DL++     YDD  LQ   +T+L  ++ + D K+   ++  +L  Q
Sbjct:   17 TASQFLDIWKHFDADGNGYIEGKELDNFFSQLEIARRGAGVDPSNAAFKGRMTEF----MQKFDKNKDGRIEMSELAQILPTEENFLLCFRQFVGSST----EFMAAWRKYDTDRSGFIEANELKGFLSDLLKKANRHYDDQKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQ 183

HSP 3 Score: 57.7658 bits (138), Expect = 4.440e-9
Identity = 40/149 (26.85%), Postives = 74/149 (49.66%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIE 575
            NFL  FR          ++TEF   W  YD D +G+IE +EL  FL + +                      ++++   +  ++     I++ +D N DG++ + E+A++LP++ENFLL F+      S+  F  ++  +DK+ +G+I+
Sbjct:   97 NFLLCFRQFVG------SSTEFMAAWRKYDTDRSGFIEANELKGFLSDLLK---------------------KANRHYDDQKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQ--GVRLSAEQFNAIFTYYDKDGNGYID 216
[Close]
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2b (calbindin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1668])

HSP 1 Score: 170.629 bits (431), Expect = 3.505e-50
Identity = 117/283 (41.34%), Postives = 168/283 (59.36%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQ 1016
            L   TA++F ++WN +D DGNG IEG EL+ F++E                          +   S  + K    E M+ +DEN DG+IE+ ELAQILP EENFLL FR+   + SS +F+  WR +D ++SG+IE+NELK FL  LLK+A    D   +K++EYT+ IL++FD N DGKL LSEMARLLPV ENFL +  F+N  +++  E E +F  YD+D +G I+  ELD  L+DL    +   D H L   KK+++     S   K+ + +L+++L +
Sbjct:   13 LAELTASQFLDIWNKFDADGNGCIEGKELENFIRELEI--------------------ARKTADPSNASFKDRMKEFMQKFDENGDGKIEMSELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGYIESNELKGFLSDLLKKANRHYDD--KKLNEYTQTILKMFDLNGDGKLGLSEMARLLPVEENFLLK--FQNF-KLSAEEFEAIFNYYDKDGNGYIDEHELDALLRDLYHKHKMAVDPHSLSSSKKSIMAL---SDGGKLFRTELEIVLCK 265
[Close]
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb1 (calbindin 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8107])

HSP 1 Score: 156.762 bits (395), Expect = 5.783e-45
Identity = 116/281 (41.28%), Postives = 167/281 (59.43%), Query Frame = 3 
Query:  180 TATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSET-AMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            +A++F ++++HYD DGNG+IEG EL  F+KE                      Q      L  T  MKA     +E Y++N DG+I I EL QILP EENFLL FR+  QL+S  +F++ WR++D + SG+IEA+ELK FLK LL+ AK   D  ++K++EYT   L++FD NKDGKL LSEMARLLP  ENFL +  F+   ++ R E  K+F +YD+D +G ++  ELD  LKDL E   +  + + +   K  ++     S   K+ + +L L+L  +
Sbjct:   12 SASQFLDIFHHYDNDGNGFIEGKELQNFIKELQ--------------------QARKQAGLELTDKMKAF----VEKYEQNADGKIGIIELVQILPTEENFLLFFRQ--QLKSCAEFMEAWRIYDADHSGYIEADELKNFLKDLLQRAKKPYD--EKKLEEYTLTTLKIFDSNKDGKLCLSEMARLLPDNENFLLK--FQGV-KMARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNNKVLEANKISTYKSAIMAL---SDGGKLYRTELALVLCAE 258

HSP 2 Score: 67.3958 bits (163), Expect = 2.716e-12
Identity = 70/226 (30.97%), Postives = 108/226 (47.79%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRE 749
            N+  +I +  +V     + NFL  FR Q        +  EF E W  YD D +GYIE DEL  FL                K+L +   +    K L E  +  LK      +D N DG++ + E+A++LP  ENFLL F+      +  +F K++ ++DK+++G+++ NEL   LK L ++     +    KI  Y   I+ L D    GKL  +E+A +L   E
Sbjct:   68 NADGKIGIIELVQILPTEENFLLFFRQQLK------SCAEFMEAWRIYDADHSGYIEADELKNFL----------------KDLLQRAKKPYDEKKLEEYTLTTLK-----IFDSNKDGKLCLSEMARLLPDNENFLLKFQGVKM--ARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNNKVLEA--NKISTYKSAIMALSD---GGKLYRTELALVLCAEE 259
[Close]
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242])

HSP 1 Score: 171.4 bits (433), Expect = 3.007e-50
Identity = 101/278 (36.33%), Postives = 160/278 (57.55%), Query Frame = 3 
Query:  183 ATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELA-QILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            A EF E+W  +D D NGYIEG ELD F    +  +                     +  ++   ++ +K+  M AYD   DGR++I ELA  ILP +ENFLLLFRR+  L++SV+F+++WR +D++ SGFI A  ++          +  +    +K+DEYT  +++LF+ NKDG+L L+++A++L ++ENFL +     +S+  R  + E +F  YD   +G +E  E+DGF+KD+ME+V+      DL   ++ LLN  + + D KI K +L L L
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL--------------------GANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISA--VELRNFLQDLFLQHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 265

HSP 2 Score: 78.9518 bits (193), Expect = 4.117e-16
Identity = 67/227 (29.52%), Postives = 120/227 (52.86%), Query Frame = 3 
Query:   93 VCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDEL-----DLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            + N+++   +D NFL  FR +T   L N  + EF  +W  YD+D +G+I   EL     DLFL                          +  K ++   +    + +M+ +++N DGR+++ +LA+IL ++ENFLL F+ D  +Q E   DF  ++  +D +++G +E  E+  F+K +++  KP+       +D++ +I+L   D NKDGK+Q SE+A  L ++ +
Sbjct:   78 LANMILP--EDENFLLLFRRETP--LDN--SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFL--------------------------QHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPS--ISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 270
[Close]
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242])

HSP 1 Score: 162.925 bits (411), Expect = 4.759e-47
Identity = 96/277 (34.66%), Postives = 157/277 (56.68%), Query Frame = 3 
Query:  183 ATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELA-QILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            A EF E+W  +D D NGYIEG ELD F    +  +                     +  ++   ++ +K+  M AYD   DGR++I ELA  ILP +ENFLLLFRR+  L++SV+F+++WR +D++ SGFI A  ++          +  +    +K+DEYT  +++LF+ NKDG+L L+++A+ +   ++FL   ++ ++    + + E +F  YD   +G +E  E+DGF+KD+ME+V+      DL   ++ LLN  + + D KI K +L L L
Sbjct:   10 AAEFLEIWQRFDADDNGYIEGKELDEFFCHLLKKL--------------------GANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISA--VELRNFLQDLFLQHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKYV-FYKDFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDKFRQILLNHCDVNKDGKIQKSELALCL 263

HSP 2 Score: 60.8474 bits (146), Expect = 7.240e-10
Identity = 58/225 (25.78%), Postives = 112/225 (49.78%), Query Frame = 3 
Query:   93 VCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDEL-----DLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            + N+++   +D NFL  FR +T   L N  + EF  +W  YD+D +G+I   EL     DLFL                          +  K ++   +    + +M+ +++N DGR+++ +LA+ +  ++  +      +Q E   DF  ++  +D +++G +E  E+  F+K +++  KP+       +D++ +I+L   D NKDGK+Q SE+A  L ++ +
Sbjct:   78 LANMILP--EDENFLLLFRRETP--LDN--SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFL--------------------------QHKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKYVFYKDFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPS--ISGVDLDKFRQILLNHCDVNKDGKIQKSELALCLGLKPH 268
[Close]
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: gata5 (GATA binding protein 5 [Source:Xenbase;Acc:XB-GENE-484906])

HSP 1 Score: 147.132 bits (370), Expect = 1.012e-41
Identity = 96/199 (48.24%), Postives = 129/199 (64.82%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCR 764
            L   TA++F ++W HYD DGNGYIEG EL+ F +E  ++                  +G      +    + LK E M+ YD+N DGRIE+ ELAQILP EENFLL FR+     SS++F++ WR +D ++SG+IEANELK FL  LLK+A    D  ++K+ EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +
Sbjct:   12 LAELTASQFLDIWKHYDSDGNGYIEGKELENFFRELEAAR-----------------RGAGVDASNVNFGEKLK-EFMQKYDKNADGRIEMAELAQILPTEENFLLCFRQ--HAGSSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPFD--EKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLK 188

HSP 2 Score: 75.0998 bits (183), Expect = 3.392e-15
Identity = 50/170 (29.41%), Postives = 90/170 (52.94%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTE-DTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            ++  F+ +W+ +D + +G+IE  EL+ F + L    +    D       E  K  +Q +D N DG+++++E+A++LP  ENFL    F+  +  +  E  + +  YD D SG IE  EL GFL DL++     +D+  L    +T+L  ++ + D K+   ++  +L  Q
Sbjct:   16 TASQFLDIWKHYDSDGNGYIEGKELENFFRELEAARRGAGVDASNVNFGEKLKEFMQKYDKNADGRIEMAELAQILPTEENFLL--CFRQHAG-SSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPFDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQ 182

HSP 3 Score: 56.6102 bits (135), Expect = 9.457e-9
Identity = 38/141 (26.95%), Postives = 65/141 (46.10%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFR 494
            N+  RI +  +      + NFL  FR          ++ EF E W  YD D +GYIE +EL  FL + +                      ++++   E  +      I+  +D N DG++ + E++++LP++ENFLL F+
Sbjct:   77 NADGRIEMAELAQILPTEENFLLCFRQHAG------SSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---------------------KANRPFDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLKFQ 190
[Close]
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969])

