Nuclease

Overview
NameNuclease
Smed IDSMED30014095
Length (bp)1000
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Nuclease (SMED30014095) t-SNE clustered cells

Violin plots show distribution of expression levels for Nuclease (SMED30014095) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Nuclease (SMED30014095) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Nuclease (SMED30014095) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014095

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30014095SMESG000013712.1 dd_Smed_v4_3312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014095SMESG000013712.1 dd_Smed_v4_3312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014095SMESG000013712.1 dd_Smed_v4_3312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014095SMESG000013712.1 dd_Smed_v6_3312_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Nuclease vs. UniProt/SwissProt
Match: sp|P29769|NUC_SHIFL (Micrococcal nuclease OS=Shigella flexneri OX=623 GN=nuc PE=3 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.272e-17
Identity = 45/152 (29.61%), Postives = 78/152 (51.32%), Query Frame = 2 
Query:  548 GNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYL---------SVKDLDINKAMIDSGYAWCYRYTKCPVSYQ--ESMKLAKTLRKGLFQNKNPTTPWDWRKSN 970
             +++  + +++DGDT+   +    N+  I++R+  IDAPE  Q  G  A+ +L  +  +   + VQ+  ++ D YGR++G +           +  +IN  M+  G AW YRY   P   Q  E  K A+  R GL+ + N   PW WR+++
Sbjct:   25 ADFRGEVVRILDGDTIDVLV----NRQTIRVRLADIDAPESGQAFGSRARQRLADLTFR---QEVQVTEKEVDRYGRTLGVVYAPLQYPGGQTQLTNINAIMVQEGMAWAYRYYGKPTDAQMYEYEKEARRQRLGLWSDPNAQEPWKWRRAS 169
[Close]
BLAST of Nuclease vs. UniProt/SwissProt
Match: sp|Q2QYR1|CAN3_ORYSJ (Probable staphylococcal-like nuclease CAN3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0109200 PE=2 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 4.057e-17
Identity = 45/126 (35.71%), Postives = 78/126 (61.90%), Query Frame = 2 
Query:  602 KLLNSSNKSII----KIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYR-YTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRK 964
            K+++ S+  ++    +IRM GIDAPE+K  +G+E++N L ++I     K+V++ V D D +GR +G +   +L I + M+ +G+AW ++ Y K P  +    + AK   +GL+ + NP  PWDWR+
Sbjct:  243 KIISCSDDEVLARKYRIRMRGIDAPELKMPYGRESRNALVKLI---GGKSVKIYVYDLDQFGRYVGDIYCNNLFIQEQMLKNGHAWHFKTYDKRP-EFARWEREAKAANRGLWASGNPEKPWDWRR 364
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BLAST of Nuclease vs. UniProt/SwissProt
Match: sp|F4IH31|CAN2_ARATH (Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.445e-16
Identity = 53/141 (37.59%), Postives = 81/141 (57.45%), Query Frame = 2 
Query:  572 KVIDGDTVYAKLLNSSNKSII----KIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCY-RYTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRKSNPRK 979
            K+ID      ++LN  N+ ++    +IR+ GIDAPE +   GKEA+  L +I+ +   K++++ V   D YGR +G L    + + +AM+  G AW Y  Y K PV   +  K A+  R GL+ + NP  PWDWRK+N R+
