SMED30014044

Overview
NameSMED30014044
Smed IDSMED30014044
Length (bp)3689
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30014044 (SMED30014044) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30014044 (SMED30014044) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30014044 (SMED30014044) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30014044 (SMED30014044) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30014044

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30014044SMESG000079607.1 SMESG000049847.1 SMESG000049832.1 SMESG000049756.1 SMESG000040301.1 SMESG000022640.1 SMESG000072592.1 SMESG000049745.1 SMESG000049740.1 SMESG000049695.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049601.1 SMESG000049567.1 SMESG000040257.1 SMESG000022691.1 SMESG000022676.1 SMESG000049548.1 SMESG000049895.1 SMESG000049844.1 SMESG000049812.1 SMESG000022696.1 SMESG000022690.1 SMESG000022619.1 SMESG000072955.1 SMESG000049806.1 SMESG000049805.1 SMESG000032755.1 SMESG000032715.1 SMESG000022665.1 SMESG000000563.1 SMESG000000371.1 SMESG000049772.1 SMESG000049767.1 SMESG000049739.1 SMESG000049688.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000022612.1 SMESG000029699.1 SMESG000000559.1 SMESG000000302.1 SMESG000049565.1 SMESG000065603.1 SMESG000000411.1 SMESG000000372.1 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014044SMESG000079607.1 SMESG000049847.1 SMESG000049832.1 SMESG000049756.1 SMESG000040301.1 SMESG000022640.1 SMESG000072592.1 SMESG000049745.1 SMESG000049740.1 SMESG000049695.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049601.1 SMESG000049567.1 SMESG000040257.1 SMESG000022691.1 SMESG000022676.1 SMESG000049548.1 SMESG000049895.1 SMESG000049844.1 SMESG000049812.1 SMESG000022696.1 SMESG000022690.1 SMESG000022619.1 SMESG000072955.1 SMESG000049806.1 SMESG000049805.1 SMESG000032755.1 SMESG000032715.1 SMESG000022665.1 SMESG000000563.1 SMESG000000371.1 SMESG000049772.1 SMESG000049767.1 SMESG000049739.1 SMESG000049688.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000022612.1 SMESG000029699.1 SMESG000000559.1 SMESG000000302.1 SMESG000049565.1 SMESG000065603.1 SMESG000000411.1 SMESG000000372.1 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30014044SMESG000079607.1 SMESG000049847.1 SMESG000049832.1 SMESG000049756.1 SMESG000040301.1 SMESG000022640.1 SMESG000072592.1 SMESG000049745.1 SMESG000049740.1 SMESG000049695.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049601.1 SMESG000049567.1 SMESG000040257.1 SMESG000022691.1 SMESG000022676.1 SMESG000049548.1 SMESG000049895.1 SMESG000049844.1 SMESG000049812.1 SMESG000022696.1 SMESG000022690.1 SMESG000022619.1 SMESG000072955.1 SMESG000049806.1 SMESG000049805.1 SMESG000032755.1 SMESG000032715.1 SMESG000022665.1 SMESG000000563.1 SMESG000000371.1 SMESG000049772.1 SMESG000049767.1 SMESG000049739.1 SMESG000049688.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000022612.1 SMESG000029699.1 SMESG000000559.1 SMESG000000302.1 SMESG000049565.1 SMESG000065603.1 SMESG000000411.1 SMESG000000372.1 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30014044SMESG000079607.1 SMESG000049847.1 SMESG000049832.1 SMESG000049756.1 SMESG000040301.1 SMESG000022640.1 SMESG000072592.1 SMESG000049745.1 SMESG000049740.1 SMESG000049695.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049601.1 SMESG000049567.1 SMESG000040257.1 SMESG000022691.1 SMESG000022676.1 SMESG000049548.1 SMESG000049895.1 SMESG000049844.1 SMESG000049812.1 SMESG000022696.1 SMESG000022690.1 SMESG000022619.1 SMESG000072955.1 SMESG000049806.1 SMESG000049805.1 SMESG000032755.1 SMESG000032715.1 SMESG000022665.1 SMESG000000563.1 SMESG000000371.1 SMESG000049772.1 SMESG000049767.1 SMESG000049739.1 SMESG000049688.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000022612.1 SMESG000029699.1 SMESG000000559.1 SMESG000000302.1 SMESG000049565.1 SMESG000065603.1 SMESG000000411.1 SMESG000000372.1 dd_Smed_v6_490_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30014044SMESG000079607.1 SMESG000072955.1 SMESG000049847.1 SMESG000049844.1 SMESG000049832.1 SMESG000049812.1 SMESG000049806.1 SMESG000049805.1 SMESG000049772.1 SMESG000049767.1 SMESG000049756.1 SMESG000049745.1 SMESG000049740.1 SMESG000049739.1 SMESG000049695.1 SMESG000049688.1 SMESG000049683.1 SMESG000049676.1 SMESG000049665.1 SMESG000049663.1 SMESG000049647.1 SMESG000049621.1 SMESG000049614.1 SMESG000049601.1 SMESG000049567.1 SMESG000049548.1 SMESG000040301.1 SMESG000040257.1 SMESG000032755.1 SMESG000032715.1 SMESG000022696.1 SMESG000022691.1 SMESG000022676.1 SMESG000022665.1 SMESG000022640.1 SMESG000022619.1 SMESG000022612.1 dd_Smed_v4_490_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30014044 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000499:42308..49254 +18297
Homology
[Close]
BLAST of SMED30014044 vs. Planmine SMEST
Match: SMESG000004451.1 (SMESG000004451.1)