HSP 1 Score: 143.665 bits (361), Expect = 1.007e-39
Identity = 109/258 (42.25%), Postives = 158/258 (61.24%), Query Frame = 3 
Query:  180 TATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXX-XXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLL 950
            +A++F E+W HYD DGNG+IEG EL  F+ E   +                  + ++   LS+  MKA     ++ Y+ N DG+I I ELAQILP EENFLL FR+  QL+SS +F++ WR +D + SGFIE +ELK      L K  KP E+T   K++EYT  +L +FD N DGKL L+EM+ LLPV+ENFL +  F+   ++   E  K+F +YD+D +G I+  ELD  LKDL +  +++ D ++L   KK+++
Sbjct:   17 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQA------------------RKKAGLDLSDQ-MKAF----VDQYENNTDGKIGIAELAQILPTEENFLLFFRQ--QLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEET---KLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLK--FQGV-KMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKDLDINNLSTYKKSIM 243

HSP 2 Score: 73.1738 bits (178), Expect = 4.815e-14
Identity = 52/173 (30.06%), Postives = 90/173 (52.02%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEA----NXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            S+  F+++WR +D + +GFIE     N + E  +   K      D  K  +D+Y        + N DGK+ ++E+A++LP  ENFL   +F      +  E  + +  YD DHSG IE +EL  FLKDL++   +  ++  L+    T+L  ++ + D K+   ++ ++L  Q
Sbjct:   17 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQARKKAGLDLSDQMKAFVDQY--------ENNTDGKIGIAELAQILPTEENFL---LFFRQQLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQ 178

HSP 3 Score: 68.5514 bits (166), Expect = 1.581e-12
Identity = 48/170 (28.24%), Postives = 86/170 (50.59%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEAN 581
            N+  +I +  +      + NFL  FR Q        ++ EF + W  YD D +G+IE DEL  FLK+ +                      +++K   ET ++   + ++  +D NNDG++ + E++ +LP++ENFLL F+         +F KV+ ++DK+ +G+I+ N
Sbjct:   73 NTDGKIGIAELAQILPTEENFLLFFRQQLK------SSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLK---------------------KANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQGVKM--CGKEFNKVFELYDKDGNGYIDEN 213
[Close]
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969])

HSP 1 Score: 139.428 bits (350), Expect = 7.475e-38
Identity = 115/303 (37.95%), Postives = 167/303 (55.12%), Query Frame = 3 
Query:  180 TATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXX-XXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFL-------------------CRPIFK-----NASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
            +A++F E+W HYD DGNG+IEG EL  F+ E   +                  + ++   LS+  MKA     ++ Y+ N DG+I I ELAQILP EENFLL FR+  QL+SS +F++ WR +D + SGFIE +ELK      L K  KP E+T   K++EYT  +L +FD N DGKL L+EM+ LLPV+ENFL                   C  I +        ++   E  K+F +YD+D +G I+  ELD  LKDL +  +++ D ++L   KK+++     S   K+ + +L L+L 
Sbjct:   12 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQA------------------RKKAGLDLSD-QMKAF----VDQYENNTDGKIGIAELAQILPTEENFLLFFRQ--QLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEET---KLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQISVNFICVGINQMCFNPQGVKMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKDLDINNLSTYKKSIMAL---SDGGKLYRTELALVLC 283

HSP 2 Score: 73.1738 bits (178), Expect = 4.834e-14
Identity = 54/183 (29.51%), Postives = 97/183 (53.01%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEA----NXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL-TQQNRMYQSELS 1046
            S+  F+++WR +D + +GFIE     N + E  +   K      D  K  +D+Y        + N DGK+ ++E+A++LP  ENFL   +F      +  E  + +  YD DHSG IE +EL  FLKDL++   +  ++  L+    T+L  ++ + D K+   ++ ++L  Q+N + + ++S
Sbjct:   12 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQARKKAGLDLSDQMKAFVDQY--------ENNTDGKIGIAELAQILPTEENFL---LFFRQQLKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVS 183

HSP 3 Score: 69.3218 bits (168), Expect = 1.039e-12
Identity = 70/233 (30.04%), Postives = 113/233 (48.50%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQ---LESSVDFI----------------------KVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            NFL  FR Q        ++ EF + W  YD D +G+IE DEL  FLK+ +                      +++K   ET ++   + ++  +D NNDG++ + E++ +LP++ENFLL F+  +    L+ SV+FI                      KV+ ++DK+ +G+I+ NEL + LK L  + K  +D     +  Y K I+ L D    GKL  +E+A +L   EN
Sbjct:   87 NFLLFFRQQLK------SSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLK---------------------KANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQISVNFICVGINQMCFNPQGVKMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNK--KDLDINNLSTYKKSIMALSD---GGKLYRTELALVLCADEN 287
[Close]
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914])

HSP 1 Score: 182.57 bits (462), Expect = 1.297e-54
Identity = 121/284 (42.61%), Postives = 175/284 (61.62%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++    +   K+ + DL+++L  +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTTYRKSVMSL---AEAGKLYRKDLEIVLCSE 268
[Close]
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914])

HSP 1 Score: 175.252 bits (443), Expect = 3.636e-52
Identity = 114/242 (47.11%), Postives = 153/242 (63.22%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLME 893
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYE 229

HSP 2 Score: 79.7221 bits (195), Expect = 1.162e-16
Identity = 56/170 (32.94%), Postives = 94/170 (55.29%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPK-EKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            ++  F+++W+ FD + +G+IE  EL+ F + L K  K +    K +   E  K  +Q +D N DGK++++E+A++LP  ENFL    F+     +  E  + +  YD D SG IE  EL GFL DL++     YD+  LQ   +T+L  ++ + D K+   ++  +L  Q
Sbjct:   17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLL--CFRQHVG-SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQ 183
[Close]
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb1 (calbindin 1 [Source:MGI Symbol;Acc:MGI:88248])

HSP 1 Score: 160.999 bits (406), Expect = 1.645e-46
Identity = 115/279 (41.22%), Postives = 165/279 (59.14%), Query Frame = 3 
Query:  177 FTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
             TA++F E+W H+D DG+GY+EG EL   ++E + +                    + + +     MK+     ++ Y + +DG+I I ELA +LP EENFLLLFR   QL+S  +F+K WR +D + SGFIE  ELK FLK LL++A  T D  K  + EYT ++L+LFD N DGKL+L+EMARLLPV+ENFL +       ++   E  K F +YDQD +G I+  ELD  LKDL E  ++E D +++   KK ++     S   K+ + DL LIL+
Sbjct:   11 ITASQFFEIWLHFDADGSGYLEGKELQNLIQELLQAR-------------------KKAGLELSPEMKSF----VDQYGQRDDGKIGIVELAHVLPTEENFLLLFRC-QQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTK--LAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKF---QGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQELDINNITTYKKNIMAL---SDGGKLYRTDLALILS 257

HSP 2 Score: 70.8626 bits (172), Expect = 2.100e-13
Identity = 64/208 (30.77%), Postives = 108/208 (51.92%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            NFL  FR Q  K     +  EF + W  YD D +G+IE +EL  FLK+ +                      +++K + +T +    + +++ +D NNDG++E+ E+A++LP++ENFLL F+         +F K + ++D++ +G+I+ NEL   LK L ++ K   D     I  Y K I+ L D    GKL  +++A +L   +N
Sbjct:   87 NFLLLFRCQQLK-----SCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLE---------------------KANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKM--CGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQELDI--NNITTYKKNIMALSD---GGKLYRTDLALILSAGDN 261

HSP 3 Score: 68.1662 bits (165), Expect = 1.551e-12
Identity = 57/177 (32.20%), Postives = 92/177 (51.98%), Query Frame = 3 
Query:  501 NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPT--EDTPKEK--IDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            + L ++  F ++W  FD + SG++E  EL+  ++ LL+  K    E +P+ K  +D+Y +          DGK+ + E+A +LP  ENFL   +F+     +  E  K +  YD DHSG IE EEL  FLKDL+E   +  DD  L      +L  ++ + D K+   ++  +L  Q
Sbjct:    8 SSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELLQARKKAGLELSPEMKSFVDQYGQ--------RDDGKIGIVELAHVLPTEENFLL--LFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQ 174
[Close]
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873])

HSP 1 Score: 144.821 bits (364), Expect = 3.618e-40
Identity = 95/292 (32.53%), Postives = 159/292 (54.45%), Query Frame = 3 
Query:  147 RDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQI-LPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRIT---RYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            R +T  +L    A  F ++W  +DK+  GYI   ELD F    ++                    G    ++ E   K +K ++M +++ + +GRI + ELA + L  +ENFLL FR +  L++SV+F+++WR +D + SGFI A   +             ++  + +++EYT  ++++FD NKDG+L L+++AR+L ++ENFL +  FK  +  T   + + EK+F  YD   +G +E  E+DGF+KD+ME+VQ      DL   ++ LL   + + D KI K +L L L
Sbjct:    5 RRKTPARLD---AACFWQIWQRFDKEEKGYIRETELDAFFDHLLAK------------------SGTEDTLMEENVQK-VKEQLMTSHNVSKEGRILMKELASMFLSEDENFLLFFRLETPLDNSVEFMQIWRKYDADSSGFISA--AELCNFLRDLFLHHKKNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQ--FKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 270

HSP 2 Score: 85.5001 bits (210), Expect = 2.015e-18
Identity = 69/219 (31.51%), Postives = 118/219 (53.88%), Query Frame = 3 
Query:  117 NKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDEL-----DLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            ++D NFL  FR +T   L N  + EF ++W  YD D +G+I   EL     DLFL                             K +SE  ++   + +M+ +D+N DGR+++ +LA+IL ++ENFLL F+ D  +  E   DF K++  +D +++G +E  E+  F+K +++  +P+       +D++ +I+L+  D NKDGK+Q SE+A  L ++ N
Sbjct:   89 SEDENFLLFFRLETP--LDN--SVEFMQIWRKYDADSSGFISAAELCNFLRDLFL--------------------------HHKKNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPS--ISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 275

