Transposable element Tcb1 transposase

Overview
NameTransposable element Tcb1 transposase
Smed IDSMED30014069
Length (bp)3291
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transposable element Tcb1 transposase (SMED30014069) t-SNE clustered cells

Violin plots show distribution of expression levels for Transposable element Tcb1 transposase (SMED30014069) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014069

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30014069SMESG000010591.1 dd_Smed_v4_10661_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30014069SMESG000038869.1 OX_Smed_1.0.03638ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Category 4 cellSMED30014069SMESG000010591.1 dd_Smed_v4_10661_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transposable element Tcb1 transposase vs. TrEMBL
Match: A0A4C1Y486 (BED-type domain-containing protein OS=Eumeta variegata OX=151549 GN=EVAR_52339_1 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 7.337e-26
Identity = 63/177 (35.59%), Postives = 98/177 (55.37%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKHESAHTLHXXXXXXXXXXXXX-----SCNDEPXXXXXXXXXXXXXQLKLVRKIVCFLRYPPVCNSILQNHVK 2713
            F+++ DEW+SLR RRY NI ++  +    +LGLV I   C A E+   +K+ L +F + FE D+V+ T+DG  +++K  R  P E+++CLNH++HL   + F KK+E+          D  +  +      +C+ EP I  N  +NI    L+  R +V   R  P+ N  LQ HVK
Sbjct:  168 FNLSVDEWSSLRNRRYFNICIHHTDSNFYNLGLVYIPGQCRAVEIKEIIKHRLEEFGLYFEQDVVAVTSDGPNVMVKFGRENPTEMVLCLNHAVHLAVLETFCKKNETNENFTSNQDSDNLSGEESEYDEDNCDLEPEIRTNYTQNIDKA-LQDTRALVKLFRKSPLKNITLQKHVK 343

HSP 2 Score: 51.2174 bits (121), Expect = 7.337e-26
Identity = 31/70 (44.29%), Postives = 41/70 (58.57%), Query Frame = 1 
Query: 1951 TLMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLIQYNFEVKKVEII 2160
            TL      + SL +IV+ LAA D ++IR I  S F RES+  R  +LP  E+ VMK+I   +E KK E I
Sbjct:   85 TLHFEPKNKQSLGEIVAVLAAKDGLTIRQITKSDFIRESLRLRGINLPKNESDVMKIILKYYEEKKQETI 154
[Close]
BLAST of Transposable element Tcb1 transposase vs. TrEMBL
Match: A0A4C1XLJ2 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_59718_1 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 1.607e-22
Identity = 50/105 (47.62%), Postives = 72/105 (68.57%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKK 2512
            FS++ DEWTS+R RRY N+ +Y E+ E  +LGL+ I   C A+E    V++ L +F ++FE  IV+TT+DG  ++ K  R  P E+I+CLNH+IHLG  D FYK+
Sbjct:   15 FSLSVDEWTSVRNRRYFNLCLYVEDGEVFNLGLIYIPGKCGAEETKKMVEDTLKRFQLDFEKHIVATTSDGPNVMKKFGRESPSEMILCLNHAIHLGVVDTFYKE 119
[Close]
BLAST of Transposable element Tcb1 transposase vs. TrEMBL
Match: B0W9I7 (Predicted protein OS=Culex quinquefasciatus OX=7176 GN=6035136 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.282e-21
Identity = 75/268 (27.99%), Postives = 130/268 (48.51%), Query Frame = 2 
Query: 2147 KWKLLKMIENRKMVRDNFSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKHESAHTLHXXXXXXXXXXXXXSCNDEPXXXXXXXXXXXXXQ--LKLVRKIVCFLRYPPVCNSILQNHVKNEFQCEYELLQYIKVLNFVHDR---KDFEAETSISQALKDLNSYYLMKKVDLELMKNVSLSLQPSKLAVEILSRKDA 2935
            K + ++ I+++       S+T DEWTS   R+YLN+NV+  +    +LGLV +  S  A++++  V  HL  F+I  + ++ + T+D A ++ K  +    +  +C NH +HL  CD  +K  E+   L EE   D E+   +  +D       G  N    +  L  +R I    R  PV N+ILQ  V  EF    +L+  +K      ++   +  +    +++ALK+L     +   DLE +  +  +LQP +LA  I  + + 
Sbjct:  141 KQQTVQQIQDKLAELHFLSITLDEWTSSGNRKYLNVNVHFGDGAFFNLGLVRVKGSFPAEKILEAVDQHLECFAIR-DRNVAAATSDAAKVMEKFGKLATFKHQLCFNHGLHLAVCDVIFKSSETPGDLLEESDSDDEDCDSEESDDSKDATIPGLYNSSNYRPVLLQIRNICKLFRRSPVKNAILQKKVVQEFGKPLQLILDVKTRWNSTEQMLARYMKLHGCVAEALKELEDLAPVSTSDLESISQLYDALQPDELAAFINGKAEG 407

