Ast5 protein

Overview
NameAst5 protein (CCW36843.1)
Smed IDSMED30014093
Length (bp)889
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ast5 protein (SMED30014093) t-SNE clustered cells

Violin plots show distribution of expression levels for Ast5 protein (SMED30014093) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ast5 protein (SMED30014093) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ast5 protein (SMED30014093) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30014093

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30014093h1SMcG0018135 SmedASXL_007868SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30014093h1SMcG0018135 SmedASXL_007868SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30014093h1SMcG0018135 OX_Smed_1.0.09924ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ast5 protein vs. Ensembl Human
Match: MEP1A (meprin A subunit alpha [Source:HGNC Symbol;Acc:HGNC:7015])

HSP 1 Score: 73.1738 bits (178), Expect = 8.549e-14
Identity = 51/123 (41.46%), Postives = 64/123 (52.03%), Query Frame = 1 
Query:  418 LKGCVKY--VESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEET---VTYKTNSVMHYPSESF 771
            LK CV +   E  S Y I  Q    GCWS VG    Q   Q +++   GC Y K  I+HE +HA GF H Q R DRD +VNI+W++I S + H F T  +   T     Y   S+MHY   SF
Sbjct:  132 LKSCVDFKPYEGESSYIIFQQFD--GCWSEVG---DQHVGQNISI-GQGCAY-KAIIEHEILHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSF 247
[Close]
BLAST of Ast5 protein vs. Ensembl Human
Match: MEP1A (meprin A subunit alpha [Source:HGNC Symbol;Acc:HGNC:7015])

HSP 1 Score: 73.1738 bits (178), Expect = 9.003e-14
Identity = 51/123 (41.46%), Postives = 64/123 (52.03%), Query Frame = 1 
Query:  418 LKGCVKY--VESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEET---VTYKTNSVMHYPSESF 771
            LK CV +   E  S Y I  Q    GCWS VG    Q   Q +++   GC Y K  I+HE +HA GF H Q R DRD +VNI+W++I S + H F T  +   T     Y   S+MHY   SF
Sbjct:  104 LKSCVDFKPYEGESSYIIFQQFD--GCWSEVG---DQHVGQNISI-GQGCAY-KAIIEHEILHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSF 219
[Close]
BLAST of Ast5 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 72.4034 bits (176), Expect = 1.357e-13
Identity = 43/98 (43.88%), Postives = 55/98 (56.12%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKN---CEETVTYKTNSVMHYPSESF 771
            GCWS VG  N++ G QEL++    C     T++HEF+HA GF H Q R+DRD +V I W+RI S   H F T  +       V Y   SVMHY   +F
Sbjct:  123 GCWSSVG--NRRVGKQELSI-GANC-DRIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAF 216
[Close]
BLAST of Ast5 protein vs. Ensembl Human
Match: MEP1B (meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC:7020])

HSP 1 Score: 72.4034 bits (176), Expect = 1.395e-13
Identity = 43/98 (43.88%), Postives = 55/98 (56.12%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKN---CEETVTYKTNSVMHYPSESF 771
            GCWS VG  N++ G QEL++    C     T++HEF+HA GF H Q R+DRD +V I W+RI S   H F T  +       V Y   SVMHY   +F
Sbjct:  123 GCWSSVG--NRRVGKQELSI-GANC-DRIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMHYSKTAF 216
[Close]
BLAST of Ast5 protein vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 66.2402 bits (160), Expect = 1.127e-11
Identity = 39/131 (29.77%), Postives = 63/131 (48.09%), Query Frame = 1 
Query:  367 QTKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLY-SKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHY 756
            +  +Q++ E++ E       C+++V               GC+S VG   + GG Q ++L  P CL   +G + HE +H  GF H   RADRD+++ + W  I   F   F+  ++      Y  +SVMHY
Sbjct:  114 EPSRQVILEALAEFERST--CIRFVTYQDQRDFISIIPMYGCFSSVG---RSGGMQVVSLA-PTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHY 238
[Close]
BLAST of Ast5 protein vs. Ensembl Celegans
Match: nas-13 (Zinc metalloproteinase nas-13 [Source:UniProtKB/Swiss-Prot;Acc:Q20191])

HSP 1 Score: 74.3294 bits (181), Expect = 1.088e-14
Identity = 50/135 (37.04%), Postives = 68/135 (50.37%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAF--VTCKNCEETVT-YKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            GC+S VG +   GG Q ++L + GC+  KG I HE +HA GF H Q RADRD++V I W  +++     F   +    +   T Y   SVMHY   +F   G+      ++N EI    G  E D   I  LY C
Sbjct:  173 GCYSLVGRI---GGKQPVSLGD-GCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEAGLQDQFDKYSLNMIDHLGTKYDYGSVMHYAPTAFSKNGKPTIEPIEKNVEIGQRAGFSENDIYKINMLYNC 302
[Close]
BLAST of Ast5 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 73.1738 bits (178), Expect = 1.456e-14
Identity = 55/177 (31.07%), Postives = 83/177 (46.89%), Query Frame = 1 
Query:  376 KQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQP--GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERI----DSDF-CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEE-DHQMIKHLYQC 882
            + ++  +MNE     K CVK+V      H       P  GC+S VG   K GG Q ++L++ GC+   GTI HE +HA GF H Q R DRD ++++ W+ +    D  F  +      + +E   Y   S+MHY   +F   G++  +  K   E         + D + I  LY C
Sbjct:  122 RSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPDEGCYSLVG---KTGGKQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYA--SIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast5 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 72.4034 bits (176), Expect = 3.280e-14
Identity = 55/177 (31.07%), Postives = 83/177 (46.89%), Query Frame = 1 
Query:  376 KQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQP--GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERI----DSDF-CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEE-DHQMIKHLYQC 882
            + ++  +MNE     K CVK+V      H       P  GC+S VG   K GG Q ++L++ GC+   GTI HE +HA GF H Q R DRD ++++ W+ +    D  F  +      + +E   Y   S+MHY   +F   G++  +  K   E         + D + I  LY C
Sbjct:  122 RSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPDEGCYSLVG---KTGGKQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYA--SIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast5 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 72.0182 bits (175), Expect = 3.493e-14
Identity = 55/177 (31.07%), Postives = 83/177 (46.89%), Query Frame = 1 
Query:  376 KQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQP--GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERI----DSDF-CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEE-DHQMIKHLYQC 882
            + ++  +MNE     K CVK+V      H       P  GC+S VG   K GG Q ++L++ GC+   GTI HE +HA GF H Q R DRD ++++ W+ +    D  F  +      + +E   Y   S+MHY   +F   G++  +  K   E         + D + I  LY C
Sbjct:  122 RSVIANAMNEYHT--KTCVKFVARDPSKHHDYLWIHPDEGCYSLVG---KTGGKQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYA--SIMHYGPYAFSGSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
[Close]
BLAST of Ast5 protein vs. Ensembl Celegans
Match: nas-15 (Zinc metalloproteinase nas-15 [Source:UniProtKB/Swiss-Prot;Acc:P55115])

