mRNA-capping enzyme
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30014011 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of mRNA-capping enzyme vs. Ensembl Human
Match: RNGTT (RNA guanylyltransferase and 5'-phosphatase [Source:HGNC Symbol;Acc:HGNC:10073]) HSP 1 Score: 247.669 bits (631), Expect = 1.754e-72 Identity = 148/330 (44.85%), Postives = 197/330 (59.70%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V V VT ++ V+Q GFPG QPVSM +N+KLL K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ NS VG +F RL I+ EII R + K QE F + K F+DI T+ KL +G+F ++ HEMDGLIFQP K Y G+ LKWKPP NS+DF LK + +G P+ VG L+V G + PFA+I+ +++E ++ Y+NKI+EC + S W +R+RTDKS PN A + SI NPV
Sbjct: 232 FLEGVT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGK---YKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIK-VTKELKQ-YDNKIIECKFENNS--WVFMRQRTDKSFPNAYNTAMAVCNSISNPV 553 HSP 2 Score: 147.517 bits (371), Expect = 8.789e-37 Identity = 71/172 (41.28%), Postives = 112/172 (65.12%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY 566
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L + Y
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRY 177
BLAST of mRNA-capping enzyme vs. Ensembl Human
Match: RNGTT (RNA guanylyltransferase and 5'-phosphatase [Source:HGNC Symbol;Acc:HGNC:10073]) HSP 1 Score: 209.92 bits (533), Expect = 8.144e-59 Identity = 134/330 (40.61%), Postives = 182/330 (55.15%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V V VT ++ V+Q GFPG QPVSM +N+KLL K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ NS VG +F RL I+ EII R + K QE F + K F+DI T+ K ++P +D+ LKWKPP NS+DF LK + +G P+ VG L+V G + PFA+I+ +++E ++ Y+NKI+EC + S W +R+RTDKS PN A + SI NPV
Sbjct: 232 FLEGVT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKK-----------------YKPGRCDDI---------LKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIK-VTKELKQ-YDNKIIECKFENNS--WVFMRQRTDKSFPNAYNTAMAVCNSISNPV 530 HSP 2 Score: 147.517 bits (371), Expect = 7.612e-37 Identity = 71/172 (41.28%), Postives = 112/172 (65.12%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY 566
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L + Y
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRY 177
BLAST of mRNA-capping enzyme vs. Ensembl Human
Match: RNGTT (RNA guanylyltransferase and 5'-phosphatase [Source:HGNC Symbol;Acc:HGNC:10073]) HSP 1 Score: 156.762 bits (395), Expect = 9.810e-41 Identity = 101/253 (39.92%), Postives = 133/253 (52.57%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDG 1751
F+EGV+ V V VT ++ V+Q GFPG QPVSM +N+KLL K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ NS VG +F RL I+ EII R + K QE F + K F+DI T+ K ++P +D+ LKWKPP NS+DF LK + +G
Sbjct: 232 FLEGVT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKK-----------------YKPGRCDDI---------LKWKPPSLNSVDFRLKITRMGGEG 457 HSP 2 Score: 147.132 bits (370), Expect = 1.964e-37 Identity = 71/172 (41.28%), Postives = 112/172 (65.12%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY 566
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L + Y
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRY 177
BLAST of mRNA-capping enzyme vs. Ensembl Human
Match: DUSP11 (dual specificity phosphatase 11 [Source:HGNC Symbol;Acc:HGNC:3066]) HSP 1 Score: 104.76 bits (260), Expect = 1.647e-23 Identity = 75/258 (29.07%), Postives = 127/258 (49.22%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSFLAS--KKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYDFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQP---KETSRSPKS--HEIQDMEACEVERMETEVIDSA 788
+G P +W D +G + F+ FK PL + F+ ++ P FS +++E++G+I++LT T++YY +L + Y +IF GH+ PD + + KF + F ++ DK+IGVHCTHG NRTG+++ +L + + D A+ELF + R + RQ+Y++ L+ P R W S + + D ED+ L QP K + P+ H+IQ A +T+ + +
Sbjct: 73 KGGNHIPERWKDYLPVGQRMPGTRFIAFKVPLQKSFEKKLAPEECFSPLDLFNKIREQNEELGLIIDLTYTQRYYKPEDLPETVPYLKIFTVGHQV-PDDETIFKFKHAVNGFLKENKDNDKLIGVHCTHGLNRTGYLICRYLIDVEGVRPDDAIELFNRCRGHCLERQNYIEDLQN---------GPIRKNWNSSVPR----------SSDFEDSAHLMQPVHNKPVKQGPRYNLHQIQGHSAPRHFHTQTQSLQQS 310
BLAST of mRNA-capping enzyme vs. Ensembl Human
Match: DUSP11 (dual specificity phosphatase 11 [Source:HGNC Symbol;Acc:HGNC:3066]) HSP 1 Score: 82.8037 bits (203), Expect = 7.565e-17 Identity = 47/139 (33.81%), Postives = 79/139 (56.83%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSFLA--SKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMV 446
+G P +W D +G + F+ FK PL + F+ ++ P FS +++E++G+I++LT T++YY +L + Y +IF GH+ PD + + KF + F ++ DK+IGVHCTHG NRTG+++
Sbjct: 73 KGGNHIPERWKDYLPVGQRMPGTRFIAFKVPLQKSFEKKLAPEECFSPLDLFNKIREQNEELGLIIDLTYTQRYYKPEDLPETVPYLKIFTVGHQV-PDDETIFKFKHAVNGFLKENKDNDKLIGVHCTHGLNRTGYLI 210
BLAST of mRNA-capping enzyme vs. Ensembl Celegans
Match: cel-1 (mRNA-capping enzyme Polynucleotide 5'-triphosphatase mRNA guanylyltransferase [Source:UniProtKB/Swiss-Prot;Acc:Q17607]) HSP 1 Score: 225.328 bits (573), Expect = 7.911e-65 Identity = 135/334 (40.42%), Postives = 196/334 (58.68%), Query Frame = 3
Query: 1017 FMEG-VSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGF----PRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGIL---MKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIY----NKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
FM+G + V D + ++ ++NL K NK GFPG QPVS++ N+ LL ++ Y+VSWK DG+RY+I + D VY DRDN VF +E L F ++D E++ID V+ G PR LI+DI+ NSVNV F R +IK+EII+ R + + + I+ KDF+D++ T KL+ F + HE+DGLIFQP K+ Y +G+ LKWKPP +NS+DF LK E K+G P ++G LFV +PF ++ + +KY+NKI+EC K+W+ +RERTDKS PN + A N++E+++NPV
Sbjct: 238 FMDGLIRGVKVCEDEGKKSMLQAKIKNLCKYNKQGFPGLQPVSLSRGNINLLEQESYMVSWKADGMRYIIYINDGD-VYAFDRDNEVFEIENLDFVTKNGAPLMETLVDTEVIIDKVEINGAMCDQPRMLIYDIMRFNSVNVMKEPFYKRFEIIKTEIIDMRTAAFKTGRLKHENQIMSVRRKDFYDLEATAKLFGPKFVQHVGHEIDGLIFQP--KKTKYETGRCDKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGYLFVQNLSDPFGTMK--ATATLKKYHNKIIECTLLVDNQGRPKEWKFMRERTDKSLPNGLRTAENVVETMVNPV 566 HSP 2 Score: 147.132 bits (370), Expect = 3.428e-37 Identity = 76/194 (39.18%), Postives = 117/194 (60.31%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIP-------PANRFSLTSFLASKKSEKVGMIVNLTNTKKYY---NVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYD--FDGKLIAPKRPEW 608
P +WL CP GT+I+++F PFKTPL + +D +I PA FS +K+G+ ++LTNT +YY V E + I Y ++ + G P Q+ D FI + F ++ D+V+GVHCTHG+NRTGF++ ++L + + LD A+ FA+ R GI++QDY+D L YD D K++AP++P+W
Sbjct: 4 PDRWLHCPKTGTLINNLFFPFKTPLCKMYDNQIAERRYQFHPAEVFSHPHL----HGKKIGLWIDLTNTDRYYFREEVTEHECI--YHKMKMAGRGVSPTQEDTDNFIKLVQEFHKKYPDRVVGVHCTHGFNRTGFLIAAYLFQVEEYGLDAAIGEFAENRQKGIYKQDYIDDLFARYDPTEDDKILAPEKPDW 191
BLAST of mRNA-capping enzyme vs. Ensembl Celegans
Match: cel-1 (mRNA-capping enzyme Polynucleotide 5'-triphosphatase mRNA guanylyltransferase [Source:UniProtKB/Swiss-Prot;Acc:Q17607]) HSP 1 Score: 226.098 bits (575), Expect = 9.762e-65 Identity = 135/334 (40.42%), Postives = 196/334 (58.68%), Query Frame = 3
Query: 1017 FMEG-VSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGF----PRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGIL---MKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIY----NKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
FM+G + V D + ++ ++NL K NK GFPG QPVS++ N+ LL ++ Y+VSWK DG+RY+I + D VY DRDN VF +E L F ++D E++ID V+ G PR LI+DI+ NSVNV F R +IK+EII+ R + + + I+ KDF+D++ T KL+ F + HE+DGLIFQP K+ Y +G+ LKWKPP +NS+DF LK E K+G P ++G LFV +PF ++ + +KY+NKI+EC K+W+ +RERTDKS PN + A N++E+++NPV
Sbjct: 250 FMDGLIRGVKVCEDEGKKSMLQAKIKNLCKYNKQGFPGLQPVSLSRGNINLLEQESYMVSWKADGMRYIIYINDGD-VYAFDRDNEVFEIENLDFVTKNGAPLMETLVDTEVIIDKVEINGAMCDQPRMLIYDIMRFNSVNVMKEPFYKRFEIIKTEIIDMRTAAFKTGRLKHENQIMSVRRKDFYDLEATAKLFGPKFVQHVGHEIDGLIFQP--KKTKYETGRCDKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGYLFVQNLSDPFGTMKATA--TLKKYHNKIIECTLLVDNQGRPKEWKFMRERTDKSLPNGLRTAENVVETMVNPV 578 HSP 2 Score: 147.902 bits (372), Expect = 3.706e-37 Identity = 76/194 (39.18%), Postives = 117/194 (60.31%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIP-------PANRFSLTSFLASKKSEKVGMIVNLTNTKKYY---NVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYD--FDGKLIAPKRPEW 608
P +WL CP GT+I+++F PFKTPL + +D +I PA FS +K+G+ ++LTNT +YY V E + I Y ++ + G P Q+ D FI + F ++ D+V+GVHCTHG+NRTGF++ ++L + + LD A+ FA+ R GI++QDY+D L YD D K++AP++P+W
Sbjct: 16 PDRWLHCPKTGTLINNLFFPFKTPLCKMYDNQIAERRYQFHPAEVFSHPHL----HGKKIGLWIDLTNTDRYYFREEVTEHECI--YHKMKMAGRGVSPTQEDTDNFIKLVQEFHKKYPDRVVGVHCTHGFNRTGFLIAAYLFQVEEYGLDAAIGEFAENRQKGIYKQDYIDDLFARYDPTEDDKILAPEKPDW 203
BLAST of mRNA-capping enzyme vs. Ensembl Celegans