HSP 3 Score: 48.9062 bits (115), Expect = 4.555e-6
Identity = 52/174 (29.89%), Postives = 95/174 (54.60%), Query Frame = 3 
Query:  525 FIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDT-PKEKIDEYTKIILQLFDYNKDGKLQLSEMARL-LPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT-QQNRMYQ 1034
            F ++W+ FDK + G+I   EL  F  HLL ++  TEDT  +E + +  + ++   + +K+G++ + E+A + L   ENFL    F+  + +    E  +++  YD D SG I   EL  FL+DL    ++   + +L+    T++  ++K+ D ++  +DL  IL  Q+N + Q
Sbjct:   17 FWQIWQRFDKEEKGYIRETELDAFFDHLLAKS-GTEDTLMEENVQKVKEQLMTSHNVSKEGRILMKELASMFLSEDENFLL--FFRLETPLDNSVEFMQIWRKYDADSSGFISAAELCNFLRDLFLHHKKNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQ 187
[Close]
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873])

HSP 1 Score: 82.0333 bits (201), Expect = 1.078e-18
Identity = 46/115 (40.00%), Postives = 72/115 (62.61%), Query Frame = 3 
Query:  675 LQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRIT---RYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            +++FD NKDG+L L+++AR+L ++ENFL +  FK  +  T   + + EK+F  YD   +G +E  E+DGF+KD+ME+VQ      DL   ++ LL   + + D KI K +L L L
Sbjct:    1 MKIFDKNKDGRLDLNDLARILALQENFLLQ--FKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDKFREILLRHCDVNKDGKIQKSELALCL 113

HSP 2 Score: 67.3958 bits (163), Expect = 2.210e-13
Identity = 43/120 (35.83%), Postives = 78/120 (65.00%), Query Frame = 3 
Query:  399 MEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQL--ESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVREN 752
            M+ +D+N DGR+++ +LA+IL ++ENFLL F+ D     E   DF K++  +D +++G +E  E+  F+K +++  +P+       +D++ +I+L+  D NKDGK+Q SE+A  L ++ N
Sbjct:    1 MKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPS--ISGVDLDKFREILLRHCDVNKDGKIQKSELALCLGLKIN 118
[Close]
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P41044|CAB32_DROME (Calbindin-32 OS=Drosophila melanogaster OX=7227 GN=Cbp53E PE=2 SV=1)

HSP 1 Score: 279.256 bits (713), Expect = 6.375e-91
Identity = 159/298 (53.36%), Postives = 221/298 (74.16%), Query Frame = 3 
Query:  120 KDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIV-QEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            K  NF+ Q+RD  +++L+  +A +F +VW HYDKDGNGYIEG ELD FL+EFVSS  + +                S + +++T ++ LK+  MEAYD+N DG+I+I ELAQ+LPMEENFLLLFR DN LESSV+F+K+WR +D + SG+IEA+ELK FL+ LLKEAK   D  ++K+ EYT  +LQ+FD NKDG+LQLSEMA+LLPV+ENFLCR +FK A+++T+ +IEK+F +YD+D+SGTIENEEL GFLKDL+E+V +++YD  DL   ++T++         KI++ +L +IL
Sbjct:   16 KAHNFMRQYRDPESRELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDI---------------SPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKKDDYDAQDLAAFEETIMRGVGTDKHGKISRKELTMIL 298
[Close]
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P22676|CALB2_HUMAN (Calretinin OS=Homo sapiens OX=9606 GN=CALB2 PE=2 SV=2)

HSP 1 Score: 183.726 bits (465), Expect = 2.925e-54
Identity = 122/287 (42.51%), Postives = 176/287 (61.32%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++    +   K+ + DL+++L  +  M
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMNIQQLTNYRKSVMSL---AEAGKLYRKDLEIVLCSEPPM 271
[Close]
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P47728|CALB2_RAT (Calretinin OS=Rattus norvegicus OX=10116 GN=Calb2 PE=1 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 3.396e-54
Identity = 122/287 (42.51%), Postives = 176/287 (61.32%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++    +   K+ + DL+++L  +  M
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTTYRKSVMSL---AEAGKLYRKDLEIVLCSEPPM 271
[Close]
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q3ZBY3|CALB2_BOVIN (Calretinin OS=Bos taurus OX=9913 GN=CALB2 PE=2 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 8.510e-54
Identity = 124/285 (43.51%), Postives = 177/285 (62.11%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADA-KITKDDLQLILTQQ 1019
            L   TAT+F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++     A+A K+ + DL+++L  +
Sbjct:   13 LAELTATQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMVSKSDNLGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSTEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQGM-KLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTSYRKSVMSL----AEAEKLYRKDLEIVLCSE 268
[Close]
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q08331|CALB2_MOUSE (Calretinin OS=Mus musculus OX=10090 GN=Calb2 PE=1 SV=3)

HSP 1 Score: 182.57 bits (462), Expect = 9.073e-54
Identity = 121/284 (42.61%), Postives = 175/284 (61.62%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            L   TA++F E+W H+D DGNGYIEG EL+ F +E   +                  +G      S+   + +K E M+ YD+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F++ WR +D ++SG+IEANELK FL  LLK+A    D PK  + EYT+ IL++FD N DGKL LSEM+RLLPV+ENFL +  F+   ++T  E   +F  YD+D SG I+  ELD  LKDL E  ++E +   L   +K++++    +   K+ + DL+++L  +
Sbjct:   13 LAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKAR-----------------KGSGMMSKSDNFGEKMK-EFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQ--HVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEPK--LQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK--FQ-GMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEMNIQQLTTYRKSVMSL---AEAGKLYRKDLEIVLCSE 268
[Close]
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GD68 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g2 PE=4 SV=1)

HSP 1 Score: 337.035 bits (863), Expect = 3.861e-111
Identity = 186/316 (58.86%), Postives = 247/316 (78.16%), Query Frame = 3 
Query:  108 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTP--KEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEE-------YDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            MS+ K  NFLSQFRDQ TKKL+NFTAT+FAEVW+HYD+DGNG+IEG ELD FL EF+SSVVS               +   ++++SETAM  +K + MEA+DEN DG+IEI EL+QILP EENFLLLFRR+N ++SSV+F+++W+ FDK+ SGFIEA+ELK+FLK +L     + D P  ++K+ EYT  +LQLFD N+DGKLQLSEMARLLPV+EN+LCRPIFKNAS+I+  +I+++F +YD+D +GTIENEEL GFLKDL+E+ QE+       YDD D+QF K+ +L+QW+ + D KI+KD+L++IL QQ+R+
Sbjct:    1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVS---------------EDAGNEVVSETAMAQMKRDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSIL----DSADKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGMTQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRI 297
[Close]
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267FNU4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g3 PE=4 SV=1)

HSP 1 Score: 336.65 bits (862), Expect = 3.946e-111
Identity = 186/316 (58.86%), Postives = 247/316 (78.16%), Query Frame = 3 
Query:  108 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTP--KEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEE-------YDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            MS+ K  NFLSQFRDQ TKKL+NFTAT+FAEVW+HYD+DGNG+IEG ELD FL EF+SSVVS               +   ++++SETAM  +K + MEA+DEN DG+IEI EL+QILP EENFLLLFRR+N ++SSV+F+++W+ FDK+ SGFIEA+ELK+FLK +L     + D P  ++K+ EYT  +LQLFD N+DGKLQLSEMARLLPV+EN+LCRPIFKNAS+I+  +I+++F +YD+D +GTIENEEL GFLKDL+E+ QE+       YDD D+QF K+ +L+QW+ + D KI+KD+L++IL QQ+R+
Sbjct:    1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVS---------------EDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSIL----DSADKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGITQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRI 297
[Close]
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GV22 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g1 PE=4 SV=1)

HSP 1 Score: 336.65 bits (862), Expect = 4.078e-111
Identity = 186/316 (58.86%), Postives = 247/316 (78.16%), Query Frame = 3 
Query:  108 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTP--KEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEE-------YDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            MS+ K  NFLSQFRDQ TKKL+NFTAT+FAEVW+HYD+DGNG+IEG ELD FL EF+SSVVS               +   ++++SETAM  +K + MEA+DEN DG+IEI EL+QILP EENFLLLFRR+N ++SSV+F+++W+ FDK+ SGFIEA+ELK+FLK +L     + D P  ++K+ EYT  +LQLFD N+DGKLQLSEMARLLPV+EN+LCRPIFKNAS+I+  +I+++F +YD+D +GTIENEEL GFLKDL+E+ QE+       YDD D+QF K+ +L+QW+ + D KI+KD+L++IL QQ+R+
Sbjct:    1 MSHGKQKNFLSQFRDQGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVS---------------EDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSIL----DSADKPVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQEQGMTQKKDYDDQDMQFFKRVILDQWDVNHDGKISKDELKMILMQQSRI 297
[Close]
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A1I8GV54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003004g1 PE=4 SV=1)

HSP 1 Score: 326.635 bits (836), Expect = 4.667e-107
Identity = 177/304 (58.22%), Postives = 242/304 (79.61%), Query Frame = 3 
Query:  117 NKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            N+  NFL+QFRDQ T++L+NFTAT+FAEVW+HYDKDGNG+IEG ELD FL+EF+SSVV+E+                  +++SETA+ A+K + M+A+DEN DG+IEI EL+QILP EENFLLLFRR+N ++SSV+F+++WR FDK+ SG+IEA+EL++FLKH+L  A       ++K+ EYT  +LQLFD N+DGKLQLSEMA+LLPV+EN+LCRPIFKNA+RI   +I+++F +YD+D +G IENEEL GFLKDL+E+ QE+YD  D+ F KK +L+QW+ + D KI+KD+L++IL QQ+R+
Sbjct:   11 NQRANFLAQFRDQNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDI---------------GPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCADRC--VSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQEDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMILLQQSRL 297
[Close]
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267G6Z8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033745g2 PE=4 SV=1)