HSP 1 Score: 71.633 bits (174), Expect = 8.199e-14
Identity = 56/174 (32.18%), Postives = 82/174 (47.13%), Query Frame = 1 
Query:  382 IVRESMNELTNDLKGCVKYV--ESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETV-----TYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            ++  SM E  +    C+++V  E+    ++ +   + GC+S VG   K GG Q L+L + GC+  KG I HE +HA GF H Q R DRD  + I W  I +     F   K    T+      Y   S+MHY +++F   GQ   +  K    I   +G  + D   I  LY C
Sbjct:  143 LIAASMQEYASHT--CIRWVPKEAADVNYVHIYPDR-GCYSMVG---KMGGKQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDDHITIMWNNIQAGMQGQFE--KYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGATIGQRNGFSKVDKFKINTLYGC 306
[Close]
BLAST of Ast5 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 97.8265 bits (242), Expect = 3.799e-23
Identity = 54/158 (34.18%), Postives = 83/158 (52.53%), Query Frame = 1 
Query:  421 KGCVKYVESTSGY-HIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEE-TVTYKTNSVMHYPSESFPCPGQRITMVDKQN--KEIPYNHGTIEEDHQMIKHLYQC 882
            + C+++V+ +S   +I ++    GCWS VG   + GG QE+NL++PGCL   GT  HE +HA GF+H Q R +RD +V I +  + S   + F      E   V Y   SVMHY   +F   GQ   +  + N   ++   +G  + D Q +  +Y C
Sbjct:  148 RTCIRFVKRSSERDYISIRGDNSGCWSSVG---RVGGKQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302
[Close]
BLAST of Ast5 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 97.8265 bits (242), Expect = 3.799e-23
Identity = 54/158 (34.18%), Postives = 83/158 (52.53%), Query Frame = 1 
Query:  421 KGCVKYVESTSGY-HIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEE-TVTYKTNSVMHYPSESFPCPGQRITMVDKQN--KEIPYNHGTIEEDHQMIKHLYQC 882
            + C+++V+ +S   +I ++    GCWS VG   + GG QE+NL++PGCL   GT  HE +HA GF+H Q R +RD +V I +  + S   + F      E   V Y   SVMHY   +F   GQ   +  + N   ++   +G  + D Q +  +Y C
Sbjct:  148 RTCIRFVKRSSERDYISIRGDNSGCWSSVG---RVGGKQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGADKMGQRNGFSDYDIQKLNRMYDC 302
[Close]
BLAST of Ast5 protein vs. Ensembl Fly
Match: CG5715 (gene:FBgn0039180 transcript:FBtr0084634)

HSP 1 Score: 85.1149 bits (209), Expect = 7.018e-19
Identity = 54/162 (33.33%), Postives = 79/162 (48.77%), Query Frame = 1 
Query:  421 KGCVKY-VESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEE---TVTYKTNSVMHYPSESFPCPGQ---RITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            K CV++   +T   +I + S + GCWS +G L   GG QE+NL++P CL + GT  HE +HA GF H Q R +RD +V +  + I  +    F    +  +    V Y   SVMHY   SF   GQ   +         ++    G    D + I  +Y+CK
Sbjct:  136 KTCVRFKPRTTEKDYISIGSGKSGCWSSIGRL---GGRQEVNLQSPNCLRTYGTPIHELMHALGFFHEQNRHERDSYVRVMKDNIKPEMMVNFEKSSSRTQYGFGVEYDYGSVMHYSPTSFTRNGQPTLKALRATSDASQMGQRKGFSAGDVRKINAMYKCK 294
[Close]
BLAST of Ast5 protein vs. Ensembl Fly
Match: CG15253 (gene:FBgn0028948 transcript:FBtr0080782)

HSP 1 Score: 82.8037 bits (203), Expect = 2.573e-18
Identity = 60/165 (36.36%), Postives = 85/165 (51.52%), Query Frame = 1 
Query:  427 CVKYVESTSG--YHIKVQSHQPGCWSFVGMLNKQGGSQELNLEN----PGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVT-----YKTNSVMHYPSESFPCPGQRITMVDKQNKE--IPYNHGTIEEDHQMIKHLYQC 882
            C+ + E+T+   Y++ V S + GC+S++G LN+    Q+LNL+N     GC +   TI HEF+HA GF H Q  ADRD +V I  E I       F   K  EETV      Y   SVMHY   +F   G+R  +  ++ KE  I       E D + +  +Y+C
Sbjct:   90 CLTFKEATTDQKYYVNVTSEEGGCFSYIGYLNR---VQQLNLQNNEIGVGC-FRLYTIVHEFLHALGFFHQQSAADRDDYVQIVEENITEGMEFNF--DKYTEETVNDFGEKYDYGSVMHYGPYAFSKNGERTILALEEGKEDVIGQRLELSETDIRKLNAIYKC 248
[Close]
BLAST of Ast5 protein vs. Ensembl Fly
Match: CG10280 (gene:FBgn0037395 transcript:FBtr0309318)

HSP 1 Score: 78.9518 bits (193), Expect = 2.192e-16
Identity = 43/97 (44.33%), Postives = 54/97 (55.67%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPG-----CLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAF--VTCKNCEETVTYKTNSVMHY 756
            GCWS+VG   ++GG Q ++L+ P      C  S+G I HE +HA G  H Q RADRD FV I W+ I   F   F  V+ K  +    Y  NSVMHY
Sbjct:  207 GCWSYVG---RRGGEQVVSLQRPDENSAHCFSSEGRIMHELMHAIGIYHEQSRADRDNFVKIHWDNIVPRFRKNFKLVSKKKGKYAFDYDYNSVMHY 300
[Close]
BLAST of Ast5 protein vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 96.2857 bits (238), Expect = 2.110e-23
Identity = 58/154 (37.66%), Postives = 82/154 (53.25%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            CVK+V  T   +      + GCWS++GM    GGSQ ++L++PGC++S G   HE +HA GF+H Q R+DRD++V+I WE I  +  H F   +       Y  +SVMHY   +F   G   T++ K +  IP     G    D   I  LY C
Sbjct:   67 CVKFVPRTHQANFLNIQPRYGCWSYLGM---TGGSQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFSEDGGP-TIIPKPDPYIPIGQRDGPSILDIHKINILYNC 215
[Close]
BLAST of Ast5 protein vs. Ensembl Zebrafish
Match: hce2l1 (high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:ZDB-GENE-070912-147])

HSP 1 Score: 96.6709 bits (239), Expect = 4.767e-23
Identity = 58/154 (37.66%), Postives = 82/154 (53.25%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            CVK+V  T   +      + GCWS++GM    GGSQ ++L++PGC++S G   HE +HA GF+H Q R+DRD++V+I WE I  +  H F   +       Y  +SVMHY   +F   G   T++ K +  IP     G    D   I  LY C
Sbjct:  125 CVKFVPRTHQANFLNIQPRYGCWSYLGM---TGGSQTVSLQSPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVSILWENIIENQRHNFRKYETNNLNTAYDYSSVMHYGRYAFSEDGGP-TIIPKPDPYIPIGQRDGPSILDIHKINILYNC 273
[Close]
BLAST of Ast5 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 89.3521 bits (220), Expect = 2.178e-20
Identity = 58/168 (34.52%), Postives = 82/168 (48.81%), Query Frame = 1 
Query:  385 VRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            ++E M  +  D   CV++V  T          + GCWS++G    +GG Q ++L++P C  S G   HE +HA GF+H Q RADRDK+V I W  I  D    F   K       Y  +SVMH+   +F   G+  T+V K+N  +      G  + D   I  LY C
Sbjct:  113 IKEGMELIEQDT--CVRFVPRTHQRDYLDIQPKTGCWSYLG---ARGGRQTISLQSPDCTGS-GVTVHELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEP-TIVPKRNWNVKIGQRLGPSDLDIMKINKLYSC 273
[Close]
BLAST of Ast5 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 86.2705 bits (212), Expect = 1.088e-19
Identity = 57/166 (34.34%), Postives = 81/166 (48.80%), Query Frame = 1 
Query:  385 VRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLY 876
            ++E M  +  D   CV++V  T          + GCWS++G    +GG Q ++L++P C  S G   HE +HA GF+H Q RADRDK+V I W  I  D    F   K       Y  +SVMH+   +F   G+  T+V K+N  +      G  + D   I  LY
Sbjct:   54 IKEGMELIEQDT--CVRFVPRTHQRDYLDIQPKTGCWSYLGA---RGGRQTISLQSPDCTGS-GVTVHELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEP-TIVPKRNWNVKIGQRLGPSDLDIMKINKLY 212
[Close]
BLAST of Ast5 protein vs. Ensembl Zebrafish
Match: he1.2 (hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;Acc:ZDB-GENE-030131-2100])