Match: cel-1 (mRNA-capping enzyme Polynucleotide 5'-triphosphatase mRNA guanylyltransferase [Source:UniProtKB/Swiss-Prot;Acc:Q17607]) HSP 1 Score: 226.098 bits (575), Expect = 9.762e-65 Identity = 135/334 (40.42%), Postives = 196/334 (58.68%), Query Frame = 3
Query: 1017 FMEG-VSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGF----PRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGIL---MKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIY----NKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
FM+G + V D + ++ ++NL K NK GFPG QPVS++ N+ LL ++ Y+VSWK DG+RY+I + D VY DRDN VF +E L F ++D E++ID V+ G PR LI+DI+ NSVNV F R +IK+EII+ R + + + I+ KDF+D++ T KL+ F + HE+DGLIFQP K+ Y +G+ LKWKPP +NS+DF LK E K+G P ++G LFV +PF ++ + +KY+NKI+EC K+W+ +RERTDKS PN + A N++E+++NPV
Sbjct: 250 FMDGLIRGVKVCEDEGKKSMLQAKIKNLCKYNKQGFPGLQPVSLSRGNINLLEQESYMVSWKADGMRYIIYINDGD-VYAFDRDNEVFEIENLDFVTKNGAPLMETLVDTEVIIDKVEINGAMCDQPRMLIYDIMRFNSVNVMKEPFYKRFEIIKTEIIDMRTAAFKTGRLKHENQIMSVRRKDFYDLEATAKLFGPKFVQHVGHEIDGLIFQP--KKTKYETGRCDKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGYLFVQNLSDPFGTMKATA--TLKKYHNKIIECTLLVDNQGRPKEWKFMRERTDKSLPNGLRTAENVVETMVNPV 578 HSP 2 Score: 147.902 bits (372), Expect = 3.706e-37 Identity = 76/194 (39.18%), Postives = 117/194 (60.31%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIP-------PANRFSLTSFLASKKSEKVGMIVNLTNTKKYY---NVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYD--FDGKLIAPKRPEW 608
P +WL CP GT+I+++F PFKTPL + +D +I PA FS +K+G+ ++LTNT +YY V E + I Y ++ + G P Q+ D FI + F ++ D+V+GVHCTHG+NRTGF++ ++L + + LD A+ FA+ R GI++QDY+D L YD D K++AP++P+W
Sbjct: 16 PDRWLHCPKTGTLINNLFFPFKTPLCKMYDNQIAERRYQFHPAEVFSHPHL----HGKKIGLWIDLTNTDRYYFREEVTEHECI--YHKMKMAGRGVSPTQEDTDNFIKLVQEFHKKYPDRVVGVHCTHGFNRTGFLIAAYLFQVEEYGLDAAIGEFAENRQKGIYKQDYIDDLFARYDPTEDDKILAPEKPDW 203
BLAST of mRNA-capping enzyme vs. Ensembl Celegans
Match: pir-1 (RNA/RNP complex-1-interacting phosphatase homolog [Source:UniProtKB/Swiss-Prot;Acc:Q22707]) HSP 1 Score: 90.1225 bits (222), Expect = 4.546e-20 Identity = 55/168 (32.74%), Postives = 91/168 (54.17%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDM-FVPFKTPLDQKF--DPEIPPANRFSLTSF--LASKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFI-VICSYFFDRFGD-KVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDY 542
P +W N+G I FVPFKTPLD F +P +F + + LA + ++++G++++LTNT +YY E D ++Y ++ GHE + V FI + + D+ D K+IGVHCTHG NRTG+++ ++ + + A+ +F R + R+ Y
Sbjct: 26 PDRWNIYDNVGRDIDGTRFVPFKTPLDSSFFDGKNMPVELQFGVKTLISLAQQANKQIGLVIDLTNTDRYYKKTEWADHGVKYLKLNCPGHEVNEREDLVQDFINAVKEFVNDKENDGKLIGVHCTHGLNRTGYLICRYMIDVDNYSASDAISMFEYYRGHPMEREHY 193
BLAST of mRNA-capping enzyme vs. Ensembl Celegans
Match: F54C8.4 (Probable tyrosine-protein phosphatase F54C8.4 [Source:UniProtKB/Swiss-Prot;Acc:P34442]) HSP 1 Score: 82.4185 bits (202), Expect = 1.311e-16 Identity = 46/172 (26.74%), Postives = 89/172 (51.74%), Query Frame = 3
Query: 63 PSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSFLA--SKKSEKVGMIVNLTNTKKYYNVNELDGI-IRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGD--KVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRK 560
P W +G +I F+ FKTP++ + +I RF+ S++ + +G++V+L++T +YY+ ++ G+ ++Y+++ G V+ F + + D+ D +IGVHCT+G NR G+++ FL ++ A++ F +AR I + Y+ L K
Sbjct: 9 PKDWSKFQPVGNVIPRTRFIVFKTPINSQLSTKIHKEQRFTTNDLFRQLSERGQYLGLVVDLSDTDRYYDKKDITGMCVQYEKVNCPGRGFIERDDCVESFHQVIQDYTDKCDDPDALIGVHCTNGINRCGYLICRFLIDRLGWSSHEAIDAFEQARGYSIEKGAYVMALHK 180
BLAST of mRNA-capping enzyme vs. Ensembl Fly
Match: mRNA-cap (gene:FBgn0030556 transcript:FBtr0073922) HSP 1 Score: 344.739 bits (883), Expect = 5.812e-109 Identity = 224/657 (34.09%), Postives = 339/657 (51.60%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIG-TIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKV--GMIVNLTNTKKYYNVNELDGI-IRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNF-PHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
RG+ P++WL CP TII + F+ FKTPL F ++P F K+ KV G+ V+LTNTK++Y+ + ++ + +Y ++ GH P +Q FI I F + VI VHCTHG+NRTGF++V +L E+ D + A+ +FA ARPPGI++QDY++ L K Y D + AP++P W L+ D + Q +S S ++ E QD +A EVE ED G + +S + + + I +ATF M GV V V D + ++++ V++ K GFPG QPVSM EN+K L++ Y VSWK DG RYM+++ G D VY DR++ F+VE + F S EH ++DGEMV+D + + PR+LI+DIV +++ +V F P+RL IK+E+I R + + ++F + KDFWDI + +L F L HE DGLIFQP+++ Y +G KWKP E NS+DF LK + +G + VG L+V G P+ +++ +++E RE +N+I+EC N+ W+ +RERTDK +PN A ++++SI +PV
Sbjct: 10 RGSGPLPNRWLYCPRKSDTIIAERFLAFKTPLSNNFHDKMPIECTFQPEMLFEYCKTLKVKLGLWVDLTNTKRFYDRSAVEELGAKYIKLQCRGHGETPSPEQTHSFIEIVDNFINERPFDVIAVHCTHGFNRTGFLIVCYLVERLDCSVSAALAIFASARPPGIYKQDYINELYKRYEDTNAAPAAPEQPNWC--------------LDYDDGNGDGFVQDNSSSTSQQAGE-QDDDAEEVEG-----------------------------------------------EDAGGDCDASTSDGQPRKKRRREMIIKNATF--------MAGVPGVRQVSDQPRLGDLQRKVQDWCDWKKNGFPGAQPVSMDRENIKRLSEIPYRVSWKADGTRYMMLIDGRDEVYFFDRNHSCFQVENVTFVESRNLNEHLDGTLVDGEMVLDKIGETVTPRYLIYDIVRLSNRDVRDEPFYPNRLDYIKTEVIGPRILGMKHGIINQRLQAFSVRGKDFWDIWMSARLLGEKFSRTLAHEPDGLIFQPSKQP--YTAGTCSDVFKWKPHELNSVDFRLKIITERGEGLLTKKVGFLYVGGHDAPYGRMQRLTKETRE-LDNRIVECTMNQFG-NWDFMRERTDKKNPNSYNTARSVVDSIKHPV 592
BLAST of mRNA-capping enzyme vs. Ensembl Fly
Match: CG13197 (gene:FBgn0062449 transcript:FBtr0088078) HSP 1 Score: 93.5893 bits (231), Expect = 2.733e-20 Identity = 54/173 (31.21%), Postives = 95/173 (54.91%), Query Frame = 3
Query: 57 DPPSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGD--KVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKI 563
D P +WL IG + F+ FK PL+Q + ++ R + S L + +G+I++LTNT +YY+ + + + + + ++ + G +T P K +F + F +R D K+IGVHCTHG NRTG+++ F+ ++ + A++ F+ AR I R +YL L+ +
Sbjct: 4 DIPDRWLKYKPIGDRVPGTRFIAFKVPLNQHVNAKVKENLRLAPESLL--QIVPDMGLIIDLTNTNRYYHPSAITNHDVLHQKLMIPGKQT-PSHKLAQRFCAFVTDFLERNADNDKLIGVHCTHGVNRTGYLICYFMISVMNMSPEEAIQTFSLARGHEIERDNYLSSLKTL 173
BLAST of mRNA-capping enzyme vs. Ensembl Fly
Match: CG13197 (gene:FBgn0062449 transcript:FBtr0345619) HSP 1 Score: 93.5893 bits (231), Expect = 2.733e-20 Identity = 54/173 (31.21%), Postives = 95/173 (54.91%), Query Frame = 3
Query: 57 DPPSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGD--KVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKI 563
D P +WL IG + F+ FK PL+Q + ++ R + S L + +G+I++LTNT +YY+ + + + + + ++ + G +T P K +F + F +R D K+IGVHCTHG NRTG+++ F+ ++ + A++ F+ AR I R +YL L+ +
Sbjct: 4 DIPDRWLKYKPIGDRVPGTRFIAFKVPLNQHVNAKVKENLRLAPESLL--QIVPDMGLIIDLTNTNRYYHPSAITNHDVLHQKLMIPGKQT-PSHKLAQRFCAFVTDFLERNADNDKLIGVHCTHGVNRTGYLICYFMISVMNMSPEEAIQTFSLARGHEIERDNYLSSLKTL 173
BLAST of mRNA-capping enzyme vs. Ensembl Zebrafish
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:405803]) HSP 1 Score: 241.121 bits (614), Expect = 3.271e-70 Identity = 138/329 (41.95%), Postives = 195/329 (59.27%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTE--HHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKR---KFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGVS VS V ++ +++ + + ++ GFPG QPVSM +N+++L + Y VSWK DG RYM+++ G + VYM+DRDN VF +E L FP H SN +LDGEM+ID V PR+LI+DI+ + VG +F RL I+ EII R L Q KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y G+ LKWKPP +NS+DF LK V +G P+ VG L+V PFA+++ +D ++Y+NKI+EC + ++ W +R+R DKS PN A + SI +PV
Sbjct: 236 FLEGVSVKGVSQVTTQPKLGEIQRKCQQFSEWDRSGFPGAQPVSMDRKNIRMLEQNGYKVSWKADGTRYMMLIDGRNEVYMIDRDNSVFHIENLEFPFRKDLRIHLSNTLLDGEMIIDKVNGQPVPRYLIYDIIKFSGQPVGQCDFNRRLLCIEKEIISPRFEKMKLGQIDKAKEPFSVRNKPFFDIHAARKLLEGSFTSQVSHEVDGLIFQPIGK---YKPGRCDDILKWKPPSHNSVDFRLKITKVGGEGLIPQTVGLLYVGNYDMPFAQMKIT--KDLKQYDNKIIECTF--VNNTWVFMRQRVDKSFPNAYDTAMAVCNSIQHPV 557 HSP 2 Score: 140.198 bits (352), Expect = 1.885e-34 Identity = 74/188 (39.36%), Postives = 119/188 (63.30%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
P +W +CP G + F+P KT L ++D ++P NRF L+++L S K K+G++V+LTNT ++Y+ +++ I+Y ++ +GH P + + FI +C +F ++ ++IGVHCTHG+NRTGF++ ++L EK D ++ AV FA+ARPPGI++ DYL L R+ D + AP PEW
Sbjct: 7 PPRWRNCPRRGQPVAGKFLPMKTMLGPRYDDKVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRADIEKEGIKYVKLSCKGHGECPTAETTEMFIRLCEHFIEKTPTELIGVHCTHGFNRTGFLICAYLVEKMDWSIEAAVAAFAQARPPGIYKGDYLKELFRRYGDVEDAPAAPPLPEWC 193
BLAST of mRNA-capping enzyme vs. Ensembl Zebrafish
Match: dusp11 (dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) [Source:ZFIN;Acc:ZDB-GENE-050417-226]) HSP 1 Score: 97.4413 bits (241), Expect = 1.512e-21 Identity = 57/169 (33.73%), Postives = 89/169 (52.66%), Query Frame = 3
Query: 63 PSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSF--LASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL 554
P +W D ++G I F+ FK PL Q F + + F L K+ +++G+I++LT T +YY +L + Y +IF GHE P+ + F +F + DK+IGVHCTHG NRTG+++ +L + + A+ LF ++R I RQ+YL L