HSP 1 Score: 326.25 bits (835), Expect = 4.982e-107
Identity = 177/304 (58.22%), Postives = 242/304 (79.61%), Query Frame = 3 
Query:  117 NKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            N+  NFL+QFRDQ T++L+NFTAT+FAEVW+HYDKDGNG+IEG ELD FL+EF+SSVV+E+                  +++SETA+ A+K + M+A+DEN DG+IEI EL+QILP EENFLLLFRR+N ++SSV+F+++WR FDK+ SG+IEA+EL++FLKH+L  A       ++K+ EYT  +LQLFD N+DGKLQLSEMA+LLPV+EN+LCRPIFKNA+RI   +I+++F +YD+D +G IENEEL GFLKDL+E+ QE+YD  D+ F KK +L+QW+ + D KI+KD+L++IL QQ+R+
Sbjct:   11 NQRANFLAQFRDQNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDI---------------GPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCADRC--VSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQEDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMILLQQSRL 297
[Close]
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:103030695])

HSP 1 Score: 174.096 bits (440), Expect = 1.733e-51
Identity = 103/283 (36.40%), Postives = 163/283 (57.60%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELA-QILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            L N  A  F ++W H+D D NGYIEG ELD F +  ++ +                  G++   ++E  ++ LK   M AYD   DGR++I ELA  ILP +ENFLL+FRR+  L++SV+F+K+WR +D + SG+I A  ++             ++    K++EYT  ++ +FD NKDG+L L+++AR+L ++ENFL +      + + R  + EK+F  YD   +G +E  E+DGF+KD+ME+V+      +L   +K LL   + + D KI K++L L L
Sbjct:    5 LDNLDAAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKLR----------------PGDN---VTEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLLVFRREAPLDNSVEFMKIWRKYDADSSGYISA--VELKSFLQDLFQHHKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQFKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRPSISGSELDQFRKVLLRHCDINKDGKIQKNELALCL 266

HSP 2 Score: 88.5817 bits (218), Expect = 1.286e-19
Identity = 67/220 (30.45%), Postives = 120/220 (54.55%), Query Frame = 3 
Query:   93 VCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQ--LESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVR 746
            + N+++   +D NFL  FR +    L N  + EF ++W  YD D +GYI   EL  FL++                           K LS + ++   + +M  +D+N DGR+++ +LA+IL ++ENFLL F+ D    LE   DF K++  +D +++G +E  E+  F+K +++  +P+      ++D++ K++L+  D NKDGK+Q +E+A  L ++
Sbjct:   79 LANMILP--EDENFLLVFRREAP--LDN--SVEFMKIWRKYDADSSGYISAVELKSFLQDLFQ---------------------HHKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQFKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRPS--ISGSELDQFRKVLLRHCDINKDGKIQKNELALCLGMK 269

HSP 3 Score: 78.9518 bits (193), Expect = 2.813e-16
Identity = 53/170 (31.18%), Postives = 96/170 (56.47%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL-LPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            +  F+++W+ FD + +G+IE  EL  F +H++ + +P ++  +EK+ +     +  +D   DG+LQ+ E+A + LP  ENFL   +F+  + +    E  K++  YD D SG I   EL  FL+DL +  ++E     L+    T++N ++K+ D ++  +DL  IL  Q
Sbjct:   10 AAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKLRPGDNVTEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLL--VFRREAPLDNSVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKKELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQ 177
[Close]
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2a (calretinin [Source:NCBI gene;Acc:103033342])

HSP 1 Score: 173.326 bits (438), Expect = 2.749e-51
Identity = 118/284 (41.55%), Postives = 173/284 (60.92%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            L   TA++F ++W H+D DGNGYIEG EL+ F +E   +                    + + +     MK    + M+ +D+N DGRIE+ ELAQILP EENFLL FR+   + SS +F+  WR +D ++SG+IEANELK FL  LLK+A    D   +K+ EYT+ IL++FD N DGKL LSEMARLLPV+ENFL +  F+   ++T  +   +F  YD+D +G I+ +ELD  L+DL E  ++E D  DL   KK+++     S   K+ + +L+++L ++
Sbjct:   13 LAELTASQFLDIWKHFDTDGNGYIEGKELENFFRELEVARRGAGV--------------DPTNVSFREKMK----DFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSSEFMAAWRRYDTDRSGYIEANELKGFLSDLLKKANRHYDD--KKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGI-KLTAEQFNAIFTYYDKDGNGYIDEQELDALLRDLYEKNKKEVDTKDLTGYKKSIMAL---SDGGKLYRSELEIVLCRE 268

HSP 2 Score: 58.9214 bits (141), Expect = 1.736e-9
Identity = 60/212 (28.30%), Postives = 102/212 (48.11%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCR 764
            NFL  FR          +++EF   W  YD D +GYIE +EL  FL + +                      ++++   +  ++     I++ +D N DG++ + E+A++LP++ENFLL F+      ++  F  ++  +DK+ +G+I+  EL   L+ L ++ K   DT  + +  Y K I+ L D    GKL  SE+       E  LCR
Sbjct:   97 NFLLCFRQFVG------SSSEFMAAWRRYDTDRSGYIEANELKGFLSDLLK---------------------KANRHYDDKKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQGIKL--TAEQFNAIFTYYDKDGNGYIDEQELDALLRDLYEKNKKEVDT--KDLTGYKKSIMALSD---GGKLYRSEL-------EIVLCR 267
[Close]
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108])

HSP 1 Score: 166.392 bits (420), Expect = 1.473e-48
Identity = 113/284 (39.79%), Postives = 175/284 (61.62%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            L   TA++F +VW H+D DGNG+IEG E                  E+  E  +S  +G +++ ++  +++   ++ M+ +D+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F+  WR +D ++SGFIEANELK FL  LL +A    D   +K++EYT  IL++FD N DGKL LSEMARLLPV+ENFL +     + +++  E   +F+ YD+D +G I+  ELD  LKDL +    ++D   L   KK+++     S   K+ + +L+++L ++
Sbjct:   10 LAELTASQFMDVWRHFDSDGNGFIEGQE-----------------LEDFLEELESARKGVNAEPVN-ASLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKANRQYD--DKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK---FQSFKLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKHNMDFDSQTLASSKKSIMAL---SDGGKLFRTELEIVLCKE 265

HSP 2 Score: 57.7658 bits (138), Expect = 4.137e-9
Identity = 40/149 (26.85%), Postives = 75/149 (50.34%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIE 575
            NFL  FR          ++ EF   W  YD D +G+IE +EL  FL + ++                     ++++   +  +    + I++ +D N DG++ + E+A++LP++ENFLL F+      SS +F  ++  +DK+ +G+I+
Sbjct:   94 NFLLCFRQFVG------SSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLT---------------------KANRQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQSFKL--SSDEFSAIFMYYDKDGNGYID 213
[Close]
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108])

HSP 1 Score: 164.851 bits (416), Expect = 6.820e-48
Identity = 112/284 (39.44%), Postives = 168/284 (59.15%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            L   TA++F +VW H+D DGNG+IEG E                   E        ++   + + +   MK   ++ M+ +D+N+DG+IE+ ELAQILP EENFLL FR+   + SS +F+  WR +D ++SGFIEANELK FL  LL +A    D   +K++EYT  IL++FD N DGKL LSEMARLLPV+ENFL +     + +++  E   +F+ YD+D +G I+  ELD  LKDL +    ++D   L   KK+++     S   K+ + +L+++L ++
Sbjct:   16 LAELTASQFMDVWRHFDSDGNGFIEGQE------------------LEDFLEELESARKGVNPVNASLRMKI--SDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKANRQYD--DKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK---FQSFKLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKHNMDFDSQTLASSKKSIMAL---SDGGKLFRTELEIVLCKE 269

HSP 2 Score: 57.7658 bits (138), Expect = 4.237e-9
Identity = 40/149 (26.85%), Postives = 75/149 (50.34%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIE 575
            NFL  FR          ++ EF   W  YD D +G+IE +EL  FL + ++                     ++++   +  +    + I++ +D N DG++ + E+A++LP++ENFLL F+      SS +F  ++  +DK+ +G+I+
Sbjct:   98 NFLLCFRQFVG------SSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLT---------------------KANRQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQSFKL--SSDEFSAIFMYYDKDGNGYID 217
[Close]
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb1 (calbindin 1 [Source:NCBI gene;Acc:103033937])

HSP 1 Score: 156.377 bits (394), Expect = 6.083e-45
Identity = 114/281 (40.57%), Postives = 167/281 (59.43%), Query Frame = 3 
Query:  180 TATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSET-AMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            +A++F ++++HYD DGNGYIEG EL  F+KE                      Q      L  T  M+A     +E Y++N DG+I+I EL QILP EENFLL FR+  QL+S  +F++ WR +D + SG+IE++ELK FL+ LL+ AK   D  ++K++EYT   L++FD N DGKL LSEMARLLP  ENFL +       ++ R E  K+F +YD+D +G ++  ELD  LKDL E  ++  +   +Q  KK ++     S   K+ + +L L+L ++
Sbjct:   12 SASQFLDIFHHYDNDGNGYIEGKELQNFIKELQ--------------------QARKQAGLELTDQMRAF----VEEYEKNADGKIDIVELVQILPTEENFLLFFRQ--QLKSCTEFMEAWRTYDADHSGYIESDELKNFLRDLLQRAKKPFD--EKKLEEYTLTTLKIFDSNNDGKLCLSEMARLLPDDENFLLKF---EGVKMARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNKKVLEIGKIQVYKKAIMAL---SDGGKLYRTELALVLCEE 258