HSP 1 Score: 87.0409 bits (214), Expect = 1.164e-19
Identity = 52/155 (33.55%), Postives = 81/155 (52.26%), Query Frame = 1 
Query:  427 CVKYV-ESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPC-PG-QRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            C+++V  S    ++ ++ ++ GC+S +G   + GG Q ++L   GC+YS G  +HE  HA GF H Q R+DRD++V I W  I     + F+  K   +   Y   S+MHY   +F   PG + IT +  +N +I    G  + D   I  LY C
Sbjct:  114 CIRFVPRSIQADYLSIE-NKDGCYSAIG---RTGGKQVVSLNRKGCVYS-GIAQHELNHALGFYHEQSRSDRDQYVRINWNNISPGMAYNFLKQKTNNQNTPYDYGSLMHYGKTAFAIQPGLETITPIPDENVQIGQRQGLSKIDILRINKLYGC 263
[Close]
BLAST of Ast5 protein vs. Ensembl Xenopus
Match: ENSXETT00000022029.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496745])

HSP 1 Score: 94.3597 bits (233), Expect = 3.439e-21
Identity = 57/174 (32.76%), Postives = 94/174 (54.02%), Query Frame = 1 
Query:  370 TKKQIVRESMNELTNDLKGCVKYVE-STSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKE--IPYNHGTIEEDHQMIKHLYQC 882
            T++ I+R +M+E+T  +  C+++V  +    +++++ +  GCWS++G   + GG+Q+++L   GCL+  G I+HE +H+ GF H Q R+DRD ++NI W+ I  D    F+          Y   SVMHY   +F     + T+  K N    I    G    D + I  LYQC
Sbjct:   45 TERDIIRAAMDEVT--VLTCIQFVTYNNESDYLRIRPYD-GCWSYIG---RVGGAQDVSLMKTGCLH-HGVIQHELLHSLGFQHEQCRSDRDNYININWDNISHDKERNFLKMNTQNLGSPYDYLSVMHYGKFAFATNSGKPTLEPKGNPSAMIGQRVGLSSLDVEKINRLYQC 211
[Close]
BLAST of Ast5 protein vs. Ensembl Xenopus
Match: ENSXETT00000006778.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496122])

HSP 1 Score: 94.3597 bits (233), Expect = 4.692e-21
Identity = 55/155 (35.48%), Postives = 75/155 (48.39%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYN--HGTIEEDHQMIKHLYQCK 885
            C+++V  T+           GCWSF+G     GG Q+L+L+  GCL S G I+HE  HA GF+H   R+DRD +V IFW  I  ++  +F       + + Y   SVMHY   S+    Q  T+    N  IP    +G    D   I  LY C 
Sbjct:  126 CIRFVPHTNERDFLNIISDSGCWSFLG--RAGGGGQDLSLQRGGCL-SNGIIQHELNHALGFVHEHTRSDRDSYVKIFWNNIQPEYKDSFNKTDTDNQGMEYDYGSVMHYGRNSYSIDYQLPTIQPIPNGLIPIGQRYGLSSLDAAKINRLYNCS 277
[Close]
BLAST of Ast5 protein vs. Ensembl Xenopus
Match: ENSXETT00000006820.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496122])

HSP 1 Score: 94.3597 bits (233), Expect = 4.901e-21
Identity = 55/155 (35.48%), Postives = 75/155 (48.39%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYN--HGTIEEDHQMIKHLYQCK 885
            C+++V  T+           GCWSF+G     GG Q+L+L+  GCL S G I+HE  HA GF+H   R+DRD +V IFW  I  ++  +F       + + Y   SVMHY   S+    Q  T+    N  IP    +G    D   I  LY C 
Sbjct:  126 CIRFVPHTNERDFLNIISDSGCWSFLG--RAGGGGQDLSLQRGGCL-SNGIIQHELNHALGFVHEHTRSDRDSYVKIFWNNIQPEYKDSFNKTDTDNQGMEYDYGSVMHYGRNSYSIDYQLPTIQPIPNGLIPIGQRYGLSSLDAAKINRLYNCS 277
[Close]
BLAST of Ast5 protein vs. Ensembl Xenopus
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:Xenbase;Acc:XB-GENE-1011058])

HSP 1 Score: 90.8929 bits (224), Expect = 6.666e-20
Identity = 57/154 (37.01%), Postives = 75/154 (48.70%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            CV++V  T            GC S VG   + GG Q++ L + GC+ S+G I+HE  HA GF H  MR+DRD +V I  E I   + + F   K     + Y  NSVMHY   +F     + T+V K +  IP     G    D   IK LYQC
Sbjct:  112 CVRFVPKTIQRDFLNIVSNGGCLSMVG---RNGGGQKVELASYGCM-SRGVIQHELNHALGFYHEHMRSDRDDYVTIITENIIPSYENYFSKRKTNNMGIIYDYNSVMHYSRNTFSISPDKSTIVPKPDPSIPIGQRDGLSILDILKIKKLYQC 261
[Close]
BLAST of Ast5 protein vs. Ensembl Xenopus
Match: golt1b (golgi transport 1B [Source:Xenbase;Acc:XB-GENE-5753086])

HSP 1 Score: 90.1225 bits (222), Expect = 1.097e-19
Identity = 58/176 (32.95%), Postives = 89/176 (50.57%), Query Frame = 1 
Query:  376 KQIVRESMNELTNDLK-----GCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGT--IEEDHQMIKHLYQC 882
            KQ   + +N +T+ ++      CV++V  T        +   GCWS++G   +QGG+Q +++E   C  S+GT  HE  HA GF+H Q R+DRD +V+I ++ I       F   +      TY  +S+MHYP+ +F     + T+V K N   P   G+     D   I  LYQC
Sbjct:   94 KQYSSDQINTITSAMEVYSTLTCVQFVPYTDEEDYIAITSGDGCWSYMG---RQGGAQVVSVEKGYCT-SEGTTMHELNHALGFVHEQSRSDRDNYVDIMYQYISPGDIVNFKKMETNNLNTTYDYHSIMHYPAWAFSNTTGQNTIVAKLNPNTPLGPGSTMTNLDITKINRLYQC 265
[Close]
BLAST of Ast5 protein vs. Ensembl Mouse
Match: Mep1a (meprin 1 alpha [Source:MGI Symbol;Acc:MGI:96963])

HSP 1 Score: 75.0998 bits (183), Expect = 1.317e-14
Identity = 56/157 (35.67%), Postives = 80/157 (50.96%), Query Frame = 1 
Query:  418 LKGCVKY--VESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKN---CEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHL 873
            LK CV +   E  S Y I  +    GCWS +G  ++Q G Q +++   GC + K TI+HE +HA GF H Q R DRD +VNI+W++I +D+ H F T  +    +    Y   S+MHY   SF            +N+ IP     I E + +I  L
Sbjct:  116 LKSCVDFKPYEGESSYIIFQK--LSGCWSMIG--DQQVG-QNISI-GEGCDF-KATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSF-----------NKNESIPTITTKIPEFNTIIGQL 254
[Close]
BLAST of Ast5 protein vs. Ensembl Mouse
Match: Mep1a (meprin 1 alpha [Source:MGI Symbol;Acc:MGI:96963])