Sbjct:  199 KIIDSGY---RVLNIENEEVLARKFRIRLRGIDAPESQMPFGKEAQEGLLKIVGR---KSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKRPV-LAKWEKEARQKRIGLWASSNPEKPWDWRKNNRRE 332
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BLAST of Nuclease vs. UniProt/SwissProt
Match: sp|Q2RBJ2|CAN2_ORYSJ (Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0109500 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.124e-16
Identity = 44/126 (34.92%), Postives = 77/126 (61.11%), Query Frame = 2 
Query:  602 KLLNSSNKSII----KIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYR-YTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRK 964
            K+++ S+  ++    +IRM GIDAPE+K  +GKE++  L ++I     K+V++ V D D +GR +G +   +L I + M+ +G+AW ++ Y K P  +    + A+   +GL+ + NP  PWDWR+
Sbjct:  234 KIISCSDDEVLARKYRIRMRGIDAPELKMPYGKESRTALVKLI---GGKSVKIYVYDLDQFGRYVGDIYCNNLFIQEQMLKNGHAWHFKTYDKRP-EFARWEREARAANRGLWASGNPEKPWDWRR 355
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BLAST of Nuclease vs. UniProt/SwissProt
Match: sp|P22997|PARB4_ECOLX (Protein ParB OS=Escherichia coli OX=562 GN=parB PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.199e-15
Identity = 46/152 (30.26%), Postives = 84/152 (55.26%), Query Frame = 2 
Query:  566 ITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSV---------KDLDINKAMIDSGYAWCYRYTKCPVSYQESMKL---AKTLRKGLFQNKNPTTPWDWRK-SNPRKN 982
            + ++IDGDT+   +    +K  +++R+  IDAPE +Q  G+ A+  L  ++ +   ++V +  +DTD YGR++G + V         +  ++N AM+  G AW YR+     +  E ++L   A+  R GL+ + +   PW WR+ SN R++
Sbjct:  136 VVRIIDGDTIDVLV----DKQPVRVRLVDIDAPEKRQAFGERARQALAGMVFR---RHVLVDEKDTDRYGRTLGTVWVNMELASRPPQPRNVNAAMVHQGMAWAYRFHGR-AADPEMLRLEQEARGKRVGLWSDPHAVEPWKWRRESNNRRD 279
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BLAST of Nuclease vs. TrEMBL
Match: A0A5B9ASH2 (Thermonuclease family protein OS=Escherichia coli OX=562 GN=FTV93_25545 PE=4 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 2.708e-27
Identity = 66/153 (43.14%), Postives = 92/153 (60.13%), Query Frame = 2 
Query:  542 SRGN---YKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPV--SYQESMKLAKTLRKGLFQNKNPTTPWDWRKSN--PRK 979
            SRGN   ++  + K++DGDT+ A  L++ N +I KIRM GIDAPE KQ  G++AK  L   I   + K V +    TDIYGR +G + +   DIN +M+DSGYAW YR+    +   Y +    A+   KGL+ + NP  PW WR++N  PRK
Sbjct:   20 SRGNSTTFEAKVVKIVDGDTITA--LDAQNTTI-KIRMYGIDAPESKQAFGQKAKQALTTAI---ATKIVTVIDHGTDIYGRMLGTIWLDGYDINASMVDSGYAWVYRFEDNAIVPGYIKYESAAQKEAKGLWADTNPVPPWQWRQANEKPRK 166
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BLAST of Nuclease vs. TrEMBL
Match: A0A0G3WJN3 (Putative nuclease OS=Endomicrobium proavitum OX=1408281 GN=Epro_0711 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 4.804e-27
Identity = 68/167 (40.72%), Postives = 95/167 (56.89%), Query Frame = 2 
Query:  473 GFFKENTAFKSTLKPKEKKIEKLSRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCY-RYTKCP--VSYQESMKLAKTLRKGLFQNKNPTTPWDWRK 964