Sbjct: 10 PDRWTDYTSLGKRIPGTRFIAFKVPLKQSFRRHLSESEVFGPFDLVRLLEKERQQLGLIIDLTFTTRYYRAEDLPDTLYYMKIFTAGHEV-PNDATILSFKKAVRHFLHDNASNDKLIGVHCTHGLNRTGYLICRYLIDVDGMMPQKAINLFNESRGHSIERQNYLQDL 177
BLAST of mRNA-capping enzyme vs. Ensembl Xenopus
Match: mgc147600 (RAS and EF-hand domain containing [Source:Xenbase;Acc:XB-GENE-1002559]) HSP 1 Score: 180.644 bits (457), Expect = 2.436e-48 Identity = 124/333 (37.24%), Postives = 175/333 (52.55%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHS--NLILDG----EMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V+ + ++ +++ + GFPG QPVSM N+K L +K Y VSWKP YM ++ +N+ E +FP + HHS N L+ EM+ID V PR+LI+DI+ N VG +F RL I+ EII R + KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y +G+ LKWKPP NS+DF LK V +G R VG L+V PF++I+ +D ++Y NKI+EC + + +W +R+R DKS PN A + SI NPV
Sbjct: 233 FLEGVTVKHVNQITTEPKLGEIQRKCQQFCSWRGSGFPGAQPVSMDRSNMKFLEQKAYKVSWKP----YMPLL----------SENYC--SENYHFPANWRLHHSRANTYLNKSHCKEMIIDKVNGQVVPRYLIYDIIKFNGQPVGDCDFNIRLSCIEKEIISPRHEKMKTGLIDKAKEPFSVRNKPFFDIHAARKLLEGSFAREVSHEVDGLIFQPIGK---YKAGRCDDILKWKPPNLNSVDFLLKITKVGGEGLLTRNVGLLYVGKYDCPFSEIK--VTKDLKQYENKIIECKFE--NNRWVFMRQRVDKSFPNSYDTALAVCNSIRNPV 542 HSP 2 Score: 161.77 bits (408), Expect = 1.214e-41 Identity = 81/192 (42.19%), Postives = 122/192 (63.54%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEE 623
P +WL+CP G + F+P KT L K+D ++P NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P Q+ D F+ +C F DR ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L Y D + P+ P+W EE
Sbjct: 7 PPRWLNCPRRGQPVAAKFLPLKTMLGPKYDDQVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRNDIEKEGIKYIKLQCKGHGECPSQENTDTFLRLCERFIDRNPTELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKADYLKELFHRYGDIEDAPKPPELPDWCFEEE 197
BLAST of mRNA-capping enzyme vs. Ensembl Xenopus
Match: mgc147600 (RAS and EF-hand domain containing [Source:Xenbase;Acc:XB-GENE-1002559]) HSP 1 Score: 175.637 bits (444), Expect = 1.417e-46 Identity = 121/327 (37.00%), Postives = 170/327 (51.99%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V+ + ++ +++ + GFPG QPVSM N+K L +K Y VSWK D + I S Y+ +R F V +P + GEM+ID V PR+LI+DI+ N VG +F RL I+ EII R + KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y +G+ LKWKPP NS+DF LK V +G R VG L+V PF++I+ +D ++Y NKI+EC + + +W +R+R DKS PN A + SI NPV
Sbjct: 233 FLEGVTVKHVNQITTEPKLGEIQRKCQQFCSWRGSGFPGAQPVSMDRSNMKFLEQKAYKVSWKADD--KALSWISS---YLYNRS---FSVSHSASTSTPQP-----LSVGEMIIDKVNGQVVPRYLIYDIIKFNGQPVGDCDFNIRLSCIEKEIISPRHEKMKTGLIDKAKEPFSVRNKPFFDIHAARKLLEGSFAREVSHEVDGLIFQPIGK---YKAGRCDDILKWKPPNLNSVDFLLKITKVGGEGLLTRNVGLLYVGKYDCPFSEIK--VTKDLKQYENKIIECKFE--NNRWVFMRQRVDKSFPNSYDTALAVCNSIRNPV 539 HSP 2 Score: 161.77 bits (408), Expect = 1.104e-41 Identity = 81/192 (42.19%), Postives = 122/192 (63.54%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEE 623
P +WL+CP G + F+P KT L K+D ++P NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P Q+ D F+ +C F DR ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L Y D + P+ P+W EE
Sbjct: 7 PPRWLNCPRRGQPVAAKFLPLKTMLGPKYDDQVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRNDIEKEGIKYIKLQCKGHGECPSQENTDTFLRLCERFIDRNPTELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKADYLKELFHRYGDIEDAPKPPELPDWCFEEE 197
BLAST of mRNA-capping enzyme vs. Ensembl Xenopus
Match: mgc147600 (RAS and EF-hand domain containing [Source:Xenbase;Acc:XB-GENE-1002559]) HSP 1 Score: 171.014 bits (432), Expect = 3.218e-45 Identity = 117/327 (35.78%), Postives = 161/327 (49.24%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V+ + ++ +++ + GFPG QPVSM N+K L +K Y VSWK DG R D N EM+ID V PR+LI+DI+ N VG +F RL I+ EII R + KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y +G+ LKWKPP NS+DF LK V +G R VG L+V PF++I+ +D ++Y NKI+EC + + +W +R+R DKS PN A + SI NPV
Sbjct: 213 FLEGVTVKHVNQITTEPKLGEIQRKCQQFCSWRGSGFPGAQPVSMDRSNMKFLEQKAYKVSWKADGTR--------------DGKNQSL----------------------EMIIDKVNGQVVPRYLIYDIIKFNGQPVGDCDFNIRLSCIEKEIISPRHEKMKTGLIDKAKEPFSVRNKPFFDIHAARKLLEGSFAREVSHEVDGLIFQPIGK---YKAGRCDDILKWKPPNLNSVDFLLKITKVGGEGLLTRNVGLLYVGKYDCPFSEIK--VTKDLKQYENKIIECKFE--NNRWVFMRQRVDKSFPNSYDTALAVCNSIRNPV 496 HSP 2 Score: 145.976 bits (367), Expect = 1.773e-36 Identity = 75/175 (42.86%), Postives = 113/175 (64.57%), Query Frame = 3
Query: 114 FVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEE 623
F+P KT L K+D ++P NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P Q+ D F+ +C F DR ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L Y D + P+ P+W EE
Sbjct: 4 FLPLKTMLGPKYDDQVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRNDIEKEGIKYIKLQCKGHGECPSQENTDTFLRLCERFIDRNPTELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKADYLKELFHRYGDIEDAPKPPELPDWCFEEE 177
BLAST of mRNA-capping enzyme vs. Ensembl Xenopus
Match: mgc147600 (RAS and EF-hand domain containing [Source:Xenbase;Acc:XB-GENE-1002559]) HSP 1 Score: 171.014 bits (432), Expect = 4.532e-45 Identity = 117/327 (35.78%), Postives = 161/327 (49.24%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V+ + ++ +++ + GFPG QPVSM N+K L +K Y VSWK DG R D N EM+ID V PR+LI+DI+ N VG +F RL I+ EII R + KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y +G+ LKWKPP NS+DF LK V +G R VG L+V PF++I+ +D ++Y NKI+EC + + +W +R+R DKS PN A + SI NPV
Sbjct: 233 FLEGVTVKHVNQITTEPKLGEIQRKCQQFCSWRGSGFPGAQPVSMDRSNMKFLEQKAYKVSWKADGTR--------------DGKNQSL----------------------EMIIDKVNGQVVPRYLIYDIIKFNGQPVGDCDFNIRLSCIEKEIISPRHEKMKTGLIDKAKEPFSVRNKPFFDIHAARKLLEGSFAREVSHEVDGLIFQPIGK---YKAGRCDDILKWKPPNLNSVDFLLKITKVGGEGLLTRNVGLLYVGKYDCPFSEIK--VTKDLKQYENKIIECKFE--NNRWVFMRQRVDKSFPNSYDTALAVCNSIRNPV 516 HSP 2 Score: 161.384 bits (407), Expect = 1.053e-41 Identity = 81/192 (42.19%), Postives = 122/192 (63.54%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEE 623
P +WL+CP G + F+P KT L K+D ++P NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P Q+ D F+ +C F DR ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L Y D + P+ P+W EE
Sbjct: 7 PPRWLNCPRRGQPVAAKFLPLKTMLGPKYDDQVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRNDIEKEGIKYIKLQCKGHGECPSQENTDTFLRLCERFIDRNPTELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKADYLKELFHRYGDIEDAPKPPELPDWCFEEE 197
BLAST of mRNA-capping enzyme vs. Ensembl Xenopus
Match: mgc147600 (RAS and EF-hand domain containing [Source:Xenbase;Acc:XB-GENE-1002559]) HSP 1 Score: 169.088 bits (427), Expect = 2.514e-44 Identity = 116/327 (35.47%), Postives = 167/327 (51.07%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V+ + ++ +++ + GFPG QPVSM N+K L +K Y VSWK D ++V+ V R ++++ EM+ID V PR+LI+DI+ N VG +F RL I+ EII R + KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y +G+ LKWKPP NS+DF LK V +G R VG L+V PF++I+ +D ++Y NKI+EC + + +W +R+R DKS PN A + SI NPV
Sbjct: 233 FLEGVTVKHVNQITTEPKLGEIQRKCQQFCSWRGSGFPGAQPVSMDRSNMKFLEQKAYKVSWKAD-----VLVVS------------VPRWDKVF----------------EMIIDKVNGQVVPRYLIYDIIKFNGQPVGDCDFNIRLSCIEKEIISPRHEKMKTGLIDKAKEPFSVRNKPFFDIHAARKLLEGSFAREVSHEVDGLIFQPIGK---YKAGRCDDILKWKPPNLNSVDFLLKITKVGGEGLLTRNVGLLYVGKYDCPFSEIK--VTKDLKQYENKIIECKFE--NNRWVFMRQRVDKSFPNSYDTALAVCNSIRNPV 519 HSP 2 Score: 160.999 bits (406), Expect = 1.463e-41 Identity = 81/192 (42.19%), Postives = 122/192 (63.54%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEE 623
P +WL+CP G + F+P KT L K+D ++P NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P Q+ D F+ +C F DR ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L Y D + P+ P+W EE
Sbjct: 7 PPRWLNCPRRGQPVAAKFLPLKTMLGPKYDDQVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRNDIEKEGIKYIKLQCKGHGECPSQENTDTFLRLCERFIDRNPTELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKADYLKELFHRYGDIEDAPKPPELPDWCFEEE 197
BLAST of mRNA-capping enzyme vs. Ensembl Mouse
Match: Rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:MGI Symbol;Acc:MGI:1329041]) HSP 1 Score: 242.662 bits (618), Expect = 8.368e-71 Identity = 143/330 (43.33%), Postives = 196/330 (59.39%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EG++ V V VT ++ V+Q GFPG QPVSM +N++LL +K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ N+ VG +F RL I+ EII R + K QE F + K F+DI + KL +G+F ++ HEMDGLIFQP K Y G+ LKWKPP NS+DF LK + +G P+ VG L+V G + PFA+I+ +++E ++ Y+NKI+EC + S W +R+R DKS PN A + SI NPV
Sbjct: 232 FLEGIT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIRLLEQKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRPKQFFDINISRKLLEGNFAKEVSHEMDGLIFQPIGK---YKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIK-VTKELKQ-YDNKIIECKFENNS--WVFMRQRIDKSFPNAYNTAMAVCNSISNPV 553 HSP 2 Score: 148.288 bits (373), Expect = 3.892e-37 Identity = 74/188 (39.36%), Postives = 117/188 (62.23%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+ ++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L R+ D + P P+W