HSP 1 Score: 74.7146 bits (182), Expect = 1.518e-14
Identity = 56/157 (35.67%), Postives = 80/157 (50.96%), Query Frame = 1 
Query:  418 LKGCVKY--VESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKN---CEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHL 873
            LK CV +   E  S Y I  +    GCWS +G  ++Q G Q +++   GC + K TI+HE +HA GF H Q R DRD +VNI+W++I +D+ H F T  +    +    Y   S+MHY   SF            +N+ IP     I E + +I  L
Sbjct:  103 LKSCVDFKPYEGESSYIIFQK--LSGCWSMIG--DQQVG-QNISI-GEGCDF-KATIEHEILHALGFFHEQSRTDRDDYVNIWWDQIITDYEHNFNTYDDNTITDLNTPYDYESLMHYGPFSF-----------NKNESIPTITTKIPEFNTIIGQL 241
[Close]
BLAST of Ast5 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 68.5514 bits (166), Expect = 1.497e-12
Identity = 38/101 (37.62%), Postives = 54/101 (53.47%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPGCLY-SKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQ 786
            GC+S VG   + GG Q ++L  P CL   +G + HE +H  GF H   RADRD+++ + W  I   F   F+  ++    V Y  +SVMHY   +F   GQ
Sbjct:  152 GCFSGVG---RSGGMQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQ 248
[Close]
BLAST of Ast5 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 68.1662 bits (165), Expect = 1.781e-12
Identity = 38/101 (37.62%), Postives = 54/101 (53.47%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPGCLY-SKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQ 786
            GC+S VG   + GG Q ++L  P CL   +G + HE +H  GF H   RADRD+++ + W  I   F   F+  ++    V Y  +SVMHY   +F   GQ
Sbjct:  131 GCFSGVG---RSGGMQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQ 227
[Close]
BLAST of Ast5 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 68.1662 bits (165), Expect = 1.781e-12
Identity = 38/101 (37.62%), Postives = 54/101 (53.47%), Query Frame = 1 
Query:  487 GCWSFVGMLNKQGGSQELNLENPGCLY-SKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQ 786
            GC+S VG   + GG Q ++L  P CL   +G + HE +H  GF H   RADRD+++ + W  I   F   F+  ++    V Y  +SVMHY   +F   GQ
Sbjct:  131 GCFSGVG---RSGGMQVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQ 227
[Close]
BLAST of Ast5 protein vs. UniProt/SwissProt
Match: sp|P31580|HCE1_ORYLA (High choriolytic enzyme 1 OS=Oryzias latipes OX=8090 GN=hcea PE=1 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.833e-20
Identity = 55/157 (35.03%), Postives = 78/157 (49.68%), Query Frame = 1 
Query:  421 KGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQR--ITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            K C+++V  T+ Y       + GC+S +G   ++GG QEL++   GC+YS G I+HE  HA GF H Q R+DRD +V I WE I     + F           Y  +S+MHY  ++F     R  IT +   N  I   +G    D   I  LY C+
Sbjct:  118 KTCIRFVRRTNEYDFISVVSKTGCYSELG---RKGGQQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRINVLYNCR 270
[Close]
BLAST of Ast5 protein vs. UniProt/SwissProt
Match: sp|P31581|HCE2_ORYLA (High choriolytic enzyme 2 OS=Oryzias latipes OX=8090 GN=hceb PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 4.866e-19
Identity = 53/157 (33.76%), Postives = 77/157 (49.04%), Query Frame = 1 
Query:  421 KGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQR--ITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            + C+++V  T+ Y       + GC+S +G   ++GG QEL+L   GC+YS G I+HE  HA GF H Q R+DRD +V I W+ I     + F           Y  +S+MHY  ++F     R  IT +   N  I   +G    D      LY C+
Sbjct:  127 RTCIRFVRRTNEYDFISVVSKNGCYSELG---RKGGQQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRSNVLYNCR 279
[Close]
BLAST of Ast5 protein vs. UniProt/SwissProt
Match: sp|P07584|ASTA_ASTAS (Astacin OS=Astacus astacus OX=6715 PE=1 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 7.488e-16
Identity = 55/176 (31.25%), Postives = 89/176 (50.57%), Query Frame = 1 
Query:  370 TKKQIVRESMNELTNDLKGCVKYV-ESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAF-VTCKNCEETVTYKTNSVMHYPSESFPCP-GQRITMVDKQNK---EIPYNHG-TIEEDHQMIKHLY 876
              +  +   M EL    K C+++V  +T   ++++ +   GCWS+VG ++   G+Q+++L+  GC+Y  GTI HE +HA GF H   R DRD +V I ++ +D      F +   +      Y+  S+MHY   SF    G   T+V  QN      PY+    ++ D   I +LY
Sbjct:   74 ADQSAILSGMQELEE--KTCIRFVPRTTESDYVEIFTSGSGCWSYVGRIS---GAQQVSLQANGCVY-HGTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNGIDLTDPYDKAHMLQTDANQINNLY 243
[Close]
BLAST of Ast5 protein vs. UniProt/SwissProt
Match: sp|P0DJJ2|ASTL_CHICK (Astacin-like metalloendopeptidase OS=Gallus gallus OX=9031 GN=ASTL PE=2 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 8.111e-16
Identity = 54/169 (31.95%), Postives = 79/169 (46.75%), Query Frame = 1 
Query:  385 VRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEET-VTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            +RE+M E   +   C+ +V+  +     +     GCWS  G   K GG Q +++   GC++ KG I+HE  HA GF+H   R+DRDK+V I WE I       F   +N     + Y  +SVMHY   +F     + T+V   +  +      G    D   I  LY C
Sbjct:  122 IREAMAEF--ETLTCINFVKRKTERDYLIIRSADGCWSNYG---KVGGGQTISVMKGGCMW-KGIIQHELDHALGFLHEHSRSDRDKYVRIMWEYISPADRPDFKKFENSNNLGLPYDYSSVMHYGPHTFTNTTGKATIVPVPDGSVHIGQRLGLSNLDVAKINRLYNC 284
[Close]
BLAST of Ast5 protein vs. UniProt/SwissProt
Match: sp|P42664|UVS2_XENLA (Embryonic protein UVS.2 OS=Xenopus laevis OX=8355 PE=2 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 5.599e-15
Identity = 54/154 (35.06%), Postives = 72/154 (46.75%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            CV++V  T+  +        GC S +G   K GG+Q L L+  GC+ + G I+HE  HA GF H Q R+DRD +V I  E I  DF   F         + Y   SVMHY    +   G  IT+  K +  +P     G    D   I  LY+C
Sbjct:  137 CVRFVPWTTEVNFLNIMSNGGCGSLIG---KNGGAQRLELDANGCM-NMGIIQHELNHALGFYHEQNRSDRDDYVIIHTENIIPDFLKMFEKYNTNNLGIEYDYASVMHYSRYHYSINGD-ITIEPKPDPNVPIGQRDGLSILDISKINKLYEC 285
[Close]
BLAST of Ast5 protein vs. TrEMBL
Match: A0A060Q750 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast5 PE=2 SV=1)

HSP 1 Score: 365.155 bits (936), Expect = 1.445e-143
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK
Sbjct:   82 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 252

HSP 2 Score: 174.481 bits (441), Expect = 1.445e-143
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 3 
Query:  129 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 377
            MNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK
Sbjct:    1 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 83
[Close]
BLAST of Ast5 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 9.697e-54
Identity = 91/173 (52.60%), Postives = 110/173 (63.58%), Query Frame = 1 
Query:  367 QTKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            Q K Q VR+S+ EL  DL+ CVK+ E  +G++I+V S + GC+S +G+    G SQ LNLENPGC+YSKGTIKHEFIHA GFMHT MR DRD  + I W+RI +  C  F  C  CE    Y+T SVMHY SE F C      +V K    I YNH T   D QMIK+ Y CK
Sbjct:   81 QDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTG--GPSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNCK 251