HSP 2 Score: 52.7582 bits (125), Expect = 1.282e-21
Identity = 29/85 (34.12%), Postives = 46/85 (54.12%), Query Frame = 1 
Query: 1912 GKRTISD*NSKTSTLMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLIQYNFEVKKVEIIKN 2166
            G +      SK ST+    I +  + ++ ++LAA D ++I  I NS F R S + R Y LP  +T VMKLI   +E+ K + ++ 
Sbjct:   64 GSKQAKSNTSKQSTIE-PFIHKKPMNEMFARLAAQDGLTISAITNSKFIRTSFAQRGYQLPLSKTSVMKLIHDYYEIAKQQTVQQ 147
[Close]
BLAST of Transposable element Tcb1 transposase vs. TrEMBL
Match: A0A0N7Z9Z3 (BED-type domain-containing protein OS=Scylla olivacea OX=85551 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.272e-20
Identity = 43/118 (36.44%), Postives = 65/118 (55.08%), Query Frame = 2 
Query: 2168 IENRKMVRDNFSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKHES 2521
            I  RK V   F ++ DE+TSL+ +RY+NIN++  +    +LG+  I  S  A+  V  V+N L +F +N E D+V+   DGA +++K  +        C  H IHL  CD  YKK + 
Sbjct:  158 INKRKNVGVRFGLSLDEYTSLKNKRYMNINLHTSD-TFWNLGMARITGSVPAETAVEVVENKLAEFQVNLERDVVACVTDGASVMVKFGKLIKTCHHTCYAHGIHLAVCDVLYKKTDG 274