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMSLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERSPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPVLPDWC 193
BLAST of mRNA-capping enzyme vs. Ensembl Mouse
Match: Rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:MGI Symbol;Acc:MGI:1329041]) HSP 1 Score: 235.728 bits (600), Expect = 2.284e-68 Identity = 138/324 (42.59%), Postives = 193/324 (59.57%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIME 1964
F+EG++ V V VT ++ V+Q GFPG QPVSM +N++LL +K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ N+ VG +F RL I+ EII R + K QE F + K F+DI + KL +G+F ++ HEMDGLIFQP K Y G+ LKWKPP NS+DF LK + +G P+ VG L+V G + PFA+I+ +++E ++ Y+NKI+EC + S W +R+R DKS PN A ++ +
Sbjct: 232 FLEGIT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIRLLEQKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRPKQFFDINISRKLLEGNFAKEVSHEMDGLIFQPIGK---YKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIK-VTKELKQ-YDNKIIECKFENNS--WVFMRQRIDKSFPNAYNTAMDVQQ 547 HSP 2 Score: 148.288 bits (373), Expect = 3.182e-37 Identity = 74/188 (39.36%), Postives = 117/188 (62.23%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+ ++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L R+ D + P P+W
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMSLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERSPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPVLPDWC 193
BLAST of mRNA-capping enzyme vs. Ensembl Mouse
Match: Dusp11 (dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) [Source:MGI Symbol;Acc:MGI:1919352]) HSP 1 Score: 107.842 bits (268), Expect = 4.132e-25 Identity = 61/171 (35.67%), Postives = 98/171 (57.31%), Query Frame = 3
Query: 63 PSKWLDCPNIGTII-HDMFVPFKTPLDQKFDPEIPPANRFSLTSFLA--SKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRK 560
P +W D +G + F+ FK PL +KF+ ++ P FS +++E++G+I++LT T++YY V +L I Y +IF GH+ PD + +F F ++ DK+IGVHCTHG NRTG+++ +L + + D A+ELF R I RQ+Y++ L+K
Sbjct: 31 PERWKDYLPVGQRMPGTRFIAFKVPLQKKFEAKLMPEECFSPLDLFNKIQEQNEELGLIIDLTYTQRYYKVEDLPETISYIKIFTVGHQI-PDNDTIFQFKCAVKEFLKKNKNNDKLIGVHCTHGLNRTGYLICRYLIDVEGMRPDDAIELFNSCRGHCIERQNYIENLQK 200
BLAST of mRNA-capping enzyme vs. Ensembl Mouse
Match: Dusp11 (dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) [Source:MGI Symbol;Acc:MGI:1919352]) HSP 1 Score: 85.5001 bits (210), Expect = 3.506e-19 Identity = 45/117 (38.46%), Postives = 71/117 (60.68%), Query Frame = 3
Query: 114 FVPFKTPLDQKFDPEIPPANRFSLTSFLA--SKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVS 452
F+ FK PL +KF+ ++ P FS +++E++G+I++LT T++YY V +L I Y +IF GH+ PD + +F F ++ DK+IGVHCTHG NRTG+++ S
Sbjct: 15 FIAFKVPLQKKFEAKLMPEECFSPLDLFNKIQEQNEELGLIIDLTYTQRYYKVEDLPETISYIKIFTVGHQI-PDNDTIFQFKCAVKEFLKKNKNNDKLIGVHCTHGLNRTGYLICS 130
BLAST of mRNA-capping enzyme vs. UniProt/SwissProt
Match: sp|O60942|MCE1_HUMAN (mRNA-capping enzyme OS=Homo sapiens OX=9606 GN=RNGTT PE=1 SV=1) HSP 1 Score: 247.669 bits (631), Expect = 8.423e-72 Identity = 148/330 (44.85%), Postives = 197/330 (59.70%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV+ V V VT ++ V+Q GFPG QPVSM +N+KLL K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ NS VG +F RL I+ EII R + K QE F + K F+DI T+ KL +G+F ++ HEMDGLIFQP K Y G+ LKWKPP NS+DF LK + +G P+ VG L+V G + PFA+I+ +++E ++ Y+NKI+EC + S W +R+RTDKS PN A + SI NPV
Sbjct: 232 FLEGVT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGK---YKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIK-VTKELKQ-YDNKIIECKFENNS--WVFMRQRTDKSFPNAYNTAMAVCNSISNPV 553 HSP 2 Score: 147.517 bits (371), Expect = 4.221e-36 Identity = 71/172 (41.28%), Postives = 112/172 (65.12%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY 566
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+G++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L + Y
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERNPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRY 177
BLAST of mRNA-capping enzyme vs. UniProt/SwissProt
Match: sp|O55236|MCE1_MOUSE (mRNA-capping enzyme OS=Mus musculus OX=10090 GN=Rngtt PE=1 SV=1) HSP 1 Score: 242.662 bits (618), Expect = 5.853e-70 Identity = 143/330 (43.33%), Postives = 196/330 (59.39%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EG++ V V VT ++ V+Q GFPG QPVSM +N++LL +K Y VSWK DG RYM+++ G++ V+M+DRDN VF V L FP H SN +LDGEM+ID V PR+LI+DI+ N+ VG +F RL I+ EII R + K QE F + K F+DI + KL +G+F ++ HEMDGLIFQP K Y G+ LKWKPP NS+DF LK + +G P+ VG L+V G + PFA+I+ +++E ++ Y+NKI+EC + S W +R+R DKS PN A + SI NPV
Sbjct: 232 FLEGIT-VKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIRLLEQKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRPKQFFDINISRKLLEGNFAKEVSHEMDGLIFQPIGK---YKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIK-VTKELKQ-YDNKIIECKFENNS--WVFMRQRIDKSFPNAYNTAMAVCNSISNPV 553 HSP 2 Score: 148.288 bits (373), Expect = 2.723e-36 Identity = 74/188 (39.36%), Postives = 117/188 (62.23%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
P +WL+CP G + F+P KT L ++D ++ NRF L+++L S K K+ ++V+LTNT ++Y+ N+++ I+Y ++ +GH P + + FI +C F +R ++IGVHCTHG+NRTGF++ +FL EK D ++ AV FA+ARPPGI++ DYL L R+ D + P P+W
Sbjct: 7 PPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKV-KMSLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERSPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPVLPDWC 193
BLAST of mRNA-capping enzyme vs. UniProt/SwissProt
Match: sp|Q6NY98|MCE1_DANRE (mRNA-capping enzyme OS=Danio rerio OX=7955 GN=rngtt PE=2 SV=1) HSP 1 Score: 241.121 bits (614), Expect = 2.469e-69 Identity = 138/329 (41.95%), Postives = 195/329 (59.27%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTE--HHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKR---KFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGVS VS V ++ +++ + + ++ GFPG QPVSM +N+++L + Y VSWK DG RYM+++ G + VYM+DRDN VF +E L FP H SN +LDGEM+ID V PR+LI+DI+ + VG +F RL I+ EII R L Q KA+E F + K F+DI KL +G F ++ HE+DGLIFQP K Y G+ LKWKPP +NS+DF LK V +G P+ VG L+V PFA+++ +D ++Y+NKI+EC + ++ W +R+R DKS PN A + SI +PV
Sbjct: 236 FLEGVSVKGVSQVTTQPKLGEIQRKCQQFSEWDRSGFPGAQPVSMDRKNIRMLEQNGYKVSWKADGTRYMMLIDGRNEVYMIDRDNSVFHIENLEFPFRKDLRIHLSNTLLDGEMIIDKVNGQPVPRYLIYDIIKFSGQPVGQCDFNRRLLCIEKEIISPRFEKMKLGQIDKAKEPFSVRNKPFFDIHAARKLLEGSFTSQVSHEVDGLIFQPIGK---YKPGRCDDILKWKPPSHNSVDFRLKITKVGGEGLIPQTVGLLYVGNYDMPFAQMKIT--KDLKQYDNKIIECTF--VNNTWVFMRQRVDKSFPNAYDTAMAVCNSIQHPV 557 HSP 2 Score: 140.198 bits (352), Expect = 1.423e-33 Identity = 74/188 (39.36%), Postives = 119/188 (63.30%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
P +W +CP G + F+P KT L ++D ++P NRF L+++L S K K+G++V+LTNT ++Y+ +++ I+Y ++ +GH P + + FI +C +F ++ ++IGVHCTHG+NRTGF++ ++L EK D ++ AV FA+ARPPGI++ DYL L R+ D + AP PEW
Sbjct: 7 PPRWRNCPRRGQPVAGKFLPMKTMLGPRYDDKVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRADIEKEGIKYVKLSCKGHGECPTAETTEMFIRLCEHFIEKTPTELIGVHCTHGFNRTGFLICAYLVEKMDWSIEAAVAAFAQARPPGIYKGDYLKELFRRYGDVEDAPAAPPLPEWC 193
BLAST of mRNA-capping enzyme vs. UniProt/SwissProt
Match: sp|Q17607|MCE1_CAEEL (mRNA-capping enzyme OS=Caenorhabditis elegans OX=6239 GN=cel-1 PE=1 SV=2) HSP 1 Score: 226.098 bits (575), Expect = 1.242e-63 Identity = 135/334 (40.42%), Postives = 196/334 (58.68%), Query Frame = 3
Query: 1017 FMEG-VSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGF----PRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGIL---MKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIY----NKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
FM+G + V D + ++ ++NL K NK GFPG QPVS++ N+ LL ++ Y+VSWK DG+RY+I + D VY DRDN VF +E L F ++D E++ID V+ G PR LI+DI+ NSVNV F R +IK+EII+ R + + + I+ KDF+D++ T KL+ F + HE+DGLIFQP K+ Y +G+ LKWKPP +NS+DF LK E K+G P ++G LFV +PF ++ + +KY+NKI+EC K+W+ +RERTDKS PN + A N++E+++NPV
Sbjct: 250 FMDGLIRGVKVCEDEGKKSMLQAKIKNLCKYNKQGFPGLQPVSLSRGNINLLEQESYMVSWKADGMRYIIYINDGD-VYAFDRDNEVFEIENLDFVTKNGAPLMETLVDTEVIIDKVEINGAMCDQPRMLIYDIMRFNSVNVMKEPFYKRFEIIKTEIIDMRTAAFKTGRLKHENQIMSVRRKDFYDLEATAKLFGPKFVQHVGHEIDGLIFQP--KKTKYETGRCDKVLKWKPPSHNSVDFLLKVEKKCKEGMLPEWIGYLFVQNLSDPFGTMKATA--TLKKYHNKIIECTLLVDNQGRPKEWKFMRERTDKSLPNGLRTAENVVETMVNPV 578 HSP 2 Score: 147.902 bits (372), Expect = 4.715e-36 Identity = 76/194 (39.18%), Postives = 117/194 (60.31%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIP-------PANRFSLTSFLASKKSEKVGMIVNLTNTKKYY---NVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYD--FDGKLIAPKRPEW 608