HSP 2 Score: 58.5362 bits (140), Expect = 9.697e-54
Identity = 30/80 (37.50%), Postives = 48/80 (60.00%), Query Frame = 3 
Query:  153 FLLSILNLVFIE--AGKCGCQKSKLK---RAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADK 374
             +L +L  V I+   G+CGC +  +K   R + ++K+ DMLLD  D+  I GF+ +  N+++W +N + YTF   FP DK
Sbjct:    4 LILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDK 83
[Close]
BLAST of Ast5 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.903e-53
Identity = 86/171 (50.29%), Postives = 109/171 (63.74%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPC-PGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            KK IVRE + EL  DL  CVK+ EST+ ++I+V S   GC+S +G     G +Q LNL+NPGC+YSKGT+KHEFIHA GFMHT MR DRD  + I W+RI +  C  FV C+ C+    Y+TNSVMHYPS  F C PG+ +    +    I YNH T   D  M++  Y C
Sbjct:   81 KKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTG--GPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVF-KRDGGLIDYNHVTSRNDLDMVRKFYAC 248

HSP 2 Score: 65.855 bits (159), Expect = 3.903e-53
Identity = 32/76 (42.11%), Postives = 49/76 (64.47%), Query Frame = 3 
Query:  156 LLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 377
            LL I+  V   +G+CGC + KL KR + S+++ DM+LD  D+  I GF+ +  N +RW +N + ++F P FP DKK
Sbjct:    7 LLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKK 82
[Close]
BLAST of Ast5 protein vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 7.094e-52
Identity = 83/172 (48.26%), Postives = 105/172 (61.05%), Query Frame = 1 
Query:  367 QTKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            + K  +VR+ + EL  DL  CVK+ ES  G++I+V S Q GC+S +G       +Q LNLENPGC+YSKGT+KHEFIHA GFMHT MR DRD  + I W RI++  C  F  C  CE    Y+T SVMHY SE F C  +   ++ K    I YNH T   D  MI++ Y C
Sbjct:   79 RDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMP-TQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNMIRNFYGC 249

HSP 2 Score: 63.5438 bits (153), Expect = 7.094e-52
Identity = 31/84 (36.90%), Postives = 53/84 (63.10%), Query Frame = 3 
Query:  129 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADK 374
            M ++F+    ++++  V   +G+CGC + KL KR++ S+K+ DMLLD  D+  I GF+ +  N ++W +N + Y+F   FP DK
Sbjct:    1 MQKMFLMCLAIAVIGYV---SGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDK 81
[Close]
BLAST of Ast5 protein vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 8.239e-51
Identity = 84/171 (49.12%), Postives = 108/171 (63.16%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            K+ +V++ +NEL +DL  CV++ EST G HI V +   GC++ +G   ++G  Q LNL+NP C+ SKGTIKHEFIHA GFMHT MR DRD F++I W  I S  C  FV C+ C     Y+T SVMHY S++F C     TM+ K    I YNH T   D  MIK LY C+
Sbjct:   84 KRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIGCTGERG--QFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSMIKSLYNCR 252

HSP 2 Score: 56.9954 bits (136), Expect = 8.239e-51
Identity = 33/84 (39.29%), Postives = 46/84 (54.76%), Query Frame = 3 
Query:  135 QIFIGLFLLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKKT 380
            Q F+ L +L + N V   A +CGC K KL  RA  +L + DMLL   D+  I GF+ + + AR W    + + F   FPADK+ 
Sbjct:    6 QKFLILIVLVLANEV---ASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRN 86
[Close]
BLAST of Ast5 protein vs. Ensembl Cavefish
Match: hce2l1 (high choriolytic enzyme 1-like [Source:NCBI gene;Acc:103038207])

HSP 1 Score: 98.2117 bits (243), Expect = 1.095e-23
Identity = 60/164 (36.59%), Postives = 82/164 (50.00%), Query Frame = 1 
Query:  397 MNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            M  L    K CVK+V  T   +      + GCWS++GM+   GG+Q L+L+ PGC++S G   HE +HA GF+H Q R+DRD++V I W+ I     H F           Y  +SVMHY   +F   G   T++ K +  IP     G    D   I  LY C
Sbjct:  118 MGMLDISSKSCVKFVPRTHQANFLDIQPKFGCWSYLGMV---GGTQALSLQTPGCMWS-GVASHELMHALGFVHEQSRSDRDRYVTILWDNIIEGQKHNFRMYDTNNLNTAYDYSSVMHYGRFAFSEEGLP-TIIPKPDPNIPIGQREGPSPLDIHKINLLYNC 276
[Close]
BLAST of Ast5 protein vs. Ensembl Cavefish
Match: hce2l2 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103038533])

HSP 1 Score: 97.8265 bits (242), Expect = 2.343e-23
Identity = 61/158 (38.61%), Postives = 82/158 (51.90%), Query Frame = 1 
Query:  421 KGCVKYVESTSGY-HIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQ--NKEIPYNHGTIEEDHQMIKHLYQCK 885
            K CV+YV  T    H+ +Q  + GCWS+VG     GG Q L+L+ P C+ S   + HE +HA GF+H Q RADRD +V I W  I  D    F   K     + Y   S+MH+   ++   G   T+V KQ  N ++  + G  E D   I  LYQCK
Sbjct:  143 KTCVRYVPRTHQRDHLDIQP-RSGCWSYVG---ANGGRQVLSLQTPECIGS-AVVAHELMHALGFLHEQSRADRDNYVTILWSNIWKDRTRNFAKFKTNNMDMPYDYKSIMHFGKYAYSEDGN-PTIVQKQNWNGKLGQSFGPSELDILKINKLYQCK 294
[Close]
BLAST of Ast5 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 90.5077 bits (223), Expect = 7.534e-21
Identity = 62/143 (43.36%), Postives = 78/143 (54.55%), Query Frame = 1 
Query:  460 HIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEE----DHQMIKHLY 876
            +I VQS + GC+SFVG   + GG Q L+L++ GCLY K  I+HEF+HA GF H Q R+DRDK + I +E ID    H F           Y   SVMHY  ++F   GQ  TMV   N+ +    G  EE    D   IK LY
Sbjct:  131 YIDVQSLE-GCYSFVG---RTGGRQTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQP-TMVPIPNQNVAV--GRAEEMSANDILRIKKLY 265
[Close]
BLAST of Ast5 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 90.1225 bits (222), Expect = 9.309e-21
Identity = 62/143 (43.36%), Postives = 78/143 (54.55%), Query Frame = 1 
Query:  460 HIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEE----DHQMIKHLY 876
            +I VQS + GC+SFVG   + GG Q L+L++ GCLY K  I+HEF+HA GF H Q R+DRDK + I +E ID    H F           Y   SVMHY  ++F   GQ  TMV   N+ +    G  EE    D   IK LY
Sbjct:  142 YIDVQSLE-GCYSFVG---RTGGRQTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQP-TMVPIPNQNVAV--GRAEEMSANDILRIKKLY 276
[Close]
BLAST of Ast5 protein vs. Ensembl Cavefish
Match: ENSAMXT00000021359.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000034.1:1219778:1226321:-1 gene:ENSAMXG00000020723.2 transcript:ENSAMXT00000021359.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.7373 bits (221), Expect = 1.110e-20
Identity = 50/170 (29.41%), Postives = 86/170 (50.59%), Query Frame = 1 
Query:  379 QIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGT--IEEDHQMIKHLYQC 882
            Q+++  ++  ++    C+++ + ++         + GC+S+VG   ++G  Q ++L+  GC+Y   T++HE +HA GF H Q R+DRD  + + WE I   + +AF       +   Y  NSVM Y   +F   GQ  TMV   N  + +  GT   + D   +  LY C
Sbjct:  101 QVIQRGLDSFSS--VSCIRFTQRSNQRDYISIEARSGCYSYVG---RRGNGQTVSLDRNGCIY-HNTVQHELLHALGFNHEQCRSDRDNHIRVVWENIVDSYKYAFDKINTLNQGTPYDYNSVMQYHRTAFSKNGQ-ATMVPIPNANVSFGQGTQMSQNDIARLNRLYGC 263
[Close]
BLAST of Ast5 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.9954 bits (136), Expect = 2.660e-10
Identity = 34/90 (37.78%), Postives = 45/90 (50.00%), Query Frame = 1 
Query:  490 CWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERI-DSDFCHAFVTCKNCEETVTYKTNSVMHY 756
            CWSFVGM+    G Q +++   GC    GT+ HE  HA G++H Q R DRD F+++    + D       V        V Y   SVMHY
Sbjct:    1 CWSFVGMIGV--GEQVVSIGR-GC-EGVGTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHY 86
[Close]
BLAST of Ast5 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 49.2914 bits (116), Expect = 6.747e-7
Identity = 39/138 (28.26%), Postives = 63/138 (45.65%), Query Frame = 1 
Query:  370 TKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQP-GCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETV---TYKTNSVMHYPSESF 771
            ++K + R++M         CV ++E T      V +++P GC S+VG   + GG Q +++      +  G + HE  H  GF H   R DRD  V I    I     + F+  +  E      +Y  +S+MHY   +F
Sbjct:   38 SQKAMFRQAMRHWEKHT--CVTFIERTDEESYIVFTYRPCGCCSYVG--RRGGGPQAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTF 169
[Close]
BLAST of Ast5 protein vs. Ensembl Nematostella
Match: EDO39444 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SA80])