HSP 2 Score: 52.373 bits (124), Expect = 2.272e-20
Identity = 28/69 (40.58%), Postives = 41/69 (59.42%), Query Frame = 1 
Query: 1957 MLNLIKRD-SLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLIQYNFEVKKVEII 2160
            M +L+K++ + E+IV KLAA+D  SI  I  S F + S+ +R Y LP   T VM L+   + V K  +I
Sbjct:   87 MTDLLKKEETKEEIVGKLAAVDGFSINAIAKSEFIKTSMLARGYKLPQSPTLVMDLVHKQYNVAKERVI 155
[Close]
BLAST of Transposable element Tcb1 transposase vs. TrEMBL
Match: A0A0J7KNG8 (Dimer_Tnp_hAT domain-containing protein OS=Lasius niger OX=67767 GN=RF55_8155 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 2.925e-18
Identity = 87/295 (29.49%), Postives = 141/295 (47.80%), Query Frame = 2 
Query: 2147 KWKLLKMIENRKMVRDNFSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYK-----KHESAHTLHXXXXXXXXXXXXXSCNDEPXXXXXXXXXXXXXQLKL----------VRKIVCFLRYPPVCNS-ILQNHVKNEFQCEYELL-----QYIKVLNFVHDRKDFEAETSISQALKDLNSYYLMKKVDLELMKNVSLSLQPSKLAVEILSRKDANVLVPKVFYRF 2968
            K  ++  I+  +M    FS+T DEWTS + RRYLNIN++ +  +  +LGL+ I  S +A+  V  V+ HL  F+IN + DIV+ T DG  +++K     P     C  H I L   D  YK     + +    L      D  +  + S  D+   E +   + I   +K+          VRK+V   R  P  N  ILQ +++ E     EL+     ++  ++N +   + +     IS+AL DL S     + +   + ++  SLQP KL V++L R+DA ++  +   RF
Sbjct:   55 KANMINEIQKLQMQNQKFSLTFDEWTSRKNRRYLNINLHSDK-QHRNLGLIRIQGSSSAEHCVNLVEEHLKIFNINIKDDIVAITADGPNVMIKVGNLMPCFQQRCFAHGIQLAIIDILYKDLPEEEAQDQEGLRRFQEIDNADQSELSDVDDDDNEGSFIVDCIPSSVKIASNYKDLITKVRKVVKIFRRSPTKNDLILQKYIQEEHDKRLELILDCKTRWNSLVNMLE--RFYIVRLCISKALIDLRSEIRFTETEWFAINDLKNSLQPVKLGVKVLCRRDATLITAETTVRF 346
[Close]
BLAST of Transposable element Tcb1 transposase vs. Planmine SMEST
Match: SMESG000004539.1 (SMESG000004539.1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.614e-27
Identity = 45/103 (43.69%), Postives = 69/103 (66.99%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFY 2506
            FS+T DE  S R+ RY+NINV+ +  +  +LGL+ I+ SC   ++   V +HL  F ++FE+D+V+TTNDG  +++K  R  P E+ +CLNH +HL  C+ FY
Sbjct:   97 FSITLDELVSSRQHRYMNINVHDKTGKVFNLGLIRIIGSCTTRKIPSIVTDHLKVFHLSFENDVVATTNDGTSVMVKYGRESPAEVQLCLNHGLHLDVCNKFY 199

HSP 2 Score: 44.2838 bits (103), Expect = 2.614e-27
Identity = 28/85 (32.94%), Postives = 47/85 (55.29%), Query Frame = 1 
Query: 1909 CGKRTISD*NSKTST-----LMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLIQYNFEVKK 2148
            C +   SD  + TST      +++ +K+ +L +IV +LA  D  SIR I  SSF R++I ++ Y LP  E   + +  ++  +KK
Sbjct:    9 CKRTHDSDSKASTSTRSHHKTIVDFMKKITLGEIVDELATKDGFSIRGITRSSFIRQAIHAQGYKLPKNENENIYINYFDEAIKK 93
[Close]
BLAST of Transposable element Tcb1 transposase vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1)

HSP 1 Score: 82.8037 bits (203), Expect = 5.251e-26
Identity = 73/275 (26.55%), Postives = 138/275 (50.18%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKHESAHTLHXXXXXXXXXXXXXSCN-------------DEPXXXXXXXXXXXXXQLKLVRKIVCFLRYPPVCNSILQNHVKNEFQCEYELL-----QYIKVLNFVHDRKDFEAETSISQALKDLNSYYLMKKVDLELMKNVSLSLQPSKLAVEILSRKDANVLVPKVFYRF 2968
            FS+TTDEWTS   +RYLN+ ++ +  +   LGL+ I  SC+A ++   V+  L  + I    +I+++T DGA ++ K ++   +E   C+NH+IHL   + FY K+     + + +  D + D +   +             D      + ++  I E L+  RK V F R  PV N ILQ +V+++   + +L+     ++  ++  +   +    +  I  AL+++    L  + D  ++ N+   L+P++LAV+ LS+  + +L  +    F
Sbjct:  148 FSITTDEWTSCSGKRYLNVTLHSQE-DSYKLGLIAIECSCDAYQIHSLVEQKLMDYGIKLR-NILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGYHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMME--RLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTF 418