HSP 1 Score: 468.774 bits (1205), Expect = 9.476e-157
Identity = 233/236 (98.73%), Postives = 233/236 (98.73%), Query Frame = 1 
Query: 2914 NMFIIR-CFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQQLGNLNEDHYPIYSLPSFSKNLKHINKKIVLIIDFGNYEKFTGENEVIFYTGLKNLLDNINQNFICMMTPEMNFQKNSSWKSFISNQIANVNIIITVFSSAMTQKLCEWESMGHSFQDIPETENKQDPLNIVWNKIKFSRKPVIGVVFDNTLEESASSIVPVVFYLPRNLKLLFGYLLSRMKDF 3618
            N F IR CFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQQLGNLNEDHYPIYSLPSFSKNLKHINKKIVLIIDFGNYEKFTGENEVIFYTGLKNLLDNINQNFICMMTPEMNFQKNSSWKSFISNQIANVNIIITVFSSAMTQKLCEWESMGHSFQDIPETENKQDPLNIVWNKIKFSRKPVIGVVFDNTLEESASSIVPVVFYLPRNLKLLFGYLLSRMKDF
Sbjct:   12 NHFNIRKCFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQQLGNLNEDHYPIYSLPSFSKNLKHINKKIVLIIDFGNYEKFTGENEVIFYTGLKNLLDNINQNFICMMTPEMNFQKNSSWKSFISNQIANVNIIITVFSSAMTQKLCEWESMGHSFQDIPETENKQDPLNIVWNKIKFSRKPVIGVVFDNTLEESASSIVPVVFYLPRNLKLLFGYLLSRMKDF 247
[Close]
BLAST of SMED30014044 vs. Planmine SMEST
Match: SMESG000004451.1 (SMESG000004451.1)

HSP 1 Score: 149.828 bits (377), Expect = 6.573e-42
Identity = 76/81 (93.83%), Postives = 77/81 (95.06%), Query Frame = 1 
Query: 2914 NMFIIR-CFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQQLGNLNEDHYPIYSLPSFSKNLKHINKKI 3153
            N F IR CFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQQLGNLNEDHYPIYSLPSFSKNLKHINK +
Sbjct:   12 NHFNIRKCFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQQLGNLNEDHYPIYSLPSFSKNLKHINKSL 92
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BLAST of SMED30014044 vs. Planmine SMEST
Match: SMESG000050479.1 (SMESG000050479.1)

HSP 1 Score: 55.8398 bits (133), Expect = 4.716e-12
Identity = 31/63 (49.21%), Postives = 38/63 (60.32%), Query Frame = 2 
Query: 1328 SCNACNVTYIGETVRHLCTQVLKNCQLNRLNHTSDHFRK-----YTSKKKECLISR*NYWEGI 1501
            +CN CN TYIGET RHLCT+VL +CQL  L + S+H R        S  +  L S  NYWE +
Sbjct: 2851 TCNGCNATYIGETSRHLCTRVLDHCQLKGLTNISEHNRGCKSEIGMSDFRILLRSFNNYWERV 2913