HSP 1 Score: 83.9593 bits (206), Expect = 1.094e-19
Identity = 57/158 (36.08%), Postives = 79/158 (50.00%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVT-----YKTNSVMHYPSESFPCPGQ-RITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            C+K+V  T   +  V  ++ GC+S VGML  + G Q+L+L   GC YS GTI HE +HA+GF H Q R DR+++V + WE ID    H F   K   E++      Y  +S+MHY    F    +  +  V   NK      G    D   +  LY C
Sbjct:   10 CLKWVPKTDEKNWVVIVNKGGCYSSVGMLYWREGGQDLSL-GSGC-YSVGTIMHEMLHASGFWHEQSRTDRNQYVEVMWENIDDGKAHNF--NKYYHESLDMLGKPYDYDSIMHYGKYGFSKNNKPTLQAVGDPNKNFGQRSGFSANDILELNILYDC 163
[Close]
BLAST of Ast5 protein vs. Ensembl Nematostella
Match: EDO36339 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ13])

HSP 1 Score: 87.0409 bits (214), Expect = 1.968e-19
Identity = 57/158 (36.08%), Postives = 77/158 (48.73%), Query Frame = 1 
Query:  421 KGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCE---ETVTYKTNSVMHYPSESFPCPGQ-RITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            + C+K+V  TS           GCWS+VG   +QGG+Q L+L + GC + +GT+ HEF HA GF H Q R DRD +V I W  I  +  H F      E     V+Y   S+MHY   +F   GQ  I   D    ++    G    D +    LY+C
Sbjct:   83 RSCLKFVRRTSEKDYINIFQGSGCWSYVG---RQGGAQNLSLAS-GC-WQRGTVAHEFGHAMGFWHEQSRPDRDDYVTIVWANIPEEKKHNFHMYSRLEIDSLGVSYDYLSIMHYSPTAFSSNGQPTIIPKDPNAVQLGQRVGFSPSDEKQADLLYKC 235
[Close]
BLAST of Ast5 protein vs. Ensembl Nematostella
Match: EDO30117 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1P7])

HSP 1 Score: 80.4925 bits (197), Expect = 1.586e-18
Identity = 54/156 (34.62%), Postives = 76/156 (48.72%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNC---EETVTYKTNSVMHYPSESFPCPGQR-ITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            C+++V  T+  +      + GCWS VG      G Q+L+L + GC ++KGTI HE +HA+GF H Q R DR+ +V + WE I     H F         +    Y  +S+MHY S SF   G+R I  V    K +    G    D   +  LY C
Sbjct:   15 CIRWVPRTTEANWVKIVKETGCWSSVGKSYWSAGFQQLSLGD-GC-HNKGTIMHEMMHASGFWHEQSRPDRNNYVEVMWENIAEGKSHNFNKYDRGQIDDLNANYDFSSLMHYGSFSFSKNGKRTIQAVGDPTKGLGQRDGFSVNDIFELNALYDC 168
[Close]
BLAST of Ast5 protein vs. Ensembl Nematostella
Match: EDO40419 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S725])

HSP 1 Score: 75.8702 bits (185), Expect = 7.706e-17
Identity = 54/175 (30.86%), Postives = 90/175 (51.43%), Query Frame = 1 
Query:  370 TKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAF-VTCKNCEETVT--YKTNSVMHYPSESFPCPGQR-ITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            + ++ +R+++ E     K CV++V  ++       S   GC+S +G   +QGG Q+L++   GC Y +GT+ HE +HA GF H   R DRDK+V I W  I+  F + F V   +  +T+   Y  +S+MHY  ++F    Q  I  +D   +++       E D   +  +Y C
Sbjct:    1 SARKAIRKAIREYHK--KTCVRFVTRSTQPDFVHFSLDYGCYSPIG---RQGGRQKLSV-GEGCEY-RGTVMHELMHALGFFHEHSRHDRDKYVKILWWNIEPGFFNNFNVYSHSVLDTLNAPYDYHSLMHYDKQAFSKNRQNTIEALDNPTRKLGQMDDFSETDINQLLKVYPC 168
[Close]
BLAST of Ast5 protein vs. Ensembl Nematostella
Match: EDO37770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEU5])

HSP 1 Score: 77.0258 bits (188), Expect = 3.308e-16
Identity = 53/171 (30.99%), Postives = 83/171 (48.54%), Query Frame = 1 
Query:  382 IVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKT----NSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            +++ +M E  +  K CV++ + T+  +        GCWS+VGML++  G+Q ++L   GC + KGT  HE  HA GF H Q R DRD +V + W+ I +D  H F    + +   T+ T     S+MHY +  F   G+      K   +I       + D   +   Y C
Sbjct:   85 VIQNAMREWES--KTCVRFQKRTTQKNYVYFFKGDGCWSYVGMLDR--GAQAISL-GSGC-WHKGTAAHEIGHALGFYHEQSRPDRDNYVTVHWDNIKTDSKHNFNK-YSADRINTFGTPYDFGSLMHYGTNYFSVNGRATITPKKSGAKIGQRDYLSDLDVHQMNLRYGC 248
[Close]
BLAST of Ast5 protein vs. Ensembl Medaka
Match: mc6ast5 (astacin like metallo-protease [Source:NCBI gene;Acc:100049509])

HSP 1 Score: 100.908 bits (250), Expect = 1.379e-24
Identity = 59/154 (38.31%), Postives = 79/154 (51.30%), Query Frame = 1 
Query:  427 CVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNH--GTIEEDHQMIKHLYQC 882
            C+K+V  T   H      + GCWSF+G   + GGSQ L+L+ PGC++S G   HEF+HA GF+H Q R+DRD +V I W+ I  D  H F           Y  +SVMHY   +F   G   T++ + +  IP     G    D   I  LY C
Sbjct:  131 CIKFVPRTHEAHFLDIQPRYGCWSFLG---QTGGSQTLSLQTPGCMWS-GIAAHEFMHALGFVHEQSRSDRDHYVTIVWKNIMPDHAHNFRKQATNNLNSPYDYSSVMHYGRYAFSGDGGP-TIIPRPDPYIPIGQRDGPSALDLHKINVLYDC 279
[Close]
BLAST of Ast5 protein vs. Ensembl Medaka
Match: mc6ast4 (astacin like metallo-protease [Source:NCBI gene;Acc:100049317])

HSP 1 Score: 92.4337 bits (228), Expect = 1.501e-21
Identity = 54/155 (34.84%), Postives = 80/155 (51.61%), Query Frame = 1 
Query:  427 CVKYVESTSGY-HIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGT--IEEDHQMIKHLYQC 882
            CV++V  T    ++ +Q  + GCWS++G    +GG Q L+L+NP C+   G I HEF+HA GF+H Q R DRD ++ + W  I  D    F   K     + Y   S+MH+   ++   GQ  T+V K N+ +     +     D   I  LYQC
Sbjct:  127 CVRFVPQTHQRDYVDIQP-KSGCWSYLG---ARGGRQTLSLQNPDCM-QVGVISHEFMHALGFVHEQSRFDRDNYITVMWPNIWRDRLRNFEKFKTDSLDLPYDYGSIMHFGMYAYSMDGQP-TLVAKNNRNMKLGQASSLSHTDKLKINRLYQC 275
[Close]
BLAST of Ast5 protein vs. Ensembl Medaka
Match: ENSORLT00000018637.2 (pep primary_assembly:ASM223467v1:3:32702055:32705063:-1 gene:ENSORLG00000014863.2 transcript:ENSORLT00000018637.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.6633 bits (226), Expect = 2.218e-21
Identity = 53/157 (33.76%), Postives = 78/157 (49.68%), Query Frame = 1 
Query:  421 KGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYN--HGTIEEDHQMIKHLYQCK 885
            + C+++V  T+ Y       + GC+S +G   ++GG QEL+L   GC+YS G I+HE  HA GF H Q R+DRD +V I W+ I     + F           Y  +S+MHY  ++F     R T+    N  +P    +G    D   I  LY C+
Sbjct:  118 RTCIRFVRRTNEYDFISVVSKNGCYSELG---RKGGQQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDTITPIPNPNVPIGQRNGMSRWDITRINVLYNCR 270
[Close]
BLAST of Ast5 protein vs. Ensembl Medaka
Match: hce (Oryzias latipes protease (hce), mRNA. [Source:RefSeq mRNA;Acc:NM_001201498])

HSP 1 Score: 91.6633 bits (226), Expect = 2.881e-21
Identity = 55/157 (35.03%), Postives = 78/157 (49.68%), Query Frame = 1 
Query:  421 KGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQR--ITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            K C+++V  T+ Y       + GC+S +G   ++GG QEL++   GC+YS G I+HE  HA GF H Q R+DRD +V I WE I     + F           Y  +S+MHY  ++F     R  IT +   N  I   +G    D   I  LY C+
Sbjct:  127 KTCIRFVRRTNEYDFISVVSKTGCYSELG---RKGGLQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDITRINVLYNCR 279
[Close]
BLAST of Ast5 protein vs. Ensembl Medaka
Match: ENSORLT00000018649.2 (pep primary_assembly:ASM223467v1:3:32704054:32707947:-1 gene:ENSORLG00000014873.2 transcript:ENSORLT00000018649.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 88.1965 bits (217), Expect = 7.707e-20
Identity = 49/141 (34.75%), Postives = 73/141 (51.77%), Query Frame = 1 
Query:  421 KGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTI 843
            + C+++V  T+ Y       + GC+S +G   ++GG QEL+L   GC+YS G I+HE  HA GF H Q R+DRD +V I W+ I     + F           Y  +S+MHY  ++F     R    D  N   PY++ +I
Sbjct:  127 RTCIRFVRRTNEYDFISVVSKNGCYSELG---RKGGQQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR----DTNNLNTPYDYSSI 259
[Close]
BLAST of Ast5 protein vs. Planmine SMEST
Match: SMESG000038837.1 (SMESG000038837.1)

HSP 1 Score: 351.673 bits (901), Expect = 1.514e-148
Identity = 170/187 (90.91%), Postives = 170/187 (90.91%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD----------------FCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD                 CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK
Sbjct:   92 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDCKFFLWFSKIPEFFYIVCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 278

HSP 2 Score: 194.126 bits (492), Expect = 1.514e-148
Identity = 92/93 (98.92%), Postives = 92/93 (98.92%), Query Frame = 3 
Query:   99 MPMSNRVNHKMNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 377
            MPMSNRVNHKMNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKY FHPQFPADKK
Sbjct:    1 MPMSNRVNHKMNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYAFHPQFPADKK 93
[Close]
BLAST of Ast5 protein vs. Planmine SMEST
Match: SMESG000038837.1 (SMESG000038837.1)

HSP 1 Score: 351.673 bits (901), Expect = 4.294e-147
Identity = 170/187 (90.91%), Postives = 170/187 (90.91%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD----------------FCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD                 CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK
Sbjct:   90 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDCKFFLWFSKIPEFFYIVCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 276

HSP 2 Score: 189.119 bits (479), Expect = 4.294e-147
Identity = 90/91 (98.90%), Postives = 90/91 (98.90%), Query Frame = 3 
Query:  105 MSNRVNHKMNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 377
            MSNRVNHKMNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKY FHPQFPADKK
Sbjct:    1 MSNRVNHKMNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYAFHPQFPADKK 91
[Close]
BLAST of Ast5 protein vs. Planmine SMEST
Match: SMESG000038837.1 (SMESG000038837.1)

HSP 1 Score: 351.288 bits (900), Expect = 6.361e-142
Identity = 170/187 (90.91%), Postives = 170/187 (90.91%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD----------------FCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD                 CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK
Sbjct:   82 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDCKFFLWFSKIPEFFYIVCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 268

HSP 2 Score: 172.17 bits (435), Expect = 6.361e-142
Identity = 82/83 (98.80%), Postives = 82/83 (98.80%), Query Frame = 3 
Query:  129 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 377
            MNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKY FHPQFPADKK
Sbjct:    1 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKLKRAISLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYAFHPQFPADKK 83
[Close]
BLAST of Ast5 protein vs. Planmine SMEST
Match: SMESG000038838.1 (SMESG000038838.1)

HSP 1 Score: 334.339 bits (856), Expect = 4.773e-117
Identity = 162/179 (90.50%), Postives = 162/179 (90.50%), Query Frame = 1 
Query:  397 MNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD----------------FCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 885
            MNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSD                 CHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK
Sbjct:    1 MNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDCKFFLWFSKIPEFFYIVCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQCK 179
[Close]
BLAST of Ast5 protein vs. Planmine SMEST
Match: SMESG000072791.1 (SMESG000072791.1)

HSP 1 Score: 172.17 bits (435), Expect = 2.214e-56
Identity = 86/171 (50.29%), Postives = 109/171 (63.74%), Query Frame = 1 
Query:  373 KKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPC-PGQRITMVDKQNKEIPYNHGTIEEDHQMIKHLYQC 882
            KK IVRE + EL  DL  CVK+ EST+ ++I+V S   GC+S +G     G +Q LNL+NPGC+YSKGT+KHEFIHA GFMHT MR DRD  + I W+RI +  C  FV C+ C+    Y+TNSVMHYPS  F C PG+ +    +    I YNH T   D  M++  Y C
Sbjct:   81 KKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTG--GPNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVF-KRDGGLIDYNHVTSRNDLDMVRKFYAC 248