            GFF + T  K   +P         +G     +TK+ DGDT+   ++NS NK ++KIR+ GIDAPE KQ  G  +K  L   I   + K + + V D D Y RS+G + + DLDIN+ M+ +GYAW Y +Y K P    ++    +AK  +KGL+Q  NPT PW WRK
Sbjct:   25 GFFAKETVVKDYAEP--------VKGVIAGKVTKIADGDTL--TIINSDNK-LVKIRLYGIDAPETKQEFGSNSKQFLSSQI---NGKYITVDVIDIDKYKRSVGRVFIDDLDINREMVKNGYAWVYSQYMKLPEKSEWENLQTIAKADKKGLWQEDNPTAPWKWRK 177
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BLAST of Nuclease vs. TrEMBL
Match: A0A2C8CVE4 (Protein ParB OS=Yersinia pestis OX=632 GN=yhcR PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 5.365e-27
Identity = 62/150 (41.33%), Postives = 91/150 (60.67%), Query Frame = 2 
Query:  542 SRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPV--SYQESMKLAKTLRKGLFQNKNPTTPWDWRKSN--PRK 979
             + +++  + K+IDGDT+ A  L+  N SI KIRM GIDAPE KQ  G++AK  L   I   + +N+ +     DIYGR +G + +   DIN +M+DSGYAW YR+   P+  +Y +    A+   KGL+ + NP  PW+WR+ N  P+K
Sbjct:   23 GQSSFEAKVVKIIDGDTITA--LDGQNTSI-KIRMYGIDAPESKQAFGQKAKQALSSAI---AAQNITVIDHGPDIYGRMLGTIWLDGYDINASMVDSGYAWVYRFDGNPIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQNQKPQK 166
[Close]
BLAST of Nuclease vs. TrEMBL
Match: V0AWS4 (Nuclease-like protein OS=Escherichia coli 909945-2 OX=1269007 GN=HMPREF1620_00828 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 5.365e-27
Identity = 62/150 (41.33%), Postives = 91/150 (60.67%), Query Frame = 2 
Query:  542 SRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPV--SYQESMKLAKTLRKGLFQNKNPTTPWDWRKSN--PRK 979
             + +++  + K+IDGDT+ A  L+  N SI KIRM GIDAPE KQ  G++AK  L   I   + +N+ +     DIYGR +G + +   DIN +M+DSGYAW YR+   P+  +Y +    A+   KGL+ + NP  PW+WR+ N  P+K
Sbjct:   23 GQSSFEAKVVKIIDGDTITA--LDGQNTSI-KIRMYGIDAPESKQAFGQKAKQALSSAI---AAQNITVIDHGPDIYGRMLGTIWLDGYDINASMVDSGYAWVYRFDGNPIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQNQKPQK 166
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BLAST of Nuclease vs. TrEMBL
Match: A0A2Z3XDH0 (Thermonuclease family protein OS=Citrobacter sp. CRE-46 OX=1703250 GN=AN232_26610 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 5.365e-27
Identity = 62/150 (41.33%), Postives = 91/150 (60.67%), Query Frame = 2 
Query:  542 SRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPV--SYQESMKLAKTLRKGLFQNKNPTTPWDWRKSN--PRK 979
             + +++  + K+IDGDT+ A  L+  N SI KIRM GIDAPE KQ  G++AK  L   I   + +N+ +     DIYGR +G + +   DIN +M+DSGYAW YR+   P+  +Y +    A+   KGL+ + NP  PW+WR+ N  P+K
Sbjct:   23 GQSSFEAKVVKIIDGDTITA--LDGQNTSI-KIRMYGIDAPESKQAFGQKAKQALSSAI---AAQNITVIDHGPDIYGRMLGTIWLDGYDINASMVDSGYAWVYRFDGNPIVPNYLKFEASAQKAVKGLWVDPNPVAPWEWRQQNQKPQK 166
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BLAST of Nuclease vs. Planmine SMEST
Match: SMESG000013712.1 (SMESG000013712.1)

HSP 1 Score: 613.994 bits (1582), Expect = 0.000e+0
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 2 