P +WL CP GT+I+++F PFKTPL + +D +I PA FS +K+G+ ++LTNT +YY V E + I Y ++ + G P Q+ D FI + F ++ D+V+GVHCTHG+NRTGF++ ++L + + LD A+ FA+ R GI++QDY+D L YD D K++AP++P+W
Sbjct: 16 PDRWLHCPKTGTLINNLFFPFKTPLCKMYDNQIAERRYQFHPAEVFSHPHL----HGKKIGLWIDLTNTDRYYFREEVTEHECI--YHKMKMAGRGVSPTQEDTDNFIKLVQEFHKKYPDRVVGVHCTHGFNRTGFLIAAYLFQVEEYGLDAAIGEFAENRQKGIYKQDYIDDLFARYDPTEDDKILAPEKPDW 203
BLAST of mRNA-capping enzyme vs. UniProt/SwissProt
Match: sp|Q6BT58|MCE1_DEBHA (mRNA-capping enzyme subunit alpha OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=CEG1 PE=3 SV=2) HSP 1 Score: 117.857 bits (294), Expect = 7.823e-27 Identity = 105/363 (28.93%), Postives = 172/363 (47.38%), Query Frame = 3
Query: 1041 SAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVK-LLTKKKYLVSWKPDGVRYMIVVIG----SDIVYMLDRDNFVFRVERLYFPMSPTE-----HHSNLILDGEMVIDIVKDIGFP--RFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQ--------DKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQ------------------KPRYVGNLFVNGQQ--EPFAKIEDISREDREKYNN-------KIMECIYNKISKK--WELIRERTDKSSPNYVTVANNIMESILNPV 1982
+LD E + +R V +L+ FPG QP+S ++ L K Y V K DG+R ++ +I + V+++ R+N + + ++FP++ E +H +LDGE+V++ K++ P RF IFD + IN ++ + P RL I ++++ P D +++ I+ + K + KL + D+L HE DGLIF AE Y+ G + LKWKP N++D+ K E + K Q KP + G E F K+ + ED EK N +I+EC K+S WE++R R DKS+ N+++V + I+ SI + V
Sbjct: 14 GTILDRNETQELRLMVADLLGRRNPSFPGAQPISFERYHLNDTLMNKDYYVCEKSDGLRCLLFIINHPERGEGVFLITRENDYYYIPNIHFPLTNNEEKGKTYHHGTLLDGELVLE-TKNVPEPVLRFCIFDALAINGKDITKRHLPKRLGYITEQVMK-----PFDNFKRKNPEIVNAPDFPFKVSFKLMTSSYHADDVLSKMDQLFHESDGLIFTCAETP--YVFGTDSTLLKWKPAHENTVDY--KMEMIFKKFQDPDLDPRDPDSTYTDYDSKPELIKLRVWKGGADYEDFTKLS-LENEDWEKLKNLRQPLQGRIVEC-RKKLSDPGFWEMLRFRNDKSNGNHISVVDKILHSIQDGV 364
BLAST of mRNA-capping enzyme vs. TrEMBL
Match: A0A084W2G9 (mRNA-capping enzyme OS=Anopheles sinensis OX=74873 GN=ZHAS_00012195 PE=3 SV=1) HSP 1 Score: 343.584 bits (880), Expect = 1.692e-105 Identity = 231/657 (35.16%), Postives = 332/657 (50.53%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIG-TIIHDMFVPFKTPLDQKFDPEIPPANRF--SLTSFLASKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPM--SPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNF-PHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
RG P +WL CP T+I F+ FKTPL Q+F+ +IP +F S+ L + ++G+ ++LTNT ++Y+ +E+ D +Y ++ GH P ++Q FI I F + + +GVHCTHG+NRTGF++VS++ EK D +D A+ FA+ARPPGI++ DY+ L R+ D + + P+ P W L D D +Q Q+ +A E E DSA K E+ E+ G +S+ + S+ S P+A F MEGV V+ + D + ++ VR + GF G QPVSM N++ LT+ Y VSWK DG RYM+++ +Y DRDN VF VE + FP P H ++ ++DGEMVID D PR+L++D++ + V F P RL LI E+I R Q ++ E FG+ +K FWDIK +E L F L HE DGLI+QP+ D Y G LKWKP NSIDF L + +K G PR +G L+V G ++ + +I+ +++E R K NNKI+EC Y + W L+RERTDKS PN A ++ ESI PV
Sbjct: 3 RGPGPIPHRWLHCPRKSDTLIAGRFLAFKTPLKQEFESQIPIECKFTPSMLVDLMRRHKVRIGLWIDLTNTNRFYDKHEIEDKGSQYIKLQCRGHGETPSREQAKAFIEIVEEFIENHPVEAVGVHCTHGFNRTGFLIVSYMVEKMDCAVDAALMAFAQARPPGIYKADYIRELFRRYGDEEDAPMPPELPAWC--------------LEYDDGDGQQQ---------------QEDDAVE------EQNDSARR---------------------------GKKRTREEGENSGNGGEASLPTKRSKGG---PSYNPNAVF--------MEGVPNVTLLQDKALIAKLQDRVRMMCGSKMQGFSGAQPVSMDMGNLRFLTQMPYRVSWKADGTRYMMLIHREKEIYFFDRDNSVFAVEGIRFPSLEDPNRHLADTLVDGEMVIDKYGDQLTPRYLVYDVIYFQNREVRTLPFHPDRLTLIDRELIASRTRAIQKGLIDRSIEPFGVRLKQFWDIKQSEALLGPKFTKTLGHEPDGLIYQPS--IDPYTPGVCKRVLKWKPHHMNSIDFRLVIQEERKLGMVPRKIGLLYVGGMEQSYGEIK-LTKELR-KLNNKIIECKYQE-GLGWVLMRERTDKSFPNSYETARSVWESIRRPV 581
BLAST of mRNA-capping enzyme vs. TrEMBL
Match: Q9VY44 (mRNA-capping enzyme OS=Drosophila melanogaster OX=7227 GN=mRNA-cap PE=2 SV=1) HSP 1 Score: 344.739 bits (883), Expect = 1.727e-105 Identity = 224/657 (34.09%), Postives = 339/657 (51.60%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIG-TIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKV--GMIVNLTNTKKYYNVNELDGI-IRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNF-PHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
RG+ P++WL CP TII + F+ FKTPL F ++P F K+ KV G+ V+LTNTK++Y+ + ++ + +Y ++ GH P +Q FI I F + VI VHCTHG+NRTGF++V +L E+ D + A+ +FA ARPPGI++QDY++ L K Y D + AP++P W L+ D + Q +S S ++ E QD +A EVE ED G + +S + + + I +ATF M GV V V D + ++++ V++ K GFPG QPVSM EN+K L++ Y VSWK DG RYM+++ G D VY DR++ F+VE + F S EH ++DGEMV+D + + PR+LI+DIV +++ +V F P+RL IK+E+I R + + ++F + KDFWDI + +L F L HE DGLIFQP+++ Y +G KWKP E NS+DF LK + +G + VG L+V G P+ +++ +++E RE +N+I+EC N+ W+ +RERTDK +PN A ++++SI +PV
Sbjct: 10 RGSGPLPNRWLYCPRKSDTIIAERFLAFKTPLSNNFHDKMPIECTFQPEMLFEYCKTLKVKLGLWVDLTNTKRFYDRSAVEELGAKYIKLQCRGHGETPSPEQTHSFIEIVDNFINERPFDVIAVHCTHGFNRTGFLIVCYLVERLDCSVSAALAIFASARPPGIYKQDYINELYKRYEDTNAAPAAPEQPNWC--------------LDYDDGNGDGFVQDNSSSTSQQAGE-QDDDAEEVEG-----------------------------------------------EDAGGDCDASTSDGQPRKKRRREMIIKNATF--------MAGVPGVRQVSDQPRLGDLQRKVQDWCDWKKNGFPGAQPVSMDRENIKRLSEIPYRVSWKADGTRYMMLIDGRDEVYFFDRNHSCFQVENVTFVESRNLNEHLDGTLVDGEMVLDKIGETVTPRYLIYDIVRLSNRDVRDEPFYPNRLDYIKTEVIGPRILGMKHGIINQRLQAFSVRGKDFWDIWMSARLLGEKFSRTLAHEPDGLIFQPSKQP--YTAGTCSDVFKWKPHELNSVDFRLKIITERGEGLLTKKVGFLYVGGHDAPYGRMQRLTKETRE-LDNRIVECTMNQFG-NWDFMRERTDKKNPNSYNTARSVVDSIKHPV 592
BLAST of mRNA-capping enzyme vs. TrEMBL
Match: A0A1I8NM93 (mRNA-capping enzyme OS=Stomoxys calcitrans OX=35570 GN=106087451 PE=3 SV=1) HSP 1 Score: 345.51 bits (885), Expect = 1.942e-105 Identity = 233/664 (35.09%), Postives = 334/664 (50.30%), Query Frame = 3
Query: 30 MARGYRGNFDPPSKWLDCPNIG-TIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKS--EKVGMIVNLTNTKKYYN---VNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPM--SPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNF-PHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
M+ G RG P++WL CP II F+ FKTPL FD ++P F KS K+G+ ++LTNTK++Y+ V D +Y ++ GH P +Q FI I F + +IGVHCTHG+NRTGF++ S+L E+ D ++ A+ FAK RPPGI++ DY+ L R+ D D AP+ P W ++ +D+ + Q ++ S S Q A R +T S +L + PE + + + E A ED G E +K KFMEGV V+ V D + +++Q V+++ NK GFPG QPVSM EN+K L + Y VSWK DG RYM+++ D +Y +DR+N F+VE + F + EH + +LDGEMV+D + PR+LI+DI+ I S +G +F P+RL I E+I R + + E F + KDFWDI+ + L F LLHE DGLIFQP+E+ Y +G KWKP NS+DF LK G PR + L+V G + PFA+++ + +NKI+EC N +WEL+RERTDK+ PN A ++++SI PV
Sbjct: 1 MSNGNRGPGPIPNRWLHCPRKSEGIIVGKFIAFKTPLSAAFDDQMPDDCLFYPEMIFGYCKSIKLKLGLWIDLTNTKRFYDSAVVKSKD--TQYVKLQCRGHGETPSPEQTQSFIEIVDNFINERPSDLIGVHCTHGFNRTGFLIASYLVERFDFSVEAALATFAKFRPPGIYKADYIRELYRRFDDEDDAPPAPELPGWC----------------LEFDDSDRGPQKRKYEESAHSSNSQQAMAA---RSQTH---SNGSLGLIETLDDPEDEAQEDEGEANEGNEA---------EDGGAPNGKPRKKRRREMIIKNA--------------KFMEGVPGVTLVTDQPRLGDIQQKVQDMCGWNKSGFPGCQPVSMDCENMKRLHEIPYRVSWKADGTRYMMLINKRDEIYFIDRNNSCFQVENITFVKYNNLHEHLEDTLLDGEMVLDKYQGQHIPRYLIYDIIKIGSREIGKEHFYPNRLQCIDMEVISPRHKAIEKGIIDRPSEPFSLRKKDFWDIRQSASLLGEKFAKSLLHEPDGLIFQPSEQ--AYTAGVCKDVFKWKPLNMNSVDFRLKVGEESGQGILPRKICLLYVGGHEPPFAQMKYTKAIKAQNLDNKIVECTVN-AQGQWELMRERTDKTLPNSFNTAKSVIDSIRTPV 614
BLAST of mRNA-capping enzyme vs. TrEMBL
Match: B4R4N6 (mRNA-capping enzyme OS=Drosophila simulans OX=7240 GN=Dsim\GD15855 PE=3 SV=1) HSP 1 Score: 343.199 bits (879), Expect = 7.707e-105 Identity = 223/657 (33.94%), Postives = 341/657 (51.90%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIG-TIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKV--GMIVNLTNTKKYYNVNELDGI-IRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNF-PHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
RG+ P++WL CP TII + F+ FKTPL KF ++P F K+ KV G+ V+LTNTK++Y+ + ++ + +Y ++ GH P +Q FI I F + VI VHCTHG+NRTGF++V ++ E+ D + A+ +FA ARPPGI++QDY++ L K Y D + AP++P W ++ D + Q +S S ++ E QD +A EV+ ED G + +S + + + + +ATF M GV V V D + ++++ V++ K GFPG QPVSM EN+K L++ Y VSWK DG RYM+++ G D VY DR++ F+VE + F S EH ++DGEMV+D + + PR+LI+DIV +++ +V F P+RL IK+E+I R + + ++F + KDFWDI + +L F L HE DGLIFQP+++ Y +G KWKP E NS+DF LK + +G + VG L+V G+ P+ +++ +++E RE +N+I+EC N+ WE +RERTDK +PN T A ++ +SI +PV
Sbjct: 10 RGSGPLPNRWLYCPRKSDTIIAERFLAFKTPLSSKFHDKMPIECTFQPEMLFEYCKTLKVKLGLWVDLTNTKRFYDRSAVEELGAKYIKLQCRGHGDAPSLEQTHSFIEIVDNFINERPFDVIAVHCTHGFNRTGFLIVCYMVERLDCSVSAALAIFAGARPPGIYKQDYVNELYKRYEDTNVAPAAPEQPNWC--------------MDYDDGNGDGYVQDNSSSTSQQAGE-QDDDAEEVDG-----------------------------------------------EDAGGDCDASTSDGQPRKKRRREMVVKNATF--------MAGVPGVRQVSDQPRLGDLQRKVQDWCDWKKNGFPGAQPVSMDRENIKRLSEIPYRVSWKADGTRYMMLIDGKDEVYFFDRNHSCFQVENVTFVESRNLNEHLDGTLVDGEMVLDKIGETVTPRYLIYDIVRLSNRDVREEPFYPNRLDYIKTEVIGPRILGMKHGIINQRLQAFSVRGKDFWDIWMSARLLGEKFSRTLAHEPDGLIFQPSKQP--YTAGTCFDVFKWKPHELNSVDFRLKIITERGEGLLTKKVGFLYVGGRDAPYGRMQKLTKETRE-LDNRIVECTMNQFG-NWEFMRERTDKKNPNSYTTARSVEDSIRHPV 592
BLAST of mRNA-capping enzyme vs. TrEMBL