HSP 2 Score: 65.855 bits (159), Expect = 2.214e-56
Identity = 32/76 (42.11%), Postives = 49/76 (64.47%), Query Frame = 3 
Query:  156 LLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKK 377
            LL I+  V   +G+CGC + KL KR + S+++ DM+LD  D+  I GF+ +  N +RW +N + ++F P FP DKK
Sbjct:    7 LLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKK 82
The following BLAST results are available for this feature:
BLAST of Ast5 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MEP1A8.549e-1441.46meprin A subunit alpha [Source:HGNC Symbol;Acc:HGN... [more]
MEP1A9.003e-1441.46meprin A subunit alpha [Source:HGNC Symbol;Acc:HGN... [more]
MEP1B1.357e-1343.88meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC... [more]
MEP1B1.395e-1343.88meprin A subunit beta [Source:HGNC Symbol;Acc:HGNC... [more]
ASTL1.127e-1129.77astacin like metalloendopeptidase [Source:HGNC Sym... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-131.088e-1437.04Zinc metalloproteinase nas-13 [Source:UniProtKB/S... [more]
nas-41.456e-1431.07Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-43.280e-1431.07Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-43.493e-1431.07Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-158.199e-1432.18Zinc metalloproteinase nas-15 [Source:UniProtKB/S... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG67633.799e-2334.18gene:FBgn0039069 transcript:FBtr0084378[more]
CG67633.799e-2334.18gene:FBgn0039069 transcript:FBtr0334497[more]
CG57157.018e-1933.33gene:FBgn0039180 transcript:FBtr0084634[more]
CG152532.573e-1836.36gene:FBgn0028948 transcript:FBtr0080782[more]
CG102802.192e-1644.33gene:FBgn0037395 transcript:FBtr0309318[more]
back to top
BLAST of Ast5 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l12.110e-2337.66high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
hce2l14.767e-2337.66high choriolytic enzyme 2-like 1 [Source:ZFIN;Acc:... [more]
hce2l22.178e-2034.52high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
hce2l21.088e-1934.34high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
he1.21.164e-1933.55hatching enzyme 1, tandem dupliate 2 [Source:ZFIN;... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000022029.13.439e-2132.76astacin-like metalloendopeptidase [Source:NCBI gen... [more]
ENSXETT00000006778.14.692e-2135.48astacin-like metalloendopeptidase [Source:NCBI gen... [more]
ENSXETT00000006820.14.901e-2135.48astacin-like metalloendopeptidase [Source:NCBI gen... [more]
kpna46.666e-2037.01karyopherin alpha 4 (importin alpha 3) [Source:Xen... [more]
golt1b1.097e-1932.95golgi transport 1B [Source:Xenbase;Acc:XB-GENE-575... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mep1a1.317e-1435.67meprin 1 alpha [Source:MGI Symbol;Acc:MGI:96963][more]
Mep1a1.518e-1435.67meprin 1 alpha [Source:MGI Symbol;Acc:MGI:96963][more]
Astl1.497e-1237.62astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl1.781e-1237.62astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl1.781e-1237.62astacin-like metalloendopeptidase (M12 family) [So... [more]
back to top
BLAST of Ast5 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P31580|HCE1_ORYLA1.833e-2035.03High choriolytic enzyme 1 OS=Oryzias latipes OX=80... [more]
sp|P31581|HCE2_ORYLA4.866e-1933.76High choriolytic enzyme 2 OS=Oryzias latipes OX=80... [more]
sp|P07584|ASTA_ASTAS7.488e-1631.25Astacin OS=Astacus astacus OX=6715 PE=1 SV=2[more]
sp|P0DJJ2|ASTL_CHICK8.111e-1631.95Astacin-like metalloendopeptidase OS=Gallus gallus... [more]
sp|P42664|UVS2_XENLA5.599e-1535.06Embryonic protein UVS.2 OS=Xenopus laevis OX=8355 ... [more]
back to top
BLAST of Ast5 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A060Q7501.445e-143100.00Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7149.697e-5452.60Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7863.903e-5350.29Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7857.094e-5248.26Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
I1ZIC38.239e-5149.12Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l11.095e-2336.59high choriolytic enzyme 1-like [Source:NCBI gene;A... [more]
hce2l22.343e-2338.61low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast17.534e-2143.36low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast19.309e-2143.36low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000021359.21.110e-2029.41pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000004913.12.660e-1037.78pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
ENSPMAT00000007253.16.747e-728.26pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Ast5 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO394441.094e-1936.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO363391.968e-1936.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO301171.586e-1834.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO404197.706e-1730.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377703.308e-1630.99Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Ast5 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mc6ast51.379e-2438.31astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast41.501e-2134.84astacin like metallo-protease [Source:NCBI gene;Ac... [more]
ENSORLT00000018637.22.218e-2133.76pep primary_assembly:ASM223467v1:3:32702055:327050... [more]
hce2.881e-2135.03Oryzias latipes protease (hce), mRNA. [Source:RefS... [more]
ENSORLT00000018649.27.707e-2034.75pep primary_assembly:ASM223467v1:3:32704054:327079... [more]
back to top
BLAST of Ast5 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038837.11.514e-14890.91SMESG000038837.1[more]
SMESG000038837.14.294e-14790.91SMESG000038837.1[more]
SMESG000038837.16.361e-14290.91SMESG000038837.1[more]
SMESG000038838.14.773e-11790.50SMESG000038838.1[more]
SMESG000072791.12.214e-5650.29SMESG000072791.1[more]
back to top
Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30014093
GenBankCCW36843.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014093 ID=SMED30014093|Name=Ast5 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=889bp
TTTTTTCATATAATAAAAATTTAATTGAATAAATATTTTTCTTGAATATT
TCCGAATTTCTTTAAAATAACATTTTGATCAGTATAAAAACTCGATTTAT
GCCAATGTCAAATCGGGTAAACCATAAAATGAATCAAATATTTATTGGAC
TGTTTCTGTTGAGCATTCTCAATCTTGTTTTCATTGAAGCTGGAAAATGC
GGCTGCCAAAAATCGAAATTGAAAAGAGCAATTAGTTTGAAAATCGATGA
CATGTTGCTTGACCATGTTGATCTGATGAAGATAGAAGGGTTCCGAGGCT
ATGATAAAAATGCCAGGAGATGGACTGACAATACTCTAAAATATACTTTC
CATCCTCAATTTCCTGCAGACAAAAAAACAAATAGTTAGAGAGTCAATGA
ATGAATTGACAAATGACTTGAAAGGATGTGTAAAATATGTTGAGAGTACC
TCTGGATATCACATTAAAGTACAGTCTCATCAACCAGGTTGTTGGTCATT
TGTGGGAATGTTGAACAAACAAGGAGGGTCTCAAGAATTAAATTTAGAAA
ATCCAGGATGTCTGTATTCGAAAGGTACAATAAAGCACGAATTCATCCAT
GCAACGGGATTCATGCATACACAAATGAGAGCTGACAGGGATAAATTTGT
GAATATTTTCTGGGAAAGGATTGATTCAGACTTTTGCCATGCTTTTGTAA
CTTGCAAGAACTGTGAAGAAACAGTGACATACAAGACGAATAGTGTAATG
CATTATCCGAGTGAGTCGTTTCCATGTCCTGGGCAAAGAATAACAATGGT
TGATAAACAAAATAAAGAGATTCCCTACAATCACGGAACGATTGAAGAAG
ACCACCAAATGATTAAGCATTTATATCAATGCAAATAAG
back to top

protein sequence of SMED30014093-orf-1

>SMED30014093-orf-1 ID=SMED30014093-orf-1|Name=SMED30014093-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
MNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNL
ENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAF
VTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIE
EDHQMIKHLYQCK*
back to top
Aliases
The feature 'Ast5 protein' has the following synonyms
Synonym
Ast5
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
GO:0008233peptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..31
score: 0.704
NoneNo IPR availableTMHMMTMhelixcoord: 15..32