HSP 2 Score: 55.8398 bits (133), Expect = 5.251e-26
Identity = 27/62 (43.55%), Postives = 43/62 (69.35%), Query Frame = 1 
Query: 1939 SKTSTLMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLI 2124
            +K ++ MLN IKR+S+E++++K AA D  SI  + +SS  RE I++R Y +P  E+ + KLI
Sbjct:   62 AKATSSMLNFIKRESVEELLAKCAAYDGFSIDAMTHSSAIREFITTRSYVMPRSESTIKKLI 123
[Close]
BLAST of Transposable element Tcb1 transposase vs. Planmine SMEST
Match: SMESG000026901.1 (SMESG000026901.1)

HSP 1 Score: 79.337 bits (194), Expect = 1.774e-24
Identity = 72/281 (25.62%), Postives = 136/281 (48.40%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKH----ESAHTLHXXXXXXXXXXXXXSCN---------DEPXXXXXXXXXXXXXQLKLVRKIVCFLRYPPVCNSILQNHVKNEFQCEYELLQYIKVLNFVHDRKDFEA-----------ETSISQALKDLNSYYLMKKVDLELMKNVSLSLQPSKLAVEILSRKDANVLVPKVFYRF 2968
             S+TTDEWTS   +RYLN+ ++ +  +   LGL+ I  SC+A ++   V+  L  + I    +I+++T DGA ++ K ++   +E   C+NH+IHL   + F+ K+    E + + + +  +D       + +         D      + ++  I E L+  RK V F R  PV N ILQ +V+++   + +L+        +  R  + +           +  I  AL+++    L  + D  ++ N+   L+P++LAV+ LS+  + +L  +    F
Sbjct:  148 LSITTDEWTSCSGKRYLNVTLHSQE-DSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLR-NILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFHNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDAKINEILQNFRKTVRFFRKSPVRNDILQRYVEDKEGKKLKLI--------LDSRTRWNSLIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTF 418

HSP 2 Score: 53.9138 bits (128), Expect = 1.774e-24
Identity = 26/62 (41.94%), Postives = 42/62 (67.74%), Query Frame = 1 
Query: 1939 SKTSTLMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLI 2124
            +K ++ MLN IKR+S+ ++++K AA D  SI  + +SS  RE I++R Y +P  E+ + KLI
Sbjct:   62 AKATSNMLNFIKRESVGELLAKCAAYDGFSIHAMTHSSAIREFITTRSYVMPRSESTIKKLI 123
[Close]
BLAST of Transposable element Tcb1 transposase vs. Planmine SMEST
Match: SMESG000065760.1 (SMESG000065760.1)

HSP 1 Score: 77.411 bits (189), Expect = 1.648e-23
Identity = 39/106 (36.79%), Postives = 65/106 (61.32%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKH 2515
            FS+TTDEWTS   +RYLN+ ++ +  +   LGL+ I  SC+A ++   V+  L  + I    +I+++T DGA ++ K ++   +E   C+NH+IHL   + FY K+
Sbjct:  148 FSITTDEWTSCSGKRYLNVTLHSQE-DSYKLGLIAIEGSCDAYQIHSLVEQKLMDYGIKLR-NILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKN 251

HSP 2 Score: 52.7582 bits (125), Expect = 1.648e-23
Identity = 25/62 (40.32%), Postives = 42/62 (67.74%), Query Frame = 1 
Query: 1939 SKTSTLMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLI 2124
            +K ++ MLN IKR+S+ ++++K +A D  SI  + +SS  RE I++R Y +P  E+ + KLI
Sbjct:   62 AKATSNMLNFIKRESVGELLAKCSAYDGFSIHAMTHSSVIREFITTRSYVMPRSESTIKKLI 123
[Close]
BLAST of Transposable element Tcb1 transposase vs. Planmine SMEST
Match: SMESG000022344.1 (SMESG000022344.1)

HSP 1 Score: 78.5666 bits (192), Expect = 3.711e-23
Identity = 70/275 (25.45%), Postives = 139/275 (50.55%), Query Frame = 2 
Query: 2198 FSVTTDEWTSLRRRRYLNINVYGEN*ECVSLGLVPILRSCNADEMVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSIHLGFCDYFYKKH----ESAHTLHXXXXXXXXXXXXXSCN---------DEPXXXXXXXXXXXXXQLKLVRKIVCFLRYPPVCNSILQNHVKNEFQCEYELL-----QYIKVLNFVHDRKDFEAETSISQALKDLNSYYLMKKVDLELMKNVSLSLQPSKLAVEILSRKDANVLVPKVFYRF 2968
            FS+TTDEWTS   +RYLN+ ++ +  +   LGL+ I  SC+A ++   V+  L  + I    +I+++T DGA ++ K ++   +E   C+NH+IHL   + FY K+    E + + + +  +D       + +         D      + ++  I E L+  RK V F R  PV N ILQ + +++   +++L+     ++  ++  +   +    +  I  AL+++    L  + D  +++N+   L+P++ AV+ LS+  + ++  +    F
Sbjct:  125 FSITTDEWTSCSGKRYLNVTLHSQE-DSYKLGLIAIEGSCDAYQIHSLVEQKLMYYDIKLR-NILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNNVIDEISDSENADGDEDENQFNSFAGDHNLNIDNDIDNTEYTYSLDDANINEILQNFRKTVRFFRKSPVRNDILQRYDEDKEGKKFKLILDCRTRWNSLIPMME--RLVLLQVCIKHALEEIGCDDLYNEKDFNILENLIEILKPTEFAVKELSKHTSTLITAERVLTF 395

HSP 2 Score: 50.447 bits (119), Expect = 3.711e-23
Identity = 31/84 (36.90%), Postives = 49/84 (58.33%), Query Frame = 1 
Query: 1885 KHNFD----EQNCGKRTISD*NSKTSTLMLNLIKRDSLEDIVSKLAAIDNISIRTIMNSSFTRESISSRVYSLPNCETRVMKLI 2124
            KH+ D    E+   KR      +K ++ MLN IKR+S+ ++++K A  D  SI  + +SS  RE I++R Y +P  E+ + KLI
Sbjct:   23 KHDIDVKKNEEPPEKR------AKATSNMLNFIKRESVGELLAKCAVYDGFSIHAMTHSSAIREFITTRSYVMPKSESTIKKLI 100