Match: A0A4E0RTK9 (mRNA-capping enzyme OS=Fasciola hepatica OX=6192 GN=D915_005034 PE=3 SV=1) HSP 1 Score: 343.199 bits (879), Expect = 2.015e-104 Identity = 225/685 (32.85%), Postives = 352/685 (51.39%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNE-LDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRF-GDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYDFDGKLI-APKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFC-----PPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFP----------------MSPTE-------HHSNLILDGEMVI---------DIVKDI--GFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQ---ESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
P +WL+CP +G++I D+F+PFKTPLD KFD + P + F + + + K+G +++LT +K++YN E LD ++ +I +G+E P Q+QVD FI + + F D GD+ +GVHCTHG+NRTGFM++++L E+ + +D+AV++FA ARPPGI++ DYL+ L Y + AP P W + + + + +D + S + +S S M+ ++ R + + ++ S I SLP ++ P P +++ + + I S + ++ ++ C P P + + T EN ++ L F G+QPVS+T N++ L Y VS+K DG RY+++V G + VYM+DR NFV+RVE L+FP SP+E H N +LDGEMV+ I +++ G PRFLI+DIV +N VG F R +I ++I R +SF + K F +K TE+L +F L H DGLIFQP ++ Y+ G + LKWKPP N++DF K Y K G+ P YVG+L++ G P AK+ + +D+ + KI+EC + W+++R RTDK+ PNY ++ESI+ PV
Sbjct: 10 PPRWLNCPRMGSMIVDIFIPFKTPLDSKFDNFMEPEHIFHVDDVFQACEPYKLGFVLDLTKSKRFYNRREVLDMNCKHLKIECKGNEETPTQEQVDLFIRVVNQFLDSSSGDEKVGVHCTHGFNRTGFMIIAYLVEELNYGVDIAVKIFADARPPGIYKADYLEDLFTRYGNKEDCVPAPVLPAWCIEDGDGDRLHGSKRPHAPDDDFDRGSLKQTSSGS--------MDKTKLSRYDNSMAEAVS------IGSLPSQRYPTPPK--------GTPKLMDGVSHV---ITLDQDSPEAHEARELADRLFRMGGCMYENGQPVFPNAQSSEGEDDSSEPATSNENTKRFKHPLR------FRGSQPVSITQRNIEALISFDYCVSYKADGCRYLLLVSGPNQVYMIDRANFVYRVEVLHFPSVAWVKCVQQSGNIQSHSPSEFLSVADGHLRNTLLDGEMVLCHDPSKPPPSIHEEVTSGIPRFLIYDIVTLNGKPVGRSPFFERYSIIDKQVIWPRNTAGHLGLVNFGIQSFSVRRKQFRPLKETEELLKQEFIQSLDHVTDGLIFQPCGPDEFYVLGTCYQQLKWKPPHLNTVDFRCKIVYEAKVGEIPGYVGHLYLGGLNTPAAKLAHVGPKDK-VLDGKIVECSLIP-NLGWKVLRIRTDKTEPNYHKSGKAVIESIMFPV 661
BLAST of mRNA-capping enzyme vs. Ensembl Cavefish
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:405803]) HSP 1 Score: 188.734 bits (478), Expect = 1.504e-53 Identity = 110/263 (41.83%), Postives = 152/263 (57.79%), Query Frame = 3
Query: 1209 VRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSP--TEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRK---FLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
RYM+++ G D VYM+DR+N +F + L FP H +N +LDGEM+ID V PR+LI+DI+ IN VG +F RL I+ EII R + Q K +E F + K F+DI KL +G F ++ HE+DGLIFQP K Y G+ LKWKPP NS+DF LK V +G P+ VG L+V PFA+++ +S+E ++ Y+NKI+EC + + W +R+R DKS PN A + SI PV
Sbjct: 52 CRYMMLIDGKDEVYMIDRENSIFHISNLEFPFRKDMNAHLTNTLLDGEMIIDKVNGQPIPRYLIYDIIKINGQPVGQCDFSRRLLCIEKEIIFPRSEKIKMGQIDKTKEPFSVRNKPFFDINAARKLLEGSFTSQVSHEVDGLIFQPIGK---YRPGRCDDILKWKPPSLNSVDFRLKISKVGGEGLIPQTVGLLYVGNYDVPFAQMK-VSKELKQ-YDNKIIECTFK--NNTWVFMRQRVDKSFPNSYDTAMAVCNSIQYPV 307
BLAST of mRNA-capping enzyme vs. Ensembl Cavefish
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:405803]) HSP 1 Score: 134.035 bits (336), Expect = 3.897e-35 Identity = 73/189 (38.62%), Postives = 116/189 (61.38%), Query Frame = 3
Query: 60 PPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
PP +W +CP G + F+P KT L ++D +P NRF L+++L S K K+G++V+LTNT ++Y+ E++ ++Y ++ +GH P + FI +C +F + ++IGVHCTHG+NRTGF++ ++L EK D ++ AV FA+AR PGI++ DYL L R+ D D P+ P+W
Sbjct: 6 PPPRWRNCPRRGQPVAGKFLPMKTMLGPRYDDSVPEENRFHPSMLSNYLKSLKV-KMGLLVDLTNTTRFYDRAEIEKEGVKYVKLQCKGHGECPTAETTGMFIRLCEHFMQKNPTELIGVHCTHGFNRTGFLICAYLVEKMDWSIEAAVAAFAQARSPGIYKGDYLKELFRRYGDVDDTPAPPQLPDWC 193
BLAST of mRNA-capping enzyme vs. Ensembl Cavefish
Match: dusp11 (dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) [Source:ZFIN;Acc:ZDB-GENE-050417-226]) HSP 1 Score: 70.8626 bits (172), Expect = 5.897e-14 Identity = 43/145 (29.66%), Postives = 77/145 (53.10%), Query Frame = 3
Query: 45 RGNFDPPSKWLDCPNIGTIIHDM-FVPFKTPLDQKFDPEIPPANRFSLTSF--LASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCE 464
RG P++ + +G I+ F+ FK PL ++ D + RF + ++ +++G++++LT T +YY +L +Y +++ GH+ P K + F F ++ DK+IGVHCTHG NRTG++V +L +
Sbjct: 4 RGKNSTPTRSVLYTAVGKRIYGTRFIAFKVPLKRQLDQ----SERFGPADLKEIMEQEGQELGLVIDLTFTDRYYKPQDLPESCQYLKLYTVGHDV-PTDKIILSFKRAVHRFLQENQDNDKLIGVHCTHGLNRTGYLVCRYLID 143
BLAST of mRNA-capping enzyme vs. Ensembl Sea Lamprey
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:405803]) HSP 1 Score: 196.438 bits (498), Expect = 2.639e-55 Identity = 119/330 (36.06%), Postives = 178/330 (53.94%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFP--MSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EGV +V V +T ++ V++ + +G P QPVSM +N+ L+ K Y+VSWK DG RYM+++ G + V+M++RDN +F V L FP +P H +N +LDGEM++D V PR+LI+DIV N VG +F R I+ EIIE R + KA E F + K FWD+ + VY+ G+ LKWKPP NS+DF LK + +G + +G L+V + PFA+++ + ++ +Y+NKI+EC ++ + +W +R+RTDKS PN T A + ESI PV
Sbjct: 178 FLEGV-QVPGVTQMTTQHKISMVQRKGQEFCMWKGYGVPQAQPVSMDRKNLVFLSNKHYMVSWKADGTRYMMLIDGENGVFMINRDNIIFNVASLEFPNRKNPDSHINNTLLDGEMIVDKVNGQDVPRYLIYDIVKFNGQPVGQCDFRTRQQCIQREIIEPRHEKMKAGHINKAMEPFSVRAKPFWDV--------------------------TQSRSVYVPGRCTDMLKWKPPSQNSVDFRLKISKIGGEGLLTKTMGLLYVGQKDTPFAQMK--ATKELRQYDNKIIECKFH--NNEWLFMRQRTDKSFPNSFTTAMGVCESIQFPV 476 HSP 2 Score: 112.079 bits (279), Expect = 3.035e-26 Identity = 52/133 (39.10%), Postives = 82/133 (61.65%), Query Frame = 3
Query: 219 VGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWA 611
+G++++LTNT ++Y+ E+ D I Y +++ GH P + F+ C + + K+IGVHCTHG+NRTGF++ ++L EK D ++ AV F++ARPPGI++ DYLD L Y D + P P+W
Sbjct: 1 MGVLIDLTNTNRFYDKKEIEDAGITYFKLYCRGHGECPLPEHTQVFVKFCDNYIKKNPQKIIGVHCTHGFNRTGFLICAYLVEKLDWSVEAAVAEFSRARPPGIYKGDYLDDLFGRYGDSEDTPAPPPLPDWC 133
BLAST of mRNA-capping enzyme vs. Ensembl Sea Lamprey
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:405803]) HSP 1 Score: 157.532 bits (397), Expect = 1.810e-41 Identity = 88/215 (40.93%), Postives = 126/215 (58.60%), Query Frame = 3
Query: 1017 FMEGVSKVSAVLDVT---EMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFP--MSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPA 1637
F+EGV +V V +T ++ V++ + +G P QPVSM +N+ L+ K Y+VSWK DG RYM+++ G + V+M++RDN +F V L FP +P H +N +LDGEM++D V PR+LI+DIV N VG +F R I+ EIIE R + KA E F + K FWD+ + L +G F ++ HE+DGLIFQPA
Sbjct: 209 FLEGV-QVPGVTQMTTQHKISMVQRKGQEFCMWKGYGVPQAQPVSMDRKNLVFLSNKHYMVSWKADGTRYMMLIDGENGVFMINRDNIIFNVASLEFPNRKNPDSHINNTLLDGEMIVDKVNGQDVPRYLIYDIVKFNGQPVGQCDFRTRQQCIQREIIEPRHEKMKAGHINKAMEPFSVRAKPFWDVTQSRSLLEGKFVQQVSHEVDGLIFQPA 422 HSP 2 Score: 124.02 bits (310), Expect = 3.268e-30 Identity = 62/164 (37.80%), Postives = 100/164 (60.98%), Query Frame = 3
Query: 135 LDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWA 611
L ++D ++P RF+ L+ +L S+K E +G++++LTNT ++Y+ E+ D I Y +++ GH P + F+ C + + K+IGVHCTHG+NRTGF++ ++L EK D ++ AV F++ARPPGI++ DYLD L Y D + P P+W
Sbjct: 2 LSTRYDDQVPEECRFNPSMLSVYLKSRKVE-MGVLIDLTNTNRFYDKKEIEDAGITYFKLYCRGHGECPLPEHTQVFVKFCDNYIKKNPQKIIGVHCTHGFNRTGFLICAYLVEKLDWSVEAAVAEFSRARPPGIYKGDYLDDLFGRYGDSEDTPAPPPLPDWC 164
BLAST of mRNA-capping enzyme vs. Ensembl Sea Lamprey
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:405803]) HSP 1 Score: 148.288 bits (373), Expect = 6.514e-41 Identity = 72/188 (38.30%), Postives = 115/188 (61.17%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFS---LTSFLASKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWA 611
P +WL CP G ++ + F+P KT L ++D ++P RF+ L+ +L S+K E +G++++LTNT ++Y+ E+ D I Y +++ GH P + F+ C + + K+IGVHCTHG+NRTGF++ ++L EK D ++ AV F++ARPPGI++ DYLD L Y D + P P+W
Sbjct: 7 PPRWLHCPRRGQLVAERFLPLKTMLSTRYDDQVPEECRFNPSMLSVYLKSRKVE-MGVLIDLTNTNRFYDKKEIEDAGITYFKLYCRGHGECPLPEHTQVFVKFCDNYIKKNPQKIIGVHCTHGFNRTGFLICAYLVEKLDWSVEAAVAEFSRARPPGIYKGDYLDDLFGRYGDSEDTPAPPPLPDWC 193
BLAST of mRNA-capping enzyme vs. Ensembl Sea Lamprey
Match: dusp11 (dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) [Source:ZFIN;Acc:ZDB-GENE-050417-226]) HSP 1 Score: 105.531 bits (262), Expect = 2.846e-26 Identity = 67/188 (35.64%), Postives = 102/188 (54.26%), Query Frame = 3
Query: 21 NKFMARGYRGNFDPPSKWLDCPNIGTIIHDM-FVPFKTPLDQKFDPEIPP-ANRFSLTSFLA--SKKSEKVGMIVNLTNTKKYYNVNEL-DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFG--DKVIGVHCTHGYNRTGFMVVSFL--CEKCDLELDVAVELFAKARPPGIFRQDYLDGLR 557
+K++ G P +W + +G + F+ FK PL Q + I + S L S+++E +G++++LTNT +YY+ EL I+Y++IF GHE P+ K + F F G DK+IGVHCTHG NRTG++V +L CEK +E D A++ F AR I R++YL L+
Sbjct: 16 SKYIPEKKGGKLQSPIRWENYVPVGKRVKGTRFISFKVPLRQNYSIRIQSEVTQMSPLGLLENISRQNESLGLVIDLTNTTRYYDPKELTKAGIKYEKIFTMGHEV-PNDKTIAHFKETIKTFIGENGENDKLIGVHCTHGVNRTGYLVCRYLIDCEK--MEPDEAIQAFNSARGHSIERENYLKDLK 200
BLAST of mRNA-capping enzyme vs. Ensembl Yeast
Match: CEG1 (Guanylyltransferase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide; human homolog RNGTT can complement yeast ceg1 null mutant [Source:SGD;Acc:S000003098]) HSP 1 Score: 95.1301 bits (235), Expect = 3.279e-21 Identity = 102/359 (28.41%), Postives = 150/359 (41.78%), Query Frame = 3
Query: 1119 FPGTQPVSMTHENV--KLLTKKKYLVSWKPDGVRYMIVVI-----GSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNL--------ILDGEMVIDI--VKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEK----RKFLPQDAKAQESFGILMK----DFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEY-------VKKDGQKPRYV--------------GNLFVNGQ----QEPF------------AKIEDISREDRE---------KYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
FPG+QPVS H +V KLL Y V K DG+R ++ ++ G +M+DR+N + V FP P + L +LDGE+VI + + R+L+FD + IN + RL + E + R P F I MK + +K + L DKL H DGLIF P K GK+ LKWKP + N++DF L + + KD + Y G VN + +PF K ++S D E N +I+EC N+ + WE++R R DK + N+ +V ++ESI + V
Sbjct: 43 FPGSQPVSFQHSDVEEKLLA-HDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYLVNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRCLTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTF-PFKISMKHMDFSYQLVKVAKSL------DKLPHLSDGLIFTPV-KAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDDRNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSDEEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSV 392
BLAST of mRNA-capping enzyme vs. Ensembl Nematostella
Match: EDO40094 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S832]) HSP 1 Score: 209.149 bits (531), Expect = 4.670e-59 Identity = 125/310 (40.32%), Postives = 177/310 (57.10%), Query Frame = 3
Query: 1068 ENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPM--SPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDA---KAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
E ++ + + K GFPG+QPVSM +N++ L +K Y VSWK DG RYM++V+G VY++DRDN VF + FP +P EH + ++DGEMV D D PR+L +DI+ F P + ++ F Q K QE F I K F+ ++ T + + + KL HE DGLIF PA ED Y+ G+ LKWKP NS+DF LK VK++G PR VG L V G PFA+I+ +++E + YNN+++EC ++ +K+W+ +R R DKS N T A + ESI NPV
Sbjct: 251 EEIQDMCQEMCKFELNGFPGSQPVSMDRQNIRFLHEKDYRVSWKADGTRYMMLVVGEGQVYLIDRDNAVFSAPQFRFPQRKNPREHIFDTLIDGEMVFDKEGDKIHPRYLAYDII--------KFQVPGK--IVPGHAPCATCFDVQQGLLNKTQEPFSIRAKQFFPLEKTAWILE-HWVPKLTHENDGLIFNPA--EDPYLPGRQASVLKWKPHTLNSVDFILKIATVKQEGCLPRSVGYLMVGGFDRPFAEIK-VTKELK-AYNNRVIECTWD--NKEWKFLRVREDKSHANAYTTAQGVCESIRNPV 543 HSP 2 Score: 168.703 bits (426), Expect = 9.265e-45 Identity = 84/193 (43.52%), Postives = 124/193 (64.25%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSL---TSFLASKKSEKVGMIVNLTNTKKYYNVNELDGI--IRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIY-DFDGKLIAPKRPEWASLEE 623
P +WL+CP +I D F FKTPL ++D +IP ANRF L S+ + K KVG+I++LTNT ++Y+ NEL+ + I++ ++ GH P Q FI IC ++D+ ++IGVHCTHG+NRTGF+++S+L EK D ++ AVE F K RPPGI++Q+YL+ L + Y D AP+ P+W +E
Sbjct: 4 PPRWLNCPRKSQVIADRFFAFKTPLSSRYDDQIPEANRFQLPMLCSYFQTLKV-KVGLIIDLTNTNRFYDKNELERLTGIKHVKLQCRGHGETPSPDQTQLFINICCRYWDKNPGELIGVHCTHGFNRTGFLIISYLVEKEDWSIEAAVECFTKCRPPGIYKQEYLNELSRRYGDSADPPSAPELPDWCYEDE 195
BLAST of mRNA-capping enzyme vs. Ensembl Nematostella
Match: EDO47609 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLI3]) HSP 1 Score: 81.2629 bits (199), Expect = 5.472e-18 Identity = 56/172 (32.56%), Postives = 91/172 (52.91%), Query Frame = 3
Query: 69 KWLDCP--NIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLA--SKKSEKVGMIVNLTNTKKYYNVNEL--DGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRK 560
+W D N I + FKTPL N F+ T + +++ K+G++++ T T +YY+ E +GII Y ++ GH P +K + +F F D+ G ++G+HCTHG NRTG+MV +L + C E + A+E F +AR + R++YL+ L+K
Sbjct: 1 RWRDYSTCNWTPIEGQRIIIFKTPLSN--------VNMFTPTDLIEQLAQREMKLGLVLDFTFTTRYYDPREFTAEGII-YKKMMCAGH-VIPKKKDIKRFEDEVKNFLENDKTGS-LVGIHCTHGVNRTGYMVCRYLIDCCGYEPEKAIEAFNQARGHPLERENYLEDLKK 161
BLAST of mRNA-capping enzyme vs. Ensembl Medaka
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:101173353]) HSP 1 Score: 239.58 bits (610), Expect = 1.579e-70 Identity = 136/329 (41.34%), Postives = 193/329 (58.66%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPM--SPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKR---KFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EG++ V+ V ++ +++ + + + +K GFPG QPVSM +N+ L++ Y VSWK DG RYM+V+ G D VYMLDRDN VF + L FP P H SN +LDGEM+ID V PR+LI+DI+ N VG +F RL I+ EII R Q K +E F + K F+DI + KL +G F ++ HE+DGLIFQP + Y +G+ LKWKPP NS+DF LK V +G + VG L+V PFAK++ + ++ ++Y+NKI+EC + + W +R+R DKS PN A + +SI PV
Sbjct: 154 FLEGITVKGVTQVTSQPKLGEIQRMCQKMAEWDKSGFPGAQPVSMDRQNLNFLSQNPYKVSWKADGTRYMMVINGKDEVYMLDRDNSVFHIANLEFPFRKDPRVHLSNTLLDGEMIIDKVNGQPVPRYLIYDIIKFNGQPVGQCDFNIRLLCIEKEIISPRMEKMKTGQIDKTKEPFSVRNKPFFDIHASRKLLEGSFTSQVSHEVDGLIFQPCGR---YKAGRCDDILKWKPPNLNSVDFRLKITKVGGEGLLTQTVGLLYVGNYDMPFAKMK--ATKELKQYDNKIIECTF--ANNTWVFMRQRVDKSFPNSYDTAMAVCKSIQEPV 475 HSP 2 Score: 85.1149 bits (209), Expect = 7.356e-17 Identity = 40/100 (40.00%), Postives = 56/100 (56.00%), Query Frame = 3
Query: 315 HETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
H P ++ F +C F + ++IGVHCTHG+NRTGF++ ++L EK D + AV F AR PGI++ DYL L R+ D AP P+W
Sbjct: 13 HGECPSKEITATFTRLCELFIQKNPSELIGVHCTHGFNRTGFLICAYLVEKMDWSTEAAVATFTLARAPGIYKGDYLKELFRRYGDEKDAPPAPALPDWC 112
BLAST of mRNA-capping enzyme vs. Ensembl Medaka
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:101173353]) HSP 1 Score: 238.81 bits (608), Expect = 1.662e-69 Identity = 136/329 (41.34%), Postives = 193/329 (58.66%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPM--SPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKR---KFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EG++ V+ V ++ +++ + + + +K GFPG QPVSM +N+ L++ Y VSWK DG RYM+V+ G D VYMLDRDN VF + L FP P H SN +LDGEM+ID V PR+LI+DI+ N VG +F RL I+ EII R Q K +E F + K F+DI + KL +G F ++ HE+DGLIFQP + Y +G+ LKWKPP NS+DF LK V +G + VG L+V PFAK++ + ++ ++Y+NKI+EC + + W +R+R DKS PN A + +SI PV
Sbjct: 235 FLEGITVKGVTQVTSQPKLGEIQRMCQKMAEWDKSGFPGAQPVSMDRQNLNFLSQNPYKVSWKADGTRYMMVINGKDEVYMLDRDNSVFHIANLEFPFRKDPRVHLSNTLLDGEMIIDKVNGQPVPRYLIYDIIKFNGQPVGQCDFNIRLLCIEKEIISPRMEKMKTGQIDKTKEPFSVRNKPFFDIHASRKLLEGSFTSQVSHEVDGLIFQPCGR---YKAGRCDDILKWKPPNLNSVDFRLKITKVGGEGLLTQTVGLLYVGNYDMPFAKMK--ATKELKQYDNKIIECTF--ANNTWVFMRQRVDKSFPNSYDTAMAVCKSIQEPV 556 HSP 2 Score: 149.058 bits (375), Expect = 1.889e-37 Identity = 75/188 (39.89%), Postives = 112/188 (59.57%), Query Frame = 3
Query: 60 PPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKV--GMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
PP +WL+CP G II D F+P KT L ++D ++ ANRF + KS KV G++V+LTNT ++Y+ N+++ I+Y ++ +GH P ++ F +C F + ++IGVHCTHG+NRTGF++ ++L EK D + AV F AR PGI++ DYL L R+ D AP P+W
Sbjct: 6 PPPRWLNCPRKGHIIADKFLPMKTMLGPRYDDQVEEANRFHPSMLFNHLKSLKVKMGLLVDLTNTNRFYDRNDIEKEGIKYVKLACKGHGECPSKEITATFTRLCELFIQKNPSELIGVHCTHGFNRTGFLICAYLVEKMDWSTEAAVATFTLARAPGIYKGDYLKELFRRYGDEKDAPPAPALPDWC 193
BLAST of mRNA-capping enzyme vs. Ensembl Medaka
Match: rngtt (RNA guanylyltransferase and 5'-phosphatase [Source:NCBI gene;Acc:101173353]) HSP 1 Score: 238.81 bits (608), Expect = 2.915e-69 Identity = 136/329 (41.34%), Postives = 193/329 (58.66%), Query Frame = 3
Query: 1017 FMEGVS--KVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPM--SPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKR---KFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
F+EG++ V+ V ++ +++ + + + +K GFPG QPVSM +N+ L++ Y VSWK DG RYM+V+ G D VYMLDRDN VF + L FP P H SN +LDGEM+ID V PR+LI+DI+ N VG +F RL I+ EII R Q K +E F + K F+DI + KL +G F ++ HE+DGLIFQP + Y +G+ LKWKPP NS+DF LK V +G + VG L+V PFAK++ + ++ ++Y+NKI+EC + + W +R+R DKS PN A + +SI PV
Sbjct: 261 FLEGITVKGVTQVTSQPKLGEIQRMCQKMAEWDKSGFPGAQPVSMDRQNLNFLSQNPYKVSWKADGTRYMMVINGKDEVYMLDRDNSVFHIANLEFPFRKDPRVHLSNTLLDGEMIIDKVNGQPVPRYLIYDIIKFNGQPVGQCDFNIRLLCIEKEIISPRMEKMKTGQIDKTKEPFSVRNKPFFDIHASRKLLEGSFTSQVSHEVDGLIFQPCGR---YKAGRCDDILKWKPPNLNSVDFRLKITKVGGEGLLTQTVGLLYVGNYDMPFAKMK--ATKELKQYDNKIIECTF--ANNTWVFMRQRVDKSFPNSYDTAMAVCKSIQEPV 582 HSP 2 Score: 139.043 bits (349), Expect = 4.661e-34 Identity = 75/214 (35.05%), Postives = 112/214 (52.34%), Query Frame = 3
Query: 60 PPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKV----------------------------GMIVNLTNTKKYYNVNELDG-IIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGL-RKIYDFDGKLIAPKRPEWA 611
PP +WL+CP G II D F+P KT L ++D ++ ANRF + KS KV G++V+LTNT ++Y+ N+++ I+Y ++ +GH P ++ F +C F + ++IGVHCTHG+NRTGF++ ++L EK D + AV F AR PGI++ DYL L R+ D AP P+W
Sbjct: 6 PPPRWLNCPRKGHIIADKFLPMKTMLGPRYDDQVEEANRFHPSMLFNHLKSLKVGHSAPLAVELLNLLKAPFIVLFIHQVKMGLLVDLTNTNRFYDRNDIEKEGIKYVKLACKGHGECPSKEITATFTRLCELFIQKNPSELIGVHCTHGFNRTGFLICAYLVEKMDWSTEAAVATFTLARAPGIYKGDYLKELFRRYGDEKDAPPAPALPDWC 219
BLAST of mRNA-capping enzyme vs. Ensembl Medaka