HSP 2 Score: 72.0182 bits (175), Expect = 5.670e-14
Identity = 68/222 (30.63%), Postives = 110/222 (49.55%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLL 737
            N+  +I +  +V     + NFL  FR Q        + TEF E W  YD D +GYIE DEL  FL++ +                       + K   E  ++      ++ +D NNDG++ + E+A++LP +ENFLL F       +  +F K++ ++DK+++G+++ NEL   LK L ++ K   +    KI  Y K I+ L D    GKL  +E+A +L
Sbjct:   68 NADGKIDIVELVQILPTEENFLLFFRQQLK------SCTEFMEAWRTYDADHSGYIESDELKNFLRDLLQ---------------------RAKKPFDEKKLEEYTLTTLKIFDSNNDGKLCLSEMARLLPDDENFLLKFEGVKM--ARKEFNKIFELYDKDRNGYMDENELDALLKDLCEKNKKVLEIG--KIQVYKKAIMALSD---GGKLYRTELALVL 255
[Close]
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010])

HSP 1 Score: 163.31 bits (412), Expect = 4.105e-48
Identity = 106/273 (38.83%), Postives = 166/273 (60.81%), Query Frame = 3 
Query:  198 EVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPME-ENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            EVW  +D D NGYIEG ELD F K  +                    Q  + + +S+  ++ ++  +M  YDE+ DGR+ + E+A +L  E E+FLL+F+R + L++SV+FIK+W  +D + SGFI A+ELK FLK LL          ++K++EYT  +++LFD NKDG+L L++MAR+L ++EN+L +   ++AS+   R + EK+F  YD   +G +E  E+DGF+KD+ME+V+      DL   + +LL + + + D KI K +L L L
Sbjct:   16 EVWQKFDADDNGYIEGKELDEFFKHLLK-------------------QLAAKECVSDEKVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLLIFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLLSH--HGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVADLSKFRDSLLRRCDTNQDGKIQKWELALCL 267

HSP 2 Score: 72.0182 bits (175), Expect = 1.480e-14
Identity = 61/176 (34.66%), Postives = 101/176 (57.39%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVR-ENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT-QQNRMYQ 1034
            S   ++VW+ FD + +G+IE  EL EF KHLLK+    E    EK++   + ++ ++D + DG+L LSE+A +L    E+FL   IFK AS +    E  K++  YD D SG I   EL  FLKDL+     + ++  L+    T++  ++K+ D ++  +D+  IL+ ++N + Q
Sbjct:   11 SQGLLEVWQKFDADDNGYIEGKELDEFFKHLLKQLAAKECVSDEKVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLL--IFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLLSHHGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQ 184

HSP 3 Score: 70.0922 bits (170), Expect = 6.196e-14
Identity = 59/220 (26.82%), Postives = 119/220 (54.09%), Query Frame = 3 
Query:   93 VCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVR 746
            V N++++  +D +FL  F+  ++  L N  + EF ++W+ YD D +G+I   EL  FLK+ +                         + + E  ++   + +M+ +D+N DGR+++ ++A+IL ++EN+LL F  +  +Q E   DF K++  +D +++G +E  E+  F+K +++  +P+       + ++   +L+  D N+DGK+Q  E+A  L +R
Sbjct:   80 VANMLLA--EDESFLLIFKRASS--LDN--SVEFIKIWHQYDLDSSGFISASELKNFLKDLL---------------------SHHGRQVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVA--DLSKFRDSLLRRCDTNQDGKIQKWELALCLGIR 270
[Close]
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435])

HSP 1 Score: 85.8853 bits (211), Expect = 1.464e-20
Identity = 49/123 (39.84%), Postives = 81/123 (65.85%), Query Frame = 3 
Query:  645 EKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
            +K++EYT+  L++FD N+DGKL+LSEMARLLPV+ENFL +  F+  + +T  E   +F  YD+D +G I+ +EL+  L+DL E  +++ D   L   K+ ++     S   K+ ++DL+++L 
Sbjct:   19 KKLNEYTQTTLKMFDSNRDGKLELSEMARLLPVQENFLLK--FQGVT-MTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNRKDLDAQTLTSYKRNIMAL---SDHGKLYRNDLEMVLC 135

HSP 2 Score: 47.3654 bits (111), Expect = 5.860e-7
Identity = 34/115 (29.57%), Postives = 73/115 (63.48%), Query Frame = 3 
Query:  399 MEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPV 743
            ++ +D N DG++E+ E+A++LP++ENFLL F+      ++ +F  ++  +DK+++G+I+ +EL+  L+ L ++ +  +D   + +  Y + I+ L D+   GKL  +++  +L V
Sbjct:   29 LKMFDSNRDGKLELSEMARLLPVQENFLLKFQ--GVTMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNR--KDLDAQTLTSYKRNIMALSDH---GKLYRNDLEMVLCV 136
[Close]
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000059.1 (pep scaffold:Pmarinus_7.0:GL485405:3181:11335:1 gene:ENSPMAG00000000055.1 transcript:ENSPMAT00000000059.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.0333 bits (201), Expect = 6.715e-20
Identity = 39/74 (52.70%), Postives = 54/74 (72.97%), Query Frame = 3 
Query:  360 LSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEAN 581
            L E+ +K      ME YD+N DG+IE+ ELAQILP EENFLL FR+   ++SS +F++ WR +D ++SG+IEAN
Sbjct:    3 LPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFLLFFRQ--HVKSSAEFMETWRRYDTDRSGYIEAN 74

HSP 2 Score: 48.521 bits (114), Expect = 4.636e-8
Identity = 27/79 (34.18%), Postives = 45/79 (56.96%), Query Frame = 3 
Query:  630 EDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEEL 866
            ++ P+  + E  K  ++ +D N DGK++++E+A++LP  ENFL   +F      +  E  + +  YD D SG IE  EL
Sbjct:    1 QELPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFL---LFFRQHVKSSAEFMETWRRYDTDRSGYIEANEL 76
[Close]
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001806.1 (pep scaffold:Pmarinus_7.0:GL476330:305753:307416:1 gene:ENSPMAG00000001642.1 transcript:ENSPMAT00000001806.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.5362 bits (140), Expect = 7.725e-12
Identity = 28/47 (59.57%), Postives = 38/47 (80.85%), Query Frame = 3 
Query:  393 EIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIK 533
            E ME +D+N+DGRIE+ ELAQILP EENFLL FR+   ++SS +F++
Sbjct:    8 EFMEKFDQNSDGRIELAELAQILPTEENFLLFFRQ--HVKSSAEFME 52
[Close]
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45967 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ7])

HSP 1 Score: 130.183 bits (326), Expect = 2.546e-35
Identity = 95/285 (33.33%), Postives = 159/285 (55.79%), Query Frame = 3 
Query:  165 KLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQ--EEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
            K    +  EF +V+  +D D NGYIE  E+DLF+ + +  +                   E +K  +E A K  K EI+E YD + DGR  + EL  ILP+E N L  +  + +   SVDF+K++  FD+++SGF+E  EL+ F   LL     +   P+ K+D+Y +  L+ +D NKDGK  L+E+A++LP+ EN      +     +TR +  K++  YD+D+SG IE +E+   L D+++  Q   EY+  DL+  +  +L  ++++ D K+++D+L  +L+
Sbjct:    4 KATKLSPVEFVKVFMKFDSDKNGYIEEKEMDLFIHDLLKELN----------------DKEPTK--AEVADK--KKEILEKYDLDKDGRFSMDELENILPVEPNLLRTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLSMKGKSSINPR-KLDDYIEYCLEKYDANKDGKFNLNELAKMLPLEENIF--ETYGIKRDMTREDFNKVWDYYDKDNSGEIEGDEILALLHDVLKQQQPDREYNKPDLERFRDIILEIYDENKDNKLSRDELCCLLS 265

HSP 2 Score: 67.781 bits (164), Expect = 6.452e-13
Identity = 54/176 (30.68%), Postives = 94/176 (53.41%), Query Frame = 3 
Query:  513 SSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIV-QEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRMYQS 1037
            S V+F+KV+  FD +++G+IE  E+  F+  LLKE    E T  E  D+  K IL+ +D +KDG+  + E+  +LPV  N L R       RI   +  K+F  +D+D SG +E  EL GF  +L+ +  +   +   L    +  L +++ + D K   ++L  +L  +  ++++
Sbjct:    9 SPVEFVKVFMKFDSDKNGYIEEKEMDLFIHDLLKELNDKEPTKAEVADK-KKEILEKYDLDKDGRFSMDELENILPVEPNLL-RTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLSMKGKSSINPRKLDDYIEYCLEKYDANKDGKFNLNELAKMLPLEENIFET 182
[Close]
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45965 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ5])

HSP 1 Score: 107.842 bits (268), Expect = 7.978e-28
Identity = 84/207 (40.58%), Postives = 119/207 (57.49%), Query Frame = 3 
Query:  135 LSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENF 755
            L +F           T+ +F +++  YD DGN  I+  ELDLFL                        Q       + T + ALKNEI+E YD N DG I++ ELAQ LP+EENFL+ FR+DN + SSVDF+K+++ +D ++SG+IE  ELK FL  LL   K        +I++Y   ++++FD NKDGKL+LSE++RLL V +NF
Sbjct:    8 LLKFVKSNYAGCAKLTSEQFYDIFKKYDADGNDCIDAKELDLFL------------------------QDVQKADPNATNLDALKNEILEKYDANYDGVIQMSELAQCLPLEENFLVGFRKDNVI-SSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLDADKKA--ISPSRINDYASAMIEIFDRNKDGKLELSELSRLLQVEDNF 187

HSP 2 Score: 59.3066 bits (142), Expect = 1.752e-10
Identity = 33/74 (44.59%), Postives = 47/74 (63.51%), Query Frame = 3 
Query:  672 ILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLME 893
            IL+ +D N DG +Q+SE+A+ LP+ ENFL    F+  + I+  +  K+F  YD D SG IE  EL  FL DL++
Sbjct:   71 ILEKYDANYDGVIQMSELAQCLPLEENFLVG--FRKDNVISSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLD 142
[Close]
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45966 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ6])

HSP 1 Score: 100.138 bits (248), Expect = 3.172e-24
Identity = 79/272 (29.04%), Postives = 138/272 (50.74%), Query Frame = 3 
Query:  198 EVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQ-FLKKTLLNQWNKSADAKITKDDLQLIL 1010
            E+++ +DKDGNG+I+  ELD F+K+                          +  LSE  + + K  ++   DENND +I   EL ++LP + NF+  F    +    VDFIK+W+ +DK+ SGF+E       L+  L+E      TP ++IDE  +   Q      DGK  + + + +  +     C  +  NA    + + ++ F  YD+D+SG IE+ EL+   +DL++I   +  + D++ +++  ++   +K+ D KI KD+L+  L
Sbjct:   23 EIFSKFDKDGNGFIDAKELDSFIKDI------------------------CNNELSEDDVASFKTGLLAKLDENNDNKISKKELRKVLPTKSNFMRQFLTTKEF-CQVDFIKIWKNYDKDGSGFLEK----PELQLFLEELFAVNKTPGQRIDEKEREKFQTILL--DGKEGVDKKSLMKHLGVKAACDEVAANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLKINDADITEEDVKDYVQSVVMPDLDKNKDNKIQKDELKSYL 263
[Close]
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO46016 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ4])

HSP 1 Score: 52.373 bits (124), Expect = 1.678e-7
Identity = 35/133 (26.32%), Postives = 65/133 (48.87%), Query Frame = 3 
Query:  651 IDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDH-DLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRMYQSELS 1046
            I    K  L   D ++ G++ L EMAR L V +NFL         ++ + +  +++  YDQD  G+I+  ELD  ++D+   +Q    +  D++  K  ++   +   D  I+K++L +   Q  +  +  LS
Sbjct:  205 IGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNINPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDVFAKIQGRSPEMDDIEKWKDIIMMFADADGDGAISKEELNIFFAQGLKEAKKRLS 337
[Close]
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000016338.2 (calretinin [Source:NCBI gene;Acc:101174993])

HSP 1 Score: 171.014 bits (432), Expect = 2.129e-50
Identity = 114/284 (40.14%), Postives = 172/284 (60.56%), Query Frame = 3 
Query:  168 LQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQ 1019
            L   TA++F ++W H+D DGNGYIEG EL+ F +E  +S                          S    +    E M+ +D+N DGRIE+ ELAQILP EENFLL FR+   + SS +F+  WR +D ++SG+IEANELK FL  LL++A    D  ++K+ EYT+ IL++FD N DGKL LSEMARLLPV+ENFL +  F+   ++T  +   +F  YD+D +G I+ +EL+  L+DL +  ++E D   L   K+++++    S   K+ + +L+++L ++
Sbjct:   13 LAEVTASQFLDIWKHFDVDGNGYIEGKELENFFRELETSRRGAGVDP------------------SNPQFREKMREFMQKFDKNRDGRIEMSELAQILPTEENFLLCFRQ--FVSSSAEFMAAWRRYDTDRSGYIEANELKGFLSDLLEKANRHYD--EQKLHEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGV-KLTSEQFNSIFTYYDKDGNGYIDEQELEALLRDLYQTNKKEIDAKSLMSYKESIMSL---SDGGKLYRGELEIVLCKE 268

HSP 2 Score: 59.3066 bits (142), Expect = 1.272e-9
Identity = 41/149 (27.52%), Postives = 72/149 (48.32%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIE 575
            NFL  FR   +      ++ EF   W  YD D +GYIE +EL  FL + +                      ++++   E  +      I+  +D N DG++ + E+A++LP++ENFLL F+      +S  F  ++  +DK+ +G+I+
Sbjct:   97 NFLLCFRQFVS------SSAEFMAAWRRYDTDRSGYIEANELKGFLSDLLE---------------------KANRHYDEQKLHEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQGVKL--TSEQFNSIFTYYDKDGNGYID 216
[Close]
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606])

HSP 1 Score: 171.014 bits (432), Expect = 2.691e-50
Identity = 117/288 (40.62%), Postives = 170/288 (59.03%), Query Frame = 3 
Query:  165 KLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            +L   TA +F ++W H+D DGNGYIEG EL+ F  +  S+                          S  + K    E M  +D+N+DGRIE+ ELAQILP EENFLL FR    + SS +F+  WR +D ++SG+IE+NELK FL  LLK+A    D   +++ EYT+ IL++FD N DGKL LSEMARLLPV+ENFL +  F+   R+T  E + LF  YD+D +G I+ +ELD  LKDL +  + + D   L   KK+++     S   K+ + +++++L + + +
Sbjct:   12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP------------------SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKANRIYD--DKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQGI-RLTVKEFDSLFTFYDKDGNGYIDEQELDALLKDLCDKNKMDVDATGLVDYKKSIMAL---SDGGKLYRTEMEIVLCRDSML 271

HSP 2 Score: 59.6918 bits (143), Expect = 9.735e-10
Identity = 66/232 (28.45%), Postives = 108/232 (46.55%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSV-DFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCR 764
            NS  RI +  +      + NFL  FR+         ++ EF   W  YD D +GYIE +EL  FL + +                      ++++I  +  +      I+  +D N DG++ + E+A++LP++ENFLL F+    +  +V +F  ++  +DK+ +G+I+  EL   LK L  + K   D     + +Y K I+ L D    GKL  +EM       E  LCR
Sbjct:   78 NSDGRIEMSELAQILPTEENFLLCFREFVG------SSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLK---------------------KANRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQ---GIRLTVKEFDSLFTFYDKDGNGYIDEQELDALLKDLCDKNKM--DVDATGLVDYKKSIMALSD---GGKLYRTEM-------EIVLCR 267
[Close]
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000006100.2 (secretagogin [Source:NCBI gene;Acc:101157482])

HSP 1 Score: 169.474 bits (428), Expect = 1.031e-49
Identity = 110/281 (39.15%), Postives = 165/281 (58.72%), Query Frame = 3 
Query:  174 NFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQ-ILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            N  A  F E+W H+D D NGYIEG ELD F    +  +                      +  +E  ++ LK   M AYD   DGR++I ELA  ILP EENFLL+F R+  L++SVDF+K+WR +D + SG+I A ELK FLK L ++     +   +K++EYT  ++++FD NKDG+L L+++AR+L + +NFL +     +S+  R  + EK+F  YD   +G +E  E+DGF+KD+ME+V+      +L  L+  LL+  + + D KI K++L L L
Sbjct:    7 NLDAAGFLEIWQHFDADDNGYIEGKELDEFFHHMMQRLG-------------------PQEETTEERVRELKERFMSAYDVTADGRLQIQELASMILPEEENFLLIFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHH--REVSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMDASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELVRPSLSGPELDKLRAVLLHHCDVNNDGKIQKNELALCL 266

HSP 2 Score: 77.7962 bits (190), Expect = 7.795e-16
Identity = 54/191 (28.27%), Postives = 103/191 (53.93%), Query Frame = 3 
Query:  180 TATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRD--NQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVR 746
             + +F ++W  YD D +GYI   EL  FLK+                        +  + +S   ++   + +M+ +D+N DGR+++ +LA++L +E+NFLL F  D  ++ E   DF K++  +D +++G +E  E+  F+K +++  +P+   P  ++D+   ++L   D N DGK+Q +E+A  L V+
Sbjct:  102 NSVDFMKIWRKYDADCSGYISAQELKAFLKDLFE---------------------QHHREVSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMDASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELVRPSLSGP--ELDKLRAVLLHHCDVNNDGKIQKNELALCLGVK 269

HSP 3 Score: 66.2402 bits (160), Expect = 6.469e-12
Identity = 55/168 (32.74%), Postives = 92/168 (54.76%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL-LPVRENFLCRPIF-KNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
            +  F+++W+ FD + +G+IE  EL EF  H+++   P E+T +E++ E  +  +  +D   DG+LQ+ E+A + LP  ENFL   IF + A      +  K++  YD D SG I  +EL  FLKDL E    E     L+     ++  ++K+ D ++  +DL  +L 
Sbjct:   10 AAGFLEIWQHFDADDNGYIEGKELDEFFHHMMQRLGPQEETTEERVRELKERFMSAYDVTADGRLQIQELASMILPEEENFLL--IFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHHREVSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLA 175
[Close]
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606])

HSP 1 Score: 160.614 bits (405), Expect = 2.362e-46
Identity = 115/288 (39.93%), Postives = 167/288 (57.99%), Query Frame = 3 
Query:  165 KLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            +L   TA +F ++W H+D DGNGYIEG EL+ F  +  S+                          S  + K    E M  +D+N+DGRIE+ ELAQILP EENFLL FR    + SS +F+  WR +D ++SG+IE+NELK FL  LLK+A    D   +++ EYT+ IL++FD N DGKL LSEMARLLPV+ENFL +  F+    I+   +    LVY QD +G I+ +ELD  LKDL +  + + D   L   KK+++     S   K+ + +++++L + + +
Sbjct:   12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP------------------SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKANRIYD--DKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK--FQKRPPISLTCV----LVY-QDGNGYIDEQELDALLKDLCDKNKMDVDATGLVDYKKSIMAL---SDGGKLYRTEMEIVLCRDSML 267

HSP 2 Score: 56.6102 bits (135), Expect = 9.000e-9
Identity = 39/142 (27.46%), Postives = 67/142 (47.18%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRR 497
            NS  RI +  +      + NFL  FR+         ++ EF   W  YD D +GYIE +EL  FL + +                      ++++I  +  +      I+  +D N DG++ + E+A++LP++ENFLL F++
Sbjct:   78 NSDGRIEMSELAQILPTEENFLLCFREFVG------SSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLK---------------------KANRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQK 192
[Close]
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000041433.1 (secretagogin [Source:NCBI gene;Acc:101157482])

HSP 1 Score: 140.584 bits (353), Expect = 4.842e-39
Identity = 94/272 (34.56%), Postives = 150/272 (55.15%), Query Frame = 3 
Query:  174 NFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEA--YDENNDGRIEIYELAQ-ILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITR-YEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADA 977
            N  A  F E+W H+D D NGYIEG ELD F                                     M+ L  ++  A  +  +    + +++LA  ILP EENFLL+F R+  L++SVDF+K+WR +D + SG+I A ELK FLK L ++     +   +K++EYT  ++++FD NKDG+L L+++AR+L + +NFL +     +S+  R  + EK+F  YD   +G +E  E+DGF+KD+ME+V+      +L  L+  LL+  +  +++
Sbjct:    7 NLDAAGFLEIWQHFDADDNGYIEGKELDEFF---------------------------------HHMMQRLGPQVRVANPFFPHTSALLPVFQLASMILPEEENFLLIFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHH--REVSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMDASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELVRPSLSGPELDKLRAVLLHHCDPQSNS 243

HSP 2 Score: 52.7582 bits (125), Expect = 1.809e-7
Identity = 50/167 (29.94%), Postives = 81/167 (48.50%), Query Frame = 3 
Query:  516 SVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIF-KNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILT 1013
            +  F+++W+ FD + +G+IE  EL EF  H+++   P           +T  +L +F        QL+ M  +LP  ENFL   IF + A      +  K++  YD D SG I  +EL  FLKDL E    E     L+     ++  ++K+ D ++  +DL  +L 
Sbjct:   10 AAGFLEIWQHFDADDNGYIEGKELDEFFHHMMQRLGPQVRVANPFF-PHTSALLPVF--------QLASM--ILPEEENFLL--IFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHHREVSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLA 163
[Close]
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1)

HSP 1 Score: 336.265 bits (861), Expect = 7.204e-116
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 3 
Query:  108 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 734
            MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL
Sbjct:    1 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 209

HSP 2 Score: 56.225 bits (134), Expect = 6.053e-9
Identity = 47/163 (28.83%), Postives = 85/163 (52.15%), Query Frame = 3 
Query:  474 NFLLLFR----RDNQLESSVDFIKVWRMFDKNQSGFIEANXX-------XXXXXXXXXXXXPTEDTPKEKIDEYTKI------------ILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLM 890
            NFL  FR    +  Q  ++ +F +VW  +DK+ +G+IE +EL          +       +  E    E   E +KI            I++ +D N DG++++ E+A++LP+ ENFL   +F+  +++ +  +  K++ ++D++ SG IE  EL  FLK L+
Sbjct:    8 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLL 168
[Close]
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1)

HSP 1 Score: 320.472 bits (820), Expect = 1.016e-109
Identity = 193/209 (92.34%), Postives = 194/209 (92.82%), Query Frame = 3 
Query:  108 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 734
            MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFS           +    KILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL
Sbjct:    1 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFS-----------EESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 198

HSP 2 Score: 60.077 bits (144), Expect = 2.104e-10
Identity = 50/152 (32.89%), Postives = 88/152 (57.89%), Query Frame = 3 
Query:  474 NFLLLFR----RDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPT--EDTPKEKIDEYTKI------ILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLM 890
            NFL  FR    +  Q  ++ +F +VW  +DK+ +G+IE +EL  FLK  +         +  KEKI   T +      I++ +D N DG++++ E+A++LP+ ENFL   +F+  +++ +  +  K++ ++D++ SG IE  EL  FLK L+
Sbjct:    8 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLL 157
[Close]
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000055605.1 (SMESG000055605.1)

HSP 1 Score: 315.849 bits (808), Expect = 1.999e-106
Identity = 173/309 (55.99%), Postives = 234/309 (75.73%), Query Frame = 3 
Query:  108 MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGE--SSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            MS  K  NFL+Q++D  TKKL+NFTAT+FAEVW+HYD DGNGYIEG ELD FL EF+ S++                 GE  +   L++  +  LK + ++A+D+N+D +IE+ ELAQ+LP +E FLLLFR+D  L+SSV+F+K+W+ FDK+QSGFIEA+ELK FL   LKEA+P E   +EK+ EYT  +LQLFD NKDGKLQLSEMARLLPV+EN+LCRPIFKNA +IT  +IE +F +YD+D +GTIENEEL GFLKDL+ +VQE+YD  DL+F ++ +LN+W+ + D KI+ ++L+++L QQ+R+
Sbjct:    1 MSTTKFTNFLAQYKDNETKKLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSIL-----------------GEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLKEARPEEQILEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMMLMQQSRL 292

HSP 2 Score: 74.3294 bits (181), Expect = 1.284e-14
Identity = 63/224 (28.12%), Postives = 121/224 (54.02%), Query Frame = 3 
Query:   72 NSINRIIVCNIVMSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLL--LFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLL 737
            NS N+I +  +      D  FL  FR          ++ EF ++W  +DKD +G+IE DEL  FL                   ++   +    + + E  +    + +++ +D N DG++++ E+A++LP++EN+L   +F+  +++ ++VD   V+ ++D++++G IE  EL+ FLK LL   +  ED  K+ ++ + +IIL  +D N DGK+ ++E+  +L
Sbjct:   89 NSDNKIEMSELAQVLPTDEVFLLLFRKDAPLD----SSVEFMKIWKQFDKDQSGFIEADELKNFL-------------------SEFLKEARPEEQILEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKI-TTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQ--EDYDKDDLEFFRQIILNEWDVNHDGKISMNELKMML 286
[Close]
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000023194.1 (SMESG000023194.1)

HSP 1 Score: 305.064 bits (780), Expect = 4.122e-102
Identity = 172/309 (55.66%), Postives = 228/309 (73.79%), Query Frame = 3 
Query:  129 NFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRMYQSELSLRK 1055
            NFLS+FRD  T++  NFTA +F EVW HYD DGNGYIEG+ELD FL+EF+SSVV  +                 S+I+S++A+  +K + M AYDEN D +IEI ELAQ+LP +ENFLLLFRRDN LESSVDF+K+WR FD + SG+IE++ELKEFL++L+ E+   E    EK++EY+  ILQLFD N DGKLQLSEMARLLPV+EN+L +P+FKN +RIT Y+I+++F  YD D +GTIENEEL G LKDL+E+  E YD+  ++F ++ +L QW+   D KI +D+L++IL QQ++M Q E   RK
Sbjct:   10 NFLSKFRDSKTRRWTNFTAKQFLEVWCHYDTDGNGYIEGEELDGFLREFISSVVPGSI---------------GSEIISDSALSDMKEDFMAAYDENEDNKIEIGELAQMLPTDENFLLLFRRDNPLESSVDFMKIWRKFDTDNSGYIESDELKEFLRYLISESNVKE-ISDEKLEEYSYTILQLFDENGDGKLQLSEMARLLPVKENYLSKPLFKNVNRITGYDIDRVFKKYDTDKNGTIENEELQGLLKDLLELAGESYDEEKMKFYQEVILGQWDLDHDGKIGRDELKMILLQQSKMAQEEEIARK 302
[Close]
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000003798.1 (SMESG000003798.1)

HSP 1 Score: 252.292 bits (643), Expect = 4.414e-81
Identity = 139/302 (46.03%), Postives = 207/302 (68.54%), Query Frame = 3 
Query:  129 NFLSQFRD-QTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLXXXXXXXXXXXXXXXXXXXXXXXXQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANXXXX-XXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLILTQQNRM 1028
            N+L+++R+ +   +L  FTAT+F +VWNH+D D NGYIE  ELD FL +F +SV+S               +    ++LS++A +A+K   +EAYDEN DGRIEI EL+QILP +E FL+LF+R+N + SSV+F+K+W+ FDK++SGFIEANEL+E   + L    K  E   + KI  +T I+L+LFD N DGKLQLSEMARLLP++EN+L +P+  N  +I+  E+E++F  YD D +G ++  E+   L DLME+ +E YDD  L+  +  +L+QW+ + D KI+  +L++ILTQ +++
Sbjct:   26 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVIS---------------EPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKSAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEPYDDKKLEKFQNVILSQWDVNKDGKISMKELKMILTQHSKL 312