HSP 2 Score: 35.8094 bits (81), Expect = 4.716e-12
Identity = 17/27 (62.96%), Postives = 22/27 (81.48%), Query Frame = 3 
Query: 1503 EALLIRTLIPLISVQNPQSTGILNVFK 1583
            EALLIR+L P I+VQ+  +T +LNVFK
Sbjct: 2916 EALLIRSLDPKINVQSAVTTNVLNVFK 2942
[Close]
BLAST of SMED30014044 vs. Planmine SMEST
Match: SMESG000050479.1 (SMESG000050479.1)

HSP 1 Score: 55.8398 bits (133), Expect = 5.211e-12
Identity = 31/63 (49.21%), Postives = 38/63 (60.32%), Query Frame = 2 
Query: 1328 SCNACNVTYIGETVRHLCTQVLKNCQLNRLNHTSDHFRK-----YTSKKKECLISR*NYWEGI 1501
            +CN CN TYIGET RHLCT+VL +CQL  L + S+H R        S  +  L S  NYWE +
Sbjct: 1894 TCNGCNATYIGETSRHLCTRVLDHCQLKGLTNISEHNRGCKSEIGMSDFRILLRSFNNYWERV 1956

HSP 2 Score: 35.4242 bits (80), Expect = 5.211e-12
Identity = 17/27 (62.96%), Postives = 22/27 (81.48%), Query Frame = 3 
Query: 1503 EALLIRTLIPLISVQNPQSTGILNVFK 1583
            EALLIR+L P I+VQ+  +T +LNVFK
Sbjct: 1959 EALLIRSLDPKINVQSAVTTNVLNVFK 1985
[Close]
BLAST of SMED30014044 vs. Planmine SMEST
Match: SMESG000050479.1 (SMESG000050479.1)

HSP 1 Score: 55.8398 bits (133), Expect = 5.835e-12
Identity = 31/63 (49.21%), Postives = 38/63 (60.32%), Query Frame = 2 
Query: 1328 SCNACNVTYIGETVRHLCTQVLKNCQLNRLNHTSDHFRK-----YTSKKKECLISR*NYWEGI 1501
            +CN CN TYIGET RHLCT+VL +CQL  L + S+H R        S  +  L S  NYWE +
Sbjct: 1960 TCNGCNATYIGETSRHLCTRVLDHCQLKGLTNISEHNRGCKSEIGMSDFRILLRSFNNYWERV 2022