Match: dusp11 (dual specificity phosphatase 11 [Source:NCBI gene;Acc:101156622]) HSP 1 Score: 100.138 bits (248), Expect = 1.605e-22 Identity = 57/173 (32.95%), Postives = 95/173 (54.91%), Query Frame = 3
Query: 57 DPPSKWLDCPNIGTIIHDM-FVPFKTPLDQKFDPEIPPANRFSLTSFL--ASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRK 560
D P +WLD +G + F+ FK PL Q +P ++ F L S+ +++G+I++LT T +YY++ +L + + +IF GH+ P + F + F ++ DK+IGVHCTHG NRTG+++ +L + ++ AV+LF +R I RQ+YL L +
Sbjct: 11 DIPDRWLDYTAVGRRLQGTRFIAFKVPLKQALTRMLPRSSVFGPWELLDAVSRDRQELGLIIDLTFTSRYYDLQDLPASLMFVKIFTAGHQV-PSDGTILSFKRAVNNFLRDNQDNDKLIGVHCTHGLNRTGYLICRYLIDVDGMDPAEAVKLFNSSRGHAIERQNYLKDLHR 182
BLAST of mRNA-capping enzyme vs. Ensembl Medaka
Match: dusp11 (dual specificity phosphatase 11 [Source:NCBI gene;Acc:101156622]) HSP 1 Score: 97.8265 bits (242), Expect = 1.672e-21 Identity = 55/169 (32.54%), Postives = 93/169 (55.03%), Query Frame = 3
Query: 69 KWLDCPNIGTIIHDM-FVPFKTPLDQKFDPEIPPANRFSLTSFL--ASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFF--DRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRK 560
+WLD +G + F+ FK PL Q +P ++ F L S+ +++G+I++LT T +YY++ +L + + +IF GH+ P + F + F ++ DK+IGVHCTHG NRTG+++ +L + ++ AV+LF +R I RQ+YL L +
Sbjct: 56 RWLDYTAVGRRLQGTRFIAFKVPLKQALTRMLPRSSVFGPWELLDAVSRDRQELGLIIDLTFTSRYYDLQDLPASLMFVKIFTAGHQV-PSDGTILSFKRAVNNFLRDNQDNDKLIGVHCTHGLNRTGYLICRYLIDVDGMDPAEAVKLFNSSRGHAIERQNYLKDLHR 223
BLAST of mRNA-capping enzyme vs. Planmine SMEST
Match: SMESG000080877.1 (SMESG000080877.1) HSP 1 Score: 798.119 bits (2060), Expect = 0.000e+0 Identity = 398/462 (86.15%), Postives = 402/462 (87.01%), Query Frame = 3
Query: 30 MARGYRGNFDPPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYDFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEACEVERMETEVIDSASNLTEFKITSLPEKQIEIPQSCIIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCIN 1415
MARGYRGNFDPPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYDFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEA R +SC + V + ALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDI+ N
Sbjct: 1 MARGYRGNFDPPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYDFDGKLIAPKRPEWASLEEKKELCYVQHSLNVDKEDTKQLSQPKETSRSPKSHEIQDMEAFMYYR----------------------------KSCSCQANVTS---------------------ALSEQSVKIVSIPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIIISN 413
BLAST of mRNA-capping enzyme vs. Planmine SMEST
Match: SMESG000024208.1 (SMESG000024208.1) HSP 1 Score: 322.783 bits (826), Expect = 6.347e-104 Identity = 161/337 (47.77%), Postives = 232/337 (68.84%), Query Frame = 3
Query: 975 IPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKR-KFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
+P + +P FMEGVS VS V + + +++ VR+LM KFGF G QPVSM EN++ L +KK++VSWKPDG RY+++++ VYM DR+N V++V+ + FP S + H + ++DGEMVIDI + FPR+LI+D++ ++ VG F RL +IK+ II+ R KF+ D K++ESFG+ +KDFW I + LW+G F+ KL H+MDGLIFQ A + D Y G+ + LKWKP E NS+DF LKCE+ + + ++ G L+V ++E FAKI++ISRE REKYNNKI+EC Y+ K W +IRER+DKS PN+++ A ++M+SIL PV
Sbjct: 48 VPKTKPVKAATIPSFMEGVSGVSPVTEEDVIGELQKKVRDLMNSKKFGFVGCQPVSMMRENLESLKEKKFMVSWKPDGTRYIMLILSDSKVYMFDRNNCVYKVDNINFPNSKNKGHFDTLVDGEMVIDIYNEKRFPRYLIYDVMSLDGKYVGNKIFVERLKIIKNFIIDCRNKFVRTDKKSKESFGVRIKDFWPINRAKDLWEGQFRTKLSHKMDGLIFQQAGENDFYEQGRCYSILKWKPAELNSVDFHLKCEHQRGENKESTNTGYLYV-SEREHFAKIDNISRELREKYNNKIVECTYDVEKKIWTIIRERSDKSFPNHISTAKSVMKSILEPV 383
BLAST of mRNA-capping enzyme vs. Planmine SMEST
Match: SMESG000024237.1 (SMESG000024237.1) HSP 1 Score: 287.345 bits (734), Expect = 1.771e-89 Identity = 146/336 (43.45%), Postives = 209/336 (62.20%), Query Frame = 3
Query: 975 IPPSATFCPPKVPKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHHSNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
+P + +P FMEGVS VS V + +E +++ VR+LM KFGF G QPVSM EN++ L +KK++VSWKPDG RY+++++ VYM DR+N V++V+ + FP S + H + ++DGEMVIDI + ++ KF+ D K+ ESFG+ +KDFW I + LW+G F+ KL H+MDGLIFQ A + D Y G+ + LKWKP E NS+DF LKCE+ + + ++ G L+V ++E FAKI++ISRE REKYNNKI+EC Y+ K W +IRER+DKS PN+++ A ++M+SIL PV
Sbjct: 48 VPKTKPVKAATIPSFMEGVSGVSPVTEEDVIEELQKKVRDLMNSKKFGFVGCQPVSMMRENLESLKEKKFMVSWKPDGTRYIMLILSDSKVYMFDRNNCVYKVDNINFPNSKNKGHFDTLVDGEMVIDIYNE------------------------------------KRNKFVRTDKKSNESFGVRIKDFWPINRAKDLWEGQFRTKLSHKMDGLIFQQAGENDFYEQGRCYSILKWKPAELNSVDFHLKCEHQRGENKESTNTGYLYV-SEREHFAKIDNISRELREKYNNKIVECTYDVEKKIWTIIRERSDKSFPNHISTAKSVMKSILEPV 346
BLAST of mRNA-capping enzyme vs. Planmine SMEST
Match: SMESG000080735.1 (SMESG000080735.1) HSP 1 Score: 140.198 bits (352), Expect = 1.145e-38 Identity = 66/124 (53.23%), Postives = 88/124 (70.97%), Query Frame = 3
Query: 1611 MDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSPNYVTVANNIMESILNPV 1982
MDGLIFQP +D Y G LKWKPPE NSIDF L+CEY P +VG L+V+G++ F KI+ I ++ REKYNNKI+EC+ N+ + +W +IRERTDKS PN+++ A +++ESI NPV
Sbjct: 1 MDGLIFQPCGPDDFYEPGTCPFILKWKPPELNSIDFLLRCEYETGVDIVPNFVGKLYVSGEKMFFGKIDHIKKDLREKYNNKIVECVLNRTTLQWVIIRERTDKSKPNHISTAKSVLESIGNPV 124
BLAST of mRNA-capping enzyme vs. Planmine SMEST
Match: SMESG000080768.1 (SMESG000080768.1) HSP 1 Score: 119.398 bits (298), Expect = 1.677e-27 Identity = 57/182 (31.32%), Postives = 105/182 (57.69%), Query Frame = 3
Query: 63 PSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFSLTSFLASKKSEKVGMIVNLTNTKKYYNVNELDGIIRYDQIFLEGHETYPDQKQVDKFIVICSYFFDRFGDKVIGVHCTHGYNRTGFMVVSFLCEKCDLELDVAVELFAKARPPGIFRQDYLDGLRKIYDFDGKLIAPKRPEW 608
P W+ P G +I D+F P KT LD+++ + +N F++ F + ++ ++++L +++KYY ++ +++ +I + G E P K + FI I + + + I VHCT GYN TGFM+V++L + ++L+ A+E+FAK+RPPGI+ +L L ++ + + P P+W
Sbjct: 7 PRGWIHSPRRGYMIADLFYPMKTVLDERYKDLVTESNSFNVGYFTEQAEMYQIVLLIDLIDSEKYYKSKDMKDVLQI-KITVPGLEALPTSKHITAFIKIVNKNIELDKEGKIAVHCTQGYNLTGFMIVNYLVTQRGMQLNEALEIFAKSRPPGIYNPAFLKHLLNLHQPNLPIHYPTPPKW 187 HSP 2 Score: 116.316 bits (290), Expect = 1.350e-26 Identity = 102/381 (26.77%), Postives = 174/381 (45.67%), Query Frame = 3
Query: 819 LPEKQIEIPQSC----IIEKVVAAKPSIIEKIEDIGKNIISSVTSALSEQSVKIVSIPPSATF--CPPKV--PKFMEGVSKVSAVLDVTEMENVRQHVRNLMKVNKFGFPGTQPVSMTHENVKLLTKKKYLVSWKPDGVRYMIVVIGSDIVYMLDRDNFVFRVERLYFPMSPTEHH--SNLILDGEMVIDIVKDIGFPRFLIFDIVCINSVNVGIFNFPHRLFLIKSEIIEKRKFLPQDAKAQESFGILMKDFWDIKTTEKLWDGDFQDKLLHEMDGLIFQPAEKEDVYISGKNHHSLKWKPPEYNSIDFFLKCEYVKKDGQKPRYVGNLFVNGQQEPFAKIEDISREDREKYNNKIMECIYNKISKKWELIRERTDKSSP 1931
LPE + + +SC +I V K +ED S T +L ++ +SI P P K+ P+F+ ++ + D + +R+ ++ L+K +GF G + +T + K++ +W+ DG RY++ +I +YM+D N F++E L FP + N ++DGE+V + + + FLI DI + + + ++ RL I+ I + RK E F I++K + + W + + HEM GLIF P G+ + W P I F LK + K QK ++G LF G EPF KI ++ +++E Y+ KI+ C NK +W + + D + P
Sbjct: 467 LPELEDKKEESCSKAELIHSPVIGKKRSSALLED------SFKTGSLKKRRTDDISIEPECKLEIKPKKIERPEFLVYMN----LKDEKTVFRLREELQYLLKTTMYGFVGHKYKLLTGKYYNHFMSKEFYHTWRTDGKRYLMFIINESHIYMIDEQNRYFKIENLKFPHPHRKGKFLINTVIDGELVAETISSKTYYLFLISDIYALGGIRLYHESYSKRLDKIQEFITDVRKERIDLKSVVEPFDIMIKTISESRELVNTW-VRLRKTIPHEMTGLIFYPYNDISYTFLGRATIYI-WIPTFSRYIKFKLKA-VIDKHNQK--WIGLLFAYGTLEPFTKIRYLTTKEKETYDGKIVRCYLNK--GRWNISKHNPDDTIP 830 The following BLAST results are available for this feature:
BLAST of mRNA-capping enzyme vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of mRNA-capping enzyme vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of mRNA-capping enzyme vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 3
BLAST of mRNA-capping enzyme vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of mRNA-capping enzyme vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of mRNA-capping enzyme vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of mRNA-capping enzyme vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of mRNA-capping enzyme vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of mRNA-capping enzyme vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of mRNA-capping enzyme vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of mRNA-capping enzyme vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of mRNA-capping enzyme vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of mRNA-capping enzyme vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of mRNA-capping enzyme vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30014011 ID=SMED30014011|Name=mRNA-capping enzyme|organism=Schmidtea mediterranea sexual|type=transcript|length=2087bpback to top protein sequence of SMED30014011-orf-1 >SMED30014011-orf-1 ID=SMED30014011-orf-1|Name=SMED30014011-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=666bp MARGYRGNFDPPSKWLDCPNIGTIIHDMFVPFKTPLDQKFDPEIPPANRFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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