HSP 3 Score: 52.7582 bits (125), Expect = 1.251e-6
Identity = 23/47 (48.94%), Postives = 36/47 (76.60%), Query Frame = 1 
Query: 3061 KEFSIFKKTIKRTQNIEFLYMALKTASPTSTNSERMFSVSGNFCTKL 3201
            KE  +F  T ++++ ++ L+ AL +  PTST+SER+FS++GNFCTKL
Sbjct:  524 KELKMFGVTKQKSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKL 570
The following BLAST results are available for this feature:
BLAST of Transposable element Tcb1 transposase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4C1Y4867.337e-2635.59BED-type domain-containing protein OS=Eumeta varie... [more]
A0A4C1XLJ21.607e-2247.62Uncharacterized protein OS=Eumeta variegata OX=151... [more]
B0W9I71.282e-2127.99Predicted protein OS=Culex quinquefasciatus OX=717... [more]
A0A0N7Z9Z32.272e-2036.44BED-type domain-containing protein OS=Scylla oliva... [more]
A0A0J7KNG82.925e-1829.49Dimer_Tnp_hAT domain-containing protein OS=Lasius ... [more]
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transposable element Tcb1 transposase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004539.12.614e-2743.69SMESG000004539.1[more]
SMESG000077975.15.251e-2626.55SMESG000077975.1[more]
SMESG000026901.11.774e-2425.62SMESG000026901.1[more]
SMESG000065760.11.648e-2336.79SMESG000065760.1[more]
SMESG000022344.13.711e-2325.45SMESG000022344.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014069 ID=SMED30014069|Name=Transposable element Tcb1 transposase|organism=Schmidtea mediterranea sexual|type=transcript|length=3291bp
AAAACCTTTGGATATTTTTGTCAGGAATTATCGCCAAATATTTGGAGTAC
CGGAAGGTAATCAATTATTATTTTATAACTTTTAAACATTCATTTATTAT
TAAAATACTGTAAATAAGTTATGTAATTTCTATCATTATTTCTAAAGATT
TGCCAACGTTCTTAGAGCTTTATAAATCAATTTTGGAAAAATTTCTATTT
TTCGACCCAACATAAGTTGTTAAGCCACAATTGTCTTAAAAGTAATGACT
GCCTTTGAAAGAGGAAAACGTGATTTTTTCTGACGAATCATAGTTTACCG
TATTTCCTACCTCCAGCCAAGTTTAGGTTTGGAAACAGTCGAAAGAAGCT
TTCAATATAGGCTGCCTTTTGTCAACCGTAAAATATGGCTGGGATTCTAT
GATAATCTGGGGTTCTATTTCTTGGAAATCCAGCGGGAAAATGATTTCTC
TTTATAGAAGAATTAACAGTCGAGACTATTTAGGAAAGTTCATCCTATGG
TCCAAGAACCTTTTCCAGCGGGGAATGCCATTTTTCAAGATGATAATTCC
CCAATCTCATCTGGCCACACAATCCCTAGACCTTAATATTTTTAAGCATT
ATGGTCGATTTTAGAGCTTCAATTCGATTCCCACCTCCATCGTCTCTAAA
TGAACTAGAGGGTGTTTTAACTGAGGAATGGGCTAATATTCATTTGGAAA
CAATTCACAATTTATATGAATCAATATCTTGGAGAATTGAGCCAGTAATA
TTAAATTCAATCGAAATGGTACCAGAAAAACATTCATAGAAAAATTTGAG
GGAAGTGAGGATGATTATATTCTTTTCGTTAGTGAGGAACGCCCGAGGTA
ACAACAAATAACGATAAAAATGAAACCTAAAGCATCAAAAAGTGACGACA
TGAAATTTGACTTACACTCCGACAACGAATATTTATTTTTCGACGAAGAT
CAATAAGAAAACCATGAGGATATAAACAGTCAGAATCAAGACAGTGAGGA
GAGCCAAAGACGAATTGGGATCAGAGTCAGATTTCGAATTAGAAGAGATG
CCAAACTTTATTCTAGATAAAGAGAAAATAAGTATCACCTAAAATGACAT
GTTCCGATATTTCGGAAGATGAATTGAAAGCGGTGACATACTTAACTTGG
CAGTCAAAAAAGGATTAAAAGGATGGTGGCTGTGATGGTAAAAAAACTGT
CAAATCGAGACAGTCCTCTAGGATGAAGGTAATTAGGACATTACTGCGAC
GGTCTCGGGGACAGTCCTATAAATATGTTTGTATTGACAAACAATAACAG
AGAGGGCCTAATAAGTTTTGCGTTAATGTTTACAATGAAAGCAAATAAAG
GTCCAGCATCAAAAACTCCAATTTAACCAAACCCAATCTAATTCAACCAA
ATTCAATCCATCCAATGTGGCTATTAATCTAACAATTTATTCTTTTAGAC
AATTCCTTGTGTTAATGTATATATTAATAATTGATTTTGATTAGGGTTTC
CAAAACGGATAAATCCGTATTCGGTATCCGCGGTTATTTGTACTCTTAAC
CATATTCGATATCCGCGAAAAATCTCACGGTTATCAATTCAAAATTTTTA
AATTAAGTCTGCTAAATTTTCTCAACAAAGTAGGATAGTCATTTTAACAA
AATTTTTAAAACTTTTTAATATTTTACAAATATGTAGCAAACCATGCATT
TTTTGTCATTTGTTTTCAATTTTATGAACATTTATTTAAGAACAAAATAG
AATAGTTAGAAAAAGTTTTGGTAAGTTATATTTTATTTATTGAATTATAA
TATTTTAAAAAGTTGTATTATTCATAGAAAATTTCATTAAAAAGACCAGT
ATCAACAGTTTGGGTTATTTTAAGAAACTGGTAAAAACACAATTTTGATG
AACAAAACTGTGGTAAAAGAACGATATCCGACTAAAATTCAAAGACTTCA
ACTTTAATGTTAAATTTAATTAAGCGAGACAGTTTGGAAGATATAGTGTC
AAAGTTGGCAGCAATTGATAATATTTCAATACGTACTATAATGAATTCAT
CATTTACTCGTGAATCAATTTCTAGTCGGGTATATTCTTTACCAAACTGT
GAAACTAGGGTAATGAAATTAATACAATACAATTTCGAAGTAAAGAAAGT
GGAAATTATTAAAAATGATAGAAAACCGTAAAATGGTTAGAGATAATTTC
AGTGTCACCACTGATGAATGGACAAGTTTAAGAAGACGTAGGTATTTAAA
TATAAATGTCTACGGAGAAAACTAGGAATGTGTGAGTCTGGGGCTAGTTC
CAATCCTAAGGTCTTGTAATGCAGATGAAATGGTTGGTAAAGTTAAAAAT
CACCTGTTTAAATTCAGCATTAATTTCGAAAGTGACATCGTATCTACTAC
CAATGATGGTGCTCCAATGATTCTAAAGGACAGTAGAACTTACCCAGTTG
AACTAATTATATGTTTAAATCACAGTATACACTTAGGTTTTTGTGATTAT
TTTTATAAAAAGCATGAATCTGCTCACACATTGCATGAAGAAGATATTGA
CGATTATGAAAATGATTTTGATGATTCTTGTAATGATGAGCCCGGGATCG
AAAACAATGGAGAGGAAAATATCATTATTGAACAGCTGAAACTAGTCAGG
AAAATTGTGTGCTTTTTAAGATATCCTCCTGTGTGTAATAGTATTCTGCA
AAATCATGTTAAAAATGAATTCCAATGTGAGTATGAACTACTGCAGTACA
TAAAGGTGCTCAATTTCGTCCATGATAGAAAGGATTTTGAAGCTGAAACG
TCAATTTCACAAGCTTTAAAAGATTTGAATTCTTATTATTTGATGAAAAA
AGTTGATTTGGAGCTAATGAAAAATGTCAGTCTTTCTCTACAGCCTTCAA
AACTAGCAGTTGAAATATTAAGTCGAAAAGACGCAAATGTTTTAGTGCCA
AAGGTGTTTTATCGTTTTTGAAAAAAACAAAATGGAAGCACTCAATTCAT
ATATTTCAAATGAATCCAACAATAAACAAGTAGGAATATCAATAACTAAT
AGTACTTATGAAAGAATTTAGCATTTTCAAAAAAACTATTAAAAGAACAC
AAAATATTGAATTTCTCTATATGGCACTAAAAACAGCGAGTCCGACTTCT
ACAAATTCAGAGAGAATGTTTTCAGTTTCTGGAAATTTCTGTACTAAATT
ACATTTCGTCTTTCTTGTGCGCCACTTAATGCATTAGCCTTCTTCCTGAA
ATCCAAATTAAAATAATAAATTAAATTTCAGCTAAATTGTC
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protein sequence of SMED30014069-orf-1

>SMED30014069-orf-1 ID=SMED30014069-orf-1|Name=SMED30014069-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=214bp
MVGKVKNHLFKFSINFESDIVSTTNDGAPMILKDSRTYPVELIICLNHSI
HLGFCDYFYKKHESAHTLHEEDIDDYENDFDDSCNDEPGIENNGEENIII
EQLKLVRKIVCFLRYPPVCNSILQNHVKNEFQCEYELLQYIKVLNFVHDR
KDFEAETSISQALKDLNSYYLMKKVDLELMKNVSLSLQPSKLAVEILSRK
DANVLVPKVFYRF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
GO:0003950NAD+ ADP-ribosyltransferase activity
GO:0003676nucleic acid binding
GO:0004386helicase activity
GO:0005524ATP binding
Vocabulary: biological process
TermDefinition
GO:0006313transposition, DNA-mediated
GO:0015074DNA integration
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR012317Poly(ADP-ribose)pol_cat_dom