HSP 2 Score: 62.7734 bits (151), Expect = 1.028e-10
Identity = 56/213 (26.29%), Postives = 111/213 (52.11%), Query Frame = 3 
Query:  426 GRIEIY-----ELAQILPMEENFLLLFRR---DNQLE--SSVDFIKVWRMFDKNQSGFIEA----NXXXXXXXXXXXXXXPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARLLPVRENFLCRPIFKNASRI-TRYEIEKLFLVYDQDHSGTIENEELDGFLKDLMEIVQ---EEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLIL 1010
            GRI ++     EL       +N+L  +R    +NQL   ++  F+ VW  FD + +G+IEA    N L +F   ++ E    E   +       +  L+ +D N+DG++++ E++++LP  E FL   +F+  + I +  E  K++  +D+D SG IE  EL  F+ +L++  Q   E+ ++  + +    +L  +++++D K+   ++  +L
Sbjct:    5 GRINLHSGLELELEDSQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLV--LFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKSAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLL 215
The following BLAST results are available for this feature:
BLAST of Calbindin-32 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CALB26.090e-5542.51calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
CALB26.090e-5542.51calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
CALB11.466e-4540.14calbindin 1 [Source:HGNC Symbol;Acc:HGNC:1434][more]
SCGN2.664e-4537.63secretagogin, EF-hand calcium binding protein [Sou... [more]
CALB27.377e-4542.86calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
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BLAST of Calbindin-32 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calbindin-32 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Cbp53E6.360e-9253.36gene:FBgn0004580 transcript:FBtr0340072[more]
Cbp53E6.360e-9253.36gene:FBgn0004580 transcript:FBtr0087056[more]
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BLAST of Calbindin-32 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scgn2.529e-5438.46secretagogin, EF-hand calcium binding protein [Sou... [more]
scgn2.529e-5438.46secretagogin, EF-hand calcium binding protein [Sou... [more]
calb2a3.931e-5442.40calbindin 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1677... [more]
calb2b3.505e-5041.34calbindin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1668... [more]
calb15.783e-4541.28calbindin 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8107][more]
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BLAST of Calbindin-32 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scgn3.007e-5036.33secretagogin, EF-hand calcium binding protein [Sou... [more]
scgn4.759e-4734.66secretagogin, EF-hand calcium binding protein [Sou... [more]
gata51.012e-4148.24GATA binding protein 5 [Source:Xenbase;Acc:XB-GENE... [more]
stim11.007e-3942.25stromal interaction molecule 1 [Source:Xenbase;Acc... [more]
stim17.475e-3837.95stromal interaction molecule 1 [Source:Xenbase;Acc... [more]
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BLAST of Calbindin-32 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Calb21.297e-5442.61calbindin 2 [Source:MGI Symbol;Acc:MGI:101914][more]
Calb23.636e-5247.11calbindin 2 [Source:MGI Symbol;Acc:MGI:101914][more]
Calb11.645e-4641.22calbindin 1 [Source:MGI Symbol;Acc:MGI:88248][more]
Scgn3.618e-4032.53secretagogin, EF-hand calcium binding protein [Sou... [more]
Scgn1.078e-1840.00secretagogin, EF-hand calcium binding protein [Sou... [more]
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BLAST of Calbindin-32 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P41044|CAB32_DROME6.375e-9153.36Calbindin-32 OS=Drosophila melanogaster OX=7227 GN... [more]
sp|P22676|CALB2_HUMAN2.925e-5442.51Calretinin OS=Homo sapiens OX=9606 GN=CALB2 PE=2 S... [more]
sp|P47728|CALB2_RAT3.396e-5442.51Calretinin OS=Rattus norvegicus OX=10116 GN=Calb2 ... [more]
sp|Q3ZBY3|CALB2_BOVIN8.510e-5443.51Calretinin OS=Bos taurus OX=9913 GN=CALB2 PE=2 SV=... [more]
sp|Q08331|CALB2_MOUSE9.073e-5442.61Calretinin OS=Mus musculus OX=10090 GN=Calb2 PE=1 ... [more]
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BLAST of Calbindin-32 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GD683.861e-11158.86Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FNU43.946e-11158.86Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267GV224.078e-11158.86Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8GV544.667e-10758.22Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267G6Z84.982e-10758.22Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Calbindin-32 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
scgn1.733e-5136.40secretagogin, EF-hand calcium binding protein [Sou... [more]
calb2a2.749e-5141.55calretinin [Source:NCBI gene;Acc:103033342][more]
calb2b1.473e-4839.79calretinin-like [Source:NCBI gene;Acc:103034108][more]
calb2b6.820e-4839.44calretinin-like [Source:NCBI gene;Acc:103034108][more]
calb16.083e-4540.57calbindin 1 [Source:NCBI gene;Acc:103033937][more]
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BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 4
Match NameE-valueIdentityDescription
scgn4.105e-4838.83secretagogin, EF-hand calcium binding protein [Sou... [more]
CALB21.464e-2039.84calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435][more]
ENSPMAT00000000059.16.715e-2052.70pep scaffold:Pmarinus_7.0:GL485405:3181:11335:1 ge... [more]
ENSPMAT00000001806.17.725e-1259.57pep scaffold:Pmarinus_7.0:GL476330:305753:307416:1... [more]
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BLAST of Calbindin-32 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calbindin-32 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO459672.546e-3533.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459657.978e-2840.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459663.172e-2429.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460161.678e-726.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calbindin-32 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000016338.22.129e-5040.14calretinin [Source:NCBI gene;Acc:101174993][more]
calb2b2.691e-5040.63calretinin [Source:NCBI gene;Acc:101167606][more]
ENSORLT00000006100.21.031e-4939.15secretagogin [Source:NCBI gene;Acc:101157482][more]
calb2b2.362e-4639.93calretinin [Source:NCBI gene;Acc:101167606][more]
ENSORLT00000041433.14.842e-3934.56secretagogin [Source:NCBI gene;Acc:101157482][more]
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BLAST of Calbindin-32 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043906.17.204e-116100.00SMESG000043906.1[more]
SMESG000043906.11.016e-10992.34SMESG000043906.1[more]
SMESG000055605.11.999e-10655.99SMESG000055605.1[more]
SMESG000023194.14.122e-10255.66SMESG000023194.1[more]
SMESG000003798.14.414e-8146.03SMESG000003798.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014166 ID=SMED30014166|Name=Calbindin-32|organism=Schmidtea mediterranea sexual|type=transcript|length=1169bp
TTTCAATACTGGTGAAATAATTCCAATATTGGCAAATAATCGATTTGCAT
GTGTATTGAGTAAATCTTATCAACTCAATCAATCGCATTATTGTTTGCAA
TATCGTCATGTCTAACAATAAAGATTTTAATTTTTTATCACAGTTTCGCG
ATCAAACAACCAAAAAATTGCAAAATTTCACAGCTACCGAATTCGCCGAA
GTCTGGAATCATTATGACAAAGATGGTAATGGGTACATTGAAGGTGACGA
GCTGGATTTATTTCTAAAAGAATTCGTTTCAAGTGTTGTATCAGAAAATT
TTTCTGAAGAGAGTAAAGAGTTAAACAAATCTGAAAGTCAGGGAGAATCT
TCAAAAATACTGTCAGAAACAGCTATGAAAGCCTTAAAAAACGAAATCAT
GGAGGCTTATGATGAGAACAATGATGGAAGAATTGAAATTTATGAGCTCG
CACAAATTTTACCGATGGAGGAGAATTTTCTTCTCCTATTTCGAAGAGAC
AATCAATTGGAATCAAGCGTGGACTTTATAAAGGTATGGAGAATGTTTGA
TAAAAACCAAAGCGGATTTATTGAAGCAAATGAACTCAAGGAATTTTTAA
AACATTTATTGAAAGAAGCTAAACCAACGGAAGACACTCCAAAAGAAAAG
ATTGATGAATACACAAAGATTATATTACAATTATTCGATTATAATAAAGA
TGGAAAACTGCAACTCAGTGAGATGGCAAGATTGTTACCAGTTAGGGAGA
ACTTTCTTTGCAGGCCTATTTTCAAAAATGCCAGCAGAATCACAAGATAT
GAAATCGAAAAACTTTTCTTGGTGTACGACCAGGATCACAGTGGGACGAT
TGAAAACGAAGAATTAGATGGGTTTCTTAAAGATTTAATGGAAATTGTCC
AAGAAGAATATGATGACCATGATTTACAATTTTTAAAAAAGACTCTTCTC
AATCAGTGGAACAAAAGCGCCGATGCAAAAATAACTAAAGATGATTTGCA
GCTAATTCTAACGCAACAAAATCGAATGTACCAATCAGAACTCAGTTTGC
GGAAAAAATGAAATTATCAATAATTTCCTCTTTTATTTAACTAGAAAATA
ACAGTTTTTTCCTATATTGTTCCTCGAATCGTATTTTTTATTTATAAAAT
CAAATTAAACCATGTTGTT
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protein sequence of SMED30014166-orf-1

>SMED30014166-orf-1 ID=SMED30014166-orf-1|Name=SMED30014166-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=318bp
MSNNKDFNFLSQFRDQTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELD
LFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEA
YDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKN
QSGFIEANELKEFLKHLLKEAKPTEDTPKEKIDEYTKIILQLFDYNKDGK
LQLSEMARLLPVRENFLCRPIFKNASRITRYEIEKLFLVYDQDHSGTIEN
EELDGFLKDLMEIVQEEYDDHDLQFLKKTLLNQWNKSADAKITKDDLQLI
LTQQNRMYQSELSLRKK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 90..110
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 110..209
e-value: 4.3E-25
score: 89.6
coord: 210..302
e-value: 4.3E-15
score: 57.5
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 13..107
e-value: 2.5E-18
score: 67.9
NoneNo IPR availablePANTHERPTHR19972CALBINDINcoord: 15..308
IPR002048EF-hand domainSMARTSM00054efh_1coord: 93..121
e-value: 2.0
score: 15.3
coord: 28..56
e-value: 5.7E-5
score: 32.5
coord: 185..213
e-value: 33.0
score: 5.6
coord: 232..260
e-value: 0.0011
score: 28.3
coord: 139..167
e-value: 3.0E-5
score: 33.4
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 233..254
e-value: 0.11
score: 12.0
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 142..208
e-value: 2.9E-9
score: 37.2
coord: 30..117
e-value: 2.9E-7
score: 30.8
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 89..124
score: 9.185
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 181..216
score: 7.874
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 135..170
score: 14.011
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 24..59
score: 12.617
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 232..263
score: 10.72
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 37..49
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 241..253
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 194..206
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 148..160
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 102..114
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 179..304
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 27..209