HSP 2 Score: 35.4242 bits (80), Expect = 5.835e-12
Identity = 17/27 (62.96%), Postives = 22/27 (81.48%), Query Frame = 3 
Query: 1503 EALLIRTLIPLISVQNPQSTGILNVFK 1583
            EALLIR+L P I+VQ+  +T +LNVFK
Sbjct: 2025 EALLIRSLDPKINVQSAVTTNVLNVFK 2051
The following BLAST results are available for this feature:
BLAST of SMED30014044 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30014044 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004451.19.476e-15798.73SMESG000004451.1[more]
SMESG000004451.16.573e-4293.83SMESG000004451.1[more]
SMESG000050479.14.716e-1249.21SMESG000050479.1[more]
SMESG000050479.15.211e-1249.21SMESG000050479.1[more]
SMESG000050479.15.835e-1249.21SMESG000050479.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30014044 ID=SMED30014044|Name=SMED30014044|organism=Schmidtea mediterranea sexual|type=transcript|length=3689bp
TATAAATCTTATATGGCGAAATATGAAGATAATGTGGAGTCTTTAGAGTC
AGTATCTCTAATTGATAAATTTAATCGCGCTCAATGGCGCTTTTGTCCTC
CTGAAGTTGATATTGAAATTTCAAAAGATTTACATATGGGTTTTCAAAAA
AAAGTGTATAGTACAGTTTATGAATTTCTTAAATGGAATATTAGGGAAAT
TAATTATATAAAAGACGTACAATGCGAGGATGTTATTATTAGTATGTTTA
ATCATATAAGGAAAACAAATTTAAATACGTGTCAGATAGTGTGTCGTGAT
CCAGATAATCATTTTAATATTAGAAACATTATATTCTCACACAGAAATAT
ATTTTATTATCTTTATGTGATTTGTGTAAACTTGGTCATGTAAATATCCT
AGAGAAAGAAATCGGGTCTGTTGAGCATAGTGGCTATCCAATCACCTGTA
TAACTTTATAACTTTCTATTCAAAAGGGACTGAACATCCGTTTTAAATGA
TGGAATATCTGGCTCAAAAATAATTCCAAACTTATCCTTCAGAGGACAAA
CTGAGATATTAAACAGTGCCGCTCGATGTATCATAGGTCATATTTCAAAC
GAATTGGAAAGTGGGCCATCTCACCTTTCCATAATTGCAAATGGATTCGT
GATGTTAGCGAATATGAACAACTCAGTATTCGCATCAGATATGTTACTAA
TGGAGATGTTGTAGAATGGTATATTTTATTTGAATATCGTTGTCACCTGG
ATGGGATGTGTAGCTAGACCATTGCTTCAAAAACTAGAAACGCGTGCCCA
CATAGCTCAGTGCTATGATAAAGCAGTTGTGATGATTCAGTGAGCACATT
TTCTGCGGTATTGCAAAAAATTAGTAAGCATCAACATTGACTATATCAGA
TCTTCTGTGTTAAGAATGAAACTAATGAAAAATAGAGAAAAGTCTACAGG
TGATTAGACTCCCAAGTGTGCAACCACAAATGGGAGTATAATAATGATAT
AATTTCTGCTATATTTAATCGGTTTGATTACATACTTACTCTGTTGGATG
CTTTATTGCGACATCTACAAAGGGAGGATAATTGAGCCATAGGTGGTGAA
GTGTGAACCTAAATGCTTACGAAAGGTTATGTAACTCGTACTTCATTTTC
CCATAACCGAATCTTCATCAGAGCGTTTTTTTTCTGTACTTCGACATTTG
AAGACCTATATAAGATTAACAATGAGCCAGATGAGACTGAGCATTGCCTG
CCTTTTAGCGATTCAAAACCTTGACCTTGTAGCATATGAAGAAGTGTAAG
TATTACGATAACCCAGAGAGAATAAGGTCATGTAATGCGTGTAATGTCAC
ATACATTGGAGAGACTGTCCGTCATCTCTGCACTCAAGTTCTTAAGAATT
GTCAACTGAATAGATTAAATCACACTAGTGATCATTTTAGAAAATATACG
AGTAAAAAAAAAGAATGTCTGATATCAAGATAAAATTATTGGGAAGGAAT
TTGAGGCGTTGTTGATAAGAACATTAATTCCTTTAATTAGTGTTCAAAAT
CCTCAGTCAACGGGCATTTTGAATGTATTTAAATAATTGAATGTGAATTG
TGTATATTATGTATTTGATGTAATTTTTTGGGAGGTTAAAATGATTTTGT
TATCTAAAAAGAATTTTGAAATTTTATTGTTTTTGAAAGGAATGATAATT
TTTATGTTTTCTAAAATATAATTTACAAGTTTATATTGTTTGATTCGGTT
TTTTTTGAATTTGTAACTAAATATACTGAATAAAAAATGTTTATCAGTAT
TATACTTATTAAACACAAAACAGATAATTCGTAAATATAGAAATGGATGT