Query:    2 MKLNXXXXXXXXXXXYEVISESHRKGKCRLTEEDESCSRVKIVKIINGNTVNLVFAKDRNQSVRTLHLYGIETPKLNESFGIEAXXXXXXXXXXXXXXSARIRKERTASKDLIGTLYSKRIDINKKMITSGHAWCKNPKTCPKSYYKATQRSMKHHKGFFKENTAFKSTLKPKEKKIEKLSRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRKSNPRKN 982
            MKLNLLFFFFVFVLFYEVISESHRKGKCRLTEEDESCSRVKIVKIINGNTVNLVFAKDRNQSVRTLHLYGIETPKLNESFGIEAKERLSKILKKKKHLSARIRKERTASKDLIGTLYSKRIDINKKMITSGHAWCKNPKTCPKSYYKATQRSMKHHKGFFKENTAFKSTLKPKEKKIEKLSRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRKSNPRKN
Sbjct:    1 MKLNLLFFFFVFVLFYEVISESHRKGKCRLTEEDESCSRVKIVKIINGNTVNLVFAKDRNQSVRTLHLYGIETPKLNESFGIEAKERLSKILKKKKHLSARIRKERTASKDLIGTLYSKRIDINKKMITSGHAWCKNPKTCPKSYYKATQRSMKHHKGFFKENTAFKSTLKPKEKKIEKLSRGNYKITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRKSNPRKN 327
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BLAST of Nuclease vs. Planmine SMEST
Match: SMESG000064715.1 (SMESG000064715.1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.808e-22
Identity = 51/137 (37.23%), Postives = 82/137 (59.85%), Query Frame = 2 
Query:  560 ITITKVIDGDTVYAKLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLKVEDTDIYGRSIGYLS-VKDLDINKAMIDSGYAWCYRYTKCPVSYQESMKLAKTLRKGLFQNKNPTTPWDWRKS 967
            + +  V DGDT+    + +SN + I++R   IDAPE  Q  G E+ +KL  +++   N+          IYGR +  ++ +  LD+N+ M+  GYAWC+R  +CP  Y++  KLAK  + GL++N NP +PW++RKS
Sbjct:   31 VKLHHVSDGDTI---TVLASNGTKIRVRFAFIDAPERNQEFGVESTSKLHDLLK---NEPYFKVFRYHTIYGRVLAKVTTLAGLDLNREMVKDGYAWCFR-RRCPKEYRQLQKLAKKNQIGLWKNPNPRSPWNYRKS 160
The following BLAST results are available for this feature:
BLAST of Nuclease vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P29769|NUC_SHIFL1.272e-1729.61Micrococcal nuclease OS=Shigella flexneri OX=623 G... [more]
sp|Q2QYR1|CAN3_ORYSJ4.057e-1735.71Probable staphylococcal-like nuclease CAN3 OS=Oryz... [more]
sp|F4IH31|CAN2_ARATH1.445e-1637.59Staphylococcal-like nuclease CAN2 OS=Arabidopsis t... [more]
sp|Q2RBJ2|CAN2_ORYSJ3.124e-1634.92Probable staphylococcal-like nuclease CAN2 OS=Oryz... [more]
sp|P22997|PARB4_ECOLX7.199e-1530.26Protein ParB OS=Escherichia coli OX=562 GN=parB PE... [more]
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BLAST of Nuclease vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5B9ASH22.708e-2743.14Thermonuclease family protein OS=Escherichia coli ... [more]
A0A0G3WJN34.804e-2740.72Putative nuclease OS=Endomicrobium proavitum OX=14... [more]
A0A2C8CVE45.365e-2741.33Protein ParB OS=Yersinia pestis OX=632 GN=yhcR PE=... [more]
V0AWS45.365e-2741.33Nuclease-like protein OS=Escherichia coli 909945-2... [more]
A0A2Z3XDH05.365e-2741.33Thermonuclease family protein OS=Citrobacter sp. C... [more]
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BLAST of Nuclease vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Nuclease vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000013712.10.000e+0100.00SMESG000013712.1[more]
SMESG000064715.11.808e-2237.23SMESG000064715.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014095 ID=SMED30014095|Name=Nuclease|organism=Schmidtea mediterranea sexual|type=transcript|length=1000bp
TATGAAATTAAATTTATTGTTTTTCTTTTTTGTGTTTGTTTTATTTTACG
AAGTTATATCAGAATCACACAGAAAGGGAAAATGTCGATTAACTGAGGAA
GACGAGTCTTGTTCTCGAGTTAAAATAGTGAAAATTATCAACGGGAACAC
TGTCAACTTGGTCTTTGCAAAAGATAGAAACCAGAGCGTGAGAACATTAC
ATCTCTACGGAATTGAAACACCAAAATTAAATGAAAGTTTTGGAATTGAA
GCTAAAGAAAGATTGAGTAAAATTCTTAAAAAGAAAAAACATTTATCAGC
CAGAATCAGAAAAGAAAGAACAGCTAGTAAAGATTTAATAGGAACGTTGT
ATTCCAAAAGGATTGACATTAATAAAAAAATGATCACTAGTGGACATGCT
TGGTGCAAAAATCCAAAGACCTGCCCAAAAAGTTACTATAAAGCTACCCA
ACGATCAATGAAACACCATAAAGGATTTTTTAAAGAAAATACAGCTTTTA
AATCTACACTAAAGCCAAAAGAAAAAAAAATTGAAAAACTTTCGAGAGGA
AATTACAAAATTACAATTACTAAAGTAATTGATGGAGACACTGTCTATGC
AAAATTGCTGAATTCTTCAAATAAATCCATTATAAAAATAAGAATGGGAG
GAATTGATGCTCCAGAAATGAAACAATTGCATGGAAAAGAGGCAAAAAAT
AAATTAGAACAAATAATCGAAAAGTTTTCCAATAAAAACGTTCAATTAAA
AGTTGAAGATACGGATATCTACGGAAGAAGTATTGGTTACTTGAGCGTTA
AAGATCTTGATATTAACAAAGCAATGATTGACAGTGGCTATGCTTGGTGT
TACAGGTATACCAAATGTCCAGTCTCATATCAGGAATCTATGAAGCTCGC
CAAAACTTTACGCAAAGGATTATTTCAAAACAAAAATCCAACGACTCCAT
GGGACTGGAGAAAATCAAATCCAAGAAAAAATTAACATTCTTAATTAGGA
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protein sequence of SMED30014095-orf-1

>SMED30014095-orf-1 ID=SMED30014095-orf-1|Name=SMED30014095-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=328bp
MKLNLLFFFFVFVLFYEVISESHRKGKCRLTEEDESCSRVKIVKIINGNT
VNLVFAKDRNQSVRTLHLYGIETPKLNESFGIEAKERLSKILKKKKHLSA
RIRKERTASKDLIGTLYSKRIDINKKMITSGHAWCKNPKTCPKSYYKATQ
RSMKHHKGFFKENTAFKSTLKPKEKKIEKLSRGNYKITITKVIDGDTVYA
KLLNSSNKSIIKIRMGGIDAPEMKQLHGKEAKNKLEQIIEKFSNKNVQLK
VEDTDIYGRSIGYLSVKDLDINKAMIDSGYAWCYRYTKCPVSYQESMKLA
KTLRKGLFQNKNPTTPWDWRKSNPRKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR035437SNase_OB-fold_sf
IPR016071Staphylococal_nuclease_OB-fold
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 232..252
NoneNo IPR availablePANTHERPTHR12302EBNA2 BINDING PROTEIN P100coord: 189..322
NoneNo IPR availablePANTHERPTHR12302EBNA2 BINDING PROTEIN P100coord: 44..172
NoneNo IPR availablePANTHERPTHR12302:SF3ENDONUCLEASE LCL3-RELATEDcoord: 44..172
coord: 189..322
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.866
NoneNo IPR availableSUPERFAMILYSSF50199Staphylococcal nucleasecoord: 185..317
NoneNo IPR availableSUPERFAMILYSSF50199Staphylococcal nucleasecoord: 35..163
IPR016071Staphylococcal nuclease (SNase-like), OB-foldSMARTSM00318SNASE_2coord: 183..310
e-value: 2.7E-17
score: 73.5
coord: 36..162
e-value: 3.0E-4
score: 15.7
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPFAMPF00565SNasecoord: 212..308
e-value: 6.0E-15
score: 55.6
coord: 66..158
e-value: 9.4E-9
score: 35.7
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPROSITEPS50830TNASE_3coord: 183..310
score: 26.998
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPROSITEPS50830TNASE_3coord: 36..162
score: 16.865
IPR035437SNase-like, OB-fold superfamilyGENE3DG3DSA:2.40.50.90coord: 31..167
e-value: 1.6E-15
score: 59.2
coord: 177..315
e-value: 7.7E-27
score: 96.0