AAAAAATAATTTATATTGGCATAAGAAAAGTAAAGTTTATCTCGGGGTGC
GAATCTGAATTGATCAAAAGTAGTCTGCACTTTTTTCGAATTCATCATTT
AATTTTTCACTTCGTCTTTCAAATACATTATTGCATTCTTAATAATAGAG
AATTTTCATAAAATTTTTTGAATAAAGGAAGTCGTCCAAAATCTTCAAGG
CTTGGGATGTGCCATTGGCATTAATTTCTTCGAAATTTTCCTGATTCGAA
AATTCATTATCATCCTGTAGCATCATTTCATTCTCCAAATCTTCTTTTTT
CATCGTTGAAATATCCCGATCAATTTGAATAAATTCCTCGACATTAATTC
CATGGCCCTCTGCAAGTTTAATCAAATCTCCATTTACTGCCGGTTCTTCG
CTGGTTCCAGATACATTTTCATTGGCAATTGTAAGCTTAGCAGGATTGAA
ACAATTAATCATTGTTTTGGGTGTTATCTAATGCCACGCTGAATCAATAT
CTTTTAATGCGTCCCATAAAGTTATTTTATTTATGTTTACTTATTTTCAA
AAAGAAATATTTTTTACTCAAATAAATGTAAATTAAATTTTAAAATATTT
TCAAGCGGCGTCAAAACGCCGGGCTCAAACGTCACATGCAGGATATCTTT
GATATTGTCTTATAATTATTATAGAATTAAAAATAAGCTGAATTATTCTG
GCAAAAAAACATACGCTATAACATACGTTTTATCCAGTTTCACTTTATTT
GATTATCTATTCTTATTTAAATTTAAAAAATTACAACACCTTTATAAATA
AAATGGAAATTAAAACAAAAATTTAATTAGCCCATATTTGATCATCTTGA
CAACACTTTGTATGACTTATGTTGGTTTTGAAAAGTTATTGACGTCAAGG
TAAGTTGGAATCCTAACTAAGATATTTAAAAAATATTTGTCCAATTTGAC
ACTTTTGAAAATACCCTTTCATTCTAGTTTTTATTTATTATGTTATAATT
TTTATTAATAATTATTAGATATTACAATAATTAAATGATAATAAAAAAAA
TAAATTAGAATACAATATGTTTATTATCAGGTGCTTTCCTCTGTATAAAG
TATATCAAACTTTAAAAGCTATTGATTTGAATTCTTCAAACTATCTTTTA
ACAAGCGAACTTCGAAGAGAAGTTTTGAGAAGAATCAATAATGAAGAAAA
ATTTTTGGCGAGTCAACAACTTGGAAATCTCAATGAAGATCATTATCCAA
TATATTCTTTACCTTCATTTTCCAAAAATCTCAAACATATAAATAAAAAG
ATAGTATTAATAATTGATTTTGGCAATTACGAGAAGTTTACTGGAGAGAA
TGAAGTCATCTTCTATACTGGGTTAAAAAATTTGTTGGATAATATCAATC
AAAATTTTATATGTATGATGACACCCGAAATGAATTTTCAGAAAAATTCT
AGTTGGAAATCTTTTATTTCCAACCAAATAGCAAATGTCAACATAATAAT
AACTGTTTTTTCATCGGCGATGACTCAAAAATTATGCGAATGGGAATCAA
TGGGCCACTCATTTCAAGATATTCCAGAGACGGAAAATAAACAAGACCCA
TTAAATATTGTTTGGAATAAAATAAAATTTAGTAGGAAACCCGTAATTGG
TGTCGTTTTTGACAATACTCTGGAGGAATCTGCTTCTTCTATTGTACCTG
TTGTATTTTATTTGCCCAGGAATCTGAAATTACTTTTTGGTTATTTACTT
AGCCGTATGAAAGATTTTTAAATCTTTGTTTTTATATTAGTAAAAAATTT
TCTTTAGACTAATTTTCCTCATTCATTTTATACACACAC
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protein sequence of SMED30014044-orf-1

>SMED30014044-orf-1 ID=SMED30014044-orf-1|Name=SMED30014044-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=108bp
MINCFNPAKLTIANENVSGTSEEPAVNGDLIKLAEGHGINVEEFIQIDRD
ISTMKKEDLENEMMLQDDNEFSNQENFEEINANGTSQALKILDDFLYSKN
FMKILYY*
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protein sequence of SMED30014044-orf-2

>SMED30014044-orf-2 ID=SMED30014044-orf-2|Name=SMED30014044-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=235bp
MFIIRCFPLYKVYQTLKAIDLNSSNYLLTSELRREVLRRINNEEKFLASQ
QLGNLNEDHYPIYSLPSFSKNLKHINKKIVLIIDFGNYEKFTGENEVIFY
TGLKNLLDNINQNFICMMTPEMNFQKNSSWKSFISNQIANVNIIITVFSS
AMTQKLCEWESMGHSFQDIPETENKQDPLNIVWNKIKFSRKPVIGVVFDN
TLEESASSIVPVVFYLPRNLKLLFGYLLSRMKDF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR008906HATC